WO2003068954A8 - Mikroorganismus zur gentherapeutischen behandlung proliferativer erkrankungen - Google Patents

Mikroorganismus zur gentherapeutischen behandlung proliferativer erkrankungen

Info

Publication number
WO2003068954A8
WO2003068954A8 PCT/DE2003/000470 DE0300470W WO03068954A8 WO 2003068954 A8 WO2003068954 A8 WO 2003068954A8 DE 0300470 W DE0300470 W DE 0300470W WO 03068954 A8 WO03068954 A8 WO 03068954A8
Authority
WO
WIPO (PCT)
Prior art keywords
microorganism
nucleotide sequence
specific
optionally
expression
Prior art date
Application number
PCT/DE2003/000470
Other languages
English (en)
French (fr)
Other versions
WO2003068954A3 (de
WO2003068954A2 (de
Inventor
Werner Goebel
R Ulf Rapp
Hans-Harald Sedlacek
Joachim Fensterle
Ivaylo Gentschev
Original Assignee
Medinnova Ges Med Innovationen
Werner Goebel
R Ulf Rapp
Hans-Harald Sedlacek
Joachim Fensterle
Ivaylo Gentschev
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP03704314A priority Critical patent/EP1474519A2/de
Priority to BRPI0307722A priority patent/BRPI0307722A2/pt
Priority to DE2003190506 priority patent/DE10390506D2/de
Priority to US10/504,944 priority patent/US20050244374A1/en
Priority to AU2003206663A priority patent/AU2003206663B2/en
Priority to KR10-2004-7012866A priority patent/KR20040103941A/ko
Priority to YU72404A priority patent/RS72404A/sr
Priority to CA002513198A priority patent/CA2513198A1/en
Application filed by Medinnova Ges Med Innovationen, Werner Goebel, R Ulf Rapp, Hans-Harald Sedlacek, Joachim Fensterle, Ivaylo Gentschev filed Critical Medinnova Ges Med Innovationen
Priority to JP2003568069A priority patent/JP2005517405A/ja
Priority to MXPA04007934A priority patent/MXPA04007934A/es
Priority to NZ535310A priority patent/NZ535310A/en
Priority to IL16355303A priority patent/IL163553A0/xx
Publication of WO2003068954A2 publication Critical patent/WO2003068954A2/de
Publication of WO2003068954A3 publication Critical patent/WO2003068954A3/de
Priority to NO20043800A priority patent/NO20043800L/no
Priority to HRP20040832 priority patent/HRP20040832A2/hr
Publication of WO2003068954A8 publication Critical patent/WO2003068954A8/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01031—Beta-glucuronidase (3.2.1.31)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70575—NGF/TNF-superfamily, e.g. CD70, CD95L, CD153, CD154
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/16—Exonucleases active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters (3.16)
    • C12Y301/16001—Spleen exonuclease (3.1.16.1), i.e. 5->3 exoribonuclease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Epidemiology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Rheumatology (AREA)
  • Oncology (AREA)
  • Pain & Pain Management (AREA)
  • Transplantation (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)

Abstract

Die Erfindung lehrt einen ummantelten Mikroorganismus, in dessen Genom folgende Komponenten eingefügt und exprimierbar sind: I) eine Nukleotidsequenz, kodierend für ein di­rekt oder indirekt, antiproliferativ oder zytotoxisch wirkendes Expressionsprodukt oder für mehrere verschiedene solcher Expressionsprodukte, II) eine Nukleotidsequenz, welche für ein Blutplasmaprotein unter der Kontrolle einer im Mikroorganismus aktivierbaren Aktivierungssequenz kod­iert oder konstitutiv aktiv ist, III) optional, eine Nuk­leotidsequenz, welche für einen zellspezifischen Liganden unter der Kontrolle einer im Mikroorganismus aktivierbaren Aktivierungssequenz kodiert oder konstitutiv aktiv ist, IV) eine Nukleotidsequenz für ein Transportsystem, welches die Expression der Expressionsprodukte der Komponenten (I) und (II) sowie optional (III) auf der Außenfläche des Mikro­organismus oder die Sekretion der Expressionsprodukte der Komponente (I) und Expression der Komponente (II) sowie op­tional (III) bewirkt und vorzugsweise konstitutiv aktiv ist, V) optional eine Nukleotidsequenz für ein Protein zur Lyse des Mikrooganismus im Cytosol von Säugerzellen und zur intrazellulären Freisetzung von Plasmiden mit zumind­est einer oder mehreren der Komponenten (I) und (VI) enthalten in dem lysierten Mikroorganismus, und VI) eine in dem Mikroorganismus aktivierbare, und/oder eine gewebezellspezifische, tumorzellspezifische, funktionsspezi­fische oder nicht zellspezifische Aktivierungssequenz zur Expression von Komponente I), wobei jede der Komponenten (I) bis (VI) einfach oder mehrfach, jeweils gleich oder ver­schieden, eingerichtet sein kann.
PCT/DE2003/000470 2002-02-14 2003-02-13 Mikroorganismus zur gentherapeutischen behandlung proliferativer erkrankungen WO2003068954A2 (de)

Priority Applications (14)

Application Number Priority Date Filing Date Title
JP2003568069A JP2005517405A (ja) 2002-02-14 2003-02-13 増殖性疾患の遺伝子療法的治療のための微生物
BRPI0307722A BRPI0307722A2 (pt) 2002-02-14 2003-02-13 microorganismo envolvido
MXPA04007934A MXPA04007934A (es) 2002-02-14 2003-02-13 Microorganismo envuelto.
AU2003206663A AU2003206663B2 (en) 2002-02-14 2003-02-13 Microorganism for genetic therapeutic treatment of proliferative diseases
KR10-2004-7012866A KR20040103941A (ko) 2002-02-14 2003-02-13 증식성 질병의 유전자 치료를 위한 미생물
YU72404A RS72404A (en) 2002-02-14 2003-02-13 Microorganism for genetic therapeutic treatment of proliferative diseases
CA002513198A CA2513198A1 (en) 2002-02-14 2003-02-13 Microorganism for genetic therapeutic treatment of proliferative diseases
EP03704314A EP1474519A2 (de) 2002-02-14 2003-02-13 Mikroorganismus zur gentherapeutischen behandlung proliferativer erkrankungen
DE2003190506 DE10390506D2 (de) 2002-02-14 2003-02-13 Ummantelter Mikroorganismus
US10/504,944 US20050244374A1 (en) 2002-02-14 2003-02-13 Enveloped miroorganism
NZ535310A NZ535310A (en) 2002-02-14 2003-02-13 Microorganism for genetic therapeutic treatment of proliferative diseases
IL16355303A IL163553A0 (en) 2002-02-14 2003-08-13 Microorganism for genetic therapeutic treatment ofproliferative diseases
NO20043800A NO20043800L (no) 2002-02-14 2004-09-10 Mikroorganisme for genetisk terapetuisk behandling av proliferativ sykdom
HRP20040832 HRP20040832A2 (en) 2002-02-14 2004-09-14 Microorganism for genetic therapeutic treatment of proliferative diseases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10206325.7 2002-02-14
DE10206325A DE10206325A1 (de) 2002-02-14 2002-02-14 Ummantelter Mikroorganismus

Publications (3)

Publication Number Publication Date
WO2003068954A2 WO2003068954A2 (de) 2003-08-21
WO2003068954A3 WO2003068954A3 (de) 2003-10-16
WO2003068954A8 true WO2003068954A8 (de) 2005-10-13

Family

ID=27674670

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2003/000470 WO2003068954A2 (de) 2002-02-14 2003-02-13 Mikroorganismus zur gentherapeutischen behandlung proliferativer erkrankungen

Country Status (19)

Country Link
US (1) US20050244374A1 (de)
EP (1) EP1474519A2 (de)
JP (1) JP2005517405A (de)
KR (1) KR20040103941A (de)
CN (1) CN1646693A (de)
AU (1) AU2003206663B2 (de)
BR (1) BRPI0307722A2 (de)
CA (1) CA2513198A1 (de)
DE (2) DE10206325A1 (de)
HR (1) HRP20040832A2 (de)
IL (1) IL163553A0 (de)
MX (1) MXPA04007934A (de)
NO (1) NO20043800L (de)
NZ (1) NZ535310A (de)
PL (1) PL372901A1 (de)
RS (1) RS72404A (de)
RU (1) RU2004127459A (de)
WO (1) WO2003068954A2 (de)
ZA (1) ZA200407358B (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008504822A (ja) * 2004-06-29 2008-02-21 アンチキャンサー インコーポレーテッド 癌選択的栄養要求株
CN112423785A (zh) 2018-07-19 2021-02-26 瑞泽恩制药公司 双特异性抗BCMAx抗CD3抗体及其用途
US11519007B2 (en) 2019-02-22 2022-12-06 Arizona Board Of Regents On Behalf Of Arizona State University Tumor-navigating, self-eradicating, trail-armed salmonella for precision cancer therapy

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19720761A1 (de) * 1997-05-07 1998-11-12 Schering Ag Verfahren zur Synthese und Sekretion von zur Kontrazeption geeigneter Proteine oder Proteinfragmente durch attenuierte Salmonellen oder einen anderen Gram-negativen attenuierten Impfstamm zur Erzeugung einer oralen Vakzinierung

Also Published As

Publication number Publication date
AU2003206663B2 (en) 2007-11-01
KR20040103941A (ko) 2004-12-09
AU2003206663A1 (en) 2003-09-04
CA2513198A1 (en) 2003-08-21
DE10206325A1 (de) 2003-09-04
BRPI0307722A2 (pt) 2017-07-04
RS72404A (en) 2006-12-15
JP2005517405A (ja) 2005-06-16
WO2003068954A3 (de) 2003-10-16
US20050244374A1 (en) 2005-11-03
EP1474519A2 (de) 2004-11-10
MXPA04007934A (es) 2005-11-23
HRP20040832A2 (en) 2004-12-31
NZ535310A (en) 2008-04-30
RU2004127459A (ru) 2005-05-10
PL372901A1 (en) 2005-08-08
DE10390506D2 (de) 2005-01-13
AU2003206663B9 (en) 2003-09-04
WO2003068954A2 (de) 2003-08-21
CN1646693A (zh) 2005-07-27
ZA200407358B (en) 2005-11-18
IL163553A0 (en) 2005-12-18
NO20043800L (no) 2004-11-08

Similar Documents

Publication Publication Date Title
Margottin et al. Participation of the TATA factor in transcription of the yeast U6 gene by RNA polymerase C
Berrocal-Tito et al. Trichoderma atroviride PHR1, a fungal photolyase responsible for DNA repair, autoregulates its own photoinduction
Hübscher et al. Replication of the lagging strand: a concert of at least 23 polypeptides
ATE323169T1 (de) Plasmid zur verabreichung von nukleine säuren und verwendungsverfahren
AU3461097A (en) Novel VEGF-like factor
MX9705039A (es) Genes y proteinas de muerte celular programada.
WO2002042445A3 (en) Differentiated cells suitable for human therapy
EP0919622A3 (de) Oberflächenlokalisierte Expression von Enzymen in gengesteuerter Prodrugtherapie
WO2003023000A3 (en) Linear dna fragments for gene expression
WO2001002019A3 (en) Control of gene expression
WO2003045427A3 (en) Pharmaceutical composition for inducing an immune response in a human or animal
MX9708854A (es) Terapia de genes para regulacion de celulas efectoras.
WO2000056866A3 (en) Akt-3 nucleic acids, polypeptides, and uses thereof
WO2003068954A3 (de) Mikroorganismus zur gentherapeutischen behandlung proliferativer erkrankungen
Meineke et al. A fungal anticodon nuclease ribotoxin exploits a secondary cleavage site to evade tRNA repair
SG93780A1 (en) Gene manipulation and expression using genomic elements
AU2180501A (en) Novel DNA polymerase inhibitors, mikanolide and dihydromikanolide
WO2007089950A3 (en) Preparations of growth hormone
GB0017761D0 (en) Nucleic acid delivery
EP1188835A4 (de) Vektor für die expression von zwei fremdgenen
WO2004024867A3 (en) Vigilant vector system
WO2001025398A3 (en) Process for inducing functional tolerance to gene transfer products
EP0763205A4 (de) Nukleinsäuresequenzen die lungenzellenspezifishe genexpression kontrollieren
WO2004096826A3 (en) Method for selective inhibition of human n-myc gene in n-myc expressing tumors through antisense and antigen peptido-nucleic acids (pna)
AU2944100A (en) Gene therapeutics

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: P-724/04

Country of ref document: YU

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 1020047012866

Country of ref document: KR

WWE Wipo information: entry into national phase

Ref document number: 163553

Country of ref document: IL

WWE Wipo information: entry into national phase

Ref document number: PA/a/2004/007934

Country of ref document: MX

Ref document number: 2003568069

Country of ref document: JP

Ref document number: 372901

Country of ref document: PL

WWE Wipo information: entry into national phase

Ref document number: 2003704314

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 01349/KOLNP/2004

Country of ref document: IN

Ref document number: 1349/KOLNP/2004

Country of ref document: IN

WWE Wipo information: entry into national phase

Ref document number: 2004/07358

Country of ref document: ZA

Ref document number: P20040832A

Country of ref document: HR

Ref document number: 535310

Country of ref document: NZ

Ref document number: 2004127459

Country of ref document: RU

Ref document number: 2003206663

Country of ref document: AU

Ref document number: 200407358

Country of ref document: ZA

WWE Wipo information: entry into national phase

Ref document number: 20038082446

Country of ref document: CN

WWP Wipo information: published in national office

Ref document number: 2003704314

Country of ref document: EP

REF Corresponds to

Ref document number: 10390506

Country of ref document: DE

Date of ref document: 20050113

Kind code of ref document: P

WWE Wipo information: entry into national phase

Ref document number: 10390506

Country of ref document: DE

WWE Wipo information: entry into national phase

Ref document number: 10504944

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2513198

Country of ref document: CA

CFP Corrected version of a pamphlet front page
CR1 Correction of entry in section i

Free format text: IN PCT GAZETTE 34/2003 UNDER (81) DELETE "DE"

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

ENP Entry into the national phase

Ref document number: 2003206663

Country of ref document: AU

Date of ref document: 20030213

Kind code of ref document: B

ENP Entry into the national phase

Ref document number: PI0307722

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20040816