CA2378423A1 - Plantes exprimant des genes de la .delta.6-desaturase; huiles contenant des acides gras polyinsatures extraites de ces plantes et procede de production d'acides gras insatures - Google Patents

Plantes exprimant des genes de la .delta.6-desaturase; huiles contenant des acides gras polyinsatures extraites de ces plantes et procede de production d'acides gras insatures Download PDF

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Publication number
CA2378423A1
CA2378423A1 CA002378423A CA2378423A CA2378423A1 CA 2378423 A1 CA2378423 A1 CA 2378423A1 CA 002378423 A CA002378423 A CA 002378423A CA 2378423 A CA2378423 A CA 2378423A CA 2378423 A1 CA2378423 A1 CA 2378423A1
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Canada
Prior art keywords
leu
ser
val
organism
ala
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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CA002378423A
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English (en)
Inventor
Ernst Heinz
Thomas Girke
Jodi Scheffler
Oswaldo Da Costa E Silva
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF Plant Science GmbH
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Individual
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Filing date
Publication date
Priority claimed from DE10030976A external-priority patent/DE10030976A1/de
Application filed by Individual filed Critical Individual
Publication of CA2378423A1 publication Critical patent/CA2378423A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

L'invention concerne un procédé amélioré de production d'acides gras insaturés et un procédé de production de triglycérides à teneur plus élevée en acides insaturés. L'invention concerne la production d'un organisme transgénique, de préférence, d'une plante transgénique ou d'un micro-organisme transgénique à teneur plus élevée en acides gras, huiles ou lipides avec doubles liaisons .DELTA.6 en raison d'une .DELTA.-6-désaturase exprimée de la mousse. L'invention concerne également des organismes transgéniques qui contiennent un gène de .DELTA.6-désaturase, et l'utilisation des acides gras insaturés et triglycérides ayant une teneur plus élevée en acides gras insaturés obtenus selon le procédé.
CA002378423A 1999-07-06 2000-07-04 Plantes exprimant des genes de la .delta.6-desaturase; huiles contenant des acides gras polyinsatures extraites de ces plantes et procede de production d'acides gras insatures Abandoned CA2378423A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US34753199A 1999-07-06 1999-07-06
US09/347,531 1999-07-06
DE10030976A DE10030976A1 (de) 2000-06-30 2000-06-30 DELTA6-Desaturasegene exprimierende Pflanzen und PUFAS enthaltende Öle aus diesen Pflanzen und ein Verfahren zur Herstellung ungesättigter Fettsäuren
DE10030976.3 2000-06-30
PCT/EP2000/006223 WO2001002591A1 (fr) 1999-07-06 2000-07-04 Plantes exprimant des genes de la δ6-desaturase; huiles contenant des acides gras polyinsatures extraites de ces plantes et procede de production d'acides gras insatures

Publications (1)

Publication Number Publication Date
CA2378423A1 true CA2378423A1 (fr) 2001-01-11

Family

ID=26006195

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002378423A Abandoned CA2378423A1 (fr) 1999-07-06 2000-07-04 Plantes exprimant des genes de la .delta.6-desaturase; huiles contenant des acides gras polyinsatures extraites de ces plantes et procede de production d'acides gras insatures

Country Status (4)

Country Link
EP (1) EP1190080A1 (fr)
AU (1) AU776417B2 (fr)
CA (1) CA2378423A1 (fr)
WO (1) WO2001002591A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7700833B2 (en) 2002-03-01 2010-04-20 Cornell University Process for the production of unsaturated fatty acids
US7893320B2 (en) 2002-04-29 2011-02-22 Basf Plant Science Gmbh Method for producing multiple unsaturated fatty acids in plants
US8629195B2 (en) 2006-04-08 2014-01-14 Bayer Materialscience Ag Production of polyurethane foams
US9458477B2 (en) 2010-10-21 2016-10-04 Basf Plant Science Company Gmbh Fatty acid desaturases, elongases, elongation components and uses thereof

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10102338A1 (de) * 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Expression von Biosynthesegenen in pflanzlichen Samen unter Verwendung von neuen multiplen Expressionskonstrukten
GB2385852A (en) * 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
DE10208812A1 (de) * 2002-03-01 2003-09-11 Basf Plant Science Gmbh Verfahren zur Herstellung von ungesättigten Fettsäuren
CA2556336C (fr) * 2004-02-17 2015-09-08 The University Of York Enzymes desaturases
ES2529572T3 (es) 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
CA2563875C (fr) 2004-04-22 2015-06-30 Commonwealth Scientific And Industrial Research Organisation Synthese d'acides gras polyinsatures a chaine longue par des cellules de recombinaison
PL1766023T3 (pl) 2004-06-04 2011-04-29 Fluxome Sciences As Metabolicznie zmodyfikowane komórki do wytwarzania wielonienasyconych kwasów tłuszczowych
CN101578363A (zh) 2006-08-29 2009-11-11 联邦科学技术研究组织 脂肪酸的合成
EP2318533A2 (fr) 2008-07-01 2011-05-11 BASF Plant Science GmbH Promoteurs de brassica napus pour l'expression de gènes spécifique de graines
US8809559B2 (en) 2008-11-18 2014-08-19 Commonwelath Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
CA2760326A1 (fr) 2009-05-13 2010-11-18 Basf Plant Science Company Gmbh Acyltransferases et leurs utilisations dans la production d'acide gras
DE112010002353T5 (de) 2009-06-08 2012-08-09 Basf Plant Science Company Gmbh Neue fettsäure-elongations-komponenten und anwenduingen davon
DE112010002967T5 (de) 2009-07-17 2012-10-11 Basf Plant Science Company Gmbh Neue Fettsäuredesaturasen und -elongasen und Anwendungen davon
CN102597245A (zh) 2009-08-31 2012-07-18 巴斯夫植物科学有限公司 用于在植物中增强种子特异性基因表达而促进增强的多不饱和脂肪酸合成的调节性核酸分子
US9347049B2 (en) 2009-11-24 2016-05-24 Basf Plant Science Company Gmbh Fatty acid elongase and uses thereof
EP2504427B1 (fr) 2009-11-24 2018-06-27 BASF Plant Science Company GmbH Nouvelle désaturase d'acides gras et ses utilisations
NO2585603T3 (fr) 2010-06-25 2018-05-19
PL2861059T3 (pl) 2012-06-15 2017-10-31 Commw Scient Ind Res Org Wytwarzanie długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach roślinnych
WO2014020533A2 (fr) 2012-08-03 2014-02-06 Basf Plant Science Company Gmbh Nouvelles enzymes, composants enzymatiques et leurs utilisations
KR102535223B1 (ko) 2013-12-18 2023-05-30 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 장쇄 다중불포화 지방산을 포함하는 지질
CN105219789B (zh) 2014-06-27 2023-04-07 联邦科学技术研究组织 包含二十二碳五烯酸的提取的植物脂质

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5614393A (en) * 1991-10-10 1997-03-25 Rhone-Poulenc Agrochimie Production of γ-linolenic acid by a Δ6-desaturase
NZ337459A (en) * 1997-04-11 2000-07-28 Abbott Lab Nucleic acid construct in plants and dietary supplement
GB9724783D0 (en) * 1997-11-24 1998-01-21 Inst Arable Crops Research Novel polypeptides

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7700833B2 (en) 2002-03-01 2010-04-20 Cornell University Process for the production of unsaturated fatty acids
US7893320B2 (en) 2002-04-29 2011-02-22 Basf Plant Science Gmbh Method for producing multiple unsaturated fatty acids in plants
US8629195B2 (en) 2006-04-08 2014-01-14 Bayer Materialscience Ag Production of polyurethane foams
US9458477B2 (en) 2010-10-21 2016-10-04 Basf Plant Science Company Gmbh Fatty acid desaturases, elongases, elongation components and uses thereof

Also Published As

Publication number Publication date
EP1190080A1 (fr) 2002-03-27
WO2001002591A1 (fr) 2001-01-11
AU6560700A (en) 2001-01-22
AU776417B2 (en) 2004-09-09

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