UA6152A1 - СПОСІБ ОДЕРЖАННЯ ШТАММІВ БАКТЕРІЙ - ESCHERICHIA COLI i PSEUDOMONAS PURIDA - ПРОДУЦЕНТІВ КРЕАТІНАМІДІНОГІДРОЛАЗИ - Google Patents
СПОСІБ ОДЕРЖАННЯ ШТАММІВ БАКТЕРІЙ - ESCHERICHIA COLI i PSEUDOMONAS PURIDA - ПРОДУЦЕНТІВ КРЕАТІНАМІДІНОГІДРОЛАЗИInfo
- Publication number
- UA6152A1 UA6152A1 UA4002500A UA4002500A UA6152A1 UA 6152 A1 UA6152 A1 UA 6152A1 UA 4002500 A UA4002500 A UA 4002500A UA 4002500 A UA4002500 A UA 4002500A UA 6152 A1 UA6152 A1 UA 6152A1
- Authority
- UA
- Ukraine
- Prior art keywords
- phages
- dna
- producers
- escherichia coli
- treated
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/848—Escherichia
- Y10S435/849—Escherichia coli
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/874—Pseudomonas
- Y10S435/877—Pseudomonas putida
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Спосіб одержання штамів бактерій Еsсhеrісhіа соli i Pseudomonas putida - продуцентів креатинамідиногідролази полягає в тому, що хромосомну ДНК із Pseudomonas putida DSM 2106 із ДНК фага Шарон 10 обробляють ендонуклеазою Eco.RI. Лігують одержані фрагменти, упаковують гібридні ДНК в пристуності 2-х протеїнів оболонки фага. Трансдукують одержаними гібридними фагами клітини Е.соlі DSМ 2102, котрі попередньо обробляють протягом ночі 0,2 % -вим розчином мальтози та розчиняють в 0,1 М/л МgSO4. потім ідентифікують фаги, що експресують креатинамідиногідролазу за допомогою індикаторної системи, що містить 6 мг/мл тетраметилбензидину, 20мг/мл діоктилнатрійсукцинату і 0,01 % Н202 в 6 %-вій желатині. Виділяють фаги, що дають позитивну реакцію. ДНК цих фагів обробляють ендонуклеазою Есо.RІ з подальшим виділенням фрагмента розміром 5,8 кВ.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19853500184 DE3500184A1 (de) | 1985-01-04 | 1985-01-04 | Mikroorganismus und plasmid fuer konstitutive creatinamidinohydrolase-bildung sowie verfahren zur herstellung derselben |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA6152A1 true UA6152A1 (uk) | 1994-12-29 |
Family
ID=6259284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UA4002500A UA6152A1 (uk) | 1985-01-04 | 1986-01-03 | СПОСІБ ОДЕРЖАННЯ ШТАММІВ БАКТЕРІЙ - ESCHERICHIA COLI i PSEUDOMONAS PURIDA - ПРОДУЦЕНТІВ КРЕАТІНАМІДІНОГІДРОЛАЗИ |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US4861717A (uk) |
| EP (1) | EP0187138B1 (uk) |
| JP (2) | JPH062050B2 (uk) |
| AT (1) | ATE74964T1 (uk) |
| AU (1) | AU561319B2 (uk) |
| CA (1) | CA1309960C (uk) |
| CZ (1) | CZ279427B6 (uk) |
| DE (2) | DE3500184A1 (uk) |
| DK (1) | DK3086A (uk) |
| ES (1) | ES8704543A1 (uk) |
| GR (1) | GR853154B (uk) |
| IE (1) | IE58794B1 (uk) |
| SK (1) | SK278079B6 (uk) |
| SU (1) | SU1523055A3 (uk) |
| UA (1) | UA6152A1 (uk) |
| YU (1) | YU204985A (uk) |
| ZA (1) | ZA8630B (uk) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3500184A1 (de) * | 1985-01-04 | 1986-08-14 | Boehringer Mannheim Gmbh, 6800 Mannheim | Mikroorganismus und plasmid fuer konstitutive creatinamidinohydrolase-bildung sowie verfahren zur herstellung derselben |
| JPS62205786A (ja) * | 1986-03-07 | 1987-09-10 | Kikkoman Corp | 新規な組み換え体ｄｎａ |
| JPS62208281A (ja) * | 1986-03-07 | 1987-09-12 | Kikkoman Corp | クレアチナ−ゼの製造法 |
| DE3803175A1 (de) * | 1987-05-12 | 1988-11-24 | Boehringer Mannheim Gmbh | Stabile creatinamidinohydrolase-mutanten |
| JPS6437292A (en) * | 1987-08-04 | 1989-02-07 | Toyo Jozo Kk | Dna having genetic information of creatinase and use thereof |
| JP2527035B2 (ja) * | 1989-06-16 | 1996-08-21 | 東洋紡績株式会社 | クレアチニン・アミドヒドロラ―ゼをコ―ドする遺伝子を含有するｄｎａ断片、該ｄｎａ断片を有する組換えベクタ―及び該組換えベクタ―を有する形質転換体、並びにクレアチニン・アミドヒドロラ―ゼの製造方法 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4039384A (en) * | 1975-04-05 | 1977-08-02 | Noda Institute For Scientific Research | Creatinine amidohydrolase and creatine amidinohydrolase and process for producing them |
| JPS51118884A (en) * | 1975-04-05 | 1976-10-19 | Noda Sangyo Kagaku Kenkyusho | Process for preparing creatine amidinohydrolase |
| DE3500184A1 (de) * | 1985-01-04 | 1986-08-14 | Boehringer Mannheim Gmbh, 6800 Mannheim | Mikroorganismus und plasmid fuer konstitutive creatinamidinohydrolase-bildung sowie verfahren zur herstellung derselben |
-
1985
- 1985-01-04 DE DE19853500184 patent/DE3500184A1/de not_active Withdrawn
- 1985-12-24 AU AU51631/85A patent/AU561319B2/en not_active Ceased
- 1985-12-26 JP JP60292383A patent/JPH062050B2/ja not_active Expired - Lifetime
- 1985-12-27 YU YU204985A patent/YU204985A/sh unknown
- 1985-12-30 GR GR853154A patent/GR853154B/el unknown
-
1986
- 1986-01-02 CA CA000498900A patent/CA1309960C/en not_active Expired - Lifetime
- 1986-01-03 IE IE1686A patent/IE58794B1/en not_active IP Right Cessation
- 1986-01-03 CZ CS8675A patent/CZ279427B6/cs unknown
- 1986-01-03 AT AT86100066T patent/ATE74964T1/de not_active IP Right Cessation
- 1986-01-03 ES ES550669A patent/ES8704543A1/es not_active Expired
- 1986-01-03 SK SK75-86A patent/SK278079B6/sk unknown
- 1986-01-03 DE DE8686100066T patent/DE3684787D1/de not_active Expired - Lifetime
- 1986-01-03 EP EP86100066A patent/EP0187138B1/de not_active Expired - Lifetime
- 1986-01-03 UA UA4002500A patent/UA6152A1/uk unknown
- 1986-01-03 DK DK3086A patent/DK3086A/da not_active Application Discontinuation
- 1986-01-03 SU SU864002500A patent/SU1523055A3/ru active
- 1986-01-03 ZA ZA8630A patent/ZA8630B/xx unknown
- 1986-01-06 US US06/816,565 patent/US4861717A/en not_active Expired - Fee Related
-
1993
- 1993-07-30 JP JP5189871A patent/JPH07102140B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE3500184A1 (de) | 1986-08-14 |
| YU204985A (sh) | 1992-09-07 |
| EP0187138A3 (en) | 1988-03-23 |
| CZ7586A3 (en) | 1994-11-16 |
| EP0187138A2 (de) | 1986-07-09 |
| IE860016L (en) | 1986-07-04 |
| CA1309960C (en) | 1992-11-10 |
| EP0187138B1 (de) | 1992-04-15 |
| JPH062050B2 (ja) | 1994-01-12 |
| JPH06233687A (ja) | 1994-08-23 |
| GR853154B (uk) | 1986-04-24 |
| JPS61162170A (ja) | 1986-07-22 |
| SK7586A3 (en) | 1995-12-06 |
| AU5163185A (en) | 1986-07-10 |
| ATE74964T1 (de) | 1992-05-15 |
| CZ279427B6 (cs) | 1995-04-12 |
| SK278079B6 (en) | 1995-12-06 |
| DE3684787D1 (de) | 1992-05-21 |
| DK3086A (da) | 1986-07-05 |
| JPH07102140B2 (ja) | 1995-11-08 |
| ZA8630B (en) | 1986-09-24 |
| ES550669A0 (es) | 1987-04-16 |
| ES8704543A1 (es) | 1987-04-16 |
| DK3086D0 (da) | 1986-01-03 |
| IE58794B1 (en) | 1993-11-17 |
| US4861717A (en) | 1989-08-29 |
| SU1523055A3 (ru) | 1989-11-15 |
| AU561319B2 (en) | 1987-05-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPS6486896A (en) | Method for isolating basic protein from protein mixture containing the basic protein | |
| AU524856B2 (en) | Method of making a selected protein | |
| ES2046177T3 (es) | Construccion de una proteina enlazante a igg para facilitar el tratamiento aguas abajo utilizando ingenieria de proteinas. | |
| AU531566B2 (en) | Protein biosynthesis using a transformed bacterium | |
| DK609985D0 (da) | Adhesin-antigener, antistoffer mod disse og dna-fragmenter, som indkoder antigenerne | |
| Cao et al. | Translocation of N‐terminal tails across the plasma membrane. | |
| NO176807C (no) | Sammensmeltet gen som koder for et hybridprotein, samt fremgangsmåte for fremstilling av et hybridprotein som kodes av et sammensmeltet gen; vektor og sammensmeltet gen | |
| UA6152A1 (uk) | СПОСІБ ОДЕРЖАННЯ ШТАММІВ БАКТЕРІЙ - ESCHERICHIA COLI i PSEUDOMONAS PURIDA - ПРОДУЦЕНТІВ КРЕАТІНАМІДІНОГІДРОЛАЗИ | |
| Ross et al. | The Bacillus subtilis HBsu protein modifies the effects of α/β-type, small acid-soluble spore proteins on DNA | |
| HK86595A (en) | Avirulent vibrio cholerae and methods for its production | |
| Randall et al. | Processing in vitro of precursor periplasmic proteins from Escherichia coli | |
| Imajoh et al. | The receptor for colicin E3. Isolation and some properties. | |
| BR8306108A (pt) | Novas enzimas fibrinoliticas processos para sua producao e composicoes farmaceuticas que as contem | |
| Chu et al. | Role for recombinant γ-glutamyltransferase from Treponema denticola in glutathione metabolism | |
| Erdös et al. | Floating equilibrium of symmetrical objects and the breaking of symmetry. Part 2: The cube, the octahedron, and the tetrahedron | |
| UA27771C2 (uk) | Електролітична комірка (варіанти) та спосіб мічення протеїнів, пептидів і інших органічних молекул | |
| Jakobsen et al. | Decapod Crustacea from the Palaeocene of Zealand, Denmark | |
| JPS5523994A (en) | Novel microorganism | |
| FI905055A0 (fi) | Ett p1-bakteriofagkloningssystem foer sao stora dna-fragment som 95 kb. | |
| ES8603956A1 (es) | Un metodo de ensayar la expresion de genes | |
| Hayashi et al. | Cell-free synthesis of ornithine aminotransferase of rat liver | |
| AU564040B2 (en) | 1,2,3,4 - tetrahydro-1-aminomethyl-4-phenyl - isoquinolines | |
| SUGAI | Studies on streptolysin S'formation by streptococcal ghosts | |
| Whealton et al. | High brightness ion source: The Penning ringatron | |
| Nikitin et al. | Calculation of recombination line intensities for C III ions. |