US20220249389A1 - Immunotherapeutic constructs and methods of their use - Google Patents

Immunotherapeutic constructs and methods of their use Download PDF

Info

Publication number
US20220249389A1
US20220249389A1 US17/625,330 US202017625330A US2022249389A1 US 20220249389 A1 US20220249389 A1 US 20220249389A1 US 202017625330 A US202017625330 A US 202017625330A US 2022249389 A1 US2022249389 A1 US 2022249389A1
Authority
US
United States
Prior art keywords
cancer
immunotherapeutic
construct
tumor
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
US17/625,330
Other languages
English (en)
Inventor
Wassana Yantasee
Worapol NGAMCHERDTRAKUL
Amanda LUND
Moataz REDA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oregon Health Science University
PDX Pharmaceuticals Inc
Original Assignee
Oregon Health Science University
PDX Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oregon Health Science University, PDX Pharmaceuticals Inc filed Critical Oregon Health Science University
Priority to US17/625,330 priority Critical patent/US20220249389A1/en
Priority claimed from PCT/US2020/041844 external-priority patent/WO2021011496A1/en
Assigned to PDX PHARMACEUTICALS, INC. reassignment PDX PHARMACEUTICALS, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: NGAMCHERDTRAKUL, Worapol, REDA, MOATAZ
Assigned to OREGON HEALTH & SCIENCE UNIVERSITY reassignment OREGON HEALTH & SCIENCE UNIVERSITY ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: YANTASEE, WASSANA, LUND, AMANDA
Publication of US20220249389A1 publication Critical patent/US20220249389A1/en
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/337—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having four-membered rings, e.g. taxol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/0005—Vertebrate antigens
    • A61K39/0011—Cancer antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
    • A61K39/39541—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against normal tissues, cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
    • A61K39/3955—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against proteinaceous materials, e.g. enzymes, hormones, lymphokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6835—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site
    • A61K47/6851—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment the modifying agent being an antibody or an immunoglobulin bearing at least one antigen-binding site the antibody targeting a determinant of a tumour cell
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6923—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being an inorganic particle, e.g. ceramic particles, silica particles, ferrite or synsorb
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6921—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere
    • A61K47/6927—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores
    • A61K47/6929—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a particulate, a powder, an adsorbate, a bead or a sphere the form being a solid microparticle having no hollow or gas-filled cores the form being a nanoparticle, e.g. an immuno-nanoparticle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0014—Skin, i.e. galenical aspects of topical compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
    • A61K9/167—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction with an outer layer or coating comprising drug; with chemically bound drugs or non-active substances on their surface
    • A61K9/1676—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction with an outer layer or coating comprising drug; with chemically bound drugs or non-active substances on their surface having a drug-free core with discrete complete coating layer containing drug
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/5005—Wall or coating material
    • A61K9/5021—Organic macromolecular compounds
    • A61K9/5031—Organic macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, poly(lactide-co-glycolide)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/51—Nanocapsules; Nanoparticles
    • A61K9/5107—Excipients; Inactive ingredients
    • A61K9/5115—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/51—Nanocapsules; Nanoparticles
    • A61K9/5107—Excipients; Inactive ingredients
    • A61K9/513—Organic macromolecular compounds; Dendrimers
    • A61K9/5146—Organic macromolecular compounds; Dendrimers obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyamines, polyanhydrides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2818—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD28 or CD152
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/515—Animal cells
    • A61K2039/5156—Animal cells expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/515—Animal cells
    • A61K2039/5158—Antigen-pulsed cells, e.g. T-cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55555—Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55561—CpG containing adjuvants; Oligonucleotide containing adjuvants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
    • A61K2039/572—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2 cytotoxic response
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/58—Medicinal preparations containing antigens or antibodies raising an immune response against a target which is not the antigen used for immunisation
    • A61K2039/585—Medicinal preparations containing antigens or antibodies raising an immune response against a target which is not the antigen used for immunisation wherein the target is cancer
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/80—Vaccine for a specifically defined cancer
    • A61K2039/876—Skin, melanoma
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/51—Nanocapsules; Nanoparticles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/17—Immunomodulatory nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/31—Combination therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/32—Special delivery means, e.g. tissue-specific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/30—Special therapeutic applications
    • C12N2320/35—Special therapeutic applications based on a specific dosage / administration regimen

Definitions

  • compositions and methods for treatment and prevention of cancer and other diseases and conditions include immunotherapeutic constructs that include a delivery system (such as a particle) containing at least one therapeutically active agent (which causes tumor antigen release and/or modulates an immunosuppressive tumor microenvironment) and at least one adjuvant (or immunostimulant), creating adaptive immunity by utilizing antigens in subjects' own precancer or cancer cells.
  • a delivery system such as a particle
  • at least one therapeutically active agent which causes tumor antigen release and/or modulates an immunosuppressive tumor microenvironment
  • adjuvant or immunostimulant
  • Immune checkpoint inhibitors such as inhibitors for PD-L1, PD-1, CTLA-4, etc.
  • the treatment works only in a subset of cancer patients ( ⁇ 10-40%).
  • Lack of response is typically due to absence of pre-existing antitumor immunity (e.g., CD8+ T cells) strengthening the need for vaccines to boost the number of anti-tumor T cells in the body.
  • Classical cancer vaccines utilize an immune-stimulator (called adjuvant) and tumor proteins (called antigens). Ideally, neoantigens that exists exclusively on cancer cells should be used. However, these neoantigens vary widely across tumor types and patients, making it difficult and costly to develop personalized vaccines for each patient. To circumvent the need to identify these antigens, radiation, chemotherapy, and engineered viruses such as talimogene laherparepvec (T-VEC) have been used to kill tumors and release the antigens to trigger adaptive immune response in situ.
  • T-VEC talimogene laherparepvec
  • In situ tumor vaccination is a strategy in which tumors are locally killed and release tumor antigens in the presence of immunostimulation, which together prime the systemic adaptive immunity against tumors.
  • TAE tumor microenvironment
  • This strategy has great promise because it circumvents the need to pre-identify tumor (neo)antigens as in conventional cancer vaccine development.
  • This is also a personalized therapy since a unique set of tumor antigens is released and primes specific immunity for each patient.
  • AIRISE Augmenting Immune Response and Inhibiting Suppressive Environment of Tumors.
  • immunotherapeutics generally, immunotherapeutic constructs
  • engineered particles that enable co-delivery of adjuvant(s) and therapeutically active agent(s) that are capable of inducing antigen release (e.g., by killing cancer cells) and/or modulating an immunosuppressive environment (such as a tumor microenvironment, TME).
  • immunotherapeutic constructs may also use antigens already present in the TME.
  • the immunotherapeutic constructs boost the CD8+T cell repertoire and induce systemic anti-tumor immunotherapy effects, without any need to know which antigen(s) are associated with the cancer being treated.
  • humoral immunity antibody generation
  • humoral immunity also plays a role and follows the same concept.
  • therapeutically active agents e.g., siRNA, miRNA, antisense oligonucleotide, mRNA, shRNA, DNA, other oligonucleotides and polynucleotides, small molecule inhibitors, chemotherapeutics, antibodies, etc.
  • delivered to cancer cells by the provided engineered immunotherapeutic constructs will kill cancer cells, releasing tumor antigens, and/or manipulate the immunosuppressive tumor microenvironment, while the co-delivered adjuvants (e.g., CpG, R848, poly I:C, etc.) prime and activate adaptive immune cells against the tumor antigens.
  • co-delivered adjuvants e.g., CpG, R848, poly I:C, etc.
  • the activated effector cells can recognize and attack tumors at any sites in the body (including sites remote from localized delivery of the immunotherapeutic constructs) as well as reducing or even preventing the spread or the development of new tumors harboring one or more of the same tumor antigens as the treated tumors. This phenomenon is sometimes referred to as the abscopal effect. Death of cancer cells further amplifies the adaptive immunity loop with long-lasting effects. Memory adaptive immunity will also be established for continuous antitumor immune surveillance.
  • the immunotherapeutic constructs can be administered locally, intratumorally, intranasally, intraperitoneally, intracerobrospinally, subcutaneously, intra-articularly, intrasynovially, intrathecally, orally, topically, dermally, intravenously, or by inhalation, e.g., to readily accessible tumors such as melanoma, head and neck cancer, breast cancer, colon cancer, ovarian cancer, bladder cancer, and lymphoma; or systemically for other cancers such as lung cancer, liver cancer, pancreatic cancer, prostate cancer, brain cancer, kidney cancer, blood cancer, and metastatic cancers.
  • Engineered immunotherapeutic constructs can have a diameter in the nanometers or micrometer range, and can be made of any materials (e.g., lipid, inorganic materials, polymers, and their combinations) capable of loading the therapeutic agents/adjuvant cargos, delivering them to the target sites (cancer cells, immune cells, extracellular matrices, etc.), and allowing them to have the desired functions.
  • materials e.g., lipid, inorganic materials, polymers, and their combinations
  • immunotherapeutic constructs also contain one or more homing agents (antibodies, aptamers, ligands, peptides, etc.) that enable them to be preferentially delivered to and/or taken up by target cancer cells and/or various immune cell types (e.g., dendritic cells (DCs), macrophages, monocytes, T cells).
  • homing agents antibodies, aptamers, ligands, peptides, etc.
  • DCs dendritic cells
  • macrophages e.g., monocytes, T cells.
  • immunotherapeutic constructs may be used alone or in combination with standard therapeutics, including, but not limited to, immune checkpoint inhibitors, chemotherapy, surgery, targeted therapies, and radiation therapy.
  • checkpoint inhibitors siRNA, inhibitors, or antibody against for PD-L1/PD-1, CTLA-4, etc.
  • other targeted therapeutics e.g., small molecule inhibitors or antibodies targeting other oncoproteins, or medical radioactive isotopes
  • the immunotherapeutic constructs optionally can be formulated into topical or microneedle formulations.
  • an immunotherapeutic construct including: a delivery system including at least one therapeutic agent that causes tumor antigen release and/or modulates an immunosuppressive tumor microenvironment; and at least one adjuvant.
  • the immunotherapeutic construct may not include a tumor-specific antigen or ovalbumin.
  • the immunotherapeutic construct does not include a protein other than the one therapeutic agent or the at least one adjuvant, if either is a protein.
  • the therapeutic agent and adjuvant may be loaded into, attached to the surface of, coupled to, enclosed within, or contained within the delivery system.
  • the delivery system are nanoparticles with a hydrodynamic size of 5 nm to 999 nm (e.g., about 80 nm to about 200 nm, or about 90 nm to about 130 nm), as measured in an aqueous medium (such as PBS, Tris-buffer, or water).
  • an aqueous medium such as PBS, Tris-buffer, or water.
  • the immunotherapeutic constructs are microparticles with a hydrodynamic size of 1 micron to 1000 micron.
  • the delivery system has a size of about 5 nm to about 200 nm, about 5 nm to about 90 nm, about 5 nm to about 20 nm, about 30 nm to about 100 nm, about 30 nm to about 80 nm, about 30 nm to about 60 nm, about 40 nm to about 80 nm, about 70 nm to about 90 nm, or about 5 nm, about 10 nm, about 20 nm, about 30 nm, about 40 nm, about 50 nm, about 60 nm, about 70 nm, about 80 nm, about 90 nm, or about 100 nm.
  • the therapeutic agent includes an oligonucleotide (e.g., a siRNA, a miRNA, an antisense oligonucleotide, a mRNA, a DNA, a shRNA, or a sgRNA (CRISPR-cas9 element)), a polynucleotide, a peptide, a protein, a chemotherapy drug, a toxin, an antioxidant, a small molecule inhibitor, an antibody, or a radio-therapeutic agent.
  • an oligonucleotide e.g., a siRNA, a miRNA, an antisense oligonucleotide, a mRNA, a DNA, a shRNA, or a sgRNA (CRISPR-cas9 element)
  • a polynucleotide e.g., a siRNA, a miRNA, an antisense oligonucleotide, a mRNA, a DNA, a shRNA, or
  • the adjuvant compound has immunostimulatory activity.
  • adjuvant compounds may include one or more of a TLR-binding DNA substituent, such as a CpG oligonucleotide (e.g., ISS 1018; Amplivax; CpG ODN 7909, CpG ODN 1826, CpG ODN D19, CpG ODN 1585, CpG ODN 2216, CpG ODN 2336, ODN 1668, ODN 1826, ODN 2006, ODN 2007, ODN 2395, ODN M362, or SD-101); a DNA TLR agonist that contains CpG sequences (e.g., dSLIM); a non-CpG DNA TLR agonist (e.g., EnanDIM); a RNA TLR agonist (e.g., Poly I:C or Poly-ICLC); an aluminum salt (e.g., aluminum hydroxide, aluminum phosphate, aluminum chloride, or
  • an immunotherapeutic construct can be used as a prophylactic vaccine in patients (including and especially those that are genetically predisposed) with a large number of atypical nevi or other seemingly benign moles.
  • the immunotherapeutic construct can be given to accessible tumors/lesions via intratumoral/intralesional injection prior to surgical removal (i.e. neoadjuvant setting) to lessen the chance of recurrence and/or recruit immune system to kill any detectable or undetectable metastases.
  • the immunotherapeutic construct can be given to a tumor via intratumoral injection even though the tumor is unresectable. This will activate and recruit the immune system to attack both the treated tumor and untreated tumors elsewhere in the body.
  • the immunotherapeutic construct can be given systemically to initiate anti-tumor adaptive immune response.
  • the immunotherapeutic construct may be given to the area around the tumor (peritumoral) or the area remaining after tumor removal (adjuvant setting).
  • the immunotherapeutic construct can be given systemically, which will develop adaptive immunity against cancer anywhere in the body.
  • the immunotherapeutic construct may be administered directly into lymph nodes (with or without detectable tumors).
  • Another embodiment is a method of treating a cell obtained from a subject exhibiting symptoms of cancer, which method includes contacting the cell with a therapeutically effective amount of an immunotherapeutic construct of any one of the described embodiments, or a composition including an immunotherapeutic construct.
  • the cell obtained from the subject is a cancer cell.
  • the cell is not a cancer cell; for instance, in some instances the non-cancer (e.g., normal) cell is an immunology/immune cell.
  • the method further includes administering at least one treated cell back to the subject.
  • Administering an immunotherapeutic construct in any of the described method embodiments may include one or more of: injection directly into a tumor in the subject; systemic injection in the subject; topical application to the subject; inhalation by the subject; hepatic arterial infusion to the subject; convection-enhanced delivery to the subject; or microneedle application to the subject.
  • the subject (being treated, to which a construct or composition is being administered, or from which a cell is obtained) is a mammal; for instance, in certain embodiments the mammal is a human.
  • any of the provided immunotherapeutic construct embodiments, and embodiments of methods of using such constructs include examples in which the immunotherapeutic construct does not include a tumor-specific antigen or ovalbumin.
  • FIG. 1 Cancer treatment.
  • Targeted therapy has improved cancer prognosis significantly over non-specific toxic chemotherapy.
  • the effect is not sustained.
  • Immune checkpoint inhibitors (ICIs) unleash the body's own immune system to attack cancer, potentially resulting in cure.
  • AIRISE Treatment Immune checkpoint inhibitors
  • Our goal is to develop a novel immunotherapeutic construct (Augmenting Immune Response and Inhibiting Suppressive Environment of tumors—AIRISE) that manipulate the tumor microenvironment (TME) to boost anti-tumor T cell repertoires, which will increase the cure rate of cancer patients treated with ICIs.
  • AIRISE Treatment Immune checkpoint inhibitors
  • TEE tumor microenvironment
  • FIG. 2 In situ tumor vaccination mechanism of our novel immunotherapeutic constructs.
  • Only one of the tumors e.g., a melanoma or breast tumor
  • AIRISE a melanoma or breast tumor
  • DTX docetaxel
  • APCs local antigen-presenting cells
  • DCs dendritic
  • siSTAT3 can kill some cancer cells, while knocking down STAT3 reduces immunosuppressive tumor microenvironment that prevents the priming, activation, and function of anti-tumor adaptive immune response.
  • Tumor antigens already in the tumor microenvironment (TME, including cancer, immune cells, etc.), or released by our treatment will be taken up by the AIRISE-activated APCs in the tumor and tumor-draining lymph node(s). APCs then (cross) present these antigens to prime tumor antigen-specific T cells. These activated cytotoxic (effector CD8+) T cells will proliferate and enter systemic circulation.
  • FIG. 3 Greater activation of dendritic cells (MHCII+ CD80+ CD11c+ cells) after treatment with CpG-NP over CpG.
  • CpG or CpG-NP was administered to mice by footpad injection.
  • One day after treatment draining (DLN) and non-draining lymph nodes (NDLN) were collected and processed into single cells for flow cytometry analysis to identify % activated dendritic cells.
  • the CpG ODN 1826 (SEQ ID NO: 7) was used throughout the examples unless otherwise specified.
  • FIGS. 4A-4D Effectiveness of CpG-NP administered to a melanoma mouse model as in FIG. 4A (mouse implanted with bilateral tumors) in inducing in situ tumor vaccination as indicated by inhibited tumor growth curves of local treated tumors ( FIG. 4B ) and distant untreated tumors ( FIG. 4C ) and prolonged survival curve of the mice ( FIG. 4D ). 250,000 and 100,000 B16F10 cells were implanted into each mouse (C57BL/6) to establish local and distant tumors, respectively. Eight days after tumor implantation, CpG-NP or saline was intratumorally injected into the local tumor every three days for a total of three doses.
  • FIGS. 5A-5D Effectiveness of NP loaded with CpG and/or docetaxel (DTX) (AIRISE-01) administered to a melanoma mouse model as in FIG. 5A in inducing in situ tumor vaccination as indicated by inhibited tumor growth curves of local treated tumors ( FIG. 5B ) and distant untreated tumors ( FIG. 5C ), and prolonged survival curve of the mice ( FIG. 5D ).
  • Mice (same model as FIG. 4 ) were treated with CpG-NP, CpG-DTX-NP or saline.
  • FIG. 6 Increase in cytotoxic CD8+ T cells in the local and distant tumors, DLN of local (treated) and distant tumors, and non-DLN triggered by CpG-DTX-NP (denoted as NP in this figure). All conditions are the same with FIG. 5A . Tumors and lymph nodes (tumor-draining and non-draining) were collected 7 days after the first injection (see FIG. 5A ). Cells were stained with a panel of antibodies to evaluate the lymphoid cell population and activity. p-values: *p ⁇ 0.05. **p ⁇ 0.01, ***p ⁇ 0.001.
  • FIGS. 7A-7D Effectiveness of siSTAT3-CpG-NP (AIRISE-02) administered to a melanoma mouse model as in FIG. 7A , in inducing in situ tumor vaccination as indicated by inhibited tumor growth curves of local treated tumors ( FIG. 7B ) and distant untreated tumors ( FIG. 7C ), and prolonged survival curve of the mice ( FIG. 7D ). Tumor volumes are plotted as mean and SEM. Dose (per each injection): 20 ⁇ g CpG; 4 ⁇ g siSTAT3; 0.2 mg NP. Statistical difference (p-values as specified, *) was evaluated between CpG-NP and siSTAT3-CpG-NP (two groups that respond the best).
  • FIGS. 8A-8D The CD8-dependency of siSTAT3-CpG-NP (AIRISE-02)'s effect.
  • C57/BL6 mice bearing B16F10 tumors were established and treated as in FIG. 8A , and CD8 depleting antibodies (Clone 2.43, BioXcell, 200 ⁇ g/mouse, twice weekly, i.p.) were given to a group of mice throughout the entire study, starting 1 day before the first intratumoral treatment of AIRISE-02.
  • CD8 depletion was shown to lessen the efficacy of AIRISE-02 in inhibiting local tumors ( FIG. 8B ), distant untreated tumors ( FIG. 8C ), and prolonging the survival of mice ( FIG. 8D ), indicating that AIRISE-02's effect is immune-dependent rather than directly cytotoxic.
  • FIGS. 9A-9D siSTAT3-CpG-NP (AIRISE-02) enhanced the effects of checkpoint inhibitors (PD1 and CTLA4 antibodies).
  • C57/BL6 mice bearing B16F10 tumors were established and treated as in ( FIG. 9A ).
  • Checkpoint inhibitors 200 ⁇ g PD1 mAb/mouse and 100 ⁇ g CTLA4 mAb/mouse, i.p.
  • AIRISE-02 greatly enhanced the effects of checkpoint inhibitor cocktail; the combination controlled both local ( FIG. 9B ) and distant untreated tumors ( FIG. 9C ), and prolonged survival of mice ( FIG. 9D ) better than AIRISE-02 or checkpoint inhibitor cocktail alone. 5 out of 8 mice receiving the combination were cured (tumor-free).
  • FIGS. 10A-10B siSTAT3-CpG-NP (AIRISE-02) prolonged survival of mice bearing experimental metastatic lung tumors.
  • C57/BL6 mice was injected (via tail vein) with 200,000 Lewis Lung Carcinoma (LLC-JSP) cells resulting in lung cancer establishment in lung of the mice.
  • Treatments were given intravenously as shown in FIG. 10A .
  • Survival was significantly prolonged with intravenous AIRISE-02 as shown in FIG. 10B .
  • FIGS. 11A-11D CpG and siSTAT3 delivered with cationic lipid particles (DharmaFECT) yields in situ vaccination effect.
  • C57/BL6 mice bearing B16F10 tumors were established and treated as in FIG. 11A .
  • the therapeutic construct reduced treated tumors ( FIG. 11B ) and distant tumors ( FIG. 11C ), as well as prolonged survival of mice ( FIG. 11D ).
  • the therapeutic construct has the average size of 1068 nm (1.1 micron) as measured by DLS.
  • FIGS. 12A-12C NP-mediated co-delivery of siRNAs and CpG to cancer and immune cells.
  • B16F10 ( FIG. 12A ) and J774 ( FIG. 12B ) cells both were mouse cell lines) and ( FIG. 12C ) dendritic cells harvested from bone marrow of C3H/HEJ mice (BMDC) were treated with NP or CpG loaded NP (CpG-NP) carrying siRNA against STAT3 or scrambled siRNA (siSCR).
  • Dose of each siRNA was 50 nM and at 2.0 wt. % of NP, and dose of CpG was 2 wt.
  • NP denotes mesoporous silica nanoparticles coated with cross-linked PEI and PEG as described in Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015 and U.S. Patent Application Publication No. 2017/0173169.
  • FIG. 13 HCC1954 cells (human HER2+ cancer cells) were treated with trastuzumab-conjugated NP (T-NP) carrying siRNA against HER2 or STAT3. Dose of each siRNA was 30 nM and at 2.0 wt. % of NP throughout. Proteins were analyzed with Western Blot at 72 h post-treatment and indicated 80% knockdown of STAT3 was achieved. Data indicate that the nanoparticles can deliver at least two siRNA sequences (e.g., siHER2 and siSTAT3) without losing the efficacy compared to a single siRNA.
  • T-NP trastuzumab-conjugated NP
  • FIGS. 14A-14C Preferential uptake of nanoparticle containing antibody as homing target agent. After 1 hour of exposure, EGFR antibody (cetuximab) conjugated nanoparticles (C-NP) were preferentially taken up by lung cancer cells overexpressing EGFR (A549 and H460) over normal lung cells (NL20) as shown in FIG. 14A .
  • FIG. 14B shows the level of EGFR expression of these cell lines as measured by a flow cytometry. Likewise, FIG.
  • T-siSCR-NP HER2 antibody conjugated nanoparticles
  • HER2 antibody conjugated nanoparticles T-siSCR-NP
  • T-siSCR-NP HER2 antibody conjugated nanoparticles
  • BT474, SKBR3 breast cancer cells overexpressing HER2 (BT474, SKBR3) over MCF7 (with low HER2 expression, as shown by Western Blot analysis (Inset of ( FIG. 14C )).
  • the preferential effect was not observed with rituximab (CD20 antibody) conjugated nanoparticles (R-siSCR-T).
  • siSCR denoted scrambled siRNA.
  • FIG. 15 Adding CpG (SEQ ID NO: 7) to nanoparticles containing PLK1 inhibitor (p-iPLK1-NP) enhances therapeutic benefit as demonstrated by Kaplan Meier Survival curve.
  • 100K LLC-JSP cells lung cancer cells
  • 40K cells were injected in left flank of C57BL/6 mice.
  • mice received intratumoral treatments of saline, PD-L1 antibody coated nanoparticle (p-NP), nanoparticle loaded with PLK1 inhibitor (iPLK1-NP), p-NP loaded with PLK1 inhibitor (p-iPLK1-NP), or p-NP loaded with PLK1 inhibitor and CpG (p-iPLK1-NP-CpG) to the right (local) tumor.
  • p-NP PD-L1 antibody coated nanoparticle
  • iPLK1-NP nanoparticle loaded with PLK1 inhibitor
  • p-iPLK1-NP p-NP loaded with PLK1 inhibitor
  • CpG p-iPLK1-NP-CpG
  • FIGS. 16A-D Effectiveness of NP loaded with CpG and mitoxantrone (MTX) administered to a melanoma mouse model as in FIG. 16A , in inducing in situ tumor vaccination as indicated by inhibited tumor growth curves of local treated tumors ( FIG. 16B ) and distant untreated tumors ( FIG. 16C ), and prolonged survival curve of the mice ( FIG. 16D ).
  • Mice were treated with CpG-MTX-NP or saline. Dose (per each injection): 20 ⁇ g CpG; 2 ⁇ g MTX; 0.2 mg NP. Tumor volumes are plotted as mean and SEM. For tumor volume, **p ⁇ 0.01 for CpG-MTX-NP vs. Saline.
  • FIGS. 17A-C AIRISE-02 enhanced proliferation of CD8+ T cells in local (treated) and untreated tumors and their tumor draining lymph nodes (DLN).
  • FIG. 18 NP cell uptake in the TME.
  • FIG. 19 siSTAT3-NP can knock down STAT3 in multiple cells of multiple species.
  • D-17 dog osteosarcoma
  • BMDC Breast Cell-derived dendritic cells from mice
  • J774 mouse macrophage
  • B16F10 mouse melanoma
  • HCC1954 human breast cancer
  • siSTAT3-NP 50 nM
  • qRT-PCR analysis for STAT3 and HPRT mRNA was performed with primers of corresponding species.
  • a single siSTAT3 sequence was used throughout.
  • siSCR scrambled siRNA control. ***p ⁇ 0.001; ****p ⁇ 0.0001.
  • FIG. 20 AIRISE-02 (siSTAT3-CpG-NP)+ICIs resulted in complete response in mice bearing CT26 bilateral tumors. 250K and 100K CT26 cells were implanted into bilateral abdomens of each mouse (Balb/c). 15 days after tumor implantation, mice were treated as outlined. Tumor growth curves of local treated tumors and distant untreated tumors are plotted as spider plots (each line represents an individual mouse). Dose of injection: 16 ⁇ g CpG; 5 ⁇ g siSTAT3; 0.25 mg NP.
  • Checkpoint inhibitors 200 ⁇ g PD1 mAb/mouse and 100 ⁇ g CTLA4 mAb/mouse, i.p. were given to two groups of mice concurrently with the intratumoral AIRISE-02 in one group and alone in one group.
  • FIG. 21 AIRISE-02 (siSTAT3-CpG-NP)+ICIs are also effective in mice bearing aggressive 4T1 breast bilateral tumors. 100K and 40K 4T1 cells were implanted into bilateral mammary fat pads of each mouse (Balb/c). 11 days after tumor implantation, mice were treated as outlined. Tumor growth curves of local treated tumors and distant untreated tumors are plotted as spider plots (each line represents individual mouse). Dose is the same as FIG. 20 .
  • FIG. 22 Survival curve of mice in FIG. 21 .
  • FIG. 23 Safety profile of siSTAT3-CpG-NP (AIRISE-02).
  • AIRISE-02 was administered intramuscularly to three female Balb/c mice. Mice were depilated and injected once in the caudal thigh muscle. Images of the injection site were taken pre-injection, post-injection, 24 hours post-injection, and 72-post injection. Dose of injection: 16 ⁇ g CpG; 5 ⁇ g siSTAT3; 0.25 mg NP.
  • FIGS. 24A-B Safety profile of AIRISE-02 in mice.
  • Mice (Balb/c) bearing bilateral MM3MG-HER2d16 tumors implanted in mammary fat pads (as described in Tsao et al., JCI Insight, 4(24):e131882, 2019) were treated with AIRISE-02 by intratumoral injection into one of the two tumors in each mouse 5 times over two weeks. Body weight is monitored as shown in (A); Mice were euthanized when the tumor exceeded 2 cm in diameter or when the mice exhibited signs of pain or distress (15-55 days after treatment). Upon euthanasia, blood were collected and processed into serum. Serum biomarker was measured by Beckman AU680 (IDEXX BioAnalytics, West Sacramento, Calif.) and reported in (B). Dose is the same as FIG. 20 .
  • FIG. 25 Schematic describing safety profile of AIRISE-02 in Cynomolgus monkey.
  • CpG 7909/2006 human sequence was used (SEQ ID NO: 8).
  • Body weight, food consumption, cage-side and detailed observations, mortality, morbidity, reaction at the injection site, PK, clinical pathology, cytokine levels, complement split products, and anti-drug antibody were monitored.
  • FIG. 26 Hydrodynamic size of mesoporous silica nanoparticles coated with cross-linked PEI and PEG (NP) loaded with different amount of siRNA and CpG, specified as wt % of the whole construct. Average size (Z-average) and polydispersity index (PDI) is shown from 3 measurements using Malvern Zetasizer.
  • FIG. 27 CpG-NP can generate antigen-specific (adaptive) immune response in the presence of antigen.
  • Figure shows the percentage of IFN ⁇ activated CD8+ T cells after incubation in the presence of SF (SIINFEKL peptide).
  • the cells were obtained from lymph nodes of untreated mice, mice treated with NPs loaded with SF and CpG (CpG-SF-NP), NPs loaded with SF (SF-NP), mice or NP loaded with CpG (CpG-NP), and mice treated with SF formulated with Incomplete Freund's Adjuvant (IFA/SF). *p ⁇ 0.05.
  • FIG. 28 Hydrodynamic size of nanoparticles (MSNP-PEI-PEG) loaded with poly I:C at about 2 wt. % and 9 wt. %, measured in PBS.
  • FIGS. 29A-C AIRISE-02.
  • A TEM image of mesoporous silica nanoparticle core
  • B Schematic of AIRISE-02 comprising a mesoporous silica nanoparticle coated with PEI, which is cross-linked as described prior (Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015), conjugated with PEG, resulting in a nanoparticle construct (NP).
  • NP nanoparticle construct
  • NP was loaded with siSTAT3 and CpG by electrostatic interaction by 10-40 min mixing in PBS.
  • C Hydrodynamic size of AIRISE-02 ((2%)siSTAT3-(6%)CpG-NP).
  • FIG. 30 Topical siRNA-NP in pig skin with and without microneedle roller pre-treatment. Fluorescent images of pig skin treated with one topical application of Dy677-siSCR-NP in Aquaphor for one hour with and without pre-treating skin with a microneedle roller. siRNA signal is noted with arrows. Tissues were also stained for nuclei with Hoechst 33342.
  • FIG. 31 Topical siRNA-NP/Tween-Aquaphor in mice with and without microneedle roller pre-treatment. Fluorescent images of mouse skin treated with one topical application of Dy677-siSCR-NP in Tween/Aquaphor for 1.5 hour with and without pre-treating skin with a microneedle roller. siRNA signal is noted with arrows. Tissues were also stained for nuclei with Hoechst 33342.
  • FIGS. 32A-B EGFR knock down efficacy of topical siRNA-NP with microneedle roller versus injected siRNA-NP.
  • Mouse skin was harvested at 3 days after one topical treatment with siEGFR-NP or siSCR-NP in Tween/Aquaphor with microneedle roller application (A) or 3 days after one injection of siEGFR-NP or siSCR-NP in saline (B). Skin tissue was fixed and stained with fluorescently labelled EGFR antibody for EGFR signal quantification. 4-8 images (20 ⁇ ) were processed per condition and 3 animals per group.
  • FIG. 33 Dextran-based microneedle containing NP loaded with Dy677-siRNA.
  • FIGS. 34A-B Viability of mouse (A) bone marrow derived dendritic cells and (B) J774 cells, 2 days after treatment with AIRISE-02 containing different amount of CpG and 2 wt % siSTAT3. Dose: 50 nM siRNA.
  • FIG. 35 Co-delivery of non-targeting scrambled siRNA (siSCR) and CpG by NP or Dharmafect to dendritic cells harvested from C3H/HEJ mice. Dose of each siRNA was 50 nM and at 2.0 wt. % of NP, and dose of CpG was 4 wt. % of NP. siRNA-Dharmafect formulation was prepared following the manufacturer's protocol. mRNA was analyzed with qRT-PCR at 48 h post-treatment.
  • siRNA-Dharmafect formulation was prepared following the manufacturer's protocol.
  • NP denotes mesoporous silica nanoparticles coated with cross-linked PEI and PEG as described in Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015 and U.S. Patent Application Publication No. 2017/0173169.
  • FIG. 36 siSTAT3/siCXCR4-CpG-NP+ICIs are effective in mice bearing aggressive 4T1 breast tumors.
  • 100K and 40K 4T1 cells were implanted into bilateral mammary fat pads of each mouse (Balb/c). 8 days after tumor implantation, mice were treated with ICIs with or without siSTAT3/siCXCR4-CpG-NP in a similar manner to FIGS. 20 and 21 ( 3 doses; 3 days apart).
  • Tumor growth curves of local treated tumors and distant untreated tumors are plotted as spider plots (each line represents individual mouse).
  • Dose of injection 16 ⁇ g CpG; 5 ⁇ g siSTAT3; 5 ⁇ g siCXCR4; 0.25 mg NP.
  • nucleic acid sequences described herein are shown using standard letter abbreviations for nucleotide bases, as defined in 37 C.F.R. ⁇ 1.822. Only one strand of each nucleic acid sequence is shown, but the complementary strand is understood as included in embodiments where it would be appropriate.
  • a computer readable text file, entitled “2L05917.txt” created on or about Nov. 30, 2021, with a file size of 4 KB, contains the sequence listing for this application and is hereby incorporated by reference in its entirety.
  • SEQ ID NO: 1 is a representative sense sequence of an siRNA specific for STAT3 (siSTAT3): 5′ GGAUCUAGAACAGAAAAUGdTdT 3′ (the last two positions of which are deoxy bases).
  • SEQ ID NO: 2 is a representative antisense sequence of an siRNA specific for STAT3 (siSTAT3): 5′ CAUUUUCUGUUCUAGAUCCdTdG 3′ (the last two positions of which are deoxy bases).
  • SEQ ID NO: 3 is a representative sense sequence of an siRNA specific for HER2 (siHER2): 5′ CACGUUUGAGUCCAUGCCCAAUU 3′.
  • SEQ ID NO: 4 is a representative antisense sequence of an siRNA specific for HER2 (siHER2): 5′ UUGGGCAUGGACUCAAACGUGUU 3′.
  • SEQ ID NO: 5 is a representative sense sequence of an siRNA specific for SCR (siSCR): 5′ UGGUUUACAUGCGACUAA 3′.
  • SEQ ID NO: 6 is a representative antisense sequence of an siRNA specific for SCR (siSCR): 5′ UUACUCGACAUGUAAACCA 3′.
  • SEQ ID NO: 7 is the sequence of CpG ODN 1826, which was used throughout the examples (mouse system): 5′-TCCATGACGTTCCTGACGTT-3′. This ODN contains a full phosphorothioate backbone and is nuclease resistant.
  • SEQ ID NO: 8 is the sequence of CpG ODN 2006/7909, which was used in examples with monkey and human system: 5′-TCGTCGTTTTGTCGTTTTGTCGTT-3′. This ODN contains a full phosphorothioate backbone and is nuclease resistant.
  • the herein described immunotherapeutic approach called AIRISE (Augmenting Immune Response and Inhibiting Suppressive Environment of Tumors) for cancer treatment utilizes patients' own tumors as a depot for a personalized set of tumor antigens (in situ tumor vaccination).
  • the provided immunotherapeutic constructs carry at least one adjuvant (e.g., a CpG oligonucleotide) and one or more therapeutic agents/compounds (e.g., a siRNA, an antisense, oligonucleotide, a drug, a small molecule, an antibody, etc.) that cause antigen release and/or modulate immunosuppressive tumor microenvironment.
  • therapeutic agents are docetaxel and siRNA against STAT3.
  • tumor antigen(s) are released in the presence of immunostimulation (provided by the supplied adjuvant(s)). This antigen release and immunostimulation together initiate and support antigen-specific adaptive immunity.
  • Tumor antigens can be taken up by existing antigen-presenting cells (APCs), which present the antigen to na ⁇ ve T cells.
  • APCs antigen-presenting cells
  • T cells are thereby primed and activated into effector T cells (either in lymph nodes or in tumor site) and proliferate throughout the body, thus providing increased and improved immune response to the treated tumor as well as to other tumors remote from the initial administration (e.g., metastatic sites).
  • effector T cells either in lymph nodes or in tumor site
  • the efficacy in tumors remote from the initial administration site is also known in the field as an abscopal effect.
  • the cargo combination can be applied on any type of micro/nanoparticles—that is, embodiments of the immunotherapeutic constructs and methods of their use are delivery vehicle agnostic. Specific example delivery vehicles are described herein.
  • the invention provides in situ tumor vaccination effect(s) using the herein-described immunotherapy, exemplified with immunotherapeutic constructs loaded with CpG and siSTAT3 (siSTAT3-CpG-NP), or with CpG and docetaxel (CpG/DTX-NP).
  • immunotherapeutic constructs loaded with CpG and siSTAT3 (siSTAT3-CpG-NP), or with CpG and docetaxel (CpG/DTX-NP).
  • DTX docetaxel
  • DTX will kill local cancer cells to release tumor antigens
  • CpG will activate local antigen-presenting cells (APCs, primarily DCs)
  • siSTAT3 will kill some cancer cells, its main role is to reduce immunosuppressive tumor microenvironment (TME) that prevents priming and action of anti-tumor adaptive immune response.
  • TEE immunosuppressive tumor microenvironment
  • siSTAT3-CpG-NP is designed to be taken up by both cancer and APCs. While it may have some killing effect in some cancer cells, it will not kill, but rather activate APCs by knocking down STAT3.
  • Tumor antigens (already in the TME, or released by the provided treatment) are taken up by CpG-activated or AIRISE-activated APCs in the tumor and tumor-draining lymph node. APCs then (cross) present these antigens to prime tumor antigen-specific T cells. These activated cytotoxic (effector) T cells will proliferate and enter systemic circulation.
  • antioxidant mesoporous silica nanoparticle can modulate local immunosuppressive TME further, inhibiting pro-tumoral activities. This vaccination induced locally at the tumor site generates whole-body systemic anti-tumor immunity.
  • the delivery vehicle includes a MSNP core (e.g., ⁇ 50 nm) for drug loading, coated with a bioreducible cross-linked cationic polymer, e.g., polyethyleneimine (PEI), for oligo loading and endosomal escape; and a stabilizer, e.g., polyethylene glycol (PEG), which prevents nanoparticle aggregation, protects oligo cargos from degradation by blood enzymes (Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015), and shields the charge of PEI, enhancing safety.
  • a bioreducible cross-linked cationic polymer e.g., polyethyleneimine (PEI)
  • PEI polyethyleneimine
  • PEG polyethylene glycol
  • Oligo is loaded last on the construct with a few minutes (e.g., 5 minutes) mixing in PBS at room temperature; it electrostatically binds to PEI in an oligo sequence-independent manner and is protected under the PEG layer from enzymatic degradation (Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015).
  • the resulting nanoparticle (NP) was highly optimized for siRNA delivery efficacy in terms of MSNP sizes, PEI and PEG molecular weights and compositions, PEI crosslinking conditions (to enhance buffering capacity and lower charge), oligo and (optionally) antibody loadings (Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015).
  • This embodiment of the siRNA-NP has a rigid MSNP core size (by TEM) of 50 nm and hydrodynamic size (NP with polymer coatings) of 100 nm with a narrow size distribution. It includes 13.5 wt. % PEI, 18.2 wt. % PEG, and can load 2-4 wt. % siRNA or up to 10 wt.
  • Drug e.g., taxane
  • MSNP core or on the polymers can be loaded in the MSNP core or on the polymers at 0.5-3 wt. %. All values in this paragraph are by weight of the nanoconstruct. See also U.S. Patent Application Publication No. 2017/0172923.
  • an immunotherapeutic construct including: a delivery system; at least one therapeutic agent, e.g., loaded into, attached to the surface of, coupled to, enclosed within, or contained within the delivery system, where therapeutic agent causes tumor antigen release and/or modulates an immunosuppressive tumor microenvironment; and at least one adjuvant compound, e.g., attached to the surface of, coupled to, enclosed within, or contained within the delivery system is provided.
  • the delivery system includes (or, in other embodiments, is) a liposome, a lipid-based particle, a polymeric particle, an inorganic particle, an inorganic particle coated with polymer or lipid, or a hybrid thereof.
  • the delivery vehicle is an inorganic particle and includes one or more of mesoporous silica, gold, aluminum, calcium phosphate, iron oxide, or an antioxidant particle (such as cerium oxide).
  • the delivery vehicle includes one or more of fullerenes, endohedral metallofullerenes, trimetallic nitride templated endohedral metallofullerenes, single-walled and multi-walled carbon nanotubes, branched and dendritic carbon nanotubes, gold nanorods, silver nanorods, single-walled and multi-walled boron/nitrate nanotubes, carbon nanotube peapods, carbon nanohorns, carbon nanohorn peapods, liposomes, nanoshells, dendrimers, microparticles, quantum dots, superparamagnetic nanoparticles, nanorods, cellulose nanoparticles, silicon, silica and polymer micro- and nano-spheres, silica-shells, biodegradable PLGA micro- and nano-spheres, gold particles, cerium oxide particles, zinc oxide particles, silver particles, aluminum particles, carbon particles, iron particles, iron oxide particles, adjuvant particles (e.
  • examples are nanoparticles with a hydrodynamic size of 5 nm to 999 nm (e.g., about 80 nm to about 200 nm, or about 90 nm to about 150 nm), as measured in an aqueous medium (such as PBS, Tris-buffer, or water).
  • the immunotherapeutic constructs have a hydrodynamic size of less than 150 nm, as measured in an aqueous medium (such as PBS, Tris-buffer, or water).
  • the immunotherapeutic constructs are microparticles with a hydrodynamic size of 1 micron to 1000 microns (e.g., 1 micron to 50 microns), as measured in an aqueous medium (such as PBS, Tris-buffer, or water).
  • an aqueous medium such as PBS, Tris-buffer, or water.
  • the therapeutic agent includes a siRNA, a miRNA, an antisense oligonucleotide, a mRNA, a DNA, a sgRNA (CRISPR-cas9 element), other oligonucleotide, other polynucleotide, a peptide, a protein, a chemotherapy drug, a toxin, an antioxidant, a small molecule inhibitor, an antibody, or a radio-therapeutic agent.
  • the therapeutic agent inhibits expression or an activity of STAT3, CD39, CD73, TGF- ⁇ , PD-L1, PD1, CTLA4, MIF, PLK1, HIF, NOX1-4, HER2, EGFR, BCL2, AKT1, HIF1-alpha, NOX1-4, AR, MYC, or MTDH.
  • the therapeutic agent is an anti-cancer agent including one or more of an antibiotic (e.g., docetaxel, doxorubicin, or mitoxantrone) a plant alkaloid (e.g., cabazitaxel), a PLK1 inhibitor, a mitotic kinase inhibitor, an immune checkpoint inhibitor (such as an antibody against PD-L1, PD1, or CTLA4), a platinum based chemotherapeutic agent, a small molecule HER2 inhibitor, or a HER2-specific antibody.
  • an antibiotic e.g., docetaxel, doxorubicin, or mitoxantrone
  • a plant alkaloid e.g., cabazitaxel
  • PLK1 inhibitor e.g., a mitotic kinase inhibitor
  • an immune checkpoint inhibitor such as an antibody against PD-L1, PD1, or CTLA4
  • platinum based chemotherapeutic agent e.g., a small molecule
  • mitotic kinase inhibitors include, but not limited to, inhibitors of at least one of a polo-like kinase (PLK), an Aurora kinase, cyclin-dependent kinase (CDK)1, CDK2, HASPIN, monopolar spindle 1 kinase (Mps1), or a NimA-related kinase (NEK).
  • the mitotic kinase inhibitor includes one or more of GSK461364, B12536, Tak960, NMS-P937, B16727 (volasertib), Chk 1 Kinase Inhibitor LY2603618, AU14022, YK-4-279, or PMN.
  • the adjuvant compound has immunostimulatory activity.
  • adjuvant compounds may include one or more of a CpG oligonucleotide, a DNA TLR agonist containing a CpG sequence, a non-CpG DNA TLR agonist, an RNA TLR agonist, an aluminum salt, an anti-CD40 antibody, a fusion protein, a cytokine, a small molecule TLR agonist, an oil- or surfactant-based adjuvant, a lipopolysaccharide, a plant extract, or a derivative thereof.
  • the adjuvant compound includes a CpG oligonucleotide, imiquimod, resiquimod, gardiquimod, poly IC, poly ICLC, dSLIM, or EnanDIM.
  • any of the provided immunotherapeutic construct embodiments may not include a tumor-specific antigen or ovalbumin.
  • compositions including: at least one immunotherapeutic construct as provided herein; and at least one pharmaceutically acceptable carrier, excipient, diluent, or mixture thereof.
  • One provided method of treating cancer includes administering to a subject with cancer an effective amount of an immunotherapeutic construct of any one of the described embodiments, or a composition including an immunotherapeutic construct, to reduce one or more symptoms of the cancer.
  • Another provided method embodiment is a method of treating a cell exhibiting symptoms of cancer, which method includes contacting the cell with a therapeutically effective amount of an immunotherapeutic construct of any one of the described embodiments, or a composition including an immunotherapeutic construct.
  • Another provided method embodiment is a method of treating a cell obtained from a subject exhibiting symptoms of cancer, which method includes contacting the cell with a therapeutically effective amount of an immunotherapeutic construct of any one of the described embodiments, or a composition including an immunotherapeutic construct.
  • the cell obtained from the subject is a cancer cell.
  • the cell is not a cancer cell; for instance, in some instances the non-cancer (e.g., normal) cell is an immunology cell.
  • the method further includes administering at least one treated cell back to the subject.
  • Another provided embodiment is a method of treating a subject diagnosed as having a hyperproliferative disease or condition or having a high-risk of developing such disease or condition, which method involves administering to the subject an effective amount of a composition that includes at least one immunotherapeutic construct as described herein.
  • the hyperproliferative disease or condition includes one or more of cancer, pre-cancer, or cancer metastasis.
  • the hyperproliferative disease in some cases includes one or more of melanoma, lung cancer, breast cancer, pancreatic cancer, brain cancer, prostate cancer, head and neck cancer, kidney cancer, colorectal cancer, lymphoma, colon cancer, or liver cancer.
  • the method enhances effect of an anti-cancer therapy in a subject in need thereof, which methods including administering to a subject in need thereof: an effective amount of an immunotherapeutic construct of any one of the described embodiments, or a composition including an immunotherapeutic construct; and at least one anti-cancer agent (e.g., a chemotherapeutic agent, a targeted therapeutic agent, or an immune checkpoint inhibitor).
  • an anti-cancer agent e.g., a chemotherapeutic agent, a targeted therapeutic agent, or an immune checkpoint inhibitor.
  • the method enhances a checkpoint blockade immunotherapy effect in a subject diagnosed as having a neoplasia, including administering to a subject in need thereof: an effective amount of an immunotherapeutic construct of any one of the described embodiments, or a composition including an immunotherapeutic construct; and at least one immune checkpoint inhibitor.
  • Yet another combination method is a method of enhancing radiation therapy effect in a subject diagnosed as having a neoplasia, including administering to a subject in need thereof: an effective amount of an immunotherapeutic construct of any one of the described embodiments, or a composition including an immunotherapeutic construct; and at least one radiation therapy.
  • the immunotherapeutic construct or composition and the second agent are administered sequentially or concurrently.
  • the term “enhancing,” in regards to the therapeutic effects of an anti-cancer therapy refers to an increase in the therapeutic effects of the anti-cancer therapy (e.g., treatment with an anti-cancer agent, radiation therapy, or checkpoint immunotherapy) above those normally obtained when the anti-cancer therapy is administered without the immunotherapeutic constructs of the invention. “An increase in the therapeutic effects” is manifested when there is an acceleration and/or increase in intensity and/or extend of the therapeutic effects obtained with an anti-cancer therapy. It also includes extension of the longevity of therapeutic benefits.
  • a lower dosage, frequency of dosing, or treatment of duration of the anti-cancer therapy is required to obtain the same benefits and/or effects when it is co-administered with the immunotherapeutic constructs provided by the present invention as when a higher dosage, frequency, or duration of the anti-cancer therapy is administered alone.
  • the enhancing effect preferably, but not necessarily, results in treatment of acute symptoms for which the anti-cancer therapy along is not effective or is less effective therapeutically.
  • Enhancement is achieved when there is at least a 10% increase (e.g., at least 25%, at least 50%, at least 75%, or at least 100%) in the therapeutic effects when an immunotherapeutic construct of the present invention is co-administered with an anti-cancer therapy compared with administration of the anti-cancer therapy alone.
  • a 10% increase e.g., at least 25%, at least 50%, at least 75%, or at least 100%
  • Administering an immunotherapeutic construct in any of the described method embodiments may include one or more of: injection directly into or around a tumor, lesion, or resected tumor area in the subject; or systemic injection in the subject; or topical application to the subject; or inhalation; or implantation device; or microneedle application to the subject.
  • the subject (being treated, to which a construct or composition is being administered, or from which a cell is obtained) is a mammal; for instance, in certain embodiments the mammal is a human.
  • kits including an immunotherapeutic construct described herein and at least one anti-cancer agent.
  • the anti-cancer agent is a chemotherapeutic agent, a targeted therapeutic agent, or an immune check point inhibitor.
  • immunotherapeutic constructs that include an engineered particle which co-delivers adjuvant(s) and therapeutically active agent(s) to cancer cells.
  • Embodiments provide therapeutically active agent(s) that induce antigen release (specifically, tumor antigen release) and/or modulate an immunosuppressive environment (such as a tumor microenvironment).
  • These immunotherapeutic constructs boost the CD8+ T cell repertoire and induce systemic anti-tumor immunotherapy effects, without any need to know or identify which antigen(s) are associated with the cancer being treated.
  • This strategy will have many key features; they are efficacious, personalized, safe because of local delivery, durable because they train and harness body immune cells to attack cancer with memory effects, inexpensive (e.g., low dosage and low number of dosings are required), and applicable to many types of cancer.
  • each component in an immunotherapeutic construct may vary, depending in the embodiment.
  • any individual component may make up 0.001% to 80% by weight, 0.01% to 75% by weight, 0.5 to 50% by weight, 0.5 to 10% by weight, 0.5 to 5% by weight, 1 to 10% by weight, or 2 to 4% by weight, of the immunotherapeutic construct.
  • the therapeutic agent comprises an oligonucleotide (e.g., siRNA or any other oligonucleotide described herein), and the oligonucleotide makes up 0.5 to 30% by weight of the immunotherapeutic construct, e.g., 0.5 to 10%, 1 to 5%, 5 to 15%, or 10 to 30%.
  • the therapeutic agent comprises an anti-cancer agent (e.g., a small molecule inhibitor or any other anti-cancer agent described herein), and the anti-cancer agent makes up 0.1 to 30% by weight of the immunotherapeutic construct, e.g., 0.5 to 10%, 1 to 5%, 5 to 15%, or 10 to 30%.
  • the therapeutic agent comprises an antibody, and the antibody makes up 0.1 to 30% by weight of the immunotherapeutic construct, e.g., 0.5 to 10%, 1 to 5%, 5 to 15%, or 10 to 30%.
  • therapeutically active agents e.g., therapeutic oligonucleotides, including siRNA, miRNA, antisense oligonucleotides, sgRNA-cas9, DNA, and mRNA, as well as small molecule inhibitors, chemotherapeutics, antibodies, chemical agents, etc.
  • the active agents kill cancer cells, thereby releasing tumor antigens, while the co-delivered adjuvants (e.g., CpG, R848, poly I:C, etc.) prime and activate adaptive immune cells against the released tumor antigens.
  • co-delivered adjuvants e.g., CpG, R848, poly I:C, etc.
  • the activated effector cells can recognize and attack tumors at any sites in the body (including sites remote from localized delivery of the immunotherapeutic construct) as well as reducing or even preventing the spread, recurrence, or the development of new tumors harboring one or more of the same tumor antigens as the treated tumors.
  • dose of therapeutic agents on the immunotherapeutic construct may be adjusted to lessen toxicity to beneficial immune cells.
  • therapeutic agents that affect cancer's viability without harming immune cells are utilized on the immunotherapeutic construct.
  • therapeutically active agents e.g., siRNAs, inhibitors, or other drugs against STAT3, CD39, CD73, IDO-6, PD-L1, TGF- ⁇ , antioxidants, etc.
  • the delivery vehicle e.g., particle
  • therapeutically active agents may also be loaded on/within the delivery vehicle (e.g., particle) to modulate immunosuppressive tumor microenvironment, allowing priming and activation of immune cells to effectively attack cancer cells, utilizing antigens that are already in the tumor or whose release are triggered by the immunotherapeutic construct.
  • Therapeutic agents can target, as an example, STAT3, IDO-1, TGF- ⁇ , CD47, NOX1-5, HSP47, XBP1, BCL2, BCL-XL, AKT1, AKT2, AKT3, MYC, HER2, HER3, AR, Survivin, GRB7, EPS8L1, RRM2, PKN3, EGFR, IRE1-alpha, VEGF-R1, RTP801, proNGF, Keratin K6A, LMP2, LMP7, MECL1, HIF1 ⁇ , Furin, KSP, eiF-4E, p53, ⁇ -catenin, ApoB, PCSK9, SNALP, CD39, CD73, PD-L1, PD-1, CTLA-4, MIF, VEGF, PIGF, CXCR4, CCR2, PLK1, MTDH, Twist, Lcn2, IL-6, IL-10, SOCS1, TRAIL, p65, and mitotic kinases (e.g.,
  • Therapeutic agents can target other immunosuppressive genes known in the art (e.g., Liu et al., Database , bax094, 2017; Rabinovich et al., Annu Rev Immunol, 25:267, 2010). Therapeutic agents can also inhibit the activity of immune checkpoints known in the art.
  • the immune checkpoints beneficial for cancer treatment, when inhibited non-exhaustively include PD-L1, PD-1, CTLA-4, LAG-3, TIM-3, B7-H3, VISTA, A2AR, IDO, etc. (Khair et al., Frontiers Immunology, 10:453, 2019). Altogether, immunotherapeutic construct will cause long-lasting immune-mediated anti-cancer effects. Memory adaptive immunity may also be established.
  • the immunotherapeutic constructs are used to activate an immune response. Such embodiments are not limited to a particular manner of activating an immune response.
  • Therapeutic oligonucleotides Different types can be used and non-exhaustively include siRNA, miRNA, antisense oligonucleotide, ribozyme, aptamer, DNA, mRNA, sgRNA (for CRISPR), and CRISPR-cas9 elements. In other words, any chains of nucleotides can be utilized in this art as long as they can specifically modulate (interfere or boost) the action or synthesis of certain genes and proteins.
  • Each particular oligonucleotide may have a single or multiple targets. Examples of gene/protein targets of interest to the invention include immune checkpoints, transcription factors, phosphatases, kinases, etc.
  • Specific targets include STAT3, IDO-1, TGF- ⁇ , CD47, NOX1-5, HSP47, XBP1, BCL2, BCL-XL, AKT1, AKT2, AKT3, MYC, HER2, HER3, AR, Survivin, GRB7, EPS8L1, RRM2, PKN3, EGFR, IRE1-alpha, VEGF-R1, RTP801, proNGF, Keratin K6A, LMP2, LMP7, MECL1, HIF1 ⁇ , Furin, KSP, eiF-4E, p53, ⁇ -catenin, ApoB, PCSK9, SNALP, CD39, CD73, PD-L1, PD-1, CTLA-4, MIF, VEGF, PIGF, CXCR4, CCR2, PLK1, MTDH, Twist, Lcn2, IL-6, IL-10, SOCS1, TRAIL, p65, and mitotic kinases (e.g., PLK1, PLK2,
  • Therapeutic oligonucleotides can also target other Immunosuppressive genes known in the arts (e.g., Liu et al., Database , bax094, 2017; Rabinovich et al., Annu Rev Immunol, 25:267, 2010).
  • Therapeutic oligonucleotides can inhibit the expression and activity of immune checkpoints known in the art.
  • the immune checkpoints beneficial for cancer treatment, when inhibited non-exhaustively include PD-L1, PD-1, CTLA-4, LAG-3, TIM-3, B7-H3, VISTA, A2AR, IDO, etc. (Khair et al., Frontiers Immunology, 10:453, 2019).
  • Therapeutic oligonucleotides can also contain two strands that target two genes (such as siRNA against BLC2 and AKT1, siRNA against AR and MYC). They can also contain immunostimulatory sequences/elements that can thus simultaneously boost the immune response and regulate expression of target genes. They can also be designed to target the aforementioned genes that have mutations.
  • the immunotherapeutic constructs include as an active agent an oligonucleotide that mediates RNA interference.
  • RNA interference is a highly conserved mechanism triggered by double-stranded RNA (dsRNA) and able to downregulate transcript of genes homologous to the dsRNA.
  • dsRNA double-stranded RNA
  • the dsRNA is first processed by Dicer into short duplexes of 21-23 nucleotides, called short interfering RNAs (siRNAs).
  • siRNAs short interfering RNAs
  • RISC RNA-induced silencing complex
  • siRNA or “small-interfering ribonucleic acid” refers to two strands of ribonucleotides which hybridize along a complementary region under physiological conditions.
  • the siRNA molecules include a double-stranded region which is substantially identical to a region of the mRNA of the target gene. A region with 100% identity to the corresponding sequence of the target gene is suitable. This state is referred to as “fully complementary”. However, the region may also contain one, two or three mismatches as compared to the corresponding region of the target gene, depending on the length of the region of the mRNA that is targeted, and as such may be not fully complementary.
  • Methods to analyze and identify siRNAs with sufficient sequence identity in order to effectively inhibit expression of a specific target sequence are known in the art.
  • a suitable mRNA target region would be the coding region. Also suitable are untranslated regions, such as the 5′-UTR, the 3′-UTR, and splice junctions as long as the regions are unique to the mRNA target.
  • siRNA encapsulated within or associated with immunotherapeutic constructs are utilized in methods and systems involving RNA interference. Such embodiments are not limited to a particular size or type of siRNA molecule.
  • the length of the region of the siRNA complementary to the target may be from 15 to 100 nucleotides, 18 to 25 nucleotides, 20 to 23 nucleotides, or more than 15, 16, 17 or 18 nucleotides. Where there are mismatches to the corresponding target region, the length of the complementary region is generally required to be somewhat longer.
  • the siRNA delivery approach using immunotherapeutic constructs disclosed herein can be used to inhibit production of any gene of interest.
  • Specific targets include STAT3, IDO-1, TGF- ⁇ , CD47, NOX1-5, HSP47, XBP1, BCL2, BCL-XL, AKT1, AKT2, AKT3, MYC, HER2, HER3, AR, Survivin, GRB7, EPS8L1, RRM2, PKN3, EGFR, IRE1-alpha, VEGF-R1, RTP801, proNGF, Keratin K6A, LMP2, LMP7, MECL1, HIF1 ⁇ , Furin, KSP, eiF-4E, p53, ⁇ -catenin, ApoB, PCSK9, SNALP, CD39, CD73, PD-L1, PD-1, CTLA-4, MIF, VEGF, PIGF, CXCR4,
  • siRNA can be directed to a variant or mutated gene, rather than a wildtype gene.
  • Such embodiments are not limited to a particular manner of assessing the delivery profile of the siRNA in vitro and/or in vivo.
  • labelling the siRNA molecules with an imaging agent e.g., fluorescent dye FITC, RITC, CyTM dyes, Dylight® dyes, or Alexa Fluor® dyes
  • an imaging agent e.g., fluorescent dye FITC, RITC, CyTM dyes, Dylight® dyes, or Alexa Fluor® dyes
  • a radiotracer permits visualization of the biodistribution of siRNA molecules at the organ level and also the intracellular delivery profile.
  • RT-PCR, FISH, IHC, flow cytometry, and western blot are used to analyze the target protein at the mRNA level and protein level, respectively.
  • the present disclosure provides methods for inhibiting a target gene in a cell including introducing into the cell a siRNA capable of inhibiting the target gene by RNA interference, wherein the siRNA includes two RNA strands that are complementary to each other, wherein the siRNA is loaded on to an immunotherapeutic construct.
  • the siRNA is modified with cholesterol at the 3′ sense strand.
  • the cell is within a human being or an animal subject (e.g., horses, dogs, cats, or other domestic, farm, or other animals with cancer).
  • miRNAs or miRNA mimics are short, non-coding RNAs that can target and substantially silence protein coding genes through 3′-UTR elements. Important roles for miRNAs in numerous biological processes have been established, but comprehensive analyses of miRNA function in complex diseases are lacking. miRNAs are initially transcribed as primary miRNAs (pri-miRNAs) that are then cleaved by the nuclear RNAses Drosha and Pasha to yield precursor-miRNAs (pre-miRNAs).
  • pri-miRNAs primary miRNAs
  • pre-miRNAs precursor-miRNAs
  • RNA Induced Silencing Complex that includes the enzymes dicer and Argonaute (Ago).
  • the mature miRNAs ( ⁇ 17-24nt) direct RISC to specific target sites located within the 3′UTR of target genes.
  • miRNAs represses translation through mRNA decay, translational inhibition and/or sequestration into processing bodies (P-bodies) (Eulalio et al., Cell, 132:9-14, 2008; Behm-Ansmant et al., Cold Spring Harb. Symp. Quant. Biol., 71:523-530, 2006; Chu and Rana, Plos. Biology., 4:e210, 2006). Recent estimates find that over 60% of protein coding genes carry 3′-UTR miRNA target sites (Friedman et al., Genome Res., 19:92-105, 2009).
  • miRNAs act as key regulators of processes as diverse as early development (Reinhart et al., Nature, 403:901-906, 2000), cell proliferation and cell death (Brennecke et al., Cell, 113(1):25-36, 2003), apoptosis and fat metabolism (Xu et al., Curr. Biol., 13(9):790-795, 2003), and cell differentiation (Chen et al., Mol. Microbiol., 53843-856, 2004; Dostie et al., RNA - A Publication of the RNA Society, 9:180-186, 2003).
  • studies of miRNA expression in chronic lymphocytic leukemia (Calin et al., Proc.
  • miRNAs thus far observed have usually been 21-22 nucleotides in length and they arise from longer precursors, which are transcribed from non-protein-encoding genes. Reviewed in Carrington and Ambros (Science, 301(5631):336-338, 2003). The precursors form structures that fold back on each other in self-complementary regions; they are then processed by the nuclease Dicer in animals (or DCL1 in plants). miRNA molecules interrupt translation through precise or imprecise base-pairing with their targets.
  • a miRNA may be used as a component of a provided immunotherapeutic construct, therapeutically, or administered to a subject, such as a human patient, to treat a disease such as, e.g., cancer; alternately, in some embodiments, a nucleic acid that is complementary to the miRNA may be therapeutically administered to a subject in vivo or used in vitro to generate the desired therapeutic outcome (e.g., miRNA-142-3p, miRNA-142-3p, miRNA-124, or miRNA-138). In this way, the complementary nucleic acid may be used as a template to generate the desired therapeutic miRNA (e.g., miRNA-142-3p, miRNA-142-3p, miRNA-124, or miRNA-138).
  • the desired therapeutic miRNA e.g., miRNA-142-3p, miRNA-142-3p, miRNA-124, or miRNA-138.
  • STAT3 refers to the protein “Signal transducer and activator of transcription 3” and homologs thereof; the term includes the human protein, whether wildtype or mutant forms of the protein.
  • STAT3 refers to the protein associated with Entrez Gene 6774, OMIM 102582, UniProt P40763, and/or RefSeq (protein) NP 003141 (which refer to the protein, and associated nucleic acids, known as of the date of filing of this application).
  • Phosphorylated STAT3 refers to a STAT3 protein that is phosphorylated and activated by the phosphorylation.
  • a phosphorylated STAT3 is phosphorylated on tyrosine 705 or the residue corresponding to tyrosine 705 in homologs.
  • activation of STAT3 means the STAT3 is capable of activating transcription of other genes.
  • activated STAT3 is phosphorylated on tyrosine 705, or the residue corresponding to tyrosine 705, forms dimers (e.g. homodimers or heterodimers), translocates to the nucleus, and/or activates transcription.
  • activated STAT3 forms homodimers. Examples of proteins that phosphorylate STAT3 and thereby activate STAT3 include JAK2, EGFR, c-MET, and PDGF-R.
  • anti-cancer agent is used in accordance with its plain ordinary meaning and refers to a composition (e.g. compound, drug, antagonist, inhibitor, modulator) having antineoplastic properties or the ability to inhibit the growth or proliferation of cells.
  • an anti-cancer agent is a chemotherapeutic agent.
  • the anti-cancer agent is a targeted therapeutic agent.
  • the anticancer agent is an immune checkpoint inhibitor.
  • the anti-cancer agent is an agent identified herein having utility in methods of treating cancer.
  • the anti-cancer agent is an agent approved by the FDA or similar regulatory agency of a country other than the USA, for treating cancer.
  • anti-cancer agents include, but are not limited to, MEK (e.g., MEK1, MEK2, or MEK1 and MEK2) inhibitors (e.g., XL518, CI-1040, PD035901, selumetinib/AZD6244, GSK1120212/trametinib, GDC-0973, ARRY-162, ARRY-300, AZD8330, PD0325901, U0126, PD98059, TAK-733, PD318088, AS703026, BAY 869766, PD184352, SB239063, BAY 43-9006); alkylating agents such as nitrogen mustards (e.g., mechloroethamine, cyclophosphamide, uramustine, chlorambucil, melphalan, ifosfamide), ethylenimine and methylmelamines (e.g., hexamethlymelamine and thiotepa),
  • immunotherapeutic constructs described herein can be co-administered with conventional immunotherapeutic agents including, but not limited to, immunostimulants (e.g., Bacille Calmette-Guerin (BCG), levamisole, interleukin-2, alpha-interferon, etc.), therapeutic monoclonal antibodies (e.g., anti-CD20, anti-HER2, anti-CD52, anti-HLA-DR, and anti-VEGF monoclonal antibodies), immunotoxins (e.g., anti-CD33 monoclonal antibody-calicheamicin conjugate, anti-CD22 monoclonal antibody-pseudomonas exotoxin conjugate, etc.), immune-checkpoint inhibitors (e.g., anti-CTLA4, anti-PD1, antiPD-L1 antibodies), and radioimmunotherapy (e.g., anti-CD20 monoclonal antibody conjugated to 111 In, 90 Y, or 131 I, etc.).
  • immunostimulants e.g.,
  • the immunotherapeutic constructs described herein can be co-administered with conventional radiotherapeutic agents including, but not limited to, radionuclides such as 47 Sc, 65 Cu, 67 Cu, 89 Sr, 86 Y, 87 Y, 80 Y, 105 Rh, 111 Ag, 111 In, 117 mSn, 149 Pm, 153 Sm, 166 Ho, 177 Lu, 186 Re, 188 Re, 211 At and 212 Bi.
  • radiotherapeutic agents can also be loaded directly onto the immunotherapeutic constructs to enhance the therapeutic effect, reduce toxicity, and reduce administration time.
  • oligonucleotides antibodies and small molecule inhibitors interfering with or boosting the activity of target genes and proteins can be used in a similar manner.
  • PD-1 antibody instead of siRNA against PD-1, PD-1 antibody can also be loaded on the nanoparticle as the therapeutic agent component in the herein-provided immunotherapeutic construct.
  • the immunotherapeutic constructs provided herein include at least one adjuvant component, e.g., contained within or otherwise associated with the delivery vehicle.
  • the immunotherapeutic construct embodiments are not limited to a particular type of adjuvant, though specific examples are provided herein.
  • Adjuvants may also be part of or conjugated with therapeutic agents. For example, siRNA that knocks down a target gene can be designed to contain immune-stimulatory sequence.
  • the at least one adjuvant makes up 0.5 to 20% by weight of the immunotherapeutic construct.
  • adjuvants are any substance whose admixture into a vaccine composition increases or otherwise modifies the immune response to a (cancer) antigen.
  • adjuvants with immunostimulatory activity.
  • Adjuvants induce a nonspecific activation of the immune system, unless they are associated with antigens (e.g., adjuvants in vaccines).
  • antigens e.g., adjuvants in vaccines.
  • the ability of an adjuvant to increase the immune response to an antigen is typically manifested by a significant increase in immune-mediated reaction, or reduction in disease symptoms.
  • an increase in humoral immunity is typically manifested by a significant increase in the titer of antibodies raised to the antigen
  • an increase in T-cell activity is typically manifested in increased antigen-specific T cell proliferation, death of target cells, or cytokine secretion.
  • An adjuvant may also alter an immune response, for example, by changing a primarily humoral or Th2 response into a primarily cellular, or Th1 response.
  • Suitable adjuvants include TLR-binding DNA substituents such as CpG oligonucleotides (e.g., ISS 1018; Amplivax; CpG ODN 7909, CpG ODN 1826, CpG ODN D19, CpG ODN 1585, CpG ODN 2216, CpG ODN 2336, ODN 1668, ODN 1826, ODN 2006, ODN 2007, ODN 2395, ODN M362, and SD-101), DNA TLR agonists that contain a CpG sequence (e.g., dSLIM), non-CpG DNA TLR agonists (e.g., EnanDIM), and cationic peptide-conjugated CpG oligonucleotides (e.g., IC30, IC31); RNA TLR agonists (e.g., Poly I:C and Poly-ICLC); aluminum salts (e.g., aluminum hydroxide, aluminum phosphate, aluminum chlor
  • cytokines may be used.
  • Several cytokines have been directly linked to influencing dendritic cell migration to lymphoid tissues (e.g., TNF-alpha), accelerating the maturation of dendritic cells into efficient antigen-presenting cells for T-lymphocytes (e.g., GM-CSF, IL-1 and IL-4) (U.S. Pat. No. 5,849,589) and acting as immunoadjuvants (e.g., IL-12) (Gabrilovich et al., J Immunother Emphasis Tumor Immunol . (6):414-418, 1996).
  • TLRs Toll like receptors
  • PRRs pattern recognition receptors
  • PAMPS pathogen-associated molecular patterns
  • the adjuvant includes a CpG oligonucleotide.
  • CpG immuno-stimulatory oligonucleotides have also been reported to enhance the effects of adjuvants in a vaccine setting. Without being bound by any particularly mechanistic theory, CpG oligonucleotides act at least in part by activating the innate (non-adaptive) immune system via Toll-like receptors (TLR), mainly TLR9.
  • TLR Toll-like receptors
  • CpG triggered TLR9 activation enhances antigen-specific humoral and cellular responses to a wide variety of antigens, including peptide or protein antigens, live or killed viruses, dendritic cell vaccines, autologous cellular vaccines and polysaccharide conjugates in both prophylactic and therapeutic vaccines. More importantly, it enhances dendritic cell maturation and differentiation, resulting in enhanced activation of T H 1 cells and strong cytotoxic T-lymphocyte (CTL) generation, even in the absence of CD4 T-cell help.
  • CTL cytotoxic T-lymphocyte
  • the T H 1 bias induced by TLR9 stimulation is maintained even in the presence of vaccine adjuvants such as alum or incomplete Freund's adjuvant (IFA) that normally promote a T H 2 bias.
  • vaccine adjuvants such as alum or incomplete Freund's adjuvant (IFA) that normally promote a T H 2 bias.
  • CpG oligonucleotides show even greater adjuvant activity when formulated or co-administered with other adjuvants or in formulations such as microparticles, nano particles, lipid emulsions or similar formulations, which are especially necessary for inducing a strong response when the antigen is relatively weak. They also accelerate the immune response and enabled the antigen doses to be reduced by two orders of magnitude, with comparable antibody responses to the full-dose vaccine without CpG in some experiments (Krieg, Nature Reviews, Drug Discovery, 5:471-484, 2006).
  • 6,406,705 describes the combined use of CpG oligonucleotides, non-nucleic acid adjuvants and an antigen to induce an antigen-specific immune response.
  • a commercially available CpG TLR9 agonist is dSLIM (double Stem Loop Immunomodulator) by Mologen (Berlin, GERMANY).
  • Other TLR binding molecules such as RNA binding TLR 7, TLR 8 and/or TLR 9 may also be used.
  • Xanthenone derivatives such as, for example, vadimezan or AsA404 (also known as 5,6-dimethylaxanthenone-4-acetic acid (DMXAA)), may also be used as adjuvants according to embodiments of the invention. Alternatively, such derivatives may also be administered in parallel to the vaccine of the invention, for example via systemic or intratumoral delivery, to stimulate immunity at the tumor site.
  • DMXAA 5,6-dimethylaxanthenone-4-acetic acid
  • xanthenone derivatives act by stimulating interferon (IFN) production via the stimulator of IFN gene (STING) receptor (see e.g., Conlon et al., J Immunology, 190:5216-5225, 2013; and Kim et al., ACS Chem Biol, 8:1396-1401, 2013).
  • IFN interferon
  • STING stimulator of IFN gene
  • useful adjuvants include chemically modified CpGs (e.g. CpR, Idera), Poly(I:C) (e.g.
  • polyi:CI2U non-CpG bacterial DNA or RNA as well as immunoactive small molecules and antibodies such as cyclophosphamide, sunitinib, bevacizumab, CelebrexTM, NCX-4016, sildenafil, tadalafil, vardenafil, sorafinib, XL-999, CP-547632, pazopanib, ZD2171, AZD2171, ipilimumab, tremelimumab, and SC58175, which may act therapeutically and/or as an adjuvant.
  • the amounts and concentrations of adjuvants and additives useful in the context of the present invention can readily be determined by the skilled artisan without undue experimentation. Additional adjuvants include colony-stimulating factors, such as Granulocyte Macrophage Colony Stimulating Factor (GM-CSF, sargramostim).
  • GM-CSF Granulocyte Macrophage Colony St
  • Poly-ICLC is a synthetically prepared double-stranded RNA including polyl and polyC strands of average length of 5000 nucleotides, which has been stabilized to thermal denaturation and hydrolysis by serum nucleases by the addition of poly-lysine and carboxymethylcellulose.
  • the compound activates TLR3 and the RNA helicase-domain of MDAS, both members of the PAMP family, leading to DC and natural killer (NK) cell activation and production of a “natural mix” of type I interferons, cytokines, and chemokines.
  • poly-ICLC exerts a more direct, broad host-targeted anti-infectious and possibly antitumor effect mediated by the two IFN-inducible nuclear enzyme systems, the 2′5′-OAS and the PI/eIF2a kinase, also known as the PKR (4-6), as well as RIG-I helicase and MDAS.
  • immunological adjuvants examples include TLR ligands, C-Type Lectin Receptor ligands, NOD-Like Receptor ligands, RLR ligands, and RAGE ligands.
  • TLR ligands can include lipopolysaccharide (LPS) and derivatives thereof, as well as lipid A and derivatives thereof including monophosphoryl lipid A (MPL), glycopyranosyl lipid A, PET-lipid A, and 3-O-desacyl-4′-monophosphoryl lipid A.
  • LPS lipopolysaccharide
  • MPL monophosphoryl lipid A
  • glycopyranosyl lipid A glycopyranosyl lipid A
  • PET-lipid A and 3-O-desacyl-4′-monophosphoryl lipid A.
  • the immunological adjuvant is MPL.
  • the immunological adjuvant is LPS.
  • TLR ligands can also include TLR3 ligands (e.g., polyinosinic-polycytidylic acid (poly(I:C)), TLR7 ligands (e.g., imiquimod and resiquimod), and TLR9 ligands.
  • TLR3 ligands e.g., polyinosinic-polycytidylic acid (poly(I:C)
  • TLR7 ligands e.g., imiquimod and resiquimod
  • TLR9 ligands e.g., imiquimod and resiquimod
  • TLR-binding DNA substituent refers to a substituent or moiety capable of binding to a toll-like receptor (“TLR”), including at least one deoxyribonucleic acid.
  • TLR toll-like receptor
  • a TLR-binding DNA substituent is a nucleic acid.
  • the TLR-binding DNA substituent includes at least one nucleic acid analog.
  • the TLR-binding DNA substituent includes at least one nucleic acid analog having an alternate backbone (e.g. phosphodiester derivative (e.g.
  • a TLR-binding DNA substituent includes DNA.
  • all nucleotide sugars in a TLR-binding DNA substituent are deoxyribose (e.g., all nucleotides are DNA).
  • a TLR-binding DNA substituent includes or is DNA having internucleotide linkages selected from phosphodiesters and phosphodiester derivatives (e.g. phosphoramidate, phosphorodiamidate, phosphorothioate, phosphorodithioate, phosphonocarboxylic acids, phosphonocarboxylates, phosphonoacetic acid, phosphonoformic acid, methyl phosphonate, boron phosphonate, O-methylphosphoroamidite, or combinations thereof).
  • a TLR-binding DNA substituent includes DNA having internucleotide linkages selected from phosphodiesters and phosphorothioates.
  • a TLR-binding DNA substituent includes or is DNA having backbone linkages selected from phosphodiesters and phosphorodithioates. In embodiments, a TLR-binding DNA substituent includes or is DNA including phosphodiester backbone linkages. In embodiments, a TLR-binding DNA substituent includes or is DNA including phosphorothioate backbone linkages. In embodiments, a TLR-binding DNA substituent includes or is DNA including phosphorodithioate backbone linkages. In embodiments, a TLR-binding DNA substituent preferentially binds TLR9 over other TLR. In embodiments, a TLR-binding DNA substituent specifically binds TLR9.
  • a TLR-binding DNA substituent specifically binds TLR3. In embodiments, a TLR-binding DNA substituent specifically binds TLR7. In embodiments, a TLR-binding DNA substituent specifically binds TLR8. In embodiments, a TLR-binding DNA substituent specifically binds a cellular sub-compartment (e.g. endosome) associated TLR (e.g. TLR3, TLR7, TLR8, or TLR9). In embodiments, a TLR-binding DNA substituent includes or is a G-rich oligonucleotide.
  • a TLR-binding DNA substituent includes a CpG motif, wherein C and G are nucleotides and p is the phosphate connecting the C and G. In embodiments, the CpG motif is unmethylated.
  • a TLR-binding DNA substituent is a Class A CpG oligodeoxynucleotide (ODN).
  • a TLR-binding DNA substituent is a Class B CpG oligodeoxynucleotide (ODN).
  • a TLR-binding DNA substituent is a Class C CpG oligodeoxynucleotide (ODN).
  • a TLR-binding DNA substituent e.g., TLR9-binding DNA substituent
  • TLR9-binding DNA substituent include deoxyribonucleic acids with A, G, C, or T bases and phosphodiester linkages and/or phosphodiester derivative linkages (e.g., phosphorothioate linkage(s)).
  • CpG motif refers to a 5′ C nucleotide connected to a 3′ G nucleotide through a phosphodiester internucleotide linkage or a phosphodiester derivative internucleotide linkage.
  • a CpG motif includes a phosphodiester internucleotide linkage.
  • a CpG motif includes a phosphodiester derivative internucleotide linkage.
  • Class A CpG ODN or “A-class CpG ODN” or “D-type CpG ODN” or “Class A CpG DNA sequence” is used in accordance with its common meaning in the biological and chemical sciences and refers to a CpG motif including oligodeoxynucleotide including one or more of poly-G sequence at the 5′, 3′, or both ends; an internal palindrome sequence including CpG motif; or one or more phosphodiester derivatives linking deoxynucleotides.
  • a Class A CpG ODN includes poly-G sequence at the 5′, 3′, or both ends; an internal palindrome sequence including CpG motif; and one or more phosphodiester derivatives linking deoxynucleotides.
  • the phosphodiester derivative is phosphorothioate. Examples of Class A CpG ODNs include ODN D19, ODN 1585, ODN 2216, and ODN 2336.
  • Class B CpG ODN or “B-class CpG ODN” or “K-type CpG ODN” or “Class B CpG DNA sequence” are used in accordance with their common meaning in the biological and chemical sciences, and refer to a CpG motif including oligodeoxynucleotide including one or more of a 6mer motif including a CpG motif; phosphodiester derivatives linking all deoxynucleotides.
  • a Class B CpG ODN includes one or more copies of a 6mer motif including a CpG motif and phosphodiester derivatives linking all deoxynucleotides.
  • the phosphodiester derivative is phosphorothioate.
  • a Class B CpG ODN includes one 6mer motif including a CpG motif. In embodiments, a Class B CpG ODN includes two copies of a 6mer motif including a CpG motif. In embodiments, a Class B CpG ODN includes three copies of a 6mer motif including a CpG motif. In embodiments, a Class B CpG ODN includes four copies of a 6mer motif including a CpG motif. Examples of Class B CpG ODNs include ODN 1668, ODN 1826, ODN 2006, and ODN 2007.
  • Class C CpG ODN or “C-class CpG ODN” or “C-type CpG DNA sequence” are used in accordance with their common meaning in the biological and chemical sciences and refers to an oligodeoxynucleotide including a palindrome sequence including a CpG motif and phosphodiester derivatives (phosphorothioate) linking all deoxynucleotides.
  • Class C CpG ODNs include ODN 2395 and ODN M362.
  • targeting moieties can be included, e.g., loaded into, attached to the surface of, and/or enclosed within the delivery vehicle.
  • the targeting moiety is displayed on the exterior surface of the delivery vehicle.
  • targeting moieties allow specific cell targeting (e.g., preventing delivery of toxic therapeutic agents to immune cells, enriching/targeting delivery of therapeutic agents with cell-specific function).
  • targeting moieties may also have therapeutic activity and thus also serve as therapeutic agents of the herein-provided immunotherapeutic construct (e.g., PD-L1 antibody). Such targeting moieties may be particularly beneficial for systemic delivery.
  • Exemplary targeting molecules include proteins, peptides, ligands, nucleic acids, lipids, saccharides, antibodies, aptamers, affibody molecules, small molecules, or polysaccharides that bind to one or more targets associated with an organ, tissue, cell, or extracellular matrix, or specific type of tumor or infected cell.
  • the degree of specificity with which the delivery vehicles are targeted can be modulated through the selection of a targeting molecule with the appropriate affinity and specificity.
  • antibodies are very specific. These can be polyclonal, monoclonal, fragments, recombinant, or single chain, many of which are commercially available or readily obtained using standard techniques.
  • T-cell specific molecules, antigens, and tumor targeting molecules can be bound to the surface of the immunotherapeutic constructs.
  • the targeting molecules may be conjugated to the terminus of one or more PEG chains present on the surface of the particle.
  • the targeting moiety is an antibody or antigen binding fragment (e.g., single chain variable fragments) thereof that specifically recognizes a cell or tumor marker that is present exclusively or in elevated amounts on a target cell, such as a malignant cell (e.g., a tumor antigen).
  • a target cell such as a malignant cell (e.g., a tumor antigen).
  • Suitable targeting molecules that can be used to direct immunotherapeutic constructs to cells and tissues of interest, as well as methods of conjugating target molecules to nanoparticles, are known in the art. See, for example, Ruoslahti et al. ( Nat. Rev. Cancer, 2:83-90, 2002).
  • Exemplary tumor antigens that can be targeted with antigen binding molecules such as antibodies are discussed above with respect to vaccine antigens.
  • immunotherapeutic constructs can be conjugated with a targeting moiety to enrich the delivery of therapeutic agent and adjuvant to only cancer cells.
  • a targeting moiety to enrich the delivery of therapeutic agent and adjuvant to only cancer cells. Examples include antibodies against HER2, EGFR, PSMA, PD-L1, etc. that are expressed or optionally overexpressed on cancer cells.
  • immunotherapeutic constructs can be conjugated with a targeting moiety to enrich the delivery of therapeutic agent and adjuvant to only immune cells.
  • Targeting molecules can also include neuropilins and endothelial targeting molecules, integrins, selectins, adhesion molecules, bone targeting molecules such as zoledronic acid and alendronic acid (e.g., to target cancer metastasized to bone), stroma, and fibroblast targeting molecules.
  • the targeting moiety targets the immunotherapeutic construct to antigen-presenting cells (APCs), and particularly to a subclass of APCs known as dendritic cells.
  • APCs antigen-presenting cells
  • dendritic cells express a number of cell surface receptors that can mediate endocytosis.
  • immunotherapeutic construct enhances the activity of DC to process tumor antigen and thus better trigger in situ tumor vaccination. Targeted delivery to DC may be performed. Targeting exogenous antigens to internalizing surface molecules on systemically-distributed antigen presenting cells facilitates uptake of the particle and can overcomes a major rate-limiting step in the therapy.
  • Dendritic cell targeting molecules include monoclonal or polyclonal antibodies or fragments thereof that recognize and bind to epitopes displayed on the surface of dendritic cells. Dendritic cell targeting molecules also include ligands which bind to a cell surface receptor on dendritic cells.
  • One such receptor, the lectin DEC-205 has been used in vitro and in mice to boost both humoral (antibody-based) and cellular (CD8 T cell) responses by 2-4 orders of magnitude (Hawiger et al., J. Exp. Med., 194(6):769-79, 2001; Bonifaz et al., J. Exp.
  • a variety of other endocytic receptors including a mannose-specific lectin (mannose receptor) and IgG Fc receptors, have also been targeted in this way with similar enhancement of antigen presentation efficiency.
  • Other suitable receptors which may be targeted include DC-SIGN, 33D1, SIGLEC-H, DCIR, CD11c, heat shock protein receptors and scavenger receptors.
  • Targeting moieties for these receptors can be attached to the immunotherapeutic constructs for their preferential uptake into immune cells that express these receptors.
  • Example is mannose attached on the immunotherapeutic constructs for targeted delivery to macrophages and DCs that have high levels of mannose receptors.
  • TLRs toll-like receptors
  • PAMPs pathogen-associated molecular patterns
  • PAMPs conjugated to the particle surface or co-encapsulated include unmethylated CpG DNA (bacterial), double-stranded RNA (viral), lipopolysaccharide (bacterial), peptidoglycan (bacterial), lipoarabinomannin (bacterial), zymosan (yeast), mycoplasmal lipoproteins such as MALP-2 (bacterial), flagellin (bacterial) poly(inosinic-cytidylic) acid (bacterial), lipoteichoic acid (bacterial) or imidazoquinolines (synthetic).
  • CpG DNA bacterial
  • double-stranded RNA viral
  • lipopolysaccharide bacterial
  • peptidoglycan bacterial
  • lipoarabinomannin bacterial
  • zymosan zymosan
  • mycoplasmal lipoproteins such as MALP-2 (bacterial), flagellin (bacterial) poly(inosinic-cytidylic) acid (bacterial), lipoteichoic acid (bacterial) or imi
  • Targeting molecules can be covalently bound to delivery vehicles using a variety of methods known in the art.
  • the targeting moiety is attached to the delivery vehicle by PEGylation or a biotin-avidin bridge.
  • the targeting moiety targets CD40.
  • the moiety can be a CD40 agonist.
  • the cell surface molecule CD40 is a member of the tumor necrosis factor receptor superfamily and is broadly expressed by immune, hematopoietic, vascular, epithelial, and other cells, including a wide range of tumor cells.
  • CD40 may mediate tumor regression through both an indirect effect of immune activation and a direct cytotoxic effect on the tumor, resulting in a “two-for-one” mechanism of action of CD40 agonists.
  • CD40 agonists are known in the art and reviewed in Vonderheide ( Clin Cancer Res, 13(4):1083-1088, 2007).
  • Exemplary agonists include recombinant CD40L (recombinant human trimer), CD-870, 893 (fully human IgG2 mAb), SGN-40 (humanized IgG1), and HCD 122 (fully human IgG1 mAb). Soluble agonistic CD40 antibodies have been shown to substitute for T-cell help provided by CD4+lymphocytes in murine models of T cell- mediated immunity (Khalil et al., Update Cancer Ther., 2:61-65, 2007).
  • the targeting moiety is a ligand for an integrin.
  • integrins are overexpressed on the surface of tumor cells and can thus serve as a marker that distinguishes between tumor cells and normal cells.
  • Certain integrins also activate TGF- ⁇ through an extracellular pathway. After latent TGF- ⁇ is released from a tumor cell, it binds with integrin on the surface of the tumor cell, leading to the activation of the latent TGF- ⁇ .
  • Increased TGF- ⁇ concentrations in the tumor microenvironment support immune suppression and recruit regulatory T cells to the tumor environment.
  • RGD peptide can serve a dual function: it is not only a typical integrin-targeting ligand (Ruoslahti et al., Annu. Rev. Cell Dev. Biol., 12:697-715, 1996) but also serves as an immune danger signal, activating APCs (Altincicek et al., Biol Chem., 390, 1303-11, 2009). Therefore, in a preferred embodiment, RGD peptide is loaded into, attached to the surface of, and/or enclosed within the delivery vehicle.
  • T Cell Receptor that Recognizes the p53 Antigen.
  • the targeting moiety is a T cell receptor (TCR) that recognizes the p53 antigen within the context of human MHC.
  • T cell receptor recombinant proteins derived from bacterial, eukaryotic or yeast cells include T cell receptors composed of the alpha, beta chains or gamma/delta chains ( ⁇ / ⁇ TCR or ⁇ / ⁇ TCRs).
  • IL-15/IL-15R ⁇ In another embodiment, the targeting moiety is an IL-15/IL-15R ⁇ complex.
  • Interleukin-15 IL-15
  • IL-15 is a cytokine that shares certain receptor subunits with IL-2 and thus has some overlapping mechanisms of action.
  • IL-15 is expressed by dendritic cells and provides a critical signal for the proliferation and priming of natural killer (NK) cells.
  • NK natural killer
  • Embodiments of the herein-provided immunotherapeutic constructs are agnostic as to the delivery system employed for delivery of the therapeutic agent and adjuvant.
  • the delivery system can use or be based on any type of known or to-be-developed particulate delivery vehicle.
  • nanoparticles include nanoparticles, fullerenes, endohedral metallofullerenes, trimetallic nitride templated endohedral metallofullerenes, single-walled and multi-walled carbon nanotubes, branched and dendritic carbon nanotubes, gold nanorods, silver nanorods, single-walled and multi-walled boron/nitrate nanotubes, carbon nanotube peapods, carbon nanohorns, carbon nanohorn peapods, liposomes, lipid-based nanoparticles, lipoplex, polymeric nanoparticles, calcium phosphate particles, aluminum salt particles, polyplex, nanoshells, dendrimers, microparticles, quantum dots, superparamagnetic nanoparticles, nanorods, cellulose nanoparticles, glass and polymer micro- and nano-spheres, biodegradable PLGA micro- and nano-spheres, gold nanoparticles, adjuvant particles (e.g., virosomes
  • Hybrid particles that include several classes of materials can also be used. Particles in nanometer and micron sizes can be used. The particles can be of any shape, structure, and porosity. Therapeutic agents, adjuvants, and any additional compounds can be included with the delivery agent by any suitable means, e.g., loaded into, attached to the surface of, coupled to, enclosed within, or contained within the delivery system. Such agents may be encapsulated, covalently bound, or non-covalently bound (e.g., by electrostatic, hydrophobic, van der Waals, or compound-specific interaction (such as nucleic acid base pairing, ligand-receptor, antibody-antigen, biotin-avidin, etc.)).
  • the delivery system includes a mesoporous silica nanoparticle (MSNP), such as those described in U.S. Patent Publication No. US2017/0173169 or No. US2017/0172923, the MSNPs described therein are hereby incorporated by reference.
  • MSNP mesoporous silica nanoparticle
  • the mean particle size of the mesoporous nanoparticle is about 5 nm to about 200 nm, about 5 nm to about 90 nm, about 5 nm to about 20 nm, about 30 nm to about 100 nm, about 30 nm to about 80 nm, about 30 nm to about 60 nm, about 40 nm to about 80 nm, about 70 nm to about 90 nm, or about 5 nm, about 10 nm, about 20 nm, about 30 nm, about 40 nm, about 50 nm, about 60 nm, about 70 nm, about 80 nm, about 90 nm, or about 100 nm.
  • the mesoporous silica nanoparticle is coated with cationic polymers or other compounds.
  • the cationic polymer may bind to the surface of the nanoparticle using any appropriate means. In some embodiments, the cationic polymer binds to the nanoparticle via electrostatic interaction.
  • the cationic polymer may be any polymer with a positive charge, such as, but not limited to, PEI, polyamidoamine, poly(allylamine), poly(diallyldimethylammonium chloride), chitosan, poly(N-isopropyl acrylamide-co-acrylamide), poly(N-isopropyl acrylamide-co-acrylic acid), poly(L-lysine), diethylaminoethyl-dextran, poly-(N-ethyl-vinylpyridinium bromide), poly(dimethylamino)ethyl methacrylate), or poly(ethylene glycol)-co-poly(trimethylaminoethylmethacrylate chloride).
  • PEI polyamidoamine
  • poly(allylamine) poly(diallyldimethylammonium chloride)
  • chitosan poly(N-isopropyl acrylamide-co-acrylamide), poly(N-isopropyl
  • cationic polymers will be apparent to those of skill in the art, and may be found, for example, in Polymer Handbook, 4th Edition, Edited by: Brandrup, E. H. Immergut, and E. A. Grukle; John Wiley & Sons, 2003).
  • the cationic polymers may be linear or branched. In some embodiments, the cationic polymers may range in size from about 500 Da to 25 kDa and may be branched or linear. For example, branched PEI with an average size of 1.8 kDa to 10 kDa may be loaded onto the nanoparticle core. The ratio of cationic polymer to nanoparticle may be varied depending on the desired result.
  • the cationic polymer may be present at 1 to 50 wt. % of the nanoconstruct, e.g., 5 to 40 wt. %, 10 to 30 wt. %, 20 to 30 wt. %, 5 to 15 wt. %, 5 to 20 wt. %, 5 to 25 wt.
  • the cationic polymer is present at 10 to 20 wt. %.
  • the cationic polymer is crosslinked, e.g., with a cleavable disulfide bond, pre- or post-coating on the nanoparticle.
  • the attached cationic polymer is crosslinked after binding to the nanoparticles, e.g., MSNP, using, for example, DSP (dithiobis[succinimidyl propionate]), DTSSP (3,3′-dithiobis(sulfosuccinimidyl propionate), and DTBP (dimethyl 3,3′-dithiobispropionimidate).
  • DSP dithiobis[succinimidyl propionate]
  • DTSSP dithiobis(sulfosuccinimidyl propionate)
  • DTBP dimethyl 3,3′-dithiobispropionimidate
  • a stabilizer may be conjugated to the MSNP (or a different nanoparticle) and/or the cationic polymer, e.g., by any appropriate means.
  • a stabilizer is conjugated to an amine or other reactive group of a cross-linked cationic polymer coated on the nanoparticle (e.g., a MSNP).
  • exemplary stabilizers include, but are not limited to, PEG, dextran, polysialic acid, hyaluronic acid, polyvinyl pyrrolidone, polyvinyl alcohol, and polyacrylamide, or a combination thereof.
  • a stabilizer may have multiple chemically reactive groups, e.g., for attachment to the nanoparticle, cationic polymer, and/or other component.
  • a reactive stabilizer e.g., a PEG derivative
  • the stabilizer, e.g., PEG used in conjunction with the compositions and methods of the invention generally has a molecular weight ranging between 500 Da-40 kDa, e.g., 2-10 kDa.
  • the stabilizer may be present at 1 to 50 wt.
  • % of the nanoconstruct e.g., 5 to 30 wt. %, 10 to 20 wt. %, 10 to 25 wt. %, 5 to 15 wt. %, 5 to 20 wt. %, 5 to 25 wt. %, or 1 to 10 wt. %, e.g., about 5, 10, 15, 20, 25, 35, 40 or 45 wt. %.
  • the diameter of an essentially spherical particle may refer to the physical or hydrodynamic diameter.
  • the diameter of a non-spherical particle may refer preferentially to the hydrodynamic diameter.
  • the diameter of a non-spherical particle may refer to the largest linear distance between two points on the surface of the particle.
  • Mean hydrodynamic particle size can be measured using methods known in the art, such as dynamic light scattering.
  • “Monodisperse” and “homogeneous size distribution”, are used interchangeably herein and describe a population of nanoparticles or microparticles where all of the particles are the same or nearly the same size.
  • a monodisperse distribution refers to particle distributions in which 90% of the distribution lies within 15% of the median particle size, more preferably within 10% of the median particle size, most preferably within 5% of the median particle size.
  • Nanoparticle generally refers to a particle having a diameter from 5 nm up to, but not including, 1 micron, preferably from 20 nm to 1 micron.
  • the particles can have any shape. Nanoparticles having a spherical shape are generally referred to as “nanospheres”.
  • the present disclosure is not limited to specific types or kinds of nanoparticles for complexing with adjuvants and therapeutically agents configured for treating or preventing cancer and related hyperproliferative disorders.
  • nanoparticles examples include fullerenes (a.k.a. C 60 , C 70 , C 76 , C 80 , C 84 ), endohedral metallofullerenes (EMI's), which contain additional atoms, ions, or clusters inside their fullerene cage), trimetallic nitride templated endohedral metallofullerenes (TNT EM Es, high-symmetry four-atom molecular cluster endohedrals, which are formed in a trimetallic nitride template within the carbon cage), single-walled and multi-walled carbon nanotubes, branched and dendritic carbon nanotubes, gold nanorods, silver nanorods, single-walled and multi-walled boron/nitrate nanotubes, carbon nanotube peapods (nanotubes with internal metallo-fullerenes and/or other internal chemical structures), carbon nanohorns, carbon nanohorn peapods, lipid particles liposomes, lip
  • the nanoparticle is a modified micelle.
  • the modified micelle includes polyol polymers modified to contain a hydrophobic polymer block.
  • hydrophobic polymer block indicates a segment of the polymer that on its own would be hydrophobic.
  • micelle refers to an aggregate of molecules dispersed in a liquid. A typical micelle in aqueous solution forms an aggregate with the hydrophilic “head” regions in contact with surrounding solvent, sequestering the hydrophobic single tail regions in the micelle center.
  • the head region may be, for example, a surface region of the polyol polymer while the tail region may be, for example, the hydrophobic polymer block region of the polyol polymer.
  • the invention further encompasses use of particles on the micrometer scale in addition to the nanometer scale. Where microparticles are used, it is preferred that they are relatively small, on the order of 1-50 micrometers.
  • nanoparticles encompasses true nanoparticles (sizes of from 1 nm to 1000 nm), microparticles (e.g., from 1 micrometer to 50 micrometers), or both.
  • nanoparticles include, by way of example and without limitation, paramagnetic nanoparticles, superparamagnetic nanoparticles, metal nanoparticles, fullerene-like materials, inorganic nanotubes, dendrimers, dendrimers with covalently attached metal chelates, nanofibers, nanohorns, nano-onions, nanorods, nanoropes, adjuvant particles (e.g., virosomes or other virus-like particles), and quantum dots.
  • a nanoparticle is a metal nanoparticle (for example, a nanoparticle of gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof).
  • Nanoparticles can include a core or a core and a shell, as in core-shell nanoparticles. Hybrid particles that include several classes of materials can also be used.
  • Immunotherapeutic construct-containing compositions including one or more active agents and one or more adjuvant compounds each loaded into, attached to the surface of, and/or enclosed within a delivery vehicle, are disclosed.
  • the nanoparticulate compositions offer a number of advantages over delivering the active agent or agents to the target cells in solution.
  • the nanoparticulate compositions present a localized concentration of the one or more active agents on or in a nanoparticle leading to increased avidity when the nanoparticle encounters the target cells.
  • the nanoparticulate compositions can also serve as a depot of active agent with tunable release kinetics that can extend over several days to prolong effective systemic half-life and efficacy of the agent or agents.
  • two or more active agents are loaded into, attached to the surface of, and/or enclosed within a delivery vehicle.
  • the relative concentrations of each of the two or more active agents and their location on or within the delivery vehicle can be manipulated during manufacture of the compositions to adapt a preferred dosage and presentation that will be received by the target cell.
  • Loading of two or more active agents into or onto the same delivery vehicle allows the two or more active agents to be presented to the target cell or same tumor microenvironment simultaneously or in an otherwise predetermined order to the target cell.
  • the delivery vehicles can be, for example, nanolipogels, polymeric particles, silica particles, liposomes, or multilamellar vesicles.
  • the particulate delivery vehicles are nanoscale compositions, for example, 10 nm up to, but not including, 1 micron.
  • the particles can be smaller, or larger (e.g., microparticles, etc.).
  • example immunotherapeutic constructs disclosed herein may be referred to nanoparticulate compositions, it will be appreciated that in some embodiments and for some uses the particulate compositions can be somewhat larger than nanoparticles.
  • particulate compositions can also be between 1 micron to 1000 microns. Such compositions can be referred to as microparticulate compositions.
  • the particle be of a size suitable to access the tumor microenvironment.
  • the particle is of a size suitable to access the tumor microenvironment and/or the tumor cells by enhanced permeability and retention (EPR) effect.
  • EPR refers to the property by which certain sizes of molecules (e.g., the particulate compositions discussed herein) tend to accumulate in tumor tissue much more than they do in normal tissues. Therefore, in compositions for treatment of cancer, the delivery vehicle is preferably in the range of 25 nm to 500 nm inclusive, more preferably in the range of 30 nm to 300 nm inclusive.
  • Nanolipogels are core-shell nano-particulates that combine the advantages of both liposomes and polymer-based particles for sustained delivery of active agents.
  • nanolipogels can exhibit, increased loading efficiency, increased sustained release, and improved therapeutic efficacy for combinations of macromolecules and molecules compared to conventional nanoparticle compositions.
  • the outer shell of the nanolipogel protects cargo and, provides biocompatibility as well as a surface for functionalization with targeting molecule(s).
  • the outer shell encapsulates components so they are not exposed until desired, for example, in response to environmental conditions or stimuli, creating monodisperse, reproducible particle populations, and mediating internalization into desired cell types.
  • the inner core which can be a dendrimer or other polymer, has separate and additive functionalities to the outer shell.
  • the inner shell allows for secondary deposition of drug, vaccine, or imaging agent; increases loading of components with different physiochemical properties into the particle; allows for tunable release of contents from particles; increases cytosolic availability of DNA/RNA, drug, and/or protein by disrupting endosomes, all leading to enhanced drug effects, antigen presentation, and transfection/silencing.
  • Nanolipogels have a polymer matrix core containing one or more host molecules.
  • the polymeric matrix is preferably a hydrogel, such as a crosslinked block copolymer containing one or more poly(alkylene oxide) segments, such as polyethylene glycol, and one or more aliphatic polyester segments, such as polylactic acid.
  • One or more cargo molecules is dispersed within or covalently bound to the polymeric matrix.
  • the hydrogel core is surrounded by a liposomal shell.
  • Nanolipogels can be constructed to incorporate a variety of active agents that can subsequently be released in a controlled fashion. Active agents can be dispersed within the hydrogel matrix, dispersed within the liposomal shell, covalently attached to the liposomal shell, and combinations thereof. Active agents can be selectively incorporated at each of these locales within the nanolipogel. Furthermore, the release rate of active agents from each of these locales can be independently tuned. Because each of these locales possesses distinct properties, including size and hydrophobicity/hydrophilicity, the chemical entities independently incorporated at each of these locales can differ dramatically with respect to size and composition.
  • nanolipogels can be loaded with one or more compounds dispersed within the polymeric matrix as well as small molecule hydrophobic drugs associated and adjuvant(s). Nanolipogels can be loaded provide simultaneous sustained release of agents that differ widely in chemical composition and molecular weight.
  • Nanolipogels are typically spherical in shape, with average particle sizes ranging between 50 nm and 1000 nm, more preferably between 75 nm and 300 nm, most preferably between 90 nm and 200 nm. In certain embodiments, the nanolipogels possess an average particle size between 100 nm and 140 nm. Particles may be non-spherical.
  • nanolipogels having a positive, negative, or near neutral surface charge may be prepared.
  • the nanolipogels possess a near neutral surface charge.
  • the nanolipogels possess a ⁇ -potential of between 10 mV and ⁇ 10 mV, more preferably between 5 mV and ⁇ 5 mV, more preferably between 3 mV and ⁇ 3 mV, most preferably between 2 mV and ⁇ 2 mV.
  • Hydrophobic active agents such as proteins
  • hydrophilic active agents may be covalently connected to the surface of the nanolipogel or dispersed within the liposomal shell.
  • the liposomal shell includes one or more PEGylated lipids.
  • one or more active agents may be conjugated to the terminus of one or more PEG chains present on the surface of the liposomal shell.
  • the lipid is modified to include an avidin moiety, enabling a biotinylated targeting moiety, detectable label, or other active agent to be coupled thereto, if so desired.
  • one or more active agents are covalently connected to the surface of the nanolipogel via a linking group that is cleaved in response to an external chemical or physical stimulus, such as a change in ambient pH, so as to trigger release of the active agent at a desired physiological locale.
  • the nanolipogel core is formed from a polymeric matrix.
  • the matrix can include one or more host molecules as discussed in more detail below.
  • the nanolipogel core may further include one or more active agents.
  • the active agents may be complexed to a host molecule, dispersed with polymeric matrix, or combinations thereof.
  • the polymeric matrix of the nanolipogels may be formed from one or more polymers or copolymers. By varying the composition and morphology of the polymeric matrix, one can achieve a variety of controlled release characteristics, permitting the delivery of moderate constant doses of one or more active agents over prolonged periods of time.
  • the polymeric matrix may be formed from non-biodegradable or biodegradable polymers; however, preferably, the polymeric matrix is biodegradable.
  • the polymeric matrix can be selected to degrade over a time period ranging from one day to one year, more preferably from seven days to 26 weeks, more preferably from seven days to 20 weeks, most preferably from seven days to 16 weeks.
  • Biodegradable cross-linkers may be used to increase molecular weight of polymers, which are clearable from the body as small fragments after degradation of the cross-linkers.
  • polymers are preferred, although natural polymers may be used.
  • Representative polymers include poly(hydroxy acids) such as poly(lactic acid), poly(glycolic acid), poly(lactic acid-co-glycolic acids), polyhydroxyalkanoates such as poly3-hydroxybutyrate or poly4-hydroxybutyrate; polycaprolactones; poly(orthoesters); polyanhydrides; poly(phosphazenes); poly(lactide-co-caprolactones); poly(glycolide-co-caprolactones); polycarbonates such as tyrosine polycarbonates; polyamides (including synthetic and natural polyamides), polypeptides, and poly(amino acids); polyesteramides; other biocompatible polyesters; poly(dioxanones); poly(alkylene alkylates); hydrophilic polyethers; polyurethanes; polyetheresters; polyacetals; polycyanoacrylates; polysiloxanes; poly(oxyethylene)/poly(oxypropylene)
  • “derivatives” include polymers having substitutions, additions of chemical groups and other modifications to the polymeric backbones described above routinely made by those skilled in the art.
  • Natural polymers including proteins such as albumin, collagen, gelatin, prolamines, such as zein, and polysaccharides such as alginate and pectin, may also be incorporated into the polymeric matrix. While a variety of polymers may be used to form the polymeric matrix, generally, the resulting polymeric matrix will be a hydrogel. In certain cases, when the polymeric matrix contains a natural polymer, the natural polymer is a biopolymer which degrades by hydrolysis, such as a polyhydroxyalkanoate.
  • the polymeric matrix may optionally contain one or more crosslinkable polymers.
  • the crosslinkable polymers contain one or more photo-polymerizable groups, allowing for the crosslinking of the polymeric matrix following nanolipogel formation.
  • suitable photo-polymerizable groups include vinyl groups, acrylate groups, methacrylate groups, and acrylamide groups.
  • Photo-polymerizable groups when present, may be incorporated within the backbone of the crosslinkable polymers, within one or more of the sidechains of the crosslinkable polymers, at one or more of the ends of the crosslinkable polymers, or combinations thereof.
  • the polymeric matrix may be formed from polymers having a variety of molecular weights, so as to form nanolipogels having properties, including drug release rates, optimal for specific applications.
  • the polymers which make up the polymeric matrix possess average molecular weights ranging between 500 Da and 50 kDa.
  • the polymers typically possess average molecular weights ranging between 1 kDa and 50 kDa, more preferably between 1 kDa and 70 kDa, most preferably between 5 kDa and 50 kDa.
  • the polymeric matrix is formed from crosslinkable polymers
  • the polymers typically possess lower average molecular weights ranging between 500 Da and 25 kDa, more preferably between 1 kDa and 10 kDa, most preferably between 3 kDa and 6 kDa.
  • the polymeric matrix is formed from a crosslinkable polymer having an average molecular weight of 5 kDa.
  • the polymeric matrix is formed from a poly(alkylene oxide) polymer or a block copolymer containing one or more poly(alkylene oxide) segments.
  • the poly(alkylene oxide) polymer or poly(alkylene oxide) polymer segments may contain between 8 and 500 repeat units, more preferably between 40 and 300 repeat units, most preferably between 50 and 150 repeat units.
  • Suitable poly(alkylene oxides) include polyethylene glycol (also referred to as polyethylene oxide or PEG), polypropylene 1,2-glycol, poly(propylene oxide), polypropylene 1,3-glycol, and copolymers thereof.
  • the polymeric matrix is formed from an aliphatic polyester or a block copolymer containing one or more aliphatic polyester segments.
  • the polyester or polyester segments are poly(lactic acid) (PLA), poly(glycolic acid or poly(lactide-co-glycolide) (PLGA).
  • the polymeric matrix is formed) (PGA), from a block copolymer containing one or more poly(alkylene oxide) segments, one or more aliphatic polyester segments, and optionally one or more photo-polymerizable groups.
  • the one or more poly(alkylene oxide) segments imbue the polymer with the necessary hydrophilicity, such that the resultant polymer matrix forms a suitable hydrogel, while the polyester segments provide a polymeric matrix with tunable hydrophobicity/hydrophilicity and/or the desired in vivo degradation characteristics.
  • the degradation rate of the polyester segments can be varied from days (in the case of pure PGA) to months (in the case of pure PLA), and may be readily manipulated by varying the ratio of PLA to PGA in the polyester segments.
  • the poly(alkylene oxides), such as PEG, and aliphatic polyesters, such as PGA, PLA, and PLGA have been established as safe for use in humans; these materials have been used in human clinical applications, including drug delivery systems, for more than 30 years.
  • the polymeric matrix is formed from a tri-block copolymer containing a central poly(alkylene oxide) segment, adjoining aliphatic polyester segments attached to either end of the central poly(alkylene oxide) segment, and one or more photo-polymerizable groups.
  • the central poly(alkylene oxide) segment is PEG
  • aliphatic polyesters segments are PGA, PLA, or PLGA.
  • the average molecular weight of the central poly(alkylene oxide) segment is greater than the average molecular weight of the adjoining polyester segments.
  • the average molecular weight of the central poly(alkylene oxide) segment is at least three times greater than the average molecular weight of one of the adjoining polyester segments, more preferably at least five times greater than the average molecular weight of one of the adjoining polyester segments, most preferably at least ten times greater than the average molecular weight of one of the adjoining polyester segments.
  • the central poly(alkylene oxide) segment possesses an average molecular weight ranging between 500 Da and 10,000 Da, more preferably between 1,000 Da and 7,000 Da, most preferably between 2,500 Da and 5,000 Da. In particular embodiments, average molecular weight of the central poly(alkylene oxide) segment is 4,000 Da.
  • each adjoining polyester segment possesses an average molecular weight ranging between 100 Da and 3,500 Da, more preferably between 100 Da and 1,000 Da, most preferably between 100 Da and 500 Da.
  • Examples of natural polymers include proteins such as albumin, collagen, gelatin and prolamines, for example, zein, and polysaccharides such as alginate, cellulose derivatives and polyhydroxyalkanoates, for example, polyhydroxybutyrate.
  • the in vivo stability of the microparticles can be adjusted during the production by using polymers such as poly(lactide-co-glycolide) copolymerized with polyethylene glycol (PEG). If PEG is exposed on the external surface, it may increase the time these materials circulate due to the hydrophilicity of PEG.
  • non-biodegradable polymers examples include ethylene vinyl acetate, poly(meth)acrylic acid, polyamides, copolymers and mixtures thereof.
  • the matrix can also be made of gel-type polymers, such as alginate, produced through traditional ionic gelation techniques.
  • the polymers are first dissolved in an aqueous solution, mixed with barium sulfate or some bioactive agent, and then extruded through a microdroplet forming device, which in some instances employs a flow of nitrogen gas to break off the droplet.
  • a slowly stirred (for instance, 100-170 RPM) ionic hardening bath is positioned below the extruding device to catch the forming microdroplets.
  • the microparticles are left to incubate in the bath for twenty to thirty minutes in order to allow sufficient time for gelation to occur.
  • Microparticle size is controlled by using various size extruders or varying either the nitrogen gas or polymer solution flow rates.
  • Chitosan microparticles can be prepared by dissolving the polymer in acidic solution and crosslinking it with tripolyphosphate.
  • Carboxymethyl cellulose (CMC) microparticles can be prepared by dissolving the polymer in acid solution and precipitating the microparticle with lead ions.
  • negatively charged polymers e.g., alginate, CMC
  • positively charged ligands e.g., polylysine, polyethyleneimine
  • the aliphatic polyesters specifically the hydrophobic poly(lactic acid) (PLA), more hydrophilic PGA and their copolymers, poly(lactide-co-glycolide) (PLGA).
  • PLA hydrophobic poly(lactic acid)
  • PGA poly(lactide-co-glycolide)
  • PLGA poly(lactide-co-glycolide)
  • the degradation rate of these polymers, and often the corresponding drug release rate, can vary from days (PGA) to months (PLA) and is easily manipulated by varying the ratio of PLA to PGA.
  • the physiologic compatibility of PLGA and its homopolymers PGA and PLA have been established for safe use in humans; these materials have a history of over 30 years in various human clinical applications including drug delivery systems.
  • PLGA nanoparticles can be formulated in a variety of ways that improve drug pharmacokinetics and biodistribution to target tissue by either passive or active targeting.
  • the microparticles are designed to release molecules to be encapsulated or attached over a period of days to weeks. Factors that affect the duration of release include pH of the surrounding medium (higher rate of release at pH 5 and below due to acid catalyzed hydrolysis of PLGA) and polymer composition.
  • Aliphatic polyesters differ in hydrophobicity and that in turn affects the degradation rate. Specifically the hydrophobic poly(lactic acid) (PLA), more hydrophilic PGA and their copolymers, poly(lactide-co-glycolide) (PLGA) have various release rates. The degradation rate of these polymers, and often the corresponding drug release rate, can vary from days (PGA) to months (PLA) and is easily manipulated by varying the ratio of PLA to PGA.
  • Nanolipogels include a liposomal shell composed of one or more concentric lipid monolayers or lipid bilayers.
  • the shell can further include one or more active agents, targeting molecules, or combinations thereof.
  • Nanolipogels include a liposomal shell composed of one or more concentric lipid monolayers or lipid bilayers.
  • the composition of the liposomal shell may be varied to influence the release rate of one or more active agents in vivo.
  • the lipids may also be covalently crosslinked, if desired, to alter in vivo drug release.
  • the lipid shell can be formed from a single lipid bilayer (unilamellar) or several concentric lipid bilayers (multilamellar).
  • the lipid shell may be formed from a single lipid; however, in preferred embodiments, the lipid shell is formed from a combination of more than one lipid.
  • the lipids can be neutral, anionic, or cationic at physiologic pH.
  • Suitable neutral and anionic lipids include sterols and lipids such as cholesterol, phospholipids, lysolipids, lysophospholipids, and sphingolipids.
  • Neutral and anionic lipids include phosphatidylcholine (PC) (such as egg PC, soy PC), including 1,2-diacyl-glycero-3-phosphocholines; phosphatidylserine (PS), phosphatidylglycerol, phosphatidylinositol (PI); glycolipids; sphingophospholipids, such as sphingomyelin; sphingoglycolipids (also known as 1-ceramidyl glucosides), such as ceramide galactopyranoside, gangliosides and cerebrosides; fatty acids, sterols containing a carboxylic acid group such as cholesterol or derivatives thereof; and 1,2-diacyl-sn-glycero-3-phosphoethanol
  • tissue derived L-.alpha.-phosphatidyl egg yolk, heart, brain, liver, soybean
  • synthetic e.g., saturated and unsaturated 1,2-diacyl-sn-glycero-3-phosphocholines, 1-acyl-2-acyl-sn-glycero-3-phosphocholines, 1,2-diheptanoyl-SN-glycero-3-phosphocholine
  • Suitable cationic lipids include N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethyl ammonium salts, also referred to as TAP lipids, for example as a methylsulfate salt.
  • TAP lipids include DOTAP (dioleoyl-), DMTAP (dimyristoyl-), DPTAP (dipalmitoyl-), and DSTAP (distearoyl-).
  • Suitable cationic lipids include dimethyldioctadecyl ammonium bromide (DDAB), 1,2-diacyloxy-3-trimethylammonium propanes, N-[1-(2,3-dioloyloxy)propyl]-N,N-dimethyl amine (DODAP), 1,2-diacyloxy-3-dimethylammonium propanes, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA), 1,2-dialkyloxy-3-dimethylammonium propanes, dioctadecylamidoglycylspermine (DOGS), 3-[N--(N′,N′-dimethylamino-ethane)carbamoyl]cholesterol (DC-Chol); 2,3-dioleoyloxy-N-(2-(sperminecarboxamido)-ethyl)-N,N-dimethyl
  • Suitable lipids include PEGylated derivatives of the neutral, anionic, and cationic lipids described above. Incorporation of one or more PEGylated lipid derivatives into the lipid shell can result in a nanolipogel which displays polyethylene glycol chains on its surface. The resulting nanolipogels may possess increased stability and circulation time in vivo as compared to nanolipogels lacking PEG chains on their surfaces.
  • PEGylated lipids examples include distearoylphosphatidylethanlamine-polyethylene glycol (DSPE-PEG), including DSPE PEG (2000 MW) and DSPE PEG (5000 MW), dipalmitoyl-glycero-succinate polyethylene glycol (DPGS-PEG), stearyl-polyethylene glycol and cholesteryl-polyethylene glycol.
  • DSPE-PEG distearoylphosphatidylethanlamine-polyethylene glycol
  • DPGS-PEG dipalmitoyl-glycero-succinate polyethylene glycol
  • stearyl-polyethylene glycol stearyl-polyethylene glycol
  • cholesteryl-polyethylene glycol examples include distearoylphosphatidylethanlamine-polyethylene glycol (DSPE-PEG), including DSPE PEG (2000 MW) and DSPE PEG (5000 MW), dipalmitoyl-glycer
  • the lipid shell is formed from a combination of more than one lipid. In certain embodiments the lipid shell is formed from a mixture of at least three lipids. In particular embodiments, the lipid shell is formed from a mixture of phosphatidyl choline (PC), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG), and cholesterol.
  • PC phosphatidyl choline
  • DSPE-PEG 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000]
  • the lipid shell is formed from a mixture of one or more PEGylated phospholipids and one or more additional lipids or sterols.
  • the molar ratio of the one or more PEGylated lipids to the one or more additional lipids or sterols ranges from 1:1 to 1:6, more preferably from 1:2 to 1:6, most preferably from 1:3 to 1:5.
  • the molar ratio of the one or more PEGylated lipids to the one or more additional lipids or sterols is 1:4.
  • the lipid shell is formed from a mixture of one or more phospholipids and one or more additional lipids or sterols.
  • the molar ratio of the one or more phospholipids to the one or more additional lipids or sterols ranges from 1:1 to 6:1, more preferably from 2:1 to 6:1, most preferably from 3:1 to 5:1.
  • the molar ratio of the one or more phospholipids to the one or more additional lipids or sterols is 4:1.
  • the lipid shell is formed from a mixture of a phospholipid, such as phosphatidyl choline (PC), a PEGylated phospholipid, such as 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG), and cholesterol.
  • a phospholipid such as phosphatidyl choline (PC)
  • PEGylated phospholipid such as 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (DSPE-PEG)
  • cholesterol in a 3:1:1 molar ratio.
  • the delivery vehicle can also be a polymeric particle, for example a micro- or a nanoparticle.
  • the particles can be biodegradable or non-biodegradable. Exemplary polymers that can be used to manufacture polymeric particles are discussed above with respect to the polymeric matrix component of nanolipogels.
  • biodegradable polymers examples include polymers of hydroxy acids such as lactic acid and glycolic acid, and copolymers with PEG, polyanhydrides, poly(ortho)esters, polyurethanes, poly(butyric acid), poly(valeric acid), poly(lactide-co-caprolactone), blends and copolymers thereof.
  • the particles are composed of one or more polyesters.
  • particles can contain one or more of the following polyesters: homopolymers including glycolic acid units, referred to herein as “PGA”, and lactic acid units, such as poly-L-lactic acid, poly-D-lactic acid, poly-D,L-lactic acid, poly-L-lactide, poly-D-lactide, and poly-D,L-lactide, collectively referred to herein as “PLA”, and caprolactone units, such as poly(.epsilon.-caprolactone), collectively referred to herein as “PCL”; and copolymers including lactic acid and glycolic acid units, such as various forms of poly(lactic acid-co-glycolic acid) and poly(lactide-co-glycolide) characterized by the ratio of lactic acid:glycolic acid, collectively referred to herein as “PLGA”; and polyacrylates, and derivatives thereof.
  • PGA glycolic acid units
  • PLA poly-L-lactic acid
  • PLA poly-L-lactic acid
  • PCL
  • Exemplary polymers also include copolymers of polyethylene glycol (PEG) and the aforementioned polyesters, such as various forms of PLGA-PEG or PLA-PEG copolymers, collectively referred to herein as “PEGylated polymers”.
  • PEG polyethylene glycol
  • the PEG region can be covalently associated with polymer to yield “PEGylated polymers” by a cleavable linker.
  • Alginate polymers may also be used.
  • the particles are composed of PLGA.
  • PLGA is a safe, FDA approved polymer.
  • PLGA particles are advantageous because they can protect the active agent (i.e., the encapsulant), promote prolonged release, and are amenable to the addition of targeting moieties.
  • the particles can contain one or more polymer conjugates containing end-to-end linkages between the polymer and a targeting moiety, detectable label, or other active agent.
  • a modified polymer can be a PLGA-PEG-phosphonate.
  • the particle is modified to include an avidin moiety and a biotinylated targeting moiety, detectable label, or other active agent can be coupled thereto.
  • Examples of preferred natural polymers include proteins such as albumin, collagen, gelatin and prolamines, for example, zein, and polysaccharides such as alginate, cellulose derivatives and polyhydroxyalkanoates, for example, polyhydroxybutyrate.
  • the in vivo stability of the particles can be adjusted during the production by using polymers such as poly(lactide-co-glycolide) copolymerized with polyethylene glycol (PEG). If PEG is exposed on the external surface, it may increase the time these materials circulate due to the hydrophilicity of PEG.
  • non-biodegradable polymers examples include ethylene vinyl acetate, poly(meth)acrylic acid, polyamides, copolymers and mixtures thereof.
  • Nanolipogels are a nanoparticle that combines the advantages of both liposomes and polymer-based particles for sustained delivery of nucleic acids, proteins and/or small molecules.
  • the nanolipogel can be in the form of spheres, discs, rods or other geometries with different aspect ratios.
  • the nanosphere can be larger, i.e., microparticles.
  • the nanolipogel is typically formed of synthetic or natural polymers capable of encapsulating agents by remote loading and tunable in properties so as to facilitate different rates of release. Release rates are modulated by varying the polymer to lipid ratio from 0.05 to 5.0, more preferably from 0.5 to 1.5.
  • Nanolipogels are designed to be loaded with agents either prior to, during or after formation and subsequently function as controlled-release vehicles for the agents.
  • the nanolipogel can be loaded with more than one agent such that controlled release of the multiplicity of agents is subsequently achieved.
  • the nanolipogel is loaded with one or more therapeutic agents and/or adjuvants during formation and/or following formation by the process of rehydration of the nanolipogel in the presence of the agents.
  • the nanolipogel is loaded with a molecule that serves as an adjuvant and the nanolipogel thereafter incorporates one or more anti-cancer agents after formation, for the delivery and release of adjuvant together with the anti-cancer agent(s).
  • the polymeric nanoparticle is prepared using an emulsion solvent evaporation method.
  • a polymeric material is dissolved in a water immiscible organic solvent and mixed with a drug solution or a combination of drug solutions.
  • the water immiscible organic solvent can be one or more of the following: chloroform, dichloromethane, and acyl acetate.
  • the drug can be dissolved in one or more of the following: acetone, ethanol, methanol, isopropyl alcohol, acetonitrile and dimethyl sulfoxide (DMSO).
  • DMSO dimethyl sulfoxide
  • the emulsification technique can be probe sonication or homogenization through a homogenizer.
  • the peptides or fluorophores or drugs may be associated with the surface of, encapsulated within, surrounded by, and/or distributed throughout, the polymeric matrix of the particle.
  • the polymeric nanoparticles are prepared using nanoprecipitation methods or microfluidic devices.
  • a polymeric material is mixed with a drug or drug combinations in a water miscible organic solvent.
  • the resulting mixture solution is then added to an aqueous solution to yield a nanoparticle solution.
  • Particles can be fabricated from different polymers using a variety of methods that can be selected based on criteria including the polymeric composition of the particle, the agent(s) being loaded into or associated with the particle according to method that are known in the art. Exemplary methods are provided below.
  • Solvent Evaporation In this method the polymer is dissolved in a volatile organic solvent, such as methylene chloride. The drug (either soluble or dispersed as fine particles) is added to the solution, and the mixture is suspended in an aqueous solution that contains a surface active agent such as poly(vinyl alcohol). The resulting emulsion is stirred until most of the organic solvent evaporated, leaving solid particles. The resulting particles are washed with water and dried overnight in a lyophilizer. Particles with different sizes (0.5-1000 microns) and morphologies can be obtained by this method. This method is useful for relatively stable polymers like polyesters and polystyrene.
  • labile polymers such as polyanhydrides
  • polyanhydrides may degrade during the fabrication process due to the presence of water.
  • the following two methods which are performed in completely anhydrous organic solvents, are more useful.
  • Hot Melt Microencapsulation In this method, the polymer is first melted and then mixed with the solid particles. The mixture is suspended in a non-miscible solvent (like silicon oil), and, with continuous stirring, heated to 5° C. above the melting point of the polymer. Once the emulsion is stabilized, it is cooled until the polymer particles solidify. The resulting particles are washed by decantation with petroleum ether to give a free-flowing powder. Particles with sizes between 0.5 to 1000 microns are obtained with this method. The external surfaces of spheres prepared with this technique are usually smooth and dense. This procedure is used to prepare particles made of polyesters and polyanhydrides. However, this method is limited to polymers with molecular weights between 1,000-50,000.
  • a non-miscible solvent like silicon oil
  • This technique is primarily designed for polyanhydrides.
  • the drug is dispersed or dissolved in a solution of the selected polymer in a volatile organic solvent like methylene chloride.
  • This mixture is suspended by stirring in an organic oil (such as silicon oil) to form an emulsion.
  • an organic oil such as silicon oil
  • this method can be used to make particles from polymers with high melting points and different molecular weights. Particles that range between 1-300 microns can be obtained by this procedure.
  • the external morphology of spheres produced with this technique is highly dependent on the type of polymer used.
  • the polymer is dissolved in organic solvent.
  • a known amount of the active drug is suspended (insoluble drugs) or co-dissolved (soluble drugs) in the polymer solution.
  • the solution or the dispersion is then spray-dried.
  • Hydrogel Particles Particles made of gel-type polymers, such as alginate, are produced through traditional ionic gelation techniques.
  • the polymers are first dissolved in an aqueous solution, mixed with barium sulfate or some bioactive agent, and then extruded through a microdroplet forming device, which in some instances employs a flow of nitrogen gas to break off the droplet.
  • a slowly stirred (for instance, 100-170 RPM) ionic hardening bath is positioned below the extruding device to catch the forming microdroplets.
  • the particles are left to incubate in the bath for twenty to thirty minutes in order to allow sufficient time for gelation to occur.
  • Chitosan particles can be prepared by dissolving the polymer in acidic solution and crosslinking it with tripolyphosphate.
  • Carboxymethyl cellulose (CMC) particles can be prepared by dissolving the polymer in acid solution and precipitating the particle with lead ions.
  • negatively charged polymers e.g., alginate, CMC
  • positively charged ligands e.g., polylysine, polyethyleneimine
  • the delivery vehicles are liposomes or lipid nanoparticles.
  • Liposomes are typically spherical vesicles composed of a lamellar phase lipid bilayer.
  • the liposomes can be, for example, multilamellar vesicles (MLV), small unilamellar liposome vesicles (SUV), large unilamellar vesicles (LUV), or cochleate vesicles.
  • MLV multilamellar vesicles
  • SUV small unilamellar liposome vesicles
  • LUV large unilamellar vesicles
  • cochleate vesicles cochleate vesicles.
  • Liposomes, micelles, and other lipid-based delivery vehicles useful for preparation of the disclosed nanoparticulate compositions are known in the art. See, for example, Torchilin et al. ( Adv Drug Delivery Rev, 58(14):1532-55, 2006
  • Liposomes may include N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate (DOTAP) or LipofectamineTM.
  • DOTAP N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate
  • a delivery system involving chitosan may be used as described, e.g., in Lu et al. ( Cancer Cell, 18:185-197, 2010).
  • a nanovector may be used to deliver a miRNA to a subject; nanovectors are described, e.g., in Pramanik et al. ( Mol Cancer Ther, 10:1470-1480, 2011).
  • the delivery vehicle can also be silica particles. Suitable silica particles useful for preparation of the disclosed nanoparticulate compositions are also known in the art. See, for example, Barbe et al. ( Adv Materials, 16(21):1959-1966, 2004), Ngamcherdtrakul et al. ( Adv Func Materials, 25: 2646-2659, 2015) and Argyo et al. ( Chem. Mater., 26(1):435-451, 2014).
  • a silicone nanoparticle e.g., as described in Bharali et al. PNAS, 102(32): 11539-11544, 2005
  • Solubility of silica or silicon in the body provides the ability for time-release of the agents that the particles carry.
  • biodegradable polymers or bioreducible crosslinking agents can be used to modify the silica or silicon particles to provide the time-release ability.
  • compositions for use in treating cancer, precancer, and other proliferative disease include at least two active components/agents, one of which is a therapeutically active agent that (1) causes tumor antigen release and/or (2) modulates an immunosuppressive tumor microenvironment; and another of which is an adjuvant.
  • the active agents are delivered in/associated with a delivery vehicle, such as a liposome, an organic or inorganic (nano- or micro-) particle, and so forth.
  • compositions can be provided to the cells either directly, such as by contacting it with the cell, or indirectly, such as through the action of any biological process.
  • the compositions can be formulated in a physiologically acceptable carrier or vehicle and injected into a tissue or fluid surrounding the cell.
  • the compositions can cross the cell membrane by simple diffusion, endocytosis, or by any active or passive transport mechanism.
  • a therapeutic compound When formulated in a pharmaceutical composition, a therapeutic compound (such as delivery system coupled with at least one therapeutic agent and at least one adjuvant) can be admixed with a pharmaceutically acceptable carrier or excipient.
  • a pharmaceutically acceptable carrier or excipient As used herein, the phrase “pharmaceutically acceptable” refers to molecular entities and compositions that are generally believed to be physiologically tolerable and do not typically produce an allergic or similar untoward reaction, such as gastric upset, dizziness and the like, when administered to a human or veterinary subject.
  • pharmaceutically acceptable derivative means any pharmaceutically acceptable salt, solvate or prodrug, e.g. ester, of the desired active agent, which upon administration to the recipient is capable of providing (directly or indirectly) the desired active agent, or an active metabolite or residue thereof.
  • pharmaceutically acceptable derivatives include salts, solvates, esters, carbamates, and phosphate esters.
  • compositions for therapy While it is possible to use a composition for therapy as is, it may be preferable to administer it in a pharmaceutical formulation, e.g., in admixture with a suitable pharmaceutical excipient, diluent or carrier selected with regard to the intended route of administration and standard pharmaceutical practice.
  • pharmaceutical composition or formulation includes at least one active composition, or a pharmaceutically acceptable derivative thereof, in association with a pharmaceutically acceptable excipient, diluent and/or carrier.
  • the excipient, diluent and/or carrier is “acceptable” in the sense of being compatible with the other ingredient(s) of the formulation and not significantly deleterious to the recipient thereof.
  • composition formulation disclosed herein can advantageously include any other pharmaceutically acceptable carriers which include those that do not produce significantly adverse, allergic, or other untoward reactions that outweigh the benefit of administration, whether for research, prophylactic and/or therapeutic treatments.
  • exemplary pharmaceutically acceptable excipients, diluents, and carriers for therapeutic use are well known in the pharmaceutical art, and are described, for example, in Remington: The Science and Practice of Pharmacy. Lippincott Williams & Wilkins (A. R., Gennaro edit. 2005), and in Remington's Pharmaceutical Sciences, 18th Ed. Mack Printing Company, 1990.
  • formulations can be prepared to meet sterility, pyrogenicity, general safety and purity standards as required by United States FDA Office of Biological Standards and/or other relevant foreign regulatory agencies.
  • the pharmaceutical excipient(s), diluent(s), and carrier(s) can be selected with regard to the intended route of administration and standard pharmaceutical practice.
  • Such pharmaceutical formulations may be presented for use in a conventional manner with the aid of one or more suitable excipients, diluents, and carriers.
  • Pharmaceutically acceptable excipients assist or make possible the formation of a dosage form for a bioactive material and include diluents, binding agents, lubricants, glidants, disintegrants, coloring agents, and other ingredients.
  • Preservatives, stabilizers, dyes and even flavoring agents may be provided in the pharmaceutical composition. Examples of preservatives include sodium benzoate, ascorbic acid and esters of p-hydroxybenzoic acid.
  • Antioxidants and suspending agents may be also used.
  • An excipient is pharmaceutically acceptable if, in addition to performing its desired function, it is non-toxic, well tolerated upon ingestion, and does not interfere with absorption of bioactive materials.
  • Exemplary generally used pharmaceutically acceptable carriers include any and all bulking agents or fillers, solvents or co-solvents, dispersion media, coatings, surfactants, antioxidants (e.g., ascorbic acid, methionine, vitamin E), preservatives, isotonic agents, absorption delaying agents, salts, stabilizers, buffering agents, chelating agents (e.g., EDTA), gels, binders, disintegration agents, and/or lubricants.
  • bulking agents or fillers include any and all bulking agents or fillers, solvents or co-solvents, dispersion media, coatings, surfactants, antioxidants (e.g., ascorbic acid, methionine, vitamin E), preservatives, isotonic agents, absorption delaying agents, salts, stabilizers, buffering agents, chelating agents (e.g., EDTA), gels, binders, disintegration agents, and/or lubricants.
  • antioxidants e.g
  • Exemplary buffering agents include citrate buffers, succinate buffers, tartrate buffers, fumarate buffers, gluconate buffers, oxalate buffers, lactate buffers, acetate buffers, phosphate buffers, histidine buffers and/or trimethylamine salts.
  • Exemplary preservatives include phenol, benzyl alcohol, meta-cresol, methyl paraben, propyl paraben, octadecyldimethylbenzyl ammonium chloride, benzalkonium halides, hexamethonium chloride, alkyl parabens such as methyl or propyl paraben, catechol, resorcinol, cyclohexanol and 3-pentanol.
  • Exemplary isotonic agents include polyhydric sugar alcohols including trihydric or higher sugar alcohols, such as glycerin, erythritol, arabitol, xylitol, sorbitol, or mannitol.
  • Exemplary stabilizers include organic sugars, polyhydric sugar alcohols, polyethylene glycol; sulfur-containing reducing agents, amino acids, low molecular weight polypeptides, proteins, immunoglobulins, hydrophilic polymers, or polysaccharides.
  • a “therapeutically effective amount” or “therapeutically effective dose” means the amount of a compound that, when administered to a subject for treating a state, disorder or condition, is sufficient to effect such state, disorder, or condition.
  • the “therapeutically effective amount” will vary depending on the compound, the disease and its severity and the age, weight, physical condition and responsiveness of the mammal to be treated. The exact dose and formulation will depend on the purpose of the treatment, and will be ascertainable by one skilled in the art using known techniques (see, e.g., Lieberman, Pharmaceutical Dosage Forms (vols.
  • “therapeutically effective amount” is used to mean an amount or dose sufficient to modulate, e.g., increase or decrease a desired activity e.g., by 10%, by 50%, or by 90%. Generally, a therapeutically effective amount is sufficient to cause an improvement in a clinically significant condition in the host following a therapeutic regimen involving one or more therapeutic agents.
  • concentration or amount of the active ingredient depends on the desired dosage and administration regimen, as discussed herein.
  • the actual dose amount administered to a particular subject can be determined by a physician, veterinarian, or researcher taking into account parameters such as physical, physiological and psychological factors including target, body weight, stage of cancer, the type of cancer, previous or concurrent therapeutic interventions, idiopathy of the subject, and route of administration.
  • Amounts effective for this use will depend on the severity of the disease and its location, particularly when a metastatic site is implicated, and the weight and general state of the patient being treated. Generally dosages range from 0.01 mg/kg to 100 mg/kg host body weight of immunotherapeutic construct per day, with dosages of from 0.1 mg/kg to 10 mg/kg per day being more commonly used, and for instance dosages of 3-7 mg/kg. Maintenance dosages over a prolonged period of time may be adjusted as necessary. The dosages, however, may be varied depending upon the requirements of the patient, the severity of the condition being treated, and the compound being employed. For example, dosages can be empirically determined considering the type and stage of cancer diagnosed in a particular patient.
  • the dose administered to a patient should be sufficient to effect a beneficial therapeutic response in the patient over time.
  • the size of the dose also will be determined by the existence, nature, and extent of any adverse side-effects that accompany the administration of a particular vector, or transduced cell type in a particular patient. Determination of the proper dosage for a particular situation is within the skill of the practitioner. Generally, treatment is initiated with smaller dosages which are less than the optimum dose of the compound. Thereafter, the dosage is increased by small increments until the optimum effect under circumstances is reached. For convenience, the total daily dosage may be divided and administered in portions during the day, if desired.
  • the selected dosage may be influenced by the desired therapeutic effect, the route of administration, the duration of the treatment desired, and the specific immunotherapeutic complex being employed.
  • immunotherapeutic construct can be administered in a range of 0.001 mg/kg to 100 mg/kg per administration (e.g., daily; or 2, 3, 4, 5 or more times weekly; or 2, 3, 4, 5 or more times a month, etc., as discussed in more detail below).
  • the route of administration can be a consideration in determining dosage as well.
  • a immunotherapeutic construct is administered in a range of 0.01 mg/kg to 100 mg/kg (e.g., daily; or 2, 3, 4, 5 or more times weekly; or 2, 3, 4, 5 or more times a month, etc.) by intravenous or intraperitoneal routes, or in a range of 0.0001 mg/kg to 1 mg/kg (e.g., daily; or 2, 3, 4, 5 or more times weekly; or 2, 3, 4, 5 or more times a month, etc.) for a subcutaneous route (e.g., local injection into or adjacent to a tumor or into the TME). More exemplary dosage are discussed below.
  • Suitable dosages may range from 0.01 mg/kg to 100 mg/kg of body weight per day, week, or month.
  • Exemplary doses can include 0.05 mg/kg to 10.0 mg/kg of the active compounds (immunotherapeutic constructs) disclosed herein.
  • the total daily dose can be 0.05 mg/kg to 30.0 mg/kg of an agent administered to a subject one to three times a day, including administration of total daily doses of 0.05-3.0, 0.1-3.0, 0.5-3.0, 1.0-3.0, 1.5-3.0, 2.0-3.0, 2.5-3.0, and 0.5-3.0 mg/kg/day of administration forms of a drug using 60-minute oral, intravenous or other dosing.
  • doses can be administered QD or BID to a subject with, e.g., total daily doses of 1.5 mg/kg, 3.0 mg/kg, 4.0 mg/kg, 5.0 mg/kg, or 7.5 mg/kg of a composition with up to 92-98% wt/v of the compounds disclosed herein.
  • Additional useful doses can often range from 0.1 to 5 ⁇ g/kg or from 0.5 to 1 ⁇ g/kg.
  • a dose can include 1 ⁇ g/kg, 10 ⁇ g/kg, 20 ⁇ g/kg, 40 ⁇ g/kg, 80 ⁇ g/kg, 200 ⁇ g/kg, 0.1 to 5 mg/kg or from 0.5 to 1 mg/kg.
  • a dose can include 1 mg/kg, 10 mg/kg, 20 mg/kg, 40 mg/kg, 80 mg/kg, 200 mg/kg, 400 mg/kg, 450 mg/kg, or more.
  • Therapeutic materials of the present disclosure may be employed in serious disease states, that is, life-threatening or potentially life-threatening situations. In such cases, it is possible and may be felt desirable by the treating physician to administer substantial excesses of these compositions.
  • therapeutically effective amounts can be initially estimated based on results from in vitro assays and/or animal model studies. Such information can be used to more accurately determine useful doses in subjects of interest.
  • Useful pre-clinical tests include pharmacodynamic analyses, toxicity analyses, and so forth.
  • Therapeutically effective amounts can be achieved by administering single or multiple doses during the course of a treatment regimen (e.g., hourly, every 2 hours, every 3 hours, every 4 hours, every 6 hours, every 9 hours, every 12 hours, every 18 hours, daily, every other day, every 3 days, every 4 days, every 5 days, every 6 days, weekly, every 2 weeks, every 3 weeks, or monthly).
  • a treatment regimen e.g., hourly, every 2 hours, every 3 hours, every 4 hours, every 6 hours, every 9 hours, every 12 hours, every 18 hours, daily, every other day, every 3 days, every 4 days, every 5 days, every 6 days, weekly, every 2 weeks, every 3 weeks, or monthly.
  • the effective amounts of compounds containing active agents include doses that partially or completely achieve the desired therapeutic, prophylactic, and/or biological effect.
  • the actual amount effective for a particular application depends on the condition being treated and the route of administration.
  • the effective amount for use in humans can be determined from animal models. For example, a dose for humans can be formulated to achieve local (e.g., intratumoral) or circulating levels that have been found to be effective in animals.
  • Compositions can be administered with one or more anesthetics including ethanol, bupivacaine, chloroprocaine, levobupivacaine, lidocaine, mepivacaine, procaine, ropivacaine, tetracaine, desflurane, isoflurane, ketamine, propofol, sevoflurane, codeine, fentanyl, hydromorphone, marcaine, meperidine, methadone, morphine, oxycodone, remifentanil, sufentanil, butorphanol, nalbuphine, tramadol, benzocaine, dibucaine, ethyl chloride, xylocaine, and/or phenazopyridine.
  • anesthetics including ethanol, bupivacaine, chloroprocaine, levobupivacaine, lidocaine, mepivacaine, procaine, ropivacaine, tetracaine, desflu
  • compositions disclosed herein can be used in conjunction with other cancer treatments, such as chemotherapeutic agents, radiation therapy, and/or immunotherapy.
  • the compositions described herein can be administered simultaneously with or sequentially with another treatment within a selected time window, such as within 10 minutes, 1 hour, 3 hour, 10 hour, 15 hour, 24 hour, or 48 hour time windows or when the complementary treatment is within a clinically-relevant therapeutic window.
  • compositions can be for administration by parenteral (intramuscular, intraperitoneal, intravenous (IV) or subcutaneous injection), by instillation, or in a depot, formulated in dosage forms appropriate for each route of administration.
  • parenteral intramuscular, intraperitoneal, intravenous (IV) or subcutaneous injection
  • compositions are administered systemically, for example, by intravenous or intraperitoneal administration, in an amount effective for delivery of the compositions to targeted cells.
  • Other routes include instillation or mucosal.
  • the compositions are administered locally, for example, by injection directly into a site to be treated.
  • the compositions are injected or otherwise administered directly to one or more tumors or diseased tissues.
  • local injection causes an increased localized concentration of the compositions which is greater than that which can be achieved by systemic administration.
  • the compositions are delivered locally to the appropriate cells by using a catheter or syringe. Other means of delivering such compositions locally to cells include using infusion pumps or incorporating the compositions into polymeric implants which can effect a sustained release of the compositions to the immediate area of the implant.
  • the immunotherapeutic constructs are given locally, for instance to readily accessible tumors such as melanoma, head and neck cancer, breast cancer, and lymphoma; or systemically for other cancers such as lung cancer, liver cancer, pancreatic cancer, prostate cancer, and metastatic cancers.
  • compositions described herein can be administered (on their own or as part of a combination therapy) by a variety of routes, including any convenient way for use in human or veterinary medicine.
  • a therapeutically effective amount of the desired active agent(s) can be formulated in a pharmaceutical composition to be introduced parenterally, transmucosally (e.g., orally, nasally, or rectally), or transdermally.
  • administration is parenteral, for instance, via intravenous injection, or intra-arteriole, intramuscular, intradermal, subcutaneous, intraperitoneal, intraventricular, and intracranial administration.
  • the administered may be as a bolus or by continuous infusion over a period of time, by intramuscular, intraperitoneal, intracerobrospinal, subcutaneous, intra-articular, intrasynovial, intrathecal, oral, topical, or inhalation routes.
  • implanted systems that allow local delivery to non-cutaneous tumors can also be used to deliver the herein-provided immunotherapeutic construct, e.g., hepatic artery infusion pump, convection enhanced delivery.
  • the pharmaceutical composition may be administered directly to the synovium, synovial fluid or joint capsule by injection preferably with a syringe. Administration may be local or systemic; the choice may be influenced by the condition being treated, as well as the active agent(s) and compositions being administered.
  • compositions can be made as aqueous solutions, such as in buffers such as Hanks' solution, Ringer's solution, or physiological saline.
  • the solutions can contain formulating agents such as suspending, stabilizing and/or dispersing agents.
  • the composition can be in lyophilized and/or powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.
  • compositions including an immunotherapeutic construct may be administered in an aqueous solution, by parenteral injection.
  • the injectable formulation can be in the form of a suspension or emulsion, and optionally includes pharmaceutically acceptable diluents, preservatives, solubilizers, emulsifiers, adjuvants and/or carriers.
  • Such injectable compositions can include diluents such as sterile water, buffered saline of various buffer content (e.g., Tris-HCl, acetate, phosphate), pH and ionic strength; and optionally, additives such as detergents and solubilizing agents (e.g., TWEENTM 20, TWEENTM 80 also referred to as polysorbate 20 or 80), anti-oxidants (e.g., ascorbic acid, sodium metabisulfite), and preservatives (e.g., Thimerosal, benzyl alcohol).
  • diluents such as sterile water, buffered saline of various buffer content (e.g., Tris-HCl, acetate, phosphate), pH and ionic strength
  • additives such as detergents and solubilizing agents (e.g., TWEENTM 20, TWEENTM 80 also referred to as polysorbate 20 or 80), anti-oxidants (e.g., ascorbic acid
  • non-aqueous solvents or vehicles examples include propylene glycol, polyethylene glycol, vegetable oils, such as olive oil and corn oil, gelatin, and injectable organic esters such as ethyl oleate.
  • the formulations for injection may be lyophilized and resuspended, for instance immediately before use.
  • the injectable formulation may be sterilized by, for example, filtration through a bacteria retaining filter, by incorporating sterilizing agents into the compositions, by irradiating the compositions, or by heating the compositions.
  • immunotherapeutic construct-including compositions are applied topically or by instillation.
  • Topical administration can include application to the lungs, nasal, oral (sublingual, buccal), vaginal, or rectal mucosa. These methods of administration can be made effective by formulating the shell or coating of the delivery vehicle with mucosal transport element(s).
  • Compositions can be delivered to the lungs while inhaling and traverse across the lung epithelial lining to the blood stream when delivered either as an aerosol or spray dried particles having an aerodynamic diameter of less than 5 microns.
  • a wide range of mechanical devices designed for pulmonary delivery of therapeutic products can be used, including but not limited to nebulizers, metered dose inhalers, and powder inhalers, all of which are familiar to those skilled in the art.
  • Formulations for administration to the mucosa will typically be spray dried drug particles, which may be incorporated into a tablet, gel, capsule, suspension or emulsion. Standard pharmaceutical excipients are available from any formulator.
  • Transdermal formulations may also be prepared. These will typically be ointments, lotions, sprays, or patches, all of which can be prepared using standard technology. Transdermal formulations can include penetration enhancers. Chemical enhancers and physical methods including electroporation and microneedles can work in conjunction with this method.
  • a microneedle is a micron-sized needle with a height of 10-2000 ⁇ m and a width of 10-50 ⁇ m, which can penetrate through the epidermis layer to dermal tissue directly with minimal or no pain (Hao et al., J Biomed Nanotechnol, 13(12):1581-1597, 2017).
  • metal-based or plastic microneedle rollers can be used to physically disrupt skin surface to enhance penetration of the applied topical agents (immunotherapeutic construct in this case).
  • degradable and dissolvable microneedles can contain immunotherapeutic constructs. Upon administration to skin, microneedles can dissolve and release the construct deep in layers of skin.
  • non-degradable microneedles may be coated with immunotherapeutic constructs, such that they deliver the coated construct deep in skin layers.
  • Microneedles can be fabricated from many classes of materials, including but not limited to, polymer, saccharides, polysaccharides, peptide, protein, metals, inorganic compound, and so forth (Ye et al., Adv Drug Deliv Rev, 127: 106-118, 2018). All materials and fabrication methods known in the art for microneedle technology is applicable to enhance delivery of this immunotherapeutic construct.
  • hepatic arterial infusion (HAI) pump which is an implanted chemotherapy device that delivers high concentrations of cytotoxic agents directly to liver metastases with minimal systemic toxicities (Cohen et al., The Oncologist, 8(6): 553-566, 2003), can also be utilized to deliver the herein described immunotherapeutic constructs.
  • HAI hepatic arterial infusion
  • immunotherapeutic constructs that include at least one adjuvant and at least one therapeutically active agent(s) capable of inducing antigen release and/or modulating an immunosuppressive environment (such as a tumor microenvironment)
  • an immunosuppressive environment such as a tumor microenvironment
  • hyperproliferative diseases, disorders, or conditions including cancer, symptoms of cancer, cancer progression (including from precancer to cancer), and cancer metastasis.
  • hyperproliferative diseases, disorders, or conditions include cancer.
  • the cancer may suppress the immune system of the subject or individual with the cancer.
  • the immunotherapeutic constructs as provided herein can suppress or reverse cancer-mediated immune suppression and allow for immune recognition and clearance of the malignancy.
  • treatment refers to any improvement of the cancer that occurs in a treated subject compared to an untreated subject.
  • Such an improvement can be a prevention of a worsening or progression of the cancer (e.g., improved progression-free survival).
  • such an improvement may also be a reduction or cure of the cancer or its accompanying symptoms (e.g., reduction in tumor volume, partial remission, complete remission (e.g., for 6 months, 1 year, 2 years, 3 years, 4 years, or 5 years or more), prevention of cancer recurrence or relapse, reduction of metastasis, or reduction of number of tumors or lesions).
  • a treatment may not be successful for 100% of the subjects to be treated, but successful in certain individual compared to other treatments the individual has received as determined by the person skilled in the art (e.g., physicians).
  • the term “preventing” refers to avoiding the onset of cancer as used herein or its accompanying syndromes. It will be understood that prevention refers to avoiding the onset of cancer within a certain time window in the future. Said time window shall, preferably, start upon administration of a compound in the sense of the invention and lasts for at least 1 month, at least 6 months, at least 9 months, at least 1 year, at least 2 years, at least 5 years, at least 10 years or even for the remaining physiological life span of a subject.
  • a prevention may not be successful for 100% of the subjects to be treated, but successful in certain individual compared to other treatments the individual has received as determined by the person skilled in the art (e.g., physicians). Prevention may also be in the context of a recurrence of cancer after remission, e.g., as measured by a reduction in probability for recurrence in a population. Prevention also refers to elimination of cancer cells anywhere in the body that could otherwise regrow and exhibit positive prognosis and disease symptoms at later dates.
  • compositions can be used to treat benign or malignant cancers, and tumors thereof.
  • the treatment can directly target and kill cancer cells, indirectly target the cancer cells by increasing an immune response against the cancer cells; or a combination thereof.
  • malignant tumors exhibit metastasis.
  • small clusters of cancerous cells dislodge from a tumor, invade the blood or lymphatic vessels, and are carried to other tissues, where they continue to proliferate. In this way a primary tumor at one site can give rise to a secondary tumor at another site.
  • compositions can delay or inhibit the growth of a tumor in a subject, reduce the growth or size of the tumor or eliminate it altogether, inhibit or reduce metastasis of the tumor, and/or inhibit or reduce symptoms associated with tumor development or growth.
  • the compositions reduce tumor burden in the subject or slow or prevent tumor growth over time.
  • Malignant tumors may be classified according to the embryonic origin of the tissue from which the tumor is derived.
  • Carcinomas are tumors arising from endodermal or ectodermal tissues such as skin or the epithelial lining of internal organs and glands.
  • Sarcomas which arise less frequently, are derived from mesodermal connective tissues such as bone, fat, and cartilage.
  • the leukemias and lymphomas are malignant tumors of hematopoietic cells of the bone marrow. Leukemias proliferate as single cells, whereas lymphomas tend to grow as tumor masses. Malignant tumors may show up at numerous organs or tissues of the body to establish a cancer.
  • the types of cancer that can be treated with the provided compositions and methods include, but are not limited to, vascular cancers such as multiple myeloma, as well as solid cancers, including adenocarcinomas and sarcomas, of bone, bladder, brain, breast, cervix, colon, rectum, esophagus, kidney, liver, lung, nasopharynx, pancreas, prostate, skin, stomach, and uterus.
  • the disclosed compositions are used to treat multiple cancer types concurrently.
  • the compositions can also be used to treat metastases or tumors at multiple locations.
  • Administration is not limited to the treatment of an existing tumor but can also be used to prevent or lower the risk of developing such diseases in an individual, i.e., for prophylactic use and to reduce spread of cancer, for instance through metastasis.
  • Potential candidates for prophylactic vaccination include individuals with a high risk of developing cancer, i.e., with a personal or familial history of certain types of cancer.
  • an immunotherapeutic construct including an adjuvant agent CpG, siRNA against BRAFV600E to kill melanoma and precursor cells (melanocytes in moles) leading to antigen release, and siRNA against STAT3 to mitigate the immunosuppressive environment, can be used to create adaptive immunity in subjects with high-risk of development melanoma and melanoma patients.
  • the immunotherapeutic construct will not only prevent and treat melanoma, but will also provide the protection from future recurrence or relapse after surgery for later stage melanoma patients.
  • siRNA against other genes along with targeting agents may be incorporated on or in the nanoparticle/construct.
  • immunotherapeutic constructs e.g., containing CpG or another adjuvant, along with a chemodrug, a targeted therapy, and/or siRNA against STAT3
  • cancer refers to all types of cancer, neoplasm or malignant tumors found in mammals, including leukemia, lymphoma, carcinomas and sarcomas.
  • Exemplary cancers that may be treated with a compound, pharmaceutical composition, or method provided herein include lymphoma, sarcoma, bladder cancer, bone cancer, brain tumor, cervical cancer, colon cancer, esophageal cancer, gastric cancer, head and neck cancer, kidney cancer, myeloma, thyroid cancer, leukemia, prostate cancer, breast cancer (e.g., triple negative, ER positive, ER negative, chemotherapy resistant, Herceptin® resistant, HER2 positive, doxorubicin resistant, tamoxifen resistant, ductal carcinoma, lobular carcinoma, primary, metastatic), ovarian cancer, pancreatic cancer, liver cancer (e.g.
  • lung cancer e.g. non-small cell lung carcinoma, squamous cell lung carcinoma, adenocarcinoma, large cell lung carcinoma, small cell lung carcinoma, carcinoid, sarcoma), glioblastoma multiforme, glioma, melanoma, prostate cancer, castration-resistant prostate cancer, breast cancer, triple negative breast cancer, glioblastoma, ovarian cancer, lung cancer, squamous cell carcinoma (e.g. head, neck, or esophagus), colorectal cancer, leukemia, acute myeloid leukemia, lymphoma, B cell lymphoma, or multiple myeloma.
  • lung cancer e.g. non-small cell lung carcinoma, squamous cell lung carcinoma, adenocarcinoma, large cell lung carcinoma, small cell lung carcinoma, carcinoid, sarcoma
  • glioblastoma multiforme glioma, melanoma
  • prostate cancer
  • Additional examples include, cancer of the thyroid, endocrine system, brain, breast, cervix, colon, head & neck, esophagus, liver, kidney, lung, non-small cell lung, melanoma, mesothelioma, ovary, sarcoma, stomach, uterus or Medulloblastoma, Hodgkin's Disease, Non-Hodgkin's Lymphoma, multiple myeloma, neuroblastoma, glioma, glioblastoma multiforme, ovarian cancer, rhabdomyosarcoma, primary thrombocytosis, primary macroglobulinemia, primary brain tumors, cancer, malignant pancreatic insulinoma, malignant carcinoid, urinary bladder cancer, premalignant skin lesions, testicular cancer, lymphomas, thyroid cancer, neuroblastoma, esophageal cancer, genitourinary tract cancer, malignant hypercalcemia, endometrial cancer
  • cancer refers to a condition or growth that precedes or develops into a cancer.
  • cancer metastasis refers to the spread of cancer cells or a tumor from one organ or part of the body to another organ or part of the body.
  • leukemia refers broadly to progressive, malignant diseases of the blood-forming organs and is generally characterized by a distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemia is generally clinically classified on the basis of (1) the duration and character of the disease-acute or chronic; (2) the type of cell involved; myeloid (myelogenous), lymphoid (lymphogenous), or monocytic; and (3) the increase or non-increase in the number abnormal cells in the blood-leukemic or aleukemic (subleukemic).
  • Exemplary leukemias that may be treated with a compound, pharmaceutical composition, or method provided herein include, for example, acute nonlymphocytic leukemia, chronic lymphocytic leukemia, acute granulocytic leukemia, chronic granulocytic leukemia, acute promyelocytic leukemia, adult T-cell leukemia, aleukemic leukemia, a leukocythemic leukemia, basophilic leukemia, blast cell leukemia, bovine leukemia, chronic myelocytic leukemia, leukemia cutis, embryonal leukemia, eosinophilic leukemia, Gross' leukemia, hairy-cell leukemia, hemoblastic leukemia, hemocytoblastic leukemia, histiocytic leukemia, stem cell leukemia, acute monocytic leukemia, leukopenic leukemia, lymphatic leukemia, lymphoblastic leukemia, lymphocytic leukemia, lymphogenous le
  • sarcoma generally refers to a tumor which is made up of a substance like the embryonic connective tissue and is generally composed of closely packed cells embedded in a fibrillar or homogeneous substance.
  • Sarcomas that may be treated with a compound, pharmaceutical composition, or method provided herein include a chondrosarcoma, fibrosarcoma, lymphosarcoma, melanosarcoma, myxosarcoma, osteosarcoma, Abemethy's sarcoma, adipose sarcoma, liposarcoma, alveolar soft part sarcoma, ameloblastic sarcoma, botryoid sarcoma, chloroma sarcoma, chorio carcinoma, embryonal sarcoma, Wilms' tumor sarcoma, endometrial sarcoma, stromal sarcoma, Ewing's sarcoma, fascial sar
  • melanoma is taken to mean a tumor arising from the melanocytic system of the skin and other organs.
  • Melanomas that may be treated with a compound, pharmaceutical composition, or method provided herein include, for example, acral-lentiginous melanoma, amelanotic melanoma, benign juvenile melanoma, Cloudman's melanoma, S91 melanoma, Harding-Passey melanoma, juvenile melanoma, lentigo maligna melanoma, malignant melanoma, nodular melanoma, subungal melanoma, or superficial spreading melanoma.
  • carcinoma refers to a malignant new growth made up of epithelial cells tending to infiltrate the surrounding tissues and give rise to metastases.
  • exemplary carcinomas that may be treated with a compound, pharmaceutical composition, or method provided herein include, for example, medullary thyroid carcinoma, familial medullary thyroid carcinoma, acinar carcinoma, acinous carcinoma, adenocystic carcinoma, adenoid cystic carcinoma, carcinoma adenomatosum, carcinoma of adrenal cortex, alveolar carcinoma, alveolar cell carcinoma, basal cell carcinoma, carcinoma basocellulare, basaloid carcinoma, basosquamous cell carcinoma, bronchioalveolar carcinoma, bronchiolar carcinoma, bronchogenic carcinoma, cerebriform carcinoma, cholangiocellular carcinoma, chorionic carcinoma, colloid carcinoma, comedo carcinoma, corpus carcinoma, cribriform carcinoma, carcinoma en cuirasse, carcinoma cutaneum, cylindrical carcinoma, cylindrical cell carcinoma, duct carcinoma, ductal carcinoma, carcinoma durum, embryonal carcinoma
  • Kits can include one or more containers including (containing) one or more or more compounds or complexes (e.g., anti-cancer agents) as described herein, optionally along with one or more additional agents for use in therapy. For instance, some kits will include an amount of at least one additional anti-cancer composition, or an amount of at least one additional anti-inflammatory agent, or both.
  • compounds or complexes e.g., anti-cancer agents
  • any active component in a kit may be provided in premeasured dosages, though this is not required; and it is anticipated that certain kits will include more than one dose.
  • Kits can also include a notice in the form prescribed by a governmental agency regulating the manufacture, use, or sale of pharmaceuticals or biological products, which notice reflects approval by the agency of manufacture, use, or sale for human administration.
  • the notice may state that the provided active ingredients can be administered to a subject.
  • the kits can include further instructions for using the kit, for example, instructions regarding administration; proper disposal of related waste; and the like.
  • the instructions can be in the form of printed instructions provided within the kit or the instructions can be printed on a portion of the kit itself. Instructions may be in the form of a sheet, pamphlet, brochure, CD-ROM, or computer-readable device, or can provide directions to instructions at a remote location, such as a website.
  • kits can also include some or all of the necessary medical supplies needed to use the kit effectively, such as applicators, ampules, sponges, sterile adhesive strips, Chloraprep, gloves, and the like. Variations in contents of any of the kits described herein can be made.
  • the instructions of the kit will direct use of the active ingredient(s) included in that kit to effectuate a clinical and/or therapeutic use described herein.
  • Example 1 Utilizing Nanotechnology to Engineer the Tumor into a Depot for Cancer Vaccination that Primes Systemic Antitumor Immunity
  • Immune checkpoint inhibitors such as inhibitors for PD-L1/PD-1, CTLA-4, etc.
  • ICIs have shown impressive outcome in clinics (Sharon et al., Chin J Cancer, 33(9):434-44, 2014; Buchbinder & Desai, Am J Clin Oncol, 39(1):98-106, 2016).
  • Immune checkpoint inhibitors release the brake of patients' own immune system to fight cancer, offering immunological memory and resulting in long-lasting immune response even after treatment stops.
  • ICIs can thus provide more durable response in late-stage cancer than chemotherapy and targeted therapy can, as shown in FIG. 1 .
  • In situ tumor vaccination is a strategy in which tumors are locally killed, releasing tumor antigens in the presence of immunostimulation, which together prime systemic adaptive immunity against tumors (Pierce et al., Hum Vaccin Immunother, 11(8):1901-9, 2015).
  • This strategy has great promise because it circumvents the need to pre-identify tumor antigens in conventional cancer vaccine development.
  • This is also a personalized therapy, since a unique set of tumor antigens is released and primes specific immunity for each patient.
  • the herein provided immunotherapeutic approach for cancer utilizes patients' own tumors as a depot for a personalized set of tumor antigens (in situ tumor vaccination).
  • AIRISE Algmenting Immune Response and Inhibiting Suppressive Environment of Tumors
  • AIRISE is aimed to improve patient survival outcomes when used alone or together with checkpoint inhibitors ( FIG. 1 ).
  • the provided particles carry adjuvant (e.g., CpG) and one or more compounds (siRNA, drug, small molecules, etc.) that cause antigen release and/or modulate immunosuppressive tumor microenvironment (e.g., docetaxel, siRNA against STAT3).
  • tumor antigens Upon treatment with a provided immunotherapeutic construct at a tumor site (for instance, through local intratumoral injection or tumor homing via systemic delivery), tumor antigens are released in the presence of immunostimulation (provided by the adjuvant), initiating adaptive immunity. Simultaneously, a compound that modulates the immunosuppressive tumor microenvironment can be co-delivered on the same nanoparticle, maximizing in situ tumor vaccination effect.
  • Tumor antigens can be taken up by AIRISE-activated antigen-presenting cells (APCs), which present the antigen to na ⁇ ve T cells. T cells (against those tumor antigens) are primed and activated into effector T cells (either in lymph nodes or in tumor site) and proliferate throughout the body.
  • APCs AIRISE-activated antigen-presenting cells
  • Effector T cells will home specifically to tumors sharing the same tumor antigens wherever they are located in the body (e.g., both locally treated tumor and untreated metastatic tumors elsewhere in the body). Death of cancer cells by cytotoxic T cells releases more tumor antigens, amplifying the process of anti-tumor T cell generation in a positive feedback loop. Notably, even when the treatment is local (e.g., intratumoral injection), the vaccination effect induced locally at the tumor site generates systemic long-lasting anti-tumor immune response ( FIG. 2 ).
  • AIRISE can be injected directly into melanoma lesions, and the treatment may affect both the injected tumor and untreated metastasized melanoma tumors in lung or liver.
  • the cargo combination can be applied on any type of micro/nanoparticles to make AIRISE.
  • Examples that follow utilize established mesoporous silica nanoparticles (U.S. Patent Application Publication 2017/0172923) as a proof of concept.
  • Another examples shown in FIG. 11 utilized cationic lipid particles with the hydrodynamic size of 1.1 micron for in situ vaccination with CpG and siSTAT3, and yield similar outcome with FIG. 7 , suggesting that various types and sizes of particles can be used.
  • Nanoparticle synthesis and characterization Mesoporous silica-based nanoparticles were synthesized as previously described (Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015; Ngamcherdtrakul et al., International J Nanomed, 13:4015-4027, 2018). Briefly, mesoporous silica nanoparticle (MSNP) was synthesized by sol-gel synthesis. The MSNP core was coated layer-by-layer with polyethylenimine (PEI) and polyethyleneglycol (PEG). PEI on the MSNP was also cross-linked for enhanced oligonucleotide delivery efficacy and safety as explained previously (Ngamcherdtrakul et al. Advanced Functional Materials, 25(18):2646-2659, 2015). MSNP coated with crosslinked PEI and PEG is referred to henceforth as “NP” in this Example.
  • PEI polyethylenimine
  • PEG polyethyleneglycol
  • siRNA and CpG were loaded electrostatically on the nanoparticle (NP) by 10-minute mixing, though a shorter time (2-5 minutes) was also effective.
  • Mitoxantrone was also loaded to the NP by 4-h mixing at room temperature in aqueous solution (e.g., PBS). Loading was performed in a complete binding manner as confirmed by the absence of free cargo molecules in the supernatant upon separating out cargo-loaded NP by centrifugation. The cargo content was measured by spectrophotometry.
  • siRNA was conjugated with Dy677 dye (Dharmacon) and thus was quantified by fluorescence signal.
  • CpG was measured with Nanodrop Spectrophotometry.
  • Unbound siRNA and CpG could also be measured by gel electrophoresis. Mitoxantrone (MTX) was quantified by absorbance measurement at 658 nm. Final NP with CpG and siRNA was characterized for hydrodynamic size in PBS by Zetasizer ( FIGS. 26 and 29 ).
  • MTX Mitoxantrone
  • Docetaxel was loaded in excess on the nanoparticle before PEI binding. Briefly, MSNP was mixed with docetaxel in ethanol overnight before PEI binding. Unbound docetaxel and PEI were washed out in PBS. PEI-NP(DTX) was then conjugated with PEG following previous methods (Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015; Ngamcherdtrakul et al., International J Nanomed, 13:4015-4027, 2018). The final products contain 0.5-1.7 wt. % DTX at the starting DTX to MSNP mass ratio of 0.2-0.8. They have the DLS size of 100 nm.
  • a cocktail of PD-1 Ab (200 ⁇ g/mouse) and CTLA-4 Ab (100 ⁇ g/mouse) was given intraperitoneally on the same day (3 doses every 3 days) as intratumoral treatment of the NP compounds.
  • mice treated with test compound/construct was injected intraperitoneally with CD8 antibody (200 ⁇ g/mouse), starting one day before first intratumoral treatment and continuing throughout the entire study.
  • Flow cytometry was typically performed in two separate antibody panels (lymphoid and myeloid) by BD Fortessa (4 lasers, up to 18 parameters). Fluorescence compensation was performed to ensure robustness of multi-color flow cytometry analysis following the established protocol known in the art.
  • AIRISE uptake study in tumor microenvironment FIG. 18
  • AIRISE-02 loaded with Alexa488 dye conjugated siSCR instead of siSTAT3
  • tumors treated and untreated
  • the presence of AIRISE-02 in different cell populations in the tumor was analyzed by flow cytometry.
  • LLC-JSP metastatic murine lung tumor model LLC-JSP (200,000 cells) were injected intravenously (tail-vein) to 6 week old C57BU6 mice. Three days after cancer cell injection, mice were randomly grouped and treated with the test compounds/constructs every 3 days for a total of 4 doses. In this model, AIRISE-02 was injected intravenously through the tail-vein instead of intratumorally.
  • CT26 bilateral ectopic tumor models. 250K and 100K CT26 (murine colorectal cancer) cells were implanted into bilateral abdomens of each mouse (Balb/c). 15 days after tumor implantation, mice were treated with the test compounds/constructs.
  • mice 4T1 bilateral orthotopic tumor models. 100K and 40K 4T1 cells were implanted into bilateral mammary fat pads of each mouse (Balb/c). 8 or 11 days after tumor implantation (as specified), mice were treated with the test compounds/constructs.
  • DLN local draining lymph nodes
  • NDLN non-draining lymph nodes
  • CpG-loaded nanoparticles activated dendritic cells in local DLN significantly better than free CpG ( FIG. 3 ).
  • CpG-nanoparticles also have added potential to co-deliver several therapeutic cargos to the same site.
  • the exemplary nanoparticle was highly optimized for delivery of siRNA, which can modulate immunosuppressive characteristics of tumor at the mRNA level.
  • Co-delivering CpG (or another adjuvant) with siRNA or other targeted molecules that tackle several hallmarks of tumor immunosuppression is proposed to beneficially prime immunotherapeutic effects.
  • Adjuvant-Loaded Nanoparticles Triggers In situ Tumor Vaccination
  • mice bearing two (bilateral) melanoma tumors were used.
  • the treatment was injected intratumorally to only one of the two tumors, while the other tumor was left untreated. Growth of both tumors was monitored; the intratumoral treatment of CpG-NP successfully primed a whole-body immune response and induced a strong abscopal effect—inhibition of both locally treated and distant untreated tumors ( FIG. 4 ). As a result, survival is also prolonged.
  • the herein described immunotherapeutic construct technology triggers in situ vaccination, thus there is no need for tumor antigens to be loaded onto or into any of the complexes.
  • Intratumoral injection of chemotherapy drugs to trigger in situ tumor vaccination has not been widely explored.
  • intratumoral injection of nanoparticle containing chemotherapy drug and adjuvant has not been done before. This is due to potential toxicity of chemotherapy drug to immune cells that may negate any activated immunotherapeutic response.
  • CpG-DTX-NP chemotherapeutic drug docetaxel
  • CpG-DTX-NP can control local treated tumor better than CpG-NP.
  • CpG-DTX-NP still induces in situ vaccination effect slightly better than CpG-NP, as shown by the control of the distant tumor and prolonged survival of the mice.
  • DTX-NP does not show any significant activity.
  • FIG. 5A T cell status was characterized in the local and distant tumors at day 7 post first dose.
  • CpG-DTX-NP shortened as “NP” in FIG. 6
  • NP cytotoxic CD8+ T cells
  • FIG. 6E which were also more activated ( FIG. 6F ).
  • the activated CD8+ T cells were more proliferative (by Ki67 marker) in the DLN of both tumors ( FIG. 6G ). More proliferating and activated CD8+ T cells were observed in non-DLN ( FIG. 6H ), suggesting that T cells were transported outside the local lymph node (e.g., in blood), contributing to the abscopal effect of the treatment.
  • FIG. 6H More proliferating and activated CD8+ T cells were observed in non-DLN ( FIG. 6H ), suggesting that T cells were transported outside the local lymph node (e.g., in blood), contributing to the abscopal effect of the treatment.
  • These T cell characteristics indicate that the herein-described nanoparticles can increase the beneficial antitumor T cell repertoires (non-exhausted state), generating whole-body immunity. While the DTX on our NP killed tumor cells to release tumor antigens, it did not harm CD8+ T cells,
  • siSTAT3 (2 wt %) was loaded in the mesoporous silica core of NP, and CpG oligo (10 wt. %) on the external surface (bound to cationic polymer layer but protected under PEG from enzymatic degradation). It is proposed that tumor antigens (already in the tumor or released by cancer death upon treatment) will prime anti-tumor immunity in the presence of immunostimulation by CpG.
  • siSTAT3 modulates immunosuppressive tumor environment, amplifying immunotherapeutic response.
  • siSTAT3-CpG-NP (AIRISE-02) was evaluated in a bilateral B16F10 melanoma tumor model in mice ( FIG. 7A ).
  • FIGS. 7B -7C also shows that siSTAT3-CpG-NP is superior to CpG-NP and siSTAT3-NP.
  • mice were treated with AIRISE-02 (siSTAT3-CpG-NP) in the same manner as FIG. 7 .
  • Mice were sacrificed one day after the third dose (or 7 days after the first dose).
  • Tumors and associated draining lymph nodes (DLNs) were collected and subjected to immune profiling with multi-color flow cytometry.
  • FIG. 17 shows that AIRISE-02 resulted in significantly higher CD8/Treg ratios in both local (treated) and distant (untreated) tumors and associated DLNs (p ⁇ 0.05 for AIRISE-02 vs. saline), confirming successful in situ tumor vaccination.
  • Regulatory T cells are typically elevated in patients' tumors and suppress anti-tumor immune response, including CD8+ T cell activity. Thus, higher intratumoral CD8/Treg ratio is desirable and is one indicator of prolonged survival in cancer patients.
  • CpG-NP did not significantly increase CD8/Treg in either of the tumors or lymph nodes at this timepoint, corresponding with its poorer efficacy than AIRISE-02 in FIG. 7 .
  • the effector CD8+ T cells in the lymph nodes were more proliferative (Ki-67) in AIRISE-02-treated mice than other control groups ( FIG. 17C ).
  • mice were treated (similar model to FIG. 7 ) by intratumorally injecting siRNA-CpG-NPs (AIRISE-02).
  • siRNA was tagged with Alexa-488.
  • FIG. 18 shows that at two hours after intratumoral injection, siRNA-CpG-NPs were taken up by 15% of cells in the TME, while CpG-siSTAT3 conjugates in prior work were reported to be taken up by only 2% of cells in the TME at 1 and 3 hours after intratumoral injection (Kortylewski et al., Nature biotechnology, 27(10):925-932, 2009).
  • NP treatment since NP treatment generates more CD8+ T cell repertoires, it is proposed that this treatment can be beneficially combined with checkpoint inhibitor treatment to enhance anti-cancer effects.
  • NP treatment (intratumorally into only one of the two tumors) has similar efficacy to the checkpoint inhibitor cocktail (given intraperitoneally) in terms of survival ( FIG. 9D ).
  • the combination of AIRISE-02 and checkpoint inhibitor cocktails substantially improved the efficacy in terms of controlling local ( FIG. 9B ) and distant tumors ( FIG.
  • FIG. 10A shows the treatment schedule of AIRISE-02 via tail vein in mice bearing Lewis Lung Carcinoma (LLC-JSP) tumors. Prolonged survival was observed ( FIG. 10B ), suggesting successful anti-cancer immunotherapy effect.
  • LLC-JSP Lewis Lung Carcinoma
  • FIGS. 11A-11C show that a very similar response was achieved with cationic lipid as had been observed with mesoporous silica nanoparticles ( FIG. 7 ). This illustrates that, for intratumoral injection, diverse types of nanoparticles can be used to create the therapeutics with the cargo combinations described herein.
  • NP can deliver siRNA along with CpG to both cancer and immune cells, resulting in knockdown of the target gene.
  • NP can deliver siRNA to both cancer and immune cells and knock down STAT3 gene (as an example) as shown in FIG. 12A for B16F10 cancer cells, FIG. 12B for J774 macrophage, and FIG. 12C for mouse primary DCs.
  • STAT3 gene as shown in FIG. 12A for B16F10 cancer cells, FIG. 12B for J774 macrophage, and FIG. 12C for mouse primary DCs.
  • siSCR-NP also decreased STAT3 level (see FIG. 12C vs. untreated) in DCs. This was not caused by nanoparticle toxicity since the cell viability was unchanged versus untreated control ( FIG. 34 ), and STAT3 mRNA was normalized with housekeeping mRNA.
  • FIG. 19 shows that STAT3 is so conserved that the same siSTAT3 sequence can knock down STAT3 in canine, murine, and human cells, facilitating direct translation from murine studies to dog and human studies. Hence, the same siSTAT3 sequence was used throughout the application across species.
  • ex vivo engineered immune cells can be administered back to patients for therapeutic effect and immune response (e.g., to kill cancer cells).
  • the cells can be derived from the treated patients or from different healthy donors (e.g., stem cells and their derivatives—Senju et al., Int J Hematol, 91(3):392-400, 2010).
  • Cancer cells may be treated with our immunotherapeutic construct ex vivo (with or without additional agents) to create a whole cell cancer vaccine (Keenan et al., Int J Hematol, 91(3):392-400, 2010; Goldstein et al., Int J Hematol, 117:118-127, 2011).
  • AIRISE-02 (siSTAT3-CpG-NP) has efficacy in other tumor models, including colon cancer ( FIG. 20 ) and breast cancer ( FIGS. 21 &22).
  • ICIs given systemically i.p.
  • AIRISE given locally into one of the two tumors in each mouse provides better efficacy than either AIRISE alone or ICIs alone.
  • test article-related effects on clinical observations In term of dermal observations, for low dose, there was no article-related dermal observations; for medium dose, test article-related edema (grades 2-3) was noted at the 48 and/or 72-hour post-dosing, but resolved 7 days after; for high dose, test article-related edema (grades 1-3) and erythema (grade 1) were noted in all animals beginning as early as 24-hour post-dosing and continuing through 7 days (the last observation) but not of concern. There were no test article-related effects on body weight. There were no test article-related effects on hematology parameters. There were no test article-related effects on coagulation parameters. There were no test article-related effects on clinical chemistry parameters.
  • Example 2 Immunotherapeutic Constructs (such as NPs) can also be Loaded with Two Types of siRNAs Simultaneously Without Losing Efficacy
  • the herein-described immunotherapeutic constructs can also co-deliver multiple siRNAs.
  • FIG. 13 shows that when NP (prepared essentially as described in Example 1) was loaded with two individual siRNA against HER2 or STAT3, specific knockdown of each protein was achieved.
  • FIG. 13 also shows that when both siRNAs were loaded on the same NP vial (see T-siHER2/siSTAT3-NP), a similar knockdown of the two proteins was achieved as when they were loaded on two separate NP vials (see T-siHER2-NP+T-siSTAT3-NP).
  • FIG. 36 shows the effectiveness of NP loaded with siCXCR4, siSTAT3, and CpG (siSTAT3/siCXCR4-CpG-NP) in enhancing the efficacy of immune checkpoint inhibitors in melanoma model.
  • Nanoparticle delivery as described herein is also beneficial because it allows loading of more than one siRNA that each can kill cancer cells and/or modulate multiple aspects of immunosuppression.
  • NP can deliver an siRNA or a cocktail of two or more different siRNAs, wherein siRNAs may: provide cytotoxic effect to cancer cells, negate immunosuppressive tumor microenvironment, contain immunostimulatory sequences, or have any combination of these characteristics.
  • the immunotherapeutic constructs can tackle different immunosuppressive pathways or different immune cell population by utilizing antibodies (or other targeting agents) on our nanoparticles for specific delivery.
  • Immunotherapeutic constructs including the NPs provided herein, can be conjugated with targeting agent(s) for targeted-delivery to specific cell population(s), such as specific cells in a tumor.
  • targeting agent(s) for targeted-delivery to specific cell population(s), such as specific cells in a tumor.
  • Some antibodies such as anti-PD-L1 antibody may serve as both targeting agents and modulators of immunosuppressive pathways.
  • FIGS. 14A-14C provide examples of such targeting of NPs.
  • NP conjugated with cetuximab show preferential uptake to EGFR+ cells over low EGFR cells ( FIGS. 14A-14B ).
  • NP conjugated with trastuzumab shows preferential uptake to HER2+ cells over low HER2 cells ( FIG. 14C ) (Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015).
  • PD-L1 antibody (mouse PD-L1 from BioXcell) is conjugated on mesoporous silica nanoparticles prepared essentially as in Example 1, which is loaded with a PLK1 inhibitor (volasertib) and CpG.
  • the PD-L1 antibody serves as both an ICI and a tumor-homing agent (targeting agent).
  • Volasertib (PLK1 inhibitor; iPLK1) was loaded in excess on the nanoparticle before PEI binding. Briefly, MSNP was mixed with volasertib in ethanol/DMSO solution overnight before PEI binding. Unbound docetaxel or volasertib and PEI were washed out in PBS. PEI-NP(iPLK1) was then conjugated with PEG following previously published methods (Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015; Ngamcherdtrakul et al., International J Nanomed, 13:4015-4027, 2018). The final products contain 0.5-2 wt. % iPLK1. They have the DLS size of 100 nm.
  • PD-L1 antibody was thiolated and conjugated to the end of PEG layer on the nanoparticle following our previously published methods (Ngamcherdtrakul et al., Advanced Functional Materials, 25(18):2646-2659, 2015; Ngamcherdtrakul et al., International J Nanomed, 13:4015-4027, 2018).
  • mice received intratumoral treatments of saline, PD-L1 antibody coated nanoparticle (p-NP), nanoparticle loaded with PLK1 inhibitor (iPLK1-NP), p-NP loaded with PLK1 inhibitor (p-iPLK1-NP), or p-NP loaded with PLK1 inhibitor and CpG (p-iPLK1-NP-CpG) to the left (local) tumor (while the distant tumor was left untreated).
  • p-NP 0.5 mg NP (2.5 ⁇ g iPLK1, 20 ⁇ g PD-L1 antibody, 20 ⁇ g CpG) in 50 ⁇ l was administered every 3 days for a total of 3 doses.
  • the immunotherapeutic construct prolonged survival of the mice over the same immunotherapeutic construct but without CpG ( FIG. 15 ).
  • the immunotherapeutic construct was also much more effective than the free PD-L1 antibody and volasertib each given at 5-fold higher concentration of what on the nanoparticle.
  • Incorporating the adjuvant (CpG) on the same NP improved the survival further. For instance, we found that incorporation of CpG on p-iPLK1-NP (referred to as p-iPLK1-NP-CpG) significantly improved survival of 2 out of 7 mice, and one mouse was completely free of tumors.
  • the immunotherapeutic constructs disclosed herein can be formulated into topical formulations.
  • Several vehicles known in the art can be mixed with the construct, e.g., Aquaphor (ointment-based) and Carbopol (gel-based).
  • Heat or surfactant e.g., Polysorbate 80 (Tween 80) as an emulsifier
  • Tween 80 Polysorbate 80
  • Methods to enhance penetration simultaneously can be used, such as ultrasound and microneedle rollers (e.g., Dermaroller® with the needle height ranging from 0.5 mm to 1.5 mm).
  • Application of microneedles with needle height as short as 0.5 mm can enhance penetration of topical siRNA-nanoparticle formulation when tested in pig skin ( FIG. 30 ) and in mice ( FIG. 31 ).
  • FIG. 30 shows that microneedle roller enhances penetration of siRNA nanoparticle construct when tested in pig skin, which is similar in thickness to human skin.
  • Pig skins were incubated with the formulation (Dy677-siSCR-NP in Aquaphor) for 1.5 h (37° C.; 5% 002). After 1.5 hours, a skin punch was taken from the treated area and processed for fluorescent imaging using a standard approach. Significant enhancement in skin penetration with a microneedle roller was observed. While siRNA signal (arrows) was confined to the outer surface of the pig skin when siRNA-NP in Aquaphor was given without a roller, we observed siRNA signal (arrows) past the epidermis down to the dermis layer with microneedle pre-application ( FIG. 30 ).
  • FIG. 31 shows that microneedle roller enhances topical delivery of siRNA-NP.
  • mice were shaved one day before treatment.
  • Dy677-siSCR-NP (0.72 nmol siRNA) was mixed with 100 ⁇ L of 2.5% Tween-Aquaphor (per one application).
  • a dermal microroller was applied to only one side of the back in four directions consistently, while the other side was not pre-treated.
  • the mixture was applied to the shaved area (approximately 2 cm 2 application area) with and without microneedle pre-treatments for comparison. After 1.5 hr of treatment time, treated skin samples were harvested and processed for imaging.
  • FIG. 32 show the resulting gene knockdown at 3 days after microroller+topical siRNA nanoconstruct application.
  • one intradermal injection of siEGFR-NP resulted in 40% EGFR knock down versus saline treated groups ( FIG. 32B ).
  • Microneedle form of AIRISE-02. The use of dissolvable microneedles based on dextran, amylopectin, PVP, PEG, methylcellulose, chitosan, or other polymers or compounds known in the arts were explored for microneedle fabrications, as shown in FIG. 33 , which allow for painless in-home treatment and are highly effective at delivering AIRISE-02 owing to high needle density (100 needles per 1 cm 2 ).
  • a dextran solution 300 mg/ml in water
  • NP loaded with Dy677-conjugated siRNA was cast onto a microneedle mold. The solution was centrifuged or vacuumed to fill the mold compactly.
  • microneedle was dried by air, desiccator, vacuum oven, fridge, or combination thereof and removed from the mold. Heights of the needles varied from 300 to 800 microns depending on the templates and optimization. siRNA-NP was successfully loaded into these needle arrays (at about 0.5 nmol siRNA per array) and the needles were fully dissolved within 5 min after applying to pig skins. Different dissolving time can be engineered by varying the ingredients of the microneedles. Microneedle patches of different shape and forms can also be manufactured with different templates.
  • Example 6 Different nanoparticle materials can be used to deliver the disclosed cargo combinations and produce similar immunotherapeutic effects.
  • siSTAT3 and CpG were loaded on cationic lipid particles (Dharmafect; commercially available) and administered to mice in the same manner as Example 1 (AIRISE-02 based on mesoporous silica). It was found that CpG and siSTAT3 delivered with the cationic lipid particles also yielded an in situ tumor vaccination/immunostimulatory effect ( FIG. 11 ). A similar outcome was also obtained using jetPEI (commercially available PEI-based transfection agent that reached clinical stages) as the delivery system. This shows the versatility of the disclosed cargo combination and agnostic nature in terms of delivery platform.
  • jetPEI commercially available PEI-based transfection agent that reached clinical stages
  • the layer-by-layer functionalized mesoporous silica nanoparticles show better siRNA knock down activity in immune cells (e.g., primary dendritic cells) than lipid counterpart ( FIG. 12 ).
  • each embodiment disclosed herein can comprise, consist essentially of or consist of its particular stated element, step, ingredient or component.
  • the terms “include” or “including” should be interpreted to recite: “comprise, consist of, or consist essentially of.”
  • the transition term “comprise” or “comprises” means includes and allows for the inclusion of unspecified elements, steps, ingredients, or components, even in major amounts.
  • the transitional phrase “consisting of” excludes any element, step, ingredient or component not specified.
  • the transition phrase “consisting essentially of” limits the scope of the embodiment to the specified elements, steps, ingredients or components and to those that do not materially affect the embodiment.
  • a material effect, in this context, is a measurable reduction in a biological impact (such as an anti-cancer effect) of an immunotherapeutic construct.
  • the term “about” has the meaning reasonably ascribed to it by a person skilled in the art when used in conjunction with a stated numerical value or range, i.e. denoting somewhat more or somewhat less than the stated value or range, to within a range of ⁇ 20% of the stated value; ⁇ 19% of the stated value; ⁇ 18% of the stated value; ⁇ 17% of the stated value; ⁇ 16% of the stated value; ⁇ 15% of the stated value; ⁇ 14% of the stated value; ⁇ 13% of the stated value; ⁇ 12% of the stated value; ⁇ 11% of the stated value; ⁇ 10% of the stated value; ⁇ 9% of the stated value; ⁇ 8% of the stated value; ⁇ 7% of the stated value; ⁇ 6% of the stated value; ⁇ 5% of the stated value; ⁇ 4% of the stated value; ⁇ 3% of the stated value; ⁇ 2% of the stated value; or ⁇ 1% of the stated value.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Organic Chemistry (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Nanotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Inorganic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Optics & Photonics (AREA)
  • Virology (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
US17/625,330 2019-07-12 2020-07-12 Immunotherapeutic constructs and methods of their use Pending US20220249389A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US17/625,330 US20220249389A1 (en) 2019-07-12 2020-07-12 Immunotherapeutic constructs and methods of their use

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962873762P 2019-07-12 2019-07-12
US17/625,330 US20220249389A1 (en) 2019-07-12 2020-07-12 Immunotherapeutic constructs and methods of their use
PCT/US2020/041844 WO2021011496A1 (en) 2019-07-12 2020-07-13 Immunotherapeutic constructs and methods of their use

Publications (1)

Publication Number Publication Date
US20220249389A1 true US20220249389A1 (en) 2022-08-11

Family

ID=74102552

Family Applications (3)

Application Number Title Priority Date Filing Date
US17/625,330 Pending US20220249389A1 (en) 2019-07-12 2020-07-12 Immunotherapeutic constructs and methods of their use
US16/999,948 Active US11235058B2 (en) 2019-07-12 2020-08-21 Immunotherapeutic constructs and methods of their use
US17/549,817 Pending US20220096628A1 (en) 2019-07-12 2021-12-13 Immunotherapeutic constructs and methods of their use

Family Applications After (2)

Application Number Title Priority Date Filing Date
US16/999,948 Active US11235058B2 (en) 2019-07-12 2020-08-21 Immunotherapeutic constructs and methods of their use
US17/549,817 Pending US20220096628A1 (en) 2019-07-12 2021-12-13 Immunotherapeutic constructs and methods of their use

Country Status (8)

Country Link
US (3) US20220249389A1 (ko)
EP (1) EP3999111A4 (ko)
JP (1) JP2022540867A (ko)
KR (1) KR20220035160A (ko)
CN (1) CN114096274A (ko)
AU (1) AU2020315590A1 (ko)
CA (1) CA3139722A1 (ko)
MX (1) MX2022000544A (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024049734A1 (en) * 2022-08-29 2024-03-07 Celanese Eva Performance Polymers Llc Implantable device for intratumorally administering a therapeutic agent

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3996750A4 (en) 2019-07-12 2024-01-03 Oregon Health & Science University THERAPEUTIC CONSTRUCTS FOR SIMULTANEOUS ADMINISTRATION OF MITOTIC HIBITOR AND IMMUNE CHECKPOINT INHIBITOR
US20240066119A1 (en) * 2021-01-13 2024-02-29 University Of Maryland, College Park Methods and platforms for eliciting an immune response in the treatment of cancer and compositions and vaccines relating thereto
CN115060777B (zh) * 2022-07-08 2023-06-23 江苏理工学院 同时检测马拉硫磷和氧化乐果的比率电化学适配体传感器及制备方法和应用
CN117085001B (zh) * 2023-10-18 2024-02-13 中国医学科学院药用植物研究所 一种双重靶向核壳结构纳米载药颗粒的制备方法及应用
CN117731616A (zh) * 2023-12-18 2024-03-22 中山大学孙逸仙纪念医院 一种负载肿瘤抑制基因siRNA的谷胱甘肽响应纳米材料

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406705B1 (en) 1997-03-10 2002-06-18 University Of Iowa Research Foundation Use of nucleic acids containing unmethylated CpG dinucleotide as an adjuvant
US7375096B1 (en) 1998-12-04 2008-05-20 California Institute Of Technology Method of preparing a supramolecular complex containing a therapeutic agent and a multi-dimensional polymer network
US7098192B2 (en) * 1999-04-08 2006-08-29 Isis Pharmaceuticals, Inc. Antisense oligonucleotide modulation of STAT3 expression
US6586524B2 (en) 2001-07-19 2003-07-01 Expression Genetics, Inc. Cellular targeting poly(ethylene glycol)-grafted polymeric gene carrier
US6740336B2 (en) 2002-10-04 2004-05-25 Mirus Corporation Process for generating multilayered particles
US8399025B2 (en) 2004-06-04 2013-03-19 Board Of Regents, The University Of Texas System Polyamine modified particles
US20060293396A1 (en) 2005-01-14 2006-12-28 Eastman Kodak Company Amine polymer-modified nanoparticulate carriers
EP1973608A1 (en) 2005-12-14 2008-10-01 Cytos Biotechnology AG Immunostimulatory nucleic acid packaged particles for the treatment of hypersensitivity
AU2007345648A1 (en) * 2007-01-26 2008-08-07 City Of Hope Methods and compositions for the treatment of cancer or other diseases
CN107582526A (zh) * 2009-08-12 2018-01-16 希格默伊德药业有限公司 包含聚合物基质和油相的免疫调节组合物
US20120207795A1 (en) * 2010-07-13 2012-08-16 The Regents Of The University Of California Cationic polymer coated mesoporous silica nanoparticles and uses thereof
US9511151B2 (en) 2010-11-12 2016-12-06 Uti Limited Partnership Compositions and methods for the prevention and treatment of cancer
RU2014106024A (ru) 2011-07-19 2015-08-27 Юниверсити Оф Айдахо Варианты осуществления зонда и способы направленного действия на нуклеиновые кислоты
CA2820236C (en) 2012-06-15 2022-07-19 The Royal Institution For The Advancement Of Learning/Mcgill University Non-viral nanoparticle-based delivery system
PL2948179T3 (pl) * 2013-01-25 2019-10-31 Nanobiotix Kompozycje zawierające nanocząstki tlenku hafnu(iv) lub tlenku renu(iv) w połączeniu z promieniowaniem jonizującym do leczenia nowotworu
EP3046547A4 (en) 2013-09-18 2017-05-24 Stc.Unm Core and surface modification of mesoporous silica nanoparticles to achieve cell specific targeting in vivo
CA2987519A1 (en) * 2013-11-01 2015-05-07 Yale University Delivery vehicles
WO2015077657A2 (en) 2013-11-22 2015-05-28 City Of Hope Stat3 inhibitors and uses thereof
JP6701088B2 (ja) * 2014-03-19 2020-05-27 インフィニティー ファーマシューティカルズ， インコーポレイテッド Ｐｉ３ｋ−ガンマ媒介障害の治療で使用するための複素環式化合物
AU2015243380B2 (en) 2014-04-11 2020-05-14 Medimmune, Llc Conjugated compounds comprising cysteine-engineered antibodies
KR101799557B1 (ko) 2014-07-22 2017-11-20 주식회사 레모넥스 생리활성 물질 또는 단백질 전달용 조성물 및 이의 용도
US20160090603A1 (en) 2014-09-30 2016-03-31 Sandia Corporation Delivery platforms for the domestication of algae and plants
WO2016149378A1 (en) 2015-03-16 2016-09-22 Pdx Pharmaceuticals, Llc Cross-linked polymer modified nanoparticles
CA2979712C (en) 2015-03-25 2024-01-23 The Regents Of The University Of Michigan Nanoparticle compositions for delivery of biomacromolecules
EP3603661A3 (en) * 2015-04-22 2020-04-01 CureVac AG Rna containing composition for treatment of tumor diseases
EP3303598A4 (en) 2015-05-26 2019-01-23 Ramot at Tel-Aviv University Ltd. TARGETED LIPID PARTICLES FOR SYSTEMIC DELIVERY OF NUCLEIC ACID MOLECULES TO LEUKOCYTES
EP3878465A1 (en) 2015-07-29 2021-09-15 Novartis AG Combination therapies comprising antibody molecules to tim-3
WO2017040660A1 (en) 2015-08-31 2017-03-09 Oncomed Pharmaceuticals, Inc. Combination therapy for treatment of disease
WO2017106129A1 (en) 2015-12-16 2017-06-22 Merck Sharp & Dohme Corp. Anti-lag3 antibodies and antigen-binding fragments
EP4218739A3 (en) 2016-01-08 2023-08-09 The Regents of The University of California Mesoporous silica nanoparticles with lipid bilayer coating for cargo delivery
EP3442542A4 (en) * 2016-03-15 2020-01-15 North Carolina State University NANOPARTICLE, DOSAGE FORM WITH CONTROLLED RELEASE, AND METHOD FOR RELEASING AN IMMUNOTHERAPEUTIC
EP3458474B1 (en) 2016-05-18 2022-07-06 ModernaTX, Inc. Combinations of mrnas encoding immune modulating polypeptides and uses thereof
SG11201909516VA (en) * 2017-04-14 2019-11-28 Tollnine Inc Immunomodulating polynucleotides, antibody conjugates thereof, and methods of their use
WO2019006371A1 (en) * 2017-06-30 2019-01-03 City Of Hope COMPOSITIONS AND METHODS FOR MODULATING MACROPHAGE ACTIVITY
US20190048049A1 (en) 2017-08-10 2019-02-14 Cerenis Therapeutics Holding Sa Cargomers
US20190374479A1 (en) 2018-06-12 2019-12-12 The Brigham And Women's Hospital, Inc. Particles for delivery of biomolecules
EP3996750A4 (en) * 2019-07-12 2024-01-03 Oregon Health & Science University THERAPEUTIC CONSTRUCTS FOR SIMULTANEOUS ADMINISTRATION OF MITOTIC HIBITOR AND IMMUNE CHECKPOINT INHIBITOR

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024049734A1 (en) * 2022-08-29 2024-03-07 Celanese Eva Performance Polymers Llc Implantable device for intratumorally administering a therapeutic agent

Also Published As

Publication number Publication date
CN114096274A (zh) 2022-02-25
US11235058B2 (en) 2022-02-01
AU2020315590A1 (en) 2022-01-20
EP3999111A1 (en) 2022-05-25
US20220096628A1 (en) 2022-03-31
JP2022540867A (ja) 2022-09-20
EP3999111A4 (en) 2024-02-28
MX2022000544A (es) 2022-04-18
CA3139722A1 (en) 2021-01-21
KR20220035160A (ko) 2022-03-21
US20210008198A1 (en) 2021-01-14

Similar Documents

Publication Publication Date Title
US11235058B2 (en) Immunotherapeutic constructs and methods of their use
WO2021011496A1 (en) Immunotherapeutic constructs and methods of their use
Hou et al. Manganese-based nanoactivator optimizes cancer immunotherapy via enhancing innate immunity
US11679082B2 (en) Therapeutic constructs for co-delivery of mitotic kinase inhibitor and immune checkpoint inhibitor
JP6730285B2 (ja) Ｒｎａ干渉組成物及び悪性腫瘍のための方法
US20220054633A1 (en) Nanoparticles, controlled-release dosage forms, and methods for delivering an immunotherapeutic agent
US11090367B2 (en) Restoration of tumor suppression using mRNA-based delivery system
US20220111029A1 (en) Polymeric nanovaccines and uses thereof
WO2017095751A1 (en) Compositions and methods for modulating cancer cell metabolism
Dai et al. Nanomedicines modulating myeloid-derived suppressor cells for improving cancer immunotherapy
WO2014165296A1 (en) Methods and formulations to achieve tumor targeted double stranded rna mediated cell death
KR101949507B1 (ko) Kras를 표적으로 하는 핵산 함유 약제학적 조성물 및 그 제조방법
Peres Intracellularly-Targeted Nanoplatform to Target Dendritic Cells and for Immunomodulation
AU2022395048A1 (en) Spherical nucleic acids for cgas-sting and stat3 pathway modulation for the immunotherapeutic treatment of cancer

Legal Events

Date Code Title Description
AS Assignment

Owner name: PDX PHARMACEUTICALS, INC., OREGON

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:NGAMCHERDTRAKUL, WORAPOL;REDA, MOATAZ;REEL/FRAME:058805/0422

Effective date: 20201110

Owner name: OREGON HEALTH & SCIENCE UNIVERSITY, OREGON

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:YANTASEE, WASSANA;LUND, AMANDA;SIGNING DATES FROM 20201110 TO 20201111;REEL/FRAME:058805/0416

STPP Information on status: patent application and granting procedure in general

Free format text: DOCKETED NEW CASE - READY FOR EXAMINATION