UA118328C2 - Спосіб інтегрування екзогенних послідовностей у геном рослинної клітини - Google Patents

Спосіб інтегрування екзогенних послідовностей у геном рослинної клітини

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Publication number
UA118328C2
UA118328C2 UAA201210055A UAA201210055A UA118328C2 UA 118328 C2 UA118328 C2 UA 118328C2 UA A201210055 A UAA201210055 A UA A201210055A UA A201210055 A UAA201210055 A UA A201210055A UA 118328 C2 UA118328 C2 UA 118328C2
Authority
UA
Ukraine
Prior art keywords
targeted
gemomic
alteration
polypeptides
sequences
Prior art date
Application number
UAA201210055A
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English (en)
Inventor
Уілльям М. Ейнлі
Майкл Г. Мюррей
Фьодор Урнов
Брайан Цайтлер
Original Assignee
Дау Агросайєнсіз Ллс
Сангамо Терап’Ютікс, Інк.
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Application filed by Дау Агросайєнсіз Ллс, Сангамо Терап’Ютікс, Інк. filed Critical Дау Агросайєнсіз Ллс
Publication of UA118328C2 publication Critical patent/UA118328C2/uk

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Винахід стосується ізольованої рослинної клітини, яка містить ендогенний геном і екзогенну нуклеїнову кислоту, інтегровану в ендогенний геном. Екзогенна нуклеїнова кислота містить сайт множинної інсерції, причому зазначений сайт множинної інсерції містить три або більше різних парних сайтів-мішеней для однієї або декількох пар нуклеаз цинкового пальця. Винахід також стосується способу інтегрування однієї або більше екзогенних послідовностей у геном рослинної клітини.
UAA201210055A 2010-01-22 2011-01-24 Спосіб інтегрування екзогенних послідовностей у геном рослинної клітини UA118328C2 (uk)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33645710P 2010-01-22 2010-01-22
PCT/US2011/000125 WO2011090804A1 (en) 2010-01-22 2011-01-24 Targeted genomic alteration

Publications (1)

Publication Number Publication Date
UA118328C2 true UA118328C2 (uk) 2019-01-10

Family

ID=44307145

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201210055A UA118328C2 (uk) 2010-01-22 2011-01-24 Спосіб інтегрування екзогенних послідовностей у геном рослинної клітини

Country Status (18)

Country Link
US (1) US10260062B2 (uk)
EP (1) EP2526112B1 (uk)
JP (1) JP5902631B2 (uk)
KR (1) KR101866578B1 (uk)
CN (2) CN106086047B (uk)
AR (1) AR080009A1 (uk)
AU (1) AU2011207769B2 (uk)
BR (1) BR112012018249A2 (uk)
CA (1) CA2787494C (uk)
CL (1) CL2012002033A1 (uk)
CO (1) CO6561839A2 (uk)
EA (1) EA031322B1 (uk)
GE (1) GEP20176628B (uk)
IL (1) IL221029B (uk)
MX (1) MX336846B (uk)
NZ (1) NZ601247A (uk)
UA (1) UA118328C2 (uk)
WO (1) WO2011090804A1 (uk)

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WO2011090804A1 (en) 2011-07-28
US10260062B2 (en) 2019-04-16
CN102812034B (zh) 2016-08-03
JP2013517770A (ja) 2013-05-20
AU2011207769A1 (en) 2012-08-02
CA2787494A1 (en) 2011-07-28
EA031322B1 (ru) 2018-12-28
BR112012018249A2 (pt) 2020-02-04
CO6561839A2 (es) 2012-11-15
AR080009A1 (es) 2012-03-07
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GEP20176628B (en) 2017-02-27
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MX336846B (es) 2016-02-03
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EP2526112A4 (en) 2013-12-04
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MX2012008490A (es) 2013-04-05
US20110189775A1 (en) 2011-08-04
CL2012002033A1 (es) 2012-12-14
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