WO1988002600A1 - Composition bactericide contenant une enzyme et preparation pour le traitement d'infections notamment dentaires comprenant ladite composition - Google Patents
Composition bactericide contenant une enzyme et preparation pour le traitement d'infections notamment dentaires comprenant ladite composition Download PDFInfo
- Publication number
- WO1988002600A1 WO1988002600A1 PCT/DK1987/000130 DK8700130W WO8802600A1 WO 1988002600 A1 WO1988002600 A1 WO 1988002600A1 DK 8700130 W DK8700130 W DK 8700130W WO 8802600 A1 WO8802600 A1 WO 8802600A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- composition
- enzyme
- wound treatment
- peroxidase
- glucose
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/44—Oxidoreductases (1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/44—Oxidoreductases (1)
- A61K38/443—Oxidoreductases (1) acting on CH-OH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/47—Hydrolases (3) acting on glycosyl compounds (3.2), e.g. cellulases, lactases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
Definitions
- This invention relates to a bactericidal composition of the type containing at least one enzyme which in aqueous milieu and in the presence of oxygen and a substrate may form oxygen peroxide together with a peroxidase and a thiocyanate.
- the bactericidal composition according to the invention is generally applicable, but as the composition is primarily developed and tested with respect to preparing a composition for the prevention and treatment of gingivitis and paradentosis, the invention will be more particularly explainde in the following with a view to obtaining a solution of this problem.
- dental plaque depositing on the tooth surfaces as bacterial coatings which contain more or less protein and carbon hydrate substances, epithelial cells and food debris or stimulant debris are sharing the responsibility for caries, dental calculus, gingivitis and paradentosis.
- Certain of the bacteria synthesize some strongly adhesive carbon hydrates (dextrans) from carbon hydrates of the intaken foodstuffs or stimulants and particularly sucrose constitutes the major basis of the formation of the dextrans.
- the bacteria in plaques develop organic acids, in particular lactic acid and acetic acid due to the fermentative decomposition and reaction of the particularly decomposable mono- and disaccharides occurring in or developed from intaken foodstuffs and stimulants.
- Dental calculus occurs as a cause of a minirali zation of dental plaque having a tendency to accumulate calcium and phosphate. If dental plaques are not regularly removed, e.g. by tooth brushing the accumulation of calcium and phosphates will reach a stage where a coherent and wear-resisting network of hydroxy apatite embedding the bacteria develops on the surfaces of the teeth.
- the progressing dental calculus in which hydroxy apatite amounts to 80% of the total material of dental calculus has a tendency to grow, because the bacteria in dental calculus cannot be removed by ordinary tooth brushing.
- dental calculus develops typically in areas of the teeth that are difficult to clean by tooth brushing. In this context the tooth surfaces facing the spaces between the teeth are particularly important. Dental calculus deposited between the tooth spaces will be spread therefrom and further on to the spaces between the teeth and gingivae, following which the dental calculus under gingivae will be spread from the tooth spaces to other areas of the teeth. In more advanced stages the dental calculus occurs on large areas between gingivae and the neck of the teeth and the organic acids generated by the acids will not only demineralize the teeth but also the bone structures located behind gingivae.
- Said chemical compounds are said to derange, thereby confining the formation of dextran gels from sucrose caused by the bacteria.
- the method does not have the intended effect on bacterial coatings in the teeth spaces where plaques are difficult to remove and where adhering food residues most frequently ensure that the bacteria have free access to low-molecular carbon hydrates.
- the method does not have any effect on already developed dental calculus and, therefore, this method is held to be less suited with respect to the inhibition of gingivitis and paradentosis.
- dextran gels generated in plaques are according to the accepted German patent application No. 1,955,956 proposed to be broken down by dextranases. According to AT patent No. 318,815 it has, however, been found that dextranase was merely capable of breaking down soluble dextran, while in plaques there is some of the insoluble dextran that cannot be broken down. According to US patent No. 4,154,815 dental plaque is confined by making use of zinc in connection with proteases, carbohydrases and/or Upases. Zinc has been found to be active by interference with the plaque structure and the mineralization of plaques, while the enzymes are entitled to decompose the plaques densifying high-polymeric compositions. A clinical experiment referred to in said patent demonstrates that zinc and protease were capable of reducing the amount of plaques on teeth surfaces, but the experimental report says nothing about the effect in the spaces between the teeth and on dental calculus.
- DK patent application No. 5504/78 discloses a caries inhibiting dental hygiene agent in tablet form or powder form, said agent containing only lysozyme as an active component. This reference does not specify the type of lysozyme applied.
- dental hygiene agents regularly used for long periods of time include antibiotics interfering in the intended microflora balance within the mouth, e.g. in connection with developing resistance.
- German patent application No. 2,027,019 and the parallel US patent No. 4,150,113 deal with a dental hygiene agent containing an addition of oxidoreductase which by oxidative decomposition of a substrate causes hydrogen peroxide to be liberated. This is asserted to provide for obtaining a caries inhibiting effect.
- the glucose acting as a substrate is said to be obtained from food debris in the mouth containing for example starcj or desaccharides, becouse the centifrice may as well contain carbohydrases generating glucose from the poly- or disaccharides concdrned.
- EP patent application No. 133,736 relates to dentifrice compositions having an antibacterial activity and are based on the formation of hypothiocyanate (OSCN-) during utilization.
- the agents concerned contain an oxidable substrate and an oxidoreductase system specific of said substrate and which in the presence of oxygen in aqueous milieu generates hydrogen peroxide which by reaction wit ⁇ the thiocyanate that is further contained in the agent generates hypothiocyanate under the influence of lactose peroxidase.
- the agents contain for example glucose as an oxidable substrate, which is oxidized with glucose oxidase under the formation of hydrogen peroxide reacting with thiocyanate (SCN-) added for instance in the form of potassium thiocyanate, and with lactoperoxidase to produce the bacteriostatic hypothiocyanate (OSCN-).
- SCN- thiocyanate
- OSCN- lactoperoxidase
- the agent is proposed to be used in inhibiting caries and for that purpose a bacteriostatic activity may per se provide for obtaining an advantageous effect.
- a bactericidal activity is, however, not sufficient, since bacterial plaques in the spaces between the teeth and in gingival pockets will continue their growth upon decline of the inhibiting efficacy of the hypothiocyanate.
- hypothiocyanate-forming system is therefore supposed to afford only a limited effect on the development of gingivitis and paradentosis and a proper inhibition can hardly be obtained thereby.
- An effective inhibition and treatment of gingivitis and paradentosis can solely be obtained by extinguishing the bacteria localized between the teeth and in the gingival pockets. None of the above mentioned methods of inhibiting the formation of plaques either by preventing dextrans from forming or by attenuating or possibly extinguishing the bacteria involved proves any properly substantiated effect on plaques occurring between the teeth and in gingival pockets and the primary object of the inventions referred to actually also consists in the inhibition of caries.
- composition according to the invention containing at least one enzyme which in aqueous milieu and in the presence of oxygen and a substrate for the enzyme is capable of generating hydrogen peroxide and a peroxidase and a thiocyanate, the composition according to the invention being characterized in that it further contains lysozyme.
- the hydrogen peroxide forming enzyme When the bactericidal composition according to the invention, for example in prophylaxis or treatment of gingivitis or paradentosis, is applied in use between the tooth spaces and into the gingival pockets, the hydrogen peroxide forming enzyme will come into contact with food debris and stimulant debris containing substances acting as substrate for the enzyme. This entails oxidation of said substances under the liberation of hydrogen peroxide which by the influence of the peroxidase contained in the composition and naturally available peroxidase is reacted with thiocyanate, thereby generating hypothiocyanate (OSCN-).
- OSCN- hypothiocyanate
- composition according to the invention is characterized in that the hydrogen peroxide forming enzyme is glucose oxidase.
- composition when the composition is to be used in connection with dental hygiene it may be appropriate that it further contains invertase or lactase or both, so that sucrose or lactose present in the oral cavity are decomposed under the formation of glucose serving as substrate for the glucose oxidase.
- invertase or lactase or both sucrose or lactose present in the oral cavity are decomposed under the formation of glucose serving as substrate for the glucose oxidase.
- the composition may in itself contain a substrate, also when used for dental hygiene, in which case the formation of hydrogen peroxide is not restricted by the amount of substances naturally present in the oral cavity and which act as substrate for the hydrogen peroxide forming enzyme.
- a composition according to the invention which contains glucose oxidase is particularly intended to be used for wound treatment, it is expedient that it further includes glucose or glucose forming combinations of materials as substrate for the glucose oxidase and has a water content not exceeding the lowest value at which the glucose oxidase is active.
- the reactions resulting in the generation of hypothiocyanate attenuating bacteria and causing them to be extinguished by the lysozyme occur only when the composition comes into contact with water, e.g. in the form of blood or lymph and, consequently, the composition is stable until use.
- the composition may as well contain no glucose, but if so, it is used together with a glucose- containing or forming agent.
- the alternatively hydrogen peroxide forming enzyme may be an amino acid oxidase, in which case the amino acida present in blood and lymph act as substrate.
- the peroxidase constituting a component of the composition and ensuring the formation of hypothiocyanate from the thiocyanate and the hydrogen peroxide is lactoperoxidase, myeloperoxidase or horseradish peroxidase, all of which enzymes are classified E.C.1.17.1.7 (IUPAC).
- the invention further relates to dental hygiene preparations that are particularly suitable to ensure a selectively high mortality of bacteria in tooth spaces and in the gingival pockets without affecting the microflora in the oral cavity.
- a general affection on the total microflora in the oral cavity is incidentally undesired.
- the dental hygiene agents according to the invention may be in the form of toothpicks, tooth thread of miniature brushes, in particular for the prevention and treatment of gingivitis and paradentosis and are characterized in that they are impregnated with the above mentioned composition according to the invention.
- the impregnation of for example tooth picks may be effected by humidifying them with solutions of the hydrogen peroxide forming enzyme, peroxidase, thiocyanate and lysozyme and stabilizing agents known per se for enzymes followed by drying, for instance freeze drying, of the humidified tooth picks.
- the dentifrice may be in the form of a solution or suspension adapted to be introduced into the spaces between the teeth and gingival pockets by means of a brush, tooth thread, toothpicks or the like, said solution containing the hydrogen peroxide generating enzyme, peroxidase, thiocyanate and lysozyme, preferably together with ordinary stabilizing and preservating agents.
- the bactericidal effect of the composition according to the invention is so high that the comparatively small amount of the components of the composition that may be added by means of impregnated toothpicks will be sufficient to thereby obtain a markable bactericidal effect.
- the composition by treatment or prophylaxis of gingivitis and paradentosis for example by means of toothpicks is applied locally into gingival pockets and the spaces between the teeth, this composition may advantageously also be used in other dental hygiene agents, such as toothpaste the use of which only affords an inferior localized supply.
- the composition according to the invention will obvious also be applicable in combination with other agents for enhancing the intended effect.
- the EDTA will be capable of enhancing the effect of the composition on bacteria having a content of calcium conpounds in the cell walls.
- the bactericidal composition is to be used for wound treatment a dressing material may be impregnated with it, ot the composition may constitute a component of a vulneary powder.
- the composition is principally generally applicable else- where, it an effective bactericidal activity is desired which is gentle to the host organism and has a limited duration and which does not involve the same risk of forming resistant strains of micro organisms as when using antibiotics.
- the composition according to the invention may be prepared by use of such enzymes, all of which are approved for use in foodstuffs.
- the quantitaive ratios between the components of the bactericidal composition concerned may vary within a wide range.
- Lactase ⁇ -galactosidase (E.C. 3.2.1.23) is given in the unit L.U. (Lactase unit) defined as the amount of enzyme producing in one minute 1 mg glucose from 6 ml of 5.0% lactose at 20°C and pH 6.5.
- Invertase ⁇ -fructofuranosidase (E.C. 3.2.1.26) is given in S.U. (Sumner Unit) defined as the amount of enzyme generating in five minutes 1 mg invert sugar from 6 ml of 5 .4% sucrose at 20°C and pH 4.7.
- Glucose oxidase is classified E.C. 1.1.3.4.
- Lactoperoxidase is classified E.C. 1.17.1.7. and has the function in the composition concerned to catalyze the reaction H 2 O 2 + SCN ⁇ H 2 O
- Lysozyme also designated muramidase, is N-acetylmura- mylhydrolase (E.C. 3.2.1.17). This enzyme decomposes N-acethylglucosamine and N-acetylmuramic acid from the cell walls.
- the activity of lysozyme is measured by lysing of Micrococcus lysodieticus. As a well defined standard of the activity is difficult to determine, the amount of the lysozyme applied is, however, given in absolute values in the following examples.
- the material as used had a purity of 99% and was extracted from egg white.
- the bacterial cultures were subsequently incubated for three days at temperatures of 25, 37, 40 or 45°C, during which intervals the cultures were daily inspected. It turned out that in every case the baterial growth ceased even with an addition of only 50 ⁇ l of the solution per 10 ml growth culture.
- the toothpicks were subsequently freeze-dried. With a view to testing the bactericidal effect of the toothpicks thus impregnated, 10 5 bacteria of different strains of the genera Streptococcus and Lactobacillus were incubated on agar plates.
- the tooth picks that were impegnated with the composition according to the invention were pressed down into the sucrose- containing agar plates which were then incubated for six days, and the growth was daily observed. Tests were carried out by incubation at 25°C as well as at 40°C, and in particular at 37°C. A clear zone had formed around the toothpicks, completely free of bacterial growth in an area varying between 5 and 15 mm reckoned from the toothpick.
- toothpicks were impregnated with an aqueous buffer solution of lysozyme and EDTA in the above quoted amounts but without enzymes and NaSCN. Said latter toothpicks could not prove any significant bactericidal actvity by the test carried out.
- Distilled water ad 100 ml It can be calculated that in use such a solution will be diluted about three times by saliva available in gingival pockets and on the surfaces of the tooth necks. The resulting concentration of the components contained in the composition to about one fourth of the above specified values will be completely sufficient to obtain the desired bacteriostatic activity.
- Example 5 An aqueous solution as in Example 5 was prepared but without invertase and colour and flavourings. Gauze wads were drenched with the solution and subsequently dried in vacuum. The resulting wound dressing material was suitable to be used in connection with a vulnerable powder containing besides the components ordinary of such a powder, about 2% by weight of glucose.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NO882708A NO882708L (no) | 1986-10-20 | 1988-06-17 | Enzymholdig baktericid blanding og tann- og saarbehandlingspreparater som omfatter blandingen. |
FI882947A FI882947A (fi) | 1986-10-20 | 1988-06-20 | Enzyminnehaollande bakteriedoedande komposition och vaordpreparat foer taender och saor innehaollande denna komposition. |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK501686A DK501686A (da) | 1986-10-20 | 1986-10-20 | Enzymholdigt, baktericidt middel samt tandplejemidler og saarbehandlingsmidler, som indeholder det |
DK5016/86 | 1986-10-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1988002600A1 true WO1988002600A1 (fr) | 1988-04-21 |
Family
ID=8138685
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK1987/000130 WO1988002600A1 (fr) | 1986-10-20 | 1987-10-19 | Composition bactericide contenant une enzyme et preparation pour le traitement d'infections notamment dentaires comprenant ladite composition |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0293407A1 (fr) |
JP (1) | JPH01501000A (fr) |
AU (1) | AU8175087A (fr) |
DK (1) | DK501686A (fr) |
FI (1) | FI882947A (fr) |
WO (1) | WO1988002600A1 (fr) |
Cited By (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2651433A1 (fr) * | 1989-09-07 | 1991-03-08 | Dana Dominique | Complexe enzymatique a activite sur le tartre et la carie dentaire. |
WO1991006217A1 (fr) * | 1989-10-27 | 1991-05-16 | Genencor International Europe Oy | Procede de lutte contre les microorganismes |
EP0461530A2 (fr) * | 1990-06-13 | 1991-12-18 | HAARMANN & REIMER CORP. | Compositions antimicrobiennes synergiques |
WO1992010165A1 (fr) * | 1990-12-05 | 1992-06-25 | Warner-Lambert Company | Solution nettoyante pour dentiers, contenant des enzymes |
EP0500387A2 (fr) * | 1991-02-21 | 1992-08-26 | Exoxemis, Inc. | Méthodes et compositions pour le traitement des infections et le contrôle de la flore utilisant l'haloperoxydase |
FR2685202A1 (fr) * | 1991-12-24 | 1993-06-25 | Sederma Sa | Nouvelle methode de traitement pharmaceutique et cosmetique pour la regulation de la seborrhee, de l'acne et de la flore cutanee. |
US5250299A (en) * | 1991-09-23 | 1993-10-05 | Haarmann & Reimer Corp. | Synergistic antimicrobial compositions |
FR2695034A1 (fr) * | 1992-09-01 | 1994-03-04 | Oreal | Composition cosmétique ou pharmaceutique comprenant en association une peroxydase et un agent anti-oxygène singulet. |
US5389369A (en) * | 1991-02-21 | 1995-02-14 | Exoxemis, Inc. | Halo peroxidase containing compositions for killing yeast and sporular microorganisms |
WO1995014784A1 (fr) * | 1993-11-24 | 1995-06-01 | Monsanto Company | Procede de limitation d'agents pathogenes chez les vegetaux |
WO1996015682A1 (fr) * | 1994-11-22 | 1996-05-30 | Ing. Erich Erber Kommanditgesellschaft | Additif pour produits de fourrage et pour eau potable permettant de renforcer la resistance au stress et l'immunite d'animaux de rapport |
US5565197A (en) * | 1991-02-21 | 1996-10-15 | Exoxemis, Inc. | Method which utilizes a haloperoxidase composition to inhibit the growth of microorganisms which cause sexually transmitted diseases |
US5607681A (en) * | 1990-02-03 | 1997-03-04 | The Boots Company Plc | Anti-microbial compositions |
WO1997026908A1 (fr) * | 1996-01-23 | 1997-07-31 | Semper Aktiebolag | Utilisation de lactoperoxydase, d'un donneur de peroxyde et de thiocyanate pour la preparation d'un medicament permettant de soigner une infection par helicobacter pylori |
US5756090A (en) * | 1991-02-21 | 1998-05-26 | Eoe, Inc. | Oxygen activatable formulations for disinfection or sterilization |
WO1998049272A2 (fr) * | 1997-04-29 | 1998-11-05 | Knoll Aktiengesellschaft | Concentre enzymatique |
US5888505A (en) * | 1991-02-21 | 1999-03-30 | Eoe, Inc. | Method for selectively inhibiting the growth of microbes using a haloperoxidase-halide-peroxide system |
FR2777785A1 (fr) * | 1998-04-27 | 1999-10-29 | Sederma Sa | Utilisation de l'association peroxydase de raifort/acide cafeique dans des compositions cosmetiques ou dermopharma- ceutiques destinees a prevenir et/ou corriger les degats cutanes induits par les formes radicalaires de l'oxygene |
EP1010430A1 (fr) * | 1998-04-30 | 2000-06-21 | Snow Brand Milk Products, Co., Ltd. | Substances a action preventive et agents d'amelioration de la parodontose |
FR2803199A1 (fr) * | 1999-12-29 | 2001-07-06 | Jean Dominique Dana | Procede de transformation des sucres alimentaires cariogenes en produits neutres acariogenes ou cariostatiques et composition pour la mise en oeuvre |
EP0994716B2 (fr) † | 1997-07-25 | 2007-07-04 | Westgate Biological Limited | Secretions immunes maternelles et leur utilisation dans le traitement et/ou la prophylaxie d'etats du corps humain |
WO2008041218A1 (fr) * | 2006-10-06 | 2008-04-10 | Institute Of Technology Sligo | Système antimicrobien et immunostimulateur comprenant une enzyme oxydoréductase |
EP2073836A2 (fr) * | 2006-10-10 | 2009-07-01 | Laclede, Inc. | Procédés et compositions pour le traitement de maladies vaginales utilisant des enzymes produisant du peroxyde et des peroxydases |
WO2014191995A2 (fr) | 2013-05-27 | 2014-12-04 | Rakuto Bio Technologies Ltd. | Compositions cosmétiques contenant un système enzymatique |
US8933147B2 (en) | 2005-11-17 | 2015-01-13 | 3M Innovative Properties Company | Anti-microbial dental impression material |
US8945540B2 (en) | 2008-05-09 | 2015-02-03 | Exoxemis, Inc. | Compositions for enhancing the antibacterial activity of myeloperoxidase and methods of use thereof |
KR101498510B1 (ko) * | 2012-06-26 | 2015-03-04 | 서울대학교산학협력단 | 히알루론산을 포함하는 인공 타액 조성물 |
FR3020758A1 (fr) * | 2014-05-12 | 2015-11-13 | Jean-Dominique Dana | Composition pour ameliorer l'hygiene buco-dentaire |
US9393249B2 (en) | 2008-03-26 | 2016-07-19 | Institute Of Technology Sligo | Antimicrobial composition |
US9522165B2 (en) | 2006-10-06 | 2016-12-20 | Institute Of Technology Sligo | Formulation and method for the treatment of fungal nail infections |
EP3192498A1 (fr) * | 2016-01-12 | 2017-07-19 | Unilever PLC | Compositions de protection orale |
EP3192495A1 (fr) * | 2016-01-12 | 2017-07-19 | Unilever PLC | Compositions de protection orale |
EP3192494A1 (fr) * | 2016-01-12 | 2017-07-19 | Unilever PLC | Compositions de protection orale |
US9782459B2 (en) | 2011-07-11 | 2017-10-10 | Exoxemis, Inc. | Eosinophil peroxidase compositions and methods of their use |
EP3297442A4 (fr) * | 2015-05-18 | 2018-11-07 | Zymtronix, LLC | Enzymes microbicides immobilisés magnétiquement |
US10260061B2 (en) | 2011-03-10 | 2019-04-16 | Cornell University | Mesoporous catalysts of magnetic nanoparticles and free-radical-producing enzymes, and methods of use |
US10351841B2 (en) | 2012-10-05 | 2019-07-16 | Cornell University | Method for inhibiting growth of microorganisms with macroporous scaffolds |
EP3603654A1 (fr) * | 2014-11-24 | 2020-02-05 | Matoke Holdings Limited | Prévention et traitement d'infections microbiennes |
US10792649B2 (en) | 2015-07-15 | 2020-10-06 | Zymtronix, Llc | Automated bionanocatalyst production |
US10993436B2 (en) | 2016-08-13 | 2021-05-04 | Zymtronix Catalytic Systems, Inc. | Magnetically immobilized biocidal enzymes and biocidal chemicals |
CN113317327A (zh) * | 2020-02-28 | 2021-08-31 | 中粮营养健康研究院有限公司 | 一种杀菌杀病毒酶制剂组合物及其应用 |
US11185080B2 (en) | 2014-04-30 | 2021-11-30 | Matoke Holdings Limited | Antimicrobial compositions |
CN115633706A (zh) * | 2022-09-27 | 2023-01-24 | 深圳市减化生物科技有限公司 | 一种生物杀菌除霉剂及其制备方法、清洁用品 |
US11730168B2 (en) | 2017-10-16 | 2023-08-22 | Matoke Holdings Limited | Antimicrobial superabsorbent compositions |
EP3192564B1 (fr) * | 2016-01-12 | 2024-07-10 | Unilever IP Holdings B.V. | Composition de protection orale |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2860154B1 (fr) * | 2003-09-29 | 2006-02-03 | Chris Cardon | Composition pour le traitement de la mauvaise haleine |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2852792A1 (de) * | 1977-12-06 | 1979-06-07 | Anic Spa | Karies-verhuetende zahnreinigungsmittel-zusammensetzung |
DE2937964A1 (de) * | 1979-09-20 | 1981-03-26 | Peter 3400 Göttingen Schilling | Mittel zur bekaempfung der karies |
EP0133736A2 (fr) * | 1983-06-06 | 1985-03-06 | Laclede Professional Products, Inc. | Dentifrice di-enzymatique |
-
1986
- 1986-10-20 DK DK501686A patent/DK501686A/da not_active Application Discontinuation
-
1987
- 1987-10-19 EP EP87907213A patent/EP0293407A1/fr not_active Withdrawn
- 1987-10-19 AU AU81750/87A patent/AU8175087A/en not_active Abandoned
- 1987-10-19 WO PCT/DK1987/000130 patent/WO1988002600A1/fr not_active Application Discontinuation
- 1987-10-19 JP JP62506623A patent/JPH01501000A/ja active Pending
-
1988
- 1988-06-20 FI FI882947A patent/FI882947A/fi not_active IP Right Cessation
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2852792A1 (de) * | 1977-12-06 | 1979-06-07 | Anic Spa | Karies-verhuetende zahnreinigungsmittel-zusammensetzung |
DE2937964A1 (de) * | 1979-09-20 | 1981-03-26 | Peter 3400 Göttingen Schilling | Mittel zur bekaempfung der karies |
EP0133736A2 (fr) * | 1983-06-06 | 1985-03-06 | Laclede Professional Products, Inc. | Dentifrice di-enzymatique |
Non-Patent Citations (1)
Title |
---|
CHEMICAL ABSTRACTS, Vol 86 (1977), Abstract No 127293d, USSR 540637 * |
Cited By (76)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2651433A1 (fr) * | 1989-09-07 | 1991-03-08 | Dana Dominique | Complexe enzymatique a activite sur le tartre et la carie dentaire. |
WO1991006217A1 (fr) * | 1989-10-27 | 1991-05-16 | Genencor International Europe Oy | Procede de lutte contre les microorganismes |
US5607681A (en) * | 1990-02-03 | 1997-03-04 | The Boots Company Plc | Anti-microbial compositions |
EP0461530A2 (fr) * | 1990-06-13 | 1991-12-18 | HAARMANN & REIMER CORP. | Compositions antimicrobiennes synergiques |
EP0461530A3 (en) * | 1990-06-13 | 1992-06-03 | Haarmann & Reimer Corp. | Synergistic antimicrobial compositions |
WO1992010165A1 (fr) * | 1990-12-05 | 1992-06-25 | Warner-Lambert Company | Solution nettoyante pour dentiers, contenant des enzymes |
EP0923939A1 (fr) * | 1991-02-21 | 1999-06-23 | Eoe, Inc. | Méthodes et compositions pour le traitement des infections et le contrôle de la flore |
US5718896A (en) * | 1991-02-21 | 1998-02-17 | Eoe, Inc. | Methods for the inhibiting the transmission of sexually transmitted diseases |
US6294168B1 (en) | 1991-02-21 | 2001-09-25 | Exoxemis, Inc. | Method for inhibiting pathogenic microbes in an animal using a haloperoxidase-halide-peroxide system |
US6503507B1 (en) | 1991-02-21 | 2003-01-07 | Exoxemis, Inc. | Oxygen activatable formulations for disinfection or sterilization |
EP0500387A2 (fr) * | 1991-02-21 | 1992-08-26 | Exoxemis, Inc. | Méthodes et compositions pour le traitement des infections et le contrôle de la flore utilisant l'haloperoxydase |
US5389369A (en) * | 1991-02-21 | 1995-02-14 | Exoxemis, Inc. | Halo peroxidase containing compositions for killing yeast and sporular microorganisms |
US6033662A (en) * | 1991-02-21 | 2000-03-07 | Exoxemis, Inc. | Oxygen activatable formulations for disinfection or sterilization |
US5451402A (en) * | 1991-02-21 | 1995-09-19 | Exoxemis, Inc. | Method for killing yeast and sporular microorganisms with haloperoxidase-containing compositions |
US5510104A (en) * | 1991-02-21 | 1996-04-23 | Exoxemis, Inc. | Method for killing or inhabiting the growth of sporulating microorganisms with haloperoxidase-containing compositions |
US5888505A (en) * | 1991-02-21 | 1999-03-30 | Eoe, Inc. | Method for selectively inhibiting the growth of microbes using a haloperoxidase-halide-peroxide system |
US5565197A (en) * | 1991-02-21 | 1996-10-15 | Exoxemis, Inc. | Method which utilizes a haloperoxidase composition to inhibit the growth of microorganisms which cause sexually transmitted diseases |
EP0500387A3 (en) * | 1991-02-21 | 1992-10-28 | Exoxemis, Inc. | Methods and compositions for the treatment of infection and control of flora using haloperoxidase |
US5756090A (en) * | 1991-02-21 | 1998-05-26 | Eoe, Inc. | Oxygen activatable formulations for disinfection or sterilization |
US5250299A (en) * | 1991-09-23 | 1993-10-05 | Haarmann & Reimer Corp. | Synergistic antimicrobial compositions |
FR2685202A1 (fr) * | 1991-12-24 | 1993-06-25 | Sederma Sa | Nouvelle methode de traitement pharmaceutique et cosmetique pour la regulation de la seborrhee, de l'acne et de la flore cutanee. |
FR2695034A1 (fr) * | 1992-09-01 | 1994-03-04 | Oreal | Composition cosmétique ou pharmaceutique comprenant en association une peroxydase et un agent anti-oxygène singulet. |
US6316012B1 (en) | 1992-09-01 | 2001-11-13 | L'oreal | Cosmetic or pharmaceutical composition comprising, in combination, a peroxidase and an anti-singlet oxygen agent |
EP0586303A1 (fr) * | 1992-09-01 | 1994-03-09 | L'oreal | Composition cosmétique ou pharmaceutique comprenant en association une peroxydase et un agent anti-oxygène singulet |
WO1995014784A1 (fr) * | 1993-11-24 | 1995-06-01 | Monsanto Company | Procede de limitation d'agents pathogenes chez les vegetaux |
WO1996015682A1 (fr) * | 1994-11-22 | 1996-05-30 | Ing. Erich Erber Kommanditgesellschaft | Additif pour produits de fourrage et pour eau potable permettant de renforcer la resistance au stress et l'immunite d'animaux de rapport |
WO1997026908A1 (fr) * | 1996-01-23 | 1997-07-31 | Semper Aktiebolag | Utilisation de lactoperoxydase, d'un donneur de peroxyde et de thiocyanate pour la preparation d'un medicament permettant de soigner une infection par helicobacter pylori |
US6149908A (en) * | 1996-01-23 | 2000-11-21 | Semper Ab | Use of lactoperoxidase, a peroxide donor and thiocyanate for the manufacture of a medicament for treating Helicobacter pylori infection |
WO1998049272A2 (fr) * | 1997-04-29 | 1998-11-05 | Knoll Aktiengesellschaft | Concentre enzymatique |
WO1998049272A3 (fr) * | 1997-04-29 | 1999-02-04 | Knoll Ag | Concentre enzymatique |
US6312687B1 (en) | 1997-04-29 | 2001-11-06 | Knoll Aktiengesellschaft | Stabilized lactoperoxidase and glucose oxidase concentrate |
EP0994716B2 (fr) † | 1997-07-25 | 2007-07-04 | Westgate Biological Limited | Secretions immunes maternelles et leur utilisation dans le traitement et/ou la prophylaxie d'etats du corps humain |
WO1999055301A1 (fr) * | 1998-04-27 | 1999-11-04 | Sederma | Utilisation de la peroxydase de raifort dans des compositions cosmetiques ou dermopharmaceutiques destinees a prevenir et/ou corriger les degats cutanes induits par les formes radicalaires de l'oxygene |
FR2777785A1 (fr) * | 1998-04-27 | 1999-10-29 | Sederma Sa | Utilisation de l'association peroxydase de raifort/acide cafeique dans des compositions cosmetiques ou dermopharma- ceutiques destinees a prevenir et/ou corriger les degats cutanes induits par les formes radicalaires de l'oxygene |
EP1010430A4 (fr) * | 1998-04-30 | 2004-03-31 | Snow Brand Milk Products Co Ltd | Substances a action preventive et agents d'amelioration de la parodontose |
EP1010430A1 (fr) * | 1998-04-30 | 2000-06-21 | Snow Brand Milk Products, Co., Ltd. | Substances a action preventive et agents d'amelioration de la parodontose |
WO2001049241A2 (fr) * | 1999-12-29 | 2001-07-12 | Dana Jean Dominique | Composition d'hygiene bucco-dentaire |
FR2803199A1 (fr) * | 1999-12-29 | 2001-07-06 | Jean Dominique Dana | Procede de transformation des sucres alimentaires cariogenes en produits neutres acariogenes ou cariostatiques et composition pour la mise en oeuvre |
US6716611B2 (en) | 1999-12-29 | 2004-04-06 | Jean Dominique Dana | Method for transforming cariogenic food sugars into acariogenic or cariostatic neutral products and composition therefor |
WO2001049241A3 (fr) * | 1999-12-29 | 2001-12-20 | Dana Jean Dominique | Composition d'hygiene bucco-dentaire |
US8933147B2 (en) | 2005-11-17 | 2015-01-13 | 3M Innovative Properties Company | Anti-microbial dental impression material |
WO2008041218A1 (fr) * | 2006-10-06 | 2008-04-10 | Institute Of Technology Sligo | Système antimicrobien et immunostimulateur comprenant une enzyme oxydoréductase |
US9522165B2 (en) | 2006-10-06 | 2016-12-20 | Institute Of Technology Sligo | Formulation and method for the treatment of fungal nail infections |
US9522177B2 (en) | 2006-10-06 | 2016-12-20 | Institute Of Technology Sligo | Antimicrobial and immunostimulatory system comprising an oxidoreductase enzyme |
AU2007307921B2 (en) * | 2006-10-10 | 2013-03-28 | Laclede, Inc. | Methods and compositions for the treatment of vaginal diseases employing peroxide-producing enzymes and peroxidases |
EP2073836A4 (fr) * | 2006-10-10 | 2010-04-07 | Laclede Inc | Procédés et compositions pour le traitement de maladies vaginales utilisant des enzymes produisant du peroxyde et des peroxydases |
EP2073836A2 (fr) * | 2006-10-10 | 2009-07-01 | Laclede, Inc. | Procédés et compositions pour le traitement de maladies vaginales utilisant des enzymes produisant du peroxyde et des peroxydases |
US9393249B2 (en) | 2008-03-26 | 2016-07-19 | Institute Of Technology Sligo | Antimicrobial composition |
US8945540B2 (en) | 2008-05-09 | 2015-02-03 | Exoxemis, Inc. | Compositions for enhancing the antibacterial activity of myeloperoxidase and methods of use thereof |
US10316313B2 (en) | 2011-03-10 | 2019-06-11 | Cornell University | Mesoporous catalysts of magnetic nanoparticles and free-radical-producing enzymes, and methods of use |
US10260061B2 (en) | 2011-03-10 | 2019-04-16 | Cornell University | Mesoporous catalysts of magnetic nanoparticles and free-radical-producing enzymes, and methods of use |
US9782459B2 (en) | 2011-07-11 | 2017-10-10 | Exoxemis, Inc. | Eosinophil peroxidase compositions and methods of their use |
KR101498510B1 (ko) * | 2012-06-26 | 2015-03-04 | 서울대학교산학협력단 | 히알루론산을 포함하는 인공 타액 조성물 |
US11236322B2 (en) | 2012-10-05 | 2022-02-01 | Cornell University | Enzyme forming mesoporous assemblies embedded in macroporous scaffolds |
US10351841B2 (en) | 2012-10-05 | 2019-07-16 | Cornell University | Method for inhibiting growth of microorganisms with macroporous scaffolds |
US10767172B2 (en) | 2012-10-05 | 2020-09-08 | Cornell University | Method for epoxidation to produce alkene oxide |
US10980730B2 (en) | 2013-05-27 | 2021-04-20 | Rakuto Bio Technologies Ltd. | Enzymatic system-containing cosmetic compositions |
WO2014191995A2 (fr) | 2013-05-27 | 2014-12-04 | Rakuto Bio Technologies Ltd. | Compositions cosmétiques contenant un système enzymatique |
EP3626310A1 (fr) | 2013-05-27 | 2020-03-25 | Rakuto Bio Technologies Ltd. | Système enzymatique contenant des compositions cosmétiques |
US11311017B2 (en) | 2014-04-30 | 2022-04-26 | Matoke Holdings Limited | Antimicrobial compositions |
US11185080B2 (en) | 2014-04-30 | 2021-11-30 | Matoke Holdings Limited | Antimicrobial compositions |
WO2015173022A1 (fr) * | 2014-05-12 | 2015-11-19 | Jean-Dominique Dana | Composition pour ameliorer l'hygiene bucco-dentaire |
FR3020758A1 (fr) * | 2014-05-12 | 2015-11-13 | Jean-Dominique Dana | Composition pour ameliorer l'hygiene buco-dentaire |
EP3603654A1 (fr) * | 2014-11-24 | 2020-02-05 | Matoke Holdings Limited | Prévention et traitement d'infections microbiennes |
US10881102B2 (en) | 2015-05-18 | 2021-01-05 | Zymtronix, Llc | Magnetically immobilized microbiocidal enzymes |
EP3297442A4 (fr) * | 2015-05-18 | 2018-11-07 | Zymtronix, LLC | Enzymes microbicides immobilisés magnétiquement |
US11517014B2 (en) | 2015-05-18 | 2022-12-06 | Zymtronix, Inc. | Magnetically immobilized microbiocidal enzymes |
US10792649B2 (en) | 2015-07-15 | 2020-10-06 | Zymtronix, Llc | Automated bionanocatalyst production |
EP3192494A1 (fr) * | 2016-01-12 | 2017-07-19 | Unilever PLC | Compositions de protection orale |
EP3192495A1 (fr) * | 2016-01-12 | 2017-07-19 | Unilever PLC | Compositions de protection orale |
EP3192498A1 (fr) * | 2016-01-12 | 2017-07-19 | Unilever PLC | Compositions de protection orale |
EP3192564B1 (fr) * | 2016-01-12 | 2024-07-10 | Unilever IP Holdings B.V. | Composition de protection orale |
US10993436B2 (en) | 2016-08-13 | 2021-05-04 | Zymtronix Catalytic Systems, Inc. | Magnetically immobilized biocidal enzymes and biocidal chemicals |
US11730168B2 (en) | 2017-10-16 | 2023-08-22 | Matoke Holdings Limited | Antimicrobial superabsorbent compositions |
CN113317327A (zh) * | 2020-02-28 | 2021-08-31 | 中粮营养健康研究院有限公司 | 一种杀菌杀病毒酶制剂组合物及其应用 |
CN115633706A (zh) * | 2022-09-27 | 2023-01-24 | 深圳市减化生物科技有限公司 | 一种生物杀菌除霉剂及其制备方法、清洁用品 |
Also Published As
Publication number | Publication date |
---|---|
AU8175087A (en) | 1988-05-06 |
DK501686A (da) | 1988-04-21 |
FI882947A0 (fi) | 1988-06-20 |
EP0293407A1 (fr) | 1988-12-07 |
DK501686D0 (da) | 1986-10-20 |
JPH01501000A (ja) | 1989-04-06 |
FI882947A (fi) | 1988-06-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO1988002600A1 (fr) | Composition bactericide contenant une enzyme et preparation pour le traitement d'infections notamment dentaires comprenant ladite composition | |
US4152418A (en) | Zinc and enzyme mouthwash and mouthwash concentrate for reducing dental plaque and calculus formation | |
US4082841A (en) | Dentifrice | |
US4150113A (en) | Enzymatic dentifrices | |
US4537764A (en) | Stabilized enzymatic dentifrice containing B-D-glucose and glucose oxidase | |
US4564519A (en) | Di-enzymatic chewable dentifrice | |
US5908614A (en) | Peroxidase-activating oral compositions | |
US4269822A (en) | Antiseptic dentifrice | |
US4178362A (en) | Enzymatic dentifrices | |
US4837009A (en) | Method and composition for prevention of plaque formation and plaque dependent diseases | |
US5200171A (en) | Oral health preparation and method | |
KR100613543B1 (ko) | 세정제용 살균제 | |
US4818519A (en) | Method and composition for prevention of plaque formation and plaque dependent diseases | |
US5348734A (en) | Oral health preparation and method | |
US5262151A (en) | Stabilized enzymatic antimicrobial compositions | |
EP0907349B1 (fr) | Compositions permettant l'elimination de la plaque dentaire | |
CA2496800C (fr) | Enzyme anti-plaque contenant une composition a deux composants | |
AU769345B2 (en) | Antimicrobial denture cleansing compositions | |
MXPA04011150A (es) | Dentifrico antibacterial que exhibe propiedades en contra de la placa y para refrescar el aliento mejoradas. | |
FI87423C (fi) | Tandvaordsprodukt | |
WO1999020239A1 (fr) | Compositions orales inhibant la plaque dentaire | |
NO882708L (no) | Enzymholdig baktericid blanding og tann- og saarbehandlingspreparater som omfatter blandingen. | |
Frostell | Plaque metabolism and substrate concentration | |
JPH0812544A (ja) | 口腔用組成物 | |
WO1997038670A1 (fr) | Compositions permettant l'elimination de la plaque dentaire |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AU FI JP NO US |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): AT BE CH DE FR GB IT LU NL SE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1987907213 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 882947 Country of ref document: FI |
|
WWP | Wipo information: published in national office |
Ref document number: 1987907213 Country of ref document: EP |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1987907213 Country of ref document: EP |