WO2003018778A3 - Procede de preparation d'une composition lyophilisee contenant des bacteries lactiques a viabilite et activite bacteriennes ameliorees lors d'un stockage a temperature ambiante et composition obtenue - Google Patents

Procede de preparation d'une composition lyophilisee contenant des bacteries lactiques a viabilite et activite bacteriennes ameliorees lors d'un stockage a temperature ambiante et composition obtenue Download PDF

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Publication number
WO2003018778A3
WO2003018778A3 PCT/FR2002/002983 FR0202983W WO03018778A3 WO 2003018778 A3 WO2003018778 A3 WO 2003018778A3 FR 0202983 W FR0202983 W FR 0202983W WO 03018778 A3 WO03018778 A3 WO 03018778A3
Authority
WO
WIPO (PCT)
Prior art keywords
freeze
preparing
lactic acid
acid bacteria
room temperature
Prior art date
Application number
PCT/FR2002/002983
Other languages
English (en)
Other versions
WO2003018778A2 (fr
Inventor
Catherine Beal
Michele Marin
Georges Corrieu
Fernanda Fonseca
Original Assignee
Agronomique Inst Nat Rech
Catherine Beal
Michele Marin
Georges Corrieu
Fernanda Fonseca
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agronomique Inst Nat Rech, Catherine Beal, Michele Marin, Georges Corrieu, Fernanda Fonseca filed Critical Agronomique Inst Nat Rech
Publication of WO2003018778A2 publication Critical patent/WO2003018778A2/fr
Publication of WO2003018778A3 publication Critical patent/WO2003018778A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065—Microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms

Abstract

L'invention concerne un procédé de préparation d'une composition lyophilisée qui comprend les étapes suivantes :a) préparation d'un concentré de bactéries lactiques dans un milieu liquide, ledit milieu liquide comprenant : (i) un composé antioxydant hydrosoluble ou une association de composés antioxydants hydrosolubles ; et (ii) un composé ou une association de composés augmentant la température de transition vitreuse du produit lyophilisé; b) lyophilisation du concentré de bactéries lactiques lioprotégé préparé à l'étape a), selon les étapes de lyophilisation suivantes : (b1) congélation du concentré de bactéries ; (b2) dessication primaire, de préférence dans des conditions opératoires douces ; (b3) dessication secondaire jusqu'à obtenir une composition bactérienne lyophilisée ayant une activité de l'eau d'environ 0,1 et dont la température de transition vitreuse (Tg) est supérieure d'au moins 20°C à la température de stockage (Ts) prédéterminée. Application aux industries biologiques et alimentaires.
PCT/FR2002/002983 2001-08-30 2002-08-30 Procede de preparation d'une composition lyophilisee contenant des bacteries lactiques a viabilite et activite bacteriennes ameliorees lors d'un stockage a temperature ambiante et composition obtenue WO2003018778A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0111269A FR2829147B1 (fr) 2001-08-30 2001-08-30 Procede de preparation d'une composition lyophilisee contenant des bacteries lactiques a viabilite et activite bacteriennes ameliorees lors d'un stockage a temperature ambiante et composition obtenue
FR01/11269 2001-08-30

Publications (2)

Publication Number Publication Date
WO2003018778A2 WO2003018778A2 (fr) 2003-03-06
WO2003018778A3 true WO2003018778A3 (fr) 2003-11-27

Family

ID=8866835

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2002/002983 WO2003018778A2 (fr) 2001-08-30 2002-08-30 Procede de preparation d'une composition lyophilisee contenant des bacteries lactiques a viabilite et activite bacteriennes ameliorees lors d'un stockage a temperature ambiante et composition obtenue

Country Status (2)

Country Link
FR (1) FR2829147B1 (fr)
WO (1) WO2003018778A2 (fr)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2882369B1 (fr) * 2005-02-22 2007-04-20 Air Liquide Procede par lequel on modifie la viabilite d'un microorganisme lyophilise par conditionnement de son milieu de croissance par des gaz
US20080095752A1 (en) * 2006-10-20 2008-04-24 Win-Chin Chiang Method for extending the shelf-life of powdered nutritional formulations which contain viable probiotics
CA2756042A1 (fr) * 2009-03-26 2010-09-30 Moobella, Inc. Compositions alimentaires contenant des probiotiques deshydrates, leurs procedes de fabrication et leurs utilisations
US9210945B2 (en) 2009-07-31 2015-12-15 The Iams Company Animal food having low water activity
US20110027417A1 (en) 2009-07-31 2011-02-03 Patrick Joseph Corrigan Process for Dusting Animal Food
US8691303B2 (en) 2009-07-31 2014-04-08 The Iams Company Dusted animal food
US10104903B2 (en) 2009-07-31 2018-10-23 Mars, Incorporated Animal food and its appearance
US9173423B2 (en) 2009-07-31 2015-11-03 The Iams Company Animal food kibble with electrostatically adhered dusting
EP2464721B1 (fr) 2009-08-14 2015-10-21 DuPont Nutrition Biosciences ApS Micro-organismes déshydratés enrobés présentant une stabilité et une viabilité accrues
FR2954349A1 (fr) 2009-12-22 2011-06-24 Agronomique Inst Nat Rech Sulfatase modifiant selectivement les glycosaminoglycanes
US20130089638A1 (en) * 2011-10-11 2013-04-11 Mead Johnson Nutrition Company Compositions Comprising Maltotriose And Methods Of Using Same To Inhibit Damage Caused By Dehydration Processes
GB201121992D0 (en) 2011-12-21 2012-02-01 Danisco Process
US11304428B2 (en) 2015-02-16 2022-04-19 Mars, Incorporated Interlocking kibble
CN107529789A (zh) 2015-04-28 2018-01-02 马斯公司 制备灭菌湿宠物食品的方法
FR3067928B1 (fr) 2017-06-23 2020-02-28 Fondation Mediterranee Infection Procede de conservation d'un echantillon de bacteries

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3897307A (en) * 1974-10-23 1975-07-29 Hansens Lab Inc Stabilized dry cultures of lactic acid-producing bacteria
EP0259739A1 (fr) * 1986-09-10 1988-03-16 Rhone-Poulenc Inc. Stabilité de cultures lyophilisées

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3897307A (en) * 1974-10-23 1975-07-29 Hansens Lab Inc Stabilized dry cultures of lactic acid-producing bacteria
EP0259739A1 (fr) * 1986-09-10 1988-03-16 Rhone-Poulenc Inc. Stabilité de cultures lyophilisées

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ADAMS ET AL.: "Optimizing the lyophilization cycle and the consequences of collaps on the pharmaceutical acceptability of Erwinia L-asparaginase", JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 8606, no. 12, December 1996 (1996-12-01), pages 1301 - 1305, XP001076679 *
ANDERSEN ET AL.: "Storage stability of freeze-dried starter cultures (Streptococcus thermophilus) as related to physical state of freezing matrix", LEBENSMITTEL-WISSENSCHAFT & TECHNOLOGY, vol. 32, no. 8, 1 December 1999 (1999-12-01), pages 540 - 547, XP002198082 *
BHANDARI ET AL.: "Implication of glass transition for the drying and stability of dried foods", JOURNAL OF FOOD ENGINEERING, vol. 40, 1999, pages 71 - 79, XP002198083 *
FONSECA ET AL.: "State diagrams and sorption isotherm of bacterial suspensions and fermented medium", THERMOCHIMICA ACTA, vol. 366, 2001, pages 167 - 182, XP002198084 *

Also Published As

Publication number Publication date
FR2829147A1 (fr) 2003-03-07
WO2003018778A2 (fr) 2003-03-06
FR2829147B1 (fr) 2003-12-12

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