WO2000012704A3 - Expression fonctionnelle amelioree des recepteurs couples a la proteine g - Google Patents

Expression fonctionnelle amelioree des recepteurs couples a la proteine g Download PDF

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Publication number
WO2000012704A3
WO2000012704A3 PCT/US1999/020011 US9920011W WO0012704A3 WO 2000012704 A3 WO2000012704 A3 WO 2000012704A3 US 9920011 W US9920011 W US 9920011W WO 0012704 A3 WO0012704 A3 WO 0012704A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
coupled receptors
functional expression
enhanced functional
receptors
Prior art date
Application number
PCT/US1999/020011
Other languages
English (en)
Other versions
WO2000012704A9 (fr
WO2000012704A2 (fr
Inventor
Mark Henry Pausch
Margaret Lai
Sanford Silverman
Camelia Birsan
William Baumbauch
Eugene Tseng
Eileen Marie Kajkowski
Bradley Alton Ozenberger
Original Assignee
Mark Henry Pausch
Margaret Lai
Sanford Silverman
Camelia Birsan
William Baumbauch
Eugene Tseng
Eileen Marie Kajkowski
Bradley Alton Ozenberger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mark Henry Pausch, Margaret Lai, Sanford Silverman, Camelia Birsan, William Baumbauch, Eugene Tseng, Eileen Marie Kajkowski, Bradley Alton Ozenberger filed Critical Mark Henry Pausch
Priority to JP2000567691A priority Critical patent/JP2002523090A/ja
Priority to AU57009/99A priority patent/AU5700999A/en
Priority to CA002343046A priority patent/CA2343046A1/fr
Priority to EP99944033A priority patent/EP1112359A2/fr
Publication of WO2000012704A2 publication Critical patent/WO2000012704A2/fr
Publication of WO2000012704A9 publication Critical patent/WO2000012704A9/fr
Publication of WO2000012704A3 publication Critical patent/WO2000012704A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4722—G-proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Endocrinology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des récepteurs mutants couplés à la protéine G, lesquels exercent une action constitutive. L'invention concerne également des cellules de levure exprimant ces récepteurs, des vecteurs convenant à la fabrication de ces cellules et des méthodes de fabrication et d'utilisation associées.
PCT/US1999/020011 1998-09-01 1999-09-01 Expression fonctionnelle amelioree des recepteurs couples a la proteine g WO2000012704A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000567691A JP2002523090A (ja) 1998-09-01 1999-09-01 異種ｇタンパク質共役受容体の増強された機能的発現
AU57009/99A AU5700999A (en) 1998-09-01 1999-09-01 Enhanced functional expression of g protein-coupled receptors
CA002343046A CA2343046A1 (fr) 1998-09-01 1999-09-01 Expression fonctionnelle amelioree des recepteurs couples a la proteine g
EP99944033A EP1112359A2 (fr) 1998-09-01 1999-09-01 Expression fonctionnelle amelioree des recepteurs couples a la proteine g

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9870498P 1998-09-01 1998-09-01
US60/098,704 1998-09-01

Publications (3)

Publication Number Publication Date
WO2000012704A2 WO2000012704A2 (fr) 2000-03-09
WO2000012704A9 WO2000012704A9 (fr) 2000-06-08
WO2000012704A3 true WO2000012704A3 (fr) 2000-11-30

Family

ID=22270543

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US1999/020013 WO2000012705A2 (fr) 1998-09-01 1999-09-01 Methodes permettant d'ameliorer la fonction des recepteurs heterologues couples a la proteine g
PCT/US1999/020011 WO2000012704A2 (fr) 1998-09-01 1999-09-01 Expression fonctionnelle amelioree des recepteurs couples a la proteine g

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US1999/020013 WO2000012705A2 (fr) 1998-09-01 1999-09-01 Methodes permettant d'ameliorer la fonction des recepteurs heterologues couples a la proteine g

Country Status (5)

Country Link
EP (2) EP1112359A2 (fr)
JP (2) JP2002523090A (fr)
AU (2) AU756244B2 (fr)
CA (2) CA2344591A1 (fr)
WO (2) WO2000012705A2 (fr)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6528271B1 (en) 1997-06-05 2003-03-04 Duke University Inhibition of βarrestin mediated effects prolongs and potentiates opioid receptor-mediated analgesia
AU5561301A (en) * 2000-04-24 2001-11-20 American Home Prod Transgenic animal
US20040132107A1 (en) * 2000-10-26 2004-07-08 Grigliatti Thomas Anthony Functional assay for g-protein-coupled receptors based on insect cells
ATE357457T1 (de) * 2000-11-01 2007-04-15 Astellas Pharma Inc Verfahren zum screenen eines plättchenhemmers
US7018812B2 (en) 2000-11-03 2006-03-28 Duke University Modified G-protein coupled receptors
US7163800B2 (en) 2000-11-03 2007-01-16 Molecular Devices Corporation Methods of screening compositions for G protein-coupled receptor desensitization inhibitory activity
DE10056899A1 (de) 2000-11-16 2002-05-29 Aventis Pharma Gmbh Promotor zur funktionellen Charakterisierung von G-Protein gekoppelten Rezeptoren in der Hefe Saccharomyces cerevisiae
WO2002045503A2 (fr) * 2000-12-06 2002-06-13 Deltagen, Inc. Souris transgeniques contenant des disruptions geniques ciblees
GB0030038D0 (en) * 2000-12-08 2001-01-24 Univ Warwick Yeast-based assay
US7279324B2 (en) 2001-01-23 2007-10-09 Duke University Nucleic acid encoding G-protein coupled receptor with modified DRY motif
EP1336655A1 (fr) * 2002-01-12 2003-08-20 Aventis Pharma Deutschland GmbH Méthode pour l'identification des mutants ayant une actività constitutive
DE10233516A1 (de) * 2002-07-23 2004-02-12 Aventis Pharma Deutschland Gmbh Verfahren zur Identifizierung von Substanzen
WO2006104254A1 (fr) * 2005-03-29 2006-10-05 Bio-Energy Corporation Procede pour le criblage d'une substance de liaison des recepteurs
AU2006285393A1 (en) 2005-04-27 2007-03-08 Arena Pharmaceuticals, Inc. Combination therapy for the treatment of obesity and diabetes and conditions related thereto and for the treatment of conditions ameliorated by increasing a blood GLP-1 level
CA2613235A1 (fr) 2005-06-30 2007-01-11 Prosidion Limited Agonistes de gpcr
KR101281962B1 (ko) 2006-04-11 2013-07-08 아레나 파마슈티칼스, 인크. 특정 개체에게서 골 질량을 증가시키는 데에 유용한 화합물을 확인하기 위해 ｇｐｒ119 수용체를 사용하는 방법
AR064736A1 (es) 2007-01-04 2009-04-22 Prosidion Ltd Agonistas de gpcr
AR064735A1 (es) 2007-01-04 2009-04-22 Prosidion Ltd Agonistas de gpcr y composicion farmaceutica en base al compuesto
PT2114933E (pt) 2007-01-04 2011-12-20 Prosidion Ltd Agonistas do gpcr de piperidina
GB2456235B8 (en) * 2007-03-22 2009-12-09 Heptares Therapeutics Ltd Stable beta-adrenergic receptor mutants
JP2010539152A (ja) 2007-09-10 2010-12-16 プロシディオン・リミテッド 代謝障害の治療のための化合物
GB0720390D0 (en) 2007-10-18 2007-11-28 Prosidion Ltd G-Protein coupled receptor agonists
GB0720389D0 (en) 2007-10-18 2008-11-12 Prosidion Ltd G-Protein Coupled Receptor Agonists
EP2108960A1 (fr) 2008-04-07 2009-10-14 Arena Pharmaceuticals, Inc. Procédés d'utilisation d'un récepteur couplé à protéine G pour identifier les secrétagogues de peptide YY (PYY) et composés utiles dans le traitement des conditions modulées de secrétagogues BY (PYY) et composés utiles dans le traitement des conditions par PYY
GB0904284D0 (en) 2009-03-12 2009-04-22 Prosidion Ltd Compounds for the treatment of metabolic disorders
GB0904287D0 (en) 2009-03-12 2009-04-22 Prosidion Ltd Compounds for the treatment of metabolic disorders
GB0904285D0 (en) 2009-03-12 2009-04-22 Prosidion Ltd Compounds for the treatment of metabolic disorders
JP7422498B2 (ja) * 2019-07-25 2024-01-26 芳武 村山 ペプチド、細胞遊走抑制剤、細胞浸潤抑制剤、がん細胞転移抑制剤、及び医薬

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021925A1 (fr) * 1994-02-14 1995-08-17 American Cyanamid Company Recepteurs heterologues couples a des proteines g et exprimes dans la levure, leur fusion avec des proteines g et leur utilisation dans des dosages biologiques
WO1996000739A1 (fr) * 1994-06-29 1996-01-11 Merck & Co., Inc. Recepteurs modifies lies a des proteines g
WO1998038217A1 (fr) * 1997-02-27 1998-09-03 Milt Teitler Recepteurs de serotonine actives par constitution

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995021925A1 (fr) * 1994-02-14 1995-08-17 American Cyanamid Company Recepteurs heterologues couples a des proteines g et exprimes dans la levure, leur fusion avec des proteines g et leur utilisation dans des dosages biologiques
WO1996000739A1 (fr) * 1994-06-29 1996-01-11 Merck & Co., Inc. Recepteurs modifies lies a des proteines g
WO1998038217A1 (fr) * 1997-02-27 1998-09-03 Milt Teitler Recepteurs de serotonine actives par constitution

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
A. SCHEER ET AL: "Constitutively active G-protein-coupled receptors: Potential mechanisms of receptor activation", JOURNAL OF RECEPTOR & SIGNAL TRANSDUCTION RESEARCH, vol. 17, no. 1-3, 1997, pages 57 - 73, XP000867531 *
EGGERICKX D ET AL: "MOLECULAR CLONING OF AN ORPHAN G-PROTEIN-COUPLED RECEPTOR THAT CONSTITUTIVELY ACTIVATES ADENYLATE CYCLASE", BIOCHEMICAL JOURNAL,GB,PORTLAND PRESS, LONDON, vol. 309, 1995, pages 837 - 843, XP002912497, ISSN: 0264-6021 *
M.A. KJELSBERG ET AL: "Constitutive activation of the alpha1B-adrenergic receptor by all amino acid substitutions at a single site", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 267, no. 3, 25 January 1992 (1992-01-25), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD., US, pages 1430 - 1433, XP002135768, ISSN: 0021-9258 *
P. SAMAMA ET AL: "A mutation-induced activated state of the beta2-adrenergic receptor", JOURNAL OF BIOLOGICAL CHEMISTRY., vol. 268, no. 7, 5 March 1993 (1993-03-05), AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD., US, pages 4625 - 4636, XP002135769, ISSN: 0021-9258 *
PAUSCH M H ET AL: "HETEROLOGOUS G PROTEIN-COUPLED RECEPTORS EXPRESSED IN SACCHAROMYCES CEREVISIAE: METHODS FOR GENETIC ANALYSIS AND LIGAND IDENTIFICATION", IDENTIFICATION AND EXPRESSION OF G-PROTEIN-COUPLED RECEPTORS. CHAPTER II, 26 May 1999 (1999-05-26), Editedby Kevin R. Lynch, Wley-Liss, Inc., pages 196 - 212, XP000869632 *
PAUSCH M H: "G-protein-coupled receptors in Saccharomyces cerevisiae: high-throughput screening assays for drug discovery", TRENDS IN BIOTECHNOLOGY,GB,ELSEVIER PUBLICATIONS, CAMBRIDGE, vol. 15, no. 12, 1 December 1997 (1997-12-01), pages 487 - 494, XP004097426, ISSN: 0167-7799 *
PAUWELS P J ET AL: "REVIEW:AMINO ACID DOMAINS INVOLVED IN CONSTITUTIVE ACTIVATION OF G-PROTEIN-COUPLED RECEPTORS", MOLECULAR NEUROBIOLOGY,US,HUMANA PRESS, vol. 17, no. 1/03, 29 December 1998 (1998-12-29), pages 109 - 135, XP000866477, ISSN: 0893-7648 *

Also Published As

Publication number Publication date
AU756244B2 (en) 2003-01-09
CA2344591A1 (fr) 2000-03-09
EP1123391A2 (fr) 2001-08-16
WO2000012705A2 (fr) 2000-03-09
CA2343046A1 (fr) 2000-03-09
EP1112359A2 (fr) 2001-07-04
JP2002523091A (ja) 2002-07-30
JP2002523090A (ja) 2002-07-30
WO2000012705A3 (fr) 2000-10-05
AU5700999A (en) 2000-03-21
WO2000012704A9 (fr) 2000-06-08
WO2000012704A2 (fr) 2000-03-09
WO2000012705A9 (fr) 2000-07-13
AU5701199A (en) 2000-03-21

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