WO2002068952A1 - Procede d'analyse du polymere a l'aide d'une technique d'abrasion au laser et systeme d'analyse - Google Patents
Procede d'analyse du polymere a l'aide d'une technique d'abrasion au laser et systeme d'analyse Download PDFInfo
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- WO2002068952A1 WO2002068952A1 PCT/JP2002/001594 JP0201594W WO02068952A1 WO 2002068952 A1 WO2002068952 A1 WO 2002068952A1 JP 0201594 W JP0201594 W JP 0201594W WO 02068952 A1 WO02068952 A1 WO 02068952A1
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- polymer
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/10—Ion sources; Ion guns
- H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
- H01J49/161—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission using photoionisation, e.g. by laser
- H01J49/164—Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/44—Resins; rubber; leather
- G01N33/442—Resins, plastics
Definitions
- the present invention relates to a method for analyzing a polymer using a laser abrasion and its system.
- the present invention relates to a method for analyzing a polymer using a laser abrasion and a system thereof, and more particularly, to a method for analyzing a polymer using a laser abrasion capable of significantly improving the efficiency of analysis as compared with the related art.
- molecular analysis methods and systems for example, polymers using laser ablation suitable for mass analysis of various macromolecules such as DNA, protein, RNA, PNA, lipids, and sugars
- the present invention relates to an analysis method and a system thereof. Background art
- mass spectrometry In recent years, the application range of mass spectrometry has rapidly expanded from the fields of physics and chemistry to the fields of life sciences such as medicine and biochemistry. In particular, the development of determination analysis of protein molecular weight and determination analysis of amino acid sequence is remarkable.
- the principle of such mass spectrometry is that a sample is ionized by various methods, ions obtained by ionization are separated according to mass / charge, and the intensity of each separated ion is measured.
- mass spectrometry is performed by adding electrons to the polymer itself and analyzing its mass, or by subdividing high molecular weight molecules into low molecular weight molecular ions. And compared the constituent molecules.
- conventional ion generation methods in polymer mass spectrometry include, for example, secondary ion mass spectrometry (SIMS), in which high-energy atomic ions collide with a polymer to ionize it, and low molecular weight molecules by electron impact.
- SIMS secondary ion mass spectrometry
- An electron ionization (ED) method in which mass analysis is performed by subdividing ions, and a matrix-assisted laser monoionization (MA LDI) method are known.
- the present invention has been made in view of the above-mentioned various problems of the conventional technology, and an object thereof is to provide an atomic ion of a constituent atom constituting a polymer.
- An object of the present invention is to provide a method and a system for mass spectrometry of polymers using an abrasion. More specifically, for example, in the case of performing mass spectrometry, it is necessary to use a laser-absorption method that eliminates the risk of difficulty in angular analysis of the mass spectrum and that does not require a high-resolution mass spectrometer. It is intended to provide a method and system for mass spectrometry of polymers. Another object of the present invention is to make it possible to simultaneously realize atomization and ionization of constituent atoms constituting a polymer with a single laser, thereby greatly simplifying the system configuration. It is an object of the present invention to provide a method for analyzing a polymer using a laser application and a system therefor, which enable the conversion.
- an object of the present invention is to use a laser ablation that enables efficient analysis even in a situation where various kinds of labeled isotopes are mixed, and a high molecular weight polymer. It is intended to provide an analysis method and its system. Disclosure of the invention
- the present invention provides a method for producing various polymers such as DNA, protein, RNAPNA, lipids, and sugars by ultrashort pulse laser light. It generates atomic ions by atomizing molecules, and analyzes the generated atomic ions. As a result, chemical analysis of various polymers can be performed.
- the polymer by polymerizing a polymer with one light of an ultrashort pulse laser, the polymer is decomposed into pieces to atomize each atom constituting the polymer, and at the same time, atomization is performed. This ionizes the dangling atoms into monovalent ions. Quantitative analysis is possible by analyzing the atomic ions generated by the ionization of DNA.
- mass spectrometry of low-mass atomic ions is performed, which not only eliminates the risk of difficulty in analyzing the mass spectrum, but also allows the mass spectrometer to have high resolution. It is not necessary to provide.
- the present invention by ablating a polymer with an ultrashort pulse laser beam, it is possible to simultaneously atomize the polymer and ionize the atomized atoms into monovalent ions. It can be performed efficiently. Therefore, the system configuration can be simplified, and, for example, it is possible to simultaneously use various kinds of labeling elements in chemical analysis, so that the analysis efficiency can be significantly improved.
- atomization and ionization of the labeling element can be simultaneously performed by one ultra-short pulse laser, so that the system configuration can be greatly simplified.
- ionization is ionization (non-resonant ionization) performed by a non-resonant process due to a high peak intensity of an ultra-short pulse laser beam, in a situation where various kinds of labeled isotopes are mixed.
- each labeled atom can be ionized, which makes it easy to apply to multi-labeled systems, and enables highly accurate and highly efficient analysis of macromolecules.
- the present invention is extremely suitable for use in quantitative analysis of gene expression levels, which will become increasingly important in the future.
- the present invention provides a polymer to be analyzed by irradiating the polymer with laser light.
- This is a method for analyzing a polymer using a laser abrasion method, in which a polymer is atomized into constituent elements by abrasion, the atomized constituent elements are ionized, and the ionized constituent elements are analyzed.
- the laser light that irradiates the target polymer and ablates the polymer is an ultrashort pulse laser light, and the ultrashort pulse laser light is applied to the polymer to be analyzed.
- the polymer is atomized into constituent elements and ionized at the same time, and the constituent elements that have been ionized are analyzed.
- examples of the above-mentioned analysis include mass spectrometry, and examples of the analysis other than mass spectrometry include, for example, chemical analysis (ordinary so-called chemical analysis) and optical analysis ( Fluorescence method).
- the polymer to be analyzed may be solid-phased.
- the method of solid-phase immobilization of the polymer is a method including a step of solid-phase immobilizing by dropping a solution of the polymer to be analyzed on a substrate and drying the solution. You may.
- the substrate may be a solid, and the thermal conductivity of the solid may be 0.1 W ⁇ ⁇ ⁇ 1 ⁇ K 1 or more.
- the polymer to be analyzed may be an element-labeled polymer.
- the element label may be a Group 1 element in the periodic table.
- the element label may be a Group 16 element in the periodic table.
- the element label is a group 17 element in the periodic table. You may.
- the element label may be a transition metal element in the periodic table.
- the element label may be a stable isotope label.
- the ultrashort pulse laser beam that irradiates the polymer to be analyzed and irradiates the polymer has a pulse time width of 10 picoseconds or less,
- the value output may be greater than 10 megabits.
- an ultrashort pulse laser beam that irradiates a polymer to be analyzed and prescribes the polymer has a pulse time width of 1 femtosecond or more and 1 picosecond or less.
- the peak value output may be between 1 gigawatt and 10 gigabits or less.
- the analysis of the ionized constituent elements may be mass spectrometry.
- the mass spectrometry may be mass spectrometry based on a time-of-flight method.
- a plurality of ionized constituent elements may be analyzed simultaneously.
- the macromolecule to be analyzed may be a nucleic acid or an analog of the nucleic acid fixed on the DNA microarray.
- DNARNAPNA DNARNAPNA
- the DNA microarray may be a multichannel DNA microarray.
- the short-pulse laser light for ablating the polymer and the polymer to be analyzed are moved by moving at least one of the polymer and the short-pulse laser light for ablating the polymer.
- the analysis may be performed by ablating the high molecule to be analyzed without omission or duplication.
- the present invention also provides a method of irradiating a polymer to be analyzed with a laser beam to ablate the polymer, thereby atomizing the polymer into constituent elements, ionizing the atomized constituent elements, and ionizing the polymer.
- a polymer analysis system using a laser ablation for analyzing the constituent elements comprising: a vacuum chamber in which an evening get can be placed; an analyzer placed in the vacuum chamber; An ultra-short pulse laser that emits a pulsed laser beam and irradiates it to a sunset placed in the vacuum chamber is provided.
- moving means for moving the gate in the vacuum chamber in the evening.
- the moving means for moving the target may be a rotating means for rotating the target.
- a moving means for moving an irradiation position of one light of the ultrashort pulse laser on the evening gate may be further provided.
- the analyzer may be a mass analyzer.
- the mass spectrometer may be a quadrupole mass spectrometer.
- the mass analyzer may be a time-of-flight mass analyzer.
- the mass spectrometer may be an ion cyclotron-type free-transform mass spectrometer.
- the ultrashort pulse laser may emit short pulse laser light having a pulse time width of 10 picoseconds or less and a peak value output of 10 megabytes or more. .
- the ultrashort pulse laser according to the above (3) wherein the pulse time width is from 1 fs to 1 ps, and the peak value output is from 1 gigabit to 10 gigaseconds.
- One light may be applied.
- a polymer when a polymer is ablated by one light of an ultrashort pulse laser, it is sufficient to irradiate one shot (one pulse) of one light of the ultrashort pulse laser to the polymer.
- a polymer may be irradiated with a plurality of shots (multiple pulses) of an ultrashort pulse laser beam, and the number of shots (pulses) of the ultrashort pulsed laser beam applied to the polymer may be appropriately selected. Good.
- the ultrashort pulse laser preferably has a pulse time width of 10 picoseconds or less, and particularly, a laser pulse of 1 femtosecond or more and 1 picosecond or less, which is usually called a femtosecond laser. It is appropriate to use
- the peak value output is preferably 10 megabytes or more, and particularly preferably 1 gigabyte or more and 10 gigabytes or less. If it is larger than this range, multiply-charged ions will be generated, making it difficult to analyze the mass spectrum.If it is below this range, the efficiency of atomization and ionization will decrease, and the atomic ion signal cannot be observed. It is.
- the pulse time width is 110 femtoseconds and the peak value output is 2 gigawatts
- extremely good results can be obtained.
- ionization can be efficiently performed simultaneously with atomization.
- An ultrashort pulse laser beam such as a femtosecond laser beam is irradiated onto a polymer sample labeled with an isotope. Therefore, it is not necessary to selectively ionize the labeling element, and it becomes possible to use various labeling elements.
- the repetition rate of laser irradiation can be increased to several kHz, it is suitable for high-speed analysis.
- a short pulse laser for ablating the polymer is moved by moving at least one of the short pulse laser for ablation of the polymer and the polymer to be analyzed.
- the analysis is performed by ablation of the polymer to be analyzed by one light without omission and duplication. That is, in the present invention, for example, a large number of samples applied over a large area are leaked by moving a spot of a short pulse laser beam and a substrate coated with a polymer to be analyzed as a sample. It is possible to perform abrasion without duplication. This is particularly useful in applications to DNA microarrays.
- the present invention not only makes the analysis speed much faster than in the past, but also makes it possible to simultaneously analyze the expression of genes with extremely low expression levels.
- a gene expression analysis using a DNA microarray there is a gene expression analysis using a DNA microarray, and the analysis can be speeded up. That is, according to the present invention, it is possible to use many types of isotopes as a label. For example, if a stable isotope is used as a label, the type of the label is the number of various types of stable isotopes (27 0 types). This is the conventional labeling method (2 to 6 types) and radioisotopes (about 10 types) can dramatically increase the amount of information.
- a label used in DNA microarray experiments for example, 32 S, which is a stable isotope of group 16 in the periodic table, such as 3 S K and 41 K which are a stable isotope of group 1 in the periodic table, and 35 S, etc., such as group 17 stable isotope der Ru 35 Cl, 37 C1 in the periodic table, a stable isotope of the transition metal in the periodic table 118 Sn, nucleotides containing stable isotopes such 12Q S n Label the probe with.
- the probe is hybridized with the gate nucleic acid on the DNA microarray, the probe is ablated with an ultrashort pulse laser to atomize the molecules, and then, for example, detected by a mass spectrometer.
- the amount of isotope contained in the spilled profile can be determined. Therefore, the amount ratio of the probe can be calculated and obtained.
- the probe was labeled with a fluorescent dye.
- the speed can be increased.
- the expression data of the gene of the DNA microarray is obtained as a relative value to the reference sample.
- a conventional DNA microarray experiment in which only two types of fluorescent labels can be used, it is difficult to compare a large number of samples between experiments.
- multiple channels of three or more types of probes labeled with different elements are mixed, simultaneously hybridized with the evening gate, and multi-channel measurement is performed by the polymer analysis method using laser ablation according to the present invention.
- the present invention provides a method for labeling with a fluorescent dye or a radioisotope. It is applicable to all research fields.
- a stable isotope can be used without using a radioactive isotope as a labeling element.
- the facility used is not limited, and thus the medical facility or a private company can be used. Can also be installed in the city, and its ripple effects are incalculable.
- FIG. 1 is a conceptual configuration explanatory diagram of an example of a configuration of a mass spectrometry system as an example of a polymer analysis system for performing a polymer analysis method using laser ablation according to the present invention.
- Figure 2 is a chart showing the specifications of the two samples (Sample 1 and Sample 2) used in the experiment.
- Figures 3 (a), (b) and (c) are the mass spectra of sample 1 measured by a quadrupole mass analyzer.
- Fig. 3 (a) shows the case where the output of one short pulse laser beam is 230 ⁇ J
- Fig. 3 (b) shows the case where the output of one short pulse laser beam is 53J
- Fig. 3 (c) Shows the case where the output of one short pulse laser beam is 480 / J.
- the sensitivity of the quadrupole mass spectrometer was reduced by two orders of magnitude compared to the measurements in Figs. 3 (a) and 3 (b).
- FIG. 4 is a drawing showing a state in which the sample 1 was peeled off by the irradiation of the ultrashort pulse laser beam, and the state was observed by a microscope.
- FIG. 5 is an explanatory diagram showing the results of measuring the depth and area of a flaw formed in the evening get.
- FIG. 6 is a mass spectrum of Sample 2 measured by a quadrupole mass analyzer.
- FIG. 7 is the mass spectrum of Sample 3 measured by the quadrupole mass analyzer.
- FIG. 8 is a mass spectrum of Sample 4 measured by a quadrupole mass analyzer.
- FIG. 1 is a conceptual diagram illustrating an example of the configuration of a mass spectrometry system as an example of a polymer analysis system for performing a polymer analysis method using a laser abrasion according to the present invention. ing.
- the mass spectrometry system 1 0 includes a 1 0- 8 ⁇ 1 0- 6 T vacuum chamber 1 2 can be set to a vacuum degree of orr, this vacuum chamber 1 evening disposed within 2 one Gedzuto 1 4, vacuum Quadrupole mass spectrometer 16 placed in tank 12, rotation introduction terminal 18 that rotates evening gate 14, and one ultra-short pulse laser beam emitted to illuminate evening gate 14 It has an ultrashort pulse laser 20 and a focus lens 22 for condensing one light of the ultrashort pulse laser emitted from the ultrashort pulse laser 20 onto the evening gate 14.
- the ultrashort pulse laser 120 is composed of a titanium sapphire laser and has the following parameters. That is,
- the quadrupole mass spectrometer 16 is 90 degrees perpendicular to the irradiation direction of the ultrashort pulse laser beam emitted from the ultrashort pulse laser 20 and irradiated to the evening gate 14. is set up.
- the focal length of the focus lens 22 that collects one light of the ultrashort pulse laser emitted from the ultrashort pulse laser 120 is set to, for example, 25 cm.
- sample No. 1 and sample No. 2 with the specifications shown in Fig. 2 were used as samples for the experiment.
- a target 14 was prepared by a spin coating method.
- a substantially square silicon substrate having a side of about 2 cm is prepared, and a concentrated solution of the sample 1 or the sample 2 is dropped on the silicon substrate. Thereafter, the silicon substrate is rotated for 90 seconds by 100 rotations (0). By doing so, the solution of sample 1 or sample 2 dropped onto the silicon substrate spreads, evaporates the solvent while solidifying, and cures while keeping the surface flat. Then, the silicon substrate with the cured Sample 1 or Sample 2 on the surface is further placed in a thermostat at about 120 ° C and left for 30 minutes to 1 hour.
- the material of the substrate need not be a semiconductor, but may be a metal or an insulator.
- a substrate with high thermal conductivity provides higher ion detection efficiency.
- a solid is used as the substrate, and the solid used as the substrate preferably has a thermal conductivity of 0.1 W ′ m ⁇ 1 ⁇ K ⁇ 1 or more.
- the ultrashort pulse laser beam emitted from the ultrashort pulse laser Use the lens 22 to focus the light on the evening get 14 and ablate the sample 1 or sample 2 formed on the evening get 14.
- the pulse width of one light of the ultrashort pulse laser emitted from the ultrashort pulse laser 20 was 110 femtoseconds, and the output was changed to 53 J, 23 O JLLJ, 480 / J.
- the quadrupole mass analyzer 16 measures the mass of monovalent ions generated by irradiating the target 14 with an ultrashort pulse laser beam.
- FIGS. 3 (a), 3 (b) and 3 (c) show the mass spectrum of the sample 1 measured by the quadrupole mass analyzer 16 by the above-described method.
- the polymer of Sample 1 was atomized by the ablation using an ultrashort pulse laser such as a femtosecond laser, and was ionized simultaneously with the atomization.
- FIG. 4 shows a micrograph showing the state of the evening target 14 from which the sample 1 has been peeled off by irradiation with one light of an ultrashort pulse laser.
- Fig. 4 the white circle inside the concentric double circle and the black circle around it are visible. Recognized. This one spot corresponds to the irradiation of the shirt in the evening for 8 ms. In other words, it was stripped off by about 8 shots of ultra-short pulse laser light.
- FIG. 5 shows the results of measuring the depth and area of the flaw formed on the get 14 in the evening.
- the depth level B (L v. B) is considered to be the surface of the silicon substrate, and sample 1 which was inside a cylinder with a depth of 8 ⁇ m and a width of 224 It is recognized that the silicon in the cone was stripped off with a pulse of 8 shots. From this, when the amount of the sample and the amount of silicon stripped by one shot of the ultrashort pulse laser beam are estimated, the following results are obtained.
- Amount of sample stripped in one shot of ultrashort pulse laser light is amount of sample stripped in one shot of ultrashort pulse laser light
- Amount of silicon removed in one shot of ultrashort pulse laser is amount of silicon removed in one shot of ultrashort pulse laser
- the ultrashort pulse laser beam (specifically, a single femtosecond laser with a pulse time width of 110 femtoseconds) atomizes macromolecules (about 500 molecular weight). It was confirmed that ionization was possible. C Further, it can be said that the isotope of P can be used as a label. From the above, by coating a polymer at a high density on a silicon substrate, the constituent elements in organic molecules, such as C, N, 0, Na, F, and P, are exposed to ultrashort pulse laser light.
- the evening gate 14 with the sample 3 cured on the surface as described above is mounted in the vacuum chamber 12 and the vacuum chamber 12 is evacuated, and the degree of vacuum in the vacuum chamber 12 is 10— e To rr or less.
- the ultra-short pulse laser beam having the above-mentioned parameters emitted from the ultra-short pulse laser 20 is condensed on the evening gate 14 using the focus lens 22, and the evening gate 14 Abbreviation
- the evening gate 14 By rotating the evening gate 14 by the rotation introducing terminal 18, the evening gate 14 is abbreviated into a spot without any omission. Further, at this time, by opening and closing the shirt while moving the focus lens 22, it is possible to form the spot on the evening gate 14 in a spot-like manner without any overlap and overlap.
- the mass of monovalent ions generated by irradiating the ultra-short pulse laser beam to the evening gate 14 is measured by the quadrupole mass analyzer 16.
- FIG. 7 shows an example of the mass spectrum of the sample measured by the quadrupole mass analyzer 16 by the above-described method.
- sample 4 a DNA sample containing a Group 17 element and a C1-substituted DNA sample
- the pulse time width of the ultrashort pulse laser 20 was 110 femtoseconds and the peak value output was The experimental results when setting to 2 GW are described.
- the experimental site for Samples 1 and 2 As before, before conducting mass spectrometry of the polymer by the mass spectrometry system 10, first, as a sample 14, the polymer to be subjected to mass spectrometry (the C 1 -substituted DNA sample described above) ) Is dissolved in a solvent and applied to a silicon substrate, and the silicon substrate is left in a thermostat at 50 degrees Celsius for about 30 minutes to evaporate the solvent applied to the silicon substrate. Prepare what you have.
- the evening gate 14 on which the sample 4 was hardened on the surface as described above was mounted in the vacuum chamber 12, and the inside of the vacuum chamber 12 was evacuated to reduce the degree of vacuum in the vacuum chamber 12. 1 0—Set to be less than 6 Torr.
- an ultra-short pulse laser beam having the above-described parameters emitted from the ultra-short pulse laser 120 is condensed on the evening target 14 using the focus lens 22, and Ablate gate 14
- the evening gate 14 is leaked, and it is abbreviated in a spot shape without duplication.
- the mass of monovalent ions generated by irradiating the ultra-short pulse laser beam to the evening gate 14 is measured by the quadrupole mass analyzer 16.
- FIG. 8 shows an example of the mass spectrum of the sample measured by the quadrupole mass spectrometer 16 by the above-described method.
- the method for analyzing a polymer using the laser ablation according to the present invention can be used, for example, for mass analysis of various polymers such as DNA, protein, RNA, PNA, lipid, and sugar. It is.
- a polymer sample itself labeled with an isotope element can be ionized at the atomic level, and the labeled element can be detected.
- an isotope can be used as a label for DNA, and the number of kinds of labels can be increased to, for example, 270, which is the number of stable isotopes. This can dramatically increase the amount of information compared to conventional labeling methods such as the fluorescence method (2 types) and radioisotopes (about 10 types).
- a quadrupole mass spectrometer is used as the mass spectrometer.
- the present invention is not limited to this, and by measuring the flight time of atoms, it is obvious.
- mass spectrometry of multiple atoms can be performed simultaneously with one laser irradiation.
- mass spectrometry of a plurality of atoms can be performed simultaneously.
- mass spectrometry has been described as a method for analyzing a polymer, but it is needless to say that the present invention is not limited to this, and the present invention may be used for analysis other than mass spectrometry. It may be.
- the rotation introducing terminal 18 for rotating the target 14 is used as the moving means for moving the target 14, but it is needless to say that the present invention is not limited to this.
- an appropriate moving means such as a movable table on which the evening gate 14 can be placed may be used.
- the evening gate 14 is rotated using the rotation introduction terminal 18 so that the evening gate 14 can be abreast without omission and duplication. Needless to say, it is not limited to this. Ultra-short pulse lasers are provided with a means of moving the irradiation position of the light to the evening getter. You may make it chill. Industrial applicability
- the present invention is configured as described above, the atomic ion of the constituent atoms constituting the polymer is generated, and the generated atomic ion is analyzed using a laser breaker.
- the present invention has an excellent effect of providing a method and system for mass analysis of a polymer using a laser ablation which does not require a high-resolution analyzer, which is an analysis method and a system thereof.
- a high-resolution analyzer which is an analysis method and a system thereof.
- mass spectrometry it is possible to eliminate the possibility that it is difficult to parse the mass spectrum and to eliminate the need for a high-resolution mass spectrometer. It has excellent effects.
- the present invention since the present invention is configured as described above, it has an excellent effect that an efficient analysis can be performed even in a situation where various kinds of labeled isotopes are mixed.
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
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US10/469,160 US6815672B2 (en) | 2001-02-27 | 2002-02-22 | Method of analyzing polymer by using laser abrasion and system therefor |
EP02700701A EP1376120A1 (en) | 2001-02-27 | 2002-02-22 | Method of analyzing polymer by using laser abrasion and system therefor |
CA002439481A CA2439481A1 (en) | 2001-02-27 | 2002-02-22 | Method of analyzing polymer by using laser abrasion and system therefor |
AU2002233692A AU2002233692B2 (en) | 2001-02-27 | 2002-02-22 | Method of analyzing polymer by using laser abrasion and system therefor |
Applications Claiming Priority (4)
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JP2001-51919 | 2001-02-27 | ||
JP2001051919 | 2001-02-27 | ||
JP2002-44340 | 2002-02-21 | ||
JP2002044340A JP3640387B2 (ja) | 2001-02-27 | 2002-02-21 | レーザーアブレーションを用いた高分子の分析方法およびそのシステム |
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WO2002068952A1 true WO2002068952A1 (fr) | 2002-09-06 |
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PCT/JP2002/001594 WO2002068952A1 (fr) | 2001-02-27 | 2002-02-22 | Procede d'analyse du polymere a l'aide d'une technique d'abrasion au laser et systeme d'analyse |
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US (1) | US6815672B2 (ja) |
EP (1) | EP1376120A1 (ja) |
JP (1) | JP3640387B2 (ja) |
AU (1) | AU2002233692B2 (ja) |
CA (1) | CA2439481A1 (ja) |
TW (1) | TWI243901B (ja) |
WO (1) | WO2002068952A1 (ja) |
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JP2005517459A (ja) * | 2002-02-19 | 2005-06-16 | ユニバーシティ・オブ・ブリストル | スクリーニング方法 |
WO2004019026A1 (ja) * | 2002-08-26 | 2004-03-04 | Yoshihide Hayashizaki | レーザーアブレーションを用いたタンパク質の分析方法 |
JPWO2004019026A1 (ja) * | 2002-08-26 | 2005-12-15 | 林崎 良英 | レーザーアブレーションを用いたタンパク質の分析方法 |
WO2004081555A1 (ja) * | 2003-03-14 | 2004-09-23 | Nec Corporation | 質量分析システムおよび分析方法 |
JPWO2004081555A1 (ja) * | 2003-03-14 | 2006-06-15 | 日本電気株式会社 | 質量分析システムおよび分析方法 |
US7586091B2 (en) | 2003-03-14 | 2009-09-08 | Nec Corporation | Mass spectrometric system and mass spectrometry |
WO2005074003A1 (ja) * | 2004-01-28 | 2005-08-11 | Kyoto University | レーザ分析装置及び方法 |
JPWO2005074003A1 (ja) * | 2004-01-28 | 2007-09-13 | 国立大学法人京都大学 | レーザ分析装置及び方法 |
Also Published As
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CA2439481A1 (en) | 2002-09-06 |
US6815672B2 (en) | 2004-11-09 |
EP1376120A1 (en) | 2004-01-02 |
JP2002328114A (ja) | 2002-11-15 |
JP3640387B2 (ja) | 2005-04-20 |
TWI243901B (en) | 2005-11-21 |
US20040113606A1 (en) | 2004-06-17 |
AU2002233692B2 (en) | 2006-07-27 |
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