BRPI0414987A - ensaio aperfeiçoado homogêneo de transferência de energia com resolução no tempo - Google Patents

ensaio aperfeiçoado homogêneo de transferência de energia com resolução no tempo

Info

Publication number
BRPI0414987A
BRPI0414987A BRPI0414987-4A BRPI0414987A BRPI0414987A BR PI0414987 A BRPI0414987 A BR PI0414987A BR PI0414987 A BRPI0414987 A BR PI0414987A BR PI0414987 A BRPI0414987 A BR PI0414987A
Authority
BR
Brazil
Prior art keywords
energy transfer
transfer test
improved time
resolved energy
microsecond
Prior art date
Application number
BRPI0414987-4A
Other languages
English (en)
Inventor
Ville Laitala
Original Assignee
Wallac Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FI20045022A external-priority patent/FI20045022A0/fi
Application filed by Wallac Oy filed Critical Wallac Oy
Publication of BRPI0414987A publication Critical patent/BRPI0414987A/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)
  • Escalators And Moving Walkways (AREA)

Abstract

"ENSAIO APERFEIçOADO HOMOGêNEO DE TRANSFERêNCIA DE ENERGIA COM RESOLUçãO NO TEMPO". A presente invenção refere-se a um método para aperfeiçoamento da sensibilidade de detecção em ensaios homogêneos de bioafinidade, baseados em TR-FRET. A sensibilidade é aperfeiçoada mediante uso de doadores de longo tempo de vida, junto com uma alta eficiência de transferência de energia e mediante realização da detecção da emissão à base de transferência de energia do aceitador em um intervalo de tempo que é aberto após um atraso de 1 microssegundo ou mais, mas inferior a 50 microssegundos, calculado a partir da excitação do doador e em que o intervalo de tempo apresenta uma amplitude de 1 microssegundo ou mais, porém inferior a 100 micrassegundos. A invenção concerne também o uso do aperfeiçoado método em ensaios de múltiplos analisados. Além disso, a invenção concerne um dispositivo adequado para realização do dito método aperfeiçoado.
BRPI0414987-4A 2003-10-01 2004-09-20 ensaio aperfeiçoado homogêneo de transferência de energia com resolução no tempo BRPI0414987A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US48145603P 2003-10-01 2003-10-01
FI20045022A FI20045022A0 (fi) 2004-01-30 2004-01-30 Parannettu, energiansiirtoon pohjautuva, aika-erotteinen homogeeninen määritysmenetelmä
PCT/FI2004/000550 WO2005033709A1 (en) 2003-10-01 2004-09-20 Improved homogeneous time-resolved energy transfer assay

Publications (1)

Publication Number Publication Date
BRPI0414987A true BRPI0414987A (pt) 2006-11-21

Family

ID=34424841

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0414987-4A BRPI0414987A (pt) 2003-10-01 2004-09-20 ensaio aperfeiçoado homogêneo de transferência de energia com resolução no tempo

Country Status (8)

Country Link
US (1) US7846658B2 (pt)
EP (1) EP1673634B1 (pt)
CN (1) CN1886661B (pt)
AT (1) ATE403870T1 (pt)
BR (1) BRPI0414987A (pt)
CA (1) CA2541075C (pt)
DE (1) DE602004015629D1 (pt)
WO (1) WO2005033709A1 (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0311948D0 (en) * 2003-05-23 2003-06-25 Stockport Innovation Ltd Light emitting probes
US7826052B2 (en) 2005-05-24 2010-11-02 Hidex Oy Correction method and measurement device for anti-stokes photoluminescence measurement
WO2010125314A1 (fr) * 2009-04-30 2010-11-04 Cis-Bio International Procede de detection de composes modulateurs de dimeres de proteines membranaires a domaine vft
WO2011039953A1 (ja) * 2009-09-29 2011-04-07 三井造船株式会社 Ｆｒｅｔ測定方法及び装置
US8834846B2 (en) 2010-05-06 2014-09-16 Bruker Biospin Corporation Fluorescent NIRF activatable probes for disease detection
CN102353790B (zh) * 2011-06-25 2014-03-05 南方医科大学 一种甲胎蛋白的均相时间分辨荧光分析方法
US20130203068A1 (en) * 2012-02-06 2013-08-08 Perkinelmer Biosignal, Inc. Dual-acceptor time-resolved-fret
FR2993668B1 (fr) * 2012-07-17 2015-07-03 Cisbio Bioassays Methode theranostique basee sur la detection de dimeres her2-her2
CN105324493B (zh) * 2013-04-15 2018-02-23 沃拉克有限公司 用于测量强度的串扰校准的方法和设备
CN104714014B (zh) * 2013-12-16 2016-09-21 南昌大学 基于两种量子点之间能量转移的黄曲霉毒素b1检测方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8420521D0 (en) * 1984-08-13 1984-09-19 Hsc Res Dev Corp Fluorescent label
US6576419B1 (en) 1993-07-23 2003-06-10 University Of Utah Research Foundation Assay procedure using fluorogenic tracers
US5622821A (en) 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
FI963989A (fi) * 1996-10-04 1998-04-05 Wallac Oy Homogeenisiä määritysmenetelmiä, jotka perustuvat luminesenssienergiasiirtoon
US5998146A (en) * 1998-07-17 1999-12-07 Wallac Oy Homogeneous luminescence assay method based on energy transfer
AU5492499A (en) * 1998-08-21 2000-03-14 University Of Virginia Patent Foundation Signal generating oligonucleotide-based biosensor
US6218124B1 (en) 1999-08-27 2001-04-17 Pe Corporation Method for detecting oligonucleotides using UV light source
US6613531B2 (en) * 2000-03-30 2003-09-02 Wisconsin Alumni Research Foundation Sigma binding region of RNA polymerase and uses thereof
ATE300050T1 (de) * 2000-11-16 2005-08-15 Hoffmann La Roche Farbstoffpaare für fluoreszenz-resonanz-energie- transfer (fret) messungen
EP1366351B1 (en) * 2001-02-28 2008-12-10 GE Healthcare Niagara Inc. An imaging fluorometer for time resolved fluorescence
DE10111392A1 (de) * 2001-03-09 2002-09-12 Chromeon Gmbh Bioanalytisches Messverfahren unter Verwendung von Oxidasen
US7273700B2 (en) 2001-03-27 2007-09-25 National Institute Of Advanced Industrial Science And Technology Nucleic acid probe and novel method of assaying nucleic acid using the same
DE10155160A1 (de) * 2001-11-12 2003-05-22 Chromeon Gmbh Bioanalytische Messverfahren zur Bestimmung von Katalasen und Peroxidasen, deren Konjugate, Substrate, Aktivatoren und Inhibitoren
ATE370415T1 (de) 2003-03-28 2007-09-15 Tero Soukka Bioassay mit energietransfer mit homogener lumineszenz
ATE458200T1 (de) * 2003-09-12 2010-03-15 Life Technologies Corp Anwendungen des resonanzenergietransfers zwischen terbium und gfp

Also Published As

Publication number Publication date
ATE403870T1 (de) 2008-08-15
US7846658B2 (en) 2010-12-07
DE602004015629D1 (de) 2008-09-18
CA2541075A1 (en) 2005-04-14
CA2541075C (en) 2010-04-27
CN1886661A (zh) 2006-12-27
EP1673634A1 (en) 2006-06-28
WO2005033709A1 (en) 2005-04-14
US20050123957A1 (en) 2005-06-09
CN1886661B (zh) 2010-06-02
EP1673634B1 (en) 2008-08-06

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Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]