JP5878490B2 - Microscope observation container - Google Patents

Description

  The present invention relates to a microscope observation container used for observing an inspection object using a microscope.

  In university or corporate laboratories, biological cells, biological tissue fragments, microorganisms, bacteria, etc., or cultures thereof, or changes in these drugs or genes injected over time (morphological changes, migration, etc.) Are observed with a microscope, and biochemical and pharmaceutical researches have been conducted. There are various types of microscopes such as a stereo microscope, a fluorescence microscope, a phase contrast microscope, a differential interference microscope, and the like, which are appropriately selected and used according to the purpose of the research and the properties of the object. Recently, in addition to visual observation through an eyepiece, the microscope picks up an image with an image pickup device such as a CCD and displays it on a monitor, or stores it as image data in a personal computer or the like. Image processing and the like can be performed.

  When observing such time-dependent changes of cells or the like with a microscope, a microscope observation container that also serves as a culture container is generally used. As a microscope observation container, it is provided with an accommodating portion for accommodating an inspected object such as a cell, and this accommodating portion has a plate-like portion constituting the bottom surface and a cylindrical portion constituting the side wall. As a container having a single housing portion, there are a so-called cell culture dish and a microplate in which a plurality of housing portions are arranged in an array (matrix).

  Since at least the plate-like portion of the housing portion needs to transmit illumination light, it is formed of a material having high light transmittance, and has a plate thickness thinner than a value determined in relation to the focal length of the objective lens of the microscope, and the like. Need to be done. As a plate for microscopic observation, a glass plate having good optical properties and capable of maintaining a desired strength even with a relatively thin plate thickness is used as the plate-like portion, and the remaining portion such as the cylindrical portion is made of resin. What was formed is known (for example, refer to Patent Document 1 or Patent Document 2 below). However, recently, resin materials have been developed that have optical properties close to or exceeding that of glass and sufficient mechanical strength, and the resin is light and easy to mold. It is now possible to manufacture the entire container containing the resin with resin.

  By the way, when observing a time-dependent change of an inspection object using such a microscope observation container, a container containing the inspection object is installed on the stage of the microscope, and the inspection object on the plate-like portion is placed. After magnifying a part and observing (imaging) at multiple locations, remove it from the stage, place it on the stage again after a while, and repeat the operation several times to observe at the same position and magnification Will be implemented. In this case, when the amount of morphological change or movement of cells or the like is measured, or when comparing the observation images before and after being overlapped by image processing, a reference regarding the position on the plate-like portion is required.

  As such a position reference, Patent Document 1 or Patent Document 2 discloses a structure in which grid-like grid coordinates including a plurality of latitudes and meridians are provided on a plate-like portion. Outside the grid coordinates, alphabets and numerals are displayed as symbols for distinguishing each latitude and longitude from other latitudes and longitudes (see, for example, FIG. 6 of Patent Document 1 and FIG. 3 of Patent Document 2). ).

  However, as described above, when observing with a microscope, since an arbitrary part of the inspection object on the plate-like portion is observed in an enlarged state, the symbols displayed outside the grid coordinates are displayed with a microscope during the magnifying observation. If there is no symbol in the microscope field of view, set the magnification to a low magnification so that the entire grid coordinates including the symbol are within the microscope field of view, and then observe After confirming and adjusting the power position, it is necessary to set the magnification again at the desired magnification for observation, and such an operation is complicated and requires a long time for observation.

  In addition, in the state of observing at a magnification, even if the microscopic field includes latitude lines and meridians, the latitude lines and meridians are often indistinguishable from other latitude lines and other meridians. In some cases, the position cannot be confirmed and the observation is performed at an incorrect position, and when the observation image is processed as image data, the position cannot be specified similarly.

  The above problem will be described more specifically below, taking as an example the case of observing an object to be inspected that is located at the center of the grid coordinates. That is, first, the coordinate position of the object to be inspected is confirmed with the magnification of the microscope set to a low magnification. Next, in the state where the magnification of the microscope is set to an enlargement magnification, for example, the vertical position of the grid coordinate is specified by fixing the vertical position of the stage while looking at the identification symbol displayed outside the grid coordinate. . Next, in the magnification setting state, since the identification symbol indicating the horizontal position of the grid coordinate does not enter the microscope field of view, the number of grids from the end of the grid coordinate is counted, and the horizontal direction of the grid coordinate The stage is moved along the position of the stage, the position in the lateral direction of the stage is specified and the position in the lateral direction of the stage is fixed, and the inspection object is observed in this state. However, when specifying the horizontal position of the stage, it is necessary to count the number of grids, and misunderstanding of the count number, incorrect count, and the like can frequently occur. In addition, when the object to be inspected is a cell, the outer shape of the cell can change over time, so just observing the object to be inspected can determine whether or not the same object is being observed. Can not. For this reason, according to the said procedure, there exists a problem that observation of a to-be-inspected object is very difficult.

  The present invention has been made in view of the above points, and provides a microscope observation container capable of easily recognizing and specifying the position under observation when observing at a magnification. Objective.

JP 2001-17157 A JP 2004-201695 A

According to the present invention, a microscope observation container for observing an object to be inspected using a microscope, comprising a plate-like portion that supports the object to be inspected, the entirety including the plate-like portion being an acrylic resin, a polycarbonate It is integrally formed of resin, polystyrene, or resin having an alicyclic structure, and the plate-like portion is provided with a grid composed of a plurality of latitudes and meridians arranged at a predetermined interval. Formed by forming a convex portion corresponding to the grid line on the mold, and transferring the concave portion by pressing the convex portion against the plate-like portion, the grid lines that are the latitude and the meridian, and the inside of the grid, At least one of these is provided with a microscope observation container provided so that a mark that can identify the absolute position of the grid is always included in an enlarged image .

In the present invention, as the resin , a resin having an alicyclic structure can be used. In the present invention, the interval between the meridians can be less than 200 μm.

  In addition, as the mark, those expressed by features for distinguishing the latitude line and the meridian from each other can be used. In this case, as the characteristics, those expressed by the number of lines, the thickness of the lines, the types of lines, the symbols attached to the lines, and combinations of at least two of these can be used. Further, as the feature, the latitude line and / or the meridian may be expressed by forming the line itself with a symbol that can identify the line itself.

  Moreover, in this invention, the said plate-shaped part can be provided with the cylindrical part which has an opening in the upper part on the bottom, and in addition, the lid | cover which has a columnar part inserted from the said opening of the said cylindrical part is provided. Can do.

  According to the microscope observation container of the present invention, the absolute position of the grid is set in at least one of the inside of each grid provided on the plate-like portion that supports the object to be inspected and the parallels and meridians that form the grid. Since the identifiable mark is provided, there is no need to set the low magnification once and confirm, as in the prior art, and the position during observation can be easily recognized when observing at the magnification. -There is an effect that it can be specified.

  In addition, when image processing is performed on the magnified image, the image always includes a mark for identifying the position. Therefore, the observation position does not exactly match the preceding and subsequent image data. In addition, it is possible to accurately superimpose the preceding and succeeding images at the time of image processing by the mark. Therefore, there is no need to strictly match the front and rear observation positions, and the work can be facilitated.

It is side sectional drawing which shows an example of the container for microscope observation of embodiment of this invention. It is a top view of the container main body of the container for microscope observation of FIG. It is a sectional side view which shows the other example of the container for microscope observation of embodiment of this invention. It is a top view of the container main body of the container for microscope observation of FIG. It is a figure which shows the 1st structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. It is a figure which shows the 2nd structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. It is a figure which shows the 3rd structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. It is a figure which shows the 4th structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. It is a figure which shows the 5th structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. It is a figure which shows the 6th structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. It is a figure which shows the 7th structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. It is a figure which shows the 8th structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. It is a figure which shows the 9th structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. It is a figure which shows the modification of the 9th structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. It is a figure which shows the other modification of the 9th structure of the mark arrange | positioned at the plate-shaped part of embodiment of this invention. In the 1st-9th structure of embodiment of this invention, it is a figure which shows the case where a scale is written together.

  Hereinafter, a microscope observation container according to an embodiment of the present invention will be described with reference to the drawings.

[Overall configuration of container]
FIG. 1 is a side sectional view showing an overall configuration of an example of a microscope observation container according to an embodiment of the present invention, and FIG. 2 is a plan view showing a configuration of a container main body of the microscope observation container of FIG.

  The microscope observation container 11 is a container called a cell culture dish or the like, and includes a container body 12 and a lid 13. The container main body 12 is a substantially cylindrical member (petri dish) whose upper surface is open and whose lower surface is closed, and a medium containing cells or the like as a test object is accommodated in a substantially central portion of the bottom 12a of the container main body 12. The accommodating part 14 is arrange | positioned. The accommodating portion 14 is configured by forming a concave portion at a substantially central portion of the bottom portion 12a of the container body 12, and the bottom portion of the concave portion serves as a plate-like portion 14a for supporting (or attaching) the inspection object. The side surface of the recess is a cylindrical portion 14 b that constitutes the side wall of the accommodating portion 14.

  The plate thickness (wall thickness) of the plate-like portion 14a is a predetermined thickness in relation to the focal length of the objective lens of the microscope used when observing using the container 11. As shown in FIG. 2, the plate-like portion 14a is provided with a mark M for recognizing and specifying the position in the plate-like portion 14a during magnified observation. This mark M will be described in detail later. A step 12 c is formed in the vicinity of the opening of the side wall 12 b of the container body 12.

  The lid body 13 is formed of a substantially disk-like member that detachably closes the upper surface opening of the container main body 12, and an annular side wall portion 13 a is formed so as to fit into the step 12 c portion of the container main body 12. Has been. In addition, although illustration is abbreviate | omitted, in the state which closed the opening of the container main body 12 with the cover body 13 in the lower surface of the cover body 13, for absorbing the oxygen of the space defined inside and adjusting the density | concentration A pocket or the like for accommodating the oxygen absorbent may be provided. This is because it may be desirable to culture and observe living cells and the like under a low oxygen concentration (for example, about 5%) as in the body.

  FIG. 3 is a side sectional view showing the overall configuration of another example of the microscope observation container according to the embodiment of the present invention, and FIG. 4 is a plan view showing the configuration of the container main body of the microscope observation container of FIG. .

  The microscope observation container 21 is a container called a microplate or the like, and includes a container main body 22 and a lid body 23. The container body 22 is configured by arranging, on the bottom plate 22a, a plurality of accommodating portions 24 that accommodate a culture medium containing cells or the like as test objects. Here, as an example, a total of eight accommodating portions 24 of 2 × 4 are provided. However, the number of the accommodating parts 24 may be less than 8 (for example, 4 of 2 × 2) or 9 or more. Each accommodating portion 24 has a substantially cylindrical tubular portion 24b whose upper surface is open and whose lower surface is closed, and the bottom of the accommodating portion 24 (the portion corresponding to the accommodating portion of the bottom plate 22a) is the object to be inspected. It is a plate-like portion 24a for supporting (or attaching).

  The plate thickness (thickness) of the plate-like portion 24a (that is, the bottom plate 22a) is a predetermined thickness in relation to the focal length of the objective lens of the microscope used when observing using the container 21. Yes. Further, as shown in FIG. 4, each plate-like portion 24a of each accommodating portion 24 has a plate at the time of magnified observation, like the microscope observation container 11 described above with reference to FIGS. A mark M for recognizing and specifying the position in the shape portion 24a is arranged. This mark M will be described in detail later.

  The lid body 23 is formed of a substantially rectangular member that detachably closes the upper surface opening of each housing portion 24 of the container body 22, and a portion in the vicinity of the opening side inside the tubular portion 24 b of each housing portion 24. A columnar portion 23a that is loosely fitted to each other is disposed corresponding to each accommodating portion 24, and has a convex portion 23b that is formed to fit outside the cylindrical portion 24b. The columnar portion 23a is inserted with a slight gap from the cylindrical portion 24b in a state where the lid body 23 is attached so as to close the opening of each accommodating portion 24, and is accommodated in the accommodating portion 24, and the plate-like portion 24a. Meniscus that may occur at the interface (upper surface) of the liquid when the medium supported on the liquid is liquid is eliminated by slightly inserting the tip surface of the cylindrical portion 23a into the liquid. It is. When a meniscus is generated, the vicinity of the interface of the liquid acts as a lens, which may cause an error in microscopic observation, and such a columnar portion 23a can prevent this.

  In addition, the structure of the container shown in FIG. 1 and FIG. 2, or FIG. 3 and FIG. 4 is an illustration, Comprising: Another structure may be sufficient.

[Container forming material]
Although these container main bodies 12 and 22 and the lids 13 and 23 may be manufactured using glass, it is preferable to use a resin because they are lightweight and easy to mold. Examples of the transparent resin to be used include acrylic resins, polycarbonate resins, polystyrene, and resins having an alicyclic structure. Among these, the resin having an alicyclic structure has good fluidity, and even a thin plate thickness (thickness) required for the plate-like portion due to the relationship with the focal length of the objective lens of the microscope, etc. It is preferable because sufficient mechanical strength can be realized and optical characteristics required for the light transmitting portion including the plate-shaped portion of the container, such as high light transmittance, flatness, uniformity, and low birefringence, can be favorably realized. In addition, since the resin having an alicyclic structure has small autofluorescence due to the material, there is no problem even in the use for fluorescence observation, and it is preferable because it has excellent chemical resistance.

  The resin having an alicyclic structure is a resin having an alicyclic structure in the main chain and / or side chain. From the viewpoint of mechanical strength and the like, a resin containing an alicyclic structure in the main chain is particularly preferable. Examples of the alicyclic structure include a saturated cyclic hydrocarbon (cycloalkane) structure and an unsaturated cyclic hydrocarbon (cycloalkene) structure. From the viewpoint of mechanical strength and the like, a cycloalkane structure or a cycloalkene structure is preferable, and among them, a cycloalkane structure is most preferable. The number of carbon atoms constituting the alicyclic structure is not particularly limited, but is usually 4 to 30, preferably 5 to 20, more preferably 5 to 15 in the range of mechanical strength and Properties such as moldability are highly balanced and suitable. The ratio of the repeating unit having an alicyclic structure in the resin having an alicyclic structure is usually 50% by weight or more, preferably 70% by weight or more, more preferably 90% by weight or more.

  Specific examples of the resin having an alicyclic structure include (1) ring-opening polymer of norbornene-based monomer and ring-opening of norbornene-based monomer and other monomers capable of ring-opening copolymerization Norbornene polymers such as copolymers, addition products of these hydrogenated products, norbornene monomers, and addition copolymers of norbornene monomers with other monomers copolymerizable therewith (2) monocyclic olefin polymer and hydrogenated product thereof; (3) cyclic conjugated diene polymer and hydrogenated product thereof; (4) polymer of vinyl alicyclic hydrocarbon monomer and Copolymers of vinyl alicyclic hydrocarbon monomers and other monomers copolymerizable therewith, as well as double bonds of these hydrogenated products and polymers of vinyl aromatic monomers Hydrogenated (including aromatic rings) and vinyl aromatics And vinyl alicyclic hydrocarbon polymers such as hydrogenated products of double bonds (including aromatic rings) of copolymers of monomers and other monomers copolymerizable therewith. .

  Among these, from the viewpoints of optical properties and mechanical strength, norbornene-based polymers and vinyl alicyclic hydrocarbon-based polymers are preferable, ring-opening polymer hydrogenated norbornene-based monomers, norbornene-based single monomers Hydrogenation product of ring-opening copolymer of polymer and other monomer capable of ring-opening copolymerization, hydrogenation of double bond part (including aromatic ring) of vinyl aromatic monomer polymer And a hydrogenated product of a double bond portion (including an aromatic ring) of a copolymer of a vinyl aromatic monomer and another monomer copolymerizable therewith.

[Composition of landmarks]
On the front surface (surface supporting the inspection object) or the back surface (surface opposite to the surface supporting the inspection object) of the plate-like portions 14a and 24a on which the inspection objects of the container bodies 12 and 22 are supported, In order to discriminate the position in the plate-like portions 14a and 24a being magnified and observed from other positions in the plate-like portions 14a and 24a in a state in which an arbitrary part in the shape-like portions 14a and 24a is enlarged and observed. The mark M is arranged. As this mark M, for example, a structure (first structure) as shown in FIG. 5 can be used.

  The mark M shown in FIG. 5 arranges a plurality of latitude lines (horizontal lines) Y0 to Y4... And a plurality of meridian lines (vertical lines) X0 to X4. As a feature for discriminating from other latitudes or meridians, an address is arranged in the vicinity of the intersection as a symbol IM indicating the position of the intersection of the latitude and longitude. Here, solid lines are used as latitudes and meridians, and the line width is about 1 μm.

  In FIG. 5, a rectangular frame with a reference symbol Fi indicates the observation field (or imaging field) of the microscope when the containers 11 and 21 are placed on the stage of the microscope and observed at a predetermined magnification. Show. Note that the position of the observation visual field Fi in the figure is an example, and can be moved to any position on the plate-like portions 14a and 24a by moving the microscope stage automatically or manually. The size of the observation visual field Fi depends on the specifications of the microscope, but since the size of the cell as the inspection object is generally about 100 μm or less, the observation magnification is set to 60 times, and the vertical 150 μm × 200 μm on the test surface. Or about 75 μm long × 100 μm wide are used.

  The arrangement intervals of the latitude lines Y0 to Y4... And the meridians X0 to X4 (hereinafter also referred to as grid lines) are set at the largest magnification assumed to be used for observation of the inspection object. The intervals are set such that at least one of the intersections falls within the observation visual field Fi, that is, at least one latitude line and at least one meridian fall within the observation visual field Fi. For example, assuming that the maximum magnification is 60 times, the interval between latitude lines is set to less than 150 μm, and the interval between meridians is set to less than 200 μm. Here, as an example, the interval between the latitude and longitude lines is the same and is set to 140 μm, which is slightly smaller than 150 μm.

  The address as the symbol IM is expressed by a four-digit number in the figure, and among the four digits, the upper two digits identify a latitude line and the lower two digits identify a meridian. With this notation, 100 lines from 00 to 99 can be identified for both latitude and meridian. The size of the symbol IM is set to a size that is as small as possible so as not to hinder observation and is identifiable in relation to the observation magnification. Specifically, as an example, the observation magnification is about 60 times, the size of one character can be about 10 μm square, and the space between characters can be about 3 μm.

  As a method of forming the grid lines and the symbols IM on the plate-like portions 14a and 24a, they can be formed by nanoimprinting or photolithography. Nanoimprinting is a method in which a convex portion corresponding to a grid line and a symbol IM is formed on a mold and is pressed against the plate-like portions 14a and 24a to form a concave portion. Photolithography is a method for forming a thin film. The grid lines and the symbol IM are patterned by mask exposure, etching, and the like. However, the method of forming the grid line and the symbol IM is not limited to these, and other methods may be used.

Next, some modified examples of the mark M will be described. FIG. 6 shows a second configuration of the mark. This second configuration is obtained by changing the notation of the symbol IM according to the first configuration shown in FIG. It is a thing. The notation of the grid lines is the same as in FIG.
With this notation, it is possible to identify 26 letters from alphabets A to Z for both latitude and meridians. In addition, the notation method of the symbol IM may be based on characters or figures other than the alphabet in addition to those based on the numbers and alphabets as shown in FIG. Moreover, you may use what combined 2 or more of a number, a character, a figure, etc.

In FIG. 5 or FIG. 6, the grid line is displayed by a single line type (solid line), and the grid line can be identified by combining it with the symbol IM. On the other hand, in the third configuration shown in FIG. 7, the grid line is not identified using such a symbol IM, but the type (line type) of the grid line itself can be different from each other. I am doing so. In the third configuration, a solid line and a dotted line are used as the grid line, and the dotted line can be distinguished from other dotted lines with different pitches (periods) between the point portion and the intermittent portion. As a line type, in addition to a solid line and a dotted line, a chain line such as a one-dot chain line or a two-dot chain line can also be used.
In the case of the chain line, as in the case of the dotted line, the long line part, the short line part, and the intermittent part can be distinguished from each other by different periods.

  FIG. 8 shows a fourth configuration of the mark. In the fourth configuration, solid lines are used as grid lines, and the line widths are different from each other so that they can be distinguished from each other. It is difficult to identify a large number of expressions by changing the line width alone, but when the number of grid lines is not so large, it is possible to identify only by changing the line width. The notation based on the line type shown in FIG. 7 and the notation based on the line width shown in FIG. 8 may be combined, thereby enabling further identification.

  FIG. 9 shows a fifth configuration of the mark. In this fifth configuration, the grid lines are represented by solid lines, and the same lines as the grid lines are added to each other by a bundle of a plurality of lines. It can be identified. If the number of multiple lines that make up one line bundle increases, it may be difficult to see, so if there are many grid lines, for example, combining Roman lines with lines of different line types and line widths It is good to use the notation. The line width of the lines constituting the line bundle can be about 1 μm, and the interval between adjacent lines can be about 2 μm.

  FIG. 10 shows a sixth configuration of the mark. This sixth configuration is an improvement of the notation method using the line bundle of FIG. 9 and is a reference with a solid line at the center of the plate-like bodies 14a and 24a. The latitude line Y2 and the meridian X2 are shown, and the number of lines constituting the line bundle is increased each time the reference latitude line and the meridian are moved up and down or left and right. Accordingly, the latitude lines Y1 and Y3, Y0 and Y4 are represented by the same line bundle, and the meridians X1 and X3 and X0 and X4 are represented by the same line bundle. In this case, an intersection of the same form appears. For example, the intersection of the latitude line Y1 and the meridian X1, the intersection of the latitude line Y1 and the meridian X3, the intersection of the latitude line Y3 and the meridian X1, and the intersection of the latitude line Y3 and the meridian X3 cannot be distinguished from each other. Therefore, in FIG. 10, identification can be performed by attaching a symbol IM in the vicinity of the intersection of the latitude line and the meridian.

  The symbol IM here is a single black dot (dot). The black dots are distinguished from each other depending on which of the first to fourth quadrants, with the intersection point as the origin, the latitude line as the horizontal axis, and the meridian as the vertical axis. For example, the black point attached to the intersection of the latitude line Y1 and the meridian X3 is in the first quadrant, the black point attached to the intersection of the latitude line Y1 and the longitude line X1 is in the second quadrant, and the black point attached to the intersection of the latitude line Y3 and the longitude line X1. Are identified in the third quadrant, and the black point added to the intersection of the latitude line Y3 and the meridian X3 is assigned to the fourth quadrant, thereby identifying each other. When such a notation is used, if the containers 11 and 12 are erroneously installed on the microscope stage in a state of being rotated 90 °, 180 ° or 270 °, the error cannot be identified, which is inconvenient. It is. In order to cope with this case, in FIG. 10, a direction display (arrow) DM indicating the direction is displayed to prevent the container from being rotated and installed on the stage of the microscope.

  In addition, since granules and cell nuclei made of membrane components exist in the cell, depending on the diameter of the black spot as the symbol IM, it may be difficult to distinguish from these, so the diameter of the black spot used is It is preferable that the diameter is sufficiently larger than such a granule and the diameter is sufficiently smaller than the cell nucleus so that they can be distinguished from these. The diameter of the black spot can be set to about several μm, for example. In addition, the symbol IM is not such a black dot, and is a figure that does not exist in the cell or is small in number (for example, a triangle or a quadrangle), a geometric pattern, or a character (for example, an L-shape). If used, it can be clearly distinguished from the pattern present in the cells, which is convenient.

  FIG. 11 shows a seventh configuration of the mark. In this seventh configuration, a plurality of intermittent portions are formed on the latitude lines and meridians so as to correspond to each grid, and the latitude lines or meridians are replaced with other latitude lines or meridians. Numbers are arranged in the intermittent portions as symbols for distinguishing from each other. In addition, a symbol is not restricted to a number, You may use an alphabet, another figure, or these combination. Further, the formation of the intermittent portion and the marking of the symbols may not be performed for all latitude lines or meridians, and may be performed every other line, every other line, or the like.

  FIG. 12 shows an eighth configuration of the mark. In the eighth configuration, even lines of latitude and meridians are relatively thin solid lines, and odd columns are relatively thick to distinguish them from the even columns. A plurality of intermittent portions are formed so as to correspond to each grid only on odd-numbered parallels and meridians, and numbers are used as symbols for distinguishing the parallels or meridians from other parallels or meridians. Each is arranged. Although not particularly limited, it is preferable to align the line widths of thick latitude lines or meridians in even columns with the numbers as symbols. In addition, a symbol is not restricted to a number, You may use an alphabet, another figure, or these combination. Further, the odd-numbered columns may be thick lines, the even-numbered columns may be thin lines, or the intermittent portions may be formed only on the thin lines to arrange the symbols.

  FIG. 13 shows a ninth configuration of the mark. In this ninth configuration, the parallel lines and meridians are not represented by line segments, but symbols formed by combinations of numbers and alphabets are arranged in a straight line. expressing. In FIG. 13, the character strings constituting each latitude line or each meridian are in the same expression format, and the symbols constituting each grid are different in order to distinguish the grid from other grids. In FIG. 13, the direction of the characters composing the latitude line and the characters composing the meridian has a 90-degree relationship. The symbols are not limited to numbers or combinations of alphabets, and numbers, alphabets, other characters and figures may be used alone, or a combination thereof. In addition, the orientation of the characters constituting the latitude and the meridians is not limited to 90 degrees, and may be the same or 180 degrees.

  FIG. 14 shows a modification of the ninth configuration shown in FIG. 13. In FIG. 14, symbols (character strings) constituting each latitude line or each meridian are made different for each latitude line or meridian, and for each grid. It is also different. Further, in FIG. 14, the direction of the characters composing the latitude line and the characters composing the meridian are the same. The symbols are not limited to numbers or combinations of alphabets, and numbers, alphabets, other characters and figures may be used alone, or a combination thereof. In addition, the direction of the characters composing the latitude line and the characters composing the meridian is not limited to the same direction, and may be 90 degrees or 180 degrees.

  FIG. 15 shows another modification of the ninth configuration shown in FIG. 13. In FIG. 15, the symbols (character strings) constituting the latitude lines and meridians are the same character enumeration, and each latitude line or each meridian Each letter is different. In FIG. 15, numerals are used for the latitude lines and alphabets are used for the meridians so that the latitude and meridians can be identified. In FIG. 15, the direction of the characters composing the latitude line and the characters composing the meridian are the same. Note that the symbols are not limited to numbers or alphabets, and numbers, alphabets, other characters and figures may be used alone, or a combination thereof. In addition, the direction of the characters composing the latitude line and the characters composing the meridian is not limited to the same direction, and may be 90 degrees or 180 degrees.

  In the first to ninth configurations of the above-described marks, as shown in FIG. 16, the scale (scale) indicating the length and the dimension (length) of the scale may be written together with numerals in each grid. Good. In the figure, the dimension of the scale is 25 μm. However, only the scale (scale) is displayed, and the scale dimensions may not be indicated. In the sixth configuration shown in FIG. 10, this scale may be displayed in the vicinity of the intersection of the latitude line and the meridian instead of the black point (dot) described above. Further, the scale (and its dimensions) may not be marked for every grid, and every other grid, every other grid, or the like may be used.

  The embodiment described above is described for facilitating the understanding of the present invention, and is not described for limiting the present invention. Therefore, each element disclosed in the above-described embodiment is intended to include all design changes and equivalents belonging to the technical scope of the present invention.

11, 21 ... Microscope observation containers 12, 22 ... Container bodies 13, 23 ... Lid bodies 14, 24 ... Housing parts 14a, 24a ... Plate-like parts 14b, 24b ... Cylindrical parts M ... Marks X0 to X4 ... Meridians Y0 Y4 ... Parallel IM ... Symbol (address, black dot)
Fi: Observation field (imaging field)
DM: Direction display

Claims (7)

A microscope observation container for observing an object to be inspected using a microscope,
A plate-like portion for supporting the object to be inspected;
The entirety including the plate-like portion is integrally formed of an acrylic resin, a polycarbonate resin, polystyrene, or a resin having an alicyclic structure,
The plate-like portion is provided with a grid constituted by a plurality of latitudes and meridians arranged at predetermined intervals and a mark capable of identifying the absolute position of the grid ,
The mark that can identify the grid and the absolute position of the grid is formed as a recess in the plate-like portion ,
The meridian interval is less than 200 μm,
When at least one of the latitude line and the grid line that is the meridian and the inside of the grid is magnified to the extent that there is at least one intersection of the latitude line and the meridian, the grid of the grid in the magnified image is observed. A microscope observation container provided so as to always include a mark capable of identifying an absolute position.   The container for microscopic observation according to claim 1, wherein the resin is made of a resin having an alicyclic structure. The container for microscope observation according to claim 1 or 2 , wherein the mark is expressed by a feature for distinguishing the latitude line and the meridian from each other. The container for microscope observation according to claim 3 , wherein the feature is expressed by the number of lines, the thickness of the lines, the type of lines, the symbols attached to the lines, or a combination of at least two of these. 5. The microscope observation container according to claim 3 , wherein the feature is expressed by configuring the latitude line and / or the meridian in a linear shape by a symbol that can identify the line itself. The container for microscope observation as described in any one of Claims 1-5 provided with the cylindrical part which uses the said plate-shaped part as a bottom face and has an opening in the upper part. The container for microscope observation of Claim 6 provided with the cover body which has a columnar part inserted from the said opening of the said cylindrical part.

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