CA2250067A1 - Dosage in vitro a polarisation de fluorescence - Google Patents

Dosage in vitro a polarisation de fluorescence Download PDF

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Publication number
CA2250067A1
CA2250067A1 CA 2250067 CA2250067A CA2250067A1 CA 2250067 A1 CA2250067 A1 CA 2250067A1 CA 2250067 CA2250067 CA 2250067 CA 2250067 A CA2250067 A CA 2250067A CA 2250067 A1 CA2250067 A1 CA 2250067A1
Authority
CA
Canada
Prior art keywords
protein
domains
proteins
test substance
domain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA 2250067
Other languages
English (en)
Inventor
Berkley A. Lynch
Ian A. Macneil
Mark J. Zoller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ariad Pharmaceuticals Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2250067A1 publication Critical patent/CA2250067A1/fr
Abandoned legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un procédé de dosage in vitro permettant d'identifier une substance test qui inhibe l'association mutuelle d'une paire de protéines. Ce procédé consiste à produire une paire de protéines aptes à s'associer, l'une des protéins étant porteuse d'une substande fluorescente liée de manière covalente; à préparer un mélange contenant les deux protéins et au moins une substance test; à irradier le mélange avec de la lumière polarisée d'une longueur d'onde appropriée permettant l'excitation de la substance fluorescente telle que l'indique l'émission de lumière polarisée; à mesurer le degré de polarisation de l'émission et à déterminer l'effet dû à la présence ou à la concentration de la substance test en réduisant la polarisation de l'émission observée d'un mélange de deux protéines seules. L'activité inhibitrice de la substance test est en corrélation avec les valeurs réduites de dépolarisation.
CA 2250067 1996-04-18 1997-04-18 Dosage in vitro a polarisation de fluorescence Abandoned CA2250067A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US1559096P 1996-04-18 1996-04-18
US60/015,590 1996-04-18
US2987096P 1996-11-06 1996-11-06
US60/029,870 1996-11-06

Publications (1)

Publication Number Publication Date
CA2250067A1 true CA2250067A1 (fr) 1997-10-23

Family

ID=26687596

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2250067 Abandoned CA2250067A1 (fr) 1996-04-18 1997-04-18 Dosage in vitro a polarisation de fluorescence

Country Status (5)

Country Link
EP (1) EP0898709A2 (fr)
JP (1) JP2000512737A (fr)
AU (1) AU727108B2 (fr)
CA (1) CA2250067A1 (fr)
WO (1) WO1997039326A2 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998018956A1 (fr) * 1996-10-28 1998-05-07 Panvera Corporation Mesure de l'activite de la kinase par polarisation de fluorescence
US7105307B2 (en) 1997-08-30 2006-09-12 Cyclacel, Ltd. Compositions and methods for screening for modulators of enzymatic activity
CN1157602C (zh) 1997-09-05 2004-07-14 松下电器产业株式会社 荧光偏振法检测样品的方法及其中使用的试剂
GB9923208D0 (en) * 1999-10-01 1999-12-08 Cambridge Drug Discovery Ltd Assay
MY140679A (en) 2001-05-24 2010-01-15 Avanir Pharmaceuticals Inhibitors of macrohage migration inhibitory factor and methods for identifying the same
ATE447178T1 (de) * 2001-07-02 2009-11-15 Arctic Diagnostics Oy Zwei photonen absorbierende bordipyrromethen- difluorid-farbstoffe und ihre anwendungen
DE60214709T2 (de) * 2001-07-02 2007-09-13 Arctic Diagnostics Oy Verfahren zur Erhöhung der Hydrophilie von Fluoreszenzmarker-Verbindungen
TW200418829A (en) 2003-02-14 2004-10-01 Avanir Pharmaceutics Inhibitors of macrophage migration inhibitory factor and methods for identifying the same
US7727752B2 (en) 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
WO2005021546A1 (fr) 2003-08-22 2005-03-10 Avanir Pharmaceuticals Derives de naphtyridine substitues servant d'inhibiteurs de facteur d'inhibition de la migration des macrophages et leur utilisation pour traiter des maladies humaines
EP1730526B1 (fr) * 2004-03-18 2010-01-06 Transtech Pharma, Inc. Analyse par polarisation de fluorescence
US7608407B2 (en) * 2004-11-16 2009-10-27 Boehringer Ingelheim Pharmaceuticals, Inc. Fluorescence polarization assay for determining histidine decarboxylase activity
CA2600175A1 (fr) 2005-03-24 2006-03-20 Avanir Pharmaceuticals Derives de thienopyridinone utilises en tant qu'inhibiteurs du facteur d'inhibition de la migration des macrophages
EP2768906B1 (fr) 2011-10-18 2019-02-27 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Colorants fluorescents comportant des groupes hydroxyméthyle phosphorylés et leur utilisation en microscopie photonique et dans des techniques d'imagerie
EP2944958A1 (fr) 2014-04-04 2015-11-18 Techno-Path (Distribution) Procédé de prédiction d'instabilité phénotypique dans une cellule
JP6629830B2 (ja) * 2014-07-15 2020-01-15 バリタセル リミテッド 試料中の抗体濃度を測定する方法
EP3075844A1 (fr) 2015-04-01 2016-10-05 Valitacell Limited Procédé de détermination d'une caractéristique fonctionnelle ou de composition d'un milieu de culture cellulaire

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
DE4009848A1 (de) * 1990-03-27 1991-10-02 Boehringer Mannheim Gmbh Verfahren zum nachweis von phosphoryliertes tyrosin enthaltenden proteinen
WO1993003377A1 (fr) * 1991-07-31 1993-02-18 Ziltener Hermann J Procede de detection de restes de phosphotyrosine
CA2054602C (fr) * 1991-10-31 2003-04-22 Anthony Pawson Methode de dosage d'une substance influant sur un systeme de regulation d'un ligand phosphoryle-sh2
JPH11513246A (ja) * 1995-09-15 1999-11-16 メルク エンド カンパニー インコーポレーテッド 融合タンパク質を用いるハイスループットアッセイ

Also Published As

Publication number Publication date
AU2807697A (en) 1997-11-07
JP2000512737A (ja) 2000-09-26
WO1997039326A3 (fr) 1997-12-24
WO1997039326A2 (fr) 1997-10-23
EP0898709A2 (fr) 1999-03-03
AU727108B2 (en) 2000-11-30

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