WO1982003079A1 - Synthese rapide en phase solide d'oligonucleotides par l'activation d'oxychlorure de phosphore - Google Patents

Synthese rapide en phase solide d'oligonucleotides par l'activation d'oxychlorure de phosphore Download PDF

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Publication number
WO1982003079A1
WO1982003079A1 PCT/US1982/000305 US8200305W WO8203079A1 WO 1982003079 A1 WO1982003079 A1 WO 1982003079A1 US 8200305 W US8200305 W US 8200305W WO 8203079 A1 WO8203079 A1 WO 8203079A1
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WO
WIPO (PCT)
Prior art keywords
nucieoside
support
reactor
synthesis
oligonucleotides
Prior art date
Application number
PCT/US1982/000305
Other languages
English (en)
Inventor
Inc Bioresearch
Michael S Verlander
William Dean Fuller
Murray Goodman
Original Assignee
Inc Bioresearch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inc Bioresearch filed Critical Inc Bioresearch
Priority to AU83321/82A priority Critical patent/AU8332182A/en
Publication of WO1982003079A1 publication Critical patent/WO1982003079A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

Procede de synthese d'oligonucleotides comprenant les etapes consistant a remplir un reacteur a ecoulement avec un nucleoside supporte se composant d'un premier nucleoside attache en position (5') a un support solide insoluble, a activer ce nucleoside de support par un traitement a l'oxychlorure de phosphore en presence d'un catalyseur basique afin de former un groupe 3'-dichlorophosphoryle sur le premier nucleoside, a coupler un deuxieme nucleoside analogue ou different, non protege aux positions 3'et 5', au premier nucleoside par condensation avec le groupe dichlorophosphoryle et a repeter cette etape d'activation et de couplage jusqu'a obtention de la sequence d'oligonucleotide desires. L'oligonucleotide peut ensuite etre extrait par clivage du support et purifie, si necessaire.
PCT/US1982/000305 1981-03-10 1982-03-09 Synthese rapide en phase solide d'oligonucleotides par l'activation d'oxychlorure de phosphore WO1982003079A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU83321/82A AU8332182A (en) 1981-03-10 1982-03-09 Rapid solid phase synthesis of oligonucleotides using phosphorus oxychloride activation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24223181A 1981-03-10 1981-03-10
US242231810310 1981-03-10

Publications (1)

Publication Number Publication Date
WO1982003079A1 true WO1982003079A1 (fr) 1982-09-16

Family

ID=22913966

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1982/000305 WO1982003079A1 (fr) 1981-03-10 1982-03-09 Synthese rapide en phase solide d'oligonucleotides par l'activation d'oxychlorure de phosphore

Country Status (3)

Country Link
EP (1) EP0073826A1 (fr)
WO (1) WO1982003079A1 (fr)
ZA (1) ZA821534B (fr)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4507433A (en) * 1983-10-07 1985-03-26 The Johns Hopkins University Preparation of oligodeoxyribonucleoside alkyl or arylphosphonates
US4742163A (en) * 1985-12-18 1988-05-03 Senju Pharmaceutical Co., Ltd. Alpha-tocopherol (halo)uridine phosphoric acid diester, salts thereof, and methods for producing the same
EP0375278A2 (fr) * 1988-12-21 1990-06-27 The Perkin-Elmer Corporation Système automatique pour la synthèse et purification des polynucléotides
WO1994001446A2 (fr) * 1992-07-09 1994-01-20 Beckman Instruments, Inc. Support solide organique derive utilise pour effectuer la synthese d'acides nucleiques
US5539097A (en) * 1983-09-02 1996-07-23 Molecular Biosystems, Inc. Oligonucleotide polymeric support system
AU693487B2 (en) * 1993-12-16 1998-07-02 Sigma-Aldrich Co. Llc Process for preparing polynucleotides on a solid support and apparatus permitting its implementation
US5935527A (en) * 1988-12-21 1999-08-10 The Perkin-Elmer Corporation Automated system for polynucleotide synthesis and purification
US6469157B1 (en) 1993-12-16 2002-10-22 Proligo Llc Process for preparing polynucleotides on a solid support

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3288780A (en) * 1962-01-30 1966-11-29 Ajinomoto Kk Process for preparing 5'-ribonucleotides
US3407190A (en) * 1965-07-27 1968-10-22 Takeda Chemical Industries Ltd Method for the production of 5'-ribonucleotide
US3466273A (en) * 1966-12-16 1969-09-09 Asahi Chemical Ind Process for producing nucleotide by a selective phosphorylation
EP0035719A2 (fr) * 1980-02-29 1981-09-16 University Patents, Inc. Méthode de fabrication de polymères inorganiques modifiés, leur emploi dans des méthodes de production de polynucléeotides et un réactif utilisé dans ces méthodes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3288780A (en) * 1962-01-30 1966-11-29 Ajinomoto Kk Process for preparing 5'-ribonucleotides
US3407190A (en) * 1965-07-27 1968-10-22 Takeda Chemical Industries Ltd Method for the production of 5'-ribonucleotide
US3466273A (en) * 1966-12-16 1969-09-09 Asahi Chemical Ind Process for producing nucleotide by a selective phosphorylation
EP0035719A2 (fr) * 1980-02-29 1981-09-16 University Patents, Inc. Méthode de fabrication de polymères inorganiques modifiés, leur emploi dans des méthodes de production de polynucléeotides et un réactif utilisé dans ces méthodes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Tetrahedron Letters, Volume 21, issued 1980 (Great Britain), MATTEUCCI and CARUTHERS, "The Synthesis of Oligodeoxypyrimidines On A Polymer Support", see pages 719-722. *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5539097A (en) * 1983-09-02 1996-07-23 Molecular Biosystems, Inc. Oligonucleotide polymeric support system
US4507433A (en) * 1983-10-07 1985-03-26 The Johns Hopkins University Preparation of oligodeoxyribonucleoside alkyl or arylphosphonates
US4742163A (en) * 1985-12-18 1988-05-03 Senju Pharmaceutical Co., Ltd. Alpha-tocopherol (halo)uridine phosphoric acid diester, salts thereof, and methods for producing the same
EP0375278A3 (fr) * 1988-12-21 1991-04-10 The Perkin-Elmer Corporation Système automatique pour la synthèse et purification des polynucléotides
EP0375278A2 (fr) * 1988-12-21 1990-06-27 The Perkin-Elmer Corporation Système automatique pour la synthèse et purification des polynucléotides
US5935527A (en) * 1988-12-21 1999-08-10 The Perkin-Elmer Corporation Automated system for polynucleotide synthesis and purification
US6175006B1 (en) 1988-12-21 2001-01-16 The Perkin-Elmer Corporation Automated system for polynucleotide synthesis and purification
WO1994001446A2 (fr) * 1992-07-09 1994-01-20 Beckman Instruments, Inc. Support solide organique derive utilise pour effectuer la synthese d'acides nucleiques
WO1994001446A3 (fr) * 1992-07-09 1994-03-03 Beckman Instruments Inc Support solide organique derive utilise pour effectuer la synthese d'acides nucleiques
AU693487B2 (en) * 1993-12-16 1998-07-02 Sigma-Aldrich Co. Llc Process for preparing polynucleotides on a solid support and apparatus permitting its implementation
US6469157B1 (en) 1993-12-16 2002-10-22 Proligo Llc Process for preparing polynucleotides on a solid support
US6623703B1 (en) 1993-12-16 2003-09-23 Proligo, Llc Process for preparing polynucleotides on a solid support and apparatus permitting its implementation
US6825339B2 (en) 1993-12-16 2004-11-30 Proligo, Llc Apparatus for preparing polynucleotides on a solid support

Also Published As

Publication number Publication date
EP0073826A1 (fr) 1983-03-16
ZA821534B (en) 1984-04-25

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