WO2001031383A1 - Transparent object carrier for use in optical microscopy - Google Patents
Transparent object carrier for use in optical microscopy Download PDFInfo
- Publication number
- WO2001031383A1 WO2001031383A1 PCT/EP2000/010576 EP0010576W WO0131383A1 WO 2001031383 A1 WO2001031383 A1 WO 2001031383A1 EP 0010576 W EP0010576 W EP 0010576W WO 0131383 A1 WO0131383 A1 WO 0131383A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- slide
- film
- line network
- optical microscopy
- transparent
- Prior art date
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
Definitions
- the invention relates to a transparent slide for optical microscopy, with a support side for the microscope object and a visible line network for checking it.
- the slide should be used in particular for microscopic examinations of biological objects.
- glass slide plates In connection with counting chambers for the microscopic counting of cellular elements, glass slide plates are known as slides, which are provided with a line network. The lines are inserted directly into the glass material or applied to the glass material. Observations and counts must be made under sterile conditions.
- DE 41 32 379 C2 discloses a slide for optical microscopy, in which a line network is clamped between two panes in a liquid-tight manner.
- DE 3738982 C2 describes a microscope slide which has grid cutouts which are closed by a self-adhesive film.
- the present invention has for its object to provide a new slide for microscopic examinations, which can be produced with less effort compared to such known slides.
- the object holder according to the invention solving this object is characterized in that the line network is formed on a film which is applied to the side of the object holder facing away from the carrier side.
- slides with a line network can be produced in a simple manner and in particular directly at the microscope station.
- a line network which can be used as a coordinate network.
- the film adheres adhesively or / and electrostatically to the slide or is glued to the slide, preferably using an adhesive which completely wets both the slide plate and the film while avoiding streaking.
- the film is preferably a plastic film that is transparent and allows both reflected light and inverted microscopy.
- the film can be a self-adhesive film, in particular removable from a film carrier.
- a multilayer film if appropriate, can advantageously be stored long-term and, if necessary, processed without a great deal of work.
- the film is expediently removable from a film supply roll, the film then being cut off the roll. It would also be conceivable that the film can be torn off the roll along a perforation.
- the film can be formed from several layers and an outer protective film layer facing away from the specimen slide can be peelable from the remaining film.
- the film can be easily handled when connected to the slide plate. Any soiling caused by fingerprints Microscoping would interfere easily by removing the outer layer of film.
- the film and the slide are matched to one another in the heat coefficient. Deformation of the film relative to the carrier plate, e.g. in the event of heating by a lighting device which could lead to streaking, this is avoided.
- the slide can be the usual rectangular glass slides used as a slide. Slides can of course also be formed by any vessel containing the microscope object.
- the slide is preferably a disposable product. Because the line network can be generated with little effort, there is no economic incentive to reuse it.
- FIG. 1 shows a microscope device according to the invention in a schematic representation
- FIG. 2 shows a slide used in the microscope device of FIG. 1, which is connected to a film according to the invention
- FIG. 3 shows a film provided for connection to the slide of FIG. 2
- FIG. 4 shows a detail of the film from FIG. 3 in a top view.
- Reference number 1 denotes a schematically illustrated imaging optics of a microscope with an optical axis 2.
- the imaging optics 1 magnifies microscopic objects arranged on a slide 3 at 4, e.g. Cell cultures.
- the slide 3 is a thin glass plate, as is usually used as a slide in light microscopes.
- a film 7 is glued onto the slide, as indicated by arrows 5 and 6, onto which a slide 7 is attached Coordinate network 8 is printed.
- an adhesive is used, which wets both the slide 3 and the film 7 evenly, so that a streaking that disrupts the optical image of the object and the coordinate network is avoided.
- the film 7 glued onto the slide 3 is self-adhesive and was removed from a slide 9 shown in FIG. 3 before being glued onto the slide 3.
- the section of film carrier 9 and film 7 shown in FIG. 3 can be unwound from a supply roll on which individual sections are separated by perforations and can be torn off the roll.
- the coordinate network printed on the film 7 has large fields identified by numbers and lower case letters and small fields identified by numbers and upper case letters. Both the small fields and the large fields are square. In the exemplary embodiment shown, the side length of the large fields is 10 mm and the side length of the small fields is 1 mm.
- the section shown in FIG. 4 corresponds approximately to the object field imaged by the imaging device 2. The coordinate network thus allows a targeted search for an examination object arranged somewhere on the slide, provided that its coordinates are known.
Abstract
Description
Claims
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00975933A EP1234205A1 (en) | 1999-10-28 | 2000-10-27 | Transparent object carrier for use in optical microscopy |
JP2001533461A JP2003513301A (en) | 1999-10-28 | 2000-10-27 | Transparent object carrier for light microscopy |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE1999152139 DE19952139C1 (en) | 1999-10-28 | 1999-10-28 | Transparent object carrier for optical microscope has adhered transparent plastics foil providing line grid used as coordinate refernece grid for examination of microscope object |
DE19952139.5 | 1999-10-28 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2001031383A1 true WO2001031383A1 (en) | 2001-05-03 |
Family
ID=7927312
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2000/010576 WO2001031383A1 (en) | 1999-10-28 | 2000-10-27 | Transparent object carrier for use in optical microscopy |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1234205A1 (en) |
JP (1) | JP2003513301A (en) |
DE (1) | DE19952139C1 (en) |
WO (1) | WO2001031383A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6597500B1 (en) | 1999-11-22 | 2003-07-22 | Genetix Limited | Adhesive label with grid for microscope slide |
DE102004049323B4 (en) * | 2003-10-31 | 2008-05-21 | Leica Microsystems Cms Gmbh | Microscope slides for educational purposes |
WO2017009673A1 (en) * | 2015-07-16 | 2017-01-19 | National Hellenic Research Foundation | Reference and calibration grid for medical diagnostic microscopy |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE20211509U1 (en) * | 2002-07-13 | 2003-12-24 | Evotec Oai Ag | Sample carrier used as titer plate or micro-titer plate for investigating chemical and/or biological samples in medium and/or high throughput screening, comprises several recesses closed by base plate with a mark in region of recesses |
US7329537B2 (en) | 2003-04-17 | 2008-02-12 | Nexcelom Bioscience, Llc | Micro-pattern embedded plastic optical film device for cell-based assays |
BRPI0617836A2 (en) * | 2005-10-26 | 2011-08-09 | Lee H Angros | microscope slide cover plate, microscope slide cover plate set, and method of forming a microscope slide cover plate set |
DE102006036172B4 (en) * | 2006-07-31 | 2018-11-22 | Carl Zeiss Microscopy Gmbh | Optical arrangement for the sequential positioning of working fields on an object |
JP2009036969A (en) * | 2007-08-01 | 2009-02-19 | Nikon Corp | Cover glass, slide glass, preparation, observation method, and microscopic device |
JP2009237277A (en) * | 2008-03-27 | 2009-10-15 | Nippon Zeon Co Ltd | Container for microscopic observation |
AT13213U1 (en) * | 2012-05-04 | 2013-08-15 | Kdg Mediatech Ag | Microscope Help |
JP5878490B2 (en) * | 2013-02-08 | 2016-03-08 | 日本ゼオン株式会社 | Microscope observation container |
WO2022097356A1 (en) * | 2020-11-09 | 2022-05-12 | 有限会社ウィン | Optical microscope system based on ergonomic field of view |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3912360A (en) * | 1974-04-24 | 1975-10-14 | Frank L Beckel | Microscopic field marker |
DE2450797A1 (en) * | 1974-10-25 | 1976-05-06 | Goldsmith | Microscope object slide - designed for cytological and haemotological tests and has transparent cover plate over object |
FR2542876A1 (en) * | 1983-03-14 | 1984-09-21 | Jouet Cie Gle | Composite for the production of a microscope slide |
GB2163866A (en) * | 1984-08-30 | 1986-03-05 | Erba Farmitalia | Grating film for quantitative or semi-quantitative microscopic evaluation of biological specimens |
EP0674201A1 (en) * | 1994-03-22 | 1995-09-27 | Roche Diagnostics GmbH | Slide for microscopic processing of fluid samples |
US5812312A (en) * | 1997-09-04 | 1998-09-22 | Lorincz; Andrew Endre | Microscope slide |
WO1999053357A1 (en) * | 1998-04-09 | 1999-10-21 | 3M Innovative Properties Company | Adhesive cover slip and method for microscopy |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3738982A1 (en) * | 1987-04-30 | 1988-11-17 | Diagen Inst Molekularbio | Method for the determination of proteins, protein structures and nucleic acids from body fluids |
DE4132379A1 (en) * | 1991-09-28 | 1993-04-08 | Kernforschungsz Karlsruhe | SUBSTRATE FOR CELL CULTURES AND CULTURE OF CELLS OR CELL AGGREGATES |
US5349436A (en) * | 1992-12-02 | 1994-09-20 | Harry Fisch | Biological assembly |
-
1999
- 1999-10-28 DE DE1999152139 patent/DE19952139C1/en not_active Expired - Lifetime
-
2000
- 2000-10-27 EP EP00975933A patent/EP1234205A1/en not_active Withdrawn
- 2000-10-27 JP JP2001533461A patent/JP2003513301A/en active Pending
- 2000-10-27 WO PCT/EP2000/010576 patent/WO2001031383A1/en not_active Application Discontinuation
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3912360A (en) * | 1974-04-24 | 1975-10-14 | Frank L Beckel | Microscopic field marker |
DE2450797A1 (en) * | 1974-10-25 | 1976-05-06 | Goldsmith | Microscope object slide - designed for cytological and haemotological tests and has transparent cover plate over object |
FR2542876A1 (en) * | 1983-03-14 | 1984-09-21 | Jouet Cie Gle | Composite for the production of a microscope slide |
GB2163866A (en) * | 1984-08-30 | 1986-03-05 | Erba Farmitalia | Grating film for quantitative or semi-quantitative microscopic evaluation of biological specimens |
EP0674201A1 (en) * | 1994-03-22 | 1995-09-27 | Roche Diagnostics GmbH | Slide for microscopic processing of fluid samples |
US5812312A (en) * | 1997-09-04 | 1998-09-22 | Lorincz; Andrew Endre | Microscope slide |
WO1999053357A1 (en) * | 1998-04-09 | 1999-10-21 | 3M Innovative Properties Company | Adhesive cover slip and method for microscopy |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6597500B1 (en) | 1999-11-22 | 2003-07-22 | Genetix Limited | Adhesive label with grid for microscope slide |
DE102004049323B4 (en) * | 2003-10-31 | 2008-05-21 | Leica Microsystems Cms Gmbh | Microscope slides for educational purposes |
WO2017009673A1 (en) * | 2015-07-16 | 2017-01-19 | National Hellenic Research Foundation | Reference and calibration grid for medical diagnostic microscopy |
Also Published As
Publication number | Publication date |
---|---|
JP2003513301A (en) | 2003-04-08 |
DE19952139C1 (en) | 2000-12-21 |
EP1234205A1 (en) | 2002-08-28 |
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