US7273700B2 - Nucleic acid probe and novel method of assaying nucleic acid using the same - Google Patents
Nucleic acid probe and novel method of assaying nucleic acid using the same Download PDFInfo
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- US7273700B2 US7273700B2 US10/399,407 US39940703A US7273700B2 US 7273700 B2 US7273700 B2 US 7273700B2 US 39940703 A US39940703 A US 39940703A US 7273700 B2 US7273700 B2 US 7273700B2
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- United States
- Prior art keywords
- nucleic acid
- dye
- labeled
- target nucleic
- probe
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
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- 239000007836 KH2PO4 Substances 0.000 description 1
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- 229910052786 argon Inorganic materials 0.000 description 1
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- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
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- 238000002425 crystallisation Methods 0.000 description 1
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- IERHLVCPSMICTF-UHFFFAOYSA-N cytidine monophosphate Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(COP(O)(O)=O)O1 IERHLVCPSMICTF-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
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- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 238000002509 fluorescent in situ hybridization Methods 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 150000003840 hydrochlorides Chemical class 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
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- 238000007901 in situ hybridization Methods 0.000 description 1
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- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
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- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 125000000714 pyrimidinyl group Chemical group 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- 229940104230 thymidine Drugs 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001-090556 | 2001-03-27 | ||
| JP2001090556 | 2001-03-27 | ||
| PCT/JP2002/003013 WO2002077224A1 (fr) | 2001-03-27 | 2002-03-27 | Nouvelle sonde d'acide nucleique et nouveau procede d'essai d'acides nucleiques faisant appel a ladite sonde |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| US20050048485A1 US20050048485A1 (en) | 2005-03-03 |
| US7273700B2 true US7273700B2 (en) | 2007-09-25 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US10/399,407 Expired - Fee Related US7273700B2 (en) | 2001-03-27 | 2002-03-27 | Nucleic acid probe and novel method of assaying nucleic acid using the same |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US7273700B2 (de) |
| EP (1) | EP1321520A4 (de) |
| CA (1) | CA2424614A1 (de) |
| WO (1) | WO2002077224A1 (de) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140234838A1 (en) * | 2011-10-14 | 2014-08-21 | Becton, Dickinson And Company | Square wave thermal cycling |
| US10428371B2 (en) | 2014-03-31 | 2019-10-01 | Kabushiki Kaisha Dnaform | Fluorescent labeled single-stranded nucleic acid and use thereof |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7273700B2 (en) * | 2001-03-27 | 2007-09-25 | National Institute Of Advanced Industrial Science And Technology | Nucleic acid probe and novel method of assaying nucleic acid using the same |
| BRPI0414987A (pt) | 2003-10-01 | 2006-11-21 | Wallac Oy | ensaio aperfeiçoado homogêneo de transferência de energia com resolução no tempo |
| US20050233332A1 (en) * | 2004-04-14 | 2005-10-20 | Collis Matthew P | Multiple fluorophore detector system |
| GB2433993B (en) * | 2004-10-19 | 2010-04-28 | Wallac Oy | Single molecule TR-FRET probe |
| JP2007054048A (ja) * | 2005-07-27 | 2007-03-08 | Sony Corp | ハイブリダイゼーション検出方法 |
| US8057989B2 (en) | 2006-02-10 | 2011-11-15 | The University Of Hong Kong | Label-free optical sensing and characterization of biomolecules by d8 or d10 metal complexes |
| US8309304B2 (en) | 2006-02-10 | 2012-11-13 | The University Of Hong Kong | Label-free optical sensing and characterization of biomolecules by d8 or d10 metal complexes |
| EP1999272B1 (de) | 2006-03-21 | 2017-11-01 | Koninklijke Philips N.V. | Mikroelektronische sensorvorrichtung mit sensoranordnung |
| CN101405410B (zh) * | 2006-03-21 | 2013-06-19 | 皇家飞利浦电子股份有限公司 | 具有传感器阵列的微电子传感器装置 |
| KR101171635B1 (ko) * | 2006-08-08 | 2012-08-09 | 아크레이 가부시키가이샤 | 변이의 검출 방법 및 그것에 이용하는 키트 |
| DE102007055386B4 (de) * | 2007-11-20 | 2015-07-16 | Boehringer Ingelheim Vetmedica Gmbh | Verfahren zur Kalibrierung eines Sensorelements |
| EP2224017A4 (de) * | 2007-12-26 | 2011-05-04 | Arkray Inc | Verfahren zur erhöhung der nukleinsäure-zielsequenz und dafür verwendete sonde |
| WO2009102844A2 (en) * | 2008-02-13 | 2009-08-20 | The Marine Biological Laboratory | Methods and compositions for identifying cells by combinatorial fluoresence imaging |
| US10706742B2 (en) | 2017-01-17 | 2020-07-07 | Texas Tech University System | Method and system for creating a synthetic pulse |
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- 2002-03-27 US US10/399,407 patent/US7273700B2/en not_active Expired - Fee Related
- 2002-03-27 EP EP02713215A patent/EP1321520A4/de not_active Withdrawn
- 2002-03-27 WO PCT/JP2002/003013 patent/WO2002077224A1/ja active Application Filing
- 2002-03-27 CA CA002424614A patent/CA2424614A1/en not_active Abandoned
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140234838A1 (en) * | 2011-10-14 | 2014-08-21 | Becton, Dickinson And Company | Square wave thermal cycling |
| US9745623B2 (en) * | 2011-10-14 | 2017-08-29 | Becton Dickinson And Company | Square wave thermal cycling |
| US10669575B2 (en) | 2011-10-14 | 2020-06-02 | Becton, Dickinson And Company | Square wave thermal cycling |
| US10428371B2 (en) | 2014-03-31 | 2019-10-01 | Kabushiki Kaisha Dnaform | Fluorescent labeled single-stranded nucleic acid and use thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002077224A1 (fr) | 2002-10-03 |
| US20050048485A1 (en) | 2005-03-03 |
| CA2424614A1 (en) | 2003-04-02 |
| EP1321520A1 (de) | 2003-06-25 |
| EP1321520A4 (de) | 2003-09-03 |
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