WO1997039326A3 - Dosage in vitro a polarisation de fluorescence - Google Patents
Dosage in vitro a polarisation de fluorescence Download PDFInfo
- Publication number
- WO1997039326A3 WO1997039326A3 PCT/US1997/006746 US9706746W WO9739326A3 WO 1997039326 A3 WO1997039326 A3 WO 1997039326A3 US 9706746 W US9706746 W US 9706746W WO 9739326 A3 WO9739326 A3 WO 9739326A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- proteins
- test substance
- emission
- mixture
- pair
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
Abstract
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP09530451A JP2000512737A (ja) | 1996-04-18 | 1997-04-18 | 試験管内蛍光偏光検定法 |
AU28076/97A AU727108B2 (en) | 1996-04-18 | 1997-04-18 | In vitro fluorescence polarization assay |
EP97922393A EP0898709A2 (fr) | 1996-04-18 | 1997-04-18 | DOSAGE $i(IN VITRO) A POLARISATION DE FLUORESCENCE |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US1559096P | 1996-04-18 | 1996-04-18 | |
US60/015,590 | 1996-04-18 | ||
US2987096P | 1996-11-06 | 1996-11-06 | |
US60/029,870 | 1996-11-06 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO1997039326A2 WO1997039326A2 (fr) | 1997-10-23 |
WO1997039326A3 true WO1997039326A3 (fr) | 1997-12-24 |
Family
ID=26687596
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1997/006746 WO1997039326A2 (fr) | 1996-04-18 | 1997-04-18 | Dosage in vitro a polarisation de fluorescence |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP0898709A2 (fr) |
JP (1) | JP2000512737A (fr) |
AU (1) | AU727108B2 (fr) |
CA (1) | CA2250067A1 (fr) |
WO (1) | WO1997039326A2 (fr) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0938586A4 (fr) * | 1996-10-28 | 2002-01-30 | Panvera Corp | Mesure de l'activite de la kinase par polarisation de fluorescence |
US7105307B2 (en) | 1997-08-30 | 2006-09-12 | Cyclacel, Ltd. | Compositions and methods for screening for modulators of enzymatic activity |
ATE317122T1 (de) | 1997-09-05 | 2006-02-15 | Matsushita Electric Ind Co Ltd | Fluoreszierende polarisation |
GB9923208D0 (en) * | 1999-10-01 | 1999-12-08 | Cambridge Drug Discovery Ltd | Assay |
UY27304A1 (es) | 2001-05-24 | 2002-12-31 | Avanir Pharmaceuticals | Inhibidores del factor inhibidor de la migración de los macrófagos y métodos para su identificación |
US7198958B2 (en) | 2001-07-02 | 2007-04-03 | Arctic Diagnostics Oy | Method for increasing hydrophilicity of fluorescent label compounds |
ATE447178T1 (de) * | 2001-07-02 | 2009-11-15 | Arctic Diagnostics Oy | Zwei photonen absorbierende bordipyrromethen- difluorid-farbstoffe und ihre anwendungen |
TW200418829A (en) | 2003-02-14 | 2004-10-01 | Avanir Pharmaceutics | Inhibitors of macrophage migration inhibitory factor and methods for identifying the same |
US7727752B2 (en) | 2003-07-29 | 2010-06-01 | Life Technologies Corporation | Kinase and phosphatase assays |
CA2531506A1 (fr) | 2003-08-22 | 2005-03-10 | Avanir Pharmaceuticals | Derives de naphtyridine substitues servant d'inhibiteurs de facteur d'inhibition de la migration des macrophages et leur utilisation pour traiter des maladies humaines |
JP4607950B2 (ja) * | 2004-03-18 | 2011-01-05 | トランス テック ファーマ,インコーポレイテッド | 蛍光偏光アッセイ |
US7608407B2 (en) * | 2004-11-16 | 2009-10-27 | Boehringer Ingelheim Pharmaceuticals, Inc. | Fluorescence polarization assay for determining histidine decarboxylase activity |
CA2600175A1 (fr) | 2005-03-24 | 2006-03-20 | Avanir Pharmaceuticals | Derives de thienopyridinone utilises en tant qu'inhibiteurs du facteur d'inhibition de la migration des macrophages |
WO2013056720A1 (fr) | 2011-10-18 | 2013-04-25 | Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. | Colorants fluorescents comportant des groupes hydroxyméthyle phosphorylés et leur utilisation en microscopie photonique et dans des techniques d'imagerie |
EP2944958A1 (fr) | 2014-04-04 | 2015-11-18 | Techno-Path (Distribution) | Procédé de prédiction d'instabilité phénotypique dans une cellule |
US20170160286A1 (en) * | 2014-07-15 | 2017-06-08 | Valitacell Limited | A Method of Measuring Antibody Concentration in a Sample |
EP3075844A1 (fr) | 2015-04-01 | 2016-10-05 | Valitacell Limited | Procédé de détermination d'une caractéristique fonctionnelle ou de composition d'un milieu de culture cellulaire |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4257774A (en) * | 1979-07-16 | 1981-03-24 | Meloy Laboratories, Inc. | Intercalation inhibition assay for compounds that interact with DNA or RNA |
EP0449269A1 (fr) * | 1990-03-27 | 1991-10-02 | Roche Diagnostics GmbH | Méthode pour la détection des protéines contenant tyrosine phosphorylée |
WO1993003377A1 (fr) * | 1991-07-31 | 1993-02-18 | Ziltener Hermann J | Procede de detection de restes de phosphotyrosine |
US5352660A (en) * | 1991-10-31 | 1994-10-04 | Mount Sinai Hospital Corporation | Method for assaying for a substance that affects a SH2-phosphorylated ligand regulatory system |
WO1997010253A1 (fr) * | 1995-09-15 | 1997-03-20 | Merck & Co., Inc. | Dosage a rendement eleve utilisant des proteines hybrides |
-
1997
- 1997-04-18 JP JP09530451A patent/JP2000512737A/ja active Pending
- 1997-04-18 EP EP97922393A patent/EP0898709A2/fr not_active Withdrawn
- 1997-04-18 WO PCT/US1997/006746 patent/WO1997039326A2/fr not_active Application Discontinuation
- 1997-04-18 AU AU28076/97A patent/AU727108B2/en not_active Ceased
- 1997-04-18 CA CA 2250067 patent/CA2250067A1/fr not_active Abandoned
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4257774A (en) * | 1979-07-16 | 1981-03-24 | Meloy Laboratories, Inc. | Intercalation inhibition assay for compounds that interact with DNA or RNA |
EP0449269A1 (fr) * | 1990-03-27 | 1991-10-02 | Roche Diagnostics GmbH | Méthode pour la détection des protéines contenant tyrosine phosphorylée |
WO1993003377A1 (fr) * | 1991-07-31 | 1993-02-18 | Ziltener Hermann J | Procede de detection de restes de phosphotyrosine |
US5352660A (en) * | 1991-10-31 | 1994-10-04 | Mount Sinai Hospital Corporation | Method for assaying for a substance that affects a SH2-phosphorylated ligand regulatory system |
WO1997010253A1 (fr) * | 1995-09-15 | 1997-03-20 | Merck & Co., Inc. | Dosage a rendement eleve utilisant des proteines hybrides |
Non-Patent Citations (2)
Title |
---|
B. A. LYNCH ET AL: "A fluorescence polarisation based Src-SH2 binding assay.", ANALYTICAL BIOCHEMISTRY., vol. 247, no. 1, 1997, NEW YORK US, pages 77 - 82, XP002045666 * |
P. WU ET AL: "A high-throughput STAT binding assay using fluorescence polatization.", ANALYTICAL BIOCHEMISTRY., vol. 249, no. 1, 1997, NEW YORK US, pages 29 - 36, XP002045667 * |
Also Published As
Publication number | Publication date |
---|---|
CA2250067A1 (fr) | 1997-10-23 |
EP0898709A2 (fr) | 1999-03-03 |
JP2000512737A (ja) | 2000-09-26 |
AU727108B2 (en) | 2000-11-30 |
WO1997039326A2 (fr) | 1997-10-23 |
AU2807697A (en) | 1997-11-07 |
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