WO2003090533A1

WO2003090533A1 - Plant growth regulating composition for stress- resistance and promoting growth

Info

Publication number: WO2003090533A1
Application number: PCT/CN2002/000278
Authority: WO
Inventors: Hong Tan; Xianguo Fan; Bingyao Sun; Daxiang Li
Original assignee: Sichuan Lomon Bio Technology Co., Ltd.; Chengdu Institute Of Biology Chinese Academy Of Sciences
Priority date: 2002-04-23
Filing date: 2002-04-23
Publication date: 2003-11-06
Also published as: CN1377578A; CN1157117C

Abstract

The present invention relates to a plant growth regulating composition for stress-resistance and promoting growth, comprising as active components A) a natural type abscisic acid, B) 4-chloro-indole-3-acetic acid or 4-indol-3-yl-butyric acid and optional C)1-naphthyl acetic acid, if necessary 8-hydroxyquinoline, in addition to general inert auxiliaries.

Description

Technical field of plant growth regulating composition with anti-stress and promoting effect

The present invention relates to a plant growth regulating composition containing natural abscisic acid with anti-stress and promoting effect, and its international classification number belongs to A01N37 / 06.

Background technique

For a long time, forest and grass vegetation have been severely damaged in some areas in China, especially in the western region, causing China to pay a huge price in terms of ecological environment: the area of soil erosion has reached 1.79 million square kilometers; the area of desertified land has reached 1.68 million square kilometers, and It is also expanding at a speed of 2460 square kilometers; the frequency and intensity of sandstorms are increasing; frequent floods and floods, and the contradictory benefits of water shortage are prominent. It is imperative to attach importance to afforestation and greening and improve the ecological environment. However, in artificial afforestation, it is necessary to breed high-quality seedlings and ensure a high survival rate after transplantation. Therefore, it is necessary to find a good forest tree growth and transplantation promoter.

Natural abscisic acid, S-Absci sic acid (S-ABA), common name of pesticide: S-actin. Molecular formula: C ₁₅ . 0 ₄ , the chemical name is: 5-(1 '-hydroxy-2', 6 ', 6'-trimethyl-4'-oxo-2'-cyclohexene- -yl) -3-methyl- 2-cis- 4 -trans-pentadienoic acid, the chemical structural formula is as follows

Chemical structure of S-ΛΒΑ

Abscisic acid was first extracted from cotton young fruit by Acldicott et al. In 1963. For more than three decades, scientists from various countries have conducted extensive and in-depth studies on the effects of plant physiological activities. Studies have shown that ABA is a plant's "stress-resistance-inducing factor" and "balanced growth factor", and it can initiate plant stress-resistance gene expression. Activating the plant's endogenous immune system, inducing plants to resist biotic and abiotic stresses (drought, low temperature, saline-alkali, pests and diseases), improving the plant's ability to resist drought, cold, disease and saline-alkali Agriculture, forestry, animal husbandry, landscaping, ecological vegetation restoration, etc. Although the use of ABA (1.0-500. 0 ppm) in J ^ can promote the growth and growth of young tree seedlings, and improve their stress resistance; the application in the transplanting period can improve the survival rate of seedlings. However, the duration of the ABA apple agent is relatively short, which has certain limitations on the growth promotion effect of seedlings.

Indole acetic acid, indole butyric acid are auxin plant growth regulators, they can be used in woody and herbaceous plants Cuttings of plants to accelerate root formation and increase rooting rate of plants, for example, Ye Maofu, etc. in Botany Bulletin

In 1998, S1 described the use of plant growth regulators such as NAA, IBA, and IAA to promote the rooting of Chinese rose, boxwood, Metasequoia, Metasequoia glyptostroboides, Chinese fir, and Hainan Shizi. Yan root soil like "Chinese cotton" 199 421 (5): ^12--13 page well Ji effects of different hormone combination of Keyu cotton 312 embryoid induction, which comprises using 10mg / L IAA (IBA) +0 The effect of lmg / L BAP and other treatments on seedling growth rate and fresh root weight; Lou Chengfu et al. published in "Sericulture Science" 1996 22 (4): 241-244 to promote the rooting of cuttings. The effects of the type, concentration, and treatment time of additional plant hormones in the culture medium on the somatic embryos of the spruce tree species were disclosed in Botany Bulletin 1999, 16 (1): 59-66. It was mentioned that differentiation medium containing ABA, IBA or 6-BA can increase the frequency of embryogenesis and promote cotyledon development.

Summary of the Invention

The object of the present invention is to provide a plant growth regulating composition containing natural abscisic acid, characterized in that the composition includes A) natural abscisic acid and B) 4-chloroindoleacetic acid (4-C1-IAA) or indole Butyric acid (IBA).

The plant 'growth-regulating composition of the present invention may further contain component C) naphthaleneacetic acid (NAA), and 8-hydroxyquinoline may be added if necessary.

The present inventors found that although ABA (1.0-500. 0 ppm) alone can promote the development and growth of young tree seedlings, and improve their stress resistance; the application in the transplanting period can improve the survival rate of seedlings. However, the single-agent ABA has a short holding period, which has certain limitations on the growth promotion effect of seedlings. The results of research and experiments have found that the combination of S-ABA with 4-chloroindoleacetic acid (4-CI-IAA) or indolebutyric acid (IBA) can improve the resistance of seedlings to stress and show a wider range than single use Resistant to cold, drought, salt and alkali. It also promotes the saplings and forest roots to be developed and grow robustly. Application during transplanting can improve the survival rate of saplings and promote the rapid growth of saplings and forests; it can increase the success rate of cuttings and rooting. The holding period is longer than that of ABA alone, and the effect is more significant.

And it is surprising that the composition of S-ABA, 4-chloroindoleacetic acid (4-C1-IAA) or indolebutyric acid (IBA) according to the present invention, and other plant growth regulating substances are added on this basis The growth-regulating activity of crops exceeds the expected additive effect, mainly on the one hand, increasing the active range of each ingredient; on the other hand, reducing the application concentration of natural abscisic acid and other plant growth regulators, and the combination of plant growth regulators also Good levels of plant growth regulation are obtained with single compounds below effective concentrations.

-Generally speaking, the concentration of S-ABA in the composition of the present invention is 1.0-500. 0 ppm, preferably 1. 0-100. 0 ppm, and most preferably 1. 0-50. 0 ppm; 4-chloro Use of indole acetic acid (4-C IAA) or indole butyric acid (IBA) 1-300. 0 ppm is preferably 0.1-100. 0 ppm, most preferably 1.0-40. 0 ppm; NAA concentration of 0.01-300. 0 ppm is preferably 0.1-100 0 ppm, most preferably 1.0-50. 0 ppm; 8-hydroxyquinoline concentration is 0.001%-50. 0%, preferably 0.001%-10.0%, most preferably 0.05%-3. 0%.

Generally, in the composition of the present invention, the ratio (weight ratio) of S-ABA: 4-chloroindoleacetic acid (4-CI-IAA) or indolebutyric acid (IBA) is 1: 0.001-100, preferably 1 : 0.01- 50; In addition, S-ABA: 4-chloroindoleacetic acid (4-CI-IAA) or indolebutyric acid (IBA): The ratio (weight ratio) of naphthaleneacetic acid (NAA) is 1: 0. 001- 100: 0. 001- 100, preferably 1: 0. 01-

In addition, the plant growth regulating composition of the present invention may further contain various auxiliary agents. The adjuvants include: non-ionic surfactants, Tween, chitin, gelatin, sodium carboxymethyl cellulose, starch, and the like.

The plant growth regulating composition containing the natural abscisic acid of the present invention can be applied to various herbaceous plants, woody plants and shrubs at the seedling stage, the transplanting stage, the planting stage, and the cuttings and roots. The above woody plants include, for example, cereal crops, corn, wheat, rice, cotton, tobacco, flowers (including chrysanthemum, peony, rose red, impatiens, rose, camellia, Milan, magnolia, carnation, violet, lotus, etc.) , Ornamental plants, vegetables (including rapeseed, cucumber, pumpkin, watermelon, winter melon, celery, onion, cabbage, lettuce, tomato—, pepper, bell pepper, lettuce, cabbage, beet, radish, carrot, kidney bean, pea, soybean, Potatoes, etc.), and animal husbandry, Chinese herbs, lawns, etc. Greenhouse vegetables, rice, cotton, tobacco, etc. are preferred.

Woody plants such as Quercus, Birch, Willow, Elm, Maple, Pinaceae, Cypress, Tiliaceae, Waxaceae, Salixceae, Beech, Cedar, Euonymous, mn ^ Camphoraceae and other tree species, as well as economic forest apple, pear, peach, persimmon, walnut, chestnut, apricot, plum, grape, date tree, etc. Shrub species include: Amorpha fruticosa, Hippophae rhamnoides L., Populus euphratica, B. boxwood, Fig, B. eriophylla, Berberis, Euonymus, Ligustrum, Hongrui, Weigela, Lonicera, Elderberry, etc. Preferred are poplar, locust tree (Chinese locust, Robinia pseudoacacia), yellow carob orchid and the like.

The application dose can be varied within a relatively wide range and depends on the type of application (foliar application, application, root irrigation, seed dressing, injection, etc.), type of cultivated plant, growth period of the plant, climatic conditions, and other factors. In general, the composition according to the invention is applied at a dosage of from 0.01 to 2.50 kg of active ingredient per hectare. In general, the application dose of the plant growth regulating composition during application is not critical, and it is critical that the abscisic acid concentration during use is maintained at 1.0-. 500ppm.

When the plant growth regulating composition of the present invention is used, a conventional preparation form is used. They are processed, for example, into emulsifiable concentrates, sprayable or dilutable solutions, Dilute emulsion, wettable powder, soluble powder, powder, granule or microcapsule. Appropriate to the type of composition, application methods-such as spraying, misting, dusting, showering, wetting, root soaking, spraying, pouring or dipping cuttings. Make a choice based on the intended purpose and prevailing circumstances.

The formulations are prepared in a known manner, for example by mixing a plant growth regulator with the adjuvant, typically a homogeneous mixture of a solvent or a solid carrier and / or grinding. Surfactants can also be used in addition to prepare the formulation.

Examples of solvents and solid carriers are described in the second edition of Pesticide Dosage Processing Technology, edited by Liu Bulin (Chemical Industry Press, 1998).

Depending on the plant growth regulator to be processed, suitable surface-active compounds are nonionic, cationic and / or anionic surfactants and surfactant mixtures with good emulsifying, dispersing and wetting properties.

Examples of suitable anionic, cationic and / or nonionic surfactants are listed in the third edition of Pesticide Formulation Processing Technology, edited by Liu Bulin (Chemical Industry Press, 1998).

0% 至 99.9% Solid plant growth composition usually contains from 0.1% to 99.0% by weight, preferably from 0.1% to 95.0% by weight of the active ingredient, from 1.0% to 99.9% by weight 1% 至 25.0% 的 surf active agent or liquid processing aid and 0 to 25.0% by weight, preferably from 0.1% to 25.0% by weight.

A particularly preferred formulation composition is as follows:

(% = Percent by weight)

EC:

Active ingredient 1% to 90%, preferably 5% to 20%

Surfactant 1% to 30%, preferably 10% to 20%

Liquid carrier 5% to 94%, preferably 70% to 85%

ML

Active ingredient 0.1% to 10%, preferably 0.1% to 5%

Solid carrier 99.9% to 90%, preferably 99.9% to 99%

Suspension:

Active ingredient 5% to 75%, preferably 10% to 50%

94% to 24% water, preferably 88% to 30%

Surfactant 1% to 40%, preferably 2% to 30%

Wettable powder Active ingredient 0.5 to 90%, preferably 1 to 80% Surfactant 0.5 to 20%, preferably 1 to 15%

Liquid carrier 5% to 95%, preferably 15% to 90%

Granules:

Active ingredient 0.1% to 30%, preferably 0.1% to 15%

Solid carrier 99.5% to 70%, preferably 97% to 85%

The invention is illustrated by the following non-limiting examples

1. EC A) B) 0 D)

Active ingredients 5% 10% 25% 50%

Calcium dodecyl besylate 6% 8% 6% 8%

Polyethoxylated castor oil

(36 moles E0) 4%-4% 4%

Octylphenol polyethoxy

Compound (7 moles E0)-4%-2%

Cyclohexanone--10% 20%

Aromatic mixtures-C, ₂ 85% 78% 55% 15%

Any desired emulsion can be prepared by diluting the emulsifiable concentrate with water.

2. Wettable powder A) B) 0 D)

Active ingredients 5% 25% 50% 80%

Sodium lignosulfonate 4%-3%

Sodium lauryl sulfate 2% 3% ― 4%

Sodium diisobutylnaphthalenesulfonate 6% 5% 6%

Octylphenol polyethoxy

Compound (7-8 moles E0)-1% 2%-Highly dispersed silicic acid 1% 3% 5% 10%

Kaolin 88% 62% 35% 1

The active ingredients are thoroughly mixed with the auxiliaries, and the resulting mixture is ground in a suitable mill to obtain a wettable powder, which can be diluted with water to obtain a suspension of any desired concentration. 3. Coated granules A) B) C) Active component 0.1% 5% 15% Highly dispersed silicic acid 0.9% 2% 2% Inorganic carrier (Φ0.1-1) 99.0% 93% 83% Such as calcium carbonate Or silica

The active ingredient was dissolved in N-pyrrolidinone, and the solution was sprayed onto the carrier, and the solvent was removed by vacuum drying.

4. Coated granules A) B) C) Active component 0.1% 5% 15% Polyethylene glycol (MW200) 1.0% 2% 3% Highly dispersed silicic acid 0.9% 1% 2% Inorganic carrier (Φ0.1 -1 side) 98.0% 92% 80% such as calcium carbonate or silica

The finely-ground active ingredient was uniformly coated on a carrier wetted with polyethylene glycol in a mixer, thereby obtaining a dust-free powder.

5.Extrusion granules A) B) C) D)

Active ingredient 1% 3% 5% 15%

Sodium lignosulfonate 1.5% 2% 3% 4%

Carboxymethyl cellulose 1.4% 2% 2% 2%

Kaolin 97.0% 93% 90% 79%

The active ingredients are mixed and ground together with the adjuvant, the mixture is wetted with water, the above mixture is extruded, and then dried under air flow.

6. Powder A) B) 0

Active ingredient 0.1% 1% 5% Talc or chalk 39.9% 49% 35% Kaolin 60.0% 50% 60% Directly available powder is obtained by mixing the active ingredient with a carrier and grinding in a suitable mill.

7. Suspending agent A) B) C) D) Active component 3% 10% 25% 50% Ethylene glycol 5% 5% 5% 5% Nonylbenzene cool polyethoxy

Compound (15 moles E0)-1% 2%-Sodium lignosulfonate 3% 3% 4% 5% Carboxymethyl cellulose 1% 1% 1% 1%

37% formaldehyde aqueous solution 0.2% 0.2% 0.2% 0.2% silicone oil emulsion 0.8% 0.8% 0.8% 0.8% water 87% 79% 62% 38% The finely ground active substance is thoroughly mixed with the adjuvant. Suspensions are obtained in this way, and suspensions of any desired concentration can be prepared by dilution with water.

It is usually convenient to process each component separately and mix it by the user at the desired mixing ratio before use and apply it as a "tank mix".

Each component can be applied simultaneously or separately; when applied separately, the order of application usually does not affect its effect. When the plant growth regulating composition containing the natural abscisic acid of the present invention is used to treat a plant, it can be applied by spraying the entire plant, spraying seedlings or soaking roots, and soaking cuttings.

In general, it is critical when applying that the concentration of natural abscisic acid during application should be maintained at 1. 0-500 ppm _; preferably 1. 〇-lOOppm, most preferably 1. 0-50. Oppn

The active compounds used according to the invention can also be mixed with other active compounds, for example with known fungicides, pesticides and plant nutrients, thereby broadening the spectrum of action or preventing the development of resistance and treating various deficiencies. Vegetarian disease. In most cases, synergistic activity occurs, that is, the activity of the mixture exceeds the activity of the single component.

Examples of co-components in the mixture are as follows:

Neutral or acidic fungicides such as: carbendazim, thiabendazole, leaf withering, nongliling, skelin, sclerotia, chlorpyrimid, triazolone, azoxystrobin, metronidazole, paco Dexter, Diltiazone, Enazodone, Triazolol, Prozac, Zhiweiling, Dicarbendazim, Fenazol, Tefulin, Thiaconazole, Methionil, Chlorphene, Dimethrin Pine, seed dressing spirit, rice blast net, ethicillin, rice foot green, zinc substitute, zinc substitute, manganese zinc, formaldehyde, Bordeaux solution, copper sulfate, humic acid, mycotoxin 120, Gongzhuling Mycin, Actinomycin, Isoblasticin, Kasugamycin, Polyoxin, Pestocin, Griseofulvin, Jinggangmycin, (Agricultural) Streptomycin, Ningnanmycin, Chloramphenicol , Ribavirin, big green leaves, isatis root, houttuynia cordata, evergreen, andrographis paniculata, etc .; Plant nutrients such as trace element fertilizers such as trace element fertilizers containing boron, molybdenum, zinc, manganese, iron, copper, cobalt and other elements; and rare earth element fertilizers such as rare earth element fertilizers containing rhenium, lanthanum, cerium, praseodymium, neodymium and other elements .

But generally not mixed with alkaline substances.

Application Examples

Example 1: Use effect test of poplar transplanting period

Three to seven days before the transplantation of the poplar, the plant growth regulating composition of the present invention is used to perform a spray treatment on the poplar seedlings _{: the} poplar seedlings are measured for each of the indicators listed in Table 1 below.

Table 1.Effectiveness test during the transplanting period of seedlings (poplars)

The results show that after applying the plant growth regulating composition of the present invention, the root system of poplar tree seedlings is developed and grows robustly, which improves the drought and cold resistance of seedlings, and improves the survival rate of transplanting.

Example 2: Use effect test of Yellow Carnation Applying the plant growth regulating composition of the present invention to a spray treatment of the yellow carob tree 3-10 days before transplantation, or soaking the root for 3-10 minutes, the yellow carob tree is determined as listed in Table 2 below. Indicators.

Table 2 Effect test during the transplanting period of nursery stock

The results show that after applying the plant growth regulating composition of the present invention, the root system of C. angustifolia is developed and grows robustly, which improves the drought and cold resistance of seedlings, and improves the survival rate of transplanting.

Example 3: Use effect test of locust tree during transplanting period

After the locust tree is transplanted for 2 to 5 days, the plant growth regulating composition of the present invention is used to spray the locust tree seedlings once, or the roots are soaked for 3 to 10 minutes, and the indexes of the locust tree seedlings listed in Table 3 are measured. Table 3 Test of the use effect in the transplanting period of seedlings (locust trees)

The results show that after applying the plant growth regulating composition of the present invention, the roots of locust tree seedlings are developed and grow robustly, which improves the drought and cold resistance of seedlings, and improves the survival rate of transplanting.

Claims

Claim

1. A plant growth regulating composition which, in addition to a conventional inert processing aid, contains the following combinations as active ingredients: A) Natural abscisic acid and BM-indoleacetic acid or indolebutyric acid.

2. The plant growth regulating composition according to claim 1, further comprising naphthaleneacetic acid of component C).

The plant growth regulating composition according to claim 2, further comprising 8-hydroxyquinoline.

4. The plant growth regulating composition according to claim 1, wherein the weight-to-weight ratio of components A): B) is 1: 0.001-100.

The plant growth regulating composition according to claim 4, wherein the weight ratio of components A): B) is 1: 0.01-50.

The plant growth regulating composition according to claim 2, wherein the weight ratio of components A): B): C) is 1: 0.001-100: 0.001-lOOc

The mash growth regulating composition according to claim 6, wherein the weight ratio of components A): B): C) is 1: 0.01-50: 0.01-50.

8. Use of a plant growth regulating composition according to any one of claims 1 to 7 to treat plants.

9. Use according to claim 8, wherein the plants are herbs, woody plants and shrubs.

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