US20040052830A1 - Method for decellularising foreign material for the production of bioprostheses - Google Patents

Method for decellularising foreign material for the production of bioprostheses Download PDF

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Publication number
US20040052830A1
US20040052830A1 US10/416,697 US41669703A US2004052830A1 US 20040052830 A1 US20040052830 A1 US 20040052830A1 US 41669703 A US41669703 A US 41669703A US 2004052830 A1 US2004052830 A1 US 2004052830A1
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United States
Prior art keywords
cells
foreign material
rinsing
alcohol
flow
Prior art date
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Abandoned
Application number
US10/416,697
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English (en)
Inventor
Wolfgang Konertz
Pascal Dohmen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
AutoTissue GmbH
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Individual
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Filing date
Publication date
Application filed by Individual filed Critical Individual
Assigned to AUTOTISSUE GMBH reassignment AUTOTISSUE GMBH ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: KONERTZ, WOLFGANG, DOHMEN, PASCAL
Publication of US20040052830A1 publication Critical patent/US20040052830A1/en
Priority to US11/925,618 priority Critical patent/US7824609B2/en
Abandoned legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/24—Heart valves ; Vascular valves, e.g. venous valves; Heart implants, e.g. passive devices for improving the function of the native valve or the heart muscle; Transmyocardial revascularisation [TMR] devices; Valves implantable in the body
    • A61F2/2412—Heart valves ; Vascular valves, e.g. venous valves; Heart implants, e.g. passive devices for improving the function of the native valve or the heart muscle; Transmyocardial revascularisation [TMR] devices; Valves implantable in the body with soft flexible valve members, e.g. tissue valves shaped like natural valves
    • A61F2/2415—Manufacturing methods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
    • A61L2/0082—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using chemical substances
    • A61L2/0088—Liquid substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking

Definitions

  • the present invention relates to a method for decellularizing allogenic and xenogenic foreign material using biodetergents for the production of bioprostheses coated with endogenic cells of the recipient of the prosthesis.
  • the inventive idea is to remove foreign cells from the initial allogenic or xenogenic product to be re-coated with endogenic cells by combining a treatment with bile acid, a treatment with alcohol and upstream and downstream rinsing steps with the mechanical impact of a flowing medium on the tissue matrix and the cells to be removed at least in the last rinsing step.
  • the bile acid that is preferably used in the form of deoxycholic acid causes gradual—or with a mechanical impact, accelerated—coating of the cells with the acid to create a separating layer between the matrix made of collagen and elastin (hereinafter called ‘collagen matrix’) and the cell and to detach the cell from the matrix.
  • deoxycholic acid has a cytolytic effect.
  • the detached cells and-the deoxycholic acid are rinsed off in a subsequent rinsing step.
  • the subsequent treatment with alcohol preferably with ethanol or propanol, completely disposes of any residual deoxycholic acid as it dissolves well in alcohol.
  • the residual deoxycholic acid that may be present detaches any cells that remained in the matrix while the alcohol has a cytocidal and antiviral effect.
  • the subsequent last rinsing step is a preferably pulsating flow whose forces act upon the walls of the respective organ portion and expand the matrix but also apply a direct mechanical force onto residual cells and remove them from the expanded matrix.
  • acellular initial products i.e. organ portions such as cardiac valves or vessels that are free from any cell material and viruses for producing bioprostheses by subsequently coating these products with endogenic cells from their respective recipient.
  • the apparatus for treating an organ portion consisting of a foreign material in a flowing medium includes a decellularization chamber that receives the respective organ portion and a pump that creates the medium flow, both sequentially incorporated in a ring line.
  • the ring line includes inlet and outlet valves for feeding or draining the respective treatment medium.
  • the decellularization chamber can be detached from the ring line so that said chamber and the organ portion in the medium it contains can be moved.
  • the organ portion to be treated is fixed and preloaded in the container by sewing it to adapters shaped like the organ portion and placing it lengthwise in the direction of flow.
  • FIG. 1 shows an apparatus for decellularizing a cardiac valve in a flow circuit
  • FIG. 2 shows a sectional view of the decellularization chamber that is incorporated in the flow circuit and receives the cardiac valve
  • FIG. 3 a shows a microscopic sectional view of an aortic valve wall that has been decellularized using the method according to the invention.
  • FIG. 3 b shows a magnified view of a medial tissue section of the aortic valve wall according to FIG. 3 a.
  • a porcine aortic valve that was removed at a slaughterhouse is freed from fat, cut to size, measured, and checked for germs (fungi, aerobic and anaerobic bacteria, mycoplasma). Intermediate storage at a maximum temperature of 4° C. should not exceed seven days.
  • the cardiac valve prepared in this way is put into a 1% to 2% deoxycholic acid solution (or a bile acid with a similar effect) and stored therein for 24 hours at 37° C.
  • the deoxycholic acid is capable of forming so-called adducts with a fatty acid in the form of inclusion compounds so that the deoxycholic acid can encompass the cell on all sides, thereby dissolving its adhesive bond with the tissue matrix.
  • deoxycholic acid has a cytocidal effect.
  • a cardiac valve treated in this way is rinsed under constant motion in a dilution set of a phosphate buffer solution (PBS) at decreasing concentrations to remove the cells treated with deoxycholic acid from the tissue matrix.
  • PBS phosphate buffer solution
  • the cardiac valve is treated at room temperature for about 10 minutes in 40 per cent alcohol to produce an antiviral effect and kill any remaining cells in the collagen structure.
  • alcohol is a good solvent, it at the same times rinses off any residual acid and detaches more cells.
  • a phosphate buffer solution PBS
  • the cardiac valve is rinsed once again and then treated mechanically in a pulsating PBS media flow.
  • the pulsating media flow rhythmically widens the cardiac valve that is fixed and preloaded lengthwise to the flow in a decellularization chamber and at the same time exposed to mechanical forces.
  • This step mechanically detaches any remaining cells from the collagen structure so that an acellular structure is obtained from which all cell material has been removed and which therefore cannot contain any viruses.
  • a tissue matrix of the cardiac valve treated in this way which is free of cells and of the decellularization media used—as shown in FIG. 2—is excellently suited for re-coating with endogenic endothelial cells from the later recipient of such a bioprosthesis, and this bioprosthesis can be implanted into a human body without the risk of immunological reactions or viral influences.
  • the invention is not limited to the treatment variant described herein, both regarding the type and origin of the foreign material used for producing bioprosthesis and regarding the procedural parameters as long as the essential steps of the method, i.e. treatment with an adduct-forming bile acid and alcohol with intermediate or downstream rinsing in combination with exposure of the respective organ portion to a preferably pulsating flow for gentle mechanical action on the tissue, are executed.
  • the method can also be carried out by running not just the last rinsing step but, instead or in addition, by running other or all treatment steps in a flowing medium. This mechanically supports the effect of the respective medium, whereby better, all-area access to the cells is achieved and the cells are easier detached or removed from the expanded collagen matrix due to the action of the pulsating flow.
  • FIG. 1 An apparatus for decellularizing a cardiac valve is shown in FIG. 1. It includes a ring line 1 that incorporates a decellularization chamber 2 that receives the cardiac valve to be treated, a diaphragm pump 3 and a downstream equalizing chamber 4 .
  • the diaphragm pump 3 is connected to a drive unit (not shown) via a hose line 5 .
  • An outlet valve 6 and an inlet valve 7 whose functions approximately correspond to that of a cardiac valve are integrated into the two connections of the diaphragm pump 3 to the ring line 1 .
  • the outlet valve 6 can be omitted when treating cardiac valves as these have valve flaps.
  • the core of the apparatus is the decellularization chamber 2 for decellularizing a porcine aortic valve 8 using the additional effect of fluid force.
  • the decellularization chamber 2 consists of a transparent hollow cylinder 9 made of piacryl into the open end faces of which the teflon adapters 10 and 11 are sealingly centered and fixed, said adapters being connected to the ring line 1 via fittings 12 , 13 , each of them comprising a fixing section 14 , 15 that protrudes into the hollow cylinder 9 and has mounting holes 16 , 17 radially spaced around its periphery for firmly holding the aortic valve 8 in a preloaded state to the rims of the end faces.
  • the outer diameter of the two fixing sections 14 , 15 of the adapters 10 , 11 approximately is the same as the diameter of the aortic valve 8 .
  • the rear adapter 11 can be braced via a bridge 18 and a first packing 27 on the inside of a ring land 20 that is connected to the hollow cylinder 9 by turning a threaded ring 21 whose female thread engages in a male thread on the adapter 11 .
  • the adapter 10 comprises a collar 22 that rests against the end surface of the hollow cylinder 9 and can be braced using a threaded cap 23 with a female thread that engages in a male thread on the hollow cylinder 9 .
  • a second packing 19 is provided for leak proof mounting.
  • the hose piece of the ring line 1 that is topped by the decellularization chamber is made of a flexible material (silicone) to ensure flow-through due to the pulsating pumping effect.
  • a suitably prepared aortic valve 8 can be sewed outside the hollow cylinder 9 to the opposite fixing sections 14 , 15 of the adapters 10 , 11 .
  • the aortic valve 8 fixed as described above, is inserted into the hollow cylinder 9 .
  • the deoxycholic acid is introduced into the decellularization chamber 2 and the ring line 1 via an inlet and outlet valve 24 , 25 in the ring line 1 or one of the fittings 13 , 14 ; then, the diaphragm pump 3 is activated so that a pulsating flow of deoxycholic acid continuously flows by or through the aortic valve 8 , and the mechanical force this flow exerts on the tissue completes the detachment and removal of cells that are foreign to the recipient of the cardiac valve.
  • Physiological saline or phosphate buffer solution is filled into the apparatus after discharging the deoxycholic acid, and the tissue is rinsed until all the deoxycholic acids and any toxic constituents are removed. After this rinsing step, treatment of the aortic valve 8 with alcohol and another rinsing step in phosphate buffer solution follow.
  • All treatment steps of the decellularization method take place in the apparatus described above in a pulsating flow of the respective medium.
  • the direction of flow is the natural flow direction when the bioprosthesis is implanted.
  • the inlet and outlet valves 24 , 25 are used for media replacement, however fresh rinsing solution can be supplied, and used rinsing solution can be discharged, continuously for the rinsing step.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Cardiology (AREA)
  • Botany (AREA)
  • Dermatology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Manufacturing & Machinery (AREA)
  • General Chemical & Material Sciences (AREA)
  • Materials For Medical Uses (AREA)
  • Prostheses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
US10/416,697 2000-12-20 2001-12-05 Method for decellularising foreign material for the production of bioprostheses Abandoned US20040052830A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US11/925,618 US7824609B2 (en) 2000-12-20 2007-10-26 Method for decellularizing foreign material to produce bioprostheses

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10064948.3 2000-12-20
DE10064948A DE10064948C1 (de) 2000-12-20 2000-12-20 Verfahren zur Dezellularisierung von Fremdmaterial zur Herstellung von Bioprothesen und Vorrichtung zur Durchführung des Verfahrens
PCT/DE2001/004616 WO2002049681A1 (de) 2000-12-20 2001-12-05 Verfahren zur dezellularisierung von fremdmaterial zur herstellung von bioprothesen

Related Child Applications (1)

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US11/925,618 Continuation US7824609B2 (en) 2000-12-20 2007-10-26 Method for decellularizing foreign material to produce bioprostheses

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US20040052830A1 true US20040052830A1 (en) 2004-03-18

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US10/416,697 Abandoned US20040052830A1 (en) 2000-12-20 2001-12-05 Method for decellularising foreign material for the production of bioprostheses
US11/925,618 Expired - Fee Related US7824609B2 (en) 2000-12-20 2007-10-26 Method for decellularizing foreign material to produce bioprostheses

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US (2) US20040052830A1 (de)
EP (1) EP1343536B1 (de)
JP (1) JP2004531291A (de)
KR (1) KR100650223B1 (de)
CN (1) CN1256987C (de)
AT (1) ATE306284T1 (de)
AU (2) AU2002219008B2 (de)
BR (1) BR0116309A (de)
CA (1) CA2428880C (de)
DE (3) DE10064948C1 (de)
DK (1) DK1343536T3 (de)
ES (1) ES2249385T3 (de)
IL (2) IL155689A0 (de)
MX (1) MXPA03005597A (de)
NZ (1) NZ525626A (de)
RU (1) RU2281120C2 (de)
WO (1) WO2002049681A1 (de)
ZA (1) ZA200303665B (de)

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US20060212074A1 (en) * 2003-05-15 2006-09-21 Waseda University Method of removing cell
US20070010897A1 (en) * 2005-01-06 2007-01-11 Stone Kevin R Immunochemically modified and sterilized xenografts and allografts
CN100372511C (zh) * 2005-11-30 2008-03-05 烟台正海生物技术有限公司 一种脱细胞真皮基质
US20080195229A1 (en) * 2007-02-09 2008-08-14 Quijano Rodolfo C Decellularized pericardial tissue
US20080195230A1 (en) * 2007-02-09 2008-08-14 Quijano Rodolfo C Pericardial tissue sheet
WO2008125851A2 (en) * 2007-04-16 2008-10-23 Tissue Science Laboratories Plc Vascular implant
WO2009085547A2 (en) 2007-12-19 2009-07-09 Ethicon, Inc. Decellularized omentum matrix and uses thereof
US20100191346A1 (en) * 2007-04-16 2010-07-29 Stephen Bloor Bone implant
US8753406B2 (en) 2010-08-31 2014-06-17 Zimmer Inc. Osteochondral graft delivery device and uses thereof
US20160022408A1 (en) * 2014-07-23 2016-01-28 Clemson University Decellularization Method and System and Decellularized Tissue Formed Thereby
US10092600B2 (en) 2013-07-30 2018-10-09 Musculoskeletal Transplant Foundation Method of preparing an adipose tissue derived matrix
US10912864B2 (en) 2015-07-24 2021-02-09 Musculoskeletal Transplant Foundation Acellular soft tissue-derived matrices and methods for preparing same
US11052175B2 (en) 2015-08-19 2021-07-06 Musculoskeletal Transplant Foundation Cartilage-derived implants and methods of making and using same
WO2021241880A1 (ko) * 2020-05-29 2021-12-02 주식회사 로킷헬스케어 그물막을 이용한 신장 치료용 조성물, 이를 포함하는 신장 치료용 의료 키트, 및 이의 경화물을 포함하는 신장 치료용 필름

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DE10138564B4 (de) * 2001-08-06 2007-07-12 Lamm, Peter Wilhelm, Dr. Verfahren zur Devitalisierung natürlicher Organe und/oder zur Bereitstellung extrazellulärer Matrices zum "Tissue-Engineering"
US6761735B2 (en) * 2002-04-25 2004-07-13 Medtronic, Inc. Heart valve fixation process and apparatus
JP4092397B2 (ja) 2002-09-10 2008-05-28 国立循環器病センター総長 超高静水圧印加による移植用生体組織の処理方法
DE10258121B3 (de) * 2002-12-06 2004-03-18 Auto Tissue Gmbh Verfahren zur Herstellung von Bioprothesen
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CA2618731C (en) 2005-08-26 2021-12-28 Regents Of The University Of Minnesota Decellularization and recellularization of organs and tissues
US20100093066A1 (en) * 2005-08-26 2010-04-15 Regents Of The University Of Minnesota Decellularization and recellularization apparatuses and systems containing the same
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US8579964B2 (en) 2010-05-05 2013-11-12 Neovasc Inc. Transcatheter mitral valve prosthesis
JP5931878B2 (ja) 2010-09-01 2016-06-08 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ 移植可能性の改良のための組織または器官の再細胞化法
US9308087B2 (en) 2011-04-28 2016-04-12 Neovasc Tiara Inc. Sequentially deployed transcatheter mitral valve prosthesis
US9554897B2 (en) 2011-04-28 2017-01-31 Neovasc Tiara Inc. Methods and apparatus for engaging a valve prosthesis with tissue
US9345573B2 (en) 2012-05-30 2016-05-24 Neovasc Tiara Inc. Methods and apparatus for loading a prosthesis onto a delivery system
US9290738B2 (en) 2012-06-13 2016-03-22 Miromatrix Medical Inc. Methods of decellularizing bone
KR102278652B1 (ko) 2013-03-15 2021-07-19 미로매트릭스 메디칼 인크. 섬 세포 재세포화를 위한 관류 탈세포화된 간의 용도
US9572665B2 (en) 2013-04-04 2017-02-21 Neovasc Tiara Inc. Methods and apparatus for delivering a prosthetic valve to a beating heart
US10537662B2 (en) 2013-07-31 2020-01-21 Biotronik Ag Method for preparing biological tissue
EP2832379B1 (de) * 2013-07-31 2015-07-22 Biotronik AG Verfahren zur Aufbereitung von biologischem Gewebe
EP2918294A1 (de) * 2014-03-11 2015-09-16 Bayer Technology Services GmbH Vorrichtung und Verfahren zur kontinuierlichen Virusinaktivierung
CN105169481B (zh) * 2015-09-29 2018-05-18 陕西瑞盛生物科技有限公司 一种生物材料的脱细胞方法
US10433952B2 (en) 2016-01-29 2019-10-08 Neovasc Tiara Inc. Prosthetic valve for avoiding obstruction of outflow
CN105770991B (zh) * 2016-03-03 2018-09-25 曾祥军 生物源性静脉瓣膜的制备方法
CA3027505A1 (en) 2016-06-15 2017-12-21 The General Hospital Corporation Metabolic labeling and molecular enhancement of biological materials using bioorthogonal reactions
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