WO2003016525A1 - Process for producing alcohol from starch - Google Patents

Process for producing alcohol from starch Download PDF

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Publication number
WO2003016525A1
WO2003016525A1 PCT/JP2002/008234 JP0208234W WO03016525A1 WO 2003016525 A1 WO2003016525 A1 WO 2003016525A1 JP 0208234 W JP0208234 W JP 0208234W WO 03016525 A1 WO03016525 A1 WO 03016525A1
Authority
WO
WIPO (PCT)
Prior art keywords
yeast
amylase
glucoamylase
surface layer
starch
Prior art date
Application number
PCT/JP2002/008234
Other languages
French (fr)
Japanese (ja)
Other versions
WO2003016525A9 (en
Inventor
Hideki Fukuda
Akihiko Kondo
Atsuo Tanaka
Mitsuyoshi Ueda
Eiichi Satoh
Original Assignee
Kansai Chemical Engineering Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/JP2002/001980 external-priority patent/WO2003016524A1/en
Application filed by Kansai Chemical Engineering Co., Ltd. filed Critical Kansai Chemical Engineering Co., Ltd.
Priority to JP2003521834A priority Critical patent/JP4189317B2/en
Publication of WO2003016525A1 publication Critical patent/WO2003016525A1/en
Publication of WO2003016525A9 publication Critical patent/WO2003016525A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

A plasmid having been constructed so as to present glucoamylase on its surface layer and secret α-amylase is integrated into a yeast and then the yeast is grown using starch as a substrate. Thus, al alcohol can be efficiently produced by fermentation directly from the starch. This yeast is superior in alcohol fermentability to a yeast presenting glucoamylase and α-amylase on the cell surface layer, a yeast presenting α-amylase on the cell surface layer and secreting glucoamylase, and a yeast presenting glucoamylase on the surface layer. Using α-amylase originating in Streptococcus bovis, moreover, ethanol can be directly produced from uncooked starch. It is also possible to employ a yeast which presents on the cell surface layer glucoamylase and α-amylase as a fused protein with the agglutination function domain of a sugar chain-binding protein.
PCT/JP2002/008234 2001-08-16 2002-08-12 Process for producing alcohol from starch WO2003016525A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2003521834A JP4189317B2 (en) 2001-08-16 2002-08-12 Process for producing alcohol from starch

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2001-247134 2001-08-16
JP2001247134 2001-08-16
JPPCT/JP02/01980 2002-03-04
PCT/JP2002/001980 WO2003016524A1 (en) 2001-08-16 2002-03-04 Process for producing alcohol from starch

Publications (2)

Publication Number Publication Date
WO2003016525A1 true WO2003016525A1 (en) 2003-02-27
WO2003016525A9 WO2003016525A9 (en) 2003-10-16

Family

ID=26345146

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/008234 WO2003016525A1 (en) 2001-08-16 2002-08-12 Process for producing alcohol from starch

Country Status (1)

Country Link
WO (1) WO2003016525A1 (en)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011092041A (en) * 2009-10-28 2011-05-12 Kansai Chemical Engineering Co Ltd Apparatus for continuously culturing and fermenting ethanol-producing microorganism
US7960511B2 (en) 2008-04-10 2011-06-14 Kabushiki Kaisha Toyota Chuo Kenkyusho Acid-resistance endoglucanase and the use of thereof
JP2011182644A (en) * 2010-03-04 2011-09-22 Kaneka Corp Method for producing glutathione
JP2011254748A (en) * 2010-06-08 2011-12-22 Kobe Univ Method for producing ethanol
JP2012095669A (en) * 2003-10-22 2012-05-24 Keck Graduate Inst Method of synthesizing heteromultimeric polypeptide in yeast using haploid mating strategy
JP2013142171A (en) * 2012-01-11 2013-07-22 Nippon Telegr & Teleph Corp <Ntt> Method for preventing corrosion of metal
JP2013142170A (en) * 2012-01-11 2013-07-22 Nippon Telegr & Teleph Corp <Ntt> Method for preventing hydrogen embrittlement
JP2014005500A (en) * 2012-06-25 2014-01-16 Nippon Telegr & Teleph Corp <Ntt> Anticorrosive method for metallic structure
WO2015033948A1 (en) * 2013-09-04 2015-03-12 関西化学機械製作株式会社 Method for producing ethanol
KR20210055262A (en) * 2019-11-07 2021-05-17 강원도 New Saccharomyces cerevisiae strain having high flocculation and fermentation ability and alcoholic beverages comprising the same

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ATSUMI UEDA ET AL.: "Saibo hyoso display tanpakushitsu no atarashii hatsugenkei", PROTEIN, NUCLEIC ACID AND ENZYME, vol. 46, no. 11, 10 August 2001 (2001-08-10), pages 1480 - 1487, XP002958908 *
JANSE B.J. ET AL.: "One-step enzymatic hydrolysis of starch using a recombinant strain of saccharomyces cerevisiae producing alpha-amylase, glucoamylase and pullulanase", APPL. MICROBIOL. BIOTECHNOL., vol. 42, 1995, pages 878 - 883, XP002958907 *
MURAI T. ET AL.: "Evaluation of the function of arming yeast displaying glucoamylase on its cell surface by direct fermentation of corn to ethanol", JURNAL OF FERMENTATION AND BIOENGINEERING, vol. 86, no. 6, 1998, pages 569 - 572, XP002942427 *
SATOH E. ET AL.: "Molecular cloning and expression of two alpha-amylase genes from streptococcus bovis 148 in escherichia coli", APPL. ENVIRON. MICROBIOL., vol. 59, no. 11, 1993, pages 3669 - 3673, XP002958909 *
WATARI J. ET AL.: "Molecular cloning and analysis of the yeast flocculation gene FL01", YEAST, vol. 10, no. 2, 1994, pages 211 - 225, XP002138551 *

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10259883B2 (en) 2003-10-22 2019-04-16 Keck Graduate Institute Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
US9873746B2 (en) 2003-10-22 2018-01-23 Keck Graduate Institute Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
US11447560B2 (en) 2003-10-22 2022-09-20 Keck Graduate Institute Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
US10155819B2 (en) 2003-10-22 2018-12-18 Alderbio Holdings Llc Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
JP2012095669A (en) * 2003-10-22 2012-05-24 Keck Graduate Inst Method of synthesizing heteromultimeric polypeptide in yeast using haploid mating strategy
US7960511B2 (en) 2008-04-10 2011-06-14 Kabushiki Kaisha Toyota Chuo Kenkyusho Acid-resistance endoglucanase and the use of thereof
JP2011092041A (en) * 2009-10-28 2011-05-12 Kansai Chemical Engineering Co Ltd Apparatus for continuously culturing and fermenting ethanol-producing microorganism
JP2011182644A (en) * 2010-03-04 2011-09-22 Kaneka Corp Method for producing glutathione
JP2011254748A (en) * 2010-06-08 2011-12-22 Kobe Univ Method for producing ethanol
JP2013142170A (en) * 2012-01-11 2013-07-22 Nippon Telegr & Teleph Corp <Ntt> Method for preventing hydrogen embrittlement
JP2013142171A (en) * 2012-01-11 2013-07-22 Nippon Telegr & Teleph Corp <Ntt> Method for preventing corrosion of metal
JP2014005500A (en) * 2012-06-25 2014-01-16 Nippon Telegr & Teleph Corp <Ntt> Anticorrosive method for metallic structure
US9816113B2 (en) 2013-09-04 2017-11-14 Kansai Chemical Engineering Co., Ltd. Method for producing ethanol
WO2015033948A1 (en) * 2013-09-04 2015-03-12 関西化学機械製作株式会社 Method for producing ethanol
KR20210055262A (en) * 2019-11-07 2021-05-17 강원도 New Saccharomyces cerevisiae strain having high flocculation and fermentation ability and alcoholic beverages comprising the same
KR102256699B1 (en) 2019-11-07 2021-05-27 강원도 New Saccharomyces cerevisiae strain having high flocculation and fermentation ability and alcoholic beverages comprising the same

Also Published As

Publication number Publication date
WO2003016525A9 (en) 2003-10-16

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