US20100265575A1 - Microscope - Google Patents

Microscope Download PDF

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Publication number
US20100265575A1
US20100265575A1 US12/809,136 US80913608A US2010265575A1 US 20100265575 A1 US20100265575 A1 US 20100265575A1 US 80913608 A US80913608 A US 80913608A US 2010265575 A1 US2010265575 A1 US 2010265575A1
Authority
US
United States
Prior art keywords
plane
bessel
light
cylindrical lens
focus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US12/809,136
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English (en)
Inventor
Helmut Lippert
Ralf Wolleschensky
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Carl Zeiss Microscopy GmbH
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of US20100265575A1 publication Critical patent/US20100265575A1/en
Assigned to CARL ZEISS MICROSCOPY GMBH reassignment CARL ZEISS MICROSCOPY GMBH CHANGE OF NAME (SEE DOCUMENT FOR DETAILS). Assignors: CARL ZEISS MICROIMAGING GMBH
Assigned to ALBERT-LUDWIGS-UNIVERSITY OF FREIBURG reassignment ALBERT-LUDWIGS-UNIVERSITY OF FREIBURG ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: FAHRBACH, FLORIAN, ROHRBACH, ALEXANDER
Assigned to CARL ZEISS MICROSCOPY GMBH reassignment CARL ZEISS MICROSCOPY GMBH ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: ALBERT-LUDWIGS-UNIVERSITY OF FREIBURG
Assigned to CARL ZEISS MICROSCOPY GMBH reassignment CARL ZEISS MICROSCOPY GMBH ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: WOLLESCHENSKY, RALF, LIPPERT, HELMUT, DR.
Abandoned legal-status Critical Current

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    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
    • G02B27/0911—Anamorphotic systems
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/10—Condensers affording dark-field illumination
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/0816—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light by means of one or more reflecting elements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
US12/809,136 2007-12-20 2008-12-10 Microscope Abandoned US20100265575A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102007063274.8 2007-12-20
DE102007063274.8A DE102007063274B8 (de) 2007-12-20 2007-12-20 Mikroskop
PCT/EP2008/010455 WO2009080210A2 (fr) 2007-12-20 2008-12-10 Microscope

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/010455 A-371-Of-International WO2009080210A2 (fr) 2007-12-20 2008-12-10 Microscope

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US14/249,921 Continuation US10054780B2 (en) 2007-12-20 2014-04-10 Microscope

Publications (1)

Publication Number Publication Date
US20100265575A1 true US20100265575A1 (en) 2010-10-21

Family

ID=40467201

Family Applications (2)

Application Number Title Priority Date Filing Date
US12/809,136 Abandoned US20100265575A1 (en) 2007-12-20 2008-12-10 Microscope
US14/249,921 Active US10054780B2 (en) 2007-12-20 2014-04-10 Microscope

Family Applications After (1)

Application Number Title Priority Date Filing Date
US14/249,921 Active US10054780B2 (en) 2007-12-20 2014-04-10 Microscope

Country Status (5)

Country Link
US (2) US20100265575A1 (fr)
EP (3) EP2235577B1 (fr)
JP (1) JP5658038B2 (fr)
DE (1) DE102007063274B8 (fr)
WO (1) WO2009080210A2 (fr)

Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110116163A1 (en) * 2007-10-18 2011-05-19 Vincent Loriette System for the structured illumination of a sample
US20110304723A1 (en) * 2010-06-14 2011-12-15 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US20120099190A1 (en) * 2010-10-22 2012-04-26 Leica Microsystems Cms Gmbh SPIM Microscope with a sequential light sheet
US20120176674A1 (en) * 2009-09-24 2012-07-12 Carl Zeiss Microimaging Gmbh Microscope
US20120206798A1 (en) * 2011-02-14 2012-08-16 European Molecular Biology Laboratory (Embl) Light-pad microscope for high-resolution 3d fluorescence imaging and 2d fluctuation spectroscopy
US20130107358A1 (en) * 2011-10-28 2013-05-02 Leica Mircosystems Cms Gmbh Method and system for illuminating a sample
US20130286181A1 (en) * 2010-06-14 2013-10-31 Howard Hughes Medical Institute Structured plane illumination microscopy
US20150022881A1 (en) * 2013-07-22 2015-01-22 Universitat Politecnca De Catalunya Light sheet-based imaging device with extended depth of field
US20160048014A1 (en) * 2013-03-22 2016-02-18 Leica Microsystems Cms Gmbh SPIM Arrangement
WO2016054118A1 (fr) * 2014-09-29 2016-04-07 Howard Hughes Medical Institute Microscopie à éclairage structuré non linéaire
US20160161410A1 (en) * 2012-10-09 2016-06-09 Howard Hughes Medical Institute Multiview Light-Sheet Microscopy
CN105676436A (zh) * 2016-04-01 2016-06-15 哈尔滨理工大学 一种小物距显微系统垂直照明装置
US20160320301A1 (en) * 2013-12-17 2016-11-03 Leica Microsystems Cms Gmbh Method and apparatus for investigating a sample by means of optical projection tomography
US20180011303A1 (en) * 2015-05-22 2018-01-11 The Hong Kong University Of Science And Technology Methods and systems for generating non-diffracting light sheets for multicolor fluorescence microscopy
CN107884379A (zh) * 2017-12-18 2018-04-06 北京赛尔怡美科技有限公司 光片照明成像装置
WO2018089839A1 (fr) * 2016-11-10 2018-05-17 The Trustees Of Columbia University In The City Of New York Procédés d'imagerie rapide de grands échantillons à haute résolution
US10088657B2 (en) 2014-06-11 2018-10-02 Hans-Ulrich Dodt Light sheet microscopy using meso-optical elements
US20180284411A1 (en) * 2017-03-30 2018-10-04 Olympus Corporation Microscope apparatus
US10247934B2 (en) * 2014-12-19 2019-04-02 Carl Zeiss Microscopy Gmbh Method for examining a specimen by means of light sheet microscopy
US10379335B2 (en) * 2016-05-09 2019-08-13 Olympus Corporation Illumination setting method, light sheet microscope apparatus, and recording medium
US10539772B2 (en) 2013-10-09 2020-01-21 Howard Hughes Medical Institute Multiview light-sheet microscopy
CN111103678A (zh) * 2018-10-28 2020-05-05 西湖大学 晶格光片显微镜和在晶格光片显微镜中平铺晶格光片的方法
US10795144B2 (en) 2014-12-06 2020-10-06 Howard Hughes Medical Institute Microscopy with structured plane illumination and point accumulation for imaging and nanoscale topography
US11320640B2 (en) 2016-06-24 2022-05-03 Howard Hughes Medical Institute Automated adjustment of light sheet geometry in a microscope
CN114815561A (zh) * 2021-01-19 2022-07-29 统雷有限公司 光学图像生成系统和生成光学图像的方法

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10257423A1 (de) 2002-12-09 2004-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL) Mikroskop
PT2542145T (pt) * 2010-03-05 2020-11-04 Massachusetts Gen Hospital Sistemas, métodos e meios acessíveis por computador que proporcionam imagens microscópicas de pelo menos uma estrutura anatómica numa resolução particular
WO2012003259A1 (fr) * 2010-06-30 2012-01-05 The Government Of The U.S.A., Represented By The Secretary, Department Of Health And Human Services Système et procédé de production de rideaux de lumière non diffractants au moyen d'une pluralité de faisceaux optiques non diffractants minimalement interférants, se chevauchant spatialement
CN101893755B (zh) * 2010-07-06 2012-07-25 中国科学院西安光学精密机械研究所 使用四棱锥镜产生结构照明的荧光显微方法和装置
JP6166776B2 (ja) * 2012-04-03 2017-07-19 ユニバーシティー コート オブ ザ ユニバーシティー オブ セイント アンドリューズＵｎｉｖｅｒｓｉｔｙ Ｃｏｕｒｔ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｓｔ Ａｎｄｒｅｗｓ 拡張ボリュームの高分解能イメージング
DE102012013163B4 (de) 2012-07-02 2022-08-25 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren zur Lichtscheibenmikroskopie
CN104677871A (zh) * 2015-02-27 2015-06-03 中国科学院自动化研究所 多光子激发光片照明显微成像系统
GB201508376D0 (en) * 2015-05-15 2015-07-01 Univ St Andrews Light sheet imaging
DE102016108384B3 (de) * 2016-05-04 2017-11-09 Leica Microsystems Cms Gmbh Vorrichtung und Verfahren zur lichtblattartigen Beleuchtung einer Probe
DE102016115844A1 (de) * 2016-07-01 2018-01-04 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Anordnung zur Erzeugung eines Bessel-Strahls
LU93143B1 (de) 2016-07-06 2018-03-05 Leica Microsystems Verfahren zum Untersuchen einer Probe sowie Vorrichtung zum Ausführen eines solchen Verfahrens
JP7021190B2 (ja) 2016-08-15 2022-02-16 ライカ マイクロシステムズ シーエムエス ゲゼルシャフト ミット ベシュレンクテル ハフツング ライトシート顕微鏡
CN110168423B (zh) 2016-11-11 2022-08-05 徕卡显微系统复合显微镜有限公司 照明装置和用于在显微镜中照明的方法和显微镜
LU93332B1 (de) * 2016-12-02 2018-06-21 Leica Microsystems Beleuchtungsanordnung und Verfahren für die Beleuchtung in einem Mikroskop und Mikroskop
DE102016124612A1 (de) * 2016-12-16 2018-06-21 Carl Zeiss Microscopy Gmbh Segmentierte Optik für ein Beleuchtungsmodul zur winkelselektiven Beleuchtung
DE102019109607A1 (de) 2018-08-10 2020-02-13 Leica Microsystems Cms Gmbh Beleuchtungsanordnung für ein Mikroskop
DE102018222839A1 (de) * 2018-08-10 2020-02-13 Leica Microsystems Cms Gmbh Beleuchtungsanordnung für ein Mikroskop, Mikroskop und Verfahren zur Beleuchtung eines Probenvolumens in einem Mikroskop
DE102020128524A1 (de) 2020-10-29 2022-05-05 Carl Zeiss Microscopy Gmbh Lichtblattmikroskop und Verfahren der Lichtblattmikroskopie
DE102022116517A1 (de) 2022-07-01 2024-01-04 Esg Elektroniksystem- Und Logistik-Gmbh Vorrichtung zur detektion einer einfallsrichtung und einer wellenlänge einfallender laserstrahlung

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4826299A (en) * 1987-01-30 1989-05-02 Canadian Patents And Development Limited Linear deiverging lens
US5602643A (en) * 1996-02-07 1997-02-11 Wyko Corporation Method and apparatus for correcting surface profiles determined by phase-shifting interferometry according to optical parameters of test surface
US5978109A (en) * 1992-03-19 1999-11-02 Matsushita Electric Ind Co Ltd Superresolution scanning optical device
US6281993B1 (en) * 1998-03-30 2001-08-28 International Business Machines Corporation Phase shifting element for optical information processing storing systems

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3614888A1 (de) 1986-05-02 1987-11-05 Hell Rudolf Dr Ing Gmbh Optische anordnung zur zeilenweisen beleuchtung von abtastvorlagen
JPH04171415A (ja) * 1990-11-02 1992-06-18 Nikon Corp 長焦点深度高分解能照射光学系
EP0627643B1 (fr) 1993-06-03 1999-05-06 Hamamatsu Photonics K.K. Système optique de balayage à laser utilisant un axicon
JPH0815156A (ja) * 1993-06-03 1996-01-19 Hamamatsu Photonics Kk レーザスキャン光学系及びレーザスキャン光学装置
GB0118306D0 (en) * 2001-07-27 2001-09-19 Isis Innovation Method of,and apparatus for,generating a focussed light beam
US6888148B2 (en) * 2001-12-10 2005-05-03 Carl Zeiss Jena Gmbh Arrangement for the optical capture of excited and /or back scattered light beam in a sample
JP3828799B2 (ja) * 2001-12-14 2006-10-04 独立行政法人科学技術振興機構 光学系の薄層斜光照明法
DE10257423A1 (de) 2002-12-09 2004-06-24 Europäisches Laboratorium für Molekularbiologie (EMBL) Mikroskop
AU2003295694A1 (en) 2002-12-11 2004-06-30 Eli Lilly And Company Junctional adhesion molecule splice variants
DE10338472B4 (de) 2003-08-21 2020-08-06 Carl Zeiss Meditec Ag Optisches Abbildungssystem mit erweiterter Schärfentiefe
DE102004034957A1 (de) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh Anordnung zur mikroskopischen Beobachtung und/oder Detektion und Verwendung
DE102005027077C5 (de) 2004-11-04 2021-01-28 Leica Microsystems Cms Gmbh Lichtscheibenmikroskop
EP1907826B2 (fr) * 2005-07-22 2020-11-25 Carl Zeiss MicroImaging GmbH Microscopie a luminescence a resolution amelioree
JP2007279287A (ja) 2006-04-05 2007-10-25 Nikon Corp 構造化照明光学系、及びそれを備えた構造化照明顕微鏡
DE102007015063B4 (de) 2007-03-29 2019-10-17 Carl Zeiss Microscopy Gmbh Optische Anordnung zum Erzeugen eines Lichtblattes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4826299A (en) * 1987-01-30 1989-05-02 Canadian Patents And Development Limited Linear deiverging lens
US5978109A (en) * 1992-03-19 1999-11-02 Matsushita Electric Ind Co Ltd Superresolution scanning optical device
US5602643A (en) * 1996-02-07 1997-02-11 Wyko Corporation Method and apparatus for correcting surface profiles determined by phase-shifting interferometry according to optical parameters of test surface
US6281993B1 (en) * 1998-03-30 2001-08-28 International Business Machines Corporation Phase shifting element for optical information processing storing systems

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Botcherby et al. "Scanning two photon fluorescence microscopy with extended depth of field" 07/26/2006, Optics Communications Vol. 268, pages 253-260 *
Breuninger et al. "Lateral Modulation boosts image quality in a single plane illumination fluorescence microscopy," 7/1/2007, Optics Letters Vol. 32 Pages 1938-1940 *
Lei et al. "Structuring by Multi-Beam interference using symmetric pyramids," 05/13/2006, Optics Express Vol. 14 pages 5803- 5811 *
Lei et al. "Structuring by Multi-Beam interference using symmetric pyramids," 05/13/2006, Optics Express Vol. 14 pages 5803-5811 *

Cited By (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110116163A1 (en) * 2007-10-18 2011-05-19 Vincent Loriette System for the structured illumination of a sample
US8289622B2 (en) * 2007-10-18 2012-10-16 Centre National de la Recherche Scientifique—CNRS System for the structured illumination of a sample
US20120176674A1 (en) * 2009-09-24 2012-07-12 Carl Zeiss Microimaging Gmbh Microscope
US8792162B2 (en) * 2009-09-24 2014-07-29 Carl Zeiss Microscopy Gmbh Microscope with illumination switching for capturing sample images during detector integration time
US20140284461A1 (en) * 2010-06-14 2014-09-25 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US9223125B2 (en) * 2010-06-14 2015-12-29 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US9448395B2 (en) 2010-06-14 2016-09-20 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US10051240B2 (en) * 2010-06-14 2018-08-14 Howard Hughes Medical Institute Structured plane illumination microscopy
US20130286181A1 (en) * 2010-06-14 2013-10-31 Howard Hughes Medical Institute Structured plane illumination microscopy
US10721441B2 (en) 2010-06-14 2020-07-21 Howard Hughes Medical Institute Structured plane illumination microscopy
US8711211B2 (en) * 2010-06-14 2014-04-29 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US10509217B2 (en) 2010-06-14 2019-12-17 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US9477074B2 (en) * 2010-06-14 2016-10-25 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US20140285653A1 (en) * 2010-06-14 2014-09-25 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US20110304723A1 (en) * 2010-06-14 2011-12-15 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US9791685B2 (en) 2010-06-14 2017-10-17 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US9891421B2 (en) 2010-06-14 2018-02-13 Howard Hughes Medical Institute Bessel beam plane illumination microscope
US9116354B2 (en) * 2010-10-22 2015-08-25 Leica Microsystems Cms Gmbh SPIM microscope with a STED light sheet
US9057879B2 (en) * 2010-10-22 2015-06-16 Leica Microsystems Cms Gmbh SPIM microscope with a sequential light sheet
US20120099190A1 (en) * 2010-10-22 2012-04-26 Leica Microsystems Cms Gmbh SPIM Microscope with a sequential light sheet
US20120098949A1 (en) * 2010-10-22 2012-04-26 Leica Microsystems Cms Gmbh Spim microscope with a sted light sheet
US20120206798A1 (en) * 2011-02-14 2012-08-16 European Molecular Biology Laboratory (Embl) Light-pad microscope for high-resolution 3d fluorescence imaging and 2d fluctuation spectroscopy
US10908403B2 (en) * 2011-02-14 2021-02-02 European Molecular Biology Laboratory (Embl) Light-pad microscope for high-resolution 3D fluorescence imaging and 2D fluctuation spectroscopy
CN103649813A (zh) * 2011-02-14 2014-03-19 欧洲分子生物学实验室(Embl) 用于空间解析荧光相关光谱学的光垫显微镜
US20130107358A1 (en) * 2011-10-28 2013-05-02 Leica Mircosystems Cms Gmbh Method and system for illuminating a sample
US9104020B2 (en) * 2011-10-28 2015-08-11 Leica Microsystems Cms Gmbh Method and system for illuminating a sample
US20160161410A1 (en) * 2012-10-09 2016-06-09 Howard Hughes Medical Institute Multiview Light-Sheet Microscopy
US10739266B2 (en) 2012-10-09 2020-08-11 Howard Hughes Medical Institute Multiview light-sheet microscopy
US10386302B2 (en) * 2012-10-09 2019-08-20 Howard Hughes Medical Institute Multiview light-sheet microscopy
US20160048014A1 (en) * 2013-03-22 2016-02-18 Leica Microsystems Cms Gmbh SPIM Arrangement
US10215974B2 (en) * 2013-03-22 2019-02-26 Leica Microsystems Cms Gmbh Selective/single plane illumination microscopy (SPIM) arrangement
US9581798B2 (en) * 2013-07-22 2017-02-28 Fundacio Institut De Ciencies Fotoniques Light sheet-based imaging device with extended depth of field
US20150022881A1 (en) * 2013-07-22 2015-01-22 Universitat Politecnca De Catalunya Light sheet-based imaging device with extended depth of field
US10539772B2 (en) 2013-10-09 2020-01-21 Howard Hughes Medical Institute Multiview light-sheet microscopy
US10429301B2 (en) * 2013-12-17 2019-10-01 Leica Microsystems Cms Gmbh Method and apparatus for investigating a sample by means of optical projection tomography
US20160320301A1 (en) * 2013-12-17 2016-11-03 Leica Microsystems Cms Gmbh Method and apparatus for investigating a sample by means of optical projection tomography
US10088657B2 (en) 2014-06-11 2018-10-02 Hans-Ulrich Dodt Light sheet microscopy using meso-optical elements
US10247672B2 (en) 2014-09-29 2019-04-02 Howard Hughes Medical Institute Non-linear structured illumination microscopy
WO2016054118A1 (fr) * 2014-09-29 2016-04-07 Howard Hughes Medical Institute Microscopie à éclairage structuré non linéaire
US10795144B2 (en) 2014-12-06 2020-10-06 Howard Hughes Medical Institute Microscopy with structured plane illumination and point accumulation for imaging and nanoscale topography
US10247934B2 (en) * 2014-12-19 2019-04-02 Carl Zeiss Microscopy Gmbh Method for examining a specimen by means of light sheet microscopy
US10942346B2 (en) * 2015-05-22 2021-03-09 The Hong Kong University Of Science And Technology Methods and systems for generating non-diffracting light sheets for multicolor fluorescence microscopy
US20180011303A1 (en) * 2015-05-22 2018-01-11 The Hong Kong University Of Science And Technology Methods and systems for generating non-diffracting light sheets for multicolor fluorescence microscopy
CN105676436A (zh) * 2016-04-01 2016-06-15 哈尔滨理工大学 一种小物距显微系统垂直照明装置
US10379335B2 (en) * 2016-05-09 2019-08-13 Olympus Corporation Illumination setting method, light sheet microscope apparatus, and recording medium
US11320640B2 (en) 2016-06-24 2022-05-03 Howard Hughes Medical Institute Automated adjustment of light sheet geometry in a microscope
WO2018089839A1 (fr) * 2016-11-10 2018-05-17 The Trustees Of Columbia University In The City Of New York Procédés d'imagerie rapide de grands échantillons à haute résolution
US10884227B2 (en) 2016-11-10 2021-01-05 The Trustees Of Columbia University In The City Of New York Rapid high-resolution imaging methods for large samples
US11506877B2 (en) 2016-11-10 2022-11-22 The Trustees Of Columbia University In The City Of New York Imaging instrument having objective axis and light sheet or light beam projector axis intersecting at less than 90 degrees
US10684457B2 (en) * 2017-03-30 2020-06-16 Olympus Corporation Microscope apparatus
US20180284411A1 (en) * 2017-03-30 2018-10-04 Olympus Corporation Microscope apparatus
CN107884379A (zh) * 2017-12-18 2018-04-06 北京赛尔怡美科技有限公司 光片照明成像装置
CN111103678A (zh) * 2018-10-28 2020-05-05 西湖大学 晶格光片显微镜和在晶格光片显微镜中平铺晶格光片的方法
CN114815561A (zh) * 2021-01-19 2022-07-29 统雷有限公司 光学图像生成系统和生成光学图像的方法

Also Published As

Publication number Publication date
WO2009080210A3 (fr) 2009-08-20
DE102007063274B4 (de) 2022-03-10
EP2235577B1 (fr) 2019-06-05
US10054780B2 (en) 2018-08-21
DE102007063274A1 (de) 2009-06-25
JP5658038B2 (ja) 2015-01-21
EP3528029A1 (fr) 2019-08-21
EP3528029B1 (fr) 2020-07-01
DE102007063274B8 (de) 2022-12-15
WO2009080210A2 (fr) 2009-07-02
EP2235577A2 (fr) 2010-10-06
US20150070757A1 (en) 2015-03-12
JP2011507040A (ja) 2011-03-03
EP3754402A1 (fr) 2020-12-23

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