WO2002046360A2 - Procede de separation de l'agent de la tuberculose dans un milieu de culture, milieu de culture destine a la separation de l'agent de la tuberculose, procede de fabrication de ce milieu et stimulateur de la croissance de l'agent de la tuberculose - Google Patents

Procede de separation de l'agent de la tuberculose dans un milieu de culture, milieu de culture destine a la separation de l'agent de la tuberculose, procede de fabrication de ce milieu et stimulateur de la croissance de l'agent de la tuberculose Download PDF

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Publication number
WO2002046360A2
WO2002046360A2 PCT/UA2001/000041 UA0100041W WO0246360A2 WO 2002046360 A2 WO2002046360 A2 WO 2002046360A2 UA 0100041 W UA0100041 W UA 0100041W WO 0246360 A2 WO0246360 A2 WO 0246360A2
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WO
WIPO (PCT)
Prior art keywords
tuberculosis
causative agent
producing
πiτaτelnοy
mass
Prior art date
Application number
PCT/UA2001/000041
Other languages
English (en)
Russian (ru)
Other versions
WO2002046360A3 (fr
Inventor
Vladimir Vasiljevich Vlasenko
Petr Ivanovich Bagriy
Original Assignee
Vladimir Vasiljevich Vlasenko
Petr Ivanovich Bagriy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vladimir Vasiljevich Vlasenko, Petr Ivanovich Bagriy filed Critical Vladimir Vasiljevich Vlasenko
Priority to EA200300549A priority Critical patent/EA005527B1/ru
Priority to AU2002225587A priority patent/AU2002225587A1/en
Publication of WO2002046360A2 publication Critical patent/WO2002046360A2/fr
Publication of WO2002046360A3 publication Critical patent/WO2002046360A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Definitions

  • the invention relates to medical and veterinary medicine and may be used in research and development and medical laboratories.
  • the stimulus of the causative agent of tuberculosis which is a short-circuit of chemical compounds, is known to have been inoculated with oxygen, because of the The use of known stimuli does not ensure a sufficient acceleration of the incidence of tuberculosis.
  • Such a method makes it possible to expedite the method of research, to improve safety conditions, but it is not long enough.
  • the main task of the invention was to create a new stimulus for growth and a new healthy environment using this stimulus, and also
  • P ⁇ s ⁇ avlennaya task ⁇ eshae ⁇ sya ⁇ em, ch ⁇ s ⁇ glasn ⁇ iz ⁇ b ⁇ e ⁇ eniyu zayavlyae ⁇ sya s ⁇ imulya ⁇ ⁇ s ⁇ a v ⁇ zbudi ⁇ elya ⁇ ube ⁇ uleza, s ⁇ de ⁇ zhaschy ⁇ imiches ⁇ ie s ⁇ edineniya in s ⁇ s ⁇ av ⁇ y ⁇ v ⁇ dya ⁇ elemen ⁇ y: na ⁇ y, ⁇ isl ⁇ d, v ⁇ d ⁇ d, ugle ⁇ d, ⁇ y ⁇ a ⁇ a ⁇ e ⁇ izue ⁇ sya following s ⁇ s ⁇ av ⁇ m ing ⁇ edien ⁇ v, wt.%:
  • the claimed stimulus contains distilled or bidistilled water.
  • the stimulus of growth can additionally contain 0.1-0.9 wt.% Calcium carbonate.
  • the stimulus for the invention is as agreed to also contain 0.01- 0.4 wt.% Of the size of the feed and 01-0.02 wt. % glucose is harmless.
  • the food environment is consonant with the invention as a stimulant of the calcium-dependent enzymes that consumes the calcium and the non-volatile propellant.
  • the content of the aminogroup in nitrogen in the finished food medium is at least 1, 8%.
  • the nutritional base is diluted under special conditions in sterile cups with a layer of 6-8 mm.
  • the posed problem is also solved by the allocation of the pathogen to tuberculosis in a healthy environment, which is free of charge, and it is in the process of receiving a good food.
  • the food environment is designed for efficient use.
  • the stimulus of the pathogen exciter of the following composition was secured: secerose x / h 3.0 g of sulfuric acid 2.0 the disinfectant is two-placed 0.005 g of distilled water up to 1000 ml ⁇ 7.2 ⁇ 0.2.
  • the bulk of the incentive stimulus . and the growth of the causative agent of tuberculosis and the nutritional basis is 1: 20.
  • the nutritional environment is as follows.
  • Settled humidity is not more than 7.0%.
  • the content of the administrative unit in the finished healthy environment is 1.87%.
  • the method of separation is based on non-blocking. freeware eminogrupp ⁇ and tritruppy clicking on the equivalent of a large group of strong groups. ⁇ echello and the end of ⁇ Seg. Vania shares potential.
  • Calcium-independent stimulant (calcium chloride) 0.12 g
  • the stimulus of the pathogen of tuberculosis 100 ml.
  • the mass ratio of stimulation of growth and nutritional basis is 1: 25
  • the content of the aminogroup of nitrogen in the finished food environment is 1, 85%.
  • the mass supply of the incentive and the healthy base is 1: 21.
  • the main source of food is 85%.
  • the source of the research is% of the guerromatic research methods. 1 unit ⁇ . ⁇ . Bi ⁇ ge ⁇ a. - 3rd ed. lavishInterpretation and Interpretation. ⁇ ., Medicine, 1982, p. 79 - 80

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

L'invention peut s'utiliser dans des laboratoires de recherche et de fabrication de profil vétérinaire ou médical. Le stimulateur de l'agent de la tuberculose contient des composés chimiques qui comprennent les éléments suivants: sodium, oxygène, hydrogène, carbone et qui contiennent (en % en poids) 2,0 - 5,0 de saccharose chimiquement pur, 0,1 - 0,9 d'alcool rectifié, 0,05 - 0,15 de bicarbonate de sodium, 0,005 - 0,9 de pyrocitrate de sodium disubstitué et un diluant (jusqu'à 1000 ml). Le stimulateur de croissance peut en outre contenir de 0,1 à 0,9 % en pds de chlorure de calcium et entre 0,01 et 0,4 % en poids d'amidon de pommes de terre ainsi qu'entre 0,01 et 0,02 % en pds de glucose anhydre. Le milieu de culture destiné à la séparation de l'agent de la tuberculose sous forme de solution aqueuse contient ce qui suit (en % en pds): peptone sèche par fermentation - 5-7, agar-agar ou agar microbiologique - 1,4-1,6, amidon de pommes de terre - 1,377 - 1,577, stimulateur de ferments dépendants de calcium - 0,012-0,014, carbonate de sodium - 0,005-0,015, stimulateur de la croissance de l'agent de la tuberculose - 10-18 et diluant - jusqu'à 100. Le procédé de fabrication du milieu de culture et le procédé de séparation de l'agent de la tuberculose dans ce milieu de culture sont également présentés. L'invention permet d'accélérer les recherches biologiques tout en assurant une sensibilité élevée du procédé.
PCT/UA2001/000041 2000-11-03 2001-11-02 Procede de separation de l'agent de la tuberculose dans un milieu de culture, milieu de culture destine a la separation de l'agent de la tuberculose, procede de fabrication de ce milieu et stimulateur de la croissance de l'agent de la tuberculose WO2002046360A2 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EA200300549A EA005527B1 (ru) 2000-11-03 2001-11-02 Стимулятор роста возбудителя туберкулеза, питательная среда для выделения возбудителя туберкулеза, способ получения питательной среды, способ выделения возбудителя туберкулеза
AU2002225587A AU2002225587A1 (en) 2000-11-03 2001-11-02 Methods for producing tuberculosis causative agent on a culture medium, culture medium for producing the tuberculosis causative agent, method for producing said medium and growth stimulator for the tuberculosis causative agent

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
UA2000116242 2000-11-03
UA2000116242A UA43467C2 (uk) 2000-11-03 2000-11-03 Стимулятор росту збудника туберкульозу "рідин" (варіанти), живильне середовище для виділення збудника туберкульозу (варіанти), спосіб одержання живильного середовища (варіанти), спосіб виділення збудника туберкульозу на живильному середовищі (варіанти)

Publications (2)

Publication Number Publication Date
WO2002046360A2 true WO2002046360A2 (fr) 2002-06-13
WO2002046360A3 WO2002046360A3 (fr) 2002-09-26

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PCT/UA2001/000041 WO2002046360A2 (fr) 2000-11-03 2001-11-02 Procede de separation de l'agent de la tuberculose dans un milieu de culture, milieu de culture destine a la separation de l'agent de la tuberculose, procede de fabrication de ce milieu et stimulateur de la croissance de l'agent de la tuberculose

Country Status (4)

Country Link
AU (1) AU2002225587A1 (fr)
EA (1) EA005527B1 (fr)
UA (1) UA43467C2 (fr)
WO (1) WO2002046360A2 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2989003A (en) * 1958-09-25 1961-06-20 Cav Ltd Liquid fuel pumps for internal combustion engines
CN1103893A (zh) * 1993-12-14 1995-06-21 中国科学院上海植物生理研究所 分枝杆菌干燥培养基及其生产方法
RU2099417C1 (ru) * 1993-01-06 1997-12-20 Центральный научно-исследовательский институт туберкулеза РАМН Питательная среда с пиразинамидом для определения лекарственной устойчивости возбудителей туберкулеза и их идентификации
RU2106879C1 (ru) * 1995-10-03 1998-03-20 Научно-исследовательский институт по изучению лепры Минздравмедпрома РФ Способ культивирования медленнорастущих микобактерий
RU2117046C1 (ru) * 1993-03-09 1998-08-10 Санкт-Петербургский научно-исследовательский институт фтизиопульмонологии Питательная среда для ускоренного выделения возбудителя туберкулеза из очагов внелегочной локализации на основе среды финна-ii

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5558092A (en) * 1978-10-27 1980-04-30 Mitsui Toatsu Chem Inc Method of culturing tubercle bacillus

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2989003A (en) * 1958-09-25 1961-06-20 Cav Ltd Liquid fuel pumps for internal combustion engines
RU2099417C1 (ru) * 1993-01-06 1997-12-20 Центральный научно-исследовательский институт туберкулеза РАМН Питательная среда с пиразинамидом для определения лекарственной устойчивости возбудителей туберкулеза и их идентификации
RU2117046C1 (ru) * 1993-03-09 1998-08-10 Санкт-Петербургский научно-исследовательский институт фтизиопульмонологии Питательная среда для ускоренного выделения возбудителя туберкулеза из очагов внелегочной локализации на основе среды финна-ii
CN1103893A (zh) * 1993-12-14 1995-06-21 中国科学院上海植物生理研究所 分枝杆菌干燥培养基及其生产方法
RU2106879C1 (ru) * 1995-10-03 1998-03-20 Научно-исследовательский институт по изучению лепры Минздравмедпрома РФ Способ культивирования медленнорастущих микобактерий

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BIRGERA M.O.: 'Spravochnik po mikrobiologicheskim i virusologicheskim metodam issledovaliya' MEDITSINA 1982, MOSCOW, pages 79 - 80 *

Also Published As

Publication number Publication date
UA43467C2 (uk) 2001-12-17
EA005527B1 (ru) 2005-04-28
AU2002225587A1 (en) 2002-06-18
EA200300549A1 (ru) 2003-10-30
WO2002046360A3 (fr) 2002-09-26

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