WO2003065031A1 - Procede et dispositif pour analyser de l'acide amine, un peptide, une proteine, une saccharide ou un lipide - Google Patents

Procede et dispositif pour analyser de l'acide amine, un peptide, une proteine, une saccharide ou un lipide Download PDF

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Publication number
WO2003065031A1
WO2003065031A1 PCT/JP2003/000974 JP0300974W WO03065031A1 WO 2003065031 A1 WO2003065031 A1 WO 2003065031A1 JP 0300974 W JP0300974 W JP 0300974W WO 03065031 A1 WO03065031 A1 WO 03065031A1
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WO
WIPO (PCT)
Prior art keywords
phosphorylated
amino acid
peptide
protein
column
Prior art date
Application number
PCT/JP2003/000974
Other languages
English (en)
French (fr)
Inventor
Ikuma Kuroda
Kouji Takahashi
Hiroshi Nakamura
Original Assignee
Gl Sciences Incorporated
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gl Sciences Incorporated filed Critical Gl Sciences Incorporated
Priority to US10/503,349 priority Critical patent/US20050170525A1/en
Priority to EP20030703116 priority patent/EP1477800A4/en
Priority to JP2003564576A priority patent/JP4187106B2/ja
Publication of WO2003065031A1 publication Critical patent/WO2003065031A1/ja

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/0203—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of metals not provided for in B01J20/04
    • B01J20/0211—Compounds of Ti, Zr, Hf
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/06—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising oxides or hydroxides of metals not provided for in group B01J20/04
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/08—Preparation using an enricher
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/08—Preparation using an enricher
    • G01N2030/085—Preparation using an enricher using absorbing precolumn
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8818—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8836—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving saccharides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/466—Flow patterns using more than one column with separation columns in parallel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/468—Flow patterns using more than one column involving switching between different column configurations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Inorganic Chemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Peptides Or Proteins (AREA)
PCT/JP2003/000974 2002-01-31 2003-01-31 Procede et dispositif pour analyser de l'acide amine, un peptide, une proteine, une saccharide ou un lipide WO2003065031A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/503,349 US20050170525A1 (en) 2002-01-31 2003-01-31 Method and apparatus for analyzing amino acid, peptide, protein, saccharide or lipid
EP20030703116 EP1477800A4 (en) 2002-01-31 2003-01-31 METHOD AND APPARATUS FOR AMINO ACID, PEPTIDE, PROTEIN, SACCHARIDE OR LIPID ANALYSIS
JP2003564576A JP4187106B2 (ja) 2002-01-31 2003-01-31 アミノ酸、ペプチド、タンパク質、糖又は脂質分析方法及び装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002-24498 2002-01-31
JP2002024498 2002-01-31

Publications (1)

Publication Number Publication Date
WO2003065031A1 true WO2003065031A1 (fr) 2003-08-07

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PCT/JP2003/000974 WO2003065031A1 (fr) 2002-01-31 2003-01-31 Procede et dispositif pour analyser de l'acide amine, un peptide, une proteine, une saccharide ou un lipide

Country Status (4)

Country Link
US (1) US20050170525A1 (ja)
EP (1) EP1477800A4 (ja)
JP (1) JP4187106B2 (ja)
WO (1) WO2003065031A1 (ja)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004271273A (ja) * 2003-03-06 2004-09-30 Japan Science & Technology Agency 高等植物の糖リン酸の網羅的分析法
WO2006004214A1 (ja) * 2004-07-02 2006-01-12 Eisai R & D Management Co., Ltd. リン酸化タンパク質のプロテオーム解析方法
WO2008020603A1 (fr) * 2006-08-17 2008-02-21 Keio University Procédé de séparation d'un peptide phosphorylé ou d'une protéine phosphorylée
JP2008506929A (ja) * 2004-07-06 2008-03-06 パーキンエルマー・エルエイエス・インコーポレイテッド 水和金属酸化物を用いるリン酸化分子の検出と単離のための方法と組成物
JP2008058207A (ja) * 2006-09-01 2008-03-13 Sumika Chemical Analysis Service Ltd カートリッジ及び該カートリッジを具備するカドミウム分析用前処理装置
JP2008156571A (ja) * 2006-12-26 2008-07-10 Aichi Prefecture リン脂質及び糖脂質の吸着分離方法
JP2008249447A (ja) * 2007-03-30 2008-10-16 Hitachi High-Technologies Corp アミノ酸分析法およびアミノ酸分析システム
JP2008309547A (ja) * 2007-06-13 2008-12-25 Hitachi High-Technologies Corp 修飾ペプチドの分析方法および装置
JP2009121857A (ja) * 2007-11-13 2009-06-04 Shimadzu Corp リン酸化ペプチド測定方法
JP2014092523A (ja) * 2012-11-06 2014-05-19 Shimadzu Corp 液体クロマトグラフィー−ｍａｌｄｉ質量分析法
JP2017036369A (ja) * 2015-08-07 2017-02-16 株式会社イノアック技術研究所 可溶性のリン酸化キチンまたは可溶性のリン酸化コラーゲンを製造する方法、リン酸化キチンまたはリン酸化コラーゲンとチタンとの複合体を製造する方法、ポリウレタン発泡体を製造する方法、複合体、リン酸化コラーゲンを製造する方法およびリン酸化コラーゲンとチタンとの複合体を製造する方法
CN111624279A (zh) * 2020-05-22 2020-09-04 海南医学院 一种生物样品中喹诺酮类抗生素的前处理方法及其定量检测方法

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DE10322701B4 (de) * 2003-05-20 2006-12-28 Humboldt-Universität Zu Berlin Probenträger unter Verwendung eines porösen, Metalloxidpartikel umfassenden Films, Verfahren zur Herstellung eines Probenträgers, Verwendung des Probenträgers sowie Verfahren zum selektiven Nachweis von phosphorylierten/sulfatierten Biopolymeren, insbesondere Peptiden/Proteinen
WO2007124750A2 (en) * 2006-04-27 2007-11-08 Syddansk Universitet Methods for isolation and analysis of sialylated and phosphorylated peptides
US8382987B2 (en) * 2006-09-27 2013-02-26 Alessandra Luchini Method for harvesting nanoparticles and sequestering biomarkers
US8632883B2 (en) 2008-02-15 2014-01-21 Ge Healthcare Bio-Sciences Ab Separation media for biomolecules comprising porous hybrid particles containing inorganic metal oxide nanoparticles
US20110070663A1 (en) * 2008-05-26 2011-03-24 Merck Patent Gesellschaft Mit Beschrankter Haftung Method for the enrichment of phosphorylated and/or glycosylated analytes
US9182342B2 (en) * 2011-09-23 2015-11-10 Motion Controls, Llc Apparatus, system and method for using an LED to identify a presence of a material in a gas and/or a fluid and/or determine properties of the material
CN104391053B (zh) * 2014-10-30 2016-02-03 广东新峰药业股份有限公司 一种同时测定地龙注射剂中五种氨基酸含量的pr-hplc测定方法
JP7388206B2 (ja) * 2020-01-22 2023-11-29 株式会社島津製作所 液体クロマトグラフおよび分析方法
WO2023281422A1 (en) * 2021-07-06 2023-01-12 Waters Technologies Corporation Use of neutral ph mobile phases in reversed phase chromatography of acidic peptides
CN114019034A (zh) * 2021-09-14 2022-02-08 上海中科新生命生物科技有限公司 一种游离氨基酸快速非衍生的液相色谱串联质谱检测方法

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DK182289D0 (da) * 1989-04-14 1989-04-14 Francesco Blasi Modifikation af proteiner

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AKIRA SANO, HIROSHI NAKAMURA, CHROMATOGRAPHY, vol. 17, no. 4, 1996, pages 354 - 355, XP002967024 *
HIROSHI NAKAMURA: "Biryo genso no kagakuteki sonkeitai shikibetsu no tameno sentakuteki na bunri.bunsekiho kaihatsu ni kansuru kenkyu", HEISEI 7-8 NENDO KAGAKU KENKYUHI HOJOKIN (KIBAN KENKYU (A) 1), 1997, pages 59 - 64, XP002967026 *
See also references of EP1477800A4 *
YOSHIHIKO IKEGUTHI, HIROSHI NAKAMURA: "Determination of Organic Phosphates by Column-Switching High Performance Anion- Exhange Chromatography Using On-Line Preconcentration on Titania.", ANALYTICAL SCIENCES, vol. 13, no. 3, June 1997 (1997-06-01), pages 479 - 483, XP002967025 *

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004271273A (ja) * 2003-03-06 2004-09-30 Japan Science & Technology Agency 高等植物の糖リン酸の網羅的分析法
WO2006004214A1 (ja) * 2004-07-02 2006-01-12 Eisai R & D Management Co., Ltd. リン酸化タンパク質のプロテオーム解析方法
JPWO2006004214A1 (ja) * 2004-07-02 2008-04-24 エーザイ・アール・アンド・ディー・マネジメント株式会社 リン酸化タンパク質のプロテオーム解析方法
JP2008506929A (ja) * 2004-07-06 2008-03-06 パーキンエルマー・エルエイエス・インコーポレイテッド 水和金属酸化物を用いるリン酸化分子の検出と単離のための方法と組成物
US8945932B2 (en) 2004-07-06 2015-02-03 Perkinelmer Health Sciences, Inc. Methods and compositions for detecting and isolating phosphorylated molecules using hydrated metal oxides
JP5273658B2 (ja) * 2006-08-17 2013-08-28 学校法人慶應義塾 リン酸化ペプチド又はリン酸化タンパク質の分離方法
WO2008020603A1 (fr) * 2006-08-17 2008-02-21 Keio University Procédé de séparation d'un peptide phosphorylé ou d'une protéine phosphorylée
JP2008058207A (ja) * 2006-09-01 2008-03-13 Sumika Chemical Analysis Service Ltd カートリッジ及び該カートリッジを具備するカドミウム分析用前処理装置
JP2008156571A (ja) * 2006-12-26 2008-07-10 Aichi Prefecture リン脂質及び糖脂質の吸着分離方法
JP2008249447A (ja) * 2007-03-30 2008-10-16 Hitachi High-Technologies Corp アミノ酸分析法およびアミノ酸分析システム
JP2008309547A (ja) * 2007-06-13 2008-12-25 Hitachi High-Technologies Corp 修飾ペプチドの分析方法および装置
JP2009121857A (ja) * 2007-11-13 2009-06-04 Shimadzu Corp リン酸化ペプチド測定方法
JP2014092523A (ja) * 2012-11-06 2014-05-19 Shimadzu Corp 液体クロマトグラフィー−ｍａｌｄｉ質量分析法
JP2017036369A (ja) * 2015-08-07 2017-02-16 株式会社イノアック技術研究所 可溶性のリン酸化キチンまたは可溶性のリン酸化コラーゲンを製造する方法、リン酸化キチンまたはリン酸化コラーゲンとチタンとの複合体を製造する方法、ポリウレタン発泡体を製造する方法、複合体、リン酸化コラーゲンを製造する方法およびリン酸化コラーゲンとチタンとの複合体を製造する方法
CN111624279A (zh) * 2020-05-22 2020-09-04 海南医学院 一种生物样品中喹诺酮类抗生素的前处理方法及其定量检测方法
CN111624279B (zh) * 2020-05-22 2022-06-10 海南医学院 一种生物样品中喹诺酮类抗生素的前处理方法及其定量检测方法

Also Published As

Publication number Publication date
US20050170525A1 (en) 2005-08-04
EP1477800A1 (en) 2004-11-17
JP4187106B2 (ja) 2008-11-26
JPWO2003065031A1 (ja) 2005-05-26
EP1477800A4 (en) 2005-08-17
EP1477800A8 (en) 2005-02-23

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