US3927682A - Air-curing process of tobacco leaf - Google Patents
Air-curing process of tobacco leaf Download PDFInfo
- Publication number
- US3927682A US3927682A US511162A US51116274A US3927682A US 3927682 A US3927682 A US 3927682A US 511162 A US511162 A US 511162A US 51116274 A US51116274 A US 51116274A US 3927682 A US3927682 A US 3927682A
- Authority
- US
- United States
- Prior art keywords
- leaf
- tobacco
- air
- bruised
- bruising
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 42
- 235000002637 Nicotiana tabacum Nutrition 0.000 title abstract description 82
- 241000208125 Nicotiana Species 0.000 title abstract description 72
- 235000019506 cigar Nutrition 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 208000034656 Contusions Diseases 0.000 claims description 12
- 238000004383 yellowing Methods 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 11
- 238000005273 aeration Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 238000006911 enzymatic reaction Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 abstract description 7
- 238000002156 mixing Methods 0.000 abstract description 6
- 235000019505 tobacco product Nutrition 0.000 abstract description 6
- 230000015572 biosynthetic process Effects 0.000 abstract description 5
- 239000000470 constituent Substances 0.000 abstract description 5
- 230000002255 enzymatic effect Effects 0.000 abstract description 2
- 230000003647 oxidation Effects 0.000 abstract description 2
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- 238000001723 curing Methods 0.000 description 22
- 244000061176 Nicotiana tabacum Species 0.000 description 11
- 235000019504 cigarettes Nutrition 0.000 description 8
- 230000000391 smoking effect Effects 0.000 description 8
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 4
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 4
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 4
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 4
- 229940074393 chlorogenic acid Drugs 0.000 description 4
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 4
- 235000001368 chlorogenic acid Nutrition 0.000 description 4
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 4
- 238000000354 decomposition reaction Methods 0.000 description 4
- 229910017464 nitrogen compound Inorganic materials 0.000 description 4
- 150000002830 nitrogen compounds Chemical class 0.000 description 4
- 230000001953 sensory effect Effects 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 150000001720 carbohydrates Chemical class 0.000 description 3
- 102000030523 Catechol oxidase Human genes 0.000 description 2
- 108010031396 Catechol oxidase Proteins 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 206010013911 Dysgeusia Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000410737 Verasper moseri Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical group 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- JGPMMRGNQUBGND-UHFFFAOYSA-N idebenone Chemical compound COC1=C(OC)C(=O)C(CCCCCCCCCCO)=C(C)C1=O JGPMMRGNQUBGND-UHFFFAOYSA-N 0.000 description 1
- 229960004135 idebenone Drugs 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
Definitions
- the curing process of curing the tobacco leaf is essentially a complex operation in which the constituents of green leaf are caused to change gradually either by the action of the enzyme in the leaf or by oxygen in the open air so as to adapt them to smoking (this change is called curing); the moisture in the green leaf is caused to decrease gradually or step by step (such reduction of the moisture is called drying).
- the courses of the curing process of the tobacco leaf consist concretely of several stages of so called pre-drying, pre-yellowing, yellowing, (browning,) color-fixing and midrib-drying.
- Tobacco leaf may be classified generally into two types, 1 Nicotiana tabacum var. bright yellow or the like which contains a larger amount of carbohydrate but a smaller amount of nitrogen compound, and 2 N. tabacum 'var. burley 21, or the like, which contains a smaller amount of carbohydrate but a larger amount of nitrogen compound.
- the fonner type of tobacco leaf there is applied the so-called flue-cure in which the product is finished in a state of containing a comparatively larger amount of sugar
- the so-called aircure in which the product is finished in a state of a large part of the nitrogen compound contained in the green leaf having been decomposed.
- the reason for such specialization of the curing process of tobacco leaf is that, the said nitrogen compound contained in the leaf largely affects the aroma and taste of the tobacco product so that the decomposition of said compound has been regarded as the most essential matter in the cure of tobacco leaf, and further that, in order to improve the aroma and taste, the bulkiness and the burning quality of the cigarette, the form of which is adopted most widely as a convenient form of smoking tobacco at present, it is regarded as most suitable that the blending of said flue-cured leaf and air-cured leaf, both have been so' made since they exhibit their respective characters so significantly.
- the air-cure which is applied to burleytobacco leaf, domestic tobacco leaf, cigar tobacco leaf and others it is necessary to carry out the cure spending a longer period at lower temperatures in comparison with those in the case of the flue-cure.
- the period required for the stages of browning and midrib-drying in the air-curing process, which stages succeed the yellowing stage amounts to 7-15 days, and thus the total period of the same process takes 10-20 days.
- An object of the invention is accordingly to provide a process of air-curing tobacco leaf wherein the browning and formation of smoking aroma and taste thereof are attained within a remarkably shorter period in comparison with that in the conventional process.
- Another object of the invention is to provide a process of air-curving tobacco leaf as a result of which the tobacco product has excellent aroma and taste in comparison with that generated from the conventional process.
- Still another object of the invention is to provide a process of air-curing tobacco leaf wherein an uniform browning and formation of aroma and taste of the tobacco leaf may be attained finally even in the case of non uniform yellowing which has been caused in the preceding stage.
- the harvested leaf of burley tobacco, domestic tobacco or cigar tobacco (these tobaccos being called for short tobacco hereinafter) is caused to yellow by being treated with the routine manner. That is, for example, tobacco leaf with or without the stalk is subjected to the successive operations of pre-drying, pre-yellowing and yellowing in order, and the moisture content of the leaf is reduced thereby to about 40-70 percent by wet basis.
- the moisture content in the above tobacco leaf is far lower in comparison with that in the leaf at the same stage of the conventional air-curing process, and such lower moisture content is developed only in relation to the subsequent stage, in which the yellowed leaf is bruised mechanically.
- the carbohydrate material such as starch in the tobacco leaf is hydrolyzed into soluble sugar by amylase and the like, the protein is decomposed into soluble amino acid by protease and the like; and the decomposition of fatty acid and of chlorophyl are promoted. Furthermore, the activity of oxidizing enzymes such as polyphenol oxidase and peroxidase is enchanced in this stage.
- the tobacco leaf which has been yellowed and the constituents of which have been converted as described above is subjected to bruising mechanically so as to cause the sap of the leaf cells to transude and to come in contact with the open air, and thereb the browning reaction of the tobacco leaf is uniformly promoted as a whole.
- the said mechanical bruising of the tobacco leaf may be accomplished either by crushing of the yellowed leaf in a kneader or the like or by Several times passing the leaf several times through pairs of pressing rollers.
- Such bruising operation is carried out at least to such an extent that the whole of the leaf tissue contains moisture uniformly and appears as if water has soaked therein (in other words to such an extent that either the volume of the gas contained in the leaf is reduced to about half of its initial volume or until the leaf turns translucent).
- the specific time required for the bruising operation on the tobacco leaf is from a few minutes to 2 hours, varying with the character of the leaf to be treated, apparatus used, temperature and other factors.
- the tobacco leaf having been bruised as described above is allowed, if necessary, to stand under the condition of from room temperature to 45C and relative humidity of 70-90 percent in the state of being put one upon another in a thin layer for 24 hours at most, and is kept at a temperature of 90-l30C for a short time (e.g., 30 minutes) so as to cause the enzymatic reaction in the leaf to stop, followed by drying at 5060C while aeration is carried out. After completion of the aeration-drying, the leaf is moisture-conditioned if necessary, and is either shredded or pulverized to make it into raw material for sheet tobacco.
- the raw material for tobacco produced by the process of the invention has a concentrated aroma and taste and exhibits a brilliant brown, and the blended tobacco product which is made by mixing a small amount of said material with another tobacco material produced by the flue-cure, is recognized, through sensory test, to be such as to be capable of giving a smoking aroma and taste peculiar to the kind (species) of tobacco leaf which has been employed in the process of the invention.
- Tobacco plants belonging to Nicotiana tabacum to which the air-curing process of the present invention may be applied are as follows:
- burley tobacco cv. such as N.t. var. burley 21, N.t. var. burley mito 3, N.t. var. burley ky l and the like; domestic (Japanese) tobacco cv. such as N.t. var. suifu, N.t. var. matsukawa, N.t. var. daruma, N.t. var. ibusuki, N.t. var. maru, N.t. var. bitchu, N.t. var hatano, N.t. var. kirigasaku, N.t. var. enshu, N.t. var. awa, N.t. var. nambu, N.t. var. shiroenshu and the like; and cigar tobacco cv. such as N.t. var. florida, N.t. var. dixie shade, N.t. var. havana and the like.
- Example 1 Smoking leaf of Nicotiana tabacum var. burley 21 is harvested and pre-dried, followed by hanging the tobacco in a barn having a temperature of 30C and a relative humidity (RH) of 60 percent for about 3 days so as to cause yellowing together with dehydration.
- RH relative humidity
- the leaf Upon the moisture content being reduced to about 50 percent (w.b.), the leaf is placed in a kneader equipped with a double paddle and crushed thereby for an hour. The crushed leaf is then placed in a dryer at a temperature of 100C for 30 minutes, and thereafter dried at 60C for 4 hours while carrying out aeration.
- the prodnot having been dried in this way is moisture-conditioned, and then, without having been shredded, it is made into a cigarette.
- the amount of chlorogenic acid in the leaf at each stage of the present Example 1 is compared with that of the same acid in the leaf at the corresponding stage of the conventional air-curing process, said amount of chlorogenic acid being regarded generally as an indicator of the degree of progress in the browning of tobacco leaf.
- the results of the test are shown in Table 1 below, from which it is seen that in the process of the invention the browning of the leaf is accompanied with the conversion of the constitutents of the leaf.
- a sensory test is carried out, wherein a comparison is made between (a) the cigarette made of the blended product which is composed of 10 percent of the burley tobacco prepared by this Example 1 of the present invention and of 90 percent of the shredded tobacco prepared by the flue-curing process, and (b) the cigarette made of the blended product which is composed of 20 percent of the shredded burley tobacco prepared by the conventional air-curing process using the same kind of tobacco leaf and of percent of the said shredded tobacco prepared by the flue-curing process.
- Table 3 wherein this numbers have the same meaning as that in Table 2 except that a panel consisting of 13 professional persons is employed therein, instead of that consisting of 10 professional persons in the case of Table 2.
- EXAMPLE 2 Smoking leaf of Nicotiana tabacum var. burley 21 is harvested and predried, followed by hanging in a barn at 30 C and at 60 percent RH for 88 hours so as to cause yellowing and to reduce the moisture content of the leaf to about 50 percent (w.b.).
- the yellowed leaf is crushed with a kneader having a double paddle for an hour, and the crushed leaf is then placed in a dryer at a temperature of 100 C for 30 minutes, followed by drying at 50 C for 4.5 hours while aerating the leaf.
- the product having been dried in this way is moistureconditioned, made into a cigarette and the cigarette is submitted to the sensory test, above described. Thereby, it is observed that the present product has the essential aroma of burley tobacco as well as physiological lightness.
- EXAMPLE 3 Smoking leaf of Nicotiana tabacum var. burley 21 is harvested and predried, followed by standing at room temperature for 90 hours so as to cause yellowing and to cause the moisture content of be the leaf to 60-70 percent.
- This yellowed leaf is subjected to a process of passing it through 3 pairs of rollers each of which is covered with rubber-sheet, in order: The said pair of neighboring rollers is operated with a closing pressure of 3 kg/cm and eachof said rollers has a diameter of 10 cm and is operated at a rate of 30 rpm. Thereby, the midribs of the leaf are crushed and the whole tissue of the leaf body appears to have water uniformly soaked therein.
- step (A) bruising the yellowed leaf in step (A) mechanically to such an extent that the whole of the leaf tissue contains moisture uniformly and appears as if water has soaked therein,
- step (B) heating the bruised leaf in step (B) at a temperature of 90l30 C so as to stop the enzymatic reaction in the leaf
- step (C) drying the leaf treated in step (C) at 506O C while aeration is carried out.
Landscapes
- Manufacture Of Tobacco Products (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP48120392A JPS5231959B2 (enrdf_load_stackoverflow) | 1973-10-27 | 1973-10-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
US3927682A true US3927682A (en) | 1975-12-23 |
Family
ID=14785058
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US511162A Expired - Lifetime US3927682A (en) | 1973-10-27 | 1974-10-02 | Air-curing process of tobacco leaf |
Country Status (3)
Country | Link |
---|---|
US (1) | US3927682A (enrdf_load_stackoverflow) |
JP (1) | JPS5231959B2 (enrdf_load_stackoverflow) |
BR (1) | BR7408948D0 (enrdf_load_stackoverflow) |
Cited By (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000002464A1 (en) * | 1998-07-08 | 2000-01-20 | Novozymes A/S | Use of a phenol oxidising enzyme in the treatment of tobacco |
US6298859B1 (en) * | 1998-07-08 | 2001-10-09 | Novozymes A/S | Use of a phenol oxidizing enzyme in the treatment of tobacco |
WO2012125245A1 (en) * | 2011-03-15 | 2012-09-20 | R.J. Reynolds Tobacco Company | Cured tobacco and method therefor |
CN102715632A (zh) * | 2012-06-20 | 2012-10-10 | 湖北中烟工业有限责任公司 | 一种降低白肋烟中有害成分的调制工艺 |
EP2609817A1 (en) * | 2011-12-30 | 2013-07-03 | Philip Morris Products S.A. | Method for flattening tobacco leaves |
CN103271428A (zh) * | 2013-05-06 | 2013-09-04 | 广东省烟草南雄科学研究所 | 一种烤烟高温逼熟烟叶的精准密集烘烤工艺 |
CN103284303A (zh) * | 2013-04-16 | 2013-09-11 | 川渝中烟工业有限责任公司 | 采用滚筒管板式烘丝机降低卷烟氢氰酸释放量的烘丝方法 |
CN103284302A (zh) * | 2013-04-16 | 2013-09-11 | 川渝中烟工业有限责任公司 | 采用滚筒管板式烘丝机降低卷烟h值的烘丝方法 |
CN103300468A (zh) * | 2012-03-17 | 2013-09-18 | 福建中烟工业有限责任公司 | 一种用于香料烟烘焙的加料香精及其制备方法 |
CN103653228A (zh) * | 2012-09-25 | 2014-03-26 | 姜永辉 | 全自动流水线烤烟房及烟叶烘烤方法 |
CN103919266A (zh) * | 2014-04-24 | 2014-07-16 | 遵义市烟草公司余庆县分公司 | 一种烤烟烘烤提速增香方法 |
US20140352706A1 (en) * | 2011-09-02 | 2014-12-04 | Philip Morris Products S.A. | Isopropylmalate synthase from nicotiana tabacum and methods and uses thereof |
CN104509964A (zh) * | 2013-09-26 | 2015-04-15 | 贵州省烟草科学研究院 | 一种“两长一短、低湿慢烤”的烤烟烘烤工艺 |
US20150173319A1 (en) * | 2013-12-06 | 2015-06-25 | Altria Client Services Inc. | Tobacco plants having altered amounts of one or more alkaloids in leaf and methods of using such plants |
CN104997151A (zh) * | 2015-08-05 | 2015-10-28 | 四川省烟草公司泸州市公司(泸州市烟草公司) | 一种烤烟品种云烟99的烘烤工艺 |
CN106387975A (zh) * | 2016-09-09 | 2017-02-15 | 福建中烟工业有限责任公司 | 一种烟叶的初烤方法、烟叶及其用途 |
CN107594607A (zh) * | 2017-11-17 | 2018-01-19 | 云南省烟草农业科学研究院 | 一种稳温降湿的烤烟密集烘烤方法 |
CN108185497A (zh) * | 2018-01-18 | 2018-06-22 | 福建中烟工业有限责任公司 | 翠碧一号烟叶的初烤方法、所得的烟叶及其应用 |
CN109516640A (zh) * | 2018-12-14 | 2019-03-26 | 河南卷烟工业烟草薄片有限公司 | 造纸法再造烟叶高浓废水的预处理工艺 |
CN112741357A (zh) * | 2019-10-31 | 2021-05-04 | 中国烟草总公司海南省公司 | 一种雪茄烟叶的制备方法 |
CN112754053A (zh) * | 2019-11-01 | 2021-05-07 | 中国烟草总公司海南省公司 | 一种多层栅网栏式的雪茄烟叶晾制框 |
CN113854618A (zh) * | 2021-11-22 | 2021-12-31 | 云南省烟草农业科学研究院 | 利用人工控制提高加热卷烟土烟原料烟叶品质的调制方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US772892A (en) * | 1897-12-10 | 1904-10-18 | American Tobacco Co | Process of treating tobacco-stems, &c. |
US1401438A (en) * | 1920-08-02 | 1921-12-27 | J L Galbreth | Tobacco-leaf crusher |
US3371670A (en) * | 1966-04-18 | 1968-03-05 | Carl R. Camenisch | Apparatus for curing tobacco |
US3599645A (en) * | 1969-03-18 | 1971-08-17 | Research Corp | Treatment of tobacco to reduce polyphenol content |
US3828798A (en) * | 1972-11-20 | 1974-08-13 | Brown & Williamson Tobacco | Aromatic tobacco forming process |
-
1973
- 1973-10-27 JP JP48120392A patent/JPS5231959B2/ja not_active Expired
-
1974
- 1974-10-02 US US511162A patent/US3927682A/en not_active Expired - Lifetime
- 1974-10-25 BR BR8948/74A patent/BR7408948D0/pt unknown
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US772892A (en) * | 1897-12-10 | 1904-10-18 | American Tobacco Co | Process of treating tobacco-stems, &c. |
US1401438A (en) * | 1920-08-02 | 1921-12-27 | J L Galbreth | Tobacco-leaf crusher |
US3371670A (en) * | 1966-04-18 | 1968-03-05 | Carl R. Camenisch | Apparatus for curing tobacco |
US3599645A (en) * | 1969-03-18 | 1971-08-17 | Research Corp | Treatment of tobacco to reduce polyphenol content |
US3828798A (en) * | 1972-11-20 | 1974-08-13 | Brown & Williamson Tobacco | Aromatic tobacco forming process |
Cited By (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6298859B1 (en) * | 1998-07-08 | 2001-10-09 | Novozymes A/S | Use of a phenol oxidizing enzyme in the treatment of tobacco |
AU761885B2 (en) * | 1998-07-08 | 2003-06-12 | British American Tobacco (Investments) Limited | Use of a phenol oxidising enzyme in the treatment of tobacco |
WO2000002464A1 (en) * | 1998-07-08 | 2000-01-20 | Novozymes A/S | Use of a phenol oxidising enzyme in the treatment of tobacco |
CN103429103A (zh) * | 2011-03-15 | 2013-12-04 | R·J·雷诺兹烟草公司 | 烤制烟草和其方法 |
WO2012125245A1 (en) * | 2011-03-15 | 2012-09-20 | R.J. Reynolds Tobacco Company | Cured tobacco and method therefor |
JP2014511672A (ja) * | 2011-03-15 | 2014-05-19 | アール・ジエイ・レイノルズ・タバコ・カンパニー | 乾燥処理されたタバコ及び乾燥処理方法 |
US9066538B2 (en) | 2011-03-15 | 2015-06-30 | R.J. Reynolds Tobacco Company | Cured tobacco and method therefor |
CN103429103B (zh) * | 2011-03-15 | 2016-12-21 | R·J·雷诺兹烟草公司 | 烤制烟草和其方法 |
US10287599B2 (en) * | 2011-09-02 | 2019-05-14 | Philip Morris Products S.A. | Isopropylmalate synthase from Nicotiana tabacum and methods and uses thereof |
US20140352706A1 (en) * | 2011-09-02 | 2014-12-04 | Philip Morris Products S.A. | Isopropylmalate synthase from nicotiana tabacum and methods and uses thereof |
WO2013097949A1 (en) * | 2011-12-30 | 2013-07-04 | Philip Morris Products S.A. | Method for flattening tobacco leaves |
EP2609817A1 (en) * | 2011-12-30 | 2013-07-03 | Philip Morris Products S.A. | Method for flattening tobacco leaves |
CN103300468A (zh) * | 2012-03-17 | 2013-09-18 | 福建中烟工业有限责任公司 | 一种用于香料烟烘焙的加料香精及其制备方法 |
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JPS5070600A (enrdf_load_stackoverflow) | 1975-06-12 |
BR7408948D0 (pt) | 1975-08-26 |
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