WO2003016525A1 - Procede de production d'alcool a partir d'amidon - Google Patents

Procede de production d'alcool a partir d'amidon Download PDF

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Publication number
WO2003016525A1
WO2003016525A1 PCT/JP2002/008234 JP0208234W WO03016525A1 WO 2003016525 A1 WO2003016525 A1 WO 2003016525A1 JP 0208234 W JP0208234 W JP 0208234W WO 03016525 A1 WO03016525 A1 WO 03016525A1
Authority
WO
WIPO (PCT)
Prior art keywords
yeast
amylase
glucoamylase
surface layer
starch
Prior art date
Application number
PCT/JP2002/008234
Other languages
English (en)
Japanese (ja)
Other versions
WO2003016525A9 (fr
Inventor
Hideki Fukuda
Akihiko Kondo
Atsuo Tanaka
Mitsuyoshi Ueda
Eiichi Satoh
Original Assignee
Kansai Chemical Engineering Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/JP2002/001980 external-priority patent/WO2003016524A1/fr
Application filed by Kansai Chemical Engineering Co., Ltd. filed Critical Kansai Chemical Engineering Co., Ltd.
Priority to JP2003521834A priority Critical patent/JP4189317B2/ja
Publication of WO2003016525A1 publication Critical patent/WO2003016525A1/fr
Publication of WO2003016525A9 publication Critical patent/WO2003016525A9/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Selon cette invention, un plasmide construit de façon à présenter de la glucoamylase sur sa couche superficielle et à sécréter de l'α-amylase est intégré dans une levure qui fermente ensuite avec de l'amidon pour substrat. Ainsi, un alcool peut être efficacement produit par fermentation directement à partir de l'amidon. Cette levure est supérieure au niveau de sa capacité de fermentation alcoolique à une levure possédant de la glucoamylase et de l'α-amylase sur sa couche superficielle cellulaire, à une levure possédant de l'α-amylase sur sa couche superficielle cellulaire et sécrétant de la glucoamylase, et à une levure possédant de la glucoamylase sur sa couche superficielle cellulaire. De plus, à l'aide de l'α-amylase provenant du Streptococcus bovis, de l'éthanol peut être produit directement à partir d'amidon non cuit. Il est également possible d'utiliser une levure qui présente sur sa couche superficielle cellulaire de la glucoamylase et de l'α-amylase en tant que protéine hybride avec le domaine de fonction d'agglutination d'une protéine de liaison de chaîne glucidique.
PCT/JP2002/008234 2001-08-16 2002-08-12 Procede de production d'alcool a partir d'amidon WO2003016525A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2003521834A JP4189317B2 (ja) 2001-08-16 2002-08-12 デンプンからアルコールを製造する方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2001247134 2001-08-16
JP2001-247134 2001-08-16
JPPCT/JP02/01980 2002-03-04
PCT/JP2002/001980 WO2003016524A1 (fr) 2001-08-16 2002-03-04 Procede de production d'alcool a partir d'amidon

Publications (2)

Publication Number Publication Date
WO2003016525A1 true WO2003016525A1 (fr) 2003-02-27
WO2003016525A9 WO2003016525A9 (fr) 2003-10-16

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/008234 WO2003016525A1 (fr) 2001-08-16 2002-08-12 Procede de production d'alcool a partir d'amidon

Country Status (1)

Country Link
WO (1) WO2003016525A1 (fr)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011092041A (ja) * 2009-10-28 2011-05-12 Kansai Chemical Engineering Co Ltd エタノール生産微生物の連続培養発酵装置
US7960511B2 (en) 2008-04-10 2011-06-14 Kabushiki Kaisha Toyota Chuo Kenkyusho Acid-resistance endoglucanase and the use of thereof
JP2011182644A (ja) * 2010-03-04 2011-09-22 Kaneka Corp グルタチオンの製造方法
JP2011254748A (ja) * 2010-06-08 2011-12-22 Kobe Univ エタノールの生産方法
JP2012095669A (ja) * 2003-10-22 2012-05-24 Keck Graduate Inst 一倍体接合方法を用いた酵母におけるヘテロ多量体のポリペプチド類の合成方法
JP2013142171A (ja) * 2012-01-11 2013-07-22 Nippon Telegr & Teleph Corp <Ntt> 金属の防食方法
JP2013142170A (ja) * 2012-01-11 2013-07-22 Nippon Telegr & Teleph Corp <Ntt> 水素脆化の防止方法
JP2014005500A (ja) * 2012-06-25 2014-01-16 Nippon Telegr & Teleph Corp <Ntt> 金属構造体の防食方法
WO2015033948A1 (fr) * 2013-09-04 2015-03-12 関西化学機械製作株式会社 Procédé de production d'éthanol
KR20210055262A (ko) * 2019-11-07 2021-05-17 강원도 응집성 및 알코올 발효능이 우수한 신규 사카로마이세스 세레비지애 afy-7 및 이를 포함하는 주류

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ATSUMI UEDA ET AL.: "Saibo hyoso display tanpakushitsu no atarashii hatsugenkei", PROTEIN, NUCLEIC ACID AND ENZYME, vol. 46, no. 11, 10 August 2001 (2001-08-10), pages 1480 - 1487, XP002958908 *
JANSE B.J. ET AL.: "One-step enzymatic hydrolysis of starch using a recombinant strain of saccharomyces cerevisiae producing alpha-amylase, glucoamylase and pullulanase", APPL. MICROBIOL. BIOTECHNOL., vol. 42, 1995, pages 878 - 883, XP002958907 *
MURAI T. ET AL.: "Evaluation of the function of arming yeast displaying glucoamylase on its cell surface by direct fermentation of corn to ethanol", JURNAL OF FERMENTATION AND BIOENGINEERING, vol. 86, no. 6, 1998, pages 569 - 572, XP002942427 *
SATOH E. ET AL.: "Molecular cloning and expression of two alpha-amylase genes from streptococcus bovis 148 in escherichia coli", APPL. ENVIRON. MICROBIOL., vol. 59, no. 11, 1993, pages 3669 - 3673, XP002958909 *
WATARI J. ET AL.: "Molecular cloning and analysis of the yeast flocculation gene FL01", YEAST, vol. 10, no. 2, 1994, pages 211 - 225, XP002138551 *

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10259883B2 (en) 2003-10-22 2019-04-16 Keck Graduate Institute Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
US9873746B2 (en) 2003-10-22 2018-01-23 Keck Graduate Institute Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
US11447560B2 (en) 2003-10-22 2022-09-20 Keck Graduate Institute Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
US10155819B2 (en) 2003-10-22 2018-12-18 Alderbio Holdings Llc Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
JP2012095669A (ja) * 2003-10-22 2012-05-24 Keck Graduate Inst 一倍体接合方法を用いた酵母におけるヘテロ多量体のポリペプチド類の合成方法
US7960511B2 (en) 2008-04-10 2011-06-14 Kabushiki Kaisha Toyota Chuo Kenkyusho Acid-resistance endoglucanase and the use of thereof
JP2011092041A (ja) * 2009-10-28 2011-05-12 Kansai Chemical Engineering Co Ltd エタノール生産微生物の連続培養発酵装置
JP2011182644A (ja) * 2010-03-04 2011-09-22 Kaneka Corp グルタチオンの製造方法
JP2011254748A (ja) * 2010-06-08 2011-12-22 Kobe Univ エタノールの生産方法
JP2013142170A (ja) * 2012-01-11 2013-07-22 Nippon Telegr & Teleph Corp <Ntt> 水素脆化の防止方法
JP2013142171A (ja) * 2012-01-11 2013-07-22 Nippon Telegr & Teleph Corp <Ntt> 金属の防食方法
JP2014005500A (ja) * 2012-06-25 2014-01-16 Nippon Telegr & Teleph Corp <Ntt> 金属構造体の防食方法
US9816113B2 (en) 2013-09-04 2017-11-14 Kansai Chemical Engineering Co., Ltd. Method for producing ethanol
WO2015033948A1 (fr) * 2013-09-04 2015-03-12 関西化学機械製作株式会社 Procédé de production d'éthanol
KR20210055262A (ko) * 2019-11-07 2021-05-17 강원도 응집성 및 알코올 발효능이 우수한 신규 사카로마이세스 세레비지애 afy-7 및 이를 포함하는 주류
KR102256699B1 (ko) 2019-11-07 2021-05-27 강원도 응집성 및 알코올 발효능이 우수한 신규 사카로마이세스 세레비지애 afy-7 및 이를 포함하는 주류

Also Published As

Publication number Publication date
WO2003016525A9 (fr) 2003-10-16

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