WO2003090534A1

WO2003090534A1 - Plan growth regulating composition for cold-resistance

Info

Publication number: WO2003090534A1
Application number: PCT/CN2002/000279
Authority: WO
Inventors: Xianguo Fan; Hong Tan; Hubao Chen; Daxiang Li
Original assignee: Sichuan Lomon Bio Technology Co., Ltd.; Chengdu Institute Of Biology Chinese Academy Of Sciences
Priority date: 2002-04-23
Filing date: 2002-04-23
Publication date: 2003-11-06
Also published as: CN1379981A; CN1209017C

Abstract

The present invention relates to a plant growth regulating composition for cold-resistance, comprising as active components A) a natural type abscisic acid, B) nicotinamide and optional. C) at least one plant growth regulator selected from the group consisting of jasmonic acid, methyl jasmonate or brassinolide, in addition to general inert auxiliaries.

Description

Technical field of plant growth regulating composition with cold resistance

The invention relates to a plant growth regulating composition containing natural abscisic acid, which has a cold-resistant effect, and belongs to AQlN37 / 06.

Background technique

Natural abscisic acid (S-ABA), common name of pesticide: S-actin. Molecular formula: C _1S > 0 ₄ , chemical name is: 5- (1 '-hydroxy-2', 6 ', 6' -trimethyl-4 '-oxo-2' -cyclohexene- -yl)- 3-Methyl-2-cis-4-trans-pentadienoic acid, the chemical structure is as follows

Chemical structure of S-ABA.

In 1963, abscisic acid was added for the first time from Addicott et al. For more than three decades, scientists from various countries have conducted extensive and in-depth studies on the role of plant physiological activities. Studies have shown that abscisic acid is a plant's "stress-resistance-inducing factor" and "balanced growth factor". It can start the plant's stress-resistance gene expression, activate the plant's endogenous immune system, and induce the plant to biotic and abiotic stress (severe drought, Low temperature, saline-alkali, diseases and insect pests) produce resistance, improve the plant's ability to resist drought, cold, disease and saline-alkali, can be widely used in agriculture, forestry, animal husbandry, landscaping, ecological vegetation restoration and other fields.

Although S-ABA can induce many low temperature-induced proteins, from the expression of low temperature-induced genes, there may be information other than ABA that can induce low temperature stress. Therefore, S-ABA (0.5-500. 0ppm) alone will not be effective for all crops, and the depth of low temperature resistance is limited.

Nicotinamide is a prosthetic group of Coenzyme I, which is beneficial to promote the growth of seedlings at low temperature. Nicotinamide-containing growth regulator compositions such as CN1022413A contain at least one choline salt and nicotinamide for regulating plant growth. CN1045870A has publicly disclosed a kind of baolingzhikuling: nicotinamide 1-10% (weight ratio), 2, 4-D 0. 1-2%, kinetin 5-25%, and auxin 15-50%, 7-40% chlormequat, 2-20% morphogen, 30-60% other anti-disease substances, 5-10% additives, and the sum of each component is 100% by weight. CN1172093A discloses a vegetable and seedling crop growth promoter, which includes urea, anhydrous ferrous sulfate, vitamin B, and nicotinamide. The results of research and experiment by the present inventors have found that the combination of S-ABA and nicotinamide can improve the cold resistance of crops, and exhibits a wider range of cold resistance than single use.

Summary of the Invention

The object of the present invention is to provide a plant growth regulating composition containing natural abscisic acid with cold resistance, which is characterized in that the composition includes A) natural abscisic acid and B) nicotinamide.

The plant growth regulating composition of the present invention may further contain component C) at least one plant growth regulating substance selected from the group consisting of glorinic acid, methyl jasmonate or brassinolide.

Surprisingly, the composition of S-ABA, nicotinamide, and other plant growth-regulating substances added to the present invention on the growth-regulating activity of crops exceeded the expected additive effect, mainly on the one hand, improving each The active range of these ingredients; on the other hand, the application concentration of natural abscisic acid and other plant growth regulators is reduced, and the plant growth regulator combination also obtains a good level of plant growth regulation when the single compound is lower than the effective concentration.

Various plant growth regulators in the present invention: nicotinamide, jasmonic acid, methyl jasmonate (PDJ), brassinolide (BR, TS303), etc. are all known and described in, for example, "Plant Growth Regulation Agent Xie Jiuying, Chief Editor, Hong Kong Chinese Science and Technology Press, 1992.5; New Handbook of Pesticides, Ministry of Agriculture Pesticide Certification Institute, Agricultural Press, 1989; New Handbook of Pesticides (Continuation), Ministry of Agriculture Pesticide Testing Institute, Agricultural Publishing Press, 1998, etc.

Although S-ABA can induce information on low temperature stress, S-ABA (0.5-500. Oppm) alone is not effective for all crops, and the depth of resistance to low temperature is limited. Nicotinamide is a prosthetic group of Coenzyme I, which is beneficial to promote the growth of seedlings at low temperatures. In the present invention, mixing S-ABA with nicotinamide has a complementary effect, and in particular, it exhibits a mutual promotion effect on cold resistance, thereby increasing yield and improving quality.

In general, the S-ABA use concentration in the composition of the present invention is 0.5-500.0 ppm, preferably 0.5-100.0 ppm, more preferably 1.0-30.0 ppm; the nicotinamide use concentration is 0.01-100 ppm, preferably 0.1-50% m, more preferably 0.1-5 ppm; methyl jasmonate (PDJ) or jasmonic acid is used at a concentration of 0.5-500.0 ppm, preferably 0.5-100.0 ppm, more preferably 1.0-50.0 ppm; brassinolide (BR Or TS303) using a concentration of 0.001-100. Oppm, preferably 0.001 10. Oppm, more preferably 0.001-1.0ppm _o

Generally, in the composition of the present invention, the ratio (weight ratio) of S-ABA: nicotinamide is 1: 0.001-100, preferably 1: 0.01-10; in addition, S-ABA: nicotinamide: jasmonic acid (methyl ester) and / Or the ratio (weight ratio) of brassinolide is 1: 0.001-100: 0.00001-100, preferably 1: 0.01-10: 0.00001-10. In addition, the plant growth regulating composition of the present invention may further contain a synergist. The synergist is at least one of the following components: sucrose, sucrose chelated calcium, vitamins, amino acids, organic fertilizers, nutritional elements, certain mineral elements, citric acid, CaCL, and the like.

The abscisic acid-containing plant growth regulating composition of the present invention can be applied to cereal crops, corn, wheat, wild rice, cotton, tobacco, flowers (including chrysanthemum, paeonia lactiflora, string red, impatiens, rose, camellia, Milan, magnolia, Dianthus, violet, lotus, etc.), fruit trees (including apples, mandarins, pears, grapes, bananas, pineapples, dates, persimmons, citrus, grapefruit, lemon, strawberry, peach, apricot, plum, cherry, walnut, chestnut, litchi, Papaya, mango, sugar cane, etc.), ornamental plants, vegetables (including canola, cucumber, pumpkin, watermelon, winter melon, celery, onion, cabbage, eggplant, tomato, pepper, sweet pepper, lettuce, cabbage, beet, radish, carrot, (Beans, peas, soybeans, potatoes, etc.) as well as forages, Chinese herbs, lawns, etc. Greenhouse vegetables, rice, cotton, tobacco, etc. are preferred.

The application dose can be varied within a relatively wide range and depends on the type of application (foliar spray, application, root irrigation, seed dressing, injection, etc.), the type of cultivated plant, the growing period of the plant, climatic conditions and other factors. In general, the composition according to the invention is applied at a dosage of from 0.01 to 2.50 kg of active ingredient / ha. In general, the application dose of the plant growth regulating composition during application is not critical, it is critical that the abscisic acid concentration during use should be maintained at 0.5 to 500 ppra.

When the plant growth regulating composition of the present invention is used, a conventional preparation form is used. They are used in a conventional manner and are usually used in formulation processing techniques ^; adjuvants used are processed into, for example, emulsifiable concentrates, directly sprayable or dilutable solutions, dilutable emulsions, wettable powders, soluble powders, powders, granules or micro Capsules. Appropriate for the type of composition: Application method-such as spraying, misting, dusting, moisturizing, spraying or pouring, depending on the intended purpose and prevailing environment.

Formulations are prepared in a known manner, for example, by mixing a plant growth regulator with the adjuvant, typically a homogeneous mixture of a solvent or a solid carrier, and / or grinding. Surfactants can also be used in addition to prepare the formulation.

Examples of solvents and solid carriers are described in the second edition of Pesticide Dosage Processing Technology, edited by Liu Yilin (Chemical Industry Press, 1998).

Depending on the plant growth regulator to be processed, suitable surface-active compounds are non-ionic, cationic, anionic and / or amphoteric surfactants and surfactant mixtures with good emulsifying, dispersing and wetting properties.

Examples of suitable non-ionic, cationic, anionic and / or amphoteric surfactants are listed in the third edition of "Agrochemical Processing Technology" edited by Liu Bulin (Chemical Industry Press, 1998). The plant growth regulating composition usually contains from 0.1% to 99.0% by weight, preferably from 0.1% to 95.0% by weight of natural abscisic acid and nicotinamide, and component C, by weight 1. 0% 至 99.9% solid or liquid processing aids and 0 to 25.0% by weight, preferably from 0.1% to 25.0% by weight of the surfactant.

A particularly preferred formulation composition is as follows:

(% = Percent by weight)

EC:

Active ingredient 1% to 90%, preferably 5% to 20%

Surfactant 1% to 30%, preferably 10% to 20%

5% to 94%, preferably 70% to 85%

Active ingredient 0.1% to 10%, preferably 0.1% to 5%

Solid carrier 99.9% to 90%, preferably 99.9% to 99%

Suspension:

Active ingredient 5% to 75%, preferably 10% to 50%

94% to 24% water, preferably 88% to 30 ° / ₀

Surfactant 1 ° / ₀ to 40%, preferably 2% to 30%

Wettable powder

Active ingredient 0.5 to 90%, preferably 1 to 80%

Surfactant 0.5% to 20%, preferably 1% to 15 ° / ₀

Liquid carrier 5% to 95%, preferably 15% to 90%

Granules:

Active ingredient 0.1% to 30%, preferably 0.1% to 15%

Solid carrier 99.5% to 70%, preferably 97% to 85%

The invention is illustrated by the following non-limiting examples:

1. EC A) B) 0 D)

Active ingredients 5% 10% 25% 50%

Calcium dodecyl besylate 6% 8% 6% 8%

Polyethoxylated castor oil (36 moles E0) 4% 4% 4%

Octylphenol polyethoxy

Compound (7 moles E0) 4% 2%

Cyclohexanone 10% 20%

Aromatic mixture G, -C ₁₂ 85% 78% 55% 15%

Any desired emulsion can be prepared by diluting the emulsifiable concentrate with water-

2. Wettable powder A) B) 0 D)

Active ingredients 5% 25% 50% 80%

Sodium lignosulfonate 4% 3%

Sodium lauryl sulfate 2% 3% 4%

Sodium diisobutylnaphthalenesulfonate 6% 5% 6%

Octylphenol polyethoxy

Compound (7-8 moles E0) 1% 2%

Highly dispersed silicic acid 1% 3% 5% 10%

Kaolin 88% 62% 35%

The active ingredients are thoroughly mixed with the adjuvant, and the resulting mixture is ground in a suitable mill to obtain a wettable powder, which can be diluted with water to obtain a suspension of any desired concentration.

3. Coated granules A) B) C) Active component 0.1% 5% 15%

Highly dispersed silicic acid 0.9% 2% 2% Inorganic carrier (Φ 0. 1-1) 99.0% 93% 83% Such as calcium carbonate or silica

The active ingredient was dissolved in N-pyrrolidone, and the solution was sprayed onto the carrier, and the solvent was removed by vacuum drying.

4. Coated granules A) B) C) Active component 0.1% 5% 15% Polyethylene glycol (MW200) 1. 0% 2% 3%.

Highly dispersed silicic acid 0, 9% 1% 2% Inorganic carrier (Φ 0.1-lmm) 98.0% 92% 80% Such as calcium carbonate or silica

The finely-ground active ingredient was uniformly coated on a carrier wetted with polyethylene glycol in a mixer, thereby obtaining a dust-free powder.

5.Extrusion granules A) B) 0 D)

Active ingredient 1% 3% 5% 15%

Sodium lignosulfonate 1.5% 2% 3% 4%

Carboxymethyl cellulose 1.4% 2% 2% 2%

Kaolin 97.0% 93% 90% 79%

The active ingredients are mixed and ground together with the adjuvant, the mixture is wetted with water, the above mixture is extruded, and then dried under air flow.

6. Powder A) B) 0

Active ingredient 0.1% 1% 5% Talc or chalk 39.9% 49% 35% Kaolin 60. 0% 50% 60% Directly available powder by mixing the active ingredient with a carrier and grinding in a suitable mill obtain.

7. Suspending agent A) B) 0 D) Active ingredient 3% 10% 25% 50% ethylene glycol 5% 5% 5% 5% nonylphenol polyethyl

Compound (15 moles E0)-1% 2%-Sodium lignosulfonate 3% 3% 4% 5% Carboxymethyl cellulose 1% 1% 1% 1%

37% formaldehyde aqueous solution 0.2% 0.2% 0.2% 0.2% silicone oil emulsion 0.8% 0.8% 0.8% 0.8% water 87% 79% 62% 38% The finely ground active substance is thoroughly mixed with the adjuvant. Suspensions are obtained in this way, and suspensions of any desired concentration can be prepared by dilution with water.

It is usually convenient to separately process abscisic acid and nicotinamide and component C). It is mixed in a mixing ratio and applied in a "barrel mixing" manner.

Each component can be applied simultaneously or separately; when applied separately, the order of application usually does not affect its effect. By using the plant growth regulating composition containing the abscisic acid of the present invention to treat plants, foliar spraying, leaching or irrigation can be applied at the seedling stage, seedling stage, 130 days before encountering low temperature, preferably 1-10 days.

In general, it is critical when applying that the concentration of natural abscisic acid during application should be maintained at 0.5-500ppm, preferably

0.5-100 ppm _; most preferably 1.0-30, 0 ppm.

The active compounds used according to the invention can also be mixed with other active compounds, for example with known fungicides, pesticides and plant nutrients, thereby broadening the spectrum of action or preventing the development of resistance and treating various deficiencies. Vegetarian disease. In most cases, synergistic activity occurs, that is, the activity of the mixture exceeds the activity of the single component.

Examples of co-components in the mixture are as follows:

Neutral or acidic fungicides such as: carbendazim, thiabendazole, leaf withering, nongliling, skelin, sclerotia, chlorpyrimid, triazolone, azoxystrobin, metronidazole, paco Dexter, Diltiazone, Enazodone, Triazolol, Prozac, Zhiweiling, Dicarbendazim, Fenazol, Tefulin, Thiaconazole, Methionil, Chlorphene, Dimethrin Pine, seed dressing spirit, rice blast net, ethicillin, rice foot green, zinc substitute, zinc substitute, manganese zinc, formaldehyde, Bordeaux solution, copper sulfate, humic acid, mycotoxin 120, Gongzhuling Amycin, actinomycin, isorexin, kasugamycin, polyoxin, blasticidin, griseofulvin, Jinggangmycin, (agricultural) streptomycin, ningnanmycin, chloramphenicol , Ribavirin, big green leaves, isatis root, houttuynia cordata, evergreen, andrographis paniculata, etc .;

Plant nutrients such as trace element fertilizers such as trace element fertilizers containing boron, molybdenum, zinc, manganese, iron, copper, cobalt and other elements; and rare earth element fertilizers such as rare earth element fertilizers containing rhenium, lanthanum, cerium, praseodymium, neodymium and other elements .

But generally not mixed with degrading substances.

Application Examples

Example 1: Test of cold resistance on cucumber

At the seedling stage, seedling stage, 1-7 days before low temperature, or during low temperature, the plant growth regulating composition of the present invention is used to spray the cucumber seedlings one or more times, and the cucumber plants are determined as listed in Table 1 below. Indicators and record the final output. . Table 1: Cold resistance test on cucumber

The results show that after applying the plant growth-regulating composition of the present invention, the plant has a developed root system and a thickened stem, especially in the depth of cold resistance, that is, its cold resistance is stronger than that of S-ABA single agent (100. Oppni), especially at low temperature Lower seedlings can still get faster growth. The final increase in output was 17. 6-21. 5%.

Example 2: Cold resistance test on pepper

At the seedling stage, seedling stage, and adult stage of pepper, 1-7 days before the low temperature, or during low temperature, the plant growth regulating composition of the present invention is used to spray the pepper seedlings one or more times, and the pepper plants are measured as follows: The indicators listed in 2 and record the final output. Table 2 Cold resistance test on pepper

The results show that after applying the plant growth regulating composition of the present invention, the plant roots are developed and the stems are thickened, especially in the low-temperature resistance depth, that is, the cold resistance is stronger than S-ABA single agent (100. Oppm), especially at low temperature Lower seedlings can still get faster growth. The final increase was 17. 6-28. 4%.

Example 3: Cold resistance test on bananas

At the seedling stage of bananas, when it is exposed to low temperatures for 17 days, or during low temperatures, the plant growth regulating composition of the present invention is applied to the banana seedlings for one or more spray treatments, and the indicators of the banana plants are determined as shown in Table 3 below. And record the final output. Table 3 Test of cold resistance on bananas

The results show that after applying the plant growth regulating composition of the present invention, the plant roots are developed and the stems are thickened, especially in the depth of low temperature resistance, that is, the cold resistance is stronger than S-ABA single agent (100. Oppm), especially at low temperature Lower seedlings can still get faster growth. The final increase in output reached '26. 3-30. 8%.

Claims

Claim

1. A plant growth regulating composition which, in addition to a conventional inert processing aid, contains the following combinations as active ingredients: A) natural abscisic acid and B) nicotinamide.

The plant growth regulating composition according to claim 1, further comprising component C) at least one plant growth substance selected from the group consisting of jasmonic acid, methyl jasmonate or brassinolide.

The plant growth regulating composition according to claim 2, wherein the brassinolide is TS303.

The plant growth regulating composition according to claim 1, wherein the weight ratio of components A): B) is 1: 0.00 to 100.

The plant growth regulating composition according to claim 4, wherein the weight ratio of components A): B) is 1: 0.01-10.

The plant growth regulating composition according to claim 2, wherein the weight ratio of components A): B): C) is 1: 0.001-100: 0.00001-100.

The plant growth regulating composition according to claim 6, wherein the weight ratio of components A): B): C) is 1: 0.01-10: 0.00001-10.

8. Use of a plant growth regulating composition according to any one of claims 1 to 7 to treat plants.

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