US4427773A - Method for producing L-glutamic acid by fermentation - Google Patents

Method for producing L-glutamic acid by fermentation Download PDF

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US4427773A
US4427773A US06/255,169 US25516981A US4427773A US 4427773 A US4427773 A US 4427773A US 25516981 A US25516981 A US 25516981A US 4427773 A US4427773 A US 4427773A
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glutamic acid
dna
nrrl
corynebacterium
brevibacterium
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Takayasu Tsuchida
Kiyoshi Miwa
Shigeru Nakamori
Haruo Momose
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Ajinomoto Co Inc
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Ajinomoto Co Inc
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Priority claimed from JP5100180A external-priority patent/JPS56148295A/ja
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Assigned to AJINOMOTO COMPANY INCORPORATED reassignment AJINOMOTO COMPANY INCORPORATED ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: MIWA, KIYOSHI, MOMOSE, HARUO, NAKAMORI, SHIGERU, TSUCHIDA, TAKAYASU
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/13—Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/84—Brevibacterium
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/843—Corynebacterium

Definitions

  • the present invention relates to a method for producing L-glutamic acid by fermentation.
  • L-glutamic acid producing bacterial wild strains are known especially in the genus Brevibacterium or Corynebacterium.
  • artificial mutation is given to the wild strains.
  • mutants of Brevibacterium resistant to S-2-amino-ethyl-cysteine Japanese Unexamined Patent Application No. 126877/1975
  • mutants of Brevibacterium and Corynebacterium resistant to fluorocitric acid, ketomalonic acid, ⁇ -amino- ⁇ -hydroxyvaleric acid, DL-threoninehydroxamate, 2-amino-3-phosphopropionic acid or 5-aminolevulinic acid Japanese Unexamined Patent Application No. 89045/1979
  • mutants of Brevibacterium and Corynebacterium sensitive to lysozyme Japanese Unexamined Patent Application No.
  • the DNA donor used to construct the L-glutamic acid producer of this invention is an L-glutamic acid producing bacterium of the genus Brevibacterium or Corynebacterium.
  • Examples of wild strains of such L-glutamic acid producing bacteria are:
  • Brevibacterium roseum ATCC 13825
  • Corynebacterium acetoacidophilum ATCC 13870
  • Corynebacterium melassecola ATCC 17965
  • the recipients are wild or mutant strains of the genus Brevibacterium or Corynebacterium. Especially, it is convenient to use L-glutamic acid requiring mutant to select hybrid clones transformed to produce L-glutamic acid. Wild or mutant strains having higher productivity of L-glutamic acid is desirable as the recipient. In the case where L-glutamic acid requiring mutant is used as the recipient, desirably the mutant is induced from a parent strain having higher productivity of L-glutamic acid.
  • a nutrient such as L-lysine, L-threonine, L-isoleucine, L-proline, L-arginine, L-methionine, L-histidine, L-leucine, L-tryptophan, L-tyrosine, L-phenylalanine, L-alanine, L-serine, glycine, Xanthine, hypoxanthine, adenine or guanine, is given to the L-glutamic acid producing bacterium by mutation.
  • a nutrient such as L-lysine, L-threonine, L-isoleucine, L-proline, L-arginine, L-methionine, L-histidine, L-leucine, L-tryptophan, L-tyrosine, L-phenylalanine, L-alanine, L-serine, glycine, Xanthine, hypoxanthine, adenine or guanine
  • L-Glutamic acid productivity is also increased by giving to the L-glutamic acid producing bacterium sensitivity to high temperature or to polyoxysorbitane-mono-palmitate, or resistance to monofluoro-acetic acid, ketomalonic acid, guanidine, sulfaguanidine, 2-thiazolealanine or fluorophenylalanine, as is known.
  • Chromosomal DNA is extracted from the DNA donor in a well-known manner and treated with a restriction endonuclease by a well-known method (Biochem. Biophys. Acta 383: 457 (1975)). Although various kinds of restriction endonuclease are applicable if the digestion is made partly. According to the inventors findings, Hind III, Bcl I, Xba I and Xma I are most preferred for the digestion purpose.
  • vector DNA plasmid or phage DNA extracted from the L-glutamic acid producing bacteria of the genus Brevibacterium or Corynebacterium, or derivative of the plasmid or phage is used.
  • the vector DNA is digested also with restriction endonuclease.
  • Preferred restriction endonuclease are Hind III, Bcl I, Xba I and Xma I.
  • the digested chromosomal and vector DNAs are subjected to a ligation reaction with ligase.
  • Recombination of DNA to prepare the recombinant plasmid can be carried out by incorporating with terminal transferase deoxyadenylic acid and thymidylic acid, or deoxyguanylic acid and deoxycytidylic acid into the chromosomal DNA fragment and cleaved vector DNA, and by subjecting the modified chromosomal DNA fragment and cleaved DNA to an annealing reaction.
  • the hybrid DNA thus obtained can be incorporated into the recipient microorganism by conventional transformation techniques, and the recipients are thereafter allowed to grow for a while to make the transformed characteristics of transformant stable.
  • Desired transformant can be selected by screening clone having both or one of the characteristics of L-glutamic acid productivity and the characteristics possessed by the vector.
  • the L-glutamic acid producing bacteria thus obtained can be cultured by conventional manner to let it produce L-glutamic acid, such as at a pH of 6 to 8, and a temperature of 30° to 37° C. The cultivation is continued until the production of L-glutamic acid substantially ceases.
  • the culture medium employed is conventional and contains carbon source, nitrogen source, inorganic ions and when required minor organic nutrient.
  • carbon source glucose, sucrose and crude materials containing these carbohydrates (such as starch hydrolysate and molasses), organic acid such as acetic acid, and alcohol such as ethanol.
  • Gaseous ammonia, aqueous ammonia, ammonium salts and urea can be used as the nitrogen source.
  • Brevibacterium lactofermentum No. 5116 (NRRL B-12405), a mutant sensitive to a high temperature and induced from strain No. 2256 (ATCC 13869), was cultured at 30° C. for 3 hours with shaking in 1 l of CMG-medium containing 1 g/dl peptone 1 g/dl yeast extract, 0.5 g/dl glucose and 0.5 g/dl NaCl (pH was adjusted to 7.2), and bacterial cells in the exponential growth phase were harvested. Chromosomal DNA was extracted by a conventional phenol-method, and 3.5 mg of purified DNA was obtained.
  • the DNA of plasmid pAM330 (M.W. 3 ⁇ 10 6 dalton) was prepared as follows:
  • a strain of Brevibacterium lactofermentum No. 2256 harboring the plasmid pAM 330 was incubated at 30° C. in 1 l of CMG-medium. After the strain was incubated until the late log phase, the cells were harvested and then lysed by treatment with lysozyme and SDS. The lysate was centrifuged at 30,000 Xg for 30 minutes to obtain a supernatant. After concentrating the supernatant, 74 ⁇ g of the plasmid DNA was obtained by fractionation using agarose gel electrophoresis.
  • Ten ⁇ g of the chromosomal DNA was treated with each of the restriction endonucleases Hind III or Bcl 1 at 37° C. for 10, 30 and 60 minutes respectively, to cleave DNA chains, and then was heated at 65° C. for 5 minutes, respectively.
  • Ten ⁇ g of the vector DNA was also treated with each of the restriction endonucleases, Hind III or Bcl I at 37° C. for 1 hour to cleave the DNA completely, and then was heated at 65° C. for 5 minutes, respectively.
  • the digested chromosomal DNA solution and the cleaved vector DNA solution were mixed and subjected to the ligation reaction of DNA fragments by a T 4 phage DNA-ligase in the presence of ATP and dithiothreitol at 10° C. for 24 hours.
  • the reaction mixture was then heated at 65° C. for 5 minutes, and two fold volumes of ethanol were added to it.
  • the recombinant DNA which precipitated was recovered.
  • step (3) Into the competent cell suspension, the DNA obtained in step (3), which contains the hybrid plasmid DNA, was added. The suspension was kept in an ice-bath for 30 minutes, then heated at 42° C. for 2 minutes, and again allowed to stand in an ice-bath for 30 minutes. The cells, thus containing the hybrid plasmid DNA, were inoculated into an L-medium and the medium was shaken at 37° C. for 3 hours, whereby the transformation reaction was completed. The cells were harvested, washed, and resuspended.
  • a small portion of the cell suspension was spread on an agar plate containing, 20 g glucose, 10 g (NH 4 ) 2 SO 4 , 2.5 g urea, 1 g KH 2 PO 4 , 0.4 g MgSO 4 .7H 2 O, 50 ⁇ g biotin, 200 ⁇ g thiamine hydrochloride, 0.01 g FeSO 4 .7H 2 O, 0.01 g MnSO 4 .4H 2 O and 20 agar, per liter, (pH was adjusted to 7.2 ).
  • the plate was incubated at 37° C. After 4 days incubation, all of the colonies which appeared were picked up, purified and isolated.
  • the fermentation medium contained 3.6 g/dl glucose, 0.5 g/dl urea, 0.1 g KH 2 PO 4 , 0.1 g/dl MgSO 4 .7H 2 O, 3 ml/dl soybean hydrolysate ("Mieki"), 100 ⁇ g/l thiamine.HCl 3 ⁇ g/l biotin, 1 mg/dl FeSO 4 .7H 2 O, 1 mg/dl MnSO 4 .4H 2 O and 2.5 g/dl CaCO 3 (separately sterilized) and the pH was adjusted to 7.0.
  • the amounts of L-glutamic acid in the supernatant of the fermentation broth were determined by enzymatic assay.
  • Corynebacterium glutamicum No. 5707 (NRRLB-12410), a mutant resistant to ketomalonic acid and induced from Corynebacterium glutamicum AJ 11560 (FERM-P 5485) (NRRL B-12415), was cultured at 30° C. for 3 hours with shaking in 1 l of CMG-medium containing 1 g/dl peptone, 1 g/dl yeast extract, 0.5 g/dl glucose and 0.5 g/dl NaCl (pH was adjusted to 7.2), and bacterial cells in the exponential growth phase were harvested. Chromosomal DNA was extracted by a conventional phenol-method, and 4.0 mg of purified DNA was obtained.
  • Corynebacterium glutamicum AJ 11560 was newly isolated as a suitable strain for the purpose of this invention.
  • the DNA of plasmid PAM286 (M.W. 3 ⁇ 10 6 dalton) was prepared as follows:
  • a strain of Corynebacterium glutamicum AJ 11560 harboring the plasmid pAM286 was incubated at 30° C. in 1 l of CMG-medium. After the strain was incubated until the late log phase, the cells were harvested and then lysed by treatment with lysozyme and SDS. The lysate was centrifuged at 30,000 Xg for 30 minutes to obtain a supernatant. After concentrating the supernatant, 60 ⁇ g of the plasmid DNA was obtained by fractionation using agarose gel electrophoresis.
  • the digested chromosomal DNA solution and the cleaved vector DNA solution were mixed and subjected to the ligation reaction of DNA fragments by a T 4 phage DNA-ligase in the presence of ATP and dithiothreitol at 10° C. for 24 hours.
  • the reacton mixture was then heated at 65° C. for 5 minutes, and two fold volumes of ethanol were added to it.
  • the recombinant DNA which precipitated was recovered.
  • step (3) Into the competent cell suspension, the DNA obtained in step (3), which contains the hybrid plasmid DNA, was added. The suspension was kept in an ice-bath for 30 minutes, then heated at 42° C. for 2 minutes, and again allowed to stand in an ice-bath for 30 minutes. The cells, thus containing the hybrid plasmid DNA, were inoculated into an L-medium and the medium was shaken at 37° C. for 3 hours, whereby the transformation reaction was completed. The cells were harvested, washed, and resuspended.
  • the reaction mixture after having been diluted, of the cell suspension was spread on an agar plate containing, 20 g glucose, 10 g (NH 4 ) 2 SO 4 , 2.5 g urea, 1 g KH 2 PO 4 , 0.4 g MgSO 4 .7H 2 O, 50 ⁇ g biotin, 20 ⁇ g thiamine hydrochloride, 0.01 g FeSO 4 .7H 2 O, 0.01 g MnSO 4 .4H 2 O and 20 g agar, per liter, (pH was adjusted to 7.0).
  • the plate was incubated at 37° C. After 4 days incubation, all of the colonies which appeared were picked up, purified and isolated.
  • the transformants obtained in step (4) were cultured to test their L-glutamic acid productivity.
  • the DNA-donor strain No. 5707 and the recipients strains were cultured in the same manner for comparison.
  • the culture medium contained 3.6 g/dl glucose, 0.5 g/dl urea, 0.1 g KH 2 PO 4 , 0.1 g/dl MgSO 4 .7H 2 O, 3 ml/dl soybean hydrolysate ("Mieki"), 100 ⁇ g/l thiamine.HCl 3 ⁇ g/l biotin, 1 mg/dl FeSO 4 .7H 2 O, 1 mg/dl MnSO 4 .4H 2 O and 2.5 g/dl CaCO 3 (separately sterilized) and the pH was adjusted to 7.0.
  • the amounts of L-glutamic acid in the supernatant of the fermentation broth were determined by enzymatic assay.

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US06/255,169 1980-04-17 1981-04-17 Method for producing L-glutamic acid by fermentation Expired - Lifetime US4427773A (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP55-51001 1980-04-17
JP5100280A JPS56148296A (en) 1980-04-17 1980-04-17 Preparation of l-glutamic acid by fermentation method
JP5100180A JPS56148295A (en) 1980-04-17 1980-04-17 Preparation of l-glutamic acid by fermentation method
JP55-51002 1980-04-17

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US06/434,853 Continuation-In-Part US4778762A (en) 1980-04-17 1982-10-18 Plasmid

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US4427773A true US4427773A (en) 1984-01-24

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US (1) US4427773A (enrdf_load_stackoverflow)
DE (1) DE3115515A1 (enrdf_load_stackoverflow)
FR (1) FR2482133A1 (enrdf_load_stackoverflow)
GB (1) GB2076853B (enrdf_load_stackoverflow)
IT (1) IT1136577B (enrdf_load_stackoverflow)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4514502A (en) * 1982-05-04 1985-04-30 Ajinomoto Co., Inc. Composite plasmid
US4778762A (en) * 1980-04-17 1988-10-18 Ajinomoto Company Incorporated Plasmid
US5236831A (en) * 1981-12-29 1993-08-17 Kiowa Hakko Kogyo Co., Ltd. Amino acid synthesis in corynebacteria using E. coli genes
US20030119153A1 (en) * 1998-03-18 2003-06-26 Ajinomoto Co. Inc. L-glutamic acid-producing bacterium and method for producing L-glutamic acid
RU2225881C2 (ru) * 1998-03-18 2004-03-20 Адзиномото Ко., Инк. Способ получения l-глутаминовой кислоты
CN113444655A (zh) * 2020-03-26 2021-09-28 吉林中粮生化有限公司 谷氨酸棒状杆菌、高谷氨酸产量的温度敏感型菌株及其获得方法、应用以及谷氨酸发酵方法

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57183799A (en) * 1981-04-17 1982-11-12 Kyowa Hakko Kogyo Co Ltd Novel plasmid
JPS57186492A (en) * 1981-04-17 1982-11-16 Kyowa Hakko Kogyo Co Ltd Transformation of bacterium
JPS5835197A (ja) * 1981-08-26 1983-03-01 Kyowa Hakko Kogyo Co Ltd プラスミドｐｃｇ２
JPS5867699A (ja) * 1981-10-16 1983-04-22 Ajinomoto Co Inc プラスミド
IL67510A (en) * 1981-12-17 1988-08-31 Kyowa Hakko Kogyo Kk Recombinant vector plasmids autonomously replicable in microorganisms belonging to the genus corynebacterium or brevibacterium and process for the production thereof
JPS58126789A (ja) * 1981-12-29 1983-07-28 Kyowa Hakko Kogyo Co Ltd レースレオニンの製造法
US4594323A (en) * 1982-09-22 1986-06-10 The Regents Of The University Of California Hybrid DNA conferring osmotic tolerance
US4559308A (en) * 1983-01-27 1985-12-17 Stauffer Chemical Co. Corynebacterium plasmid and vector
DE3484378D1 (de) * 1983-02-17 1991-05-08 Kyowa Hakko Kogyo Kk Herstellungsverfahren fuer l-histidin.
JPS6066989A (ja) * 1983-09-24 1985-04-17 Kyowa Hakko Kogyo Co Ltd Ｌ−アルギニンの製造法
JPS59156292A (ja) * 1983-02-17 1984-09-05 Kyowa Hakko Kogyo Co Ltd トリプトフアンの製造法
JPH0732710B2 (ja) * 1983-05-28 1995-04-12 協和醗酵工業株式会社 フエニ−ルアラニンの製造法
JPS59156294A (ja) * 1983-02-17 1984-09-05 Kyowa Hakko Kogyo Co Ltd ヒスチジンの製造法
JPH0783714B2 (ja) * 1983-08-29 1995-09-13 味の素株式会社 発酵法によるｌ―アミノ酸の製造法
JPS6066984A (ja) * 1983-09-22 1985-04-17 Ajinomoto Co Inc 発酵法によるｌ−フェニルアラニンの製造法
EP0169377B1 (en) * 1984-06-22 1992-02-05 Ajinomoto Co., Inc. Plasmid and bacteria containing the same
FR2575492B1 (fr) * 1984-12-27 1988-09-16 Asahi Chemical Ind Fragment d'adn contenant un gene codant pour la glutamate deshydrogenase, adn recombinant le contenant, et microorganisme contenant l'adn recombinant
FR2590592B1 (fr) * 1985-11-26 1989-12-22 Asahi Chemical Ind Fragment d'adn contenant un gene codant pour une enzyme catalysant une reaction dans le cycle tca et adn recombinant le contenant.
JPS63273469A (ja) * 1986-12-13 1988-11-10 Kyowa Hakko Kogyo Co Ltd 乳糖資化性を有する新規微生物

Citations (1)

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US4278765A (en) 1978-06-30 1981-07-14 Debabov Vladimir G Method for preparing strains which produce aminoacids

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FR2464299A1 (fr) * 1979-08-28 1981-03-06 Inst Genetiki Selektsii Procede de preparation de souches productrices d'acides amines

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
US4278765A (en) 1978-06-30 1981-07-14 Debabov Vladimir G Method for preparing strains which produce aminoacids

Non-Patent Citations (1)

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Chakrabarty, Genetic Engineering, C.R.C. Press, Inc., Palm Beach, 1978, pp. 101 and 105.

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4778762A (en) * 1980-04-17 1988-10-18 Ajinomoto Company Incorporated Plasmid
US5236831A (en) * 1981-12-29 1993-08-17 Kiowa Hakko Kogyo Co., Ltd. Amino acid synthesis in corynebacteria using E. coli genes
US4514502A (en) * 1982-05-04 1985-04-30 Ajinomoto Co., Inc. Composite plasmid
US20030119153A1 (en) * 1998-03-18 2003-06-26 Ajinomoto Co. Inc. L-glutamic acid-producing bacterium and method for producing L-glutamic acid
RU2225881C2 (ru) * 1998-03-18 2004-03-20 Адзиномото Ко., Инк. Способ получения l-глутаминовой кислоты
US20090263874A1 (en) * 1998-03-18 2009-10-22 Ajinomoto Co. Inc L-glutamic acid-producing bacterium and method for producing l-glutamic acid
US8129151B2 (en) 1998-03-18 2012-03-06 Ajinomoto Co., Inc. L-glutamic acid-producing bacterium and method for producing L-glutamic acid
CN113444655A (zh) * 2020-03-26 2021-09-28 吉林中粮生化有限公司 谷氨酸棒状杆菌、高谷氨酸产量的温度敏感型菌株及其获得方法、应用以及谷氨酸发酵方法
CN113444655B (zh) * 2020-03-26 2023-05-16 吉林中粮生化有限公司 谷氨酸棒状杆菌、高谷氨酸产量的温度敏感型菌株及其获得方法、应用以及谷氨酸发酵方法

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FR2482133A1 (fr) 1981-11-13
DE3115515A1 (de) 1982-05-27
IT1136577B (it) 1986-09-03
GB2076853B (en) 1983-12-21
IT8121232A0 (it) 1981-04-16
FR2482133B1 (enrdf_load_stackoverflow) 1983-12-02
GB2076853A (en) 1981-12-09

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