WO1997033895A1 - Molecules d'arn isolees se fixant a l'arginine et leurs applications - Google Patents
Molecules d'arn isolees se fixant a l'arginine et leurs applications Download PDFInfo
- Publication number
- WO1997033895A1 WO1997033895A1 PCT/EP1997/001223 EP9701223W WO9733895A1 WO 1997033895 A1 WO1997033895 A1 WO 1997033895A1 EP 9701223 W EP9701223 W EP 9701223W WO 9733895 A1 WO9733895 A1 WO 9733895A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- rna
- amino acid
- affinity
- rna molecule
- bound
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/30—Preparation of optical isomers
- C07C227/34—Preparation of optical isomers by separation of optical isomers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C273/00—Preparation of urea or its derivatives, i.e. compounds containing any of the groups, the nitrogen atoms not being part of nitro or nitroso groups
- C07C273/18—Preparation of urea or its derivatives, i.e. compounds containing any of the groups, the nitrogen atoms not being part of nitro or nitroso groups of substituted ureas
- C07C273/189—Purification, separation, stabilisation, use of additives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C277/00—Preparation of guanidine or its derivatives, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups
- C07C277/08—Preparation of guanidine or its derivatives, i.e. compounds containing the group, the singly-bound nitrogen atoms not being part of nitro or nitroso groups of substituted guanidines
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
Definitions
- This invention relates to isolated RNA molecules with high or
- the invention are processes lor securing these RNA molecules.
- a third example is the interaction of the H ⁇ V-1 TAT protein with a stem loop structure of TAR RNA, located
- TAR is a single arginine within a basic region of TAT.
- RNA to largely determine specificity, functionality, and
- RNA aptamers which specifically recognize amino acids
- arginine specific aptamers might be especially relevant to protein-RNA
- the HIV-1 Rev protein contains a basic region in which ten of seventeen amino acids between positions
- RRE responsive element
- TAR/ Tat complex an -irginine and an isoleucine residue were found to
- Rex-protein of HTLV-I might interact with its natural
- the present invention is a
- Figure 1 is a representative elution protocol obtained in
- the first pool (“pool 1" hereafter) was
- pool 2 was then subjected to PCR amplification.
- the PCR amplification was carrier out as described by Famulok, supra,
- amplification product was extracted with phenol/ CHCL y precipitated with ethanol, dissolved in 1.3 ml of TE (lOmM Tris-HCl, pH 7.6; ImM
- RNA produced in Example 1, supra was selected for high
- the preselection column consisted of
- RNA containing samples were added to the preselection column, and then washed with 2-3 volumes of buffer. The effect of this
- the column was then eluted with another 5 column volumes of binding buffer containing 20mM citrulline so as to remove any non-
- binding buffer plus 20mM arginine was then heat denatured, in
- RNA typically
- RNA molecules were used, i.e., Ag. 06 (SEQ ID NO: ), as well
- the value for Ag. 06 was about 330nM, i.e., nearly 200 times greater
- aminoglycoside antibiotics tobramycin, kanamycin, lividomycin
- RNA ribonucleic acid
- isolated ribonucleic acid molecules having
- affinities of around 300nM, more preferably greater than about 60 ⁇ M are affinities of around 300nM, more preferably greater than about 60 ⁇ M.
- RNA molecules can have the strong affinity for any L- or D-
- RNA molecules are those set forth in SEQ ID NOS: 3 through 20 , inclusive.
- RNA molecules have various uses, which will be clear to
- RNA molecules may be used to
- affinitv to form complexes of RNA and amino acid which can be
- Enantiomers of amino acids may be identified and/or
- amino acids such as L-arginine and L- ⁇ trulline may be separated, and
- the bound RNA is contacted with a solution of an amino acid which is not the amino acid for which a high affinity binder is
- steps using L-citrulline Preferably, one carries out the cycling for
- affinity binder is sought. This serves to elute the desired RNA from
- RNA be bound to the RNA
- a strong or high affinity binder as was noted, supra, is an RNA molecule which has a low Kd value.
- strong affinitv (or high affinity) binders are those with a Kd
- these molecules have a Kd
- this value is about 500 nM or less, and most
Abstract
L'invention, qui a trait à des molécules d'ARN isolées, dotées d'une affinité élevée pour des acides aminés particuliers, porte également sur des procédés permettant d'isoler ces molécules ainsi que sur leur utilisation. On isole ces molécules d'ARN par le biais d'une technique faisant intervenir différentes étapes de lavage et de réchauffage.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP9532275A JP2000507097A (ja) | 1996-03-12 | 1997-03-11 | アルギニンに結合する単離されたrna分子およびその使用 |
EP97915359A EP0888367A1 (fr) | 1996-03-12 | 1997-03-11 | Molecules d'arn isolees se fixant a l'arginine et leurs applications |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US1411396P | 1996-03-12 | 1996-03-12 | |
US014,113 | 1996-03-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1997033895A1 true WO1997033895A1 (fr) | 1997-09-18 |
Family
ID=21763618
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP1997/001223 WO1997033895A1 (fr) | 1996-03-12 | 1997-03-11 | Molecules d'arn isolees se fixant a l'arginine et leurs applications |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP0888367A1 (fr) |
JP (1) | JP2000507097A (fr) |
WO (1) | WO1997033895A1 (fr) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1992014843A1 (fr) * | 1991-02-21 | 1992-09-03 | Gilead Sciences, Inc. | Aptamere specifique de biomolecules et procede de production |
-
1997
- 1997-03-11 JP JP9532275A patent/JP2000507097A/ja active Pending
- 1997-03-11 WO PCT/EP1997/001223 patent/WO1997033895A1/fr not_active Application Discontinuation
- 1997-03-11 EP EP97915359A patent/EP0888367A1/fr not_active Withdrawn
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1992014843A1 (fr) * | 1991-02-21 | 1992-09-03 | Gilead Sciences, Inc. | Aptamere specifique de biomolecules et procede de production |
Non-Patent Citations (3)
Title |
---|
GEIGER A. ET AL.: "RNA Aptamers that bind L-arginine with sub-micromolar dissociation constants and high enantioselectivity" * |
GEIGER, ALBERT ET AL: "RNA aptamers that bind L-arginine with sub-micromolar dissociation constants and high enantioselectivity", NUCLEIC ACIDS RESEARCH, vol. 24, no. 6, 15 March 1996 (1996-03-15), pages 1029 - 1036, XP002037703 * |
GEIGER, ALBERT ET AL: "RNA aptamers that bind L-arginine with sub-micromolar dissociation constants and high enantioselectivity", NUCLEIC ACIDS RESEARCH, vol. 24, no. 6, 6 March 1996 (1996-03-06), XP002037702 * |
Also Published As
Publication number | Publication date |
---|---|
JP2000507097A (ja) | 2000-06-13 |
EP0888367A1 (fr) | 1999-01-07 |
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