WO1990005178A1 - Hybridome murin et anticorps diagnostique produit a partir d'un tel hybridome - Google Patents

Hybridome murin et anticorps diagnostique produit a partir d'un tel hybridome Download PDF

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Publication number
WO1990005178A1
WO1990005178A1 PCT/US1988/003858 US8803858W WO9005178A1 WO 1990005178 A1 WO1990005178 A1 WO 1990005178A1 US 8803858 W US8803858 W US 8803858W WO 9005178 A1 WO9005178 A1 WO 9005178A1
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cells
cell
monoclonal antibody
antibody
hybridoma
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PCT/US1988/003858
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English (en)
Inventor
James A. Radosevich
Steven T. Rosen
Yixing Ma
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Northwestern University
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Priority to PCT/US1988/003858 priority Critical patent/WO1990005178A1/fr
Priority to CA000583262A priority patent/CA1295271C/fr
Publication of WO1990005178A1 publication Critical patent/WO1990005178A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3023—Lung
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells

Definitions

  • the field of this invention is hybridomas and monoclonal antibodies. More specifically, this invention relates to hybridoma produced monoclonal antibody which identifies adenocarcino a surface antigens, and which is useful in the diagnosis of bronchopulmonary and non-pulmonary carcinomas.
  • hybrid cell lines of the type described above comprises-the steps of immunizing a animal (usually a rat or mouse, but not necessarily one of these) with an antigen to which a monoclonal antibody is required.
  • the animal After allowing time for the immune system to generate lymphocytes secreting the antibodies to the antigen, the animal is sacrificed and a suspension of spleen cells is prepared. Fusion between these cells and myeloma cells is achieved by bringing them into contact in the presence of a fusion promoter (e.g. polyethylene glycol) . A small percentage of the cells fuse to produce hybrid myeloma cells.
  • a fusion promoter e.g. polyethylene glycol
  • a small percentage of the cells fuse to produce hybrid myeloma cells.
  • the immunization results in a plurality of different lymphocytes each secreting antibody to a different antigenic determinant, and these characteristics are transferred genetically to the hybrid cells. It is possible, by careful screening, to isolate from a culture of hybrid cells, a cell having the desired specificity. Such cells may be closed and cultured.
  • the advantage of this technique is that it provides a source of a specific antibody uncontaminated by antibodies raised to other determinants either on the antigen with which the mammal was immunized or on antigenic impurities in the immunizing material.
  • Another advantage of the technique is that antigen not available in a pure form for screening assays and present in the immunizing material at low concentrations, maybe used.
  • the present invention concerns a monoclonal antibody to bronchopulmonary carcinomas that may classify various bronchopulmonary carcinomas and identify one specific lung carcinoma type, adenocarcinoma, while additionally identifying in non-pulmonary sites.
  • bronchopulmonary neoplasms encompass several types: squamous cell carcinoma (SCC), " adenocarcinoma (AC), adenosquamous carcinoma (ASC), large cell carcinoma (LCC), bronchioloalveolar carcinoma (BAC), small cell neuroendocrine carcinoma (SCNC), intermediate cell neuroendocrine carcinoma (ICNC), well-differentiated neuroendocrine carcinoma (WDNC), carcinoid, and other rare entities such as mucoepidermoid carcinoma, adenoid cystic " carcinoma and carcinosarcoma.
  • SCC squamous cell carcinoma
  • AC adenocarcinoma
  • ASC large cell carcinoma
  • BAC bronchioloalveolar carcinoma
  • SCNC small cell neuroendocrine carcinoma
  • ICNC intermediate cell neuroendocrine carcinoma
  • WDNC well-differentiated neuroendocrine carcinoma
  • carcinoid and other rare entities such as mucoepidermoid carcinoma, adenoid cystic " carcinoma and carcinosarcom
  • bronchopulmonary refers to the bronchial tubes, pulmonary system and lungs.
  • carcinomas are often difficult to interpret because of the rapidly changing art being developed to classify carcinomas. As more characteristics of carcinomas are discovered, they may be reclassified into different groups.
  • Hybridoma HB 8986 The hybridoma clone, HB 8986, was produced from the fusion of primed mouse splenocytes and mouse myeloma cells.
  • Hybridoma HB 8986 produces a murine IgG ⁇ monoclonal antibody which recognizes an antigen on the cell surface of adenocarcinoma of the lung and on non-pulmonary sites.
  • Immunoperoxidase staining with HB 8986 on formalin fixed, paraffin embedded tissues shows that it specifically stains both the cytoplasmic and cell surface.
  • the antibody produced from HB 8986 generally reacts with and causes the staining of subset of neoplasms presently known as adenocarcinomas.
  • Certain human adenocarcinomas including pulmonary adenocarcinoma react with the monoclonal antibody.
  • the monoclonal antibody has the ability to distinguish lung carcinomas with glandular differentiation from other lung carcinomas lacking these features, and it may be useful as both a diagnostic and therapeutic agent.
  • Adenocarcinomas of the stomach, colon, pancreas, gall bladder and breast also react with the monoclonal antibody.
  • the antigenic preparation used in obtaining the hybridoma HB 8986 consisted of the well characterized human adenocarcinoma cell line, A549 available from the American National Cancer Institute Culture Collection, Rockville, Maryland. Briefly, the hybridoma clone HB 8986 was produced by the fusion of mouse myeloma cell line SP2/0.Agl4 and Balb/c splenocytes obtained from a mouse hyperimmunized with the human adenocarcinoma cell line, A549. Polyethylene glycol was used as the fusion agent. Only one hybrid line was derived from any one of the original 480 wells used in plating the hybrids after the fusion. The hybridoma producing monoclonal antibody was selected after initial screening and was subsequently cloned three times in soft agar.
  • Ascites fluid prepared by intraperitoneal implantation of 1 x 10 7 hybridoma cells in the Balb/c mice which have been pristane-primed three to seven days prior to injection.
  • the monoclonal antibody purified by 50% ammonium sulfate precipitation followed by affinity chromatography using Staphyloccocus Protein-A. Protein concentrations were determined using the Bio-Rad protein assay kit using bovine plasma gamma globulin as a standard. Supernatants, ascites fluids, and purified antibodies samples were stored with a final concentration of 0.02% sodium azide or sterile filtered, at either 4°C or minus 70°C.
  • hybridoma HB 8986 The monoclonal antibody produced by the hybridoma HB 8986 was tested to determine its properties and specificity of the HB 8986 antibody molecules. These tests and results are described below.
  • the primary morphological-characteristics of hybridoma HB 8986 are as follows:
  • Origin It was produced by fusion of SP2/0.Agl mouse myeloma cells with Balb/c mouse splenocytes primed with human adenocarcinoma cell line A549.
  • the hybridoma grows in 10% Fetal Calf Serum/90% RPMI-1640 at 37°C, with 5% C0 2 /95% air.
  • the hybrid can be adapted to a variety of other media and growth conditions.
  • Frozen storage of the cell line may be in either 10% Dimethylsulfoxide or 10% glycerine.
  • the HB 8986 hybridoma is not phytopathogenic and has not known to have any dangerous properties. It is tumorigenic in Blab/c mice.
  • the HB 8986 hybridoma produces a murine IgG- j _ monoclonal antibody which stains the cytoplasm and cell surface of some fixed tissue (i.e. formalin) paraffin embedded human lung adenocarcinomas.
  • HB 8986 antibody by the hybridoma cells can be tested ' by the Avidin Biotin Complex Method or by a variety of other methods on formalin fixed paraffin embedded human bronchopulmonary carcinomas, or on cell lines. Alternatively RIA methods may be used.
  • the HB 8986 hybridoma may be propagated in vitro at an initial cell concentration of one cell per flask.
  • the cells are grown in stationary suspension culture up to 3 x 10 6 cells/ml.
  • the HB 8986 antibody may also be produced.
  • the antibody is obtained by centrifuging to remove the cells, and saving the supernatant.
  • the hybridoma may be injected into the peritoneal cavity of pristane primed Balb/c mice.
  • the injected hybridoma will cause formation of ascites in the peritoneal cavity, which will result in fluid containing a high concentration of the desired antibody.
  • Antigen preparation The human lung carcinoma cell line A549 was harvested at mid-log growth phase using a rubber policeman to dislodge the cells from the flask. The cell suspension was triturated gently and washed three times with PBS. The cells were then resuspended in 0.5 ml of PBS and an emulsion of Freund's complete or incomplete adjuvant was prepared by sonication.
  • Immunization protocol The mouse was injected on day 1 with 1 ml of emulsion using Freund's complete adjuvant emulsion. Days 7, 14 and 21 with 1 ml of Freund's complete adjuvant emulsion. The mouse was then rested for four weeks and boosted 3 days prior to fusion with Freund's incomplete adjuvant emulsion.
  • Serologic characterization Prior to splenectomy, a small sample of serum was obtained from the mouse, and tested by radioimmunoassay (as described below) for the presences of antibodies reacting with the human lung carcinoma cell line, A549.
  • Splenocyte preparation On the day of the fusion, a splenectomy was performed on the mouse. The spleen was then teased under sterile conditions in RPMI-1640.
  • Cell fusion Washed splenocytes and SP 2/0.Agl4 were combined at a ratio of 2:1. One ml of 50% PEG was added dropwise to the cells over a 60 second period. After an additional 60 seconds, 5 mis of RPMI-1640 was used to dilute the cells slowly. An additional 10 mis of RPMI-1640 was then added slowly over a ten minute period.
  • Cloning Cells which yielded positive antigen binding supernatants were selected to be clone on soft agar. The cells from each individual cell expanded and 100 ul of a 13 mm soft agar plate. After 3-7 days, individual colonies were picked, grown, and tested by RIA. Each time the most positive clone was chose. The process was repeated a total of three times.
  • Human lung carcinoma cell line lysates were made by traditional methods. Supernatants, ascites fluid, or purified antibody (50 ul of each) was incubated at room temperature for one hour in 96 well polyvinyl chloride target plates of each cell line tested. After being washed seven times with 250 ul volumes of phosphate buffered saline (PBS), the target plates were incubated with 5 x 10 ⁇ cpm of - • ⁇ "I-sheep-anti-mouse F(ab')2 fragments in a 50 ul volume. The plates were washed seven times as stated above after a one hour incubation, and counted in a gamma counter for one minute.
  • PBS phosphate buffered saline
  • Human lung carcinoma cell lines were harvested at mid-log growth phase using a rubber policeman to dislodge cell lines which adhere to the flasks. Cell suspensions were triturated gently and washed three times with PBS. 1 x 10- ⁇ cells were resuspended in 100 ul of PBS containing 0.02% sodium azide and plated into microtiter wells. The microtiter plates had been pre-treated for 24 hours with 1 mg/ml BSA in PBS with azide. Fifty ul of supernatant or ascites fluid (diluted 1:1000) was added to each well. Plates were incubated at room temperature for thirty minutes on a platform shaker set at low speed.
  • the cells were washed seven times with cold PBS containing azide by centrifuging the plates at 1,000 rpm for 5 minutes, aspirating the supernatants, and resuspending the cells in 200 ul of PBS.
  • -"-- ⁇ I-sheep-anti-mouse antibody F(ab')2 (50,000 cpm/50 ul) was added to each well after being washed and incubated as before. Each well was again washed seven times as before, and the wells counted in a gamma counter for one minute intervals.
  • Nude mouse xenografts of human lung adenocarcinoma cancer cell lines A549, SKLU-1 available from the American Type Culture Collection, Rockville, Maryland 20852 and human small cell lung carcinoma cell lines NCI-H69 and NCI-H60 available from the National Cancer Institute were fixed in 10% formalin and paraffin embedded using traditional methods. Material was excluded if autolysis was noted. Simultaneous analysis of an identical hematoxylin and eosin stained tissue section was performed. All tissue sections were 7um thick and were prepared as in a traditional method with the primary antibody being the monoclonal antibody culture supernatants.
  • Immunoperioxidase staining using the Avidin-Biotin-Horseradish peroxidase complex method was used as suggested by the manufacturer, and counter-stained with hematoxalin. Positive tissue was tested with each set of slides and controls included no primary antibody or an antibody of the same isotope but different binding characteristics.
  • the monoclonal antibody of this invention was produced by hyperimminizing a Balb/c mouse with a human adenocarcinoma (AC) cell line A549.
  • a stable hybridoma was obtained using SP-/0.Agl4 as a fusion partner.
  • the monoclonal antibody is an IgG-*_ isotype and binds Staphylococcus protein A.
  • a solid phase radioim unoassay (RIA) this antibody reacted with several human lung adinocarcinoma cell lines, but not with several human small cell lung cancer (SCLC) cell lines.
  • Live cell RIA, immunofluorescent studies and radioimmunodection of nude mouse AC xenografts indicate that the antigen recognized by the monoclonal antibody is on the cell surface.
  • the antigen is preserved after formalin fixation and paraffin embedding as shown by the immunoperoxidase staining.
  • Human AC biopsy specimens react with the monoclonal antibody, while human SCLC biopsy specimens do not react.
  • Live cell radioim unoassay data is shown in Table 1 as both total cpm and binding ratios. To confirm that the antibody was binding to a cell surface component, it was tested on both live and fixed human lung cancer cell lines using immunofluorescences. The adenocarcinoma line, A549 revealed cell surface binding where the remaining cell lines tested were all negative.
  • Xenograft tumor tissue of the human lung cancer cell line A549 was positive. Staining was both cytoplasmic and cell surface. While xenograft tumor tissue of human lung cancer cell line NCI-H60 did not stain, similar results were found with other small cell and adenocarcinoma cell line xenografts.
  • Cytologic preparation including bronchial brushings or fine needle aspirates, amy be the earliest, or only, diagnostic material available at the time therapeutic decisions are made.
  • the overall diagnostic accuracy for detecting malignancy in bronchial brushings 89.6% for central and 70.9% peripheral lesions as referenced in Cytology of the Lung, Techniques and Interpretation, New York, Igaku-Shoin, 1983, pp. 25, 29, 75-131, but the accuracy in assigning histologic types is lower, especially for a large cell undifferentiated carcinoma.
  • SCC Squamous Cell
  • AC Adenocarcinoma
  • LCUC Large Cell Undifferentiated
  • NEC Neurendocrine Carcinoma
  • Tissue specimens were formalin fixed paraffin embedded, and stained with HE for light microscopy. Immunohistochemistry was performed on deparaffinized sections cut from the same blocks. Cytologic specimens consisted of direct smears of brushings made at the time of bronchoscopy; these were fixed immediately in 95% alcohol and stained by the Papanicalou technique. Two slides with numerous well preserved tumor cells distributed both singly and in clusters were selected from each case. Immunohistochemistry was performed directly on one slide, the other was destained first by passing it through graded alcohols. Immunostaining was performed with the Avidin-biotin complex method (Victor Laboratories Burlingame, California). The monoclonal antibody was used at a concentration of approximately 1 ug/ml. Slides were slightly counterstained with hematoxylin for 1.5 minutes, hydrated and mounted conventionally. Negative controls were performed by omitting primary antibody and substituting a nonreactive monoclonal antibody of the same isotype.
  • Tissue specimens consisted of 6 biopsies, 5 resections, and one biopsy of a chest wall metastasis. One patient had received radiation therapy prior to both brushings and resection. Microscopically the tumors, as defined, exhibited gland formation in over 50% of the sample. Cells were of intermediate or large size, with vesicular nuclei and prominent eosinophilic nucleoli. The radiated case showed extensive necrosis and marked stromal fibrosis. Immunohistochemical staining was strongly and diffusely positive in ten cases. In two cases, tumor cells showed variably intense positivity, with some cells exhibiting only faint staining. One of these was the radiated case, and the other was a bronchioloalveolar carcinoma. In one additional case, a few foci of squamous differentiation were notably negative.
  • tumor cell clusters were easier to identify than single cells and more reliable to evaluate for positivity due to better preservation of the cytoplasm within the clusters.
  • all tumor cells were strongly positive.
  • the remaining two cases showed mostly positive and occasional negative clusters.
  • Tissue specimens included 5 biopsies and 2 lobectomies; one of the latter was performed after radiation therapy. Microscopically, the tumors consisted of nests and sheets of large cells with marked nuclear pleomorphism, prominent eosinophilic nucleoli, and abundant eosinophilic cytoplasm. Mitoses were frequent. The previously radiated tumor displayed extensive necrosis. Four cases exhibited focal formation of gland lumens while two were felt to have some squamous features.
  • Immunostaining was strong and diffuse in two cases, and focal in two cases; three cases did not immunostain.
  • the negative cases included one with features of adenocarcinoma.
  • Immunohistochemical staining was strongly and diffusely positive in 5 cases, variable in one case, and negative in 1 case.
  • the negative case also had negative histology, but showed questionable features of adenocarcinoma both cytologically and histologically.
  • Tissue specimens included 7 biopsies and one lobectomy. Six cases were diagnosed as neuroendocrine carcinoma, small cell type, and two as intermediate cell type. The latter pair included the case with lobectomy, which was performed for the initial diagnosis of adenocarcinoma. The diagnosis was subsequently revised after examination of more abundant tissue from the lobectomy; electron microscopic study of this case showed prominent membrane bound granules.
  • the small cell carcinomas were present as sheets of cells underneath the bronchial mucosa. Necrosis and crushing artifact were prominent. Preserved nuclei were round, oval or fusiform. Nuclear molding, sparse cytoplasm and inconspicuous nucleoli were distinctive features.
  • the intermediate cell NE carcinomas consisted of larger cells whose pleomorphic nuclei had more stippled chromatin and a more distinct cytoplasmic rim.
  • Immunohistochemical staining was negative in all but the resected case of intermediate cell neuroendocrine carcinoma, where a few cells were positive.
  • Tissue specimens consisted of 6 biopsies and two resections. The tumor was well differentiated in 1 case, moderately differentiated in 6 cases, and poorly differentiated in one case.
  • Accuracy in correlating cytologic and histologic typing of bronchopulmonary tumors ranges from 75 to 94% for SCC, 68-86% for AC, 42 to 91% for LCUC and 83 to 96% for small cell NEC, according to Cytology of the Lung Techniques and Interpretation, New York, Igaku-Shoin, 1983, pp. 25, 29, 75-131; Am. J. Phthol. 1976 84:372-424; and ACTA Cvto 1981 25:499-505.
  • cytology-histology correlation was 100% for AC, with the caveats that in four cases cytology samples were diagnosed as: "poorly differentiated carcinoma with features of adenocarcinoma” and one case was diagnosed as "large cell undifferentiated carcinoma with features of adenocarcinoma”. Correlation for LCUC was 75% accurate; two cases were called “adenocarcinoma” on brushings. Correlation for NEC was called “adenocarcinoma” on brushings while the other was called “small cell NEC”. Accuracy and correlation for SCC was 88%; one case had been diagnosed as "large cell undifferentiated carcinoma” on brushings.
  • the monoclonal antibody of the present invention recognizes an antigen as a broad distribution and expression pattern. Immunoreactivity was found in adenocarcinomas of the gastral intenstional tract, although only a subset of the tumors showed positive staining. It is believed that this subpopulation may represent more aggressive tumors since many of the tumors which showed reactivity did so in areas which were less differentiated and lacked a distinct glandular pattern. Outside the gastrointestinal tract, breast adenocarcinomas showed the most consistant staining pattern. However, overall the normal adult distribution of the antigen recognized by the monoclonal antibody is limited. The monoclonal antibody may be useful in immunotherapy, radioimmunotherapy and radioimmuno detection.
  • adenocarcinomas arising in the endometrium, ovary kidney, prostate, thyroid and liver were either negative or showed very vocal reactivity. Strong staining was noted in adenocarcinomas which had an undifferentiated infiltrating component. Immunoreactivity- was noted in epithelial cells from the normal bronchial tract, stomach, small intestine, pancreas and colon, wherein cells from the endometrium, kidney, ovary, prostate and thyroid were negative.
  • the monoclonal antibody of the present invention is a useful immunodiagnostic probe capable of distinguishing among a number of normal as well as transformed glandular epithelia.
  • neoplasms selected for the study (the number of cases used in parentheses) whereas follows: adenocarcinomas of the breast (22); stomach (20); colon-rectum (25); small bowel (2); gallbladder (4); endometrium (12); ovary (8); pancreas (2); and prostate (10); islet cell neoplasms (6); renal cell carcinoma (8) ; adrenal cortical carcinoma (2) ; pheochromocytoma (2) . Material was also submitted from the City of Hope National Medical Center and consisted of breast carcinomas (71) and thirty adenocarcinomas from sites other than the lung.
  • Adenocarcinomas of the stomach, pancreas and gall ⁇ bladder showed strong cytoplasmic and/or membrane staining in a subset of tumors as noted in Table 3.
  • breast carcinomas of all major types show the most frequent and in some cases the strongest reactivity of the neoplasm study.
  • Epithelial cells in the malignancy studied showed strong cytoplasmic and/or membrane staining. This pattern was consistently reported in cells of intraductal, infiltrating ductal, lobular, mudullary and colloid carcinomas. The well differentiated and poorly differentiated areas showed similar reactivity.
  • a hybridoma capable of producing an antibody against an antigen found on adenocarcinoma cells and the A549 cell line; a method of producing the hybridoma, and a method of producing the monoclonal antibody.
  • the present invention encompasses all monoclonal antibodies exhibiting the characteristics described herein, it was determined that the subject antibody belongs to the subclass IgG- ⁇ , which is one of four subclasses of murine IgG.
  • the subclasses of immune globulin G differ from one another in the so-called "fixed” regions, an antibody to a specific antigen will have a so-called "variable' region.
  • a monoclonal antibody exhibiting the characteristic described herein may be of a subclass IgG-j_, IgG 2a , IgG 2b' I( 3 G 3 re of classes IgM, IgA, IgE, or IgD.
  • the differences in one of these classes or subclasses will not affect the selectivity of the reaction pattern of the antibody but may affect the further reaction of the antibody with other materials, such as complement.
  • the subject antibody is specifically IgG- ⁇ , it is contemplated that the antibodies having the pattern of the ' reactivity illustrated herein are included within this subject invention regardless of the immune globulin class or subclass to which they belong.
  • the present invention additionally includes diagnostic and therapeutic compositions comprising a diagnostically effective or therapeutically effective amount of the monoclonal antibody in a diagnostically or pharmaceutically acceptable carrier.

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Abstract

L'hybridome HB 8986 produit un anticorps monoclonal murin qui reconnaît un nouveau déterminant exprimé dans les carcinomes broncho-pulmonaires et dans les tumeurs dérivées des lignées cellulaires A 549. L'immunoperoxydase déteignant avec l'hybridome sur des tissus imprégnés de paraffine et fixés par formaline montre que cette substance colore spécifiquement à la fois la surface du cytoplasme et à la fois la surface des cellules. L'anticorps monoclonal a le pouvoir de distinguer de préférence les carcinomes broncho-pulmonaires avec différenciation glandulaire des autres carcinomes broncho-pulmonaires. L'anticorps monoclonal décrit peut en outre identifier la présence d'adénocarcinomes dans le corps.
PCT/US1988/003858 1988-10-31 1988-10-31 Hybridome murin et anticorps diagnostique produit a partir d'un tel hybridome WO1990005178A1 (fr)

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PCT/US1988/003858 WO1990005178A1 (fr) 1988-10-31 1988-10-31 Hybridome murin et anticorps diagnostique produit a partir d'un tel hybridome
CA000583262A CA1295271C (fr) 1988-10-31 1988-11-16 Hybridome murin et utilisation de l'anticorps produit par cet hybridome a desfins diagnostiques

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0454338A1 (fr) * 1990-04-16 1991-10-30 Duke University Méthode de diagnostic de cancer

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987004183A1 (fr) * 1986-01-07 1987-07-16 Univ Northwestern Hybridome de souris et anticorps de diagnostic produit avec celui-ci

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1987004183A1 (fr) * 1986-01-07 1987-07-16 Univ Northwestern Hybridome de souris et anticorps de diagnostic produit avec celui-ci

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CANCER RESEARCH, Vol. 45, No. 11, part 2, issued November 1985 (Baltimore, Maryland, USA); J.A. RADOSEVICH et al.: "Monoclonal Antibody 44-3A6 as a Probe for a Novel Antigen Found on Human Lung Carcinomas with Gladular Differentiation", pages 5808-5812, see abstract. *
CANCER RESERACH, Vol. 45, No. 11, part 2, issued November 1985 (Baltimore, Maryland, USA); I. LEE et al.: "Immunochemical Analysis of Human Pulmonary Carcinomas Using Monoclonal Antibody 44-3A6", pages 5813-5817, see abstract. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0454338A1 (fr) * 1990-04-16 1991-10-30 Duke University Méthode de diagnostic de cancer

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