WO2002006778A1 - Systeme et procede pour microscopie de diffusion raman anti-stokes coherente a retrodetection - Google Patents

Systeme et procede pour microscopie de diffusion raman anti-stokes coherente a retrodetection Download PDF

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Publication number
WO2002006778A1
WO2002006778A1 PCT/US2001/019678 US0119678W WO0206778A1 WO 2002006778 A1 WO2002006778 A1 WO 2002006778A1 US 0119678 W US0119678 W US 0119678W WO 0206778 A1 WO0206778 A1 WO 0206778A1
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WO
WIPO (PCT)
Prior art keywords
stokes
field
pump
epi
sample
Prior art date
Application number
PCT/US2001/019678
Other languages
English (en)
Inventor
Xiaoliang Sunney Xie
Andreas Volkmer
Ji-Xin Cheng
Original Assignee
President And Fellows Of Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by President And Fellows Of Harvard College filed Critical President And Fellows Of Harvard College
Priority to AT01957072T priority Critical patent/ATE543080T1/de
Priority to EP01957072A priority patent/EP1311813B9/fr
Publication of WO2002006778A1 publication Critical patent/WO2002006778A1/fr
Priority to US10/345,677 priority patent/US6809814B2/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01N15/1433—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N2021/653—Coherent methods [CARS]

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  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne des systèmes et des procédés permettant de détecter un signal de diffusion Raman anti-Sokes cohérente (DRASC) à partir d'un échantillon microscopique dans une rétrodirection. Dans un mode de réalisation, ce système comprend au moins deux sources possédant un source pompe pour générer un champ pompe à une fréquence pompe, une source Stoke pour générer un champ Stoke à une fréquence Stoke différente de la fréquence pompe, des dispositifs optiques (64) pour diriger les faisceaux (60) de pompe et de Stoke d'une manière colinéaire à travers une lentille de focalisation (66) vers un point focal commun dans un échantillon (70), et un dispositif optiques de détection qui image les DRASC dans une rétrodirection, laquelle est générée par l'interaction des champs (60) pompe et Stoke avec l'échantillon (70) et est recueillie par la même lentille de focalisation (66), en direction d'un rétrodétecteur (78).
PCT/US2001/019678 2000-07-13 2001-06-20 Systeme et procede pour microscopie de diffusion raman anti-stokes coherente a retrodetection WO2002006778A1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AT01957072T ATE543080T1 (de) 2000-07-13 2001-06-20 System und verfahren für die epidetektions- kohärenz-anti-stokes-raman-streumikroskopie
EP01957072A EP1311813B9 (fr) 2000-07-13 2001-06-20 Systeme et procede pour microscopie de diffusion raman anti-stokes coherente a retrodetection
US10/345,677 US6809814B2 (en) 2000-07-13 2003-01-16 System and method for epi-detected coherent anti-stokes raman scattering microscopy

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21809100P 2000-07-13 2000-07-13
US60/218,091 2000-07-13

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/345,677 Continuation US6809814B2 (en) 2000-07-13 2003-01-16 System and method for epi-detected coherent anti-stokes raman scattering microscopy

Publications (1)

Publication Number Publication Date
WO2002006778A1 true WO2002006778A1 (fr) 2002-01-24

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/019678 WO2002006778A1 (fr) 2000-07-13 2001-06-20 Systeme et procede pour microscopie de diffusion raman anti-stokes coherente a retrodetection

Country Status (4)

Country Link
US (1) US6809814B2 (fr)
EP (1) EP1311813B9 (fr)
AT (1) ATE543080T1 (fr)
WO (1) WO2002006778A1 (fr)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7256885B2 (en) 2003-01-29 2007-08-14 Yeda Research And Development Company Ltd. Coherently controlled nonlinear Raman spectroscopy and microscopy
US7436501B2 (en) 2004-07-06 2008-10-14 Olympus Corporation Microscope
US7826051B2 (en) 2004-05-27 2010-11-02 Yeda Research And Development Company Ltd. Coherently controlled nonlinear raman spectroscopy
CN106990089A (zh) * 2017-04-01 2017-07-28 上海理工大学 同步降频的相干反斯托克斯拉曼散射成像系统及成像方法
US11796449B2 (en) 2013-10-30 2023-10-24 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
US11889830B2 (en) 2019-04-18 2024-02-06 Abs Global, Inc. System and process for continuous addition of cryoprotectant

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005029149A1 (fr) * 2003-09-25 2005-03-31 Leica Microsystems Cms Gmbh Microscope a eclairage evanescent
US7388668B2 (en) * 2004-06-09 2008-06-17 President & Fellows Of Harvard College Phase sensitive heterodyne coherent anti-Stokes Raman scattering micro-spectroscopy and microscopy
JP2008529062A (ja) 2005-01-21 2008-07-31 プレジデント アンド フェローズ オブ ハーバード カレッジ オフィス オブ テクノロジー アンド トレードマーク ライセンシング 非線形振動分光法および顕微鏡法のための２周波出力を提供する同調可能な光パラメトリック発振器レーザシステムを提供するシステムおよび方法
US7414729B2 (en) 2005-10-13 2008-08-19 President And Fellows Of Harvard College System and method for coherent anti-Stokes Raman scattering endoscopy
US7352458B2 (en) * 2005-10-26 2008-04-01 President And Fellows Of Harvard College System and method for high sensitivity vibrational imaging with frequency modulation coherent anti-stokes Raman scattering analyses
EP2076172A4 (fr) * 2006-10-27 2012-03-21 Aretais Inc Utilisation de techniques de raman cohérentes dans des buts de diagnostic médical et thérapeutiques, et leurs techniques de calibrage
CN101617407A (zh) * 2006-10-27 2009-12-30 阿里戴斯公司 使用量子系统识别和量子控制技术用于医疗诊断和治疗目的
US7480045B2 (en) * 2006-10-31 2009-01-20 Academia Sinica Controlling pulses in optical microscopy
DE102006056429B3 (de) 2006-11-28 2008-02-14 Leica Microsystems Cms Gmbh Lasermikroskop mit räumlich trennendem Strahlteiler
US7869051B2 (en) * 2007-01-19 2011-01-11 Stc. Unm System and method for ratiometric non-linear coherent imaging
DE102007021378A1 (de) * 2007-05-04 2008-11-06 Ape Angewandte Physik Und Elektronik Gmbh Verfahren und optische Anordnung zum Erzeugen eines nicht-linearen optischen Signals an einem durch ein Anregungsfeld angeregten Material sowie Verwendung des Verfahrens und der optischen Anordnung
KR100860947B1 (ko) * 2007-06-08 2008-09-30 한국표준과학연구원 적외선 비선형 분자진동 분광 이미징 장치
WO2009149733A1 (fr) * 2008-06-13 2009-12-17 Embl Heidelberg Cytomètre en flux trieur de future génération
US8027032B2 (en) * 2008-08-22 2011-09-27 President & Fellows Of Harvard College Microscopy imaging system and method employing stimulated raman spectroscopy as a contrast mechanism
US8120772B2 (en) * 2008-11-03 2012-02-21 The United States of America as represented by the Secretary of Commerce, the National Institute of Standards and Technology Method for NRB-free nonlinear vibrational spectroscopy and miscroscopy
US8994932B2 (en) 2008-12-20 2015-03-31 Purdue Research Foundation Multimodal platform for nonlinear optical microscopy and microspectroscopy
US9285575B2 (en) 2009-01-26 2016-03-15 President And Fellows Of Harvard College Systems and methods for selective detection and imaging in coherent Raman microscopy by spectral excitation shaping
US8264694B2 (en) * 2009-03-16 2012-09-11 Ut-Battelle, Llc Quantitative phase-contrast and excitation-emission systems
US8248614B2 (en) * 2009-03-16 2012-08-21 Ut-Battelle, Llc Quantitative phase-imaging systems
WO2011152834A1 (fr) * 2009-06-05 2011-12-08 The Penn State Research Foundation Holographie de raman anti-stokes cohérente
FR2955663B1 (fr) 2010-01-22 2014-09-12 Ct Nat De La Rech C N R S Methode pour la detection d'un signal optique non lineaire resonant et dispositif pour la mise en oeuvre de ladite methode
WO2011162787A1 (fr) 2010-06-22 2011-12-29 President And Fellows Of Harvard College Systèmes et procédés réalisant une détection efficace de l'éclairage rétrodiffusé en microscopie à transfert de modulation ou en micro-spectroscopie
GB201013321D0 (en) 2010-08-06 2010-09-22 King S College London Method and apparatus for non-resonant background reduction in Coherent anti-strokes Raman Scattering (CARS) spectroscopy
US20130267855A1 (en) * 2011-10-28 2013-10-10 Kazuo Tsubota Comprehensive measuring method of biological materials and treatment method using broadly tunable laser
US9634454B1 (en) 2012-01-17 2017-04-25 President And Fellows Of Harvard College Laser illumination systems and methods for dual-excitation wavelength non-linear optical microscopy and micro-spectroscopy systems
US8792156B1 (en) 2012-01-17 2014-07-29 President And Fellows Of Harvard College Laser illumination systems and methods for dual-excitation wavelength non-linear optical microscopy and micro-spectroscopy systems
FR3003949B1 (fr) * 2013-03-26 2015-05-01 Univ Aix Marseille Dispositif et methode de detection raman stimulee.
US9506858B2 (en) * 2013-05-09 2016-11-29 The Board Of Trustees Of The University Of Illinois Optical parametric amplification of weak signals for imaging biological tissue
US20160178439A1 (en) * 2013-06-17 2016-06-23 Invenio Imaging Inc. Methods and systems for coherent raman scattering
US9778193B2 (en) 2013-08-22 2017-10-03 Kinetic River Corp. Methods and apparatuses for label-free particle analysis
US10743848B2 (en) 2015-09-25 2020-08-18 The Regents Of The University Of Michigan Biopsy device for coherent Raman imaging
CN109765213B (zh) * 2019-03-27 2024-03-29 苏州威邦震电光电技术有限公司 相干反斯托克斯拉曼散射显微镜成像装置

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4284354A (en) * 1979-10-24 1981-08-18 Bell Telephone Laboratories, Incorporated Sensitive nonlinear optical spectroscopy
US4405237A (en) * 1981-02-04 1983-09-20 The United States Of America As Represented By The Secretary Of The Navy Coherent anti-Stokes Raman device
US5617206A (en) * 1995-12-04 1997-04-01 Phi, Applied Physical Sciences International Compact laser diode monitor using defined laser momentum vectors to cause emission of a coherent photon in a selected direction
US6108081A (en) * 1998-07-20 2000-08-22 Battelle Memorial Institute Nonlinear vibrational microscopy

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0342553A (ja) 1989-07-11 1991-02-22 Tosoh Corp 顕微ラマン測定装置
US5286970A (en) 1990-11-19 1994-02-15 At&T Bell Laboratories Near field optical microscopic examination of a biological specimen
US5674698A (en) 1992-09-14 1997-10-07 Sri International Up-converting reporters for biological and other assays using laser excitation techniques
US5418797A (en) 1993-01-15 1995-05-23 The United States Of America As Represented By The Secretary Of The Navy Time gated imaging through scattering material using polarization and stimulated raman amplification
JPH10512959A (ja) 1995-09-19 1998-12-08 コーネル・リサーチ・ファンデーション・インコーポレイテッド 多光子レーザ顕微鏡法
US5719399A (en) 1995-12-18 1998-02-17 The Research Foundation Of City College Of New York Imaging and characterization of tissue based upon the preservation of polarized light transmitted therethrough
US6151522A (en) 1998-03-16 2000-11-21 The Research Foundation Of Cuny Method and system for examining biological materials using low power CW excitation raman spectroscopy

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4284354A (en) * 1979-10-24 1981-08-18 Bell Telephone Laboratories, Incorporated Sensitive nonlinear optical spectroscopy
US4405237A (en) * 1981-02-04 1983-09-20 The United States Of America As Represented By The Secretary Of The Navy Coherent anti-Stokes Raman device
US5617206A (en) * 1995-12-04 1997-04-01 Phi, Applied Physical Sciences International Compact laser diode monitor using defined laser momentum vectors to cause emission of a coherent photon in a selected direction
US6108081A (en) * 1998-07-20 2000-08-22 Battelle Memorial Institute Nonlinear vibrational microscopy

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7256885B2 (en) 2003-01-29 2007-08-14 Yeda Research And Development Company Ltd. Coherently controlled nonlinear Raman spectroscopy and microscopy
US7403282B2 (en) 2003-01-29 2008-07-22 Yeda Research And Development Company Ltd. Coherently controlled nonlinear Raman spectroscopy and microscopy
US7826051B2 (en) 2004-05-27 2010-11-02 Yeda Research And Development Company Ltd. Coherently controlled nonlinear raman spectroscopy
US7436501B2 (en) 2004-07-06 2008-10-14 Olympus Corporation Microscope
US11796449B2 (en) 2013-10-30 2023-10-24 Abs Global, Inc. Microfluidic system and method with focused energy apparatus
CN106990089A (zh) * 2017-04-01 2017-07-28 上海理工大学 同步降频的相干反斯托克斯拉曼散射成像系统及成像方法
CN106990089B (zh) * 2017-04-01 2019-06-04 上海理工大学 同步降频的相干反斯托克斯拉曼散射成像系统及成像方法
US11889830B2 (en) 2019-04-18 2024-02-06 Abs Global, Inc. System and process for continuous addition of cryoprotectant

Also Published As

Publication number Publication date
EP1311813B9 (fr) 2012-05-02
EP1311813A4 (fr) 2006-05-10
US20030160955A1 (en) 2003-08-28
EP1311813B1 (fr) 2012-01-25
ATE543080T1 (de) 2012-02-15
EP1311813A1 (fr) 2003-05-21
US6809814B2 (en) 2004-10-26

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