Summary of the invention
The object of the present invention is to provide a kind of leaf-protecting agent.
The active component of leaf-protecting agent provided by the invention mainly is made up of the raw material of following weight portion:
Oxine sulphate 1-5 part, polyacrylic acid potassium 5-10 part, NAA 0.01-0.1 part and wetting solvent 2-3 part.
Above-mentioned active component mainly is made up of the raw material of following weight portion:
2.5 parts of 3 parts in oxine sulphate, 7 parts of polyacrylic acid potassiums, 0.05 part of NAA and wetting solvents.
Above-mentioned leaf-protecting agent also comprises the water of 81-92 weight portion.
Above-mentioned leaf-protecting agent can be made up of the raw material of following weight portion:
Oxine sulphate 1-5 part, polyacrylic acid potassium 5-10 part, NAA 0.01-0.1 part, wetting solvent 2-3 part and water 81-92 part.
Specifically, above-mentioned leaf-protecting agent can be made up of the raw material of following weight portion:
87.45 parts in 3 parts in oxine sulphate, 7 parts of polyacrylic acid potassiums, 0.05 part of NAA, 2.5 parts of wetting solvents and water.
Arbitrary above-mentioned wetting solvent can be a sodium metnylene bis-naphthalene sulfonate; Arbitrary above-mentioned oxine sulphate is the oxine potassium sulphate; The number-average molecular weight of arbitrary above-mentioned polyacrylic acid potassium is 2,000,000-4,000,000.
The preparation method of the leaf-protecting agent that another object of the present invention is to provide above-mentioned.
The preparation method of leaf-protecting agent provided by the invention may further comprise the steps:
1) 1/2nd water with said weight portion is heated to 78-82 ℃, adds the polyacrylic acid potassium of said weight portion, makes it to dissolve fully, be cooled to room temperature after, add the oxine sulphate of said weight portion, obtain mixed liquor;
2) behind the NAA and the dissolving of wetting solvent of water with residuals weight part, join in the mixed liquor of step 1), stir, obtain described leaf-protecting agent said weight portion.
Above-mentioned steps 1) in, the temperature of water heating is 80 ℃.
Above-mentioned wetting solvent is a sodium metnylene bis-naphthalene sulfonate; Above-mentioned oxine sulphate is the oxine potassium sulphate; The number-average molecular weight of above-mentioned polyacrylic acid potassium is 2,000,000-4,000,000.
Oxine sulphate: bactericide, the disease-resistant bacterium of enhancement of plant, virus capable are regulated stomatal conductance, cold-resistant, the drought-resistant ability of enhancement of plant.
Polyacrylic acid potassium: form the network structure protective film at plant leaf surface, water conservation and reduce its moisture transpiration, reduce photosynthetic rate.
NAA: plant growth regulator, promote root growth, improve the ability that seedling absorbs nutrient and trace element.Dispersing agent NNO (sodium metnylene bis-naphthalene sulfonate): wetting solvent, make active ingredient fully disperse to launch at plant leaf surface, be beneficial to absorb.
The object of the invention is intended to the common coordinative role through several kinds of chemical agents; Form a kind of mixing material, after the transplanting plant was sprayed, the part of related substances and plant leaf blade, stem were had an effect; Form a kind of diaphragm of compound molecule structure; To reduce its rising dehydration, strengthen the defence resistivity of plant to sick worm and adverse circumstance, prevent that simultaneously functional leaf Yin Shengli from consuming coming off of causing; Another part by plant absorbing after, can promote root of hair and root growth, strengthen absorbing capacity to nutritive element.Particularly carrier-free transplanting plant seedlings can effectively improve transplanting survival rate and shorten transplanting seedling time.
The experiment proof: compare with the seedbed seedling, 3 processing of stomatal conductance of leaving the bed seedling descend 83.3%, 66.7% and 29.1% respectively; Photosynthetic rate descends 50.7%, 16.0% and 9.5% respectively, and transpiration rate descends 76.9%, 59.3% and 26.5% respectively.The result points out that leaf-protecting agent has significant regulating effect to stomatal conductance, photosynthetic rate and the transpiration rate of cotton leaf, and when cotton was exsomatized or meet with arid, the foliage-spray leaf-protecting agent had fresh-keeping and effect that take precautions against drought.
After adopting leaf-protecting agent provided by the invention, single transplantation of seedlings average survival can improve more than 60%, and single transplantation of seedlings survival rate is up to more than 96.4%, and seedling-slowing stage shortens 5-7 days.This leaf-protecting agent is nontoxic, harmless, noresidue.
Embodiment
Below in conjunction with specific embodiment the present invention is described further, but the present invention is not limited to following examples.
Among the following embodiment,, be conventional method like no specified otherwise.
Among the following embodiment, said percentage composition is the quality percentage composition like no specified otherwise.
The oxine potassium sulphate is available from the friendly chemical industry in Wuxi three Co., Ltd.
Polyacrylic acid potassium (low crosslinking degree, number-average molecular weight 2,000,000-4,000,000) is available from Henan, Zhengzhou China auxiliary agent Co., Ltd.
NAA is available from the chemical experimental factory, Anyang.
Dispersing agent NNO (sodium metnylene bis-naphthalene sulfonate) is available from Anyang dicyclo auxiliary agent Co., Ltd.
The preparation of embodiment 1, leaf-protecting agent
One, each amounts of components of leaf-protecting agent
Oxine potassium sulphate 3g, polyacrylic acid potassium 7g, NAA 0.05g, dispersing agent NNO 2.5g and water 87.45g.
Two, preparation leaf-protecting agent
1) 1/2nd water with above-mentioned weight portion is heated to 80 ℃, adds the polyacrylic acid potassium of 7g, makes it to dissolve fully, be cooled to room temperature after, add oxine potassium sulphate 3g, obtain mixed liquor;
2) join in the mixed liquor of step 1) after with the water of residuals weight part, stir, obtain leaf-protecting agent of the present invention the NAA of 0.05g and the dissolving of 2.5g dispersing agent NNO.
The preparation of embodiment 2, leaf-protecting agent
One, each amounts of components of leaf-protecting agent
Oxine potassium sulphate 1g, polyacrylic acid potassium 5g, NAA 0.01g, dispersing agent NNO 2g and water 84g.
Two, preparation leaf-protecting agent
1) water with 4 above-mentioned weight portions 1/2nd is heated to 80 ℃, adds the polyacrylic acid potassium of 5g, makes it to dissolve fully, be cooled to room temperature after, add oxine potassium sulphate 1g, obtain mixed liquor;
2) join in the mixed liquor of step 1) after with the water of residuals weight part, stir, obtain leaf-protecting agent of the present invention the NAA of 0.01g and the dissolving of 2g dispersing agent NNO.
The preparation of embodiment 3, leaf-protecting agent
One, each amounts of components of leaf-protecting agent
Oxine potassium sulphate 5g, polyacrylic acid potassium 10g, NAA 0.1g, dispersing agent NNO 3g and water 92g.
Two, preparation leaf-protecting agent
1) water with above-mentioned weight portion 1/2nd is heated to 80 ℃, adds the polyacrylic acid potassium of 10g, makes it to dissolve fully, be cooled to room temperature after, add oxine potassium sulphate 5g, obtain mixed liquor;
2) join in the mixed liquor of step 1) after with the water of residuals weight part, stir, obtain leaf-protecting agent of the present invention the NAA of 0.1g and the dissolving of 3g dispersing agent NNO.
Embodiment 4, Function detection
One, the statistics of stomatal conductance, photosynthetic and transpiration rate
1, the experiment step
Used leaf-protecting agent is the leaf-protecting agent of embodiment 1 preparation.Used plant variety is middle cotton institute 45 (available from a science and technology trading company of The Chinese Academy of Agriculture Science and Technologys Cotton Research Institute); Adopt the cave microcell to grow seedlings; (33.0cm * 33.0cm * 11cm), sowing yardstick specification (line-spacing * grain distance): 8cm * 2.67cm, seedling medium is 1: 1 with clean river sand volume ratio to the cave specification.After treating that cotton grows 2~3 true leaves,, be divided into 3 groups, spray the leaf-protecting agent of following three kinds of concentration respectively: clear water contrast, 15 times of liquid of dilution, 5 times of liquid of dilution according to 9 consistent nursery ponds of growth of completely random principles of selected.Spray with watering can, with even moistening being as the criterion of whole blade.Cotton seedling (from a bed seedling) is transplanted in cotton seedling of every group of picked at random 15 strain and 15 strains, and in spraying back 1h, 2h, 3h, 24h, 25h, 72h and 73h, the licor6400 with U.S. genome company measures photosynthesis, blade transpiration and stomatal conductance to cotton leaf respectively.Carry out the Repeated Measurement Data variance analysis method to data analysis with dps2005 at last.
2, result
1) different disposal is to the influence of cotton leaf stomatal conductance
Seedbed seedling result: complexity and the blade of stomatal conductance reflection gas through pore is to the ability of regulation and control of moisture loss.Result of the test shows (table 1); The leaf-protecting agent that sprays variable concentrations in the seedbed does not have each test of remarkable influence (p=0.5414) to present the periodicity diurnal variation because climatic environment such as influences such as temperature, illumination are passed in time between handling to the stomatal conductance of cotton seedling leaf; Significant difference is used not significant relation with leaf-protecting agent.But the trend that reduces is gradually arranged generally.
Leave a bed seedling result: after the matrix cotton plays transplantation of seedlings; Because the destruction of part root structure; With the change of root system environment temperature, cause stomatal conductance significantly to reduce, result of the test shows (table 1); In 3 processing (clear water contrast, 15 times of liquid of dilution, 5 times of liquid of dilution) from the bed seedling respectively than having reduced by 83.3%, 66.7% and 29.1% on the seedbed, this mainly is the cause that has sprayed leaf-protecting agent.Find out that from table 1 the leaf-protecting agent different disposal does not have significant difference to leaf stomatal conductance regulating action effect remarkable (P=0.0218) between the duplicate measurements of each time.Better to dilute 5 times of liquid in 3 processing, stomatal conductance reaches 0.039molm
-2s
-1, the stomatal conductance of 15 times of liquid of dilution is 0.027molm
-2s
-1Stomatal conductance is very high; Then reduce gradually with the prolongation of transplanting the lifting time, cotton seedling is after spraying the leaf-protecting agent lifting, and stomatal conductance significantly reduces; But after spraying leaf-protecting agent; Stomatal conductance underspeeds and significantly slows down, and regulates stomatal conductance in the past at 24h and plays a role clearly, and regulating action slowly weakens behind the 24h.Basically approach the clear water control treatment to the end, but still be higher than the clear water contrast.
Table 1 seedbed seedling and from bed seedling leaf-protecting agent different disposal cotton leaf stomatal conductance unit a: mol.m
-2s
-1
2) different disposal is to the influence of cotton leaf photosynthesis rate
Seedbed seedling result: the diurnal variation trend cotton of photosynthetic rate has single peak type that bimodal pattern is also arranged, and in September, temperature and illumination all can not cause the lunch break phenomenon of cotton, and the photosynthesis of this moment is single peak type.Find out that from table 2 spraying leaf-protecting agent does not have appreciable impact (P=0.5407>0.05) to seedbed cotton photosynthesis, each time point photosynthesis significant difference, the interaction of measuring between repetition and processing is extremely remarkable.See that from 3 variable concentrations processing the clear water contrast is 11.676 μ molCO
2M
-2s
-1, diluting 15 times of liquid is 11.057 μ molCO
2M
-2s
-1, diluting 5 times of liquid is 9.811 μ molCO
2M
-2s
-1, can find out that on the seedbed, spraying leaf-protecting agent has the photosynthetic presentation of slight inhibition, but not remarkable.
Table 2 seedbed seedling and from a bed seedling cotton photosynthesis rate comparative unit: μ molCO
2.m
-2s
-1
Leave a bed seedling result: cotton seedling lifting is behind bed, and photosynthesis significantly descends, because the destruction of part root structure and root water uptake ability fail, photosynthetic rate sprays 3 processing of leaf-protecting agent and reduced 50.7%16.0% and 9.5% than seedbed seedling respectively.This mainly is the cause that has sprayed leaf-protecting agent, and result of the test shows that the leaf-protecting agent different disposal does not have significant difference to leaf photosynthesis rate adaptation action effect remarkable (P=0.0134) between each time duplicate measurements.Better with the effect of diluting 15 times of liquid in 3 processing of leaf-protecting agent, photosynthetic rate reaches 9.28 μ molm
-2s
-1That dilute 5 times of liquid is 8.88 μ molm
-2s
-1Photosynthetic rate is very high, and the intensity that reduces gradually with the prolongation of transplanting the lifting time differs, and the photosynthetic rate straight line that no leaf-protecting agent is handled descends, and what spray then keeps a state more stably, slowly decline, and wherein 15 times of stoste effects are best.
3) different disposal is to the influence of cotton leaf transpiration rate
Seedbed seedling result: transpiration is the important vital movement process of plant, and transpiration falls sharply and means that the cotton normal activities is suppressed, because transpiration water consumption often causes water deficit; Suppress the cotton normal activities; On the one hand, under adverse environmental factor, too high transpiration rate; Cause the blade dehydration too fast, the adverse circumstance adaptive capacity is poor.Cotton foliage-spray leaf-protecting agent has regulating effect preferably.Find out from table 3, on the seedling of seedbed, spray leaf-protecting agent, different disposal is to the difference of transpiration rate influence not significantly (p=0.7405>0.05), each time period since ambient lighting, temperature, humidity etc. influence significant difference, little with the relation that leaf-protecting agent is handled.
Table 3 seedbed seedling and from the bed seedling spray leaf-protecting agent different disposal cotton transpiration rate unit: mmol.m
-2s
-1
Leave a bed seedling result: cotton seedling lifting is behind bed, and transpiration significantly descends, because the destruction of part root structure and root water uptake ability fail.Result of the test shows (table 3), and transpiration rate sprays 3 processing of leaf-protecting agent and reduces by 76.9%, 59.3% and 26.5% than seedbed seedling respectively.Leaf-protecting agent regulating action significant difference (p=0.0212<0.05), this mainly is the cause that has sprayed leaf-protecting agent.Can find out that from table 3 the leaf-protecting agent processing that sprays 5 times of liquid of dilution reduces by 26.5% to blade transpiration rate regulating action effect than the seedbed seedling, mainly be because the leaf-protecting agent of 5 times of liquid concentration of dilution causes greatly the rising inhibitory action on the seedbed.The effect optium concentration can make transpiration rate maintain 0.891mmolm about 15 times of liquid concentration of dilution
-2s
-1Significantly reduce owing to respectively handle transpiration behind the lifting, do not have significant difference between the duplicate measurements of each time.
Two, leaf-protecting agent is to transplanting the experiment of cotton shoot survival percent and seedling-slowing stage
1 experimental procedure:
Used kind is a middle cotton 45, adopts the seedling dish to grow seedlings, and seedling dish specification is that (33.0cm * 33.0cm * 11cm), the cotton seed in a cave is established 4 processing altogether, is respectively 5 times of liquid of dilution, dilutes 10 times of liquid, dilutes the contrast of 20 times of liquid and clear water.When treating that seedling dish cotton grows 2-3 sheet true leaf, blade is moistening is advisable to spray, and according to 9 consistent seedling dishes of growth of completely random principles of selected, is divided into 3 groups; Transplanting the leaf-protecting agent that sprays variable concentrations the previous day respectively, with the moistening degree of being of blade, clear water contrast spray is with clear water, also with the moistening degree of being of blade; Then each is handled and transplant the cotton seedling of 15 strains, in spraying back 1d, 3d; 5d, 7d, 14d observes record to cotton seedling.
2 experimental results
(1) spray 1d after observed result following: each is handled plant and poisoning do not occurred, but it is shinny to dilute the strain leaf look of 5 times of liquid, and greening has slight poisoning.
(2) spray 3d after observed result following: each handles plant does not all have poisoning to take place.It is normal green that the plant leaf look of 5 times of liquid of dilution recovers; 1 seedling death phenomenon appears in the clear water contrast.Each treatment region is best with the cotton seedling growing way of diluting 10 times of liquid.
(3) spray 5d after observed result be: the dilution 10 times of liquid plant grow young leaves, leaf look greening; The whole growing way of the plant of 20 times of liquid of dilution is pretty good, but does not have young leaves; The plant of 5 times of liquid of dilution does not have young leaves yet; The cotton seedling growing way in clear water check plot is executed the leaf-protecting agent district not as all, occurs 2 seedling death phenomenons again.
(4) spray 7d after observed result be: young leaves appears in the plant of 5 times of liquid of dilution, but 1 seedling death phenomenon occurs; The appearance 1 strain wilting situation of 10 times of liquid of dilution; The also appearance 1 strain seedling death phenomenon of 20 times of liquid of dilution; Part cotton seedling in clear water check plot is also in slow seedling process.
(5) spray behind the 14d observed result such as table 4 time:
The statistics of table 4 after cotton two weeks of transplantation of seedlings
Handle |
Survival rate (%) |
Slow seedling fate |
Dilute 5 times of liquid |
89.3% |
3d |
Dilute 10 times of liquid |
98.6% |
2d |
Dilute 20 times of liquid |
87.3% |
4d |
The clear water check plot |
67.3% |
9d |
Can find out by last table; Transplanted seedling is sprayed after leaf-protecting agent handles (contrast water spray), and done the comparative trial of variable concentrations, the result shows; 3 concentration of leaf-protecting agent are handled (3 different extension rates), and each is handled the transplanting survival rate of seedling and all compares according to being significantly improved.But the variable concentrations treatment effect also is not quite similar, and leaf-protecting agent is to dilute 10 times for best.In a last experiment; Can increase stomatal resistance though dilute 5 times, reduce moisture evaporation, survival rate is on the contrary than at the bottom of 10 times; Reason possibly be because soup is oversize action time; The undue moisture evaporation that suppresses can make normal light of plant and effect and other physiological activity be affected on the contrary, causes survival rate to descend to some extent.Suggestion is used leaf-protecting agent doubly to spray on the blade face with dilution 10-15 and is advisable.
Each slow seedling fate of handling seedling is 2-4 days, has shortened 5-7 days (slow seedling fate just be meant transplant cotton seedling be transplanted to the fate that the land for growing field crops plant restore normal growth and experienced from the seedling dish) than clear water contrast.
Embodiment 2 carries out Function detection with the leaf-protecting agent that embodiment 3 prepares according to the method for embodiment 4, and the result of its stomatal conductance, photosynthesis rate, transpiration rate, survival rate and seedling-slowing stage is consistent with embodiment 1.