US11980186B2 - Preparation method of eucalyptol emulsion and application thereof in biopesticides - Google Patents
Preparation method of eucalyptol emulsion and application thereof in biopesticides Download PDFInfo
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- US11980186B2 US11980186B2 US18/155,264 US202318155264A US11980186B2 US 11980186 B2 US11980186 B2 US 11980186B2 US 202318155264 A US202318155264 A US 202318155264A US 11980186 B2 US11980186 B2 US 11980186B2
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- United States
- Prior art keywords
- eucalyptol
- emulsion
- cyclodextrin
- emulsifier
- stable
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- WEEGYLXZBRQIMU-UHFFFAOYSA-N 1,8-cineole Natural products C1CC2CCC1(C)OC2(C)C WEEGYLXZBRQIMU-UHFFFAOYSA-N 0.000 title claims abstract description 84
- 229960005233 cineole Drugs 0.000 title claims abstract description 82
- WEEGYLXZBRQIMU-WAAGHKOSSA-N Eucalyptol Chemical compound C1C[C@H]2CC[C@]1(C)OC2(C)C WEEGYLXZBRQIMU-WAAGHKOSSA-N 0.000 title claims abstract description 81
- 239000000839 emulsion Substances 0.000 title claims abstract description 77
- 238000002360 preparation method Methods 0.000 title abstract description 10
- 230000000853 biopesticidal effect Effects 0.000 title abstract description 9
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract description 44
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 43
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 26
- 239000000243 solution Substances 0.000 claims abstract description 15
- 239000007957 coemulsifier Substances 0.000 claims abstract description 13
- 239000011259 mixed solution Substances 0.000 claims abstract description 12
- 238000002156 mixing Methods 0.000 claims abstract description 9
- 230000001804 emulsifying effect Effects 0.000 claims abstract description 6
- 239000000126 substance Substances 0.000 claims abstract description 6
- -1 polyoxyethylene Polymers 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 8
- 239000004359 castor oil Substances 0.000 claims description 6
- 235000019438 castor oil Nutrition 0.000 claims description 6
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 claims description 5
- 229920001450 Alpha-Cyclodextrin Polymers 0.000 claims description 4
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 4
- HFHDHCJBZVLPGP-RWMJIURBSA-N alpha-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO HFHDHCJBZVLPGP-RWMJIURBSA-N 0.000 claims description 4
- 229940043377 alpha-cyclodextrin Drugs 0.000 claims description 4
- GDSRMADSINPKSL-HSEONFRVSA-N gamma-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO GDSRMADSINPKSL-HSEONFRVSA-N 0.000 claims description 4
- 229940080345 gamma-cyclodextrin Drugs 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 claims description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 4
- 229920000053 polysorbate 80 Polymers 0.000 claims description 4
- 239000001116 FEMA 4028 Substances 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 3
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 3
- 229960004853 betadex Drugs 0.000 claims description 3
- 229950011392 sorbitan stearate Drugs 0.000 claims 3
- 238000013517 stratification Methods 0.000 abstract description 9
- 230000000749 insecticidal effect Effects 0.000 abstract description 7
- 239000012895 dilution Substances 0.000 description 16
- 238000010790 dilution Methods 0.000 description 16
- 229920000136 polysorbate Polymers 0.000 description 15
- 238000012360 testing method Methods 0.000 description 15
- 239000002245 particle Substances 0.000 description 13
- 241001124076 Aphididae Species 0.000 description 9
- 239000007788 liquid Substances 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 241000238631 Hexapoda Species 0.000 description 7
- 241000254127 Bemisia tabaci Species 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 4
- 238000004945 emulsification Methods 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- 230000001018 virulence Effects 0.000 description 4
- BACYUWVYYTXETD-UHFFFAOYSA-N N-Lauroylsarcosine Chemical compound CCCCCCCCCCCC(=O)N(C)CC(O)=O BACYUWVYYTXETD-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000010642 eucalyptus oil Substances 0.000 description 3
- 229940044949 eucalyptus oil Drugs 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000002917 insecticide Substances 0.000 description 3
- 108700004121 sarkosyl Proteins 0.000 description 3
- KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 241000751139 Beauveria bassiana Species 0.000 description 2
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 description 2
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 229930014456 matrine Natural products 0.000 description 2
- 235000020354 squash Nutrition 0.000 description 2
- 125000002006 1,8-cineol group Chemical group 0.000 description 1
- 239000005878 Azadirachtin Substances 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 241001094807 Ericaphis scammelli Species 0.000 description 1
- 244000166124 Eucalyptus globulus Species 0.000 description 1
- 244000062241 Kaempferia galanga Species 0.000 description 1
- 235000013421 Kaempferia galanga Nutrition 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 235000004443 Ricinus communis Nutrition 0.000 description 1
- 244000178231 Rosmarinus officinalis Species 0.000 description 1
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 description 1
- 244000128884 Zier Kohl Species 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- VEHPJKVTJQSSKL-UHFFFAOYSA-N azadirachtin Natural products O1C2(C)C(C3(C=COC3O3)O)CC3C21C1(C)C(O)C(OCC2(OC(C)=O)C(CC3OC(=O)C(C)=CC)OC(C)=O)C2C32COC(C(=O)OC)(O)C12 VEHPJKVTJQSSKL-UHFFFAOYSA-N 0.000 description 1
- FTNJWQUOZFUQQJ-IRYYUVNJSA-N azadirachtin A Natural products C([C@@H]([C@]1(C=CO[C@H]1O1)O)[C@]2(C)O3)[C@H]1[C@]23[C@]1(C)[C@H](O)[C@H](OC[C@@]2([C@@H](C[C@@H]3OC(=O)C(\C)=C/C)OC(C)=O)C(=O)OC)[C@@H]2[C@]32CO[C@@](C(=O)OC)(O)[C@@H]12 FTNJWQUOZFUQQJ-IRYYUVNJSA-N 0.000 description 1
- FTNJWQUOZFUQQJ-NDAWSKJSSA-N azadirachtin A Chemical compound C([C@@H]([C@]1(C=CO[C@H]1O1)O)[C@]2(C)O3)[C@H]1[C@]23[C@]1(C)[C@H](O)[C@H](OC[C@@]2([C@@H](C[C@@H]3OC(=O)C(\C)=C\C)OC(C)=O)C(=O)OC)[C@@H]2[C@]32CO[C@@](C(=O)OC)(O)[C@@H]12 FTNJWQUOZFUQQJ-NDAWSKJSSA-N 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229940112822 chewing gum Drugs 0.000 description 1
- 235000015218 chewing gum Nutrition 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
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- 230000001186 cumulative effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
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- 239000003085 diluting agent Substances 0.000 description 1
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- 229940079593 drug Drugs 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000004530 micro-emulsion Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 229930003658 monoterpene Natural products 0.000 description 1
- 239000007764 o/w emulsion Substances 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 238000003921 particle size analysis Methods 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229950008882 polysorbate Drugs 0.000 description 1
- 229940068968 polysorbate 80 Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229940080817 rotenone Drugs 0.000 description 1
- JUVIOZPCNVVQFO-UHFFFAOYSA-N rotenone Natural products O1C2=C3CC(C(C)=C)OC3=CC=C2C(=O)C2C1COC1=C2C=C(OC)C(OC)=C1 JUVIOZPCNVVQFO-UHFFFAOYSA-N 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000002226 simultaneous effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000001587 sorbitan monostearate Substances 0.000 description 1
- 235000011076 sorbitan monostearate Nutrition 0.000 description 1
- 229940035048 sorbitan monostearate Drugs 0.000 description 1
- 229950004959 sorbitan oleate Drugs 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 229940034610 toothpaste Drugs 0.000 description 1
- 239000000606 toothpaste Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- the present disclosure relates to a preparation method of eucalyptol emulsion and application thereof in biopesticides, belonging to the field of emulsion preparation.
- Eucalyptol also known as 1,8-cineole, is a monoterpenoid compound. It mainly exists in the volatile oils of plants such as eucalyptus leaves, rosemary, and galangal. It has antibacterial, anti-inflammatory, insecticidal, analgesic and other effects. Therefore, it is widely used in medicine, daily chemicals, food and other industries. Eucalyptol can be used in combination with other drugs for the treatment of influenza, enteritis and various infections. It can also be used as a spice to prepare perfume, detergent, toothpaste and other products. In addition, the eucalyptol has a cool aroma and can be used as a food additive in chewing gum. In summary, the eucalyptol is rich in sources and has a wide range of uses, thus having high economic value and development prospects.
- the eucalyptol is almost insoluble in water, volatile and poor in stability, which limits the application of the eucalyptol in industrial conditions such as light, heat and air.
- the hydrophobicity of the eucalyptol also limits its effectiveness in an aqueous environment.
- the eucalyptol is not fully compatible with the internal environments of insects, and it is difficult for the eucalyptol to reach the target point, which weakens its effectiveness.
- Eucalyptol is almost insoluble in water, volatile and poor in stability. Therefore, the prepared aqueous solution has the problems of uneven dispersion and poor stability.
- the present disclosure uses an emulsifier combined with a co-emulsifier to prepare eucalyptol emulsion, which greatly improves the water solubility of the eucalyptol. Furthermore, cyclodextrin is used to encapsulate the eucalyptol, which has more advantages in improving the physical and chemical properties of the eucalyptol, preventing volatilization, enhancing solubility, reducing its sensitivity to the environment, and the like.
- step (3) adding the mixed solution of the emulsifier and the co-emulsifier obtained in step (2) and water into the mixed solution obtained in step (1), and emulsifying to obtain the eucalyptol emulsion.
- the mass percents of substances from steps (1), (2) and (3) are as follows: 0.5%-1% of cyclodextrin, 2.5%-25% of the eucalyptol, 1.25%-15% of the emulsifier, 0.3%-3.75% of the co-emulsifier and 55.3%-95.5% of the water, and sum thereof is 100%.
- the evenly mixing described in step (1) refers to stirring at 40-60° C. for 5-15 min at a speed of 200-500 rpm, and more preferably, stirring at 50° C. for 10 min at a speed of 300 rpm.
- the cyclodextrin described in step (1) includes one or more of ⁇ -cyclodextrin, ⁇ -cyclodextrin and ⁇ -cyclodextrin.
- the cyclodextrin solution described in step (1) has a mass concentration of 1%-2%.
- the cyclodextrin solution described in step (1) is prepared by adding the cyclodextrin to water, heating and stirring in a water bath at 40-60° C., and then mixing until the liquid is clear and transparent.
- the emulsifier described instep (2) includes one or more of polyoxyethylene castor oil, TWEEN® (polysorbate) and SPANTM (sorbitan oleate).
- the co-emulsifier described instep (2) is anhydrous ethanol.
- the evenly mixing described in step (2) refers to mixing at 50° C. to achieve a state of transparency and good fluidity.
- the emulsifying described in step (3) refers to stirring at 40-60° C. for 15-25 min at a speed of 200-500 rpm, and more preferably, stirring at 50° C. for 20 min at a speed of 300 rpm.
- the second object of the present disclosure is eucalyptol emulsion prepared by the method of the present disclosure.
- the third object of the present disclosure is application of the eucalyptol emulsion according to the present disclosure in the fields of medicine, daily chemicals and agriculture, especially in biopesticides.
- the cyclodextrin is introduced into the traditional emulsification technology and the eucalyptol is embedded by utilizing the “internal alienation and external affinity” property of the cyclodextrin to improve the stability of the eucalyptol, which provides a new method for enhancing the application performance of the eucalyptol.
- the emulsion prepared according to the present disclosure is extremely stable and does not stratify after being placed at 20° C., 40° C., and 60° C. for 30 days, which is beneficial to the storage, transportation and use of the product.
- the eucalyptol emulsion prepared according to the present disclosure has good dilution stability. Specifically, it can remain stable without stratification after being diluted in any proportion, thus obviously overcoming the defects of poor stability and insufficient water solubility of the eucalyptol.
- the eucalyptol emulsion product prepared according to the present disclosure has better insecticidal effect when being applied to the biopesticides.
- FIG. 1 is a view of a sample of eucalyptol emulsion in Example 1.
- FIG. 2 is a particle size measurement diagram of the eucalyptol emulsion in Example 1.
- the emulsion was placed in a centrifugal tube and stood still at different temperatures (20° C., 40° C., and 60° C.). The state of the emulsion was observed regularly with the naked eyes and recorded whether it was uniform and stable.
- the direct observation method was carried out: part of liquid was drawn from the prepared system for two-fold gradient dilution, then stored in the centrifuge tube and placed at room temperature. The state of diluent was regularly observed with the naked eyes.
- a laser particle size analyzer was used to test the particle size of the optimized system.
- the particle size distribution was represented by a particle size span.
- d 90 , d 50 and d 10 are the particle size values corresponding to 90%, 50% and 10% of the cumulative distribution of particle sizes, respectively.
- the virulence of the optimized system to Bemisiatabaci and aphids was determined by adopting an insect-dip method and a leaf-dip method. Specifically, 7 treatment concentrations, namely, 500 mg/L, 250 mg/L, 125 mg/L, 62.5 mg/L, 31.25 mg/L, 15.63 mg/L and 7.81 mg/L were set for an aphid treatment group, and another 7 treatment concentrations, namely, 250 mg/L, 125 mg/L, 62.5 mg/L, 31.25 mg/L, 15.63 mg/L, 7.81 mg/L and 3.91 mg/L were set for a B. tabaci treatment group. A clear water control was also set and each treatment was repeated for 4 times with 20-40 insects each time. The specific operations were as follows:
- Insect-dip method the selected aphids together with their leaves were dipped in liquid medicine for 5s and then taken out; the liquid medicine was sucked up with absorbent paper; and the aphids were put into a pre-moisturized disposable plastic culture cup lined with filter paper. 30-40 aphids were placed in each cup. Treatment was repeated for 4 times at each concentration and 120-160 aphids were treated at each concentration. The control was treated with water. The culture cup was put into an incubator at (26 ⁇ 2°) C. with a photoperiod of 16:8 (L:D). The results were checked after treatment for 48 h.
- Agar moisturizing leaf-dip method agar was prepared into 15-17 g/L with distilled water. 2 mL liquid agar was drawn with a micropipettor and added to the bottom of a flat-bottomed glass tube. Be careful not to contaminate the tube wall and produce air bubbles. Then, the liquid agar was cooled and solidified and steam on the tube wall was volatilized completely. The round leaves of with diameters of 18 mm in flowering cabbage leaves were separately dipped in liquid medicine of 7 series of concentrations for 5s. Next, leaves were taken out and dried at room temperature and the back sides thereof were adhered upward to the surface of the agar. The control was treated with distilled water. Adults of the B.
- a method for preparing eucalyptol emulsion included the following steps:
- the stability and dilution performance of the obtained eucalyptol emulsion were tested.
- the test results are as follows:
- the eucalyptol emulsion prepared according to Example 1 is stable and even does not stratify after being stored at 20° C., 40° C., and 60° C. for 30 days. Hence, the eucalyptol emulsion has good stability and is beneficial to the storage, transportation and use of the product. Furthermore, the eucalyptol emulsion can remain stable after being diluted in any proportion, which shows that the eucalyptol emulsion well solves the problems of poor stability and water solubility of eucalyptol.
- the eucalyptol emulsion was subjected to particle size analysis. The results are shown in FIG. 2 . It can be seen from FIG. 2 that the particle size span of the emulsion is 1.02, which is very close to 1, indicating that the particle size distribution in the emulsion is symmetrical. Besides, there is no phenomenon of too many large particles or small particles, so that it is easy for the emulsion to keep stable.
- Example 1 has good insecticidal effect and biopesticide application performance.
- LC 50 referring to a concentration of poison at which half of the pests under test die, can be used to evaluate the control effect of an insecticide on the pests well and objectively.
- the literature (Xie Ting, Jiang Ling, Hong Bo, et al. Virulence and Field Control Effect of Mixed BeauveriaBassiana and Matrineagainst B. tabaci [J]. ActaAgriculturaeBoreali-OccidentalisSinica, 2019, 28(05): 830-836) reported that the LC 50 of the matrine against the B. tabaci was 13.35 mg/Land that of the B.
- bassiana was 7.86 mg/L. It can be seen that the control effect of the emulsion in Example 1 on the B. tabaciis better than or basically the same as that of existing biopesticide products.
- the literature Wang Zibang, Wei Shuqin. Indoor Virulence Determination and Field Control Effect of 3 Kinds of Botanical Pesticides on Blueberry Aphids [J]. South China Fruits, 2020, 49(06):133-135+140) reported that the LC 50 of a 1.3% matrine aqueous solution against aphids was 19.01 mg/L and that of 0.3% azadirachtin emulsion was 11.20 mg/L and that of 6.0% rotenone micro emulsion was 34.75 mg/L. It can also be seen that the control effect of the emulsion in Example 1 on the aphids is better than or basically the same as that of existing biopesticide products.
- the eucalyptol amount in step (2) according to Example 1 was adjusted to 2 g, 5 g, 10 g, 30 g, 40 g, 50 g and 60 g; the water content in step (3) was adjusted accordingly to maintain the total system mass at 200 g; and others were maintained to be consistent with those in Example 1.
- Example 2 Eucalyptol application Stability amount (g) 20° C. 40° C. 60° C. Dilution performance 20 (Example 1) Uniform Uniform and Uniform and Diluted in any proportion and stable stable stable 2 Stratified and Stratified and Stratified and All stratified unstable unstable unstable 5 Uniform Uniform and Uniform and Diluted in any proportion and stable stable stable 10 Uniform Uniform and Uniform and Diluted in any proportion and stable stable stable 30 Uniform Uniform and Uniform and Diluted in any proportion and stable stable stable 40 Uniform Uniform and Uniform and Diluted in any proportion and stable stable stable 50 Uniform Uniform and Uniform and Diluted in any proportion and stable stable stable 60 Uniform Stratified and Stratified and 1-10 times dilutable, other and stable unstable unstable times stratified
- the newly prepared emulsion is uniform and stable and can maintain a stable state after being placed at 20° C. for 30 days.
- the emulsion is all stratified after being placed at 40° C. and 60° C. for 30 days, indicating that it is poor in thermal stability.
- the emulsion which contains more than 30% eucalyptol is not conducive to the storage and transportation.
- the emulsion can only be diluted by a factor of 1 to 10 when in use and further dilution will destroy its water-soluble properties and cause stratification.
- the cyclodextrin amount in step (1) according to Example 1 was adjusted to 0 g, 0.5 g, 1 g, 2 g and 2.5 g; the water amount in step was adjusted to maintain the mass of a cyclodextrin solution at 100 g; and others were maintained to be consistent with those in Example 1.
- Example 3 Cyclodextrin application Stability Dilution amount (g) 20° C. 40° C. 60° C. performance 1.5 (Example 1) Uniform and stable Uniform and stable Uniform and stable Diluted in any proportion 0 Stratified and Stratified and Stratified and All stratified unstable unstable unstable 0.5 Stratified and Stratified and Stratified and All stratified unstable unstable unstable 1 Uniform and stable Uniform and stable Uniform and stable Diluted in any proportion 2 Uniform and stable Uniform and stable Uniform and stable Diluted in any proportion 2.5 Precipitated and Precipitated and Precipitated and Precipitated and All precipitated unstable unstable unstable unstable
- the emulsifier amount in step (3) according to Example 1 was adjusted to 0 g, 0.5 g, 1 g, 2.5 g, 5 g, 20 g, 30 g and 40 g; the water content in step (3) was adjusted accordingly to maintain the total system mass at 200 g; and others were maintained to be consistent with those in Example 1.
- Example 4 Test results of Example 4 Emulsifier application Stability Dilution amount (g) 20° C. 40° C. 60° C. performance 10 (Example 1) Uniform and stable Uniform and stable Uniform and stable Diluted in any proportion 0 Stratified and Stratified and Stratified and All stratified unstable unstable unstable 0.5 Stratified and Stratified and Stratified and All stratified unstable unstable unstable 1 Stratified and Stratified and Stratified and All stratified unstable unstable unstable 2.5 Uniform and stable Uniform and stable Uniform and stable Diluted in any proportion 5 Uniform and stable Uniform and stable Uniform and stable Diluted in any proportion 20 Uniform and stable Uniform and stable Uniform and stable Diluted in any proportion 30 Uniform and stable Uniform and stable Uniform and stable Diluted in any proportion 40 Uniform, stable but Uniform, stable but Uniform, stable but Diluted in any viscous viscous proportion
- the eucalyptol emulsion When the emulsifier content is within a range of 1.25%-15%, the eucalyptol emulsion is relatively stable. The eucalyptol emulsion can remain stable without stratification after being stored at 20° C., 40° C., and 60° C. for 30 days or after being diluted with water in any proportion. However, when the emulsifier addition reaches 20%, the product has high viscosity and poor fluidity due to the high emulsifier content, which is not conducive to daily use and increases the preparation cost.
- the emulsifier types in step (3) according to Example 1 were adjusted as TWEEN® 80 (polysorbate 80, CAS No. 9005-65-6), a compound of TWEEN® 80 and SPAN′ 60 (sorbitan monostearate, CAS No. 1338-41-6, in a mass ratio of 8:2), polyoxyethyleneoctyl phenol ether-10, sodium lauroyl sarcosine (LS-30N) or polyethylene glycol 40 (PEG-40) hydrogenated castor oil, which were commonly used to produce oil-in-water emulsion. Besides, other components were consistent with those in Example 1.
- Example 5 Stability Emulsifier 20° C. 40° C. 60° C. Dilution performance Polyoxyethylene castor Uniform and Uniform Uniform and Diluted in any proportion oil 30 stable and stable stable TWEEN ® 80 Uniform and Uniform Uniform and 1-10 times dilutable, other stable and stable stable times stratified Compound of Uniform and Uniform Uniform and Diluted in any proportion TWEEN ® 80 stable and stable stable and SPAN TM 60 (8:2) Polyoxyethyleneoctyl Stratified Stratified and Stratified and All stratified phenol ether-10 and unstable unstable unstable Sodium lauroyl sarcosine Stratified Stratified and Stratified and All stratified (LS-30N) and unstable unstable unstable PEG-40 hydrogenated Stratified Stratified and Stratified and All stratified castor oil and unstable unstable unstable unstable
- the eucalyptol emulsion is relatively stable.
- the eucalyptol emulsion can remain stable without stratification after being placed at 20° C., 40° C., and 60° C. for 30 days.
- the eucalyptol emulsion prepared with a mixture of the TWEEN® 80 and SPANTM 60 has better dilution performance than TWEEN® 80's, because the emulsifying property of TWEEN® 80 and SPANTM 60 can be improved by the combination.
- TWEEN® and SPANTM both have the structure of dehydrated sorbitol fatty acid ester. Furthermore, when high and low HLB emulsifiers are mixed, they can be adsorbed on an interface to form tightly packed “complexes”, which increases the stability of the emulsion.
- the HLB value of the TWEEN® 80 is about 15 and the HLB value of the SPANTM 60 is about 4.7.
- both are relatively suitable for compound emulsifier.
- the SPANTM 60 is solid powder which is difficult to uniformly disperse in the system, the complexity and time of operation will be increased when it is used.
- SPANTM 60 is not considered as the optimal solution.
- the emulsion with the TWEEN® 80 as the emulsifier can only be diluted at 1-10 times. Further dilution will destroy the water solubility of the emulsion and cause stratification, so that TWEEN® 80 is not considered an ideal emulsifier.
- the polyoxyethyleneoctyl phenol ether-10, the sodium lauroyl sarcosine (LS-30N) and the PEG-40 hydrogenated castor oil are used as emulsifiers, the eucalyptol emulsion is unstable and stratified rapidly, which may be due to the incompatibility of these emulsifiers with the system or the preparation method.
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Abstract
Description
Span=(d 90 −d 10)/d 50 (1)
TABLE 1 |
Evaluation results of insecticidal performance |
Confidence | Correlation | |||
Regression equation | LC50 (mg/L) | interval | coefficient R2 | |
B. tabaci | Y = 1.388X − 1.283 | 8.40 | 6.24-10.73 | 0.983 |
Aphids (insect- | Y = 1.279X − 1.439 | 13.34 | 9.61-17.20 | 0.973 |
dip method) | ||||
TABLE 2 |
Test results of Example 2 |
Eucalyptol | ||
application | Stability |
amount (g) | 20° C. | 40° C. | 60° C. | Dilution performance |
20 (Example 1) | Uniform | Uniform and | Uniform and | Diluted in any proportion |
and stable | stable | stable | ||
2 | Stratified and | Stratified and | Stratified and | All stratified |
unstable | unstable | unstable | ||
5 | Uniform | Uniform and | Uniform and | Diluted in any proportion |
and stable | stable | stable | ||
10 | Uniform | Uniform and | Uniform and | Diluted in any proportion |
and stable | stable | stable | ||
30 | Uniform | Uniform and | Uniform and | Diluted in any proportion |
and stable | stable | stable | ||
40 | Uniform | Uniform and | Uniform and | Diluted in any proportion |
and stable | stable | stable | ||
50 | Uniform | Uniform and | Uniform and | Diluted in any proportion |
and stable | stable | stable | ||
60 | Uniform | Stratified and | Stratified and | 1-10 times dilutable, other |
and stable | unstable | unstable | times stratified | |
TABLE 3 |
Test results of Example 3 |
Cyclodextrin | ||
application | Stability | Dilution |
amount (g) | 20° C. | 40° C. | 60° C. | performance |
1.5 (Example 1) | Uniform and stable | Uniform and stable | Uniform and stable | Diluted in any |
|
||||
0 | Stratified and | Stratified and | Stratified and | All stratified |
unstable | unstable | unstable | ||
0.5 | Stratified and | Stratified and | Stratified and | All stratified |
unstable | unstable | unstable | ||
1 | Uniform and stable | Uniform and stable | Uniform and stable | Diluted in any |
proportion | ||||
2 | Uniform and stable | Uniform and stable | Uniform and stable | Diluted in any |
proportion | ||||
2.5 | Precipitated and | Precipitated and | Precipitated and | All precipitated |
unstable | unstable | unstable | ||
TABLE 4 |
Test results of Example 4 |
Emulsifier | ||
application | Stability | Dilution |
amount (g) | 20° C. | 40° C. | 60° C. | performance |
10 (Example 1) | Uniform and stable | Uniform and stable | Uniform and stable | Diluted in any |
|
||||
0 | Stratified and | Stratified and | Stratified and | All stratified |
unstable | unstable | unstable | ||
0.5 | Stratified and | Stratified and | Stratified and | All stratified |
unstable | unstable | unstable | ||
1 | Stratified and | Stratified and | Stratified and | All stratified |
unstable | unstable | unstable | ||
2.5 | Uniform and stable | Uniform and stable | Uniform and stable | Diluted in any |
proportion | ||||
5 | Uniform and stable | Uniform and stable | Uniform and stable | Diluted in any |
|
||||
20 | Uniform and stable | Uniform and stable | Uniform and stable | Diluted in any |
|
||||
30 | Uniform and stable | Uniform and stable | Uniform and stable | Diluted in any |
|
||||
40 | Uniform, stable but | Uniform, stable but | Uniform, stable but | Diluted in any |
viscous | viscous | viscous | proportion | |
TABLE 5 |
Test results of Example 5 |
|
Emulsifier |
20° C. | 40° C. | 60° C. | Dilution performance | |
Polyoxyethylene castor | Uniform and | Uniform | Uniform and | Diluted in any |
oil | ||||
30 | stable | and stable | | |
TWEEN ® | ||||
80 | Uniform and | Uniform | Uniform and | 1-10 times dilutable, other |
stable | and stable | stable | times stratified | |
Compound of | Uniform and | Uniform | Uniform and | Diluted in any |
TWEEN ® | ||||
80 | stable | and stable | stable | |
and SPAN ™ 60 (8:2) | ||||
Polyoxyethyleneoctyl | Stratified | Stratified and | Stratified and | All stratified |
phenol ether-10 | and unstable | unstable | unstable | |
Sodium lauroyl sarcosine | Stratified | Stratified and | Stratified and | All stratified |
(LS-30N) | and unstable | unstable | unstable | |
PEG-40 hydrogenated | Stratified | Stratified and | Stratified and | All stratified |
castor oil | and unstable | unstable | unstable | |
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