WO2003016524A1 - Process for producing alcohol from starch - Google Patents

Process for producing alcohol from starch Download PDF

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Publication number
WO2003016524A1
WO2003016524A1 PCT/JP2002/001980 JP0201980W WO03016524A1 WO 2003016524 A1 WO2003016524 A1 WO 2003016524A1 JP 0201980 W JP0201980 W JP 0201980W WO 03016524 A1 WO03016524 A1 WO 03016524A1
Authority
WO
WIPO (PCT)
Prior art keywords
yeast
starch
amylase
glucoamylase
surface layer
Prior art date
Application number
PCT/JP2002/001980
Other languages
French (fr)
Japanese (ja)
Inventor
Hideki Fukuda
Akihiko Kondo
Atsuo Tanaka
Mitsuyoshi Ueda
Eiichi Satoh
Original Assignee
Kansai Chemical Engineering Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kansai Chemical Engineering Co., Ltd. filed Critical Kansai Chemical Engineering Co., Ltd.
Priority to JP2003521834A priority Critical patent/JP4189317B2/en
Priority to PCT/JP2002/008234 priority patent/WO2003016525A1/en
Publication of WO2003016524A1 publication Critical patent/WO2003016524A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

An alcohol can be directly and efficiently obtained from starch via fermentation by integrating a plasmid, which is constructed so as to present glucoamylase on the surface layer and secret α-amylase, to a yeast and growing the resultant yeast using starch as a substrate. Compared with a yeast presenting glucoamylase and α-amylase on the cell surface layer, a yeast presenting α-amylase on the cell surface layer and secreting glucoamylase or a yeast presenting glucoamylase on the surface layer, the above-described yeast has an excellent capability of alcohol fermentation and shows an alcohol yield of 40%, i.e., being comparable to alcohol fermentation form glucose. Use of α-amylase originating in Streptococcus bovis makes it possible to produce ethanol directly from uncooked starch (raw starch).
PCT/JP2002/001980 2001-08-16 2002-03-04 Process for producing alcohol from starch WO2003016524A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2003521834A JP4189317B2 (en) 2001-08-16 2002-08-12 Process for producing alcohol from starch
PCT/JP2002/008234 WO2003016525A1 (en) 2001-08-16 2002-08-12 Process for producing alcohol from starch

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001-247134 2001-08-16
JP2001247134 2001-08-16

Publications (1)

Publication Number Publication Date
WO2003016524A1 true WO2003016524A1 (en) 2003-02-27

Family

ID=19076530

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/001980 WO2003016524A1 (en) 2001-08-16 2002-03-04 Process for producing alcohol from starch

Country Status (2)

Country Link
JP (1) JP4189317B2 (en)
WO (1) WO2003016524A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10253337B2 (en) 2016-12-05 2019-04-09 Stellenbosch University Recombinant yeast and use thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05236942A (en) * 1992-02-27 1993-09-17 Tsusho Sangyosho Kiso Sangyo Kyokucho New agglutinative alcohol fermentation yeast

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05236942A (en) * 1992-02-27 1993-09-17 Tsusho Sangyosho Kiso Sangyo Kyokucho New agglutinative alcohol fermentation yeast

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MURAI T. ET AL.: "Evaluation of the function of arming yeast displaying glucoamylase on its cell surface by direct fermentation of corn to ethanol", JOURNAL OF FERMENTATION AND BIOENGINEERING, vol. 86, no. 6, 1998, pages 569 - 572, XP002942427 *
NAKAMURA Y. ET AL.: "Alcohol fermentation of starch by a genetic recombinant yeast having glucoamylase activity", BIOTECHNOLOGY AND BIOENGINEERING, vol. 53, 1997, pages 21 - 25, XP002952402 *
SATOH E. ET AL.: "Molecular cloning and expression of two alpha-amylase genes from streptococcus bovis 148 in escherichia coli", APPL. ENVIRON. MICROBIOL., vol. 59, no. 11, November 1993 (1993-11-01), pages 3669 - 3673, XP002952401 *
TANAKA A. ET AL.: "Cell surface engineering of yeast: construction of arming yeast with biocatalyst", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 90, no. 2, 2000, pages 125 - 136, XP002947291 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10253337B2 (en) 2016-12-05 2019-04-09 Stellenbosch University Recombinant yeast and use thereof

Also Published As

Publication number Publication date
JP4189317B2 (en) 2008-12-03
JPWO2003016525A1 (en) 2005-02-17

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