US20070292950A1 - Support for Cultivating Biological Material - Google Patents

Support for Cultivating Biological Material Download PDF

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Publication number
US20070292950A1
US20070292950A1 US11/570,177 US57017705A US2007292950A1 US 20070292950 A1 US20070292950 A1 US 20070292950A1 US 57017705 A US57017705 A US 57017705A US 2007292950 A1 US2007292950 A1 US 2007292950A1
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US
United States
Prior art keywords
container
substrate
sowing
growth
sterile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US11/570,177
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English (en)
Inventor
Jacob Bijl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vitro Plus CV
Vissers Gravendeel Holding BV
Original Assignee
Vitro Plus CV
Vissers Gravendeel Holding BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vitro Plus CV, Vissers Gravendeel Holding BV filed Critical Vitro Plus CV
Assigned to VITRO PLUS, C.V., VISSER 'S-GRAVENDEEL HOLDING B.V. reassignment VITRO PLUS, C.V. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BIJL, JACOB JOHANNES
Publication of US20070292950A1 publication Critical patent/US20070292950A1/en
Abandoned legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers

Definitions

  • the present invention relates to a container and method for cultivating biological material as according to the preamble of claims 1 and 10 respectively, and to the use of the container as according to the preamble of claim 12 .
  • a container for cultivating biological material according to the preamble of claim 1 is known from U.S. Pat. No. 6,032,409.
  • the sowing material is preferably chosen from the group comprising seeds such as vegetable seeds of for instance tomato, cucumber and paprika, seeds of ornamental plants such as Gerbera, Anthurium, Bromelia, Orchid and Spathiphyllum, and seeds of arable crops, shredded and singulated vegetable material, vegetable somatic embryos, fern spores, adventive or axillary shoots or parts thereof.
  • the shredded vegetable material preferably comprises shredded fern parts, ferns, saintpaulia, primula and lily.
  • the adventive or axillary shoots or parts thereof preferably originate from vegetable crops such as tomato, cucumber and paprika.
  • the growth medium preferably comprises water, salts (minerals) and/or sugars, such as an agar medium and preferably a liquid or (highly) diluted agar medium.
  • agar medium preferably a liquid or (highly) diluted agar medium.
  • sugars such as an agar medium and preferably a liquid or (highly) diluted agar medium.
  • At least one side of the container is preferably closed by means of a semi-permeable foil.
  • the advantage hereof is that the container is closed off from the environment, whereby a so-called micro-environment is created.
  • the advantage of such an environment is that the conditions inside the container can be controlled very precisely.
  • the semi-permeable foil plays an important part here, since it can be chosen such that it is for instance permeable to determined gases but not to others (for instance permeable to O 2 to the outside but not to CO 2 ). In this manner the conditions inside the space of the container can thus be set such that they are ideal for the biological material for cultivating, such as sowing material.
  • the carrier preparation comprises agar.
  • the advantage hereof is that it is a very stable medium and that different ingredients such as water, sugars and salts (minerals) can readily be added thereto.
  • a final aspect of the present invention relates to the use of the above described container in the cultivation of biological material.
  • FIG. 2 shows a schematic side view of a container according to the invention provided with a plurality of sub-containers.
  • FIG. 1 shows a side view of a container 1 according to the invention.
  • an inert solid substrate 3 which is provided with growth medium.
  • a quantity of sowing material in carrier material 4 is arranged on the top side of substrate 3 .
  • carrier material 4 is used up and the material continues to grow in substrate 3 .
  • Substrate 3 can then be separated from the container and be transferred into a pot with potting soil or similar substrate.
  • FIG. 2 shows a side view of a container 1 according to the invention.
  • Container 1 is provided with a plurality of sub-containers 6 placed in container 1 .
  • An inert substrate 3 is arranged in each sub-container 6 .
  • a carrier preparation 4 with sowing material therein is arranged on substrate 3 .
  • Container 1 is closed along side walls 2 by means of transparent plastic material.
  • the top side of container 1 is closed using a semi-permeable foil 5 .
  • This foil 5 ensures that the gas composition of inner space 7 of container 1 has the desired composition and humidity. Foil 5 further ensures that the sowing material does not become contaminated with undesired micro-organisms.
  • a valve 8 is placed against one of the walls 2 of container 1 .
  • additional growth medium can however also be added to substrate 3 of sub-containers 6 through this valve 8 .
  • a first group 104 sub-containers were placed under sterile conditions in a container according to the invention.
  • the sub-containers were mutually connected on the top side such that they formed a tray.
  • rockwool Arranged in the sub-containers was rockwool which was provided with a growth medium.
  • Sowing material in a carrier preparation was arranged on the rockwool.
  • the carrier preparation here comprised agar-agar, water, salts and sugar.
  • the sowing material comprised shredded fern parts of the type Nephrolepis exaltata ‘Corditas’.
  • the container, the sub-containers, the rockwool and the carrier preparation with sowing material were all sterile.
  • the container was then closed under sterile conditions using a semi-permeable foil (PET 12/PE30).
  • the container with the sowing material therein was incubated for 40 days at 25° C. and at an interval of 16 hours daylight and 8 hours night.
  • the plants were incubated until they were large enough to be transferred into a so-called transplant tray with potting soil. In this transplant tray they were placed in a greenhouse and kept there until they were large enough to be placed in pots.
  • Table 2 shows the growth of the plants in a non-sterile transplant tray up to the moment the plants can be placed in pots.
  • Table 1 shows clearly that the plants from group 2 need more time to grow before they can be placed in pots. It is further shown that the roots of the plants of group 1 are better developed.
  • Table 2 clearly shows that the rooting time in the transplant tray with potting soil is markedly shorter in the plants of group 1. This table further also shows that the failure percentage of the second group is much higher than that of the first group.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Soil Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
US11/570,177 2004-06-10 2005-06-10 Support for Cultivating Biological Material Abandoned US20070292950A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NL1026379A NL1026379C2 (nl) 2004-06-10 2004-06-10 Houder voor het opkweken van biologisch materiaal.
NL1026379 2004-06-10
PCT/NL2005/000427 WO2005120212A1 (en) 2004-06-10 2005-06-10 Support for cultivating biological material

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2005/000427 A-371-Of-International WO2005120212A1 (en) 2004-06-10 2005-06-10 Support for cultivating biological material

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/073,053 Continuation US9095099B2 (en) 2004-06-10 2011-03-28 Container for cultivating biological materials

Publications (1)

Publication Number Publication Date
US20070292950A1 true US20070292950A1 (en) 2007-12-20

Family

ID=34970444

Family Applications (2)

Application Number Title Priority Date Filing Date
US11/570,177 Abandoned US20070292950A1 (en) 2004-06-10 2005-06-10 Support for Cultivating Biological Material
US13/073,053 Active 2025-10-04 US9095099B2 (en) 2004-06-10 2011-03-28 Container for cultivating biological materials

Family Applications After (1)

Application Number Title Priority Date Filing Date
US13/073,053 Active 2025-10-04 US9095099B2 (en) 2004-06-10 2011-03-28 Container for cultivating biological materials

Country Status (7)

Country Link
US (2) US20070292950A1 (pt)
EP (1) EP1761125B1 (pt)
DK (1) DK1761125T3 (pt)
ES (1) ES2781326T3 (pt)
NL (1) NL1026379C2 (pt)
PT (1) PT1761125T (pt)
WO (1) WO2005120212A1 (pt)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI460896B (zh) * 2011-07-21 2014-11-11 Toshiba Kk 非揮發性阻值變化元件
US20150027049A1 (en) * 2012-04-13 2015-01-29 Vivi B.V. Holder for plants and a plant cultivation method
JP2015083014A (ja) * 2009-01-14 2015-04-30 ヴィトロ・プラス・コマンディエール・フェンノートシャップ 農業用材料または生物学的材料を培養するための容器
US20180352752A1 (en) * 2016-10-14 2018-12-13 Sunshine Horticulture Co., Ltd. Method of cultivating plant in transparent sealed container and base used therefor

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL2010875C2 (nl) * 2013-05-28 2014-12-01 Wiefferink B V Container voor het bevatten van een substantie, afdekfolie voor het afdekken van een substantie in een dergelijke container en werkwijze voor het aanbrengen van een afdekfolie in een container.
CN103609455B (zh) * 2013-12-03 2016-05-25 上海交通大学 基于微扦插的非洲堇组织培养健壮苗优化生产方法
NL2012234C2 (en) 2014-02-10 2015-08-17 Vivi B V Plant holder.
US9943025B2 (en) * 2015-03-04 2018-04-17 Bloombabies Inc. Seed growing device, method, and system
CN104904594B (zh) * 2015-05-19 2018-04-10 锡山区先锋家庭农场 一种红掌花粉培养方法
US11140840B2 (en) 2015-06-02 2021-10-12 Pioneer Hi-Bred International, Inc. Plant propagation system and method
CN105594504A (zh) * 2015-12-22 2016-05-25 镇江盛弘景观植物有限公司 一种种植百合的生态有机培养土
US20180325040A1 (en) * 2017-05-09 2018-11-15 Casa Flora, Inc. Plant Material Containers and Methods

Citations (2)

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Publication number Priority date Publication date Assignee Title
US4912874A (en) * 1987-04-03 1990-04-03 Taylor Alan G Solid matrix priming of seeds
US6032409A (en) * 1995-09-05 2000-03-07 Mukoyama Orchids, Ltd. Support for cultivating plant

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ES8706011A1 (es) * 1986-01-29 1987-06-01 Alvarez Alonso Roberto Sistema para la plantacion y cultivo de plantas
US4908315A (en) * 1987-03-04 1990-03-13 Agristar, Inc. Integument and method for micropropagation and tissue culturing
GB8708441D0 (en) * 1987-04-08 1987-05-13 Hodds H Micropropagation of plants
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AU4914493A (en) * 1993-10-20 1995-05-11 Tsun-Thin Huang A bicomponent tissue and seedling culture bottle
JP2944923B2 (ja) * 1995-10-16 1999-09-06 和彦 高橋 サギソウの種子繁殖に適した栽培容器
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CA2372488C (en) * 1999-05-06 2009-01-27 Universite Laval Scalable bioreactor culture process and system for the maturation of conifer somatic embryos
US20040029266A1 (en) * 2002-08-09 2004-02-12 Emilio Barbera-Guillem Cell and tissue culture device

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
US4912874A (en) * 1987-04-03 1990-04-03 Taylor Alan G Solid matrix priming of seeds
US6032409A (en) * 1995-09-05 2000-03-07 Mukoyama Orchids, Ltd. Support for cultivating plant

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015083014A (ja) * 2009-01-14 2015-04-30 ヴィトロ・プラス・コマンディエール・フェンノートシャップ 農業用材料または生物学的材料を培養するための容器
TWI460896B (zh) * 2011-07-21 2014-11-11 Toshiba Kk 非揮發性阻值變化元件
US20150027049A1 (en) * 2012-04-13 2015-01-29 Vivi B.V. Holder for plants and a plant cultivation method
US9629314B2 (en) * 2012-04-13 2017-04-25 Vivi B.V. Holder for plants and a plant cultivation method
US20180352752A1 (en) * 2016-10-14 2018-12-13 Sunshine Horticulture Co., Ltd. Method of cultivating plant in transparent sealed container and base used therefor
US10785920B2 (en) * 2016-10-14 2020-09-29 Sunshine Horticulture Co., Ltd. Method of cultivating plant in transparent sealed container and base used therefor

Also Published As

Publication number Publication date
EP1761125B1 (en) 2020-01-08
PT1761125T (pt) 2020-04-08
US9095099B2 (en) 2015-08-04
US20110232189A1 (en) 2011-09-29
ES2781326T3 (es) 2020-09-01
EP1761125A1 (en) 2007-03-14
WO2005120212A1 (en) 2005-12-22
DK1761125T3 (da) 2020-03-30
NL1026379C2 (nl) 2005-12-14

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AS Assignment

Owner name: VISSER 'S-GRAVENDEEL HOLDING B.V., NETHERLANDS

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:BIJL, JACOB JOHANNES;REEL/FRAME:019496/0560

Effective date: 20070122

Owner name: VITRO PLUS, C.V., NETHERLANDS

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:BIJL, JACOB JOHANNES;REEL/FRAME:019496/0560

Effective date: 20070122

STCB Information on status: application discontinuation

Free format text: ABANDONED -- FAILURE TO RESPOND TO AN OFFICE ACTION