WO1998011259A3 - Composition, procede et necessaire pour la detection et l'identification de micro-organismes porteurs de maladies sexuellement transmissibles - Google Patents

Composition, procede et necessaire pour la detection et l'identification de micro-organismes porteurs de maladies sexuellement transmissibles Download PDF

Info

Publication number
WO1998011259A3
WO1998011259A3 PCT/CA1997/000660 CA9700660W WO9811259A3 WO 1998011259 A3 WO1998011259 A3 WO 1998011259A3 CA 9700660 W CA9700660 W CA 9700660W WO 9811259 A3 WO9811259 A3 WO 9811259A3
Authority
WO
WIPO (PCT)
Prior art keywords
sexually transmitted
transmitted disease
gene fragments
amplification
detection
Prior art date
Application number
PCT/CA1997/000660
Other languages
English (en)
Other versions
WO1998011259A2 (fr
Inventor
Max Chernesky
Kathleen Luinstra
Dan Jang
Sylvia Chong
James B Mahony
James M Dunn
Original Assignee
Visible Genetics Inc
Max Chernesky
Kathleen Luinstra
Dan Jang
Sylvia Chong
James B Mahony
James M Dunn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Visible Genetics Inc, Max Chernesky, Kathleen Luinstra, Dan Jang, Sylvia Chong, James B Mahony, James M Dunn filed Critical Visible Genetics Inc
Priority to AU41962/97A priority Critical patent/AU4196297A/en
Publication of WO1998011259A2 publication Critical patent/WO1998011259A2/fr
Publication of WO1998011259A3 publication Critical patent/WO1998011259A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

On procède à l'amplification conjuguée et à la détection d'au moins trois agents pathogènes de maladies sexuellement transmissibles dans l'ADN isolé dans un échantillon prélevé sur un patient, en combinant l'ADN isolé avec au moins une première, deuxième et troisième paire d'amorces oligonucléotidiques pour l'amplification de fragments de gènes à partir d'un premier, deuxième et troisième agent pathogène différent de maladies sexuellement transmissibles. L'une des amorces de chaque paire d'amorces est marquée au moyen d'un marqueur détectable. On met les première, deuxième et troisième paire d'amorces oligonucléotidiques en réaction avec l'ADN isolé dans une réaction d'amplification pour générer un mélange de produit d'amplification contenant des fragments de gène amplifiés de l'un du premier ou deuxième (ou des deux) agent(s) pathogène(s) de maladie sexuellement transmissible, selon que le premier et le second agent pathogène de maladie sexuellement transmissible sont présents dans l'échantillon ou non. On évalue ensuite les fragments de gène amplifiés dans le mélange de produit d'amplification, par exemple, par électrophorèse sur gel, pour déterminer si des fragments de gène amplifiés du premier, deuxième ou troisième agent pathogène de maladies sexuellement transmissibles sont présents.
PCT/CA1997/000660 1996-09-09 1997-09-09 Composition, procede et necessaire pour la detection et l'identification de micro-organismes porteurs de maladies sexuellement transmissibles WO1998011259A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU41962/97A AU4196297A (en) 1996-09-09 1997-09-09 Composition, method and kit for detection and identification of sexually transmitted disease microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70883296A 1996-09-09 1996-09-09
US08/708,832 1996-09-09

Publications (2)

Publication Number Publication Date
WO1998011259A2 WO1998011259A2 (fr) 1998-03-19
WO1998011259A3 true WO1998011259A3 (fr) 1998-04-23

Family

ID=24847349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA1997/000660 WO1998011259A2 (fr) 1996-09-09 1997-09-09 Composition, procede et necessaire pour la detection et l'identification de micro-organismes porteurs de maladies sexuellement transmissibles

Country Status (2)

Country Link
AU (1) AU4196297A (fr)
WO (1) WO1998011259A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8778611B2 (en) 2006-05-26 2014-07-15 The Secretary Of State For Health Mycoplasma genitalium detection assay based on the Mg219 gene

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE405674T1 (de) * 1998-09-24 2008-09-15 Innogenetics Nv Identizierung von microorganismen,die akute atemwegsinfektionen verursachen
KR100619189B1 (ko) * 2004-10-08 2006-08-31 굿젠 주식회사 성교전파성질환 원인균 탐지용 프로브 및 이를 이용한성교전파성질환 원인균 유전자형 분석용 ｄｎａ 칩,분석키트 및 유전형 분석방법
US20090035775A1 (en) * 2005-08-17 2009-02-05 University Of Delhi Pcr-based detection method for chlamydia treachomatis
US9139883B2 (en) 2005-08-17 2015-09-22 University Of Delhi PCR-based detection method for Chlamydia trachomatis
GB2431469A (en) * 2005-10-19 2007-04-25 Ivan Petyaev Pathogen diagnosis
KR100860619B1 (ko) * 2006-02-23 2008-09-29 주식회사 씨젠 성인성 질환 유발 병원체 핵산 검출용올리고뉴클레오타이드
ES2366798T3 (es) * 2006-05-29 2011-10-25 Bio-Rad Pasteur Detección múltiple en tiempo real de tres especies bacterianas responsables de enfermedades de transmisión sexual.
KR101101880B1 (ko) * 2008-11-25 2012-01-05 문우철 성교전파성질환 원인균의 탐지, 유전자형 분석 및 항생제 내성 유전자형의 분석용의 ｄｎａ 칩, 키트, 및 이것을 이용한 탐지 및 유전자형의 분석방법
GB201004235D0 (en) 2010-03-15 2010-04-28 Knight Scient Ltd Method for detection of inflammation in the urinary tract or urethra
KR20110105663A (ko) * 2010-03-19 2011-09-27 주식회사 진진바이오 멀티플렉스 리퀴드 어레이 시스템을 이용한 여성 생식기 병원체 검출 방법 및 키트
CN112458195B (zh) * 2020-12-28 2023-03-24 广州迈景基因医学科技有限公司 基于高通量测序检测性传播病原体的多重pcr引物组、试剂盒及其方法
CN113186316A (zh) * 2021-05-26 2021-07-30 北京华诺奥美基因生物科技有限公司 一种生殖支原体核酸实时荧光pcr检测引物、探针及试剂盒
CN113215322B (zh) * 2021-05-26 2022-09-30 北京毅新博创生物科技有限公司 Maldi-tof ms检测生殖道病原体的引物组合物及用途

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0648845A2 (fr) * 1993-07-08 1995-04-19 Johnson & Johnson Clinical Diagnostics, Inc. Procédé pour la coamplification de deux acides nucléiques différentes utilisant la réaction en chaîne de polymérase
EP0687737A1 (fr) * 1994-05-16 1995-12-20 F. Hoffmann-La Roche AG Détection du virus herpes simplex, treponema pallidum et haemophilus ducreyi

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0648845A2 (fr) * 1993-07-08 1995-04-19 Johnson & Johnson Clinical Diagnostics, Inc. Procédé pour la coamplification de deux acides nucléiques différentes utilisant la réaction en chaîne de polymérase
EP0687737A1 (fr) * 1994-05-16 1995-12-20 F. Hoffmann-La Roche AG Détection du virus herpes simplex, treponema pallidum et haemophilus ducreyi

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 125, no. 19, 4 November 1996, Columbus, Ohio, US; abstract no. 239517k, ZENG, XINGWU ET AL: "Rapid detection of mixed infection of Neisseria gonorrhoeae, Chlymidia trachomatis, Ureaplasma urealyticum by multiplex-PCR" page 271; column left; XP002056582 *
DE BARBEYRAC B ET AL: "Microbiological study of pelvic inflammatory disease (PID): Report on a french cohort", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 96, no. 0, 19 May 1996 (1996-05-19) - 23 May 1996 (1996-05-23), pages 286, XP002056566 *
MAHONY J ET AL: "Detection of C. trachomatis (CT), N. gonorrhoeae (NG), U. urealyticum (UU), M. genitalium (MG), in urine specimens by multiplex PCR (M-PCR)", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 96, no. 0, 19 May 1996 (1996-05-19) - 23 May 1996 (1996-05-23), pages 9, XP002056565 *
MITRANI-ROSENBAUM S ET AL: "SIMULTANEOUS DETECTION OF THREE COMMON SEXUALLY TRANSMITTED AGENTS BY POLYMERASE CHAIN REACTION", AMERICAN JOURNAL OF OBSTETRICS & GYNECOLOGY, vol. 171, no. 3, September 1994 (1994-09-01), pages 784 - 790, XP000612760 *
STEWART R ET AL: "Use of digoxigenin dUTP to increase detection sensitivity of PCR assays for common sexual diseases", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 94, no. 0, 23 May 1994 (1994-05-23) - 27 May 1995 (1995-05-27), pages 568, XP002056567 *
ZENG, XINGWU ET AL, ZHONGHUA YIXUE JIANYAN ZAZHI, vol. 19, no. 3, February 1996 (1996-02-01), pages 176 - 78 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8778611B2 (en) 2006-05-26 2014-07-15 The Secretary Of State For Health Mycoplasma genitalium detection assay based on the Mg219 gene

Also Published As

Publication number Publication date
AU4196297A (en) 1998-04-02
WO1998011259A2 (fr) 1998-03-19

Similar Documents

Publication Publication Date Title
Böhm et al. Real‐time quantitative PCR: DNA determination in isolated spores of the mycorrhizal fungus Glomus mosseae and monitoring of Phytophthora infestans and Phytophthora citricola in their respective host plants
Wiedmann-al-Ahmad et al. Characterization of Acinetobacter type strains and isolates obtained from wastewater treatment plants by PCR fingerprinting
WO1998011259A3 (fr) Composition, procede et necessaire pour la detection et l'identification de micro-organismes porteurs de maladies sexuellement transmissibles
AU9743398A (en) Detection and identification of human papillomavirus by pcr and type-specific r everse hybridization
FR2683827B1 (fr) Procede de determination de la quantite d'un fragment d'adn d'interet par une methode d'amplification enzymatique.
WO1995013396A3 (fr) Procede d'identification de micro-organismes, et dispositifs pour sa mise en oeuvre
EA200300991A1 (ru) Продукт, содержащий иммобилизованную нуклеиновую кислоту, полученный способом с участием химерного олигонуклеотидного праймера, днк-полимеразы и эндонуклеазы
EP0774518A3 (fr) Sondes d'acides nucléiques complémentaires aux acides nucléiques du virus du Papillome humain, procédés associés et trousse d'essais
WO1999028475A3 (fr) Sequence genomique et polypeptides de chlamydia trachomatis, leurs fragments et leurs utilisations, en particulier, pour le diagnostic, la prevention et le traitement de l'infection
WO1998048046A3 (fr) Amplification en chaine par polymerase-taqmantm pour detecter des souches pathogenes de e. coli
AU4391797A (en) The use of modular oligonucleotides as probes or primers in nucleic acid based assay
AU7104300A (en) Method for amplifying signal-flanking sequences from unknown genomic dna
AU682538B2 (en) Process for lysing mycobacteria
AU6975694A (en) Salmonella identification by the polymerase chain reaction
HK1056748A1 (en) Nucleic acid molecules for detecting bacteria and phylogenetic units of bacteria
ES2186745T3 (es) Procedimiento para la identificacion especifica en genero y especie de legionelas.
AU6715696A (en) Amplification and detection of mycobacterium avium complex species
AU2003289927A1 (en) Multiplex assay detection of pathogenic organisms
AU2002218129A1 (en) Method for the detection of cytosine methylations
WO1996015264A3 (fr) Procede d'identification de sequences nucleotidiques a partir de differentes sources biologiques par amplification des sequences
WO1995033854B1 (fr) Utilisation d'un marqueur specifique pour detecter la salmonella avec pcr
HK1001131A1 (en) Method for detecting a target nucleic acid
WO1996015264B1 (fr) Procede d'identification de sequences nucleotidiques a partir de differentes sources biologiques par amplification des sequences
CA2219458A1 (fr) Detection d'une sequence nucleotidique avec amplification de signal
EP0919633A3 (fr) Détection de Neisseria Gonorrhoeae par amplification et détection de ses acides nucléiques

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH KE LS MW SD SZ UG ZW AT BE CH DE DK ES FI FR GB GR IE IT LU MC

AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH KE LS MW SD SZ UG ZW AT BE CH DE DK ES FI FR GB GR IE IT LU MC

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: JP

Ref document number: 1998513087

Format of ref document f/p: F

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: CA