WO2003074715A3 - Methode de production d'acides gras insatures - Google Patents

Methode de production d'acides gras insatures Download PDF

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Publication number
WO2003074715A3
WO2003074715A3 PCT/US2003/005788 US0305788W WO03074715A3 WO 2003074715 A3 WO2003074715 A3 WO 2003074715A3 US 0305788 W US0305788 W US 0305788W WO 03074715 A3 WO03074715 A3 WO 03074715A3
Authority
WO
WIPO (PCT)
Prior art keywords
fatty acids
production
unsaturated fatty
recombinant expression
desaturases
Prior art date
Application number
PCT/US2003/005788
Other languages
English (en)
Other versions
WO2003074715A2 (fr
Inventor
Andreas Renz
Martijn Gipmans
Ivo Feussner
Claire-Lise Rosenfield
Douglas C Knipple
Original Assignee
Basf Plant Science Gmbh
Andreas Renz
Martijn Gipmans
Ivo Feussner
Claire-Lise Rosenfield
Douglas C Knipple
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Gmbh, Andreas Renz, Martijn Gipmans, Ivo Feussner, Claire-Lise Rosenfield, Douglas C Knipple filed Critical Basf Plant Science Gmbh
Priority to CA002477858A priority Critical patent/CA2477858A1/fr
Priority to AU2003219899A priority patent/AU2003219899A1/en
Priority to BR0307938-4A priority patent/BR0307938A/pt
Priority to US10/505,590 priority patent/US7700833B2/en
Priority to JP2003573162A priority patent/JP2006503546A/ja
Priority to EP03716183A priority patent/EP1527182A4/fr
Publication of WO2003074715A2 publication Critical patent/WO2003074715A2/fr
Publication of WO2003074715A3 publication Critical patent/WO2003074715A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fats And Perfumes (AREA)
  • Cosmetics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Cette invention concerne des méthodes de production d'acides gras insaturés, de préférence des acides gras polyinsaturés conjugués, tels que l'acide linoléique conjugué (CLA), par expression recombinante de désaturases provenant d'insectes de l'ordre des lépidoptères. Cette expression intervient, de préférence, dans un organisme choisi parmi des organismes végétaux, des levures, des champignons ou des algues. L'invention concerne également des cassettes d'expression recombinante servant à l'expression recombinante de désaturases provenant d'insectes de l'ordre des lépidoptères. Elle concerne en outre les organismes transgéniques transformés à l'aide de ces cassettes.
PCT/US2003/005788 2002-03-01 2003-02-27 Methode de production d'acides gras insatures WO2003074715A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA002477858A CA2477858A1 (fr) 2002-03-01 2003-02-27 Methode de production d'acides gras insatures
AU2003219899A AU2003219899A1 (en) 2002-03-01 2003-02-27 Process for the production of unsaturated fatty acids
BR0307938-4A BR0307938A (pt) 2002-03-01 2003-02-27 Processo para a produção de triglicerìdeos compreendendo ácidos graxos insaturados, triglicerìdeo, uso do mesmo, polipeptìdeo com atividade e-11 desaturase, sequência de ácido nucleico, cassete de expressão recombinante, vetor de expressão recombinante, e organismo transgênico
US10/505,590 US7700833B2 (en) 2002-03-01 2003-02-27 Process for the production of unsaturated fatty acids
JP2003573162A JP2006503546A (ja) 2002-03-01 2003-02-27 不飽和脂肪酸の製造方法
EP03716183A EP1527182A4 (fr) 2002-03-01 2003-02-27 Methode de production d'acides gras insatures

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10208812A DE10208812A1 (de) 2002-03-01 2002-03-01 Verfahren zur Herstellung von ungesättigten Fettsäuren
DE10208812.8 2002-03-01

Publications (2)

Publication Number Publication Date
WO2003074715A2 WO2003074715A2 (fr) 2003-09-12
WO2003074715A3 true WO2003074715A3 (fr) 2004-04-29

Family

ID=27740519

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/EP2003/002011 WO2003074716A2 (fr) 2002-03-01 2003-02-27 Procedes de production d'acides gras insatures
PCT/US2003/005788 WO2003074715A2 (fr) 2002-03-01 2003-02-27 Methode de production d'acides gras insatures

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/002011 WO2003074716A2 (fr) 2002-03-01 2003-02-27 Procedes de production d'acides gras insatures

Country Status (8)

Country Link
EP (1) EP1527182A4 (fr)
JP (1) JP2006503546A (fr)
CN (1) CN1656226A (fr)
AU (2) AU2003219899A1 (fr)
BR (1) BR0307938A (fr)
CA (1) CA2477858A1 (fr)
DE (1) DE10208812A1 (fr)
WO (2) WO2003074716A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9359430B2 (en) 2006-11-30 2016-06-07 Abbvie Inc. Abeta conformer selective anti-Abeta globulomer monoclonal antibodies
US9540432B2 (en) 2005-11-30 2017-01-10 AbbVie Deutschland GmbH & Co. KG Anti-Aβ globulomer 7C6 antibodies

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3543324B1 (fr) * 2004-02-27 2022-11-30 BASF Plant Science GmbH Procédé de fabrication d'une pluralité d'acides gras insaturés dans les plantes transgéniques
DE102005052551A1 (de) * 2005-11-02 2007-05-16 Rothamsted Res Harpenden Verfahren zur Herstellung von y-Linolensäure und/oder Stearidonsäure in transgenen Brassicaceae und Linaceae
KR20180058863A (ko) 2005-11-30 2018-06-01 애브비 인코포레이티드 아밀로이드 베타 단백질에 대한 모노클로날 항체 및 이의 용도
EA200900568A1 (ru) * 2006-10-20 2010-04-30 Аризона Борд Оф Риджентс Фор Энд Он Бихаф Оф Аризона Стейт Юниверсити Модифицированные цианобактерии
WO2010063451A2 (fr) * 2008-12-01 2010-06-10 Intermed Discovery Gmbh Production d'acides gras oméga-3 par des myxobactéries
JP5168511B2 (ja) * 2009-08-25 2013-03-21 独立行政法人農業生物資源研究所 種子特異的プロモーターおよびその利用
ES2684475T3 (es) 2010-04-15 2018-10-03 Abbvie Inc. Proteínas que se unen a beta amiloide
US11236351B2 (en) 2010-05-17 2022-02-01 Dow Agrosciences Llc Production of DHA and other LC PUFAs in plants
US9062101B2 (en) 2010-08-14 2015-06-23 AbbVie Deutschland GmbH & Co. KG Amyloid-beta binding proteins
TW201307553A (zh) 2011-07-26 2013-02-16 Dow Agrosciences Llc 在植物中生產二十二碳六烯酸（ｄｈａ）及其他長鏈多元不飽和脂肪酸（ｌｃ－ｐｕｆａ）之技術
KR101540746B1 (ko) * 2011-12-28 2015-08-03 대한민국 공액리놀레산(Ｃｏｎｊｕｇａｔｅｄ Ｌｉｎｏｌｅｉｃ Ａｃｉｄ, ＣＬＡ)의 생산방법
WO2016207339A1 (fr) 2015-06-26 2016-12-29 Danmarks Tekniske Universitet Procédé de production de phéromones de papillon de nuit dans de la levure
EP3868890A1 (fr) 2015-11-18 2021-08-25 Provivi, Inc. Micro-organismes destinés à la production de phéromones d'insectes et composés associés
WO2017214133A2 (fr) 2016-06-06 2017-12-14 Provivi, Inc. Production semi-biosynthétique d'alcools gras et d'aldéhydes gras
CN110300799B (zh) 2016-12-16 2024-01-19 丹麦科技大学 在酵母中产生脂肪醇及其衍生物的方法
US11434506B2 (en) 2016-12-16 2022-09-06 Danmarks Tekniske Universitet Production of desaturated fatty alcohols and desaturated fatty alcohol acetates in yeast
BR112019024258A2 (pt) 2017-05-17 2020-08-18 Provivi, Inc. Microorganismos yarrowia lipolytica recombinante e método para produzir um c6-c24 álcool graxo mono- ou poliinsaturado a partir de uma fonte endógena ou exógena de c6-c24 ácidograxo saturado
EP3521439A1 (fr) * 2018-01-31 2019-08-07 Commissariat à l'Energie Atomique et aux Energies Alternatives Procédé de production de triacylglycérides et d'acides gras

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876994A (en) * 1995-11-16 1999-03-02 Cornell Research Foundation, Inc. Pheromone desaturases
US6048838A (en) * 1997-05-05 2000-04-11 Dow Agrosciences Llc Insecticidal protein toxins from xenorhabdus

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0866874B2 (fr) * 1995-11-14 2005-06-22 Loders Croklaan B.V. Procede de preparation de matieres presentant une forte teneur en isomeres d'acide linoleique conjugue
KR100619651B1 (ko) * 1998-03-17 2006-09-05 콘린코 인크. 공액 리놀레산 조성물
AU4564399A (en) * 1998-06-12 1999-12-30 Abbott Laboratories Polyunsaturated fatty acids in plants
AU5785199A (en) * 1998-08-24 2000-03-14 Rutgers, The State University Of New Jersey Synthetic fatty acid desaturase gene for expression in plants
US6706501B1 (en) * 1999-06-30 2004-03-16 Arkion Life Sciences Llc Polynucleotide encoding a propionibacterium linoleate isomerase and uses thereof
CA2378423A1 (fr) * 1999-07-06 2001-01-11 Basf Plant Science Gmbh Plantes exprimant des genes de la .delta.6-desaturase; huiles contenant des acides gras polyinsatures extraites de ces plantes et procede de production d'acides gras insatures
AU2001263473A1 (en) * 2000-05-26 2001-12-11 Washington State University Research Foundation Palmitate desaturase gene

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5876994A (en) * 1995-11-16 1999-03-02 Cornell Research Foundation, Inc. Pheromone desaturases
US6048838A (en) * 1997-05-05 2000-04-11 Dow Agrosciences Llc Insecticidal protein toxins from xenorhabdus

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540432B2 (en) 2005-11-30 2017-01-10 AbbVie Deutschland GmbH & Co. KG Anti-Aβ globulomer 7C6 antibodies
US9359430B2 (en) 2006-11-30 2016-06-07 Abbvie Inc. Abeta conformer selective anti-Abeta globulomer monoclonal antibodies
US9394360B2 (en) 2006-11-30 2016-07-19 Abbvie Inc. Aβ conformer selective anti-Aβ globulomer monoclonal antibodies

Also Published As

Publication number Publication date
WO2003074716A3 (fr) 2003-12-24
AU2003219899A1 (en) 2003-09-16
AU2003208769A1 (en) 2003-09-16
AU2003208769A8 (en) 2003-09-16
WO2003074715A2 (fr) 2003-09-12
CN1656226A (zh) 2005-08-17
DE10208812A1 (de) 2003-09-11
CA2477858A1 (fr) 2003-09-12
EP1527182A4 (fr) 2005-12-21
JP2006503546A (ja) 2006-02-02
WO2003074716A2 (fr) 2003-09-12
EP1527182A2 (fr) 2005-05-04
BR0307938A (pt) 2005-01-11

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