WO2002031177A3 - Verfahren zur synthese von peptiden, peptidmimetika und proteinen - Google Patents

Verfahren zur synthese von peptiden, peptidmimetika und proteinen Download PDF

Info

Publication number
WO2002031177A3
WO2002031177A3 PCT/EP2001/011500 EP0111500W WO0231177A3 WO 2002031177 A3 WO2002031177 A3 WO 2002031177A3 EP 0111500 W EP0111500 W EP 0111500W WO 0231177 A3 WO0231177 A3 WO 0231177A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
enzyme
peptide mimetics
synthesising peptides
peptides
Prior art date
Application number
PCT/EP2001/011500
Other languages
English (en)
French (fr)
Other versions
WO2002031177A2 (de
Inventor
Frank Bordusa
Hans-Dieter Jakubke
Original Assignee
Roche Diagnostics Gmbh
Hoffmann La Roche
Frank Bordusa
Hans-Dieter Jakubke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh, Hoffmann La Roche, Frank Bordusa, Hans-Dieter Jakubke filed Critical Roche Diagnostics Gmbh
Priority to AU2001295592A priority Critical patent/AU2001295592A1/en
Priority to CA002423294A priority patent/CA2423294C/en
Priority to EP01976273A priority patent/EP1326995B8/de
Priority to US10/398,493 priority patent/US7459526B2/en
Priority to DE50111910T priority patent/DE50111910D1/de
Priority to JP2002534542A priority patent/JP3892397B2/ja
Publication of WO2002031177A2 publication Critical patent/WO2002031177A2/de
Publication of WO2002031177A3 publication Critical patent/WO2002031177A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21004—Trypsin (3.4.21.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Das Verfahren zur Herstellung von Peptiden, Peptidmimetika und Proteinen ist dadurch charakterisiert, dass man als Biokatalysator ein Enzym zusammen mit einem Substratmimetikum einsetzt. Die native Spezifität des Enzyms wird durch chemische oder gentechnische Manipulationen verändert, Substratmimetikum ist eine Carboxylkomponente mit einer Esterabgangsgruppe, deren Spezifitätsdeterminante der Erkennungsregion des Enzyms angepasst ist. Beansprucht wird ferner die Trypsinvariante D 189K,K60E und ihre Verwendung als CN-Ligase bei Segmentkondensationen.
PCT/EP2001/011500 2000-10-07 2001-10-05 Verfahren zur synthese von peptiden, peptidmimetika und proteinen WO2002031177A2 (de)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2001295592A AU2001295592A1 (en) 2000-10-07 2001-10-05 Method for synthesising peptides, peptide mimetics and proteins
CA002423294A CA2423294C (en) 2000-10-07 2001-10-05 Method for synthesising peptides, peptide mimetics and proteins
EP01976273A EP1326995B8 (de) 2000-10-07 2001-10-05 Verfahren zur synthese von peptiden, peptidmimetika und proteinen
US10/398,493 US7459526B2 (en) 2000-10-07 2001-10-05 Method for synthesizing peptides, peptide mimetics and proteins
DE50111910T DE50111910D1 (de) 2000-10-07 2001-10-05 Verfahren zur synthese von peptiden, peptidmimetika und proteinen
JP2002534542A JP3892397B2 (ja) 2000-10-07 2001-10-05 ペプチド、ペプチド模倣物およびタンパク質を合成するための方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10049673.3 2000-10-07
DE10049673A DE10049673A1 (de) 2000-10-07 2000-10-07 Verfahren zur Herstellung von Peptiden, Peptidmimetika und Proteinen

Publications (2)

Publication Number Publication Date
WO2002031177A2 WO2002031177A2 (de) 2002-04-18
WO2002031177A3 true WO2002031177A3 (de) 2003-03-13

Family

ID=7658978

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/011500 WO2002031177A2 (de) 2000-10-07 2001-10-05 Verfahren zur synthese von peptiden, peptidmimetika und proteinen

Country Status (9)

Country Link
US (1) US7459526B2 (de)
EP (1) EP1326995B8 (de)
JP (1) JP3892397B2 (de)
AT (1) ATE351917T1 (de)
AU (1) AU2001295592A1 (de)
CA (1) CA2423294C (de)
DE (2) DE10049673A1 (de)
ES (1) ES2278787T3 (de)
WO (1) WO2002031177A2 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7335504B2 (en) 2003-06-18 2008-02-26 Direvo Biotechnology Ag Engineered enzymes and uses thereof
JP4538501B2 (ja) * 2004-08-13 2010-09-08 エフ．ホフマン−ラ ロシュ アーゲー ポリペプチドのｃ末端修飾
RU2584572C2 (ru) 2010-02-11 2016-05-20 Ф.Хоффманн-Ля Рош Аг Способ получения полипептида, конъюгированного с поли(этиленгликолевым) фрагментом, нуклеиновая кислота и слитый полипептид, предназначенные для применения в способе
CN105296452B (zh) * 2015-10-15 2018-10-26 天津大学 一种突变型Sus scrofa猪源胰蛋白酶及其编码基因及获取方法和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002615A1 (en) * 1990-08-09 1992-02-20 Genentech, Inc. Serine protease variants having peptide ligase activity
WO1994018329A2 (en) * 1993-02-04 1994-08-18 Genentech, Inc. Serine protease variants having peptide ligase activity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992002615A1 (en) * 1990-08-09 1992-02-20 Genentech, Inc. Serine protease variants having peptide ligase activity
WO1994018329A2 (en) * 1993-02-04 1994-08-18 Genentech, Inc. Serine protease variants having peptide ligase activity

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BORDUSA F: "Nonconventional amide bond formation catalysis: programming enzyme specificity with substrate mimetics.", BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH = REVISTA BRASILEIRA DE PESQUISAS MEDICAS E BIOLOGICAS / SOCIEDADE BRASILEIRA DE BIOFISICA... [ET AL.]. BRAZIL MAY 2000, vol. 33, no. 5, May 2000 (2000-05-01), pages 469 - 485, XP008009710, ISSN: 0100-879X *
CEROVSKY V ET AL: "PROTEASE-CATALYZED FRAGMENT CONDENSATION VIA SUBSTRATE MIMETIC STRATEGY: A USEFUL COMBINATION OF SOLID-PHASE PEPTIDE SYNTHESIS WITH ENZYMATIC METHODS", JOURNAL OF PEPTIDE RESEARCH, MUNKSGAARD INTERNATIONAL PUBLISHERS, COPENHAGEN, DK, vol. 55, no. 4, April 2000 (2000-04-01), pages 325 - 329, XP000930485, ISSN: 1397-002X *
CEROVSKY V ET AL: "Semisynthesis of Ht31(493--515): involvement of PKA-anchoring proteins in the regulation of the cAMP-dependent chloride current in heart cells.", CHEMBIOCHEM: A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY. GERMANY 18 AUG 2000, vol. 1, no. 2, 18 August 2000 (2000-08-18), pages 126 - 129, XP002218038, ISSN: 1439-4227 *
GRUNBERG R ET AL: "Peptide bond formation mediated by substrate mimetics. Structure-guidedoptimization of trypsin for synthesis.", EUROPEAN JOURNAL OF BIOCHEMISTRY, (2000 DEC) 267 (24) 7024-30. JOURNAL CODE: 0107600., XP002218037 *
JAKUBKE H D (REPRINT): "PEPTIDE LIGASES - TOOLS FOR PEPTIDE-SYNTHESIS", ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, (03 FEB 1995) VOL. 34, NO. 2, PP. 175-177., XP002218039 *
KURTH T ET AL: "Engineering the S1' subsite of trypsin: design of a protease which cleaves between dibasic residues.", BIOCHEMISTRY, (1998 AUG 18) 37 (33) 11434-40. JOURNAL CODE: 0370623., XP002218040 *
XU S ET AL: "Enzymatic coupling of specific peptides at nonspecific ligation sites: effect of Asp189Glu mutation in trypsin on substrate mimetic-mediated reactions.", JOURNAL OF ORGANIC CHEMISTRY, (2001 MAR 9) 66 (5) 1627-32. JOURNAL CODE: 2985193R., 25 January 2001 (2001-01-25), XP002218036 *

Also Published As

Publication number Publication date
JP3892397B2 (ja) 2007-03-14
DE10049673A1 (de) 2002-04-25
JP2004511234A (ja) 2004-04-15
US7459526B2 (en) 2008-12-02
US20040034197A1 (en) 2004-02-19
CA2423294A1 (en) 2002-04-18
EP1326995B1 (de) 2007-01-17
AU2001295592A1 (en) 2002-04-22
CA2423294C (en) 2008-02-19
EP1326995A2 (de) 2003-07-16
DE50111910D1 (de) 2007-03-08
ATE351917T1 (de) 2007-02-15
WO2002031177A2 (de) 2002-04-18
EP1326995B8 (de) 2007-02-28
ES2278787T3 (es) 2007-08-16

Similar Documents

Publication Publication Date Title
RU2225220C1 (ru) Слитый белок, обладающий in vivo повышенной активностью эритропоэтина
WO2002079232A3 (en) Reducing the immunogenicity of fusion proteins
WO2001029242A3 (en) Post-translational modification of recombinant proteins produced in plants
AU1551701A (en) Novel carbonyl reductase, gene thereof and method of using the same
WO2003066842A3 (de) Verfahren zur herstellung von rekombinanten proteinen in mikroorganismen
WO2002018443A3 (en) A recombinant monoclonal antibody to phosphotyrosine-containing proteins
WO2002061064A3 (de) Verfahren zur herstellung von rekombinantem trypsin
WO2004035618A3 (en) Nucleic acids coding for adhesion factor of group b streptococcus, adhesion factors of group b streptococcus and further uses thereof
DE69926627D1 (de) Insulinartigen wachstumsfaktor bindendes protein -3 variante
WO2002031177A3 (de) Verfahren zur synthese von peptiden, peptidmimetika und proteinen
ATE317443T1 (de) N-terminal verlängerte proteine exprimiert in hefe
Kim et al. A processing enzyme for prorenin in mouse submandibular gland. Purification and characterization.
WO2002103026A3 (en) Method for the recombinant production of peptidic antiviral fusion inhibitors, and acetylation of gb41 fragments
ATE466876T1 (de) Immunogene konstrukte
WO2001034811A3 (en) Bacillus thuringiensis hybrid toxins
WO2003054155A3 (en) Pichia pastoris formate dehydrogenase and uses therefor
WO2002048322A3 (de) Rekombinante schweineleberesterasen, deren verwendung sowie ein verfahren zu deren herstellung
WO2002046379A3 (en) Glucuronyl c5-epimerase, dna encoding the same and uses thereof
WO2000055191A3 (en) Recombinant haemophilus influenzae adhesin proteins
WO2004053052A3 (en) HUMAN RECOMBINANT ENDOOLIGOPEPTIDASE A (hEOPA)
EP1721976A4 (de) Modifizierter promotor
WO2000065060A3 (de) Dna-sequenz und rekombinante herstellung eines graminaen-allergens
WO2004089973A3 (en) Peptides, polypeptides, and proteins of reduced immunogenicity and methods for their production
WO2008029138A3 (en) Peptides and methods
WO1999055876A3 (de) Polypeptid mit immunogenen eigenschaften und veränderten biologischen funktionen eines proteins

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2001976273

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2423294

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 10398493

Country of ref document: US

Ref document number: 2002534542

Country of ref document: JP

WWP Wipo information: published in national office

Ref document number: 2001976273

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWG Wipo information: grant in national office

Ref document number: 2001976273

Country of ref document: EP