Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B23—MACHINE TOOLS; METAL-WORKING NOT OTHERWISE PROVIDED FOR
  • B23P—METAL-WORKING NOT OTHERWISE PROVIDED FOR; COMBINED OPERATIONS; UNIVERSAL MACHINE TOOLS
  • B23P15/00—Making specific metal objects by operations not covered by a single other subclass or a group in this subclass
  • B23P15/28—Making specific metal objects by operations not covered by a single other subclass or a group in this subclass cutting tools
  • B23P15/40—Making specific metal objects by operations not covered by a single other subclass or a group in this subclass cutting tools shearing tools
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/02—Devices for withdrawing samples
  • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
  • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N1/00—Sampling; Preparing specimens for investigation
  • G01N1/02—Devices for withdrawing samples
  • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
  • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
  • G01N2001/061—Blade details

Definitions

• the present invention relates to a microtome knife made of ⁇ -alumina single crystal, and particularly to a ultra-microtome knife and its manufacturing method. About.
• Ultramicron 7 is used for cutting ultra-thin sections for electron microscopy from materials such as organic tissues. .
• This ultra-thin section has a thickness of ⁇ / mm and has a number of defects exceeding the level of hindering electron microscopy.
• Knife has the following problems.
• the production of Daimon Mondai 7 requires special ripening and know-how, its production technology is not open to the public, and only a few people in the world It is said that it is manufactured by the hands of the Japanese people, and it is very expensive and very difficult to access.
• glass sniffs are cheap, but they have problems with their sharpness and durability, and if they are not manufactured and cut, their appearance will be sure. It is not recognized, and even if it comes out well, the performance will be reduced after several cuts, so that the observer of the slice itself cuts out the slice. The big problem is that you have to make it every time you think.
• microtomizers we have considered that no one exists in the market, and that no one has ever grown up, and that In order to manufacture microtomizers, we have been conducting diligent research, and have finally obtained a small number of ultra-thin sections that do not have any scratches that may hinder electron microscope observation. In particular, we succeeded in manufacturing a micro-microtome knife that could be cut several hundred times from ct-alumina single crystal, and completed the present invention. .
• the present invention is based on the fact that the present invention is a knife composed of an X-alumina single crystal and having two mating ridges at its leading edge.
• the microscopic observation of about 00 times or more there is a problem that obstructs electron microscope observation, which is characterized in that it has substantially no chipped part that can be recognized.
• ultra-mixed seedlings that can cut ultra-thin sections for scratchless electron microscopy several hundred times or more.o
• the present invention provides a method for cutting a parallelepiped of a £ -alumina single crystal, grinding the surface at an appropriate angle with respect to the / face of the parallelepiped, and forming a two-sided intersection thereof.
• the microscope is further subjected to a microscope with a magnification of about s-oo or more. Therefore, it is difficult to observe the electron microscopy, which is a special feature of chemical polishing until the chippings that can be found are not substantially present over the entire ridge line. Electron microscope without scratches
• a single crystal of t-alumina which is obtained by cutting out a piece and causing chipping after use, or having a reduced sharpness, is used.
• the knife whose cutting edge is the intersecting ridge line of the above, until the chipped portion that can be recognized by microscopic observation with a magnification of about 0 or more over the entire ridge line is substantially eliminated. It relates to a method for regenerating ultra-micro mouth tom-nife, which specializes in chemical polishing.
• Fig. / Is an enlarged perspective view of the knife of the present invention.
• the 2nd gI (A) is a micrograph of a knife edge with a ridge notch (photo magnification / J00 times), and (B) is a microtome using a 7 in (A).
• FIG. ( ⁇ ) shows a microscope photograph of a knife edge without edge chipping (photo magnification / JOO times), and (B) shows a microphone mouth with a knife of (A).
• a micrograph of the unscratched section (photograph magnification ⁇ 2) obtained from the micro-tome using the knife in (A) above is shown in a dog. The myocardium was embedded in epoxy resin, and a section was cut.
• Figure ⁇ is a photomicrograph ( ⁇ / 3 °, dark field) of the polished surface of ⁇ -Alumina single crystal after mechanical polishing.
• FIG. 2 is a photomicrograph showing the relationship between a chipped edge of a knife and an etch bit (dislocation defect). Is a low-quality aluminum single crystal with a large chip, and is a high quality Ct-alumina single crystal. Photomicrograph
• the knife according to the present invention is approximately the same
• the first / S is an enlarged perspective ⁇ of the knife of Honoki Akira.
• / is a ridge line
• 2 and J are two faces forming the ridge line /. Is the angle between the surface 2 and the surface.
• 3 is a plane parallel to the ridge line /
• ⁇ is a plane perpendicular to the ridge line /.
• the knife has a face
• the t-alumina single crystal has a C face (000 /) at 4.
• the surface forming the cutting edge ⁇ 2, J should be selected in consideration of sufficient sharpness of section cutting efficiency and the durability of the cutting edge. It can be used satisfactorily within the range of ⁇ 7 ⁇ f. O
• the cutting edge of the wire / is theoretically too sharp, has poor durability, and has too large roundness. Inferior in taste However, it is difficult to accurately measure the radius of the cutting edge and the radius of curvature of the tip, so the range of the necessary and sufficient radius of curvature of the cutting edge is specified. Although it is difficult to do so, it is not difficult to produce a cutting edge with sufficient sharpness and durability for practical use.
• Ultra-thin sections can be judged by using an optical microscope with a magnification of about ⁇ ⁇ or more to determine whether or not the cutting edge does not cause scratches to the extent that it hinders electron microscopy. What we can do is rather surprising. This finding is one of the important grounds of the present invention.
• Fig. 2 is a microscopic photograph of the knife edge taken with a microscope (magnification magnification) ( ⁇ ) and the knife cut through a microtome. Micrograph showing the scratches on the protruded section (photo magnification / 100x) (shows 3 ⁇ 4. (In ⁇ , the blade is shown as a white part. Shown with an arrow.) Thus, it can be seen that the lack of the blade seen in ( ⁇ ) corresponds well with the scratches seen in ( ⁇ ).
• Fig. 2 shows the same magnification as that shown in Fig.
• the production of the ultra-micron life of the present invention was first performed by ⁇
• the formed ridge line / has many chippings, and no effective knife is obtained.
• a mechanically distorted layer is formed on the polished surface by the mechanical polishing and remains. If this surface is further polished with a mechanical or chemical polishing (mechano-chemical polishing), for example, a dispersion of colloidal silica is polished, A chemical reaction occurs between the surface aluminum and the Kodiri-shiri force, which is an abrasive, to form a mulite layer, which is mechanically The removal leaves a much smoother aluminum surface. This surface shows almost no flaws on the surface even with a microscope, and the surface strained layer becomes extremely small. Even with such mechanical and chemical polishing, there can be obtained a super-mixed tom-nife that gives ultra-thin sections without any scratches, but the probability is too small. It ’s a big deal.
• mm plane (40.), aa plane (), ma (i3 °) (m: ⁇ / 0/0 ⁇ , a: ⁇ / 2/0 ⁇ ) is acceptable. This is more desirable.
• One may be the low-index surface of m or a, and the other may be any surface. Also, it is not necessary to set both surfaces to the low index surface.
• a conventional polishing method using, for example, a diamond fine powder may be used. It is advisable to use the customary methods of using felicitc silica, but it is not necessarily limited to these methods ⁇
• the chemical polishing method requires that an appropriate chemical polishing agent capable of dissolving Ct-alumina at an appropriate speed can be used, as long as the function is achieved.
• an appropriate chemical polishing agent capable of dissolving Ct-alumina at an appropriate speed
• polishing is not a problem because surface J is parallel to surface. According to this method, many nail-blocks are attached to each other, and the surface 2 of each book is polished as one plane. It is desirable that the agent layer be as thin as possible. Otherwise, when polishing, the soft adhesive layer is first cut by the fine abrasive particles of the hard abrasive, and the ridge forming the cutting edge is rounded off, resulting in a very thin layer. The reason for this is that the section is rounded to an insufficient degree so that the section can be cut well. In this sense, the thickness of the adhesive layer should not exceed.
• the ultra-microtome knife of the cow invention is used for mechanical polishing, mechanical cutting, or cutting when a number of ultra-thin sections are cut out and then chipped or become dull.
• chemical polishing alone can be used to regenerate the composite knife of ultra-mixtures without chemical polishing.
• the m-side of the side surface was polished as it is, but the m-side of the hexahedral slope was polished, and the m-side of the hexahedron was Plane m plane
• the thickness of the adhesive layer was about // ⁇ , and when the thickness was increased, the diameter of diamond particles was reduced. The edge of the knives may be erased during polishing by grinding, and the sharpness of the knife edge may become poor.Then, the mechanical and chemical polishing by colloidal silica is required.
• a 7 was made in the same manner as in Example 1 except that the two faces forming the ridge forming the tip were designated as a-face and & -face (the intersection angle was). Very similar results were obtained for ultrathin sections.

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• Analytical Chemistry (AREA)
• Immunology (AREA)
• Physics & Mathematics (AREA)
• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• General Health & Medical Sciences (AREA)
• Biochemistry (AREA)
• Mechanical Engineering (AREA)
• General Physics & Mathematics (AREA)
• Engineering & Computer Science (AREA)
• Pathology (AREA)
• Sampling And Sample Adjustment (AREA)
• Crystals, And After-Treatments Of Crystals (AREA)

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Applications Claiming Priority (2)

Publications (1)

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ID=14824571

Family Applications (1)

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Citations (1)

• 1980
  • 1980-09-03 JP JP55121979A patent/JPS5746141A/ja active Granted
• 1981
  • 1981-08-14 EP EP19810902249 patent/EP0059220A4/en not_active Withdrawn
  • 1981-08-14 WO PCT/JP1981/000177 patent/WO1982000890A1/ja not_active Ceased
  • 1981-09-01 IT IT8123709A patent/IT8123709A0/it unknown
  • 1981-09-02 CA CA000385042A patent/CA1163888A/en not_active Expired

Patent Citations (1)

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