US5272354A - Apparatus for imaging particles in a liquid flow - Google Patents
Apparatus for imaging particles in a liquid flow Download PDFInfo
- Publication number
- US5272354A US5272354A US07/933,737 US93373792A US5272354A US 5272354 A US5272354 A US 5272354A US 93373792 A US93373792 A US 93373792A US 5272354 A US5272354 A US 5272354A
- Authority
- US
- United States
- Prior art keywords
- image
- particles
- signal
- particle
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 239000002245 particle Substances 0.000 title claims abstract description 141
- 238000003384 imaging method Methods 0.000 title claims abstract description 57
- 239000007788 liquid Substances 0.000 title claims abstract description 22
- 238000001514 detection method Methods 0.000 claims description 25
- 230000003595 spectral effect Effects 0.000 claims description 4
- 239000000725 suspension Substances 0.000 abstract description 3
- 239000008280 blood Substances 0.000 abstract description 2
- 210000004369 blood Anatomy 0.000 abstract description 2
- 239000010419 fine particle Substances 0.000 abstract description 2
- 229920000642 polymer Polymers 0.000 abstract description 2
- 210000002700 urine Anatomy 0.000 abstract description 2
- 230000005540 biological transmission Effects 0.000 description 9
- 238000010586 diagram Methods 0.000 description 6
- 229910052724 xenon Inorganic materials 0.000 description 6
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 6
- 230000001186 cumulative effect Effects 0.000 description 4
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- 229910052736 halogen Inorganic materials 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- 230000003321 amplification Effects 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
- G01N15/1427—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement with the synchronisation of components, a time gate for operation of components, or suppression of particle coincidences
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J1/00—Photometry, e.g. photographic exposure meter
- G01J1/42—Photometry, e.g. photographic exposure meter using electric radiation detectors
- G01J1/44—Electric circuits
- G01J2001/4446—Type of detector
- G01J2001/4493—Type of detector with image intensifyer tube [IIT]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1477—Multiparameters
Definitions
- the present invention relates to an apparatus for imaging particles in a liquid flow, and in particular to an imaging flow cytometer for imaging and analyzing particle components in a sample liquid containing particle components such as blood and urine, or fine particles such as organic high polymer particles in suspension, or the like, and more particularly to an imaging flow cytometer capable of obtaining a clear still image of particles flowing at high speed (for example, 5 m/sec. or more) even with a light source of a long light emission time such as a lamp, by using an image intensifier with a high speed gate function, in a flow cytometer with still imaging function.
- high speed for example, 5 m/sec. or more
- a flow cytometer As conventionally shown in FIG. 1, it is generally know to capture a particle image free of vibration, by the combination of a pulse laser light source 29 capable of emitting an intense light only for a moment and a video camera 43.
- a sheath flow 15 is formed by passing sheath liquid around the sample flow including the particles 16 to be detected, and this sample fine flow 15 is illuminated with laser light from an argon laser generator 10.
- the light signal (the scattered light or fluorescent light) from the particle is detected by a light detector (photomultiplier or the like) 22, and a signal S1 is sent to a signal processing unit 24 to be processed, thereby analyzing the particles.
- the sheath flow is a flow covered with a laminar sheath liquid around the suspension of particles in order to pass the particles neatly in one row precisely in the middle of the liquid flow.
- a pulse emission trigger signal S4 also shown in FIG. 1 is a pulse emission trigger signal S4, a laser power supply 27, an image processing unit 46, condenser lenses 12, 31, objective lenses 20, 33, a projection lens 41, and a beam stopper 18.
- the Japanese Laid-open Patent Sho. 63-94156 discloses a flow cytometer in which the light source for detecting particles is always illuminated, passing of a cell is detected by a cell detector, and after delaying for a specific time in a delay circuit, the light source for the laser pulse for imaging is turned on to picture the cell.
- the laser light source since the laser light source possesses a high coherence, the interference fringe is often obvious in the obtained particle image, so that the image quality is not so high. Besides, since the laser light source presents a monochromatic light, a color image of the particles is not obtained. Besides, the pulse laser light source, of the gas type, is large in size, and the power source is also large in scale, and is very expensive.
- the luminous time of the xenon flash lamp is generally long, 1 ⁇ sec. or more, and in this case the image may deviate unless the sheath flow velocity is 0.3 m/sec. or less. At the flow velocity of 0.3 m/sec., however, the intrinsic high processing ability of the flow cytometer, that is, the large number of cells analyzed per unit of time is not achieved.
- the present invention provides a first imaging flow cytometer for forming a sheath flow by passing sheath liquid around a sample flow containing particles to be detected, emitting light to this sample fine flow, detecting light signals from the particles, processing the signals in a signal processing unit, and analyzing the particles, comprising:
- an image intensifier to be focused by the transmission light of the particles in the sample fine flow region
- a video camera for capturing the image of the output plane of the image intensifier
- the image intensifier is an image intensifier provided with a high speed gate
- the signal processing unit generates a trigger signal S2 for emitting the light from the light source for imaging a particle by delaying a specific time from detection of the particle signal S1, and while the light source is radiating, a gate signal S3 for opening the shutter of the image intensifier is generated.
- the present invention presents a second imaging flow cytometer, in which, in the first apparatus, the light source for imaging particles is always radiated, and the signal processing unit generates a gate signal S3 for opening the shutter of the image intensifier by a specific time delay from the detection of particle signal S1.
- the present invention also presents a third imaging flow cytometer for forming a sheath flow by passing sheath liquid around a sample flow containing particles to be detected, emitting light to this sample fine flow, detecting light signals from the particles, processing the signal in a signal processing unit, and analyzing the particles, comprising:
- a light source for imaging particles for emitting incoherent light to the sample fine flow region downstream of the detection region of the particle detecting light
- spectral means for dividing the transmission light of the particles in the sample fine flow region into components of three wavelength regions of red, green and blue,
- an image processing unit for processing the video signals from the video cameras as signals R, G, B, wherein
- the image intensifiers are image intensifiers provided with high speed gates, and
- the signal processing unit generates a trigger signal S2 for emitting the light from the light source for imaging a particle by delaying for a specific time from the detection of the particle signal S1, and while the light source is being radiated, a gate signal S3 for opening the shutters of the image intensifiers is generated.
- the present invention also presents a fourth imaging flow cytometer, in which, in the third apparatus, the light source for imaging particles is always radiated, and the signal processing unit generates a gate signal S3 for opening the shutters of the image intensifiers in a specific time delay from the detection of the particle signal S1.
- the apparatus of the present invention comprises two systems, that is, the particle detecting system and the particle imaging system.
- the particle imaging system is installed at the downstream side of the particle detecting system in the sample fine flow.
- the signal processing unit When the particle detection signal S1 is detected, the signal processing unit generates a trigger signal S2 in a specific time delay, and the light source for imaging a particle is illuminated to capture a still image of the particle passing through the sample fine flow by a video camera.
- the delay of a specific time is the time required for the particle to move from the particle detection region to the imaging region.
- the luminous time In order to obtain a still image of the particle flowing at high speed, the luminous time must be short. If the luminous time is long, a blurry image is captured. Besides, a sufficient quantity of light is also needed.
- the image intensifier by generating a gate signal S3 while the particle imaging light source is emitting light, the image intensifier is operated to obtain a still image of the particle.
- the image intensifier intensifies the input feeble image, and produces a bright image.
- the image intensifier with a high speed gate function operates as an image intensifier only when the gate signal is ON. Accordingly, regardless of the luminous time of the light source, by turning on the gate signal for a short time while the light source is luminous, a bright and sharp still image is obtained.
- the still picture obtained by the image intensifier is captured by the video camera, and processed in the image processing unit.
- the transmission light is divided into red, green and blue components, and for these three images there are three image intensifiers with a high speed gate and three monochromatic video cameras, so that video signals of R, G, B components are obtained for one image. That is, one color image is obtained.
- FIG. 1 is a schematic explanatory diagram of a conventional apparatus.
- FIG. 2 is a schematic explanatory diagram showing an embodiment of an imaging flow cytometer of the present invention.
- FIG. 3 is a schematic explanatory diagram showing another embodiment of an imaging flow cytometer of the present invention.
- FIG. 4 is a diagram for explaining the timing of each signal in an imaging flow cytometer of the present invention.
- FIG. 5 is a diagram for explaining the structure and operating principle of an image intensifier.
- FIG. 2 shows an imaging flow cytometer for forming a sheath flow by passing sheath liquid around a sample flow containing particles 16 to be detected, emitting light to this sample fine flow 15, detecting light signals from the particles, processing the signals in a signal processing unit 24, and analyzing the particles, comprising:
- an image intensifier 38 to be focused by the transmission light of the particles in the sample fine flow region
- a video camera 42 for capturing the image of the output plane of the image intensifier 38
- an image processing unit 44 for processing the video signal from the video camera 42, wherein
- the image intensifier 38 is an image intensifier provided with a high speed gate
- the signal processing unit 24 generates a trigger signal S2 for emitting the light from the light source 28 for imaging a particle by delaying a specific time from detection of the particle signal S1, and while the light source 28 is illuminating, a gate signal S3 for opening the shutter of the image intensifier 38 is generated.
- the light source 28 for imaging particles is always radiated, and the signal processing unit 24 generates a gate signal S3 for opening the shutter of the image intensifier 38 by a specific time delay from the detection of the particle signal S1.
- FIG. 3 shows an imaging flow cytometer for forming a sheath flow by passing sheath liquid around a sample flow containing particles 16 to be detected, emitting light to a sample fine flow 15, detecting light signals from the particles, processing the signal in a signal processing unit 24, and analyzing the particles, comprising:
- spectral means 35 for dividing the transmission light of the particles in the sample fine flow region into components of three wavelength regions of red, green and blue,
- image intensifiers 38a, 38b, 38c to be focused by the transmitted light divided in three components
- video cameras 42a, 42b, 42c for capture the images of the output planes of the image intensifiers 38a, 38b, 38c, and
- an image processing unit 45 for processing the video signals from the video cameras 42a, 42b, 42c as signals R, G, B, wherein
- the image intensifiers 38a, 38b, 38c are image intensifiers provided with high speed gates, and
- the signal processing unit 24 generates a trigger signal S2 for emitting the light from the light source 28 for imaging a particle by delaying for a specific time from the detection of the particle signal S1, and while the light source 28 is being radiated, a gate signal S3 for opening the shutters of the image intensifiers 38a, 38b, 38c is generated.
- the light source 28 for imaging particles is always radiated, and the signal processing unit 24 generates a gate signal S3 for opening the shutters of the image intensifiers 38a, 38b, 38c in a specific time delay from the detection of the particle signal S1.
- the apparatus of the present invention comprises two systems, that is, the particle detecting system and the particle imaging system.
- the particle imaging system is installed at the downstream side of the particle detecting system in the sample fine flow 15.
- the signal processing unit 24 When the particle detection signal S1 is detected, the signal processing unit 24 generates a trigger signal S2 in a specific time delay, and the light source 28 for imaging a particle is illuminated to capture a still image of the particle passing through the sample fine flow by a video camera 42.
- the specific time delay is the time required for the particle to move from the particle detection region to the image capturing region.
- the luminous time In order to obtain a still image of the particle flowing at high speed, the luminous time must be short. If the luminous time is long, a blurry image is captured. Besides, a sufficient quantity of light is also needed.
- the image intensifier 38 is operated to obtain a still image of the particle.
- the image intensifier intensifies the input feeble image, and produces a bright image.
- the image intensifier with a high speed gate function operates as an image intensifier only when the gate signal is ON. Accordingly, regardless of the luminous time of the light source, by turning on the gate signal for a short time while the light source is luminous, a bright and sharp still image is obtained.
- the still image obtained by the image intensifier is captured by the video camera 42, and processed in the image processing unit 44.
- the transmission light is divided into red, green and blue components, and for these three images there are three image intensifiers 38a, 38b, 38c each with a high speed gate and three monochromatic video cameras 42a, 42b, 42c, so that video signals of R, G, B components are obtained for one image. That is, one color image is obtained.
- a still image is obtained by always emitting the imaging light source 28, by operating the image intensifier by the gate signal S3 only when desired.
- the apparatus of FIG. 2 is realized by combining the conventional imaging flow cytometer shown in FIG. 1 with the light source 28 for imaging a particle for emitting incoherent light, and the image intensifier 38 with high speed gate to be focused by the transmission light of particles, and others.
- a xenon lamp or a halogen lamp may be used, and the lamp may be either of the flash emission type or of the continuous emission type.
- the sample flow containing the particle 16 to be detected is led into a flow cell 14 composed of a transparent material such as glass and plastic, and a sheath liquid is supplied to cover the circumference of the sample flow, thereby forming a sheath flow
- the laser light from the laser light source 10 is emitted to the sample fine flow 15 through the condenser lens 12.
- the light signal (scattered light or fluorescent light) from the particle is detected by the light detector 22 through objective lens 20 and beam stopper 18, and the signal S1 is sent to the signal processing unit 24 to be processed.
- the strobe luminous trigger signal S2 from the signal processing unit 24 is sent to the power supply of strobe 26 to emit the light from the light source 28 for imaging a particle, and incoherent light is emitted to the sample fine flow region downstream of the detection region of the particle detection light through collimator lens 30 and condenser lens 32.
- the light transmission from the particle passes through the objective lens 34 and the projection lens 36, and is focused on the photoelectric plane (input plane) of the image intensifier 38 with high speed gate function
- the image of the output plane of the image intensifier 38 is projected to the video camera 42 through relay lens 40 to be captured, and the video signal is sent to the image processing unit 44 to be processed.
- FIG. 5 is a diagram for explaining the operating principle of the image intensifier 38.
- the gate function of the image intensifier 38 is realized generally by polarity control of the potential of the photoelectric plane 50 against the microchannel plate (MCP) 56. That is, when the potential of the photoelectric plane 50 is positive, the photoelectrons released from the photoelectric plane 50 not reach the MCP 56, and it thereby serves as a shutter in a closed state. On the contrary, when the potential of the photoelectric plane 50 is negative, the photoelectrons reaches the MCP 56, and the shutter is opened. This response of the gate function is usually as fast as several nanoseconds.
- Numerals 52, 54 are electronic lenses, and 58 is a fluorescent plane.
- the exposure time to the CCD plane of the video camera is 60 nsec. or less. Hence, by setting the gate ON time within 60 nsec., a deflection-free particle image is obtained.
- the control of the timing for turning on the gate of the image intensifier 38 is explained by reference to FIG. 4. From the scattered light or signal S1 of fluorescent intensity obtained from the detection system as the conventional flow cytometer, the particle is detailed passing through the detection area of the flow cell 14. Next, waiting until the particle reaches the video camera capturing area located in the downstream direction of the detection area, the gate of the image intensifier 38 is turned on for a period of scores of nanoseconds. When using a lamp of the flash emission type, first a trigger for illuminating the flash is applied, then the gate is turned on. The time from the issue of the trigger for lighting the flash till the gate of the image intensifier 38 is turned on depends on the delay time t1 from the issue of the trigger to the lamp till the emission intensity L reaches the peak. Meanwhile, S2 is a strobe luminous trigger signal, S3 is a gate ON signal of image intensifier, and tm is the time that the particle moves from the laser detection area to the video camera capturing area.
- photoelectrons depending on the particles image focused on the photoelectric plane 50 are released, and these photoelectrons are put into the MCP (microchannel plate) 56, and are amplified several thousand times.
- the amplified photoelectrons further excite the fluorescent plane 58 which is the output plane, and a particle image amplified several thousand times is obtained.
- the image on the fluorescent plane 58 is focused on the CCD plane of the video camera 42 through the relay lens 40 or optical fiber.
- the video camera 42 is available in the field cumulative type and frame cumulative type depending on whether the light cumulative time is 1/60 sec. or 1/30 sec., and where the vertical resolution is more important, the frame cumulative type should be used.
- the exposure time by the gate function is limited to only one exposure in an even-number field period. One exposure means multiple exposures are prohibited. Therefore, all particles passing through the flow cell detection unit cannot be taken, and an application for taking a specific particle only is desired.
- the light source 28 for particle imaging by using a white light source such as xenon lamp or halogen lamp, a color image of the particle difficult to obtain in the conventional laser light source can be obtained.
- An embodiment for this purpose is shown, in FIG. 3.
- Three sets each of image intensifiers each with a gate function and monochromatic video cameras are provided, and filters or prisms for separating (resolving) the light from the particle into the three primary colors of red, green and blue are disposed before the input planes of the individual image intensifiers 38a, 38b, 38c, and the image is amplified in each color and is captured by the video cameras 42a, 42b, 42c.
- the video signals from the individual video cameras are obtained as color video signals of R (red), G (green) and B (blue).
- Numerals 35a, 35b, 35c are spectral means (for example, dichroic mirrors), and 36a, 36b, 36c are projection lenses, 40a, 40b, 40c are relay lenses, and 45 is an image processing unit.
- the other components and their function are same as in FIG. 2.
- the present invention being thus constructed, brings about the following effects.
- a blur-less, clear particle image of a particle flowing at high speed is obtained by a lamp light source of a relatively long luminous time such as xenon lamp, without using a pulse laser light source of short luminous time and a large quantity of light.
- an incoherent light source such as a xenon lamp
- a particle image without interference fringe may be obtained.
- the lamp is an inexpensive and small light source, and the apparatus may be reduced in cost and in size.
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Signal Processing (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/565,580 USRE35868E (en) | 1991-11-20 | 1995-11-30 | Apparatus for imaging particles in a liquid flow |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP03331282A JP3102935B2 (ja) | 1991-11-20 | 1991-11-20 | イメージングフローサイトメータ |
JP3-331282 | 1991-11-20 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/565,580 Reissue USRE35868E (en) | 1991-11-20 | 1995-11-30 | Apparatus for imaging particles in a liquid flow |
Publications (1)
Publication Number | Publication Date |
---|---|
US5272354A true US5272354A (en) | 1993-12-21 |
Family
ID=18241947
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US07/933,737 Ceased US5272354A (en) | 1991-11-20 | 1992-08-24 | Apparatus for imaging particles in a liquid flow |
US08/565,580 Expired - Lifetime USRE35868E (en) | 1991-11-20 | 1995-11-30 | Apparatus for imaging particles in a liquid flow |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/565,580 Expired - Lifetime USRE35868E (en) | 1991-11-20 | 1995-11-30 | Apparatus for imaging particles in a liquid flow |
Country Status (3)
Country | Link |
---|---|
US (2) | US5272354A (ja) |
EP (1) | EP0543514A3 (ja) |
JP (1) | JP3102935B2 (ja) |
Cited By (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5422712A (en) * | 1992-04-01 | 1995-06-06 | Toa Medical Electronics Co., Ltd. | Apparatus for measuring fluorescent spectra of particles in a flow |
US5436717A (en) * | 1992-04-01 | 1995-07-25 | Toa Medical Electronics Co., Ltd. | Apparatus for analyzing particles |
US5444527A (en) * | 1992-06-12 | 1995-08-22 | Toa Medical Electronics Co., Ltd. | Imaging flow cytometer for imaging and analyzing particle components in a liquid sample |
US5596401A (en) * | 1993-09-16 | 1997-01-21 | Toa Medical Electronics Co., Ltd. | Particle analyzing apparatus using a coherence lowering device |
US5682235A (en) * | 1994-09-23 | 1997-10-28 | Horiba, Ltd. | Dry particle-size distribution measuring apparatus |
US5815264A (en) * | 1994-09-21 | 1998-09-29 | Laser Sensor Technology, Inc | System for acquiring an image of a multi-phase fluid by measuring backscattered light |
US5880835A (en) * | 1992-02-18 | 1999-03-09 | Hitachi, Ltd. | Apparatus for investigating particles in a fluid, and a method of operation thereof |
US6211955B1 (en) | 2000-01-24 | 2001-04-03 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US20020094116A1 (en) * | 2000-08-25 | 2002-07-18 | Amnis Corporation | Method and apparatus for reading reporter labeled beads |
US20020146734A1 (en) * | 2001-02-21 | 2002-10-10 | Amnis Corporation | Method and apparatus for labeling and analyzing cellular components |
US6473176B2 (en) | 1999-01-25 | 2002-10-29 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US6507391B2 (en) | 2000-08-25 | 2003-01-14 | Amnis Corporation | Measuring the velocity of small moving objects such as cells |
US6563583B2 (en) | 2000-10-12 | 2003-05-13 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
US6580504B1 (en) | 1999-01-25 | 2003-06-17 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
US6583865B2 (en) | 2000-08-25 | 2003-06-24 | Amnis Corporation | Alternative detector configuration and mode of operation of a time delay integration particle analyzer |
US20030137661A1 (en) * | 2000-01-24 | 2003-07-24 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
US6608682B2 (en) | 1999-01-25 | 2003-08-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US6608680B2 (en) | 2000-08-25 | 2003-08-19 | Amnis Corporation | TDI imaging system for kinetic studies |
US6671044B2 (en) | 1999-01-25 | 2003-12-30 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells in broad flat flow |
US20040008345A1 (en) * | 2002-05-20 | 2004-01-15 | Nurmikko Arto V. | Optical tracking and detection of particles by solid state energy sources |
US20040021868A1 (en) * | 1999-01-25 | 2004-02-05 | Ortyn William E. | Imaging and analyzing parameters of small moving objects such as cells |
US20040161165A1 (en) * | 2001-04-25 | 2004-08-19 | Amnis Corporation | Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging |
US20040218184A1 (en) * | 1999-01-25 | 2004-11-04 | Amnis Corporation | Imaging platform for nanoparticle detection applied to SPR biomolecular interaction analysis |
US20040217256A1 (en) * | 2000-08-25 | 2004-11-04 | Amnis Corporation | Auto focus for a flow imaging system |
US20040223135A1 (en) * | 2000-08-25 | 2004-11-11 | Amnis Corporation | Methods of calibrating an imaging system using calibration beads |
US20060068371A1 (en) * | 1999-01-25 | 2006-03-30 | Amnis Corporation | Methods for analyzing inter-cellular phenomena |
US20060257884A1 (en) * | 2004-05-20 | 2006-11-16 | Amnis Corporation | Methods for preparing and analyzing cells having chromosomal abnormalities |
US7190832B2 (en) | 2001-07-17 | 2007-03-13 | Amnis Corporation | Computational methods for the segmentation of images of objects from background in a flow imaging instrument |
US20090109432A1 (en) * | 2007-10-26 | 2009-04-30 | Olson Robert J | Systems and methods for submersible imaging flow apparatus |
US7889263B2 (en) | 2000-10-12 | 2011-02-15 | Amnis Corporation | System and method for high numeric aperture imaging systems |
US20110063617A1 (en) * | 2008-04-04 | 2011-03-17 | Hiromi Takahashi | Method and Device for Measuring Circular Dichroism Spectra |
US8005314B2 (en) | 2005-12-09 | 2011-08-23 | Amnis Corporation | Extended depth of field imaging for high speed object analysis |
US20110303859A1 (en) * | 2010-03-10 | 2011-12-15 | Lofstrom Christopher D | Generating Pulse Parameters in a Particle Analyzer |
US8103080B2 (en) | 2004-03-16 | 2012-01-24 | Amnis Corporation | Method for imaging and differential analysis of cells |
US8131053B2 (en) | 1999-01-25 | 2012-03-06 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
US8150136B2 (en) | 2004-03-16 | 2012-04-03 | Amnis Corporation | Image based quantitation of molecular translocation |
US8406498B2 (en) | 1999-01-25 | 2013-03-26 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
US8451524B2 (en) | 2009-09-29 | 2013-05-28 | Amnis Corporation | Modifying the output of a laser to achieve a flat top in the laser's Gaussian beam intensity profile |
US20130242302A1 (en) * | 2012-03-19 | 2013-09-19 | Sony Corporation | Fine particle measurement device |
US8817115B1 (en) | 2010-05-05 | 2014-08-26 | Amnis Corporation | Spatial alignment of image data from a multichannel detector using a reference image |
US20140291551A1 (en) * | 2011-11-19 | 2014-10-02 | Axure Technologies S.A. | Methods and systems for detecting and quantifying petroleum oil based on fluorescence |
US8885913B2 (en) | 1999-01-25 | 2014-11-11 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
US8953866B2 (en) | 2004-03-16 | 2015-02-10 | Amnis Corporation | Method for imaging and differential analysis of cells |
US20150177118A1 (en) * | 2013-12-23 | 2015-06-25 | Palo Alto Research Center Incorporated | Fluidic optical cartridge |
US20160299060A1 (en) * | 2015-04-10 | 2016-10-13 | Blaze Metrics, LLC | System and method for simultaneously performing multiple optical analyses of liquids and particles in a fluid |
US10386290B2 (en) | 2017-03-31 | 2019-08-20 | Life Technologies Corporation | Apparatuses, systems and methods for imaging flow cytometry |
WO2022132667A1 (en) * | 2020-12-14 | 2022-06-23 | Life Technologies Corporation | Systems and methods for improved imaging and fluorescence in flow cytometry and other applications |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69429145T2 (de) * | 1993-08-19 | 2002-07-18 | Hitachi Ltd | Klassifikation und Prüfvorrichtung für Teilchen in einer Flüssigkeit |
JP2826449B2 (ja) * | 1993-09-17 | 1998-11-18 | 株式会社日立製作所 | フロー式粒子画像解析方法およびフロー式粒子画像解析装置 |
JP3290786B2 (ja) * | 1993-11-26 | 2002-06-10 | シスメックス株式会社 | 粒子分析装置 |
JP3165309B2 (ja) | 1993-12-22 | 2001-05-14 | 株式会社日立製作所 | 粒子画像解析装置 |
CN1036093C (zh) * | 1994-01-14 | 1997-10-08 | 中国科学院光电技术研究所 | 粒子测量方法及其粒子探测传感器 |
US6710879B1 (en) | 1997-05-05 | 2004-03-23 | Chemometec A/S | Method and a system for determination of particles in a liquid sample |
NO981574L (no) * | 1998-04-07 | 1999-10-08 | Norsk Hydro As | Metode og utstyr for deteksjon og bestemmelse av mengden etc. av partikler i en vaeske |
US6309886B1 (en) * | 1999-06-04 | 2001-10-30 | The Regents Of The University Of California | High throughput analysis of samples in flowing liquid |
US6813017B1 (en) * | 1999-10-20 | 2004-11-02 | Becton, Dickinson And Company | Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer |
JP4018063B2 (ja) * | 2000-10-12 | 2007-12-05 | アムニス コーポレイション | 画像化システム及びその方法 |
JP3996056B2 (ja) * | 2000-10-12 | 2007-10-24 | アムニス コーポレイション | レポーターラベルビードを読み取るための方法および装置 |
JP4509154B2 (ja) | 2007-09-04 | 2010-07-21 | ソニー株式会社 | 光照射装置、微粒子解析装置及び光照射方法 |
CA2756045A1 (en) * | 2009-03-20 | 2010-09-23 | Biorad Laboratories, Inc. | Serial-line-scan-encoded multi-color fluorescence microscopy and imaging flow cytometry |
JP5321260B2 (ja) * | 2009-06-11 | 2013-10-23 | ソニー株式会社 | 光学的測定装置、並びにフローサイトメーター及び光学的測定方法 |
JP2013015357A (ja) * | 2011-07-01 | 2013-01-24 | Shimadzu Corp | フローサイトメータ |
US8582100B1 (en) * | 2012-06-19 | 2013-11-12 | The United States Of America, As Represented By The Secretary Of The Navy | Remote multisensor optical particle monitor for flowing fluid systems |
CN107850530B (zh) | 2015-05-04 | 2020-11-06 | 港大科桥有限公司 | 用于对样本进行光学成像的装置和方法 |
JP7388004B2 (ja) * | 2019-05-30 | 2023-11-29 | 株式会社レゾナック | 半導体装置の製造方法 |
JP7447897B2 (ja) * | 2019-05-30 | 2024-03-12 | ソニーグループ株式会社 | 光学測定装置及び光学測定システム |
JPWO2020262092A1 (ja) * | 2019-06-26 | 2020-12-30 | ||
JP6745559B1 (ja) * | 2020-03-24 | 2020-08-26 | 株式会社Cybo | イメージングフローサイトメーター、ソート方法、及びキャリブレーション方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4890925A (en) * | 1986-08-11 | 1990-01-02 | Hitachi, Ltd. | Method and apparatus for detecting particular particulate substance |
US4917496A (en) * | 1988-07-11 | 1990-04-17 | Pacific Scientific Company | Particle size measuring instrument with direct scattered light detection |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58105003A (ja) * | 1981-12-18 | 1983-06-22 | Hitachi Denshi Syst Service Kk | カラ−テレビカメラを用いた微小寸法計測装置 |
US4998284A (en) * | 1987-11-17 | 1991-03-05 | Cell Analysis Systems, Inc. | Dual color camera microscope and methodology for cell staining and analysis |
JPH073419B2 (ja) * | 1986-10-07 | 1995-01-18 | 東亜医用電子株式会社 | 流体中の細胞分析方法および装置 |
NO894680L (no) * | 1989-11-24 | 1991-05-27 | Flowtech A S V Harald Steen | Pulsmodulasjon av eksitasjonslyskilden i vaeskestroemcytofotometere. |
JP3049254B2 (ja) * | 1990-02-08 | 2000-06-05 | シスメックス株式会社 | 2種類の光源を備えた光学式粒子分析装置 |
JP3084295B2 (ja) * | 1991-02-27 | 2000-09-04 | シスメックス株式会社 | フローイメージサイトメータ |
-
1991
- 1991-11-20 JP JP03331282A patent/JP3102935B2/ja not_active Expired - Fee Related
-
1992
- 1992-08-24 US US07/933,737 patent/US5272354A/en not_active Ceased
- 1992-10-27 EP EP19920309824 patent/EP0543514A3/en not_active Withdrawn
-
1995
- 1995-11-30 US US08/565,580 patent/USRE35868E/en not_active Expired - Lifetime
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4890925A (en) * | 1986-08-11 | 1990-01-02 | Hitachi, Ltd. | Method and apparatus for detecting particular particulate substance |
US4917496A (en) * | 1988-07-11 | 1990-04-17 | Pacific Scientific Company | Particle size measuring instrument with direct scattered light detection |
Cited By (101)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5880835A (en) * | 1992-02-18 | 1999-03-09 | Hitachi, Ltd. | Apparatus for investigating particles in a fluid, and a method of operation thereof |
US5422712A (en) * | 1992-04-01 | 1995-06-06 | Toa Medical Electronics Co., Ltd. | Apparatus for measuring fluorescent spectra of particles in a flow |
US5436717A (en) * | 1992-04-01 | 1995-07-25 | Toa Medical Electronics Co., Ltd. | Apparatus for analyzing particles |
US5444527A (en) * | 1992-06-12 | 1995-08-22 | Toa Medical Electronics Co., Ltd. | Imaging flow cytometer for imaging and analyzing particle components in a liquid sample |
US5596401A (en) * | 1993-09-16 | 1997-01-21 | Toa Medical Electronics Co., Ltd. | Particle analyzing apparatus using a coherence lowering device |
US5815264A (en) * | 1994-09-21 | 1998-09-29 | Laser Sensor Technology, Inc | System for acquiring an image of a multi-phase fluid by measuring backscattered light |
US5682235A (en) * | 1994-09-23 | 1997-10-28 | Horiba, Ltd. | Dry particle-size distribution measuring apparatus |
US6608682B2 (en) | 1999-01-25 | 2003-08-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US20040021868A1 (en) * | 1999-01-25 | 2004-02-05 | Ortyn William E. | Imaging and analyzing parameters of small moving objects such as cells |
US7925069B2 (en) | 1999-01-25 | 2011-04-12 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
US7634125B2 (en) | 1999-01-25 | 2009-12-15 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
US6473176B2 (en) | 1999-01-25 | 2002-10-29 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US7634126B2 (en) | 1999-01-25 | 2009-12-15 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
US20090190822A1 (en) * | 1999-01-25 | 2009-07-30 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
US8406498B2 (en) | 1999-01-25 | 2013-03-26 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
US6580504B1 (en) | 1999-01-25 | 2003-06-17 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
US7522758B2 (en) | 1999-01-25 | 2009-04-21 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
US8009189B2 (en) | 1999-01-25 | 2011-08-30 | Amnis Corporation | Extended depth of field imaging for high speed object analysis |
US20060066837A1 (en) * | 1999-01-25 | 2006-03-30 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US20090003681A1 (en) * | 1999-01-25 | 2009-01-01 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
US6671044B2 (en) | 1999-01-25 | 2003-12-30 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells in broad flat flow |
US8885913B2 (en) | 1999-01-25 | 2014-11-11 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
US6249341B1 (en) | 1999-01-25 | 2001-06-19 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US7450229B2 (en) | 1999-01-25 | 2008-11-11 | Amnis Corporation | Methods for analyzing inter-cellular phenomena |
US20040080748A1 (en) * | 1999-01-25 | 2004-04-29 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
US7315357B2 (en) | 1999-01-25 | 2008-01-01 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US7221457B2 (en) | 1999-01-25 | 2007-05-22 | Amnis Corporation | Imaging platform for nanoparticle detection applied to SPR biomolecular interaction analysis |
US20040218184A1 (en) * | 1999-01-25 | 2004-11-04 | Amnis Corporation | Imaging platform for nanoparticle detection applied to SPR biomolecular interaction analysis |
US8131053B2 (en) | 1999-01-25 | 2012-03-06 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
US8548219B2 (en) | 1999-01-25 | 2013-10-01 | Amnis Corporation | Detection of circulating tumor cells using imaging flow cytometry |
US20060204071A1 (en) * | 1999-01-25 | 2006-09-14 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
US20060192955A1 (en) * | 1999-01-25 | 2006-08-31 | Amnis Corporation | Imaging platform for nanoparticle detection applied to spr biomolecular interaction analysis |
US8660332B2 (en) | 1999-01-25 | 2014-02-25 | Amnis Corporation | Blood and cell analysis using an imaging flow cytometer |
US7057732B2 (en) | 1999-01-25 | 2006-06-06 | Amnis Corporation | Imaging platform for nanoparticle detection applied to SPR biomolecular interaction analysis |
US20060068371A1 (en) * | 1999-01-25 | 2006-03-30 | Amnis Corporation | Methods for analyzing inter-cellular phenomena |
US6947136B2 (en) | 1999-01-25 | 2005-09-20 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
US6975400B2 (en) | 1999-01-25 | 2005-12-13 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US20030137661A1 (en) * | 2000-01-24 | 2003-07-24 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
US6707551B2 (en) | 2000-01-24 | 2004-03-16 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
US6211955B1 (en) | 2000-01-24 | 2001-04-03 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
US6532061B2 (en) | 2000-08-25 | 2003-03-11 | Amnis Corporation | Measuring the velocity of small moving objects such as cells |
US20060029267A1 (en) * | 2000-08-25 | 2006-02-09 | Amnis Corporation | Method and apparatus for reading reporter labeled beads |
US20040085527A1 (en) * | 2000-08-25 | 2004-05-06 | Basiji David A. | Alternative detector configuration and mode of operation of a time delay integration particle analyzer |
US20020094116A1 (en) * | 2000-08-25 | 2002-07-18 | Amnis Corporation | Method and apparatus for reading reporter labeled beads |
US6507391B2 (en) | 2000-08-25 | 2003-01-14 | Amnis Corporation | Measuring the velocity of small moving objects such as cells |
US7087877B2 (en) | 2000-08-25 | 2006-08-08 | Amnis Corporation | Auto focus for a flow imaging system |
US6906792B2 (en) | 2000-08-25 | 2005-06-14 | Amnis Corporation | Methods of calibrating an imaging system using calibration beads |
US6947128B2 (en) | 2000-08-25 | 2005-09-20 | Amnis Corporation | Alternative detector configuration and mode of operation of a time delay integration particle analyzer |
US6875973B2 (en) | 2000-08-25 | 2005-04-05 | Amnis Corporation | Auto focus for a flow imaging system |
US20040223135A1 (en) * | 2000-08-25 | 2004-11-11 | Amnis Corporation | Methods of calibrating an imaging system using calibration beads |
US20040217256A1 (en) * | 2000-08-25 | 2004-11-04 | Amnis Corporation | Auto focus for a flow imaging system |
US7567695B2 (en) | 2000-08-25 | 2009-07-28 | Amnis Corporation | Method and apparatus for reading reporter labeled beads |
US6583865B2 (en) | 2000-08-25 | 2003-06-24 | Amnis Corporation | Alternative detector configuration and mode of operation of a time delay integration particle analyzer |
US6934408B2 (en) | 2000-08-25 | 2005-08-23 | Amnis Corporation | Method and apparatus for reading reporter labeled beads |
US20050127271A1 (en) * | 2000-08-25 | 2005-06-16 | Amnis Corporation | Auto focus for a flow imagin system |
US6608680B2 (en) | 2000-08-25 | 2003-08-19 | Amnis Corporation | TDI imaging system for kinetic studies |
US6563583B2 (en) | 2000-10-12 | 2003-05-13 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
US7889263B2 (en) | 2000-10-12 | 2011-02-15 | Amnis Corporation | System and method for high numeric aperture imaging systems |
US8379136B2 (en) | 2000-10-12 | 2013-02-19 | Amnis Corporation | System and method for high numeric aperture imaging systems |
US20020146734A1 (en) * | 2001-02-21 | 2002-10-10 | Amnis Corporation | Method and apparatus for labeling and analyzing cellular components |
US7006710B2 (en) | 2001-04-25 | 2006-02-28 | Amnis Corporation | Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging |
US20040161165A1 (en) * | 2001-04-25 | 2004-08-19 | Amnis Corporation | Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging |
US20060198558A1 (en) * | 2001-04-25 | 2006-09-07 | Amnis Corporation | Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging |
US7079708B2 (en) | 2001-04-25 | 2006-07-18 | Amnis Corporation | Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging |
US20060002634A1 (en) * | 2001-04-25 | 2006-01-05 | Amnis Corporation | Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging |
US7286719B2 (en) | 2001-04-25 | 2007-10-23 | Amnis Corporation | Method and apparatus for correcting crosstalk and spatial resolution for multichannel imaging |
US7190832B2 (en) | 2001-07-17 | 2007-03-13 | Amnis Corporation | Computational methods for the segmentation of images of objects from background in a flow imaging instrument |
US20040008345A1 (en) * | 2002-05-20 | 2004-01-15 | Nurmikko Arto V. | Optical tracking and detection of particles by solid state energy sources |
US7064827B2 (en) * | 2002-05-20 | 2006-06-20 | Brown University Research Foundation | Optical tracking and detection of particles by solid state energy sources |
US8571294B2 (en) | 2004-03-16 | 2013-10-29 | Amnis Corporation | Method for imaging and differential analysis of cells |
US8103080B2 (en) | 2004-03-16 | 2012-01-24 | Amnis Corporation | Method for imaging and differential analysis of cells |
US8150136B2 (en) | 2004-03-16 | 2012-04-03 | Amnis Corporation | Image based quantitation of molecular translocation |
US9528989B2 (en) | 2004-03-16 | 2016-12-27 | Amnis Corporation | Image-based quantitation of molecular translocation |
US8953866B2 (en) | 2004-03-16 | 2015-02-10 | Amnis Corporation | Method for imaging and differential analysis of cells |
US8824770B2 (en) | 2004-03-16 | 2014-09-02 | Amnis Corporation | Method for imaging and differential analysis of cells |
US8571295B2 (en) | 2004-03-16 | 2013-10-29 | Amnis Corporation | Method for imaging and differential analysis of cells |
US20060257884A1 (en) * | 2004-05-20 | 2006-11-16 | Amnis Corporation | Methods for preparing and analyzing cells having chromosomal abnormalities |
US8005314B2 (en) | 2005-12-09 | 2011-08-23 | Amnis Corporation | Extended depth of field imaging for high speed object analysis |
US20090109432A1 (en) * | 2007-10-26 | 2009-04-30 | Olson Robert J | Systems and methods for submersible imaging flow apparatus |
US8542357B2 (en) | 2008-04-04 | 2013-09-24 | The University Of Tokyo | Method and device for measuring circular dichroism spectra |
US20110063617A1 (en) * | 2008-04-04 | 2011-03-17 | Hiromi Takahashi | Method and Device for Measuring Circular Dichroism Spectra |
US8451524B2 (en) | 2009-09-29 | 2013-05-28 | Amnis Corporation | Modifying the output of a laser to achieve a flat top in the laser's Gaussian beam intensity profile |
US8754390B2 (en) * | 2010-03-10 | 2014-06-17 | Beckman Coulter, Inc. | Generating pulse parameters in a particle analyzer |
US20110303859A1 (en) * | 2010-03-10 | 2011-12-15 | Lofstrom Christopher D | Generating Pulse Parameters in a Particle Analyzer |
US8817115B1 (en) | 2010-05-05 | 2014-08-26 | Amnis Corporation | Spatial alignment of image data from a multichannel detector using a reference image |
US20140291551A1 (en) * | 2011-11-19 | 2014-10-02 | Axure Technologies S.A. | Methods and systems for detecting and quantifying petroleum oil based on fluorescence |
US20130242302A1 (en) * | 2012-03-19 | 2013-09-19 | Sony Corporation | Fine particle measurement device |
US8913242B2 (en) * | 2012-03-19 | 2014-12-16 | Sony Corporation | Fine particle measurement device |
CN103323384A (zh) * | 2012-03-19 | 2013-09-25 | 索尼公司 | 微粒测量装置 |
CN103323384B (zh) * | 2012-03-19 | 2017-08-11 | 索尼公司 | 微粒测量装置 |
US10324020B2 (en) * | 2013-12-23 | 2019-06-18 | Palo Alto Research Center Incorporated | Fluidic optical cartridge |
US20150177118A1 (en) * | 2013-12-23 | 2015-06-25 | Palo Alto Research Center Incorporated | Fluidic optical cartridge |
US11169086B2 (en) * | 2015-04-10 | 2021-11-09 | Blaze Metrics, LLC | System and method for simultaneously performing multiple optical analyses of liquids and particles in a fluid |
US20160299060A1 (en) * | 2015-04-10 | 2016-10-13 | Blaze Metrics, LLC | System and method for simultaneously performing multiple optical analyses of liquids and particles in a fluid |
US10386290B2 (en) | 2017-03-31 | 2019-08-20 | Life Technologies Corporation | Apparatuses, systems and methods for imaging flow cytometry |
US10545085B2 (en) | 2017-03-31 | 2020-01-28 | Life Technologies Corporation | Apparatuses, systems and methods for imaging flow cytometry |
US10969326B2 (en) | 2017-03-31 | 2021-04-06 | Life Technologies Corporation | Apparatuses, systems and methods for imaging flow cytometry |
US11566995B2 (en) | 2017-03-31 | 2023-01-31 | Life Technologies Corporation | Apparatuses, systems and methods for imaging flow cytometry |
US11940371B2 (en) | 2017-03-31 | 2024-03-26 | Life Technologies Corporation | Apparatuses, systems and methods for imaging flow cytometry |
WO2022132667A1 (en) * | 2020-12-14 | 2022-06-23 | Life Technologies Corporation | Systems and methods for improved imaging and fluorescence in flow cytometry and other applications |
US11788949B2 (en) | 2020-12-14 | 2023-10-17 | Life Technologies Corporation | Systems and methods for improved imaging and fluorescence in flow cytometry and other applications |
Also Published As
Publication number | Publication date |
---|---|
USRE35868E (en) | 1998-08-11 |
JP3102935B2 (ja) | 2000-10-23 |
JPH05142137A (ja) | 1993-06-08 |
EP0543514A2 (en) | 1993-05-26 |
EP0543514A3 (en) | 1993-12-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5272354A (en) | Apparatus for imaging particles in a liquid flow | |
US5444527A (en) | Imaging flow cytometer for imaging and analyzing particle components in a liquid sample | |
US5159398A (en) | Flow imaging cytometer | |
US5422712A (en) | Apparatus for measuring fluorescent spectra of particles in a flow | |
US5247339A (en) | Flow imaging cytometer | |
US5247340A (en) | Flow imaging cytometer | |
US5159397A (en) | Flow imaging cytometer | |
US10184875B2 (en) | Apparatus and method for determining the particle size and/or the particle shape of particles in a particle stream | |
US3586760A (en) | Color television camera generating uniform lag color component signals | |
CA2111200C (en) | Electronic high-speed camera | |
JP6547073B2 (ja) | 改善されたオートフォーカス性能を有する撮像装置 | |
JPS58166244A (ja) | X線検査装置 | |
US3508051A (en) | Employing a plurality of dichroic mirrors to produce a three-color image | |
JP2826449B2 (ja) | フロー式粒子画像解析方法およびフロー式粒子画像解析装置 | |
US7067836B2 (en) | Confocal scanner system and method | |
JPS6175318A (ja) | 異なる波長の光学像を処理する装置 | |
GB2222266A (en) | Light emission for focus detection | |
JP3125045B2 (ja) | 電子線強度測定装置および電子顕微鏡 | |
JPH0918772A (ja) | 顕微鏡画像解析装置 | |
SU270495A1 (ru) | Способ цветной транспарантной киносъел\ки | |
RU200937U1 (ru) | Прибор ночного видения - дальномер с цветным изображением | |
SU195870A1 (ja) | ||
Zaytseva | Integral and spectral sensitivity assessment of the active-pulse television systems | |
JPH01149354A (ja) | 電子顕微鏡 | |
SU708288A1 (ru) | Устройство дл осуществлени способа фотометрировани негативов |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AS | Assignment |
Owner name: TOA MEDICAL ELECTRONICS CO., LTD., JAPAN Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:KOSAKA, TOKIHIRO;REEL/FRAME:006210/0222 Effective date: 19920630 |
|
STCF | Information on status: patent grant |
Free format text: PATENTED CASE |
|
CC | Certificate of correction | ||
FEPP | Fee payment procedure |
Free format text: PAYOR NUMBER ASSIGNED (ORIGINAL EVENT CODE: ASPN); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |
|
RF | Reissue application filed |
Effective date: 19951130 |
|
FPAY | Fee payment |
Year of fee payment: 4 |