CN109628321A - It is a kind of to utilize stevia rebaudiana dregs solid fermentation trichoderma strain and preparation method thereof - Google Patents
It is a kind of to utilize stevia rebaudiana dregs solid fermentation trichoderma strain and preparation method thereof Download PDFInfo
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- 241000223259 Trichoderma Species 0.000 title claims abstract description 90
- 238000000855 fermentation Methods 0.000 title claims abstract description 69
- 230000004151 fermentation Effects 0.000 title claims abstract description 69
- 244000228451 Stevia rebaudiana Species 0.000 title claims abstract description 51
- 235000006092 Stevia rebaudiana Nutrition 0.000 title claims abstract description 51
- 239000007787 solid Substances 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 47
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 36
- 235000009566 rice Nutrition 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 30
- 230000001954 sterilising effect Effects 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 17
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 16
- 150000001413 amino acids Chemical class 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 5
- 239000008399 tap water Substances 0.000 claims abstract description 5
- 235000020679 tap water Nutrition 0.000 claims abstract description 5
- 238000001816 cooling Methods 0.000 claims abstract description 4
- 240000007594 Oryza sativa Species 0.000 claims abstract 3
- 238000012545 processing Methods 0.000 claims description 21
- 235000001014 amino acid Nutrition 0.000 claims description 14
- 239000003895 organic fertilizer Substances 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 8
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 3
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 1
- 229910021529 ammonia Inorganic materials 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 9
- 238000011081 inoculation Methods 0.000 abstract description 5
- 230000001737 promoting effect Effects 0.000 abstract description 3
- 241000209094 Oryza Species 0.000 description 34
- 239000002054 inoculum Substances 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 6
- 239000000843 powder Substances 0.000 description 6
- 239000010902 straw Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 235000015099 wheat brans Nutrition 0.000 description 5
- 230000000630 rising effect Effects 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 238000009776 industrial production Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000011343 solid material Substances 0.000 description 3
- -1 stevia glucoside Chemical class 0.000 description 3
- 239000002023 wood Substances 0.000 description 3
- 241000544066 Stevia Species 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229930182478 glucoside Natural products 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000004382 potting Methods 0.000 description 2
- 239000002893 slag Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 244000198134 Agave sisalana Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- CZMRCDWAGMRECN-UHFFFAOYSA-N Rohrzucker Natural products OCC1OC(CO)(OC2OC(CO)C(O)C(O)C2O)C(O)C1O CZMRCDWAGMRECN-UHFFFAOYSA-N 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 229940121375 antifungal agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009837 dry grinding Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The present invention provides a kind of using stevia rebaudiana dregs solid fermentation trichoderma strain and preparation method thereof, belongs to bioengineering field.Formula used is+50% rice bran of 50% stevia rebaudiana dregs, adjusts material pH to 2.5-3.0 by acidolysis amino acid, tap water adjusts water content to 60%.Sterilizing and inoculation technique are 115 DEG C of solid sterilization tank and sterilize 40 minutes, are seeded to 10% trichoderma liquid spawn in sterilization tank material from strain liquid fermentor using culture transferring pipeline after material is cooling, are mixed strain and material using sterilization tank stirring system.Shallow tray fermentation condition is material thickness 3cm, and 26-28 DEG C of temperature, humidity 70-90%, ten thousand grades of toilets are cultivated 4 days, and reesei spores quantity reaches dry weight 1.75 × 1010cfu/g.The application of machine fertilizer is combined with using the trichoderma strain that this method produces, and significant growgh promoting effects are all had to crop.
Description
Technical field
The invention belongs to bioengineering fields, are related to a kind of technology using stevia rebaudiana dregs solid fermentation trichoderma strain.
Background technique
Fungus Trichoderma (Trichoderma, hereinafter referred to as trichoderma) is a kind of important soil inhabitant, spore germination
And mycelia growth can adapt to wider soil temperature and humidity and pH range, and rely on hyperparasitism, antibiosis, Competition
The good biological and ecological methods to prevent plant disease, pests, and erosion effect generated with number of mechanisms such as induction of resistance has become biological antifungal preparation and bio-feritlizer is important
Development object.
A large number of studies show that comparing liquid fermentation, solid fermentation can be significant due to the habit closer to trichoderma strain
Improve trichoderma strain spore count amount.Trichoderma strain solid fermentation be related to the Liquid Culture of trichoderma strain, solid material selection with
Processing, the control of culture environment.Currently, there are some scholars to do some researchs to trichoderma strain solid fermentation, such as China
Patent CN105237146A, which is disclosed, utilizes sisal dregs solid fermentation trichoderma；CN103468629A is disclosed to be sent out using orange peel solid
Ferment trichoderma；CN104059860A, which is disclosed, utilizes wheat bran and wood sawdust solid fermentation trichoderma；CN1055862779A, which is disclosed, utilizes work
Object stalk solid fermentation trichoderma.The trichoderma strain fermentation technique of above-mentioned report is mainly concerned with raw material and feedstock processing, culture temperature
Degree, incubation time etc., but do not account for the yeasting of trichoderma strain, if yeasting be not it is clean, be difficult to protect
The trichoderma strain of card every batch of is attained by qualified quality.
STEVIA REBAUDIANA (Stevia rebaudiana) stevia glucoside rich in is after sucrose and beet sugar
Three kinds of natural sugar sources.Stevia rebaudiana dregs are the products after stevia glucoside is extracted, crude fibre and crude protein rich in.Mesh
The preceding method with stevia rebaudiana dregs production trichoderma strain has not been reported, and this patent is main raw material solid fermentation wood with stevia rebaudiana dregs
Mould agent can not only obtain good trichoderma strain, and can effectively solve the resource utilization problem of stevia rebaudiana dregs.Separately
Outside, this patent to the sterilization method of solid material, the aseptic inoculation method of trichoderma liquid spawn, trichoderma strain shallow tray fermentation ring
Border cleanliness etc. is all described in detail, and has important directive significance to trichoderma strain large-scale industrial production.
Summary of the invention
This research main purpose is in view of the deficiencies of the prior art, to propose and utilize stevia rebaudiana dregs solid fermentation trichoderma strain
Method；Another object is that obtaining good trichoderma strain, which can further apply biological organic fertilizer for this research
It prepares.
The purpose of the present invention can be achieved through the following technical solutions:
A method of trichoderma strain being prepared using stevia rebaudiana dregs solid fermentation, 10-15% trichoderma liquid spawn is added
Shallow tray fermentation material after sterilizing is uniformly mixed, 26-28 DEG C of shallow tray fermentation temperature, humidity 70-90%, ten thousand grades of toilet's cultures 4
It；The shallow tray fermentation material is to be adjusted using the mixture of stevia rebaudiana dregs and rice bran as raw material by the way that acidolysis amino acid is added
Material pH to 2.5-3.0, tap water are adjusted obtained by water content to 60-65%.
The shallow tray fermentation material passes through acidolysis amino acid tune preferably using+50% rice bran of 50% stevia rebaudiana dregs as raw material
Material pH to 2.5-3.0 is saved, tap water adjusts water content to 60% gained.
The method is preferred: shallow tray fermentation material is sterilized 40 minutes using 115 DEG C of solid sterilization tank, is used and is moved after cooling
10% trichoderma liquid spawn is seeded in sterilization tank material by kind pipeline from strain liquid fermentor, utilizes sterilization tank stirring system
Strain and material are mixed.Solid sterilization tank configures mixing plant, on the one hand material can adequately be gone out by stirring
Bacterium；On the other hand 30 DEG C are cooled to after material to pass through after culture transferring pipeline accesses liquid spawn and can sufficiently stir strain and material
It mixes uniformly.
Shallow tray fermentation material thickness preferred 2-4cm, further preferred 2-3cm, still more preferably 3cm.
The stevia rebaudiana dregs are that for water content less than 10%, partial size is less than 5mm after drying and processing.
Free aminoacid content is 15% in the acidolysis amino acid, and hydrogen ion concentration of dissociating is 3M.
The preparation method of the acidolysis amino acid is referring to the patent of invention authorized before applicant: a kind of to utilize sick dead pig
The Liquid amino acid compound of protein production and its application (ZL201410042218.3).
The trichoderma solid spawn prepared according to the method described in the present invention, wherein reesei spores quantity reaches dry weight 1.75
×1010cfu/g。
Beneficial effect utilizes stevia rebaudiana dregs solid fermentation trichoderma strain and preparation method thereof the present invention provides a kind of.It should
Method more comprehensively, is especially described in detail in the methods of sterilizing, inoculation and culture, therefore compared to reported technology
It is more suitable for the large-scale industrial production of trichoderma strain.
Product has the advantages that compared with product currently on the market
1) not only limited to using STEVIA REBAUDIANA solid fermentation trichoderma strain and (utilize the originals such as stalk in surface of material production spore
Material usually only surface produces spore, and spore quantity is in fresh weight 2 × 109Cfu/g or so), therefore the trichoderma strain spore of this method production
Sub- density is high.
2) trichoderma strain produced by the invention is to carry out in toilet, thus the dirt of miscellaneous bacteria can be effectively avoided
Dye, therefore the trichoderma strain spore of this method production is high-quality.
3) test result shows in corn pot experiment, and the application of this product cooperation organic fertilizer can promote significantly jade
The growth of rice plant.
Detailed description of the invention
Fig. 1 utilizes STEVIA REBAUDIANA fermentation trichoderma strain sectional drawing.
Influence of Fig. 2 difference solid material carrier to trichoderma strain solid fermentation.
Fig. 3 STEVIA REBAUDIANA adds influence of the different rice bran ratios to trichoderma strain solid fermentation.
Influence of Fig. 4 difference shallow tray fermentation thickness to trichoderma strain solid fermentation.
Influence of Fig. 5 difference shallow tray fermentation water content to trichoderma strain solid fermentation.
Influence of Fig. 6 difference shallow tray fermentation inoculum concentration to trichoderma strain solid fermentation.
Influence of Fig. 7 difference shallow tray fermentation time to trichoderma strain solid fermentation.
Potting growth-promoting effect picture of Fig. 8 trichoderma strain to corn crop.
Specific embodiment
1, the acquisition of strain and raw material
Strain: Guizhou trichoderma NJAU 4742 (Trichoderma guizhou NJAU 4742, deposit number CGMCC
NO.12166 it) is saved by resource and environmental science institute, Agricultural University Of Nanjing plant nutrient seminar laboratory.
Fermentation raw material: acidolysis amino acid, stevia rebaudiana dregs, rice bran, wheat bran, rice chaff, rice straw powder.Acidolysis amino acid is by river
Yin Lian industry Biotechnology Co., Ltd's friendship provides, free aminoacid content 15%, and hydrogen ion concentration of dissociating is 3M, according to
The method preparation recorded in ZL201410042218.3；Stevia rebaudiana dregs are purchased from East Platform Na Shengte Biotechnology Co., Ltd, through drying
The processing of 10 mesh screens is crossed in dry grinding；Rice bran, wheat bran, rice chaff, rice straw powder are obtained by market purchasing.Stevia rebaudiana dregs, rice bran, bran
The basic physical and chemical of skin, rice chaff and rice straw powder is as shown in table 1:
1 different material physical and chemical property determining of table
2, trichoderma strain solid fermentation
2.1 trichoderma strain Liquid Cultures
Guizhou trichoderma NJAU 4742 is seeded to PDA liquid medium after the activation of PDA solid medium, is passed through
170r/min, under the conditions of 28 DEG C after liquid fermentation and culture 4d culture transferring to liquid fermentation tank in the 4 days acquisition Guizhou trichoderma NJAU that ferment
4742 liquid bacterium solutions.
The screening of 2.2 solid mediums
5 processing, respectively 100% stevia rebaudiana dregs ,+30% rice bran of 70% stevia rebaudiana dregs, 70% are arranged in solid medium
+ 30% rice straw powder of+30% wheat bran of stevia rebaudiana dregs ,+30% rice chaff of 70% stevia rebaudiana dregs and 70% stevia rebaudiana dregs.Guizhou trichoderma NJAU
The 4742 most suitable initial pH of solid fermentation are 2.5-3.0, and adjusting above-mentioned 5 processing pH using acidolysis aminoacids solution is that 2.5-3.0 (adds
The ratio for entering acidolysis amino acid is that 6.6%), adjusting water content is 60%.Inoculation 10% is above-mentioned after 115 DEG C of sterilizings cooling in 40 minutes
The 4742 liquid bacterium solution of Guizhou trichoderma NJAU of acquisition, 28 DEG C of temperature, tray (material thickness 3cm) solid under 90% environment of humidity
Fermentation 4 days.Reesei spores quantity statistics: taking 10g trichoderma solid culture strain, dilute in 90ml sterile water, mixed using being vortexed
Clutch mixes well, and measures spore concentration using blood counting chamber method.As shown in Fig. 2, what different material combination fermented to trichoderma
Spore count measurer has significant impact.The rice bran ferment effect of stevia rebaudiana dregs addition 30% is significantly better than other processing, spore count
Amount reaches 3.5 × 109cfu/g FW.Pure stevia rebaudiana dregs processing spore quantity reaches 1.5 × 109Cfu/g FW shows pure STEVIA REBAUDIANA
Slag is also applied for trichoderma strain solid fermentation.Compared to the processing of pure stevia rebaudiana dregs, stevia rebaudiana dregs add 30% rice chaff and 30% rice straw
Powder does not have significant impact to trichoderma strain spore count, and stevia rebaudiana dregs add 30% wheat bran and significantly reduce trichoderma strain spore
Quantity.The above results show that rice bran, rice chaff and rice straw powder can be used as the carrier of stevia rebaudiana dregs solid fermentation trichoderma strain, addition
Rice bran effect is optimal.
2.3 solid medium adding proportions
5 processing ,+10% rice bran of respectively 90% stevia rebaudiana dregs, 70% STEVIA REBAUDIANA is arranged in solid medium adding proportion
+ 90% meter of+30% rice bran of slag ,+50% rice bran of 50% stevia rebaudiana dregs ,+70% rice bran of 30% stevia rebaudiana dregs and 10% stevia rebaudiana dregs
Chaff.Initial pH, water content, sterilization method, inoculum concentration and condition of culture are the same as 2.2.As shown in figure 3, stevia rebaudiana dregs addition is different
The rice bran of ratio has significant impact to the spore count measurer that trichoderma is fermented.The rice bran ferment effect of stevia rebaudiana dregs addition 50% is aobvious
It writes better than other processing, spore quantity reaches fresh weight 7 × 109cfu/g.The result shows that the rice bran of stevia rebaudiana dregs addition 50% is most
Ratio of greater inequality example.
2.4 shallow tray fermentation thickness
5 processing, respectively 1,2,3,4 and 5cm is arranged in shallow tray fermentation material thickness.Material be 50% stevia rebaudiana dregs+
50% rice bran.Initial pH, water content, sterilization method, inoculum concentration, temperature, humidity and incubation time are the same as 2.2.As shown in figure 4,
Shallow tray fermentation material thickness has significant impact to trichoderma fermentation spore count measurer.Within 1-3cm, trichoderma fermentation spore quantity
As the increase of material thickness is also in rising trend.However when material thickness be greater than 3cm after, trichoderma ferment spore quantity with
The increase of material thickness is on a declining curve, and the above results show that 3cm material thickness is optimal.
2.5 shallow tray fermentation water content
5 processing, respectively 50%, 55%, 60%, 65% and 70% is arranged in shallow tray fermentation water content.Material is 50%
+ 50% rice bran of stevia rebaudiana dregs.Initial pH, sterilization method, inoculum concentration, temperature, humidity, material thickness and incubation time are the same as 2.2.
As shown in figure 5, shallow tray fermentation material moisture has significant impact to trichoderma fermentation spore count measurer.When water content is lower than
60%, trichoderma ferments spore quantity as the increase of water content is in rising trend；After material moisture is higher than 60%, trichoderma
Spore quantity of fermenting is on a declining curve as water content increases, and the above results show that 60% water content is optimal.
2.6 shallow tray fermentation inoculum concentrations
5 processing, respectively 5%, 7.5%, 10%, 12.5% and 15% is arranged in shallow tray fermentation inoculum concentration.Material is
+ 50% rice bran of 50% stevia rebaudiana dregs.Initial pH, water content, sterilization method, temperature, humidity, material thickness and incubation time are same
2.2.As shown in fig. 6, shallow tray fermentation material inoculative proportion has significant impact to trichoderma fermentation spore count measurer.Work as inoculative proportion
Lower than 10%, trichoderma ferments spore quantity as the increase of inoculative proportion is in rising trend；After inoculative proportion is higher than 10%,
Trichoderma ferment spore quantity with the not no conspicuousness of inoculative proportion increase difference.In order to save industrial production cost, Wo Menyou
Selecting 10% is inoculative proportion.
2.7 shallow tray fermentation incubation times
Shallow tray fermentation inoculum concentration is arranged 5 and handles, and respectively 2,3,4,5 and 6 days.Material is 50% stevia rebaudiana dregs+50%
Rice bran.Initial pH, water content, sterilization method, inoculum concentration, temperature, humidity and material thickness are the same as 2.2.As shown in fig. 7, tray
Fermentation time has significant impact to trichoderma fermentation spore count measurer.Within 4 days, when trichoderma ferments spore quantity with inoculation
Between increase it is in rising trend；After 4 days, trichoderma ferments spore quantity as the time increases the difference of not conspicuousness.For
Saving time production cost, we preferably 4 days be fermentation time.
2.8 trichoderma strain potting growgh promoting effects
1 control and 5 processing is arranged in pot experiment, is do not apply fertilizer control treatment (CK) respectively；The processing of trichoderma strain
(T1)；Chemical fertilizer handles (T2)；Chemical fertilizer+trichoderma processing (T3)；Organic fertilizer handles (T4)；Organic fertilizer+trichoderma processing (T5), wherein
The trichoderma quantity that T1, T3 and T5 are added is consistent, and the npk nutrient that T2, T3, T4 and T5 are applied is consistent.By Fig. 8 and
Table 2 is it is found that compare control treatment, and only application trichoderma strain does not differ significantly corn items biomass indexes bacterium；It compares
Chemical fertilizer, chemical fertilizer+trichoderma strain do not differ significantly corn items biomass indexes bacterium yet；Compared to organic fertilizer, organic fertilizer+wood
Mould species have significant facilitation to the stem thickness of corn, ground fresh weight and ground dry weight.The above results show trichoderma strain
Cooperate organic fertilizer that there are good growgh promoting effects to crop, which has the use of trichoderma strain product of the present invention important
Directive significance.
Influence of 2 different disposal of table to corn crop biomass
Note: control treatment of not applying fertilizer (CK)；Trichoderma strain handles (T1)；Chemical fertilizer handles (T2)；Chemical fertilizer+trichoderma processing
(T3)；Organic fertilizer handles (T4)；Organic fertilizer+trichoderma strain processing (T5)
Claims (8)
1. a kind of method using stevia rebaudiana dregs solid fermentation preparation trichoderma strain, it is characterised in that by 10-15% trichoderma liquid
The shallow tray fermentation material after sterilizing is added in strain, is uniformly mixed, 26-28 DEG C of shallow tray fermentation temperature, humidity 70-90%, Wan Jijie
Clean room culture 4 days；The shallow tray fermentation material is using the mixture of stevia rebaudiana dregs and rice bran as raw material, by the way that acidolysis ammonia is added
Base acid adjusts material pH to 2.5-3.0, and tap water is adjusted obtained by water content to 60-65%.
2. according to the method described in claim 1, it is characterized in that stevia rebaudiana dregs are that water content is less than after drying and processing
10%, partial size is less than 5mm.
3. according to the method described in claim 1, it is characterized in that the shallow tray fermentation material be with 50% stevia rebaudiana dregs+
50% rice bran is raw material, adjusts material pH to 2.5-3.0 by acidolysis amino acid, tap water adjusts water content to 60% gained.
4. according to the method described in claim 3, it is characterized in that shallow tray fermentation material is using 115 DEG C of solid sterilization tank sterilizings 40
Minute, 10% trichoderma liquid spawn is seeded in sterilization tank material from strain liquid fermentor using culture transferring pipeline after cooling,
Strain and material are mixed using sterilization tank stirring system.
5. according to the method described in claim 1, it is characterized in that shallow tray fermentation material thickness be 2-4cm, preferably 2-3cm, into
The preferred 3cm of one step.
6. according to the method described in claim 1, it is characterized in that in acidolysis amino acid free aminoacid content be 15%, dissociate
Hydrogen ion concentration is 3M.
7. the trichoderma solid spawn prepared according to method of any of claims 1-7, it is characterised in that reesei spores
Quantity reaches dry weight 1.75 × 1010cfu/g。
8. trichoderma solid spawn cooperation organic fertilizer as claimed in claim 7 is applied in the application in corn crop growth-promoting.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN109370911A (en) * | 2018-10-18 | 2019-02-22 | 山东农业大学 | A method of Trichoderma atroviride biocontrol agent is prepared using stevia rebaudiana dregs |
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| CN110982711B (en) * | 2019-12-04 | 2020-08-07 | 南京农业大学 | Method for open production of trichoderma solid strain |
| CN112745151A (en) * | 2021-01-11 | 2021-05-04 | 南京农业大学 | Composite biological organic fertilizer containing bacillus and trichoderma and preparation method thereof |
| CN113528356A (en) * | 2021-08-17 | 2021-10-22 | 南京农业大学 | Solid trichoderma preparation fermented by tea leaves and application thereof in tea seedling culture |
| CN113528356B (en) * | 2021-08-17 | 2023-02-17 | 南京农业大学 | Solid trichoderma preparation fermented by tea leaves and application of solid trichoderma preparation in tea seedling raising |
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