AU2001292056A1 - Air samplers - Google Patents
Air samplersInfo
- Publication number
- AU2001292056A1 AU2001292056A1 AU2001292056A AU2001292056A AU2001292056A1 AU 2001292056 A1 AU2001292056 A1 AU 2001292056A1 AU 2001292056 A AU2001292056 A AU 2001292056A AU 2001292056 A AU2001292056 A AU 2001292056A AU 2001292056 A1 AU2001292056 A1 AU 2001292056A1
- Authority
- AU
- Australia
- Prior art keywords
- air
- liquid
- sampler
- entraining
- airflow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Description
AIR SAMPLERS
This invention relates to air samplers and particularly, but not exclusively, relates to air samplers for sampling the ambient atmosphere, in order to detect and measure contamination that may be present.
The present invention provides an air sampler for collecting cumulative samples from large volumes of air in small volumes of liquid, (e.g. particles from 100 cubic metres of air in about lOmls of liquid).
In addition sequential samples may be taken automatically. These samples may be kept cool to preserve them for later analysis.
According to the present invention, an air sampler comprises an air flow chamber having an air inlet and an air outlet, means for introducing liquid into air flowing through the chamber so as to entrain any particles present in the airflow, means for collecting the entraining liquid and any entrained particles therein, and for depositing the same in a collection vessel, means for returning collected entraining liquid to the airflow chamber, means for detecting the presence of bubbles in the returning liquid, and operable to introduce fresh entraining liquid into air flowing through the airflow chamber, and means for collecting a sample of the entraining liquid and any particles therein, which may be present in said collection vessel.
An embodiment of the invention will now be described by way of example only, with reference to the accompanying drawings wherein: -
Figure 1 is a diagrammatic illustration of the air sampler, and
Figure 2 is an enlarged detail of part of Figure 1.
With reference to Figure 1, an air sampler 1 for use in analysing biological analytes, comprises an airflow chamber 2 having an air inlet 3 and an air outlet 4.
An air mover 5, conveniently an extraction fan, draws ambient air at 500 to 1000 litres per minute in through the inlet 3, and then through the chamber 2, to discharge it back t to atmosphere by way of an outlet 6.
The air flow chamber 2 houses a cyclone air/liquid separator 7 through which the inflowing air is caused to pass.
Means comprising an injector 8 introduces liquid into air flowing through the air inlet 3. The cyclone separator 7 extracts this liquid and any entrained particles therein and discharges it from the airflow chamber 2, initially by way of a duct 9, where it is then conveyed to a collection vessel 12, by way of one channel of a single three channel peristaltic pump 13 and a duct 14. The collection vessel 12 is housed within a Peltier- cooled holder 15 to maintain the contents of the vessel 12 within the 2°C - 8°C range which is desirable for mamtaining the integrity of biological analytes such as bacteria or proteins. Analyses such as PCR, (Polymerase Chain Reaction), culture or agar plates or immunoassays may be performed on the collected samples.
The holder 15 also houses bottles 16,17,18 and 19.
A large pore (e.g. 25 micron) filter unit 25 is fitted in the duct 9 to remove large particulates present in the collected air which are unsuitable for a particular assay. Diesel soot, for example. The filter unit 25 will, however, allow soluble enalytes or small particulate analytes, such as bacteria and viruses, to pass through.
The sampler 1 further comprises recirculation means for returning collected entraining liquid from the vessel 12 to the airflow chamber 2 where it is discharged to air flowing into the cyclone 7. These means comprise an extraction line 30, a bubble detector 31, a pinch valve 32, and a discharge line 33 which passes through another of the three channels of pump 13. The bubble detector 31 is connected to electronic circuitry, which includes, a programmable timer 34, by way of an electronic signal line 35. The circuitry incorporates a controlling microprocessor.
A reservoir 36 houses entrainment liquid 37, comprising de-ionised water containing 0.01% of a non-ionic detergent such as Tween-20 and lOmM HEPES N-2-hydroxyethyl piperazine - N'-2 ethane sulphonic acid buffer; pH 7.5. Line 33 draws this liquid frδm the reservoir 36 by way of the pinch valve 32, and passes it, via a needle-gauge stainless tube, which forms the injector 8, into the air flow inlet 3, at about 2.0millilitres per minute.
Aerosol particles entrained into the liquid stream exit from the air flow chamber 2, (via a standard Luer-fitting port), and through pump 13, to be deposited in the collection vessel 12.
The collection fluid is re-circulated from the vessel 12 back into the airflow chamber 2 by way of line 30, bubble detector 31, pinch valve 32, pump 13 and line 33. This continues until the level of liquid within the vessel 12 falls to a point whereby air rather than liquid is drawn into line 30. When the entrained air reached the bubble detector 31 , an electronic signal is sent via line 35 to the microprocessor in the electronic circuitry which includes programmable timer 34. The microprocessor then operates the pinch valve 32, so that, for a pre-set period of time, fresh collection fluid is drawn from the reservoir 36 and discharged into the airflow chamber 2, by way of line 33, pinch valve 32, pump 13, line 33 and injector 8.
After the set period the pinch valve 32 is operated so that the system is restored to the re-circulation mode, which continues until the liquid level falls again in vessel 12, and air is again entrained.
A time delay is built into the system so as to allow fluid from the re-charged vessel 12 to reach the bubble detector 31.
In addition, a start up routine is programmed into timer 34 to allow initial charging of the system.
As mentioned above, pump 13 is a single three channel pump. Two of the channels of the pump are used for pumping liquid into and out of the airflow chamber 2. The remaining channel is used to draw off small volumes of liquid for discharge to a selected one or more of the sample bottles 16, 17, 18 and 19, via line 39 and three-way valves 40, 41 and 42. (See Figure 2).
In this example, the flow rate of entraining liquid into the airflow chamber 2 and cyclone 7 is ca.2 millilitres per minute, the sample for analysis may be drawn off at a much lower rate of ca.50 microlitres per minute, using smaller bore tubing. This allows an integrated sample of aerosol particles to be collected for up to 8 hours into a standard
25ml "universal" bottle. In the present embodiment there are four sample bottles, (16- 19) so monitoring the air for an 8-hour working shift, or a full 24 hour day, is possible without the need for an operator to be present. In addition, the programmable timer 34 allows the equipment to be positioned and then to be activated automatically at set times up to one week in advance. For 4 samples, the 3-way valves (40-42) are needed to direct the sample flow.
The collected samples are subsequently analysed in the conventional manner.
Claims (8)
1. An air sampler comprising an airflow chamber having an air inlet and an air outlet, means for introducing liquid into air flowing through the chamber so as'to entrain any particles present in the airflow, means for collecting the entraining
5 liquid and any entrained particles therein, and for depositing the same in a collection vessel, means for returning collected entraining liquid to the airflow chamber, means for detecting the presence of bubbles in the returning liquid, and operable to introduce fresh entraining liquid into air flowing through the airflow chamber, and means for collecting a sample of the entraining liquid and any
LO particles therein, which may be present in said collection vessel.
2. An air sampler as claimed in Claim 1, provided with programmable timer means operable to obtain air samples at pre-determined times.
L5 3. An air sampler as claimed in Claim 2, incorporating a microprocessor for controlling the sampler.
4. An air sampler as claimed in Claim 1, 2 or 3, wherein said fresh entraining liquid is introduced when the level in the collection vessel falls to a level whereby air
10 is entrained in the returned collected liquid, and is detected by the said means for detecting bubbles.
5. An air sampler as claimed is any one of Claims 1 to 4, provided with a plurality of sampler bottles and means for drawing-off liquid so as to divert it to the
15 bottles.
6. An air sampler as claimed in any one of Claims 1 to 5, provided with a three channel pump and a plurality of sample bottles, wherein two of the channels of the pump are used for pumping liquid into and out of the airflow chambers and 0 the third channel is used to draw-off liquid and divert it to a selected sample bottle.
7. An air sampler as claimed in Claim 6, wherein the pumps is a single, three channel peristaltic pump. c
8. An air sampler, substantially as herein before described, with reference to the accompanying drawings.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0024227.1A GB0024227D0 (en) | 2000-10-04 | 2000-10-04 | Air samplers |
GB0024227.1 | 2000-10-04 | ||
PCT/GB2001/004387 WO2002029380A2 (en) | 2000-10-04 | 2001-10-01 | Air samplers |
Publications (2)
Publication Number | Publication Date |
---|---|
AU2001292056A1 true AU2001292056A1 (en) | 2002-06-27 |
AU2001292056B2 AU2001292056B2 (en) | 2005-04-14 |
Family
ID=9900611
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001292056A Ceased AU2001292056B2 (en) | 2000-10-04 | 2001-10-01 | Air samplers |
AU9205601A Pending AU9205601A (en) | 2000-10-04 | 2001-10-01 | Air samplers |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU9205601A Pending AU9205601A (en) | 2000-10-04 | 2001-10-01 | Air samplers |
Country Status (14)
Country | Link |
---|---|
US (1) | US6851459B2 (en) |
EP (1) | EP1322934B1 (en) |
JP (1) | JP4018980B2 (en) |
CN (1) | CN100335882C (en) |
AT (1) | ATE421683T1 (en) |
AU (2) | AU2001292056B2 (en) |
CA (1) | CA2424631C (en) |
DE (1) | DE60137517D1 (en) |
GB (1) | GB0024227D0 (en) |
HK (1) | HK1062046A1 (en) |
IL (2) | IL155147A0 (en) |
NZ (1) | NZ524960A (en) |
RU (1) | RU2242738C1 (en) |
WO (1) | WO2002029380A2 (en) |
Families Citing this family (25)
Publication number | Priority date | Publication date | Assignee | Title |
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US6765490B2 (en) | 2001-10-23 | 2004-07-20 | Steven W. Lopez | System and methods for detecting harmful agents within contents of mail |
WO2003095983A1 (en) * | 2002-05-10 | 2003-11-20 | Abb Patent Gmbh | Method and device for taking indoor air samples |
JP2005526522A (en) * | 2002-05-20 | 2005-09-08 | ノースロップ グラマン コーポレーション | Point source biological material detection system |
GB0228862D0 (en) * | 2002-12-11 | 2003-01-15 | Smiths Group Plc | Particle collection apparatus and methods |
CN1208113C (en) * | 2003-05-01 | 2005-06-29 | 东南大学 | Device for collecting micro-substance in air and collection method thereof |
GB0319671D0 (en) | 2003-08-21 | 2003-09-24 | Secr Defence | Apparatus for processing a fluid sample |
US7370543B2 (en) | 2003-10-17 | 2008-05-13 | The United States Of America As Represented By The Department Of Health And Human Services | Air-sampling device and method of use |
US7837937B2 (en) | 2004-07-09 | 2010-11-23 | Hamilton Sundstrand Corporation | Biological agent detector |
US7703671B2 (en) * | 2005-01-28 | 2010-04-27 | Arrowhead Center, Inc. | Monitoring device and security system |
FR2905379B1 (en) * | 2006-09-04 | 2008-11-07 | Bertin Technologies Soc Par Ac | DEVICE FOR COLLECTING AND SEPARATING PARTICLES AND MICROORGANISMS IN AMBIENT AIR |
US7964018B2 (en) * | 2007-12-03 | 2011-06-21 | Samsung Electronics Co., Ltd. | Apparatus and method for collecting and detecting airborne particles |
KR101318930B1 (en) * | 2008-07-16 | 2013-10-17 | (주)바이오니아 | Collecting Apparatus of Biological Material for Mounting on Remotely Piloted Vehicle |
US8209066B2 (en) * | 2010-02-11 | 2012-06-26 | Bae Systems Information And Electronic Systems Integration Inc. | Environmental control system for precision airborne payloads |
DE102010037425B4 (en) * | 2010-09-09 | 2012-06-06 | Eurofins Wej Contaminants Gmbh | Method for taking a representative sample of bulk particles to determine mycotoxin contamination of the bulk material |
WO2012112040A1 (en) * | 2011-02-15 | 2012-08-23 | Uc Technologies | Detection apparatus |
RU2537174C1 (en) * | 2013-07-23 | 2014-12-27 | Федеральное государственное бюджетное учреждение науки Государственный научный центр Российской Федерации-Институт медико-биологических проблем Российской академии наук (ГНЦ РФ-ИМБП РАН) | Device and method for selection and preservation of biosamples in extreme living conditions |
EP3368227B1 (en) * | 2015-10-30 | 2021-12-08 | Seurat Technologies, Inc. | Additive manufacturing system and method |
CN107367766B (en) * | 2016-05-13 | 2020-12-15 | 深圳迈瑞生物医疗电子股份有限公司 | Liquid amount monitoring device and method for sample analyzer |
CN106085830B (en) * | 2016-08-30 | 2019-03-19 | 浙江省疾病预防控制中心 | Pathogenic microorganism collection device in air |
WO2018102116A1 (en) * | 2016-12-02 | 2018-06-07 | David brian j | Automatic air-sampling and pneumatic transport system with ball-filter hopper and integrated sampling manifold |
CN106769270B (en) * | 2017-02-16 | 2024-03-29 | 北京城市排水集团有限责任公司 | Static water surface atmosphere polluted gas sampling device and sampling method thereof |
RU2723026C1 (en) * | 2019-04-10 | 2020-06-08 | Федеральное автономное учреждение "25 Государственный научно-исследовательский институт химмотологии Министерства обороны Российской Федерации" | Miniaspirator for determination of different analytes in air |
CN111122741A (en) * | 2019-12-31 | 2020-05-08 | 安徽皖仪科技股份有限公司 | Multi-liquid-phase chromatography mobile phase switching system and switching method |
RU2750605C1 (en) * | 2020-11-11 | 2021-06-29 | ФЕДЕРАЛЬНОЕ ГОСУДАРСТВЕННОЕ БЮДЖЕТНОЕ УЧРЕЖДЕНИЕ "33 ЦЕНТРАЛЬНЫЙ НАУЧНО-ИССЛЕДОВАТЕЛЬСКИЙ ИСПЫТАТЕЛЬНЫЙ ИНСТИТУТ" Минобороны России | Device for isolating contaminated air in static inhalation chamber for sampling |
CN115491298A (en) * | 2022-10-24 | 2022-12-20 | 华北电力大学(保定) | Online monitoring device for biological aerosol |
Family Cites Families (7)
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FR2586108B1 (en) | 1985-08-07 | 1987-10-23 | Commissariat Energie Atomique | METHOD AND DEVICE FOR THE CONTINUOUS MEASUREMENT ACCORDING TO TIME OF THE CONCENTRATION OF AEROSOLS IN A GAS |
DE3937134A1 (en) * | 1989-11-08 | 1991-05-16 | Basf Ag | LABORATORY DEVICE FOR SEPARATING SUBSTANCES FROM GAS SAMPLES |
EP0668095A1 (en) | 1994-01-28 | 1995-08-23 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern | Air/liquid contacting apparatus |
GB9509577D0 (en) * | 1995-05-11 | 1995-07-05 | Martinex R & D Inc | Capillary sampling flow controller |
FR2773217B1 (en) | 1997-12-30 | 2001-06-08 | Controles Lab Et | USE OF AN INTERACTION BETWEEN A GAS AND A LIQUID FOR THE SAMPLING OF GAS AND PARTICULAR ELEMENTS FROM A GASEOUS MEDIUM FOR THEIR ANALYSIS |
GB9913443D0 (en) * | 1999-06-09 | 1999-08-11 | Air Dispersions Ltd | Sampling devices |
US6550347B2 (en) * | 2000-11-30 | 2003-04-22 | Bruce J. Bradley | Vacuum air component sampler |
-
2000
- 2000-10-04 GB GBGB0024227.1A patent/GB0024227D0/en not_active Ceased
-
2001
- 2001-10-01 CN CNB018168442A patent/CN100335882C/en not_active Expired - Fee Related
- 2001-10-01 EP EP01972278A patent/EP1322934B1/en not_active Expired - Lifetime
- 2001-10-01 IL IL15514701A patent/IL155147A0/en unknown
- 2001-10-01 US US10/381,947 patent/US6851459B2/en not_active Expired - Lifetime
- 2001-10-01 WO PCT/GB2001/004387 patent/WO2002029380A2/en active IP Right Grant
- 2001-10-01 CA CA2424631A patent/CA2424631C/en not_active Expired - Fee Related
- 2001-10-01 AU AU2001292056A patent/AU2001292056B2/en not_active Ceased
- 2001-10-01 RU RU2003112683/12A patent/RU2242738C1/en not_active IP Right Cessation
- 2001-10-01 AT AT01972278T patent/ATE421683T1/en not_active IP Right Cessation
- 2001-10-01 NZ NZ524960A patent/NZ524960A/en not_active IP Right Cessation
- 2001-10-01 DE DE60137517T patent/DE60137517D1/en not_active Expired - Lifetime
- 2001-10-01 JP JP2002532904A patent/JP4018980B2/en not_active Expired - Fee Related
- 2001-10-01 AU AU9205601A patent/AU9205601A/en active Pending
-
2003
- 2003-03-30 IL IL155147A patent/IL155147A/en not_active IP Right Cessation
-
2004
- 2004-07-13 HK HK04105107A patent/HK1062046A1/en not_active IP Right Cessation
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