WO2003024213A1 - Boitier de protection et de transport de greffons d'epiderme ou de peau cultives in vitro a partir d'un greffon de peau preleve sur un donneur - Google Patents

Boitier de protection et de transport de greffons d'epiderme ou de peau cultives in vitro a partir d'un greffon de peau preleve sur un donneur Download PDF

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Publication number
WO2003024213A1
WO2003024213A1 PCT/IT2001/000482 IT0100482W WO03024213A1 WO 2003024213 A1 WO2003024213 A1 WO 2003024213A1 IT 0100482 W IT0100482 W IT 0100482W WO 03024213 A1 WO03024213 A1 WO 03024213A1
Authority
WO
WIPO (PCT)
Prior art keywords
lid
tray
case
epidermis
case according
Prior art date
Application number
PCT/IT2001/000482
Other languages
English (en)
Inventor
Michele De Luca
Graziella Pellegrini
Original Assignee
Provincia Italiana Della Congregazione Dei Figli Dell'immacolata Concezione - Istituto Dermopatico Dell'immacolata
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Provincia Italiana Della Congregazione Dei Figli Dell'immacolata Concezione - Istituto Dermopatico Dell'immacolata filed Critical Provincia Italiana Della Congregazione Dei Figli Dell'immacolata Concezione - Istituto Dermopatico Dell'immacolata
Priority to PCT/IT2001/000482 priority Critical patent/WO2003024213A1/fr
Publication of WO2003024213A1 publication Critical patent/WO2003024213A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0236—Mechanical aspects
    • A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
    • A01N1/0273—Transport containers

Definitions

  • the present finding relates to the skin engineering and epidermis grafts and particularly a case able to receive and to protect epidermis or skin grafts cultivated in vitro both during their storing in the refrigerator and the transportation from the production and/or storing site to the repair site where they will be grafted to the donor patient .
  • Another problem is to find epidermis or skin grafts that do not causd any rejection.
  • the present techniques of skin engineering have overcome such problems by providing in vitro epidermis or skin cultivation methods starting from an even small epidermis or skin graft of the patient to be treated.
  • the present finding seeks to overcome said problems by providing a case which can be easily stacked and capable of protecting and sealing the single epidermis or skin grafts placed in the preservation solution even during any transfer to an incubator, thus avoiding their curling and wrinkling both during the transportation and the storage as well as the escape of gases contained in the preservation solution and the inlet of the atmosphere gases.
  • Figure 1 is a top plan view of the upper portion or lid of the case according to the finding
  • Figure 2 is a cross section of the lid along the plane A-A of Figure 1;
  • Figure 3 is a top plan view of the lower portion or tray of the case according to the finding;
  • Figure 4 is a cross section of the tray along the plane B-B of Figure 3 ;
  • Figure 5 is a cross section similar to Figures 2 and 4 of the superimposed tray and lid in the transportation position;
  • Figure 6 is an enlarged detail of the section of Figure 5 ;
  • Figure 7 is a top plan view of several cases stacked on one another according to the finding
  • Figure 8 is a cross section of the stacked cases of Figure 7 along the plane C-C.
  • the case consists of a lid 1 and a tray 2 able to be superimposed and fitted on each other so as to define a closed, essentially flat space V (dashed in Figure 5) able to contain the epidermis or skin graft cultivated in vitro and immersed in a culture liquid under stretched conditions.
  • said space is small enough to avoid the wrinkling of the skin graft contained therein, even if the case is inclined.
  • the lid has an essentially flat central portion which is lowered with respect to its peripheral edge and is provided with a continuos peripheral little channel which projects from the underlying surface so as to form a corresponding peripheral lower rib 3 projecting from the flat surface of the lowered portion of the lid.
  • Tray 2 shown in Figures 3 and 4 has a flat bottom surrounded and delimited by a continuous peripheral rib 4 which is raised with respect to the bottom and outside which there is provided a small channel 5 running parallel to said rib 4 and able to fit such lower rib 3 of lid 1.
  • the bottom of tray 2 is deeper than the peripheral small channel 5.
  • space V able to receive the cultivated epidermis or skin graft and the culture liquid necessary to its preservation is defined by the empty space delimited at the lower side by the central portion of tray 2 and at the upper side by the flat bottom of lid 1.
  • Such lid 1 and such tray 2 are superimposed by fitting rib 3 into small channel 5, as described above.
  • the case is made of a chemically and biologically inert material which is impermeable to liquids and gases.
  • the liquid and gas sealing is advantageously ensured by that the peripheral portions of lid 1 and tray 2 are shaped such as to provide perfectly conjugated surfaces ensuring the sealing by fitting rib 3 into small channel 5, as described above.
  • the liquid and gas impermeable material forming the case is preferably transparent PET or other material which is chemically and biologically inert both at the temperature of use, particularly between -80 °C and +35°C and at the heat sealing temperature.

Abstract

La présente invention concerne un boîtier de protection et de transport de greffons d'épiderme ou de peau, qui comprend un couvercle (1) et un plateau (2) qu'on peut superposer et ajuster l'un sur l'autre de façon à définir un espace (V) clos, sensiblement plat, capable de contenir le greffon tendu d'épiderme ou de peau immergé dans un liquide de conservation. Ce couvercle (1) et ce plateau (2) sont pourvus d'un rebord (F) périphérique qu'on peut thermosouder. L'espace (V) est suffisamment petit pour éviter le plissement du greffon de peau contenu, même si on incline le boîtier. Ce boîtier est fabriqué dans un matériau transparent imperméable aux liquides et aux gaz, tel qu'un PET ou un autre matériau chimiquement et biologiquement inerte, tant à la température d'utilisation qu'à celle du thermosoudage.
PCT/IT2001/000482 2001-09-17 2001-09-17 Boitier de protection et de transport de greffons d'epiderme ou de peau cultives in vitro a partir d'un greffon de peau preleve sur un donneur WO2003024213A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/IT2001/000482 WO2003024213A1 (fr) 2001-09-17 2001-09-17 Boitier de protection et de transport de greffons d'epiderme ou de peau cultives in vitro a partir d'un greffon de peau preleve sur un donneur

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IT2001/000482 WO2003024213A1 (fr) 2001-09-17 2001-09-17 Boitier de protection et de transport de greffons d'epiderme ou de peau cultives in vitro a partir d'un greffon de peau preleve sur un donneur

Publications (1)

Publication Number Publication Date
WO2003024213A1 true WO2003024213A1 (fr) 2003-03-27

Family

ID=11133727

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2001/000482 WO2003024213A1 (fr) 2001-09-17 2001-09-17 Boitier de protection et de transport de greffons d'epiderme ou de peau cultives in vitro a partir d'un greffon de peau preleve sur un donneur

Country Status (1)

Country Link
WO (1) WO2003024213A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009155430A2 (fr) * 2008-06-18 2009-12-23 The Cleveland Clinic Foundation Systèmes et méthodes de vitrification de tissu

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991018575A1 (fr) * 1990-06-04 1991-12-12 Biosurface Technology, Inc. Recipient de stockage et de distribution d'un pansement pour lesions dermiques
WO1996024018A1 (fr) * 1995-01-30 1996-08-08 Organogenesis, Inc. Procede et structure d'emballage pour la conservation et le stockage cryogeniques d'equivalents de tissu de culture

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991018575A1 (fr) * 1990-06-04 1991-12-12 Biosurface Technology, Inc. Recipient de stockage et de distribution d'un pansement pour lesions dermiques
WO1996024018A1 (fr) * 1995-01-30 1996-08-08 Organogenesis, Inc. Procede et structure d'emballage pour la conservation et le stockage cryogeniques d'equivalents de tissu de culture

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009155430A2 (fr) * 2008-06-18 2009-12-23 The Cleveland Clinic Foundation Systèmes et méthodes de vitrification de tissu
WO2009155430A3 (fr) * 2008-06-18 2010-12-16 The Cleveland Clinic Foundation Systèmes et méthodes de vitrification de tissu
US8030063B2 (en) 2008-06-18 2011-10-04 The Cleveland Clinic Foundation Systems and methods for vitrifying tissue

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