IT1282207B1 - Sistemi di coltura di cellule staminali di midollo osseo umano in matrici tridimensionali costituiti da esteri dell'acido ialuronico - Google Patents

Sistemi di coltura di cellule staminali di midollo osseo umano in matrici tridimensionali costituiti da esteri dell'acido ialuronico

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Publication number
IT1282207B1
IT1282207B1 IT95PD000225A ITPD950225A IT1282207B1 IT 1282207 B1 IT1282207 B1 IT 1282207B1 IT 95PD000225 A IT95PD000225 A IT 95PD000225A IT PD950225 A ITPD950225 A IT PD950225A IT 1282207 B1 IT1282207 B1 IT 1282207B1
Authority
IT
Italy
Prior art keywords
bone marrow
hyaluronic acid
marrow stem
connective tissue
cell culture
Prior art date
Application number
IT95PD000225A
Other languages
English (en)
Inventor
Giovanni Abatangelo
Lanfranco Callegaro
Original Assignee
Fidia Advanced Biopolymers Srl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fidia Advanced Biopolymers Srl filed Critical Fidia Advanced Biopolymers Srl
Publication of ITPD950225A0 publication Critical patent/ITPD950225A0/it
Priority to IT95PD000225A priority Critical patent/IT1282207B1/it
Priority to AU76934/96A priority patent/AU709236B2/en
Priority to US09/041,287 priority patent/US6596274B1/en
Priority to PCT/EP1996/005093 priority patent/WO1997018842A1/en
Priority to AT96939845T priority patent/ATE231730T1/de
Priority to IL12445096A priority patent/IL124450A/en
Priority to EP96939845A priority patent/EP0863776B1/en
Priority to JP51938597A priority patent/JP3808900B2/ja
Priority to CA002238011A priority patent/CA2238011C/en
Priority to DK96939845T priority patent/DK0863776T3/da
Priority to PT96939845T priority patent/PT863776E/pt
Priority to DE69626035T priority patent/DE69626035T2/de
Priority to ES96939845T priority patent/ES2189890T3/es
Publication of ITPD950225A1 publication Critical patent/ITPD950225A1/it
Application granted granted Critical
Publication of IT1282207B1 publication Critical patent/IT1282207B1/it
Priority to US09/602,033 priority patent/US6482231B1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
    • A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
    • A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
    • A61L15/28—Polysaccharides or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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    • A—HUMAN NECESSITIES
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
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    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
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    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin
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    • C12N5/0068—General culture methods using substrates
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0655—Chondrocytes; Cartilage
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    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
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    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
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    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
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IT95PD000225A 1995-11-20 1995-11-20 Sistemi di coltura di cellule staminali di midollo osseo umano in matrici tridimensionali costituiti da esteri dell'acido ialuronico IT1282207B1 (it)

Priority Applications (14)

Application Number Priority Date Filing Date Title
IT95PD000225A IT1282207B1 (it) 1995-11-20 1995-11-20 Sistemi di coltura di cellule staminali di midollo osseo umano in matrici tridimensionali costituiti da esteri dell'acido ialuronico
EP96939845A EP0863776B1 (en) 1995-11-20 1996-11-19 A biologic material comprising an efficient culture of bone marrow stem cells partially or completely differentiated into connective tissue cells and a three-dimensional biocompatible and biodegradable matrix consisting of a hyaluronic acid derivative
CA002238011A CA2238011C (en) 1995-11-20 1996-11-19 A biologic material comprising an efficient culture of bone marrow stem cells partially or completely differentiated into connective tissue cells and a three-dimensional biocompatible and biodegradable matrix consisting of a hyaluronic acid derivative
PCT/EP1996/005093 WO1997018842A1 (en) 1995-11-20 1996-11-19 A biologic material comprising an efficient culture of bone marrow stem cells partially or completely differentiated into connective tissue cells and a three-dimensional biocompatible and biodegradable matrix consisting of a hyaluronic acid derivative
AT96939845T ATE231730T1 (de) 1995-11-20 1996-11-19 Ein biologisches material, das eine wirksame kultur von knochenmarkstammzellen, die teilweise oder vollständing in bindegewebszellen differenziert sind, enthält, und eine dreidimensionale, bioverträgliche und biologische abbaubare matrix, die aus einen hyaluronsäurederivat besteht
IL12445096A IL124450A (en) 1995-11-20 1996-11-19 Biological material comprising an efficient culture of bone marrow stem cells partially or completely differentiated into connective tissue cells and a three-dimensional biocompatible and biodegradable matrix consisting of a hyaluronic acid derivative
AU76934/96A AU709236B2 (en) 1995-11-20 1996-11-19 A biologic material comprising an efficient culture of bone marrow stem cells partially or completely differentiated into connective tissue cells and a three-dimensional biocompatible and biodegradable matrix consisting of hyaluronic acid derivative
JP51938597A JP3808900B2 (ja) 1995-11-20 1996-11-19 結合組織細胞に部分的又は完全に分化した骨髄幹細胞の有効培養物及びヒアルロン酸誘導体より成る三次元の生体親和性で且つ生分解性のマトリックスから構成される生物学的物質
US09/041,287 US6596274B1 (en) 1995-11-20 1996-11-19 Biological material containing bone marrow stem cells partially or completely differentiated into connective tissue cells and a hyaluronic acid ester matrix
DK96939845T DK0863776T3 (da) 1995-11-20 1996-11-19 Et biologisk materiale, der omfatter en effektiv kultur af knoglemarvsstamceller, der er differentieret delvist eller fuldstændigt til bindevævsceller og en tredimensionel biokompatibel og biologisk nedbrydelig matrix bestående af en hyaluronsyrederivat
PT96939845T PT863776E (pt) 1995-11-20 1996-11-19 Material biologico compreendendo uma cultura eficiente de celulas estaminais de medula ossea parcialmente ou completamente diferenciadas em celulas detecido conjuntivo e uma matriz tridimensional biocompativel e biodegradavel consistindo num derivado de acido hialuronico
DE69626035T DE69626035T2 (de) 1995-11-20 1996-11-19 Ein biologisches material, das eine wirksame kultur von knochenmarkstammzellen, die teilweise oder vollständing in bindegewebszellen differenziert sind, enthält, und eine dreidimensionale, bioverträgliche und biologische abbaubare matrix, die aus einen hyaluronsäurederivat besteht
ES96939845T ES2189890T3 (es) 1995-11-20 1996-11-19 Un material biologico comprendiendo un cultivo eficaz de celulas madre de medula osea parcialmente o complemtamente diferenciadas en celulas de tejido conectivo y una matriz tridimensional biocompatible y biodegradable consistente en un derivado del acido hialuronico.
US09/602,033 US6482231B1 (en) 1995-11-20 2000-06-23 Biological material for the repair of connective tissue defects comprising mesenchymal stem cells and hyaluronic acid derivative

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT95PD000225A IT1282207B1 (it) 1995-11-20 1995-11-20 Sistemi di coltura di cellule staminali di midollo osseo umano in matrici tridimensionali costituiti da esteri dell'acido ialuronico

Publications (3)

Publication Number Publication Date
ITPD950225A0 ITPD950225A0 (it) 1995-11-20
ITPD950225A1 ITPD950225A1 (it) 1997-05-20
IT1282207B1 true IT1282207B1 (it) 1998-03-16

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Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5594651A (en) 1995-02-14 1997-01-14 St. Ville; James A. Method and apparatus for manufacturing objects having optimized response characteristics
US6152964A (en) * 1996-03-01 2000-11-28 Isotis B.V. Method for in vitro production of bone
BR9815713A (pt) * 1997-02-20 2002-11-05 Gerigene Medical Corp Método para aumento e reparo de defeitos de tecido dérmico, subcutâneo e das cordas vocais
US7767452B2 (en) 1997-02-20 2010-08-03 Kleinsek Don A Tissue treatments with adipocyte cells
IT1293484B1 (it) * 1997-06-11 1999-03-01 Fidia Advanced Biopolymers Srl Materiale biologico comprendente una efficiente coltura di cellule e una matrice tridimensionale biocompatibile e biodegradabile
FR2771102B1 (fr) 1997-11-14 2000-12-15 Biocoral Inc Procede pour induire la differenciation osteoblastique de cellules de tissu adipeux extramedullaire humain
WO1999031222A1 (en) * 1997-12-18 1999-06-24 Willem Frederik Van Eelen Industrial scale production of meat from in vitro cell cultures
US20030147860A1 (en) 2002-02-07 2003-08-07 Marchosky J. Alexander Compositions and methods for forming and strengthening bone
US6872819B1 (en) 1998-05-27 2005-03-29 Fidia Advanced Biopolymers S.R.L. Biomaterials containing hyaluronic acid derivatives in the form of three-dimensional structures free from cellular components or products thereof for the in vivo regeneration of tissue cells
ITPD980149A1 (it) * 1998-06-17 1999-12-17 Fidia Advanced Biopolymers Srl Protesi tridimensionali comprendenti derivati dell'acido ialuronico per riparare o ricostruire i tessuti danneggiati e processo per la
ITPD980168A1 (it) * 1998-07-06 2000-01-06 Fidia Advanced Biopolymers Srl Composizioni biocompatibili e biodegradabili comprendenti acido ialuronico e suoi derivati per il trattamento delle ulcere dell'appara
DE19855890A1 (de) 1998-12-03 2000-06-08 Nerlich Michael Poröse Kompositmatrix, deren Herstellung und Verwendung
CA2377435A1 (en) 1999-06-29 2001-01-04 J. Alexander Marchosky Compositions and methods for forming and strengthening bone
RU2305864C2 (ru) * 1999-08-23 2007-09-10 ВИЛЛЕ Джеймс А. СТ. Система и способ изготовления
DE60009956T2 (de) * 1999-12-28 2005-04-28 Iso Tis N.V. Wachstumsfaktoren und l-glutamin enthaltendes zellkulturmedium
JP3848164B2 (ja) * 2000-05-03 2006-11-22 フィディア・アドバンスト・バイオポリマーズ・ソシエタ・ア・レスポンサビリタ・リミタータ 軟部組織修復用の前脂肪細胞からなるバイオマテリアル
DE10064948C1 (de) * 2000-12-20 2002-07-11 Auto Tissue Gmbh Verfahren zur Dezellularisierung von Fremdmaterial zur Herstellung von Bioprothesen und Vorrichtung zur Durchführung des Verfahrens
WO2003029418A2 (en) * 2001-10-02 2003-04-10 Becton, Dickinson And Company Proliferation and differentiation of stem cells using extracellular matrix and other molecules
DE10203644A1 (de) * 2002-01-30 2003-08-07 Fraunhofer Ges Forschung Kryokonservierung an textilen Geweben
US7326571B2 (en) 2003-07-17 2008-02-05 Boston Scientific Scimed, Inc. Decellularized bone marrow extracellular matrix
US7524514B2 (en) * 2003-12-01 2009-04-28 Tissue Engineering Consultants, Inc. Biomimetic composition reinforced by a polyelectrolytic complex of hyaluronic acid and chitosan
US20070190101A1 (en) * 2004-03-31 2007-08-16 Chunlin Yang Flowable bone grafts
JP4949241B2 (ja) * 2004-07-12 2012-06-06 イスト・テクノロジーズ・インコーポレイテッド 組織マトリックスシステム
US8512730B2 (en) * 2004-07-12 2013-08-20 Isto Technologies, Inc. Methods of tissue repair and compositions therefor
US20060035290A1 (en) * 2004-08-13 2006-02-16 Medtronic, Inc. Isolation of endothelial progenitor cell subsets and methods for their use
US7439057B2 (en) * 2004-11-16 2008-10-21 La Jolla Bioengineering Institute Convective flow tissue assembly
JP2009513207A (ja) * 2005-10-27 2009-04-02 コロプラスト アクティーゼルスカブ Ｅｃｍ材料を伴う生分解性足場
WO2007064305A1 (en) * 2005-12-01 2007-06-07 Agency For Science, Technology And Research Three-dimensional reconstituted extracellular matrices as scaffolds for tissue engineering
CA2631520A1 (en) * 2005-12-07 2007-06-14 Isto Technologies, Inc. Cartilage repair methods
US9572886B2 (en) 2005-12-22 2017-02-21 Nitto Denko Corporation Agent for treating myelofibrosis
JP5342834B2 (ja) * 2008-09-05 2013-11-13 日東電工株式会社 骨髄線維症処置剤
ITTO20060282A1 (it) * 2006-04-14 2007-10-15 Univ Degli Studi Torino Mezzo di coltura e composizione farmaceutica per la rigenerazione del tessuto cartilagineo relativo procedimento relativi usi e prodotti
DE102007005946A1 (de) * 2007-02-01 2008-08-14 Stiftung Caesar Therapeutische Zusammensetzung und Verwendung einer zellfreien Substanz
EP2155860B1 (en) * 2007-05-03 2014-08-27 The Brigham and Women's Hospital, Inc. Multipotent stem cells and uses thereof
US8574567B2 (en) * 2007-05-03 2013-11-05 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
WO2008156220A1 (ja) 2007-06-20 2008-12-24 Hoya Corporation 肥大化能を有する軟骨細胞の産生する因子によって誘導された細胞と足場による骨欠損の修復と治療
KR20150021587A (ko) * 2007-08-06 2015-03-02 메소블라스트, 아이엔씨. 생체 내에서 결합 조직을 생성, 복구 및/또는 유지하는 방법
EP3095470A1 (en) 2008-02-07 2016-11-23 Shahar Cohen Compartmental extract compositions for tissue engineering
SG10201507149RA (en) 2008-06-25 2015-10-29 Mesoblast Inc Repair and/or Reconstitution of Invertebral Discs
US20100055078A1 (en) * 2008-08-15 2010-03-04 The Government of the U.S.A, as represented by the Department of Veterans Affairs Methods of making a transplantable bone repair system using multipotent stem cells
WO2010146177A1 (en) * 2009-06-18 2010-12-23 Mc2 Cell Aps Bone marrow extracellular matrix extract and therapeutic use thereof
IT1394570B1 (it) 2009-07-02 2012-07-05 Fidia Farmaceutici Materiale biologico adatto per la terapia dell osteoartrosi del danno dei legamenti e per il trattamento delle patologie delle articolazioni.
EP2533859B1 (en) 2010-02-10 2016-04-06 Nayacure Therapeutics Ltd Pharmaceutical compositions for the treatment and prevention of cancer
US10130736B1 (en) 2010-05-14 2018-11-20 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US8883210B1 (en) 2010-05-14 2014-11-11 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US9352003B1 (en) 2010-05-14 2016-05-31 Musculoskeletal Transplant Foundation Tissue-derived tissuegenic implants, and methods of fabricating and using same
US8895291B2 (en) 2010-10-08 2014-11-25 Terumo Bct, Inc. Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions
WO2012142569A2 (en) 2011-04-15 2012-10-18 The Regents Of The University Of California Decellularized extracellular matrix
US8834928B1 (en) 2011-05-16 2014-09-16 Musculoskeletal Transplant Foundation Tissue-derived tissugenic implants, and methods of fabricating and using same
US9295756B2 (en) 2012-02-01 2016-03-29 Nayacure Therapeutics Ltd. Methods for inducing immune tolerance to organ transplants
US10245306B2 (en) 2012-11-16 2019-04-02 Isto Technologies Ii, Llc Flexible tissue matrix and methods for joint repair
US20150037436A1 (en) 2013-07-30 2015-02-05 Musculoskeletal Transplant Foundation Acellular soft tissue-derived matrices and methods for preparing same
EP3052083A4 (en) 2013-09-30 2017-05-03 Bioactive Regenerative Therapeutics, Inc. Biomimetic hybrid gel compositions and methods of use
CN105992816B (zh) 2013-11-16 2018-04-17 泰尔茂比司特公司 生物反应器中的细胞扩增
JP6783143B2 (ja) 2014-03-25 2020-11-11 テルモ ビーシーティー、インコーポレーテッド 培地の受動的補充
WO2016049421A1 (en) 2014-09-26 2016-03-31 Terumo Bct, Inc. Scheduled feed
US10179191B2 (en) 2014-10-09 2019-01-15 Isto Technologies Ii, Llc Flexible tissue matrix and methods for joint repair
EP3297694A1 (en) 2015-05-21 2018-03-28 Musculoskeletal Transplant Foundation Modified demineralized cortical bone fibers
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US10912864B2 (en) 2015-07-24 2021-02-09 Musculoskeletal Transplant Foundation Acellular soft tissue-derived matrices and methods for preparing same
WO2017019822A1 (en) * 2015-07-29 2017-02-02 Medivation Technologies, Inc. Pellet composition containing repair cells
US11052175B2 (en) 2015-08-19 2021-07-06 Musculoskeletal Transplant Foundation Cartilage-derived implants and methods of making and using same
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
EP3656842A1 (en) 2017-03-31 2020-05-27 Terumo BCT, Inc. Cell expansion

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5510254A (en) * 1986-04-18 1996-04-23 Advanced Tissue Sciences, Inc. Three dimensional cell and tissue culture system
IT1198449B (it) * 1986-10-13 1988-12-21 F I D I Farmaceutici Italiani Esteri di alcoli polivalenti di acido ialuronico
IT1248934B (it) * 1990-06-01 1995-02-11 Fidia Spa Membrane forate biocompatibili,processi per la loro preparazione,loro impiego come supporto per la crescita in vitro di cellule epiteliali, pelli artificiali cosi' ottenute e loro impiego nei trapianti di pelle
US5197985A (en) * 1990-11-16 1993-03-30 Caplan Arnold I Method for enhancing the implantation and differentiation of marrow-derived mesenchymal cells
IT1251151B (it) * 1991-08-05 1995-05-04 Fidia Spa Materiale spugnoso essenzialmente costituito da acido ialuronico,o suoi derivati
IT1254704B (it) * 1991-12-18 1995-10-09 Mini Ricerca Scient Tecnolog Tessuto non tessuto essenzialmente costituito da derivati dell'acido ialuronico
IT1281886B1 (it) * 1995-05-22 1998-03-03 Fidia Advanced Biopolymers Srl Processo per la preparazione di idrogel ottenuti da derivati chimici dell'acido ialuronico mediante irradiazioni ultraviolette e loro
US5776193A (en) * 1995-10-16 1998-07-07 Orquest, Inc. Bone grafting matrix

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WO1997018842A1 (en) 1997-05-29
JP3808900B2 (ja) 2006-08-16
ATE231730T1 (de) 2003-02-15
DE69626035T2 (de) 2004-01-22
CA2238011C (en) 2007-01-09
IL124450A (en) 2004-02-19
DE69626035D1 (de) 2003-03-06
JP2000500372A (ja) 2000-01-18
US6596274B1 (en) 2003-07-22
AU7693496A (en) 1997-06-11
DK0863776T3 (da) 2003-05-26
CA2238011A1 (en) 1997-05-29
IL124450A0 (en) 1998-12-06
ITPD950225A0 (it) 1995-11-20
PT863776E (pt) 2003-06-30
EP0863776B1 (en) 2003-01-29
AU709236B2 (en) 1999-08-26
ES2189890T3 (es) 2003-07-16
EP0863776A1 (en) 1998-09-16

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