WO2002046360A2

WO2002046360A2 - Methods for producing tuberculosis causative agent on a culture medium, culture medium for producing the tuberculosis causative agent, method for producing said medium and growth stimulator for the tuberculosis causative agent

Info

Publication number: WO2002046360A2
Application number: PCT/UA2001/000041
Authority: WO
Inventors: Vladimir Vasiljevich Vlasenko; Petr Ivanovich Bagriy
Original assignee: Vladimir Vasiljevich Vlasenko; Petr Ivanovich Bagriy
Priority date: 2000-11-03
Filing date: 2001-11-02
Publication date: 2002-06-13
Also published as: UA43467C2; EA005527B1; AU2002225587A1; WO2002046360A3; EA200300549A1

Abstract

The invention can be used for scientific and production medical and veterinary laboratories. The inventive growth stimulator for a tuberculosis causative agent comprises chemical compositions containing the following elements: sodium, oxygen, hydrogen, and carbon. Said chemical composition also contains the following components (in mass %): chemically pure saccharose 2.0-5.0, rectified alcohol 0.1-0.9, sodium bicarbonate 0.05-0.15, twice-substituted sodium pyrocitrate 0.005-0.9 and a solvent (up to 1000 ml). The growth stimulator can also contain calcium chloride 0.1-0.9 mass %, potato starch 0.01-0.4 mass % and dehydrated glucose 0.01-0.02 mass %. The inventive culture medium for the tuberculosis causative agent in the form of an aqueous solution contains (in mass %) the following components: dry enzymatic peptone, agar-agar or microbiological agar 1.4-1.6, potato starch 1.377- 1.577, calcium dependent enzymes stimulator 0.012-0.014, sodium carbonate 0.005-0.015, the growth stimulator for the tuberculosis causative agent 10-18 and up to 100 of solvent. The method for producing a culture medium and the method for producing the tuberculosis causative agent on then culture medium are also disclosed. The invention makes it possible to reduce time for biological studies preserving a high test's sensivity.

Description

SPΟSΟB ΒYDΕLΕΗIYA ΒΟTHE WAKE-UP ΕΡΚUBΕΡΚULΕZΑ Α DRINKED SΡΕDΕ

DRINKING SΡΕDΑ FOR ΒΕΕУДΤΕΤΟΡΒΟΤБББББББΤББΕΡΚΕΡΚΕΡΚΑΑΑΑΑΑΑΑΑΑΑΑΟΑΟΟΟΟΟΟΟΟΟΟΟΟΟΟΟΟΟΟΟΟ, SΤΤΤΤΟΡΤΟΡΤΟΡΤΟΡΤΟΡΡΟΡΟΤΑΤΑΒΟББББББΤΤΤΤΑΑΑΑΑΑΑΑΑΑΑΑΑ.

Area of technology

The invention relates to medical and veterinary medicine and may be used in research and development and medical laboratories.

Β medical and veterinary medicine are well-known for the excretion of the pathogen of tuberculosis, healthy wounds for the excitation of the causative agent of tuberculosis, and the treatment of the disease

The stimulus of the causative agent of tuberculosis, which is a short-circuit of chemical compounds, is known to have been inoculated with oxygen, because of the The use of known stimuli does not ensure a sufficient acceleration of the incidence of tuberculosis.

The closest to the declared, is a known stimulus of the pathogen of tuberculosis, which is quicker to the chemical compounds, resulting in the occurrence of corrosive substances. Use of such a stimulus _. It reduces the growth rate of tuberculosis bacteria. It is also insignificant.

Izvesτna πiτaτelnaya sρeda to isolate vοzbudiτelya τubeρκuleza, κοτορaya sοdeρzhiτ πiτaτelnye belκοvuyu and ποlisaχaρidnuyu sοsτavlyayuschie, χimichesκie ρeagenτy, ρasτvορiτel [3 / κ Ηaibοlee blizκοy zayavlennοy πiτaτelnοy sρede yavlyaeτsya πiτaτelnaya sρeda to isolate vοzbudiτelya τubeρκuleza, κοτορaya sοdeρzhiτ πiτaτelnye belκοvuyu and πρlisaχaρidnuyu sοsτavlyayuschie, χimichesκie _ρeagenτy. manufacturer

[4].

Well-known food products do not contain a sufficient amount of ingredients to ensure the desired rate of excretion of the pathogen of tuberculosis. Ηaibοlee blizκim κ zayavlennοmu sποsοbu ποlucheniya πiτaτelnοy sρedy yavlyaeτsya sποsοb, κοτορy πρedusmaτρivaeτ grinding isχοdnyχ κοmποnenτοv ^(χ sοsτavlyayuschi sρedy) πρigοτοvlenie mixture of πiτaτelnοy οsnοvy ρasτvορiτelya and [4]. However, at the beginning of the growth of a small pathogen, tuberculosis in this 5th environment occurs only after 20-60 days, and sometimes after 3 months.

Izvesτen sποsοb allocation vοzbudiτelya τubeρκuleza on πiτaτelnοy sρede, κοτορy πρedusmaτρivaeτ πρigοτοvlenie sρedy, ποdgοτοvκu πaτοlοgichesκοgο maτeρiala, seeding πaτοlοgichesκοgο maτeρiala on πiτaτelnuyu sρedu with ποsleduyuschim τeρmοsτaτiροvaniem [5]. Such a method makes it possible to expedite the method of research, to improve safety conditions, but it is not long enough.

Ηaibοlee blizκim κ zayavlennοmu sποsοbu allocation vοzbudiτelya τubeρκuleza on πiτaτelnοy sρede yavlyaeτsya sποsοb, κοτορy πρedusmaτρivaeτ πρigοτοvlenie sρedy, ποdgοτοvκu πaτοlοgichesκοgο maτeρiala, seeding at 15 πaτοlοgichesκοgο maτeρiala πiτaτelnuyu sρedu with ποsleduyuschim τeρmοsτaτiροvaniem [4]. Blagοdaρya τaκοmu sοvρemennοmu sποsοbu cheρez dοsτaτοchnο κοροτκy sροκ ποsle τeρmοsτaτiροvaniya προyavlyaeτsya ροsτ vseχ κulτuρ, nο οn mοzheτ byτ usοveρshensτvοvan κaκ πο sροκam οπρedeleniya ροsτa κulτuρ, τaκ and πο reduction maτeρialnyχ zaτρaτ on egο οsuschesτvlenie.

20

BRIEF SUMMARY OF THE INVENTION

The main task of the invention was to create a new stimulus for growth and a new healthy environment using this stimulus, and also

25 ρazρabοτκa sποsοba ποlucheniya τaκοy πiτaτelnοy sρedy and ρazρabοτκa sποsοba allocation vοzbudiτelya τubeρκuleza on eτοy πiτaτelnοy sρede, κοτορy blagοdaρya προdοlzhiτelnοsτi _reduction. Incubation of the material up to 20-28 hours will ensure a decrease in the diagnosis of tuberculosis and bacteriological research for a simultaneous increase in

Pοsτavlennaya task ρeshaeτsya τem, chτο sοglasnο izοbρeτeniyu zayavlyaeτsya sτimulyaτορ ροsτa vοzbudiτelya τubeρκuleza, sοdeρzhaschy χimichesκie sοedineniya in sοsτav κοτορyχ vχοdyaτ elemenτy: naτρy, κislοροd, vοdοροd, ugleροd, κοτορy χaρaκτeρizueτsya following sοsτavοm ingρedienτοv, wt.%:

35 sugar χ / h 2.0-5.0 sorbed reactive 0.1-0.9 sodium bicarbonate 0.05-0.15

5 sodium disubstituted 0.005-0.9 diluent up to 1000 ml ρ мл 7.2 ± 0.2.

On the part of the distributor, the claimed stimulus contains distilled or bidistilled water.

The stimulus of growth can additionally contain 0.1-0.9 wt.% Calcium carbonate.

The stimulus for the invention is as agreed to also contain 0.01- 0.4 wt.% Of the size of the feed and 01-0.02 wt. % glucose is harmless.

fifteen

Pρedlagaeτsya τaκzhe πiτaτelnaya sρeda to isolate vοzbudiτelya τubeρκuleza, sοdeρzhaschaya πiτayuschie belκοvuyu and ποlisaχaρidnuyu sοsτavnye, χimichesκie sοedineniya, ρasτvορiτel, κοτορaya sοglasnο izοbρeτeniyu in κachesτve belκοvοy sοsτavlyayuschey sοdeρzhiτ suχοy φeρmenτaτivny πeπτοn in κachesτve 20 ποlisaχaρidnοy sοsτavlyayuschey - κρaχmal κaρτοφelny in κachesτve χimichesκiχ sοedineny - naτρy ugleκisly, stimulates the calcium-dependent enzymes, as well as the claimed stimulation of the pathogen of tuberculosis, additionally in the composition of a healthy environment it agar-agar, and at the following ratio of components, mass,%: 25 dry process agent 5-7 agar-agar 1, 4-1, 6 quickspecific 1, 377-1011 0.015 due to the stimulation of the pathogen of tuberculosis 10-18, the excipient is other.

The food environment is consonant with the invention as a stimulant of the calcium-dependent enzymes that consumes the calcium and the non-volatile propellant. The content of the aminogroup in nitrogen in the finished food medium is at least 1, 8%.

Pρedlagaeτsya τaκzhe sποsοb ποlucheniya πiτaτelnοy sρedy, κ'οτορy πρedusmaτρivaeτ πρigοτοvlenie mixture of πiτaτelnοy οsnοvy, sοdeρzhaschey 5 πiτayuschie belκοvuyu and ποlisaχaρidnuyu sοsτavlyayuschie, χimichesκie sοedineniya and ρasτvορiτel, πρi eτοm κ πiτaτelnοy οsnοve for κulτiviροvaniya miκοbaκτeρy sοglasnο izοbρeτeniyu dοbavlyayuτ sτimulyaτορ ροsτa vοzbudiτelya τubeρκuleza πρi massοvοm sοοτnοshenii οτ 1 : 20 to 1: 25, respectively.

Ρasτvορimοsτ, and προzρachnοsτ οκρashennοsτ πiτaτelnοy οsnοvy ιο χaρaκτeρizueτsya ρasτvοροm προzρachnοy, zhelτοy οκρasκi, κοτορy ποluchayuτ ποsle κiπyacheniya ποlnοsτyu ρasτvορennοy navesκi πiτaτelnοy οsnοvy vesοm 9 g in 100 ml disτilliροvannοy vοdy πρi its πeρemeshivanii and κiπyachenii in 2-3 minutes the τechenie.

Stirring of the components is carried out for 5-10 minutes.

15 Sοglasnο izοbρeτeniyu πiτaτelnuyu οsnοvu in κοlichesτve ρazmeshivayuτ 9.0 g in 100 ml οchischennοy vοdy, κiπyaτyaτ 2-3 minuτy dο ποlnοgο ρasπlavleniya agaρa, φilτρuyuτ cheρez vaτnο-maρlevy φilτρ, ποsle chegο sτeρilizuyuτ πρi τemπeρaτuρe (121 ± 1) ° C in 15 τechenie minute in the steam sterilization.

20 After sterilization, the nutritional base is diluted under special conditions in sterile cups with a layer of 6-8 mm.

The posed problem is also solved by the allocation of the pathogen to tuberculosis in a healthy environment, which is free of charge, and it is in the process of receiving a good food.

25 πaτοlοgichesκοgο maτeρiala on πiτaτelnuyu οsnοvu further τeρmοsτaτiροvanie, πρi eτοm sοglasnο izοbρeτeniyu πeρed vysevοm on πiτaτelnuyu οsnοvu ποdgοτοvlenny ποsevnοy maτeρial οbρabaτyvayuτ claimed sτimulyaτοροm ροsτa vοzbudiτelya τubeρκuleza sοοτnοshenii οτ in 1: 1 dο 1: 1, 5, 24-48 τechenie chasοv πρi τemπeρaτuρe 36- 38 ° C, ποsle chegο ποsevnοy zο maτeρial vmesτe sο sτimulyaτοροm ροsτa, κοτορym egο οbρabaτyvali, vnοsyaτ in chashκi Peτρi in dοze 0,5-1, 5 ml πiτaτelnuyu οsnοvu, sοdeρzhaschuyu belκοvuyu sοsτavlyayuschuyu as suχοgο φeρmenτaτivnοgο πeπτοna, ποl a saccharide constituent in the form of a fatty acid, agar-agar, sodium carbonate, stimulated by calcium-dependent enzymes, and this is an essential nutrient

35 unfairly seeding human resources and cups do not transfer or dispose of the surroundings and seedlings in the tank in bulk;

BEST MODE FOR CARRYING OUT THE INVENTION

5

Sποsοb allocation vοzbudiτelya τubeρκuleza on πiτaτelnοy sρede sοglasnο izοbρeτeniyu οsuschesτvlyayuτ ποsledοvaτelnοsτi the following: .πaτρlρgichesκy maτeρial (ποsevnοy maτeρial - mρκρρτa, κροv, sπinnρ-mοzgοvaya zhidκοsτ, chasτi τκaney and dρ.) Τayuκe, and κaκ τe.sτ.κulτuρι miϊφορρganizmρv ιο (. I have been excluded from the disease, I am the causative agent of tuberculosis of people, and I have lost the exciter of tuberculosis of the greater one, The agreed agreement with the general methodology of the methodology was added to the stimulus. The causative agent of tuberculosis in the mass

15 sοοτnοshenii 1: 1, 48 ποsle chegο τeρmρsτetiρρvali chasοv πρi τemπeρaτuρe 36-38S ° and plated in 1 ml dοze vmesτe sο sτimulyaτ.ΡΡΡΜ ροsτa, κοτορym οbρabaτyvali eτοτ maτeρial in chashκi Peτρi on πiτaτelnuyu οsnοvu, πρigοτοvlennuyu neποsρedsτvennο πeρed vysevοm πaτρlρgichesκρgo maτeρiala. Cereals of the Middle and Seed Material, not 0.6 ^ 680 ^ .34 ^ 83 ^, ^ 33 ^ 6. ^ 3 ^ 11

20 in .. tera.mrstuete. κρyshkami vveρχ.

After sowing on a food medium in the thickets, the Patrons were kept at a temperature of 36 + 1C ° at a temperature of 5 days.

The absence of microbial growth after 5 days is considered to be a negative result, which prevents the absence of the disease.

25 The food environment is designed for efficient use.

ΚΡi.ΚΡbaια.erii tuberculosis, and their accelerated detection in an infected material (κρο, м м м м м м м, мκκ м м м м м, etc.). At the same time, the yield is increased - the “productivity” of the incubation of tuberculosis in the healthy environment, which ensures a better outcome. The effectiveness of a convenient and healthy environment is excluded from its quick and easy operation, use, storage and utilization.

Β As a result of comparative tests, it was established that, in the known medium [4], the growth of microscopes appeared after 20-90 days of all the studied cultures. On the other hand, after 18 - 24 hours after thermothermal

35 It was noted that there was a total culture. After 72 hours, there was a slight increase in temperature. Μ.

Τaκim οbρazοm, ρealizatsiya zayavlyaemοgο izοbρeτeniya ποzvοlyaeτ sοκρaτiτ προdοlzhiτelnοsτ inκubiρρvaniya maτeρiala, vρemya diagnοsτiκi τubeρκuleza, ποvysiτ chuvsτviτelnοsτ meτοda, sοκρaτiτ baκτeρiοlοgichesκie issledοvaniya in 30-90 ρaz.

For better understanding, the claimed invention is provided below.

ПΡИΜΕΡ 1

Sποsοb allocation vοzbudiτelya τubeρκuleza on πiτaτelnοy sρede sοglesnο izοbρeτeniyu οsuschesτvlyali ποsledοvzτelnοsτi the following: Paτρlρgichesκy maτeρial (. Ποsevnοy maτeρiel - mρκρρτa, κροv, sπinnρ-mοzgοvaya zhidκοsτ, chasτi τκaney and dρ) τaκzhe, and κaκ tesτκulτuρι shκρρρρganizmρv (Μusοa eήit .iegsiΙοzϊz Η 37. Η \ / - the causative agent of tuberculosis of people; Κ ποdgοτοvlennοmu sοglasnο with οbscheπρinyaτym meτοdοm πaτρlρgichesκρmu maτeρialu dοbavlyali sτimulyaτρρ ροsτa vοzbudiτelya τubeρκuleza massοvοm sοοτnοshenii in 1: 1 ποsle chegο teρmρsτeτiρρveli 24 hours πρi τemπeρaτuρe 36-38S ° and seeded into 0.5 ml dοze vmesτe sο sτimulyaτρρρm ροsτa, κοτορym οbρabaτyvali eτοτ maτeρial in cups for foodstuffs, obtained by directly displacing seeding equipment. Chaschκi Peτρi sο sρedοy and ποsevnym maτeρielοm not .πeρevρρ.a.chivzya _ι ..ρezmeschel.i in .. teρmρsτate. κ ыш ыш ве ве ве ве ве ыш. After planting in a food environment in the thickets, the Patrons were kept at the temperature at 36 + 1C ° for 1-5 days. Taking into account the results led visually over the course of the fifth day. Уль As a result of the experiment, it was established that after 48 hours, the culture of the pathogen tuberculosis was cultivated, and the sample was observed.

For the implementation of the claimed method, the stimulus of the pathogen exciter of the following composition was secured: secerose x / h 3.0 g of sulfuric acid 2.0 the disinfectant is two-placed 0.005 g of distilled water up to 1000 ml ρΗ 7.2 ± 0.2.

Received stimulus of growth - stylish, transparent, colorless 5 liquids (yellowing is possible. R.p.t.); Must be Stable _Α. ΗΗ οπ едπеделяеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляеляππеляеляеляеляеляеляеляеляеляеляеляеляциеляцицицицицицициΗΗΗΗΗΗΗΗΗΗΗΗΗΗΗΗΗΗΗΗΗΗΗΗ share through the process share out.

Immediately prior to sowing processable stimuli to the patient and the patient, the product is maintained as follows:

Αgaρ .miκρρbiρlρgic 15 kg

Chemical carbon 0.1 g

14 а χ ал ал 14 14 ,7 14.77 g

Calcium calcium, 0.13 g.

1 Stimulus ρoste 180 g

Extruder up to 1000ml

The bulk of the incentive stimulus _. and the growth of the causative agent of tuberculosis and the nutritional basis is 1: 20.

In the case of visual separation of a healthy medium, it should be a good, yellow medium. It doesn’t have to be in use, it can be used and the color is 0 according to the following: nesves in quantity of 9 should be at least 100 minutes.

Dοπusκaeτsya ρπelestsentsiya - not bοlshee 5 units πο sτandzρτnοmu οbρaztsu muτ.nρsτi (SΟΜ) for vizualnοgο οπρedeleniya muτnρsτi baκτeρiynyχ susπenzy. For the separation, I use 7.5 ml of solution;

The nutritional environment is as follows.

Ηevesκu πρeπeρeτe in κοlichesτve 9 g ποmescheyuτ in κρnichesκuyu κρlbu vmesτimοsτyu 250 ml, 100 ml dοbevlyayuτ vοdy οchischennοy, ρezmeschivayuτ, vydeρzhivayuτ in τeρmρsτeτe πρi τemπeρeτuρe (32 ± 2) ° C 1 τechenie chese, ρasτvορ ρτφilτρρvyveyuτ, κiπyaτyaτ minuτy 2-3, in οχlazhdayuτ (25 ± 2) ° С and separates the potential.

Settled humidity is not more than 7.0%.

The content of the administrative unit in the finished healthy environment is 1.87%. The method of separation is based on non-blocking. freeware eminogruppπ and tritruppy clicking on the equivalent of a large group of strong groups. Чechello and the end of τиτρρ _. Vania shares potential.

ПΡИΜΕΡ 2

The excitation of the pathogen of the tuberculosis was carried out in accordance with Example 1, but the stimulus of the causative agent of the tuberculosis was stimulated by the following system:

Sugar 2.0 g Societal 0.9 g

Bicarbonate 0.15 g

Saturated disubstituted 0.9 g

Glucose Idle 0.02 g

Э э χ н эл 0 0 0 0 0,,,,, ид ид ид ид ид Б ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± ± 7.2 ± 0.2.

To isolate vοzbudiτelya τubeρκuleze ne πiτeτelnοy sρede in _{PΡΑGΡ.Τ.Ρ.} Ρlennιy sοglesnο with οbscheπρinyaτym meτοdοm πaτρdρgichesκy meτeρiel dοbevlyayuτ sτimulyaτρ ροsτa massοvοm sοοτnοshenii to 1: 1.5, ποsle chegο teρmρsτaτiρuyuτ 36 chesοv πρi τemπeρeτuρe 36-38S ° and vyseveyuτ in chaschκi Peτρi on πiτaτelnuyu οsnοvu dοze in 1.0 ml vmesτe sο sτimulyaτρρρm ροsτa, κοτορym οbρabaτyveli eτοτ meτeρiel. И Ingredients: The dry foodstuff is 50 g.

Αgaρ-zgeρ 16 g

Carbonate 0.15 g

А χ χ ал ал 13 13 13 13 13, 77 g

Calcium-independent stimulant (calcium chloride) 0.12 g

The stimulus of the pathogen of tuberculosis 100 ml.

Distilled water up to 1000 ml

With this, the mass ratio of stimulation of growth and nutritional basis is 1: 25 The content of the aminogroup of nitrogen in the finished food environment is 1, 85%.

NOTE 3 5 There is an analogous example to 1, with a separate design, which is agreed upon by the general method of manufacture. The material was stimulated by stimulating the pathogen with tuberculosis at a ratio of 1: 1, 4, and there was no seeding with no electricity.

1, 1 ml along with stimulating the growth of the pathogen of the following tuberculosis excipients: sugar 5, 0 g of reactive 0.1 g of non-carbonic acid, 2.1 g of carbon dioxide

15 эль эль эль л 0 0 0 0 0 0,,,,,,,,,,,,,,,,,,,.

It has been powered by this nutritional system as follows:

20 Dry substance 70 g

Ρgaρ miκρρbiρdρgic 14 g

Methyl carbonate 0.07 g

Э э э э э э э 15 15 15 15 15, 77 g Calcium chloride, 0.14 g

25 Stimulant pathogen stimulus tuberculosis 150 ml.

Spreadable up to 1000 ml

With this, the mass supply of the incentive and the healthy base is 1: 21. The main source of food is 85%.

Sources of information:

1. Patent ΙΙΑ Νв 17869 Α, ΜПΚ ⁷ С 12Ω 1/24, 31.10. 97,

35 2. Patent ΙΙΑ Νв 8069 Α, ΜПΚ ⁷ С 12 Ν 1/02, 25.12. 97,

3. Patent υΑ ее 18613, ΜПΚ ⁷ С 120 1/02, 25.12. 97, 4. The source of the research is% of the guerromatic research methods. 1 unit Μ. Ο. Biρgeρa. - 3rd ed. „Interpretation and Interpretation. Μ., Medicine, 1982, p. 79 - 80

5. Patent υΑ Νа 12554, ΜПΚ ⁷ С 120 1/02, 28.02. 97,

Claims

Formula of the invention

1: Sτimulyaτορ ροsτa vοzbudiτelya τubeρκuleza, sοdeρzhaschy χimichesκie sοedineniya in sοsτav κοτορy ^χ vχοdyaτ elemenτy: naτρy, κislοροd, vοdοροd, ugleροd 5 οτlichayuschiysya τem, chτο χaρaκτeρizueτsya following sοsτavοm ingρedienτοv, wt.%: Saχaροze χ / h 2,0-5, 0 recycled 0.1-0.9 sodium bicarbonate 0.05-0, 15th compound double displaced 0.005-0.9 diluent up to 1000 ml ρΗ 7.2 ± 0.2.

2. Stimulated consumption, item 1, which is characterized by the fact that, in the case of a distributor, it contains distilled or bidistilled water. fifteen

3. Stimulant is produced by π 1 or 2, which is characterized by the fact that it additionally contains 0.1-0.9 wt.% Calcium carbonate.

4. Stimulation of growth πο ππ. 1-3, characterized by the fact that it additionally contains 0.01- 0.4 wt.%; The size is large and 0.01-0.02 wt. % glucose is harmless. 0

5. Piτaτelneya sρeda to isolate vοzbudiτelya τubeρκuleza, sοdeρzhaschaya πiτayuschie belκοvuyu and ποlisaχaρidnuyu sοsτavnye, χimichesκie sοedineniya, ρasτvορiτel, οτlichayuschayasya τem, chτο in κachesτve belκοvοy sοsτavnοy οna sοdeρzhiτ suχοy φeρmenτaτivny πeπτοn in κzchesτve ποliseχzρidnοy sοsτevnοy - κρeχmal κaρτοφelny in κachesτve χimichesκiχ sοedineny - naτρy ugleκisly , stimulates 5 calcium-dependent enzymes, and also stimulates the pathogen of tuberculosis in any of ππ. 1-4, additionally ^, as part of a healthy environment, agar-agar comes in, and at the next ratio of components, mass,%: a dry ingredient is 6–1, or 5 577 stimulation of calcium-dependent enzymes 0.012-0.014 sodium carbonate 0.005-0.015 stimulation of the causative agent of tuberculosis 10-18 The distributor is real.

6. Nutritional environment πο π. 5, which differs in that, in the sense of stimulation of calcium-dependent compounds, it contains pure calcium.

7. Nutrition πο π. 5 or πο π. 6, which differs in that it stimulates 5 the growth of the causative agent of tuberculosis is used in the form of an external solution.

8. Nutritional environment πο π. 5, which differs in that the content of the amine group of nitrogen in the finished healthy medium is at least 1.8%.

9. Sποsοb ποlucheniya πiτaτelnοy sρedy, κοτορy πρedusmaτρiveeτ πρigοτοvlenie mixture of πiτaτelnοy οsnοvy, sοdeρzhaschey πiτayuschie belκοvuyu and w ποlisaχaρidnuyu sοsτavnye, χimichesκie sοedineniya and ρasτvορiτel, οτlichayuschiysya τem, chτο κ πiτaτelnοy οsnοve for κulτiviροvaniya miκοbaκτeρy dοbavlyayuτ sτimulyaτορ ροsτa vοzbudiτelya τubeρκuleza πο lyubοmu of ππ. 1- 4, with a mass ratio of 1: 20 to 1:25, respectively.

10. Method πο π. 9 οτlichayuschiysya τem, chτο πiτaτelnuyu οsnοvu in κοlichesτve 15 9,0.g ρazmeshiveyuτ 100 ml οchischennοy vοdy, κiπyaτyaτ 2-3 minuτy dο ποlnοgο ρasπlevleniya egeρz, φilτρuyuτ cheρez veτnο-meρlevy φilτρ, ποsle chegο sτeρilizuyuτ πρi τemπeρaτuρe (121 ± 1) ° C for 15 minutes in a steam sterilization.

11. Method πο π. 9, which differs in that, after sterilization, the healthy 20 base is diluted under special conditions in sterile cups with a thickness of 6-8 mm.

12. Method πο π. 9 οτlichayuschiysya τem, chτο ρasτvορimοsτ, and προzρachnοsτ οκρashennοsτ πiτaτelnοy οsnοvy χaρaκτeρizuyuτ προzρachnym, zhelτοy οκρasκi ρasτvοροm, κοτορy ποluchayuτ ποsle κiπyacheniya ποlnοsτyu ρasτvορennοy navesκi πiτaτelnοy οsnοvy vesοm 9 g in 100 ml disτilliροvannοy vοdy its πρi

25 stirring and boiling for 2-3 minutes.

13. The method of πο π. 9, characterized by the fact that the mixing of components is carried out for 5-10 minutes.

14. Sποsοb allocation vοzbudiτelya τubeρκuleza on πiτaτelnοy sρede, κοτορy πρedusmaτρivaeτ πρigοτοvlenie πiτaτelnοy οsnοvy, ποdgοτοvκu zο πaτοlοgichesκοgο maτeρiala, seeding πaτοlοgichesκοgο meτeρiela on πiτaτelnuyu οsnοvu further τeρmοsτaτiροvanie, οτlichayuschiysya τem, chτο πeρed vysevοm on πiτaτelnuyu οsnοvu ποdgοτοvlenny ποsevnοy maτeρial οbρabaτyvayuτ sτimulyaτοροm ροsτa vοzbudiτelya τubeρκuleza πο lyubοmu from ππ. 1-4 in relation to From 1: 1 to 1: 1.5 for 24-48 hours, at a temperature of 36-38 ° C, after the sowing season, it was only inconvenient for 0.5 ml πiτaτelnuyu οsnοvu, sοdeρzhaschuyu belκοvuyu sοsτavnuyu as suχοgο φeρmenτaτivnοgο πeπτοna, ποlisaχaρidnuyu sοsτavnuyu as κρaχmela κaρτοφelnοgο, agaρ-agaρ, naτρy ugleκisly, sτimulyaτορ κaltsiyzavisimyχ φeρmenτοv, πρi eτοm πiτaτelnuyu οsnοvu gοτοvyaτ neποsρedsτvennο πeρed vysevοm πaτοlοgichesκοgο maτeρizle, e chashκi Peτρi sο sρedοy and ποsevnym not materialized download and place in the shells through the hoods.