US3780935A - Serum separating method - Google Patents

Serum separating method Download PDF

Info

Publication number
US3780935A
US3780935A US00270278A US3780935DA US3780935A US 3780935 A US3780935 A US 3780935A US 00270278 A US00270278 A US 00270278A US 3780935D A US3780935D A US 3780935DA US 3780935 A US3780935 A US 3780935A
Authority
US
United States
Prior art keywords
sealant
specific gravity
serum
sample
blood
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US00270278A
Other languages
English (en)
Inventor
M Lukacs
I Jacoby
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Organon Teknika Corp
LUKACS AND JACOBY ASS
Original Assignee
LUKACS AND JACOBY ASS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LUKACS AND JACOBY ASS filed Critical LUKACS AND JACOBY ASS
Application granted granted Critical
Publication of US3780935A publication Critical patent/US3780935A/en
Assigned to ORGANON TEKNIKA CORPORATION, A CORP. OF DE. reassignment ORGANON TEKNIKA CORPORATION, A CORP. OF DE. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: LUKACS AND JACOBY ASSOCIATES
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]

Definitions

  • FIG. 2 FIG. 3
  • This invention relates to a method, apparatus and composition of matter for separating a blood sample by centrifuging into clot portions, so that the serum may be quickly and readily removed without contamination by the clot portion.
  • the sealant has a specific gravity of at least 1.026 and preferably in the range of 1.030 to 1.050. As such, it will normally be at the proper specific gravity to divide the serum and clot portions of the centrifuged sample, sealing the clot in the container while the serum is removed.
  • FIG. 4 is a view similar to FIG. 2 wherein the blood, the sample tube and dispenser have been subjected to the complete centrifuging step;
  • FIG. 5 is a sectional view of the sample tube with the separator in place overlying the clot portion with the serum portion removed;
  • FIG. 6 is an enlarged sectional view of the sealant dispenser showing the sealant contained therein;
  • FIG. 7 is a top view of the dispenser of FIG. 6.
  • FIG. 8 is a bottom view of the dispenser of FIG. 6.
  • a container 10 for holding a blood sample is illustrated as a straight side wall sample tube 10 with an open top end 11.
  • a dispenser 12 Into the open top 11 is inserted a dispenser 12.
  • the dispenser 12 has a flange member 14 which overlies the top edge 16 of the side wall 18 of the container.
  • the dispenser 12 includes a body portion 19; consisting of three ring sections 20, 22 and 24. Extending from the body 19 is an elongated nozzle portion 26 and at the end of the nozzle 26 is a tip 28 having an opening 30 therein.
  • the purpose of the three rings 20, 22 and 24 is to permit the dispenser 12 to be used with containers of various diameters.
  • Ring 20 has a shoulder 32 and a side wall 33,-ring 22 a shoulder 34 and a side wall 35, and ring 24 has a shoulder 36 and a side wall 37.
  • the dispenser bears on the top edge of the side wall of the container at one of the shoulders 32, 34 or 36 and the side wall of the next smaller ring is parallel with the inner side wall of the container.
  • the relationship between the inner surface of the container and the side wall of the ring insures stability of the dispenser during this period.
  • the body portion 19 of the dispenser has an open end 37 over which is placed a seal 38.
  • the seal 38 has a small opening 40 therein. The seal 38 is not placed over the open end until a sealant 42 has been placed in the dispenser.
  • the sealant 42 consists essentially of a silicone fluid with an inert filler, such as silica, dispersed therein.
  • the sealant should have a specific gravity of at least 1.026
  • the normal specific gravity of blood as determined by the pycnometric method is considered to be in the range of 1.048 to 1.066 with averages of 1.052 to 1.063. After centrifuging the specific gravity of the blood serum which separates from the remainder of the blood is at least 1.026 and in the range of 1.026 to 1.031. The specific gravity of the heavier portions such as the erythrocytes is 1.092 to 1.095.
  • the sealant In selecting a sealant it is necessary to select one which has a specific gravity greater than that of the serum portion. Accordingly, the sealant should have a specific gravity of at least 1.026. However, its specific gravity should not be too high so as to cause it to layer somewhere in the clot portion. Such layering would be of no practical use towards obtaining a separated serum portion. A preferred sealant would have a specific gravity in the range of 1.030 to 1.050.
  • the sealant is also preferably thioxtropic, water insoluble, substantially non-toxic as well as substantially chemically inert with respect to the constituents of the blood sample, particularly those in the serum portion.
  • a preferred sealant formulation is as follows:
  • Example I Parts by weight
  • the silicone fluid used in Example I was a dimethylpolysiloxane polymer made by Union Carbide Corporation and identified by the designation L- 45. it had a viscosity of 12,500 centistokes and a specific gravity of about 0.973 at 25C.
  • the silica with a specific gravity of 2.65 was an amorphous silica having a particle size of at least 75 percent being less than 5.0 microns. It was made by Whittaker, Clark & Daniels and identified by the designation No. 31 Lo Micron".
  • the silica with a specific gravity of 2.3 was a hydrophobic amorphous silica having an average particle size of about 20 millimicrons. It was made by Degussa Inc. and identified by the designation Aerosil R 972.
  • the silicone fluid and the silica were mixed together to a thixotropic condition with a resultant specific gravity of 1.045 to 1.050 to form the sealant.
  • the sealant was then placed in a dispenser of the type illustrated in FIG. 6 in particular.
  • the diameter of the flange was 0.70 inches and the overall length 1.75 inches with the nozzle and tip being 1.10 inches.
  • the opening in the tip was 0.032 inches.
  • the filled dispenser was placed in a standard sample tube having an overall length of 3.875 inches with the shoulder 32 of the first ring resting on the top of the tube side wall.
  • the tube had previously been filled with a whole blood specimen to within 1.10 inches of the open end of the tube.
  • the extent of the dispenser from shoulder 32 to the end of the tip was 1.5 inches. Thus, the dispenser tip and part of the nozzle extended well into the blood sample.
  • the tube with the blood sample and dispenser was centrifuged for approximately 10 minutes. After 5 minutes substantially all of the sealant had passed from the dispenser.
  • the sealant did not disperse but instead remained homogeneous and settled as a layer between the serum and clot portions of the centrifuged blood. It was noted that the sealant settled as a substantially even layer between the two portions since its specific gravity of 1.045 to 1.050 was less than that of the clot portion and greater than that of the serum portion.
  • the sealant formed a tight seal against the inner wall of the tube. Also noted was the fact, that the sealant had mixed into it, particularly in the portion near the clot portion, fibrant matter which had been filtered out of the serum portion as the sealant settled to its own specific gravity level.
  • the specific gravity of the sealant was determined by using a copper sulfate method. The procedure consists of letting drops of the sealant fall into a graded series of solutions of copper sulfate of known specific gravity and noting whether the drops rise or fall. The series used were graded at 0.005 intervals. Merely by observing the drops it was possible to determine that the sealant had a specific gravity between 1.045 and 1.050.
  • Silicone fluid (dimethylpolysiloxane) I00 Silica (specific gravity 1.95) 14
  • the silicone fluid of Example 11 is the same dimethylpolysiloxane polymer of Example 1.
  • the silica with a specific gravity of 1.05 has an average particle size of 16 millimicrons. it is made by Henlig & Co. and identified by the designation TRI-SlL 404.
  • the specific gravity is from 1.045 to 1.050.
  • Still another sealant formulation is as follows:
  • Example 11 Parts by weight Silicone fluid (ethyltriethoxysilane) Silica (specific gravity 2.65) 8 Silica (specific gravity 2.3) 20
  • the silicone fluid of Example 11 is an ethyltriethoxysilane monomer made by Union Carbide Corporation and identified by the designation A-15.
  • the silicas are the same as those used in Example I.
  • the filler serves the dual purpose of making the silicone fluid thixotropic and of adjusting the specific gravity to that which is desired for the resultant sealant.
  • silica inert fillers which may be used in a fine powdered form are bentoniate, alumina and talc. Others will also occur to those skilled in the art.
  • silicone fluid which may be used is made by Dow Corning Corporation and identified as 200 Fluid.
  • silicone fluids and fillers one should select materials which when mixed together will give the desired specific gravity, be substantially non-toxic, water insoluble and substantially chemically inert with respect to the constituents of at least the serum portion.
  • the dispenser serves to meter out the sealant gradually and this gradual metering provides sufficient lead time for the centrifugation to take effect before the sealant is in place. If the sealant were allowed to be positioned too soon some unwanted matter, such as red cells or fibrilar like material, may be trapped in the serum portion by the sealant.
  • a method of separating a sample of whole blood into serum and clot portions comprising the steps of:
  • substantially non-toxic sealant consisting essentially of a silicone fluid and an inert filler dispersed therein, said sealant having a specific gravity in the range of 1.026 to 1.092;
  • sealant has a specific gravity in the range of 1.030 to 1.050.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
US00270278A 1972-07-10 1972-07-10 Serum separating method Expired - Lifetime US3780935A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US27027872A 1972-07-10 1972-07-10

Publications (1)

Publication Number Publication Date
US3780935A true US3780935A (en) 1973-12-25

Family

ID=23030661

Family Applications (1)

Application Number Title Priority Date Filing Date
US00270278A Expired - Lifetime US3780935A (en) 1972-07-10 1972-07-10 Serum separating method

Country Status (4)

Country Link
US (1) US3780935A (ja)
CH (1) CH611424A5 (ja)
FR (1) FR2240668A5 (ja)
GB (1) GB1443439A (ja)

Cited By (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3852194A (en) * 1972-12-11 1974-12-03 Corning Glass Works Apparatus and method for fluid collection and partitioning
US3869388A (en) * 1973-06-25 1975-03-04 Continental Oil Co Oil water separation
US3909419A (en) * 1974-02-27 1975-09-30 Becton Dickinson Co Plasma separator with squeezed sealant
US3919085A (en) * 1974-02-27 1975-11-11 Becton Dickinson Co Plasma separator assembly
US3920557A (en) * 1974-02-27 1975-11-18 Becton Dickinson Co Serum/plasma separator--beads-plus-adhesive type
US3920549A (en) * 1974-03-18 1975-11-18 Corning Glass Works Method and apparatus for multiphase fluid collection and separation
US3929646A (en) * 1974-07-22 1975-12-30 Technicon Instr Serum separator and fibrin filter
US3957653A (en) * 1975-04-03 1976-05-18 Becton, Dickinson And Company Apparatus for collection, separation and isolation of blood
US3957654A (en) * 1974-02-27 1976-05-18 Becton, Dickinson And Company Plasma separator with barrier to eject sealant
FR2295079A1 (fr) * 1974-12-16 1976-07-16 Corning Glass Works Composition stabilisee pour la separation du sang
US3976579A (en) * 1975-07-10 1976-08-24 Becton, Dickinson And Company Novel assembly
US3977982A (en) * 1975-01-20 1976-08-31 Corning Glass Works Controlling viscosity of silica-silicone oil gels
JPS51105891A (en) * 1974-01-30 1976-09-20 Wadley Res Inst & Blood Bank Enshinbunryosochi byogenseibiseibutsukenshutsuyosochi oyobi kenshutsuhoho
US3986962A (en) * 1975-07-10 1976-10-19 Becton, Dickinson And Company Novel assembly for separating blood
US4001122A (en) * 1973-08-22 1977-01-04 Telan Corporation Method and device for separating blood components
US4018564A (en) * 1975-11-24 1977-04-19 General Electric Company Silicone composition for analyzing blood samples
US4021340A (en) * 1975-11-28 1977-05-03 Corning Glass Works Blood separating composition
US4043928A (en) * 1973-10-31 1977-08-23 Lukacs And Jacoby Associates Serum separating composition of matter
US4052320A (en) * 1975-08-29 1977-10-04 Eastman Kodak Company Telescoping serum separator and dispenser
US4055501A (en) * 1976-01-16 1977-10-25 Sherwood Medical Industries Inc. Fluid collection device with phase partitioning means
DE2743882A1 (de) * 1976-09-29 1978-03-30 Nippon Chemiphar Co Trennmittel und verfahren zum auftrennen einer blutprobe in serum- oder plasma- und gerinnsel-anteile
US4088582A (en) * 1976-01-16 1978-05-09 Sherwood Medical Industries Inc. Blood phase separation means
DE2820706A1 (de) * 1977-05-11 1978-11-16 Unilever Emery Copolyesterzusammensetzung
US4152269A (en) * 1977-02-01 1979-05-01 Warner-Lambert Company Collection and separation device
US4167955A (en) * 1978-06-05 1979-09-18 Beckman Instruments, Inc. Layering a liquid sample onto centrifuge tube liquid contents
US4189382A (en) * 1974-11-07 1980-02-19 Sherwood Medical Industries Inc. Blood coagulation and separation
US4190535A (en) * 1978-02-27 1980-02-26 Corning Glass Works Means for separating lymphocytes and monocytes from anticoagulated blood
US4235725A (en) * 1978-08-16 1980-11-25 Owens-Illinois, Inc. Sterile blood-collecting and separating device
US4279863A (en) * 1979-09-12 1981-07-21 Sherwood Medical Industries, Inc. Reagent separator for a blood collection tube
EP0046391A1 (en) * 1980-08-18 1982-02-24 Sekisui Kagaku Kogyo Kabushiki Kaisha Composition for partitioning blood components
US4333564A (en) * 1978-05-22 1982-06-08 Sherwood Medical Industries Inc. Method of controlling rheological properties of gel-like compositions
US4350593A (en) * 1977-12-19 1982-09-21 Becton, Dickinson And Company Assembly, compositions and method for separating blood
US4386003A (en) * 1981-09-17 1983-05-31 Sherwood Medical Industries Inc. Blood separating composition
US4426290A (en) 1980-05-08 1984-01-17 Terumo Corporation Apparatus for separating blood
US4994393A (en) * 1989-02-22 1991-02-19 Becton, Dickinson And Company Blood partitioning composition
US5169543A (en) * 1988-07-26 1992-12-08 Nippon Paint Co., Ltd. Serum separation sealant and fine resin particles using the same
US5236604A (en) * 1991-05-29 1993-08-17 Sherwood Medical Company Serum separation blood collection tube and the method of using thereof
US5266199A (en) * 1990-11-20 1993-11-30 Nigata Chemicals And Plastics Co., Ltd. Serum separating apparatus
US5269927A (en) * 1991-05-29 1993-12-14 Sherwood Medical Company Separation device for use in blood collection tubes
US5438000A (en) * 1992-11-12 1995-08-01 Nippon Paint Co., Ltd. Serum separation sealant
US5506333A (en) * 1993-07-30 1996-04-09 Henkel Corporation Polyesters for use in blood partitioning
EP0705882A2 (en) 1994-10-05 1996-04-10 Becton, Dickinson and Company Blood compatible, shear sensitive formulations
US5968018A (en) * 1996-10-30 1999-10-19 Cohesion Corporation Cell separation device and in-line orifice mixer system
US5997811A (en) * 1997-07-02 1999-12-07 Cohesion Technologies, Inc. Method for sterile syringe packaging and handling
US6376210B1 (en) 1999-07-06 2002-04-23 General Atomics Methods and compositions for assaying analytes
US6398956B1 (en) 1999-05-28 2002-06-04 Bio/Data Corporation Method and apparatus for directly sampling a fluid for microfiltration
US6610504B1 (en) 2000-04-10 2003-08-26 General Atomics Methods of determining SAM-dependent methyltransferase activity using a mutant SAH hydrolase
US20030213312A1 (en) * 2002-05-17 2003-11-20 Bayer Corporation Serum transfer cup
US20030215389A1 (en) * 2000-10-02 2003-11-20 Anthony Montgomery Methods for detecting vasculopathies and tumors
US20040014083A1 (en) * 2000-02-25 2004-01-22 Chong-Sheng Yuan Detection of heteroduplex polynucleotides using mutant nucleic acid repair enzymes with attenuated catalytic activity
US20040072367A1 (en) * 2002-07-26 2004-04-15 Ortho-Clinical Diagnostics, Inc. Metering tip with internal features to control fluid meniscus and oscillation
US20050244912A1 (en) * 2004-04-30 2005-11-03 Chong-Sheng Yuan Methods for assaying inhibitors of S-adenosylhomocysteine (SAH) hydrolase and S-adenosylmethionine (SAM)-dependent methyltransferase
US20060060531A1 (en) * 1999-05-28 2006-03-23 Bio/Data Corporation Method and apparatus for directly sampling a fluid for microfiltration
US7151000B2 (en) * 1999-03-05 2006-12-19 The Regents Of The University Of California Method of concentrating proteins from serum
US7192729B2 (en) 1999-07-06 2007-03-20 General Atomics Methods for assaying homocysteine
US20070190148A1 (en) * 2006-02-14 2007-08-16 Peter Cronin Gel compositions, apparatuses and fluid separation methods
US20070187341A1 (en) * 2005-08-10 2007-08-16 The Regents Of The University Of California Photopolymer serum separator
US20070196433A1 (en) * 2003-04-29 2007-08-23 The Massachusetts General Hospital Corporation Methods and devices for the sustained release of multiple drugs
US20080017577A1 (en) * 2006-07-21 2008-01-24 Becton, Dickinson And Company Membrane-based Double-layer Tube for Sample Collections
US20080132874A1 (en) * 2005-08-10 2008-06-05 The Regents Of The University Of California Collection tubes appratus, systems, and methods
US20090129973A1 (en) * 2005-08-10 2009-05-21 The Regents Of The University Of California Collection Tubes Apparatus, Systems and Methods
US20090139937A1 (en) * 2005-08-10 2009-06-04 The Regents Of The University Of California Polymers for Use in Centrifugal Separation of Liquids
US20100160135A1 (en) * 2008-07-21 2010-06-24 Becton, Dickinson And Company Density Phase Separation Device
US7766900B2 (en) 2005-02-21 2010-08-03 Biomet Manufacturing Corp. Method and apparatus for application of a fluid
US20100288694A1 (en) * 2009-05-15 2010-11-18 Becton, Dickinson And Company Density Phase Separation Device
US20110250105A1 (en) * 2008-11-07 2011-10-13 Kunihiro Suto Blood serum or blood plasma separating material and blood-collecting tube using same
US8182769B2 (en) 2008-04-04 2012-05-22 Biomet Biologics, Llc Clean transportation system
CN102872616A (zh) * 2012-09-26 2013-01-16 成都众睿达科技有限公司 一种血液分离胶及其制备方法
US8377395B2 (en) 2010-04-29 2013-02-19 Charles M. Coleman Integrated blood specimen processor
US8394342B2 (en) 2008-07-21 2013-03-12 Becton, Dickinson And Company Density phase separation device
US8518272B2 (en) 2008-04-04 2013-08-27 Biomet Biologics, Llc Sterile blood separating system
US9333445B2 (en) 2008-07-21 2016-05-10 Becton, Dickinson And Company Density phase separation device
US9669405B2 (en) 2012-10-22 2017-06-06 The Regents Of The University Of California Sterilizable photopolymer serum separator
US9682373B2 (en) 1999-12-03 2017-06-20 Becton, Dickinson And Company Device for separating components of a fluid sample
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid
US20170281466A1 (en) * 2014-09-24 2017-10-05 Q Holding Company Stopper for medicament containers
US11697114B2 (en) * 2015-12-11 2023-07-11 Babson Diagnostics, Inc. Centrifugation method separating serum or plasma from whole blood using a specimen container having a cap to retain blood cells
US12025629B2 (en) 2023-04-14 2024-07-02 Babson Diagnostics, Inc. Automated centrifuge loader

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3508653A (en) * 1967-11-17 1970-04-28 Charles M Coleman Method and apparatus for fluid handling and separation
US3647070A (en) * 1970-06-11 1972-03-07 Technicon Corp Method and apparatus for the provision of fluid interface barriers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3508653A (en) * 1967-11-17 1970-04-28 Charles M Coleman Method and apparatus for fluid handling and separation
US3647070A (en) * 1970-06-11 1972-03-07 Technicon Corp Method and apparatus for the provision of fluid interface barriers

Cited By (153)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3852194A (en) * 1972-12-11 1974-12-03 Corning Glass Works Apparatus and method for fluid collection and partitioning
US3869388A (en) * 1973-06-25 1975-03-04 Continental Oil Co Oil water separation
US4001122A (en) * 1973-08-22 1977-01-04 Telan Corporation Method and device for separating blood components
US4043928A (en) * 1973-10-31 1977-08-23 Lukacs And Jacoby Associates Serum separating composition of matter
JPS51105891A (en) * 1974-01-30 1976-09-20 Wadley Res Inst & Blood Bank Enshinbunryosochi byogenseibiseibutsukenshutsuyosochi oyobi kenshutsuhoho
JPS5812061B2 (ja) * 1974-01-30 1983-03-05 ジエイ ケイ アンド ス−ズイ エル ワドリ リサ−チ インステイテユ−ト アンド ブラド バンク 病原性微生物を濃縮分離するための方法および装置
US3919085A (en) * 1974-02-27 1975-11-11 Becton Dickinson Co Plasma separator assembly
US3957654A (en) * 1974-02-27 1976-05-18 Becton, Dickinson And Company Plasma separator with barrier to eject sealant
US3920557A (en) * 1974-02-27 1975-11-18 Becton Dickinson Co Serum/plasma separator--beads-plus-adhesive type
US3909419A (en) * 1974-02-27 1975-09-30 Becton Dickinson Co Plasma separator with squeezed sealant
US3920549A (en) * 1974-03-18 1975-11-18 Corning Glass Works Method and apparatus for multiphase fluid collection and separation
US3929646A (en) * 1974-07-22 1975-12-30 Technicon Instr Serum separator and fibrin filter
US4189382A (en) * 1974-11-07 1980-02-19 Sherwood Medical Industries Inc. Blood coagulation and separation
FR2295079A1 (fr) * 1974-12-16 1976-07-16 Corning Glass Works Composition stabilisee pour la separation du sang
US4083784A (en) * 1974-12-16 1978-04-11 Corning Glass Works Stabilized blood separating composition
US4049692A (en) * 1974-12-16 1977-09-20 Corning Glass Works Stabilized blood separating composition
US3977982A (en) * 1975-01-20 1976-08-31 Corning Glass Works Controlling viscosity of silica-silicone oil gels
US3957653A (en) * 1975-04-03 1976-05-18 Becton, Dickinson And Company Apparatus for collection, separation and isolation of blood
US3986962A (en) * 1975-07-10 1976-10-19 Becton, Dickinson And Company Novel assembly for separating blood
US3976579A (en) * 1975-07-10 1976-08-24 Becton, Dickinson And Company Novel assembly
US4052320A (en) * 1975-08-29 1977-10-04 Eastman Kodak Company Telescoping serum separator and dispenser
FR2332307A1 (fr) * 1975-11-24 1977-06-17 Gen Electric Compositions de silicone ayant des proprietes rheologiques et une densite determinees
US4071316A (en) * 1975-11-24 1978-01-31 General Electric Company Silicone composition for analyzing blood samples
US4018564A (en) * 1975-11-24 1977-04-19 General Electric Company Silicone composition for analyzing blood samples
US4021340A (en) * 1975-11-28 1977-05-03 Corning Glass Works Blood separating composition
US4055501A (en) * 1976-01-16 1977-10-25 Sherwood Medical Industries Inc. Fluid collection device with phase partitioning means
US4088582A (en) * 1976-01-16 1978-05-09 Sherwood Medical Industries Inc. Blood phase separation means
DE2743882A1 (de) * 1976-09-29 1978-03-30 Nippon Chemiphar Co Trennmittel und verfahren zum auftrennen einer blutprobe in serum- oder plasma- und gerinnsel-anteile
US4152269A (en) * 1977-02-01 1979-05-01 Warner-Lambert Company Collection and separation device
DE2820706A1 (de) * 1977-05-11 1978-11-16 Unilever Emery Copolyesterzusammensetzung
US4350593A (en) * 1977-12-19 1982-09-21 Becton, Dickinson And Company Assembly, compositions and method for separating blood
US4190535A (en) * 1978-02-27 1980-02-26 Corning Glass Works Means for separating lymphocytes and monocytes from anticoagulated blood
US4333564A (en) * 1978-05-22 1982-06-08 Sherwood Medical Industries Inc. Method of controlling rheological properties of gel-like compositions
US4167955A (en) * 1978-06-05 1979-09-18 Beckman Instruments, Inc. Layering a liquid sample onto centrifuge tube liquid contents
US4235725A (en) * 1978-08-16 1980-11-25 Owens-Illinois, Inc. Sterile blood-collecting and separating device
US4279863A (en) * 1979-09-12 1981-07-21 Sherwood Medical Industries, Inc. Reagent separator for a blood collection tube
US4770779A (en) * 1980-05-08 1988-09-13 Terumo Corporation Apparatus for separating blood
US4426290A (en) 1980-05-08 1984-01-17 Terumo Corporation Apparatus for separating blood
US4534798A (en) * 1980-08-18 1985-08-13 Sekisui Kagaku Kogyo Kabushiki Kaisha Composition for partitioning blood components
EP0046391A1 (en) * 1980-08-18 1982-02-24 Sekisui Kagaku Kogyo Kabushiki Kaisha Composition for partitioning blood components
US4386003A (en) * 1981-09-17 1983-05-31 Sherwood Medical Industries Inc. Blood separating composition
US5169543A (en) * 1988-07-26 1992-12-08 Nippon Paint Co., Ltd. Serum separation sealant and fine resin particles using the same
US4994393A (en) * 1989-02-22 1991-02-19 Becton, Dickinson And Company Blood partitioning composition
US5266199A (en) * 1990-11-20 1993-11-30 Nigata Chemicals And Plastics Co., Ltd. Serum separating apparatus
US5269927A (en) * 1991-05-29 1993-12-14 Sherwood Medical Company Separation device for use in blood collection tubes
US5236604A (en) * 1991-05-29 1993-08-17 Sherwood Medical Company Serum separation blood collection tube and the method of using thereof
US5454958A (en) * 1991-05-29 1995-10-03 Sherwood Medical Company Method for sampling in a container having a material therein which separates from a barrier material
US5438000A (en) * 1992-11-12 1995-08-01 Nippon Paint Co., Ltd. Serum separation sealant
US5506333A (en) * 1993-07-30 1996-04-09 Henkel Corporation Polyesters for use in blood partitioning
EP0705882A2 (en) 1994-10-05 1996-04-10 Becton, Dickinson and Company Blood compatible, shear sensitive formulations
US5968018A (en) * 1996-10-30 1999-10-19 Cohesion Corporation Cell separation device and in-line orifice mixer system
US5997811A (en) * 1997-07-02 1999-12-07 Cohesion Technologies, Inc. Method for sterile syringe packaging and handling
US7151000B2 (en) * 1999-03-05 2006-12-19 The Regents Of The University Of California Method of concentrating proteins from serum
US6926834B2 (en) 1999-05-28 2005-08-09 Bio/Data Corporation Method and apparatus for directly sampling a fluid for microfiltration
US6398956B1 (en) 1999-05-28 2002-06-04 Bio/Data Corporation Method and apparatus for directly sampling a fluid for microfiltration
US7288195B2 (en) 1999-05-28 2007-10-30 Bio/Data Corporation Method and apparatus for directly sampling a fluid for microfiltration
US20020084215A1 (en) * 1999-05-28 2002-07-04 Bio/Data Corporation Method and apparatus for directly sampling a fluid for microfiltration
US6740240B2 (en) 1999-05-28 2004-05-25 Bio/Data Corporation Method and apparatus for directly sampling a fluid for microfiltration
US20060060531A1 (en) * 1999-05-28 2006-03-23 Bio/Data Corporation Method and apparatus for directly sampling a fluid for microfiltration
US20040217059A1 (en) * 1999-05-28 2004-11-04 Bio/Data Corporation Method and apparatus for directly sampling a fluid for microfiltration
US7192729B2 (en) 1999-07-06 2007-03-20 General Atomics Methods for assaying homocysteine
US6376210B1 (en) 1999-07-06 2002-04-23 General Atomics Methods and compositions for assaying analytes
US9682373B2 (en) 1999-12-03 2017-06-20 Becton, Dickinson And Company Device for separating components of a fluid sample
US20040014083A1 (en) * 2000-02-25 2004-01-22 Chong-Sheng Yuan Detection of heteroduplex polynucleotides using mutant nucleic acid repair enzymes with attenuated catalytic activity
US6610504B1 (en) 2000-04-10 2003-08-26 General Atomics Methods of determining SAM-dependent methyltransferase activity using a mutant SAH hydrolase
US7070939B2 (en) 2000-10-02 2006-07-04 The Scripps Research Institute Methods for detecting vasculopathies and tumors
US20060211038A1 (en) * 2000-10-02 2006-09-21 Anthony Montgomery Methods for detecting vasculopathies and tumors
US20030215389A1 (en) * 2000-10-02 2003-11-20 Anthony Montgomery Methods for detecting vasculopathies and tumors
EP1552267A2 (en) * 2002-05-17 2005-07-13 Bayer Corporation Serum transfer cup
US6878346B2 (en) 2002-05-17 2005-04-12 Bayer Corporation Serum transfer cup
WO2003099190A3 (en) * 2002-05-17 2004-08-05 Bayer Ag Serum transfer cup
WO2003099190A2 (en) * 2002-05-17 2003-12-04 Bayer Corporation Serum transfer cup
EP1552267A4 (en) * 2002-05-17 2007-06-06 Bayer Corp SERUM TRANSFER CONTAINER
US20030213312A1 (en) * 2002-05-17 2003-11-20 Bayer Corporation Serum transfer cup
US20040072367A1 (en) * 2002-07-26 2004-04-15 Ortho-Clinical Diagnostics, Inc. Metering tip with internal features to control fluid meniscus and oscillation
US7517694B2 (en) * 2002-07-26 2009-04-14 Ortho-Clinical Diagnostics, Inc. Metering tip with internal features to control fluid meniscus and oscillation
US20070196433A1 (en) * 2003-04-29 2007-08-23 The Massachusetts General Hospital Corporation Methods and devices for the sustained release of multiple drugs
US7838024B2 (en) 2003-04-29 2010-11-23 The General Hospital Corporation Methods and devices for the sustained release of multiple drugs
US20090274654A1 (en) * 2003-04-29 2009-11-05 The General Hospital Corporation D/B/A Massachusetts General Hospital Methods and devices for the sustained release of multiple drugs
US7883718B2 (en) 2003-04-29 2011-02-08 The General Hospital Corporation Methods and devices for the sustained release of multiple drugs
US7829112B2 (en) 2003-04-29 2010-11-09 The General Hospital Corporation Methods and devices for the sustained release of multiple drugs
US7833545B2 (en) 2003-04-29 2010-11-16 The General Hospital Corporation Methods and devices for the sustained release of multiple drugs
US20080248017A1 (en) * 2003-04-29 2008-10-09 Massachusetts Institute Of Technology Methods and devices for the sustained release of multiple drugs
US20080286339A1 (en) * 2003-04-29 2008-11-20 The General Hospital Corporation D/B/A Massachusetts General Hospital Methods and devices for the sustained release of multiple drugs
US20080286322A1 (en) * 2003-04-29 2008-11-20 The General Hospital Corporation D/B/A Massachusetts General Hospital Methods and devices for the sustained release of multiple drugs
US20090060982A1 (en) * 2003-04-29 2009-03-05 The General Hospital Corporation D/B/A Massachusetts General Hospital Methods and devices for the sustained release of multiple drugs
US20050244912A1 (en) * 2004-04-30 2005-11-03 Chong-Sheng Yuan Methods for assaying inhibitors of S-adenosylhomocysteine (SAH) hydrolase and S-adenosylmethionine (SAM)-dependent methyltransferase
US7384760B2 (en) 2004-04-30 2008-06-10 General Atomics Methods for assaying inhibitors of S-adenosylhomocysteine (SAH) hydrolase and S-adenosylmethionine (SAM)-dependent methyltransferase
US8444620B2 (en) 2005-02-21 2013-05-21 Biomet Biologics, Llc Method and apparatus for application of a fluid
US9028457B2 (en) 2005-02-21 2015-05-12 Biomet Biologics, Llc Method and apparatus for application of a fluid
US7766900B2 (en) 2005-02-21 2010-08-03 Biomet Manufacturing Corp. Method and apparatus for application of a fluid
US20100314335A1 (en) * 2005-08-10 2010-12-16 The Regents Of The University Of California Photopolymer serum separator
US20090129973A1 (en) * 2005-08-10 2009-05-21 The Regents Of The University Of California Collection Tubes Apparatus, Systems and Methods
US20070187341A1 (en) * 2005-08-10 2007-08-16 The Regents Of The University Of California Photopolymer serum separator
US7673758B2 (en) 2005-08-10 2010-03-09 The Regents Of The University Of California Collection tubes apparatus, systems, and methods
US7674388B2 (en) 2005-08-10 2010-03-09 The Regents Of The University Of California Photopolymer serum separator
US7780861B2 (en) 2005-08-10 2010-08-24 The Regents Of University Of California Photopolymer serum separator
US20090139937A1 (en) * 2005-08-10 2009-06-04 The Regents Of The University Of California Polymers for Use in Centrifugal Separation of Liquids
US8318077B2 (en) * 2005-08-10 2012-11-27 The Regents Of The University Of California Collection tubes apparatus, systems, and methods
US9586203B2 (en) 2005-08-10 2017-03-07 The Regents Of The University Of California Collection tubes apparatus, systems, and methods
US8580183B2 (en) 2005-08-10 2013-11-12 The Regents Of The University Of California Collection tubes apparatus, systems, and methods
US20080132874A1 (en) * 2005-08-10 2008-06-05 The Regents Of The University Of California Collection tubes appratus, systems, and methods
US20100108619A1 (en) * 2005-08-10 2010-05-06 The Regents Of The University Of California Photopolymer serum separator
US7971730B2 (en) 2005-08-10 2011-07-05 The Regents Of The University Of California Collection tubes apparatus, systems and methods
US9248447B2 (en) 2005-08-10 2016-02-02 The Regents Of The University Of California Polymers for use in centrifugal separation of liquids
US8151996B2 (en) 2005-08-10 2012-04-10 The Regents Of The University Of California Photopolymer serum separator
US8936162B2 (en) 2005-08-10 2015-01-20 The Regents Of The University Of California Collection tubes apparatus, systems and methods
WO2007095450A3 (en) * 2006-02-14 2008-02-21 Dade Behring Inc Improved gel compositions, apparatuses and fluid separation methods
US20070190148A1 (en) * 2006-02-14 2007-08-16 Peter Cronin Gel compositions, apparatuses and fluid separation methods
US20080017577A1 (en) * 2006-07-21 2008-01-24 Becton, Dickinson And Company Membrane-based Double-layer Tube for Sample Collections
US8206638B2 (en) 2007-11-01 2012-06-26 The Regents Of The University Of California Collection tubes apparatus, systems, and methods
US20100117269A1 (en) * 2007-11-01 2010-05-13 The Regents Of The University Of California Collection tubes appratus, systems, and methods
US8518272B2 (en) 2008-04-04 2013-08-27 Biomet Biologics, Llc Sterile blood separating system
US8182769B2 (en) 2008-04-04 2012-05-22 Biomet Biologics, Llc Clean transportation system
US9211487B2 (en) 2008-04-04 2015-12-15 Biomet Biologics, Llc Sterile blood separating system
US9700886B2 (en) 2008-07-21 2017-07-11 Becton, Dickinson And Company Density phase separation device
US10350591B2 (en) 2008-07-21 2019-07-16 Becton, Dickinson And Company Density phase separation device
US9714890B2 (en) 2008-07-21 2017-07-25 Becton, Dickinson And Company Density phase separation device
US8747781B2 (en) 2008-07-21 2014-06-10 Becton, Dickinson And Company Density phase separation device
US9933344B2 (en) 2008-07-21 2018-04-03 Becton, Dickinson And Company Density phase separation device
US8394342B2 (en) 2008-07-21 2013-03-12 Becton, Dickinson And Company Density phase separation device
US20100160135A1 (en) * 2008-07-21 2010-06-24 Becton, Dickinson And Company Density Phase Separation Device
US9452427B2 (en) 2008-07-21 2016-09-27 Becton, Dickinson And Company Density phase separation device
US9339741B2 (en) 2008-07-21 2016-05-17 Becton, Dickinson And Company Density phase separation device
US9333445B2 (en) 2008-07-21 2016-05-10 Becton, Dickinson And Company Density phase separation device
US8475742B2 (en) * 2008-11-07 2013-07-02 Hitachi Chemical Co., Ltd. Blood serum or blood plasma separating material and blood-collecting tube using same
US20110250105A1 (en) * 2008-11-07 2011-10-13 Kunihiro Suto Blood serum or blood plasma separating material and blood-collecting tube using same
US9919308B2 (en) 2009-05-15 2018-03-20 Becton, Dickinson And Company Density phase separation device
US9364828B2 (en) 2009-05-15 2016-06-14 Becton, Dickinson And Company Density phase separation device
US20100288694A1 (en) * 2009-05-15 2010-11-18 Becton, Dickinson And Company Density Phase Separation Device
US10456782B2 (en) 2009-05-15 2019-10-29 Becton, Dickinson And Company Density phase separation device
US9919309B2 (en) 2009-05-15 2018-03-20 Becton, Dickinson And Company Density phase separation device
US8794452B2 (en) 2009-05-15 2014-08-05 Becton, Dickinson And Company Density phase separation device
US8998000B2 (en) 2009-05-15 2015-04-07 Becton, Dickinson And Company Density phase separation device
US11786895B2 (en) 2009-05-15 2023-10-17 Becton, Dickinson And Company Density phase separation device
US9731290B2 (en) 2009-05-15 2017-08-15 Becton, Dickinson And Company Density phase separation device
US11351535B2 (en) 2009-05-15 2022-06-07 Becton, Dickinson And Company Density phase separation device
US9802189B2 (en) 2009-05-15 2017-10-31 Becton, Dickinson And Company Density phase separation device
US9919307B2 (en) 2009-05-15 2018-03-20 Becton, Dickinson And Company Density phase separation device
US9079123B2 (en) 2009-05-15 2015-07-14 Becton, Dickinson And Company Density phase separation device
US10807088B2 (en) 2009-05-15 2020-10-20 Becton, Dickinson And Company Density phase separation device
US10413898B2 (en) 2009-05-15 2019-09-17 Becton, Dickinson And Company Density phase separation device
US10343157B2 (en) 2009-05-15 2019-07-09 Becton, Dickinson And Company Density phase separation device
US10376879B2 (en) 2009-05-15 2019-08-13 Becton, Dickinson And Company Density phase separation device
US8377395B2 (en) 2010-04-29 2013-02-19 Charles M. Coleman Integrated blood specimen processor
CN102872616B (zh) * 2012-09-26 2014-10-01 成都众睿达科技有限公司 一种血液分离胶及其制备方法
CN102872616A (zh) * 2012-09-26 2013-01-16 成都众睿达科技有限公司 一种血液分离胶及其制备方法
US9669405B2 (en) 2012-10-22 2017-06-06 The Regents Of The University Of California Sterilizable photopolymer serum separator
US10449117B2 (en) * 2014-09-24 2019-10-22 Q Holding Company Stopper for medicament containers
US20170281466A1 (en) * 2014-09-24 2017-10-05 Q Holding Company Stopper for medicament containers
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid
US11697114B2 (en) * 2015-12-11 2023-07-11 Babson Diagnostics, Inc. Centrifugation method separating serum or plasma from whole blood using a specimen container having a cap to retain blood cells
US12025629B2 (en) 2023-04-14 2024-07-02 Babson Diagnostics, Inc. Automated centrifuge loader

Also Published As

Publication number Publication date
CH611424A5 (ja) 1979-05-31
DE2340199A1 (de) 1975-02-27
GB1443439A (en) 1976-07-21
FR2240668A5 (ja) 1975-03-07
DE2340199B2 (de) 1975-12-11

Similar Documents

Publication Publication Date Title
US3780935A (en) Serum separating method
US5269927A (en) Separation device for use in blood collection tubes
US5560830A (en) Separator float and tubular body for blood collection and separation and method of use thereof
US4088582A (en) Blood phase separation means
JP4306902B2 (ja) 流体サンプルの成分分離用アセンブリおよび方法
US3963119A (en) Serum separating apparatus
US5236604A (en) Serum separation blood collection tube and the method of using thereof
US7578975B2 (en) Device and method for separating components of a fluid sample
CA2011100C (en) Centrifuged material layer measurements taken in an evacuated tube
JP4722284B2 (ja) 流体サンプルの成分分離器具および方法
US4425235A (en) Blood collection device with phase partitioning means
ES2587997T3 (es) Procedimiento de separación de células de una muestra
US4055501A (en) Fluid collection device with phase partitioning means
JP4429521B2 (ja) 流体サンプルの成分分離装置
US3929646A (en) Serum separator and fibrin filter
US4751001A (en) Blood partitioning apparatus
EP1005909B1 (en) Centrifuge tube with round separation element, liner and cap
US3955423A (en) Liquid sampling method
EP0493838A1 (en) Constituent layer harvesting from a centrifuged sample in a tube
US4486315A (en) Immunoassay microparticle washing system and method of use
JPH0225149B2 (ja)
US4707276A (en) Fluid collection device with phase partitioning means
JP2001232243A (ja) 液体試料の成分を分離するための装置および方法
US5251474A (en) Centrifuged material layer measurement in an evacuated tube
US4180465A (en) Fluid collection device with phase separation means

Legal Events

Date Code Title Description
AS Assignment

Owner name: ORGANON TEKNIKA CORPORATION, A CORP. OF DE.

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:LUKACS AND JACOBY ASSOCIATES;REEL/FRAME:005251/0338

Effective date: 19890914