WO2001049830A3 - Enzymes lysosomales ameliorees et activateurs d"enzymes lysosomales - Google Patents

Enzymes lysosomales ameliorees et activateurs d"enzymes lysosomales Download PDF

Info

Publication number
WO2001049830A3
WO2001049830A3 PCT/DK2000/000743 DK0000743W WO0149830A3 WO 2001049830 A3 WO2001049830 A3 WO 2001049830A3 DK 0000743 W DK0000743 W DK 0000743W WO 0149830 A3 WO0149830 A3 WO 0149830A3
Authority
WO
WIPO (PCT)
Prior art keywords
lysosomal
improved
enzymes
enzyme activators
enzyme
Prior art date
Application number
PCT/DK2000/000743
Other languages
English (en)
Other versions
WO2001049830A2 (fr
WO2001049830A9 (fr
Inventor
Jens Sigurd Okkels
Anne Dam Jensen
Torben Halkier
Rikke Bolding Jensen
Hans Thalsgaard Schambye
Original Assignee
Maxygen Aps
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Aps filed Critical Maxygen Aps
Priority to EP00987208A priority Critical patent/EP1246915A2/fr
Priority to AU23522/01A priority patent/AU2352201A/en
Priority to IL15031400A priority patent/IL150314A0/xx
Priority to CA002412882A priority patent/CA2412882A1/fr
Priority to AU2001267337A priority patent/AU2001267337A1/en
Priority to US09/896,896 priority patent/US20030036181A1/en
Priority to EP01944987A priority patent/EP1299535A2/fr
Priority to JP2002507849A priority patent/JP2004504016A/ja
Priority to PCT/DK2001/000459 priority patent/WO2002002597A2/fr
Publication of WO2001049830A2 publication Critical patent/WO2001049830A2/fr
Publication of WO2001049830A3 publication Critical patent/WO2001049830A3/fr
Publication of WO2001049830A9 publication Critical patent/WO2001049830A9/fr
Priority to US10/967,088 priority patent/US20060160177A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01045—Glucosylceramidase (3.2.1.45), i.e. beta-glucocerebrosidase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2465—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on alpha-galactose-glycoside bonds, e.g. alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01018—Exo-alpha-sialidase (3.2.1.18), i.e. trans-sialidase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01022—Alpha-galactosidase (3.2.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01046—Galactosylceramidase (3.2.1.46)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01076—L-Iduronidase (3.2.1.76)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Reproductive Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Cette invention concerne un polypeptide pris dans le groupe des enzymes lysosomales et des activateurs d"enzymes lysosomales, qui, par comparaison à une enzyme ou un activateur parent correspondant, renferment au moins un site introduit de glycosylation. Grâce à l"introduction de sites de glycosylation supplémentaires, l"enzyme lysosomale glycosilée ou l"activateur présente une activité améliorée in vivo et ont une action thérapeutique plus efficace contre les maladies lysosomales.
PCT/DK2000/000743 1999-12-30 2000-12-29 Enzymes lysosomales ameliorees et activateurs d"enzymes lysosomales WO2001049830A2 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
EP00987208A EP1246915A2 (fr) 1999-12-30 2000-12-29 Enzymes lysosomales ameliorees et activateurs d'enzymes lysosomales
AU23522/01A AU2352201A (en) 1999-12-30 2000-12-29 Improved lysosomal enzymes and lysosomal enzyme activators
IL15031400A IL150314A0 (en) 1999-12-30 2000-12-29 Improved lysosomal enzymes and lysosomal enzyme activators
US09/896,896 US20030036181A1 (en) 2000-06-30 2001-06-29 Peptide extended glycosylated polypeptides
AU2001267337A AU2001267337A1 (en) 2000-06-30 2001-06-29 Peptide extended glycosylated polypeptides
CA002412882A CA2412882A1 (fr) 2000-06-30 2001-06-29 Polypeptides glycosyles a extension peptidique
EP01944987A EP1299535A2 (fr) 2000-06-30 2001-06-29 Polypeptides glycosyles a extension peptidique
JP2002507849A JP2004504016A (ja) 2000-06-30 2001-06-29 ペプチド拡張されたグリコシル化ポリペプチド
PCT/DK2001/000459 WO2002002597A2 (fr) 2000-06-30 2001-06-29 Polypeptides glycosyles a extension peptidique
US10/967,088 US20060160177A1 (en) 2000-06-30 2004-10-15 Peptide extended glycosylated polypeptides

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
DKPA199901891 1999-12-30
DKPA199901891 1999-12-30
DKPA200000865 2000-06-02
DKPA200000866 2000-06-02
DKPA200000865 2000-06-02
DKPA200000866 2000-06-02
DKPA200001027 2000-06-30
DKPA200001027 2000-06-30

Publications (3)

Publication Number Publication Date
WO2001049830A2 WO2001049830A2 (fr) 2001-07-12
WO2001049830A3 true WO2001049830A3 (fr) 2002-02-07
WO2001049830A9 WO2001049830A9 (fr) 2002-11-07

Family

ID=27439513

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2000/000743 WO2001049830A2 (fr) 1999-12-30 2000-12-29 Enzymes lysosomales ameliorees et activateurs d"enzymes lysosomales

Country Status (4)

Country Link
EP (1) EP1246915A2 (fr)
AU (1) AU2352201A (fr)
IL (1) IL150314A0 (fr)
WO (1) WO2001049830A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9220737B2 (en) 2003-04-27 2015-12-29 Protalix Ltd. Plant cell culture expressing human lysosomal proteins and uses thereof

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6639050B1 (en) 1997-07-21 2003-10-28 Ohio University Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
US7378506B2 (en) 1997-07-21 2008-05-27 Ohio University Synthetic genes for plant gums and other hydroxyproline-rich glycoproteins
WO2001077307A2 (fr) * 2000-04-06 2001-10-18 Exegenics, Inc. Systeme d'expression pour la production efficace d'enzymes lysosomales cliniquement efficaces (glucocerebrosidase)
US7138262B1 (en) 2000-08-18 2006-11-21 Shire Human Genetic Therapies, Inc. High mannose proteins and methods of making high mannose proteins
US8008252B2 (en) 2001-10-10 2011-08-30 Novo Nordisk A/S Factor VII: remodeling and glycoconjugation of Factor VII
US7226903B2 (en) 2001-10-10 2007-06-05 Neose Technologies, Inc. Interferon beta: remodeling and glycoconjugation of interferon beta
US7157277B2 (en) 2001-11-28 2007-01-02 Neose Technologies, Inc. Factor VIII remodeling and glycoconjugation of Factor VIII
US7297511B2 (en) 2001-10-10 2007-11-20 Neose Technologies, Inc. Interferon alpha: remodeling and glycoconjugation of interferon alpha
US7125843B2 (en) 2001-10-19 2006-10-24 Neose Technologies, Inc. Glycoconjugates including more than one peptide
US7265085B2 (en) 2001-10-10 2007-09-04 Neose Technologies, Inc. Glycoconjugation methods and proteins/peptides produced by the methods
US7696163B2 (en) 2001-10-10 2010-04-13 Novo Nordisk A/S Erythropoietin: remodeling and glycoconjugation of erythropoietin
US7265084B2 (en) 2001-10-10 2007-09-04 Neose Technologies, Inc. Glycopegylation methods and proteins/peptides produced by the methods
US7795210B2 (en) 2001-10-10 2010-09-14 Novo Nordisk A/S Protein remodeling methods and proteins/peptides produced by the methods
US7439043B2 (en) 2001-10-10 2008-10-21 Neose Technologies, Inc. Galactosyl nucleotide sugars
WO2003031464A2 (fr) 2001-10-10 2003-04-17 Neose Technologies, Inc. Remodelage et glycoconjugaison de peptides
US7399613B2 (en) 2001-10-10 2008-07-15 Neose Technologies, Inc. Sialic acid nucleotide sugars
US7214660B2 (en) 2001-10-10 2007-05-08 Neose Technologies, Inc. Erythropoietin: remodeling and glycoconjugation of erythropoietin
US7179617B2 (en) 2001-10-10 2007-02-20 Neose Technologies, Inc. Factor IX: remolding and glycoconjugation of Factor IX
US7173003B2 (en) 2001-10-10 2007-02-06 Neose Technologies, Inc. Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF
US7473680B2 (en) 2001-11-28 2009-01-06 Neose Technologies, Inc. Remodeling and glycoconjugation of peptides
US7553930B2 (en) 2003-01-06 2009-06-30 Xencor, Inc. BAFF variants and methods thereof
KR20060003862A (ko) 2003-03-14 2006-01-11 네오스 테크놀로지스, 인크. 수용성분기폴리머 및 그 접합체
US8791070B2 (en) 2003-04-09 2014-07-29 Novo Nordisk A/S Glycopegylated factor IX
US7691603B2 (en) 2003-04-09 2010-04-06 Novo Nordisk A/S Intracellular formation of peptide conjugates
SG155777A1 (en) 2003-04-09 2009-10-29 Neose Technologies Inc Glycopegylation methods and proteins/peptides produced by the methods
MXPA05011832A (es) 2003-05-09 2006-02-17 Neose Technologies Inc Composiciones y metodos para la preparacion de mutantes de glicosilacion de la hormona de crecimiento humano.
US9005625B2 (en) 2003-07-25 2015-04-14 Novo Nordisk A/S Antibody toxin conjugates
IL157772A (en) 2003-09-04 2016-06-30 Bmr Solutions Ltd Compounds containing oligopeptide derived from turtle larvae and their use to encourage mammalian hemophysis
US20080305992A1 (en) 2003-11-24 2008-12-11 Neose Technologies, Inc. Glycopegylated erythropoietin
EP1694347B1 (fr) 2003-11-24 2013-11-20 BioGeneriX AG Erythropoiétine glycopegylée
US7842661B2 (en) 2003-11-24 2010-11-30 Novo Nordisk A/S Glycopegylated erythropoietin formulations
US8633157B2 (en) 2003-11-24 2014-01-21 Novo Nordisk A/S Glycopegylated erythropoietin
US7956032B2 (en) 2003-12-03 2011-06-07 Novo Nordisk A/S Glycopegylated granulocyte colony stimulating factor
US20060040856A1 (en) 2003-12-03 2006-02-23 Neose Technologies, Inc. Glycopegylated factor IX
US8361961B2 (en) 2004-01-08 2013-01-29 Biogenerix Ag O-linked glycosylation of peptides
AU2005206885B2 (en) 2004-01-14 2012-02-02 Ohio University Methods of producing peptides/proteins in plants and peptides/proteins produced thereby
WO2005110015A2 (fr) 2004-04-19 2005-11-24 Ohio University Glycoproteines reticulables et leurs methodes de fabrication
ATE455861T1 (de) 2004-05-04 2010-02-15 Novo Nordisk Healthcare Ag O-verknüpfte glykoformen von faktor vii und verfahren zu deren herstellung
US20080300173A1 (en) 2004-07-13 2008-12-04 Defrees Shawn Branched Peg Remodeling and Glycosylation of Glucagon-Like Peptides-1 [Glp-1]
WO2006031811A2 (fr) 2004-09-10 2006-03-23 Neose Technologies, Inc. Interferon alpha glycopegyle
ES2572779T3 (es) 2004-10-29 2016-06-02 Ratiopharm Gmbh Remodelación y glucopegilación del Factor de Crecimiento de Fibroblastos (FGF)
WO2006074467A2 (fr) 2005-01-10 2006-07-13 Neose Technologies, Inc. Facteur de stimulation de colonie de granulocytes glycopegylatees
EP1871795A4 (fr) 2005-04-08 2010-03-31 Biogenerix Ag Compositions et méthodes utilisées pour la préparation de mutants par glycosylation de l'hormone de croissance humaine résistant à la protéase
JP5216580B2 (ja) 2005-05-25 2013-06-19 ノヴォ ノルディスク アー／エス グリコペグ化第ｉｘ因子
US20070105755A1 (en) 2005-10-26 2007-05-10 Neose Technologies, Inc. One pot desialylation and glycopegylation of therapeutic peptides
US20090048440A1 (en) 2005-11-03 2009-02-19 Neose Technologies, Inc. Nucleotide Sugar Purification Using Membranes
ES2391657T3 (es) 2006-02-07 2012-11-28 Shire Human Genetic Therapies, Inc. Composiciones estabilizadas de proteínas que tienen un resto tiol libre
EP2029740B1 (fr) * 2006-05-31 2012-06-20 Genzyme Corporation Utilisation de polysaccharides pour faciliter une activité enzymatique
US8969532B2 (en) 2006-10-03 2015-03-03 Novo Nordisk A/S Methods for the purification of polypeptide conjugates comprising polyalkylene oxide using hydrophobic interaction chromatography
CA2887752C (fr) 2007-04-03 2020-03-24 Nico Luc Marc Callewaert Glycosylation de molecules
ES2406267T3 (es) 2007-04-03 2013-06-06 Biogenerix Ag Métodos de tratamiento usando G-CSF glicopegilado
BRPI0810343B1 (pt) 2007-05-07 2021-03-30 Protalix Ltd Dispositivo descartável para o cultivo e colheita de tecidos vegetais e/ou células, método de cultivo e colheita de um tecido vegetal e/ou células vegetais em um volume maior do que 400 litros e sistema de cultivo da célula vegetal
JP5876649B2 (ja) 2007-06-12 2016-03-02 ラツィオファルム ゲーエムベーハーｒａｔｉｏｐｈａｒｍ ＧｍｂＨ ヌクレオチド糖の改良製造法
US8207112B2 (en) 2007-08-29 2012-06-26 Biogenerix Ag Liquid formulation of G-CSF conjugate
US20130189239A1 (en) 2008-02-27 2013-07-25 Novo Nordisk A/S Conjugated Factor VIII Molecules
CN105126086B (zh) 2009-07-28 2019-05-14 夏尔人类遗传性治疗公司 用于治疗戈谢病的组合物和方法
CA2775938C (fr) 2009-09-29 2021-08-10 Oxyrane Uk Limited Hydrolyse de la liaison du mannose-1-phospho-6-mannose au phospho-6-mannose
JP2013511272A (ja) 2009-11-19 2013-04-04 オキシレイン ユーケー リミテッド 哺乳類様複合ｎ−グリカンを生成する酵母株
WO2012012461A2 (fr) * 2010-07-19 2012-01-26 Shire Human Genetic Therapies, Inc. Lignée cellulaire optimisée pour le codon de récepteur de mannose de type c 1 (mrc1) et ses utilisations
CN103261432A (zh) 2010-09-29 2013-08-21 奥克西雷恩英国有限公司 磷酸化的n-聚糖的脱甘露糖基化
KR101983572B1 (ko) 2010-09-29 2019-05-29 옥시레인 유케이 리미티드 만노스-1-포스포-6-만노스 결합의 캡핑제거 및 인산화 n-글리칸의 탈만노실이 가능한 만노시다제 및 당단백질의 활용을 통한 포유류 세포의 촉진방법
US8962564B2 (en) 2010-11-08 2015-02-24 Amicus Therapeutics, Inc. Variant, recombinant beta-glucocerebrosidase proteins with increased stability and increased retained catalytic activity
KR20140138850A (ko) 2012-03-02 2014-12-04 샤이어 휴먼 지네틱 테라피즈 인크. Ｉｉｉ형 고셔병을 치료하기 위한 조성물 및 방법
KR20190114003A (ko) 2012-03-15 2019-10-08 옥시레인 유케이 리미티드 폼페병의 치료를 위한 방법 및 물질
GB201508025D0 (en) 2015-05-11 2015-06-24 Ucl Business Plc Fabry disease gene therapy
US20220125892A1 (en) 2019-02-01 2022-04-28 OXYRANE UK Ltd. Glucocerebrosidase polypeptides
AU2021245409A1 (en) * 2020-03-29 2022-11-24 Yeda Research And Development Co. Ltd. Variants of beta-glucocerebrosidase for use in treating Gaucher Disease
IL299811A (en) * 2020-07-29 2023-03-01 Freeline Therapeutics Ltd polypeptide

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0370205A2 (fr) * 1988-09-29 1990-05-30 Kyowa Hakko Kogyo Co., Ltd. Polypeptides glycosylés
WO1990007573A1 (fr) * 1988-12-23 1990-07-12 Genzyme Corporation Glucosyl-ceramidase de recombinaison a action enzymatique
WO1993006216A1 (fr) * 1991-09-26 1993-04-01 Oklahoma Medical Research Foundation Proteines de fusion ciblees sur des lysosomes utiles en therapie du sida
US5549892A (en) * 1988-12-23 1996-08-27 Genzyme Corporation Enhanced in vivo uptake of glucocerebrosidase
WO1997004079A1 (fr) * 1995-07-14 1997-02-06 Novo Nordisk A/S Enzyme modifiee a activite lipolytique
US5620884A (en) * 1992-12-10 1997-04-15 Enzon, Inc. Glycolipid enzyme-polymer conjugates

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0370205A2 (fr) * 1988-09-29 1990-05-30 Kyowa Hakko Kogyo Co., Ltd. Polypeptides glycosylés
WO1990007573A1 (fr) * 1988-12-23 1990-07-12 Genzyme Corporation Glucosyl-ceramidase de recombinaison a action enzymatique
US5549892A (en) * 1988-12-23 1996-08-27 Genzyme Corporation Enhanced in vivo uptake of glucocerebrosidase
WO1993006216A1 (fr) * 1991-09-26 1993-04-01 Oklahoma Medical Research Foundation Proteines de fusion ciblees sur des lysosomes utiles en therapie du sida
US5620884A (en) * 1992-12-10 1997-04-15 Enzon, Inc. Glycolipid enzyme-polymer conjugates
WO1997004079A1 (fr) * 1995-07-14 1997-02-06 Novo Nordisk A/S Enzyme modifiee a activite lipolytique

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CANTOR ALAN B ET AL: "Phosphorylation of Asn-linked oligosaccharides located at novel sites on the lysosomal enzyme cathepsin D.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 32, 1992, pages 23357 - 23363, XP002173975, ISSN: 0021-9258 *
DOEBBER T W ET AL: "ENHANCED MACROPHAGE UPTAKE OF SYNTHETICALLY GLYCOSYLATED HUMAN PLACENTAL BETA GLUCO CEREBROSIDASE", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 257, no. 5, 1982, pages 2193 - 2199, XP001002183, ISSN: 0021-9258 *
FULLER MARIA ET AL: "Receptor mediated binding of two glycosylation forms of N-acetylgalactosamine-4-sulphatase.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1406, no. 3, 28 April 1998 (1998-04-28), pages 283 - 290, XP001002494, ISSN: 0006-3002 *
FURBISH F S ET AL: "UPTAKE AND DISTRIBUTION OF PLACENTAL GLUCO CEREBROSIDASE IN RAT HEPATIC CELLS AND EFFECTS OF SEQUENTIAL DEGLYCOSYLATION", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 673, no. 4, 1981, pages 425 - 434, XP001002396, ISSN: 0006-3002 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9220737B2 (en) 2003-04-27 2015-12-29 Protalix Ltd. Plant cell culture expressing human lysosomal proteins and uses thereof

Also Published As

Publication number Publication date
AU2352201A (en) 2001-07-16
EP1246915A2 (fr) 2002-10-09
IL150314A0 (en) 2002-12-01
WO2001049830A2 (fr) 2001-07-12
WO2001049830A9 (fr) 2002-11-07

Similar Documents

Publication Publication Date Title
WO2001049830A3 (fr) Enzymes lysosomales ameliorees et activateurs d"enzymes lysosomales
WO1996021730A3 (fr) Ribozymes comportant un site de liaison de proteine arn
AU2001286636A1 (en) Method for assaying the activity of lysosomal enzymes
IL135578A0 (en) Compositions and methods for treating lysosomal storage disease
BG103977A (en) Low molecular rotamase enzymic activity inhibitors
AU4186199A (en) Method of enhancing lysosomal alpha-galactosidase a
DE69907240D1 (de) Feste phytase-zusammensetzungen
WO1999058690A3 (fr) Molecules d'acide nucleique codant pour des enzymes issues du froment et participant a la synthese de l'amidon
WO2006063055A3 (fr) Conjugues enzymatiques utilises en tant qu'agents detoxifiants
AU7908298A (en) Endo-beta-1,4-glucanases from (saccharothrix)
TW337997B (en) Pharmaceutical preparation containing plasminogen activators
AU3388400A (en) Human glycosylation enzymes
AU1402201A (en) Assay for measuring enzyme activity in vivo
AU3671700A (en) Plant activator, process for producing the same, activation method, activity promoter and method for applying the promoter
AU6539000A (en) Composition and method for treating disease by increasing activated alpha2 macroglobulin in the blood and extravascular tissue
AU7552098A (en) Physiologically active substances tkr2449, process for producing the same, and microorganism
WO2000046245A8 (fr) Proteines antibiotiques humaines
ZA200200128B (en) Novel naphtylsulfonic acids and related compounds as glucose uptake agonists.
WO2004014974A8 (fr) Polymeres contenant un tampon, polymeres contenant un tampon et une enzyme coimmobilises et leurs methodes de synthese
WO1998007716A3 (fr) Agents anticancereux hetero-arotinoides possedant une specificite en matiere de recepteurs et une activite de tgase
WO2001060986A3 (fr) Enzymes hydrolases a activite selective
WO2001046128A3 (fr) Inhibiteurs de tryptase
AU1411200A (en) Novel physiologically active substance, process for producing the same and utilization thereof
AU2001277737A1 (en) Novel substance having physiological activity, process for producing the same, and use
SG74579A1 (en) Enzyme with leudh activity, nucleotide sequence coding therefor and process for the preparation of the enzyme

Legal Events

Date Code Title Description
ENP Entry into the national phase

Ref country code: US

Ref document number: 2001 896896

Date of ref document: 20010629

Kind code of ref document: A

Format of ref document f/p: F

AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 150314

Country of ref document: IL

WWE Wipo information: entry into national phase

Ref document number: 2000987208

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000987208

Country of ref document: EP

AK Designated states

Kind code of ref document: C2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: C2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2000987208

Country of ref document: EP