WO2000078989A1 - Procedimiento de obtencion de acido 7-aminodes-acetoxicefalosporanico (7-adca) - Google Patents
Procedimiento de obtencion de acido 7-aminodes-acetoxicefalosporanico (7-adca) Download PDFInfo
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- WO2000078989A1 WO2000078989A1 PCT/ES2000/000211 ES0000211W WO0078989A1 WO 2000078989 A1 WO2000078989 A1 WO 2000078989A1 ES 0000211 W ES0000211 W ES 0000211W WO 0078989 A1 WO0078989 A1 WO 0078989A1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
- C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
- C12N9/0024—D-Amino acid oxidase (1.4.3.3)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
- C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
Definitions
- the invention is ascribed to the production of 7-ADCA, or its intermediate compounds penicillin N or deacetoxycephalosporin C by fermentation of transformed Acremonium chrysogenum strains.
- Cephalosporins are a group of antibiotics of great therapeutic importance produced industrially by fermentation of A. chrysogenum or alternatively by chemical expansion of the penicillin thiazo-15 ring generating a dihydrotiazine ring.
- chrysogenum or alternatively by chemical expansion of the penicillin thiazo-15 ring generating a dihydrotiazine ring.
- side chains in different positions of the ⁇ -lactam and dihydrotiazine rings, a huge number of cephalosporins with greater antibiotic capacity or better pharmacokinetic properties can be synthesized.
- the preparation of these semi-synthetic antibiotics has
- 7-Aminodeacetoxycephalosporic acid (7-ADCA) is one of the substrates used for the preparation of cephalosporins characterized by the absence of functionalization at position 3. Due to this absence of functionalization, 7-ADCA is generally synthesized by expansion of the thiazolidine ring of penicillins obtained by fermentation, while the rest of the cephalosporic compounds are prepared from natural cephalosporins obtained by fermentation. Room
- 7-ADCA is produced from penicillin G or V, requiring 4 to 5 chemical stages to expand the 5-member ring.
- This process like many of the chemical processes, has a series of disadvantages compared to biological processes: it is a complex process with multiple stages, it requires the use of expensive and toxic chemical reagents, it uses organic solvents harmful to the environment and during the process originate by-products and impurities that require complex purification systems.
- different strategies for obtaining 7- ADCA based on the fermentation of microorganisms and bioconversion have been described.
- DAO D-amino acid oxidase
- glutaryl-7-ACA acylase which hydrolyzes the glutaric acid side chain generating 7-ACA
- 6-APA produced directly by fermentation and / or enzymatic hydrolysis of penicillins G or V could be expanded to 7- AC ⁇ through the use of DAOCS, but unfortunately none of the DAOCS described so far recognize 6- APA as a substrate and therefore are unable to effect the expansion of the thiazolidine ring.
- the possibility of expanding G or V penicillins, both in vitro and in vivo has been described by means of a DAOCS to form the corresponding G or V cephalosporins, which can be enzymatically hydrolyzed to 7-ADCA.
- the efficiencies and productivity of these systems are far from an industrial application.
- adipoil-7-ADCA has been described through the use of a recombinant strain of P. chrysogenum in which the DAOCS of S. clavuligerus has been expressed.
- the addition of adipic acid during fermentation causes the production of adipoil-6-APA, a molecule that is recognized in vivo by DAOCS causing the expansion of the thiazolidine ring and producing adipoyl-7-ADCA.
- the adipoyl-7-ADCA is purified from the rest of the components of the fermentation medium (in particular the adipoyl-6-APA) and enzymatically hydrolyzed by the use of an adipyl acylase generating 7-ADCA.
- ⁇ -Lactam antibiotics are derived from three precursor amino acids: L- ⁇ -aminoadipic acid, L-cysteine and L-valine, which are condensed to form the precursor tripeptide ⁇ - (L- ⁇ -aminoadipil) -L-cysteinyl-D -valine (LLD-ACV) by the enzyme ACV synthetase (ACVS).
- IPNS isopenicillin N synthase
- IPN isopenicillin N
- This compound has a weak antibiotic activity and is the precursor of both penicillins and cephalosporins and cephamycins.
- the transformation of IPN into penicillin G takes place by exchanging the side chain of ⁇ -aminoadipic acid for another of phenylacetic acid, previously activated as phenylacetyl-CoA, in a reaction catalyzed by the enzyme acyl-CoA: 6-APA acyltransferase.
- the following reaction consists in the hydroxylation of the DAOC molecule to generate deacetylcephalosporin C (DAC) and is catalyzed by the enzyme DAC synthetase (DACS) or hydroxylase.
- DAC deacetylcephalosporin C
- CPC cephalosporin C
- pcbAB codes for ACVS (Gutiérrez et al. 1991. J. Bacteriol. 173: 2354-2365), pcbC codes for IPNS (Samson et al. 1985. Nature 318: 191-194), cefEF codes for DAOCS / DACS (Samson et al. 1987. Biotechnology 5: 1207-1214) and cefG codes for DAC acetyltransferase (Gutierrez et al. 1992. J. Bacteriol. 174: 3056-3064).
- DAOCS and DACS activities are located in a single polypeptide (Scheidegger et al. 1984. J. Antibiot. 37: 522-531) encoded by the cefEF gene in A. chrysogenum (Samson 1987. Biotechnology 5: 1207-1214), while in the microorganisms pro Cephamycin ductors, these activities are located in two independent polypeptides encoded respectively by the cefE genes (Kovacevic et al. 1989. J. Bacteriol. 171: 754-760; Barredo et al. 1991. Microbiology 7: 1-12; Ingolia TD et al. 1991. US5070020; Coque et al. 1993. Mol Gen. Genet.
- chrysogenum capable of producing penicillin G have been developed by heterologous expression of the penDE gene of Penicillium chrysogenum (Gutiérrez et al. 1990. Mol. Gen. Genet. 225: 56-64).
- the penDE gene codes for the acyl-CoA enzyme activity: 6-APA acyltransferase, which is capable of exchanging the side chain of ⁇ -aminoadipic acid present in the isopenicillin N molecule for another consisting of aromatic acids, such as phenylacetic acid .
- Another type of transfor- A. chrysogenum mantés of great interest are those capable of directly producing 7-amino cephalosporic acid (7- AC ⁇ ) (Isogai et al. 1991.
- A. chrysogenum was transformed with the genes that code for the D-amino acid oxidase (DAO) activities of Fusarium solani and glutaryl-7-ACA acylase (GLA) of Pseudo-monas diminuta.
- DAO D-amino acid oxidase
- GLA glutaryl-7-ACA acylase
- 7-ADCA is used as a substrate for the production of semisynthetic cephalosporins.
- the most commonly used industrial process for the production of 7-ADCA consists in the expansion of the thiazolidine ring of penicillin by chemical methods.
- the starting point for the chemical synthesis of 7-ADCA is an aqueous solution of penicillin, which is oxidized to penicillin sulfoxide with a peroxide and then its ring of 5 carbon atoms expands forming a ring of 6 atoms (acid 7 -phenylacetamide deacetoxycephalosporic acid or FADCH), in the presence of an appropriate chemical catalyst.
- the purified FADCH is subjected to an enzymatic treatment catalyzed by the enzyme penicillin amidase or penicillin acylase for the elimination of the amide bond present in its molecule.
- reaction products 7-ADCA and the side chain are obtained.
- the last step is the purification of 7-ADCA.
- A. chrysogenum expresses the DAOCS enzyme capable of expanding the thiazolidine ring of penicillins to the dihydrotiazine ring of cephalosporins.
- the cefEF gene is present, which codes for the bifunctional enzyme DAOCS / DACS. Therefore, the final product of this enzyme is DAC and not DAOC as occurs in cephamycin producing organisms, which have separate DAOCS and DACS enzymes.
- Several types of penicillins have been treated with an acellular extract of A. chrysogenum obtaining the corresponding cephalosporins (Demain et al. 1979. US4178210; Demain et al. 1979.
- penicillin G an intermediate compound of the biosynthesis of cephalosporins and cephamycins not commercially available
- penicillins produced industrially by P. chrysogenum penicillin G and penicillin V.
- the idea of modifying The substrate specificity of the DAOCS enzyme in order to be able to use penicillin G as a substrate and expand it to phenylacetyl-7-ADCA has been shared by a number of research groups for years (Cantwell et al. 1990. Curr. Genet. 17: 213-221), however, its achievement has not yet been described.
- chrysogenum is an efficient substrate for DAOCS since its side chain (adipic acid) is equivalent to that of penicillin N, the natural substrate of DAOCS.
- adipic acid side chain
- penicillin N the natural substrate of DAOCS.
- 5 rum transformants were selected from industrial strains of P. chrysogenum expressing the S. clavuligerus cefE gene, which were capable of producing adipil-7- ADCA in a proportion of 17% with respect to the production of penicillin V of the parental strain.
- adipic acid side chain was efficiently removed by treatment with the Pseudomonas cephalosporin acylase activity or enzyme.
- P. chrysogenum transformants expressing the cefEF gene (DAOCS- DACS) from C. acremonium produced both adipyl-7-ADCA and adipyl-7-aminodeacetylcephalosporic acid (adipil-7-ADACA).
- P. Chrysogenum transformants expressing the cefEF and cefG genes (DAC acetyltransferase) produced a mixture of adipyl-7-ADCA, adipil-7-ADACA and adipil-7-ACA.
- the method included in the present patent consists in obtaining a strain of A. acremonium whose biosynthetic route of CPC is blocked at the level of the DAOCS / DACS enzymatic activity, so that it produces penicillin N.
- This strain is obtained by inactivating the cefEF gene function by gene disruption by a double recombination process with a plasmid in which the cefEF gene is disrupted. Inactivation of gene function by disruption is a technique used for the modification of biosynthetic pathways and the accumulation of biosynthetic intermediates useful in obtaining certain secondary metabolites.
- this technique has been used successfully in industrial strains of filamentous fungi. As an example in P.
- chrysogenum the disruption of the lys2 gene can be cited, making lysine auxotrophic strains capable of biosynthesizing a greater amount of ⁇ -aminoadipic acid, which intervenes as a precursor to penicillin biosynthesis (Casqueiro et al., 1997. Spanish
- chrysogenum the inactivation of the pcbAB genes (Hoskins et al. 1990. Curr. Genet. 18: 523-530), pcbC (Waltz and Kück. 1993. Curr. Genet. 24: 421-427) and cefG (Matsuda et al. 1992. Biochemistry. Biophys. Res. Commun. 186: 40-6), obtaining in all cases transformants incapable of producing cephalosporin C.
- A. chrysogenum is a species where it is not they obtain gene disruptions with ease (Waltz and Kück. 1993. Curr. Genet. 24: 421-427), in this patent the inactivation by cefEF gene disruption is described for the first time. The strain of A.
- ketoadipil-7-ADCA formed reacts non-enzymatically with hydrogen peroxide to give rise to 7- ⁇ - (carboxybutanamide) -deacetoxycephalosporin (glutaryl-7-ADCA or GL-7- ADCA).
- glutaryl moiety of GL-7-ADCA is removed by treatment with the GLA enzyme generating 7-ADCA.
- the described process allows obtaining 7-ADCA by a completely biological route and without the use of organic solvents.
- DAOC as an intermediate useful in the production of 7-ADCA has not been described so far due to the impossibility of obtaining DAOC as the sole product of the fermentation of A. chrysogenum. In all cases a mixture of DAOC and DAC difficult to separate subsequently occurs. Also, the difficulty of eliminating until recently the aminoadipic acid side chain of the DAOC molecule using enzymatic procedures made the DAOC a molecule not suitable for the industrial manufacture of 7-ADCA. However, the construction of the strain A. chrysogenum ⁇ cefEF-cefE, allows a high production of DAOC, the latter being the majority cephalosporin.
- the DAOC produced can be transformed to 7-ADCA by using the DAO and GLA enzymes by methods equivalent to those previously described (Croux et al. 1994. US5354667; Cambiaghi et al. 1991. EP0496993; Garc ⁇ a et al. 1998. EP0843015 ).
- EXAMPLE 1 CefEF gene disruption in A. chrysogenum.
- the hygromycin resistance cassette formed by the promoter of the gpd (glyceraldehyde-3-phosphate dehydrogenase) gene of A. Nidulans, the hygromycin resistance gene (hyg R ) and the terminator was integrated of the trpC gene (tryptophan C), at the Xhol site of the cefEF gene ( Figure 1A-B). To do this, the Xhol site present in pBC KS (+) was first eliminated by double Sall-Xhol digestion and religation, resulting in the pBC * KS (+) plasmid which lacks the Xhol and Sal ⁇ restriction sites.
- gpd glyceraldehyde-3-phosphate dehydrogenase
- chrysogenum containing the cefEF and cefG genes respectively The transformants of A. chrysogenum with pALC73 were obtained according to previously described procedures (Queener et al. 1985. Lieve (ed.) Microbiology-1985. Pp. 468-472. ASM. Washington) and were selected for resistance to 5-10 ⁇ g / ml hygromycin.
- the strain that includes plasmid pALC73, called E. coli TOP10 / pALC73 was deposited in the Spanish Type Culture Collection (CECT), University of Valencia, Research Building, Burjasot Campus, 46100 Burjasot (Valencia) with No. 5151 .
- the transformants selected for hygromycin resistance were grown on LPE solid medium plates (Le Page & Campbell, 1946, J. Biol. Chem. 162: 163-171) by incubation for 7 days at 28 ° C. Then two cylinders or dowels were removed from each transformant with the help of a punch.
- one of the tacos was bioassayed on a LB medium plate to which a culture of E. coli sensitive to ⁇ -lactams (E. coli ESS) and a penicillinase type preparation were added. I (Sigma P-0389) at a final concentration of 0.1 mg ml. The other taco was tested on a LB medium plate with E.
- transformants 2672, 2673, 2675, 2680, 2691, 2692, 2693, 2694, 2695, 2696, 2697, 2698 and 2699 (marked with an asterisk) possessed the characteristics sought and were selected for subsequent tests.
- EXAMPLE 2 Production of penicillin N by A. chrysogenum ⁇ cefEF.
- Fermentation in liquid medium was initiated by inoculating the spores from a slant in a 250 ml flask containing 50 ml of medium or complex inoculum (Shen et al, 1986, Bio / Technology 4: 61-64). East flask was incubated for 48 hours at 28 ° C and 250 rpm to obtain an abundant vegetative mycelium. Fermentation under antibiotic production conditions began by inoculating 0.5 ml (3%) of the previous vegetative culture in a 500 ml flask with 15 ml of fermentation complex medium (Shen et al., 1986, Bio / Technology 4: 61-64).
- This culture was incubated for 7 days at 25 ° C and 250 rpm, collecting samples of 1 ml every 24 hours for the study of antibiotic production throughout the fermentation. Subsequently, analyzes were carried out in order to determine: (I) that the antibiotic produced by the A. chrysogenum ⁇ cefEF transformants was penicillin N, (II) the level of penicillin N production of these transformants and (III) the eventual presence of others antibiotics in the fermentation medium.
- the fermentation samples were centrifuged for 5 minutes at 20,000 g to remove the mycelium and then 50 ⁇ l of the supernatant was diluted 10 times with water and analyzed on a Waters TAD chromatograph (Waters Associates, Milford, MA) with 486M1 detector, pump W600, auto injector 717 and a Nucleosil C 18 -10 ⁇ m column (250x4.6 mm) (Phenomenex, Torrance, CA).
- the samples were analyzed with a flow of 2.3 ml / min and a variable wavelength (214 to detect penicillins or 254 nm to detect cephalosporins).
- Enzymatic expansion of penicillin N was performed using resting cells or cell-free extracts of Streptomyces clavuligerus NP1. This strain practically does not produce cefamycin when compared to the parental strain (Mahro and Demain, 1987), which facilitates the determination of cephalosporins and / or cephamycins synthesized in vitro from penicillin N.
- MST inoculum medium 1% soluble starch, 3% trypticase I am broth without dextrose, 90 mM MOPS; pH 7.0
- MST was also used as fermentation medium, in this case inoculating with 5% of the previous culture and incubating at 200 rpm and 28 ° C for 24 hours.
- the cells were collected by centrifugation (10,000 rpm / 10 min. / 4 ° C) and were they washed twice with demineralized water. Finally, the cells were resuspended in 40 ml of demineralized water.
- the reaction mixture (10 ml) for enzymatic expansion was prepared according to the procedures described (Shen et al. 1984. Enzyme Microbiol. Technol. 6: 402-404) using penicillin N produced by A. chrysogenum ⁇ cefEF TI as substrate : 50 mM MOPS pH 6.5, FeS0 4 (heptahydrate) 1.8 mM, 2.56 mM ⁇ -ketoglutaric acid, 8 ml of cell suspension and 0.2 mM N penicillin.
- the reaction was incubated at 30 ° C and 200 rpm for 3 hours. At different times, 1 ml samples were taken, which were centrifuged at 13,000 rpm for 10 minutes. The supernatants obtained were analyzed by bioassay and HPLC.
- the bioassay was performed on LB plates (2% agar) containing E. coli ESS as indicator microorganism and 50,000 Ul / ml penicillinase (Difco Bacto penase concentrate, Difco Laboratories, Detroit, MI). In wells prepared with the aid of a punch, 200 ⁇ l of each sample was placed and the plates were incubated at 37 ° C for 16 hours. Samples taken at zero time did not show growth inhibition halos of E. coli ESS, while those incubated for 1 or 3 hours resulted in 17 and 21 mm inhibition halos respectively, indicating the presence of reaction products (cephalosporins ) resistant to penicillinase.
- HPLC analysis was performed with a Waters TAD device (Waters Associates, Milford, MA) with 486M1 detector, W600 pump, 717 autoinjector and a Nucleosil C 18 -10 ⁇ m column (250x4.6 mm) (Phenomenex, Torrance, CA) .
- the samples of the expansion reactions (50 ⁇ l) were analyzed with a flow of 2.3 ml / min and a variable wavelength (214 or 254 nm). The analysis at 214 nm serves to determine the presence of penicillins and at 254 nm to detect cephalosporins.
- Elution was performed using as a mobile phase ammonium formate pH 3,3-methanol-tetrahydrofuran (99.85: 0.1: 0.05) in isocratic mode for 30 minutes.
- the 214 nm analysis of the fermentation broth used as a substrate revealed the existence of a peak at 10.1 minutes that coincides with the retention time obtained for penicillin N, while at 254 nm it did not reveal the existence of cephalosporins.
- the MST inoculum medium (1% soluble starch, 3% trypticase I'm broth without dextrose, 90 mM MOPS; pH 7.0) was seeded with a spore suspension of S. clavuligerus NP1 and incubated at 200 rpm. and 28 ° C for 48 hours. Cells were collected by centrifugation (10,000 rpm JIO min J4 ° C), washed twice with buffer E (15 mM Tris HCl pH 7.5, 10% ethanol) and resuspended in buffer E containing 10 mM DTT and 2 mM PMSF . The cells were broken by sonication using a Labsonic 2000 (Braun Biotech) applying 3 pulses of 25 seconds. The sample was centrifuged at 20,000 rpm. and 4 ° C for 20 minutes. The resulting extract was divided into aliquots that were frozen at -20 ° C and their protein content was analyzed by Bradford's technique.
- the reaction for enzymatic expansion was carried out by adding
- plasmid pALE36 ( Figure 4A-B) was constructed, which has a 400 bp BglII-Clal fragment of plasmid pT7-7 (Tabor and Richardson, 1985, Proc. Nati. Acad. Sci USA 82: 1074-1078) in pBC KS +. This fragment contains a multiple cloning region downstream of the ⁇ 10 bacteriophage T7 gene promoter.
- pALE36 has the advantage over pT7-7 of using the Cm R gene instead of the Ap R gene. This last gene codes for a ⁇ -lactamase activity that would degrade the penicillin N used as a substrate.
- pALE36 was digested Ndel-BamHI and a 1.25 Kb Ndel-BglII fragment containing the cefE of N. lactamdurans. Finally, it was verified by sequencing that the fusion between the promoter and the cefE gene was correct and with the plasmid obtained, pALE38, competent cells of E. coli / JM109 (DE3) were transformed, selecting the transforming clones for chloramphenicol resistance .
- IPTG was added to a final concentration of 1 mM and incubated for a further 6 hours.
- the processing of the cells obtained, the enzymatic expansion reaction and the bioassay and HPLC analysis of the reaction products were carried out as indicated in section 2.3.2.
- EXAMPLE 3 Expression of the cefE gene in A. chrysogenum ⁇ cefEF.
- the cefE gene which codes for DAOCS activity, was expressed in A. chrysogenum ⁇ cefEF.
- actinomycetes S. clavuligerus, Nocardia lactamdurans and Lysobacter lactamgenus.
- A. chrysogenum ⁇ cefEF a series of plasmids were designed in which the cefE genes of S. clavuligerus and N. lactamdurans were placed under the control of fungal gene promoters.
- Two promoters were chosen: one corresponding to a gene with high level of expression under fermentation conditions (pcbC gene, which codes for IPNS in P. chrysogenum) and the other belonging to the gpdA gene of A. nidulans, which constitutively expresses and codes for glyceraldehyde 3-phosphate dehydrogenase activity.
- the set formed by the fungic promoter, the cefE gene and the transcription terminator of the trpC gene of Aspergillus niger was inserted into plasmid pALfleo7 (Barredo et al. 1997. Spanish patent P9700482).
- This 5.4 kb plasmid has the phleomycin resistance gene of Streptoalloteichus hindustanus flanked by the promoter of the gdhA gene of P. chrysogenum and the transcription terminator of the trpC gene of Aspergillus niger.
- the S. clavuligerus cefE gene was subcloned into plasmid pBluescript I KS (+) (Stratagene) as a 1.6 kb BamHI-Kpnl fragment of the S. clavuligerus genome that includes the complete cefE gene and 582 bp of end 3 'of the cefD gene.
- an Ncol restriction site was created at the 5 'end of said gene by directed mutation using the commercial kit QuikChange (Stratagene) and the oligonucleotides described in SEQ ID No. 1 and SEQ ID No. 2.
- the mutation process was carried out following the manufacturer's instructions and the creation of the Ncol restriction site and the absence of additional mutations were verified by sequencing.
- the plasmid resulting from this process was called pALC83 ( Figure 6A-B).
- pALC83 was digested with Kpnl, its ends were filled with Klenow, it was subsequently digested with Ncol and finally the 0.95 kb DNA fragment was purified using the QIAEX II kit (Qiagen). The 0.95 kb fragment was then ligated with the plasmid pALpcbC ( Figure 6A-B) digested with HindIII, filled in with Klenow and digested with Ncol.
- pALC87 contains: (I) the S. clavuligerus cefE gene expressed under the control of the penicillium chrysogenum pcbC gene promoter and (II) the A. nidulans trpC gene downstream of the cefE gene .
- pALC87 was digested with PvuII and the fragment containing the cefE gene was ligated with pALfleo7 digested with EcoRV and dephosphorylated.
- plasmid pALC88 was obtained, which is a carrier of the PpcbC-cefE-TtrpC insert ( Figure 6A-B).
- the S. clavuligerus cefE gene was also expressed under the promoter of the gpdA gene of A. nidulans.
- the 0.95 kb fragment containing the S. clavuligerus cefE gene used in the construction of pALC87 was ligated with the plasmid pTh (Uriach et al. 1995. EPA 0684312A2) digested with HindIII, filled with Klenow and later digested with Ncol.
- the S. clavuligerus cefE gene is located under the control of the gpdA gene promoter of A.
- the cefE gene of N. lactamdurans was obtained by digestion of pALE38 (section 2.3.3) with Ndel, filled in with Klenow and digestion with HindIII. In this way, a 1,250 bp DNA fragment that was ligated with the plasmid pALpcbC ( Figure 6A-B) previously digested with Ncol was purified, the protruding ends were removed with Mung Bean nuclease and digested with HindIIL in the resulting plasmid (pALC84 ) the cefE gene of N. lactamdurans is under the control of the pcbC gene promoter of Penicillium chrysogenum and followed by the trpC gene terminator of A.
- plasmids pALC85 and pALC86 were obtained in which the PpcbC-cefE-TtrpC insert is in the two possible orientations.
- the strain that includes plasmid pALC85, called E. coli DH5 ⁇ / pALC85 was deposited in the Spanish Type Culture Collection (CECT), University of Valencia, Research Building, Burjasot Campus, 46100 Burjasot (Valencia) with No. 5150.
- the cefE gene of N. lactamdurans was also expressed under the promoter of the gpdA gene of A. nidulans.
- the 1,250 bp 5 DNA fragment containing the N. lactamdurans cefE gene described above was ligated with the plasmid pTh (Uriach et al. 1995. EPA 0684312A2) digested with Ncol, the protruding ends with Mung Bean nuclease removed and later digested with HindIII.
- the cefE gene of N. Lactamdurans is under the control or promoter of the gpdA gene of A.
- the selected transformants were grown on LPE medium or solid plates (Le Page & Campbell, 1946, J. Biol. Chem. 162: 163-171) by incubation for 7 days at 28 ° C. Then, with the help of a punch, a cylinder or block was extracted from each transformant, which was bioassayed on a plate of LB medium to which a culture of E. coli sensitive to ⁇ -lactams (E. coli) was added ESS) and a penicillinase type I (Sigma) preparation at a final concentration of 0.1 mg / ml. In this way, those transformants that produced the greatest halos of inhibition were selected, indicating that they were able to synthesize larger amounts of penicillinase resistant antibiotics.
- Total DNA was digested with the enzymes BamHI and Sal ⁇ and, after separation by electrophoresis and transfer to nitrocellulose filters, hybridized using a 0.6 kb HindlII-Kpnl probe corresponding to the cefE gene of N. lactamdurans (transformants 2800 0 2808) or with a 0.5 kb Kpnl-Scal probe corresponding to the S. clavuligerus cefE gene (transformants 2809 to 2817).
- the result obtained showed the expected restriction pattern: the parental strain A. chrysogenum and the strains A. chrysogenum ⁇ cefEF TI, A. chrysogenum ⁇ cefEF T2 and A.
- chrysogenum ⁇ cefEF T3 did not show any hybridization band, while in the transformants called A.
- chrysogenum ⁇ cefEF-cefE appeared additional hybridization bands indicating the integration of the cefE gene in the genome of A. chrysogenum ⁇ cefEF.
- the fermentation of the strains of A. chrysogenum, A. chrysogenum ⁇ cefEF and A. chrysogenum ⁇ cefEF-cefE was performed as indicated in section 2.1, determining (I) that the antibiotic produced by A. chryso-genum ⁇ cefEF-cefE DAOC was, (II) the level of DAOC production of 5 such transforming strains and (III) the possible presence of other antibiotics in the fermentation medium.
- a total of 10 liters of the A. chrysogenum ⁇ cefEF-cefE fermentation broth containing 20 g / 1 DAOC was adjusted to pH 3.0 with concentrated H 2 S0 4 and filtered through a vacuum Büchner filter and with the help of a diatomaceous earth as a filter aid.
- the filtered biomass is washed with 10 liters of demineralized water in order to deplete the entire DAOC of the biomass, obtaining a volume of filtered broth of 16.4 liters.
- the filtered broth was passed through a column (1 mx 16 cm) of XAD4 polystyrene resin (Rohm & Haas) packed with 10 liters of resin at a flow rate of 150 ml / min.
- the column was washed with approximately 10 liters of water and eluted with a 0.15 M solution of sodium acetate.
- the eluted fractions were analyzed by HPLC to examine the DAOC content. Subsequently, those fractions rich in DAOC were collected, obtaining an eluate volume of 23.5 liters.
- the eluate was adjusted to pH 5.5 and subjected to decolorization and purification by percolation through a column packed with IRA68 ammonium exchange resin (Rohm & Haas).
- the decolorized solution was subjected to a process of concentration by reverse osmosis until a concentration greater than 200 g / 1 of DAOC was reached.
- DAOC bioconversion in glutaryl-7-ADCA catalyzed by immobilized DAO To perform the bioconversion of the DAOC obtained as described in example 4, 66 g of DAOC sodium salt was dissolved in 2 liters of 25 mM potassium phosphate buffer containing 0.5 g of sodium metabisulfite. The DAOC solution was placed in a 3 liter reactor containing 150 g of DAO immobilized in Eupergit C (Rohm) according to the methods described (Cambiaghi et al. 1991. EP 496993). The mixture was incubated at 25 ° C with slight stirring and bubbling of oxygen (1 vol./volJmin.) Through a diffuser located at the bottom of the reactor. The pH was maintained at 7.5 by automatic addition of 5% ammonium hydroxide. In 75 minutes, the DAOC was completely transformed to obtain a mixture of glutaryl-7-ADCA (90%) and ketoadipil-7-ADCA (10%).
- ketoadipil-7-ADCA To transform the residual ketoadipil-7-ADCA to glutaryl-7-ADCA, the solution obtained after incubation was filtered off the immobilized enzyme and 10 ml of hydrogen peroxide (H 2 0 2 ) was added at a concentration of 3 , 5% per liter of filtrate. The mixture was incubated for 15 minutes at 25 ° C, after which 0.5 g of sodium pyruvate was added. After said treatment the ketoadipil-7-ADCA is completely transformed into glutaryl-7-ADCA, obtaining a solution of 3% glutaryl-7-ADCA. Said solution was concentrated by osmosis at 4 ° C to a concentration of 5% to be used in the next enzymatic stage.
- H 2 0 2 hydrogen peroxide
- the solids were then separated from the fermentation, mainly containing A. Chrysogenum and Rhodotorula gracilis cells together with varying amounts of insoluble material from the fermentation medium, by using an ultrafiltration system through membranes with a molecular cut of 30,000 Da.
- ketoadipil-7- ADCA was transformed into glutaryl-7-ADCA, obtaining a solution with a richness in glutaryl-7-ADCA of 1%. Said solution was concentrated by reverse osmosis to an approximate concentration of 5% in glutaryl-7-ADCA.
- the organic phase was washed with 0.25 liters of 25 mM phosphate buffer at pH 8.0 in order to exhaust it thoroughly from glutaryl-7-ADCA.
- Aqueous extracts obtained containing 50 g / 1 glutaryl-7-ADCA were pooled and used directly for the next enzymatic conversion or were subjected to a vacuum distillation process to remove organic solvent residues and thus increase the life of the catalyst in the next enzymatic conversion.
- 7-ADCA was treated with 3 g of active carbon for 20 minutes at room temperature and with stirring in order to remove impurities and reduce the color of the final product.
- the coal was removed by filtration and washed with 10 ml of water.
- the filtered solution was adjusted to pH 4.5 by the addition of H 2 S0 4 6 N, so that crystallization of 7-ADCA was initiated, and it was incubated for 15 minutes with slow stirring so that crystallization progressed.
- the temperature was lowered to 5-10 ° C, the pH finally being adjusted to 3.8 by the addition of H 2 S0 4 6 N. After 2 hours of incubation, the precipitate was recovered by filtration and was washed with 25 ml of water.
- the crystallization yield was 95.5%, obtaining a 7-ADCA with a purity of 98.3%.
- the coal was removed by filtration and washed with 10 ml of water.
- the filtered solution was adjusted to pH 3.0 by the addition of 2N ammoniacal solution so that crystallization of 7-ADCA was initiated. It was then incubated for 15 minutes with slow stirring until crystallization progressed, finally adjusting the pH to 3.7 by adding NH 4 OH 2N and at a temperature between 5-10 ° C. After 2 hours the precipitate was recovered by filtration and washed with 25 ml of water.
- the crystallization yield was 94%, obtaining a 7-ADCA with a purity of 98.5%.
- EXAMPLE 7 Production of 7- ADCA by A. chrysogenum ⁇ cefEF-cefE-dao-gla and A. chrysogenum ⁇ cefEF-cefE-cac. Based on a previous description, it is possible to construct a biosynthetic operon for the production of 7-ACA directly by fermentation of A. chrysogenum (Isogai et al. 1991 Biotechnology 9: 188-91). The system described is based on the expression in A. chrysogenum of the genes (cDNAs) that code for the DAO and GLA enzymatic activities of Fusarium solani and Pseudomonas diminuta respectively. Applying this same idea to the strain A.
- cDNAs genes
- chrysogenum ⁇ cefEF-cefE it is possible to construct a biosynthetic operon for the production of 7- ADCA directly by fermentation of A. chrysogenum ⁇ cefEF-cefE-dao-gla. In this case, due to the impossibility of A. chrysogenum ⁇ cefEF-cefE to produce CPC, the biosynthesis of 7-ADCA takes place instead of 7-ACA.
- the 7-ADCA produced by fermentation of A. chrysogenum ⁇ cefEF-cefE-dao-gla can be purified as indicated in example 6.
- FIGURES Figure 1A-B DESCRIPTION OF THE FIGURES Figure 1A-B.- Scheme of the construction of pALC73, plasmid for the disruption of the cefEF gene of A. chrysogenum.
- plasmid pExp7.2 BB which includes the cefEF and cefG genes of A. chrysogenum, was subcloned into the BamHI site of plasmid pBC KS (+) which had previously been inactivated the Xhol site.
- the resulting plasmid was named pALC72.
- the hygromycin resistance (hygR) gene was then inserted into the Xhol site of the cefEF gene giving rise to pALC73.
- This 14.6 kb plasmid includes the chloramphenicol resistance gene (CmR) as a selection marker in E. coli and the hygromycin resistance gene (hygR) as a selective marker in A. chrysogenum.
- CmR chloramphenicol resistance gene
- hygR hygromycin resistance gene
- the hygR gene is expressed under the control of the gpd promoter (glyceraldehyde 3 phosphate dehydrogenase) and has the trpC terminator (tryptophan C).
- FIG. 2 Scheme of the disruption of the cefEF gene of A. chrysogenum. Plasmid pALC73 is integrated by double recombination in one of the A. chrysogenum chromosomes inactivating the cefEF gene. In the lower part of the figure, Southern analysis of the disrupted A. chrysogenum clones in the cefEF gene is shown. The hybridization band obtained by BamHI digestion of the parental strain DNA is converted into two 5.7 kb and 5.3 kb bands in the transforming strains with the inactivated cefEF gene. Similarly, the 0.5 kb hybridization band I left the parental strain becomes another 2.2 kb band in the disrupted transformants in the cefEF gene.
- the disrupted transformants are called A. chrysogenum ⁇ cefEF TI, A. chrysogenum ⁇ cefEF T2 and A. chrysogenum ⁇ ce EF T3.
- P corresponds to the parental strain A. chrysogenum
- TI, T2 and T3 are transformants of A. chrysogenum with the inactivated cefEF gene.
- Figure 3 Enzymatic expansion of penicillin N to DAOC using enzymatic extracts free of S. clavuligerus NP1 cells.
- a chromatogram of the reaction mixture is shown at time 0 and in the lower panel the same mixture after 2 hours of reaction.
- Abcisas time in minutes; Ordered: Optical Density (D.O.)
- FIG. 4A-B Scheme of the construction of pALE38, plasmid for the expression of the cecard gene of Nocardia lactamdurans in E. coli under the control of T7 promoter.
- FIG. 5A-B Analysis by LC masses of penicillin N produced by A. chrysogenum ⁇ cefEF TI. In the upper panel a chromatogram is shown at 214 nm and in the lower one the mass spectrum corresponding to the peak eluting at 11.92 minutes.
- Figure 6A-B Scheme of the construction of pALC88, plasmid for the expression of the S. clavuligerus cefE gene in A. chrysogenum under the control of the pcbC gene promoter of P. chrysogenum.
- Figure 7A-B Scheme of the construction of pALC93 and pALC94, plasmids for the expression of the S. clavuligerus cefE gene in A. chrysogenum under the control of the gpdA promoter of A. nidulans.
- Figure 8A-B Scheme of the construction of pALC85 and pALC86, plasmids for the expression of the cefE gene of N. lactamdurans in A. chrysogenum under the control of the pcbC gene promoter of P. chrysogenum.
- Figure 9A-B Scheme of the construction of pALC90 and pALC91, plasmids for the expression of the cefE gene of N. lactamdurans in A. chrysogenum under the control of the gpdA gene promoter of A. nidulans.
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AU52231/00A AU5223100A (en) | 1999-06-18 | 2000-06-13 | Method for producing 7-aminodesacetoxycephalosporanic acid (7-adca) |
KR1020017016020A KR20020022701A (ko) | 1999-06-18 | 2000-06-13 | 7-아미노데스아세톡시세팔로스포란산의 제조 방법 |
EP00936911A EP1188838A1 (en) | 1999-06-18 | 2000-06-13 | Method for producing 7-aminodesacetoxycephalosporanic acid (7-adca) |
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ES009901365A ES2165276B1 (es) | 1999-06-18 | 1999-06-18 | Procedimiento biotecnologico para la produccion de acido 7-aminocefal osporanico (7-adca) y compuestos de sintesis intermedios particularmente penicilina n y desacetoxicefalosporina c (daoc) |
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WO2002085914A2 (en) * | 2001-04-19 | 2002-10-31 | Bioferma Murcia, S.A. | ENZYMATIC PROCESS FOR PREPARING CEPHALOSPORANIC ACID DERIVATIVES USING α-KETOACID DERIVATIVES |
CN103525796A (zh) * | 2013-10-18 | 2014-01-22 | 江苏辉腾生物医药科技有限公司 | 具有戊二酰基-7-氨基头孢烷酸酰化酶和d-氨基酸氧化酶活性的重组酶及其制备方法和应用 |
CN112852912A (zh) * | 2020-04-10 | 2021-05-28 | 中国科学院天津工业生物技术研究所 | 一种合成7-氨基去乙酰氧基头孢烷酸的方法 |
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EP2614066B1 (en) * | 2010-09-07 | 2017-11-29 | DSM Sinochem Pharmaceuticals Netherlands B.V. | Process for the production of cephalosporins |
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KR102194740B1 (ko) * | 2019-09-18 | 2020-12-23 | 아미코젠주식회사 | 7-adca 제조를 위한 데아세트옥시세팔로스포린 c의 고농도 생산 재조합 아크레모니움 크리소제눔 균주의 제조방법 및 이 방법으로 제조된 균주 |
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KR102688632B1 (ko) * | 2022-10-26 | 2024-07-26 | 아미코젠주식회사 | 7-adca 제조를 위한 데아세톡시세팔로스포린 c의 고농도 및 고순도 생산 균주 및 이를 이용한 제조 방법 |
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WO2002085914A2 (en) * | 2001-04-19 | 2002-10-31 | Bioferma Murcia, S.A. | ENZYMATIC PROCESS FOR PREPARING CEPHALOSPORANIC ACID DERIVATIVES USING α-KETOACID DERIVATIVES |
WO2002086143A2 (en) * | 2001-04-19 | 2002-10-31 | Bioferma Murcia, S.A. | Enzymatic process for preparing cephalosporin derivatives |
WO2002086143A3 (en) * | 2001-04-19 | 2003-01-09 | Bioferma Murcia S A | Enzymatic process for preparing cephalosporin derivatives |
WO2002085914A3 (en) * | 2001-04-19 | 2003-02-06 | Bioferma Murcia S A | ENZYMATIC PROCESS FOR PREPARING CEPHALOSPORANIC ACID DERIVATIVES USING α-KETOACID DERIVATIVES |
US6642020B2 (en) | 2001-04-19 | 2003-11-04 | Bioferma Murcia S.A. | Process for preparing cephalosporin derivatives |
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CN112852912A (zh) * | 2020-04-10 | 2021-05-28 | 中国科学院天津工业生物技术研究所 | 一种合成7-氨基去乙酰氧基头孢烷酸的方法 |
CN112852912B (zh) * | 2020-04-10 | 2022-01-11 | 中国科学院天津工业生物技术研究所 | 一种合成7-氨基去乙酰氧基头孢烷酸的方法 |
CN116656505A (zh) * | 2023-04-23 | 2023-08-29 | 四川大学 | 一种高产醇脱氢酶和酰基转移酶菌株及培养方法及应用 |
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