WO1994001446A3 - Support solide organique derive utilise pour effectuer la synthese d'acides nucleiques - Google Patents

Support solide organique derive utilise pour effectuer la synthese d'acides nucleiques Download PDF

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Publication number
WO1994001446A3
WO1994001446A3 PCT/US1993/006214 US9306214W WO9401446A3 WO 1994001446 A3 WO1994001446 A3 WO 1994001446A3 US 9306214 W US9306214 W US 9306214W WO 9401446 A3 WO9401446 A3 WO 9401446A3
Authority
WO
WIPO (PCT)
Prior art keywords
linker
nucleoside
nucleic acid
solid support
polymer
Prior art date
Application number
PCT/US1993/006214
Other languages
English (en)
Other versions
WO1994001446A2 (fr
Inventor
Parameswara M Reddy
Maged A Michael
Original Assignee
Beckman Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Publication of WO1994001446A2 publication Critical patent/WO1994001446A2/fr
Publication of WO1994001446A3 publication Critical patent/WO1994001446A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

Intermédiaire de synthèse nucléotidique en phase solide utile pour effectuer la synthèse chimique d'oligodésoxyribonucléotides et d'oligonucléotides comprenant: (1) un support particulaire constitué d'un polymère poreux dont le squelette comprend des fractions acrylate ou méthacrylate facultativement substituées; (2) un nucléoside; et (3) un segment de liaison comportant des première et deuxième extrémités qui sont respectivement liées de manière covalente au support particulaire et au nucléoside, le segment de liaison éloignant le nucléoside à au moins trois atomes du polymère. Ledit segment de liaison peut comporter au moins une diamine aliphatique facultativement substituée ou bien il peut comprendre une fraction de polyéthylène glycol. Le polymère poreux est de préférence un polymère méthacrylate-vinylidène. On peut utiliser le support en phase solide produit de la sorte pour effectuer la synthèse d'oligodésoxyribonucléotide à l'aide des processus phosphite-triester ou phosphotriester.
PCT/US1993/006214 1992-07-09 1993-06-29 Support solide organique derive utilise pour effectuer la synthese d'acides nucleiques WO1994001446A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91022392A 1992-07-09 1992-07-09
US07/910,223 1992-07-09

Publications (2)

Publication Number Publication Date
WO1994001446A2 WO1994001446A2 (fr) 1994-01-20
WO1994001446A3 true WO1994001446A3 (fr) 1994-03-03

Family

ID=25428489

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1993/006214 WO1994001446A2 (fr) 1992-07-09 1993-06-29 Support solide organique derive utilise pour effectuer la synthese d'acides nucleiques

Country Status (1)

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WO (1) WO1994001446A2 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ240354A (en) * 1990-10-26 1995-03-28 Genta Inc Polymeric reagent containing nucleoside moiety bound through a linking group suitable for solid phase synthesis of nucleotide oligomers
US5554501A (en) * 1992-10-29 1996-09-10 Beckman Instruments, Inc. Biopolymer synthesis using surface activated biaxially oriented polypropylene
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
AU735440B2 (en) * 1996-02-09 2001-07-05 Cornell Research Foundation Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO1997045559A1 (fr) 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection de differences dans des sequences d'acides nucleiques utilisant une combinaison de la detection par ligase et de reactions d'amplification en chaine par polymerase
AU6412799A (en) 1998-10-05 2000-04-26 Mosaic Technologies Reverse displacement assay for detection of nucleic acid sequences
US6995259B1 (en) 1998-10-23 2006-02-07 Sirna Therapeutics, Inc. Method for the chemical synthesis of oligonucleotides
AU2001293366A1 (en) 2000-04-14 2001-10-30 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
CN1302005C (zh) * 2000-12-05 2007-02-28 艾夫西亚有限公司 硫代磷酸寡核苷酸的制备方法
US6768005B2 (en) 2000-12-20 2004-07-27 Avecia Limited Process
US7205399B1 (en) 2001-07-06 2007-04-17 Sirna Therapeutics, Inc. Methods and reagents for oligonucleotide synthesis
US7279261B2 (en) 2005-01-13 2007-10-09 Xerox Corporation Emulsion aggregation toner compositions

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982003079A1 (fr) * 1981-03-10 1982-09-16 Inc Bioresearch Synthese rapide en phase solide d'oligonucleotides par l'activation d'oxychlorure de phosphore
WO1985001051A1 (fr) * 1983-09-02 1985-03-14 Molecular Biosystems, Inc. Systeme de support polymere d'oligonucleotides
US4659774A (en) * 1985-11-01 1987-04-21 American Hoechst Corporation Support for solid-phase oligonucleotide synthesis
WO1992007882A1 (fr) * 1990-10-26 1992-05-14 Genta Incorporated Reactifs polymeres organiques non aromatiques pour la synthese en phase solide d'oligomeres
WO1992007864A1 (fr) * 1990-10-26 1992-05-14 Genta Incorporated Procede ameliore de synthese d'oligomeres

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1982003079A1 (fr) * 1981-03-10 1982-09-16 Inc Bioresearch Synthese rapide en phase solide d'oligonucleotides par l'activation d'oxychlorure de phosphore
WO1985001051A1 (fr) * 1983-09-02 1985-03-14 Molecular Biosystems, Inc. Systeme de support polymere d'oligonucleotides
US4659774A (en) * 1985-11-01 1987-04-21 American Hoechst Corporation Support for solid-phase oligonucleotide synthesis
WO1992007882A1 (fr) * 1990-10-26 1992-05-14 Genta Incorporated Reactifs polymeres organiques non aromatiques pour la synthese en phase solide d'oligomeres
WO1992007864A1 (fr) * 1990-10-26 1992-05-14 Genta Incorporated Procede ameliore de synthese d'oligomeres

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KATZHENDLER J. ET AL: "THE EFFECT OF SPACER, LINKAGE AND SOLID SUPPORT ON THE SYNTHESIS OF OLIGONUCLEOTIDES", TETRAHEDRON, vol. 45, no. 9, 1989, OXFORD, GB, pages 2777 - 2792, XP026629874, DOI: doi:10.1016/S0040-4020(01)80108-3 *

Also Published As

Publication number Publication date
WO1994001446A2 (fr) 1994-01-20

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