TWI835779B - 改善生產生物反應器中細胞存活力之方法 - Google Patents

改善生產生物反應器中細胞存活力之方法 Download PDF

Info

Publication number
TWI835779B
TWI835779B TW108108883A TW108108883A TWI835779B TW I835779 B TWI835779 B TW I835779B TW 108108883 A TW108108883 A TW 108108883A TW 108108883 A TW108108883 A TW 108108883A TW I835779 B TWI835779 B TW I835779B
Authority
TW
Taiwan
Prior art keywords
day
per day
cell
cells
ppm
Prior art date
Application number
TW108108883A
Other languages
English (en)
Chinese (zh)
Other versions
TW201947033A (zh
Inventor
強納森 王
涅哈 尚
詹森 瓦爾瑟
久怡 陸
堤摩西 強森
仼玉坤
吉恩 馬克拉堤
Original Assignee
美商健臻公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 美商健臻公司 filed Critical 美商健臻公司
Publication of TW201947033A publication Critical patent/TW201947033A/zh
Application granted granted Critical
Publication of TWI835779B publication Critical patent/TWI835779B/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
TW108108883A 2018-03-16 2019-03-15 改善生產生物反應器中細胞存活力之方法 TWI835779B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862644339P 2018-03-16 2018-03-16
US62/644,339 2018-03-16
US201862645755P 2018-03-20 2018-03-20
US62/645,755 2018-03-20

Publications (2)

Publication Number Publication Date
TW201947033A TW201947033A (zh) 2019-12-16
TWI835779B true TWI835779B (zh) 2024-03-21

Family

ID=65952204

Family Applications (2)

Application Number Title Priority Date Filing Date
TW108108883A TWI835779B (zh) 2018-03-16 2019-03-15 改善生產生物反應器中細胞存活力之方法
TW113106091A TW202513790A (zh) 2018-03-16 2019-03-15 改善生產生物反應器中細胞存活力之方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
TW113106091A TW202513790A (zh) 2018-03-16 2019-03-15 改善生產生物反應器中細胞存活力之方法

Country Status (18)

Country Link
US (2) US11391725B2 (https=)
EP (2) EP3765846B1 (https=)
JP (2) JP7816863B2 (https=)
KR (2) KR102757905B1 (https=)
CN (2) CN119709931A (https=)
AU (1) AU2019236246B2 (https=)
BR (1) BR112020018823A2 (https=)
CA (1) CA3094076C (https=)
ES (1) ES3009097T3 (https=)
HU (1) HUE069711T2 (https=)
IL (2) IL319295A (https=)
MA (1) MA52007A (https=)
MX (2) MX2020009605A (https=)
PL (1) PL3765846T3 (https=)
SG (1) SG11202009047TA (https=)
TW (2) TWI835779B (https=)
WO (1) WO2019178508A1 (https=)
ZA (2) ZA202005741B (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4410957A4 (en) * 2021-09-30 2026-01-28 Fujifilm Corp CELL CULTURE PROCESS AND PROCESS FOR PRODUCING USEFUL SUBSTANCE
EP4594462A1 (en) 2022-09-28 2025-08-06 Genzyme Corporation Systems and devices for producing biological products and methods of using the same
US20240263122A1 (en) * 2023-02-08 2024-08-08 National Central University Bioreactor apparatus, ion-exchange substrate and method for regulation of ion concentration and cell culture thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080145364A1 (en) * 2005-02-15 2008-06-19 Yale University Method for High Throughput Screening for Anitbodies and Proteins Inducing Apoptosis

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5563068A (en) * 1994-04-21 1996-10-08 Genetic Therapy, Inc. Bioreactor
AU2007294731B2 (en) * 2006-09-13 2014-04-17 Abbvie Inc. Cell culture improvements
EP2500416A1 (en) * 2006-09-13 2012-09-19 Abbott Laboratories Cell culture improvements
AU2008246928B2 (en) * 2007-05-07 2014-04-17 Protalix Ltd. Large scale disposable bioreactor
WO2010120514A2 (en) 2009-03-31 2010-10-21 The Trustees Of The University Of Pennsylvania Antigen-binding proteins comprising recombinant protein scaffolds
CN104962539B (zh) * 2009-07-31 2019-09-17 百深公司 用于adamts蛋白表达的细胞培养基
EP2943563A1 (en) * 2013-01-10 2015-11-18 Biogen MA Inc. Medium supplements for improved process performance
SG10201806083RA (en) * 2013-02-22 2018-08-30 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof
WO2014151901A1 (en) * 2013-03-14 2014-09-25 Abbvie Inc. Improvement of mammalian cell culture performance through surfactant supplementation of feed media
CA2905180C (en) * 2013-03-15 2023-03-07 Alder Biopharmaceuticals, Inc. Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
EP3003325A4 (en) * 2013-05-29 2017-01-11 Biogen MA Inc. Methods of evaluating cell culture additives
BR112016017660B1 (pt) * 2014-02-27 2022-04-19 F. Hoffmann-La Roche Ag Método para a produção de uma glicoproteína recombinante
SG10202001034PA (en) * 2014-12-22 2020-04-29 Genzyme Corp Methods of culturing a mammalian cell
TWI541353B (zh) * 2014-12-25 2016-07-11 財團法人工業技術研究院 產生嗜熱性鹼性蛋白酶生產菌屬（Ｔｅｐｉｄｉｍｏｎａｓ）之胞外蛋白的方法
CN108350440A (zh) * 2015-08-17 2018-07-31 阿雷克森制药公司 碱性磷酸酯的制造

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080145364A1 (en) * 2005-02-15 2008-06-19 Yale University Method for High Throughput Screening for Anitbodies and Proteins Inducing Apoptosis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
期刊 Magnus Schroeder, et al., "Differentiation and lineage selection of mouse embryonic stem cells in a stirred bench scale bioreactor with automated process control", Biotechnology and Bioenginnering, Vol.92, No.7, 無, 27 September 2005, Pages 920-933; *
期刊 Ningning Ma, et al., "Quantitative Studies of Cell-Bubble Interactions and Cell Damage at Different Pluronic F-68 and Cell Concentrations", Biotechnology Progress, Vol.20, Issue 4, 無, 05 September 2008, Pages 1183-1194 *

Also Published As

Publication number Publication date
CN112136045A (zh) 2020-12-25
IL277352A (en) 2020-11-30
AU2019236246A1 (en) 2020-11-12
EP3765846B1 (en) 2024-10-30
BR112020018823A2 (pt) 2021-02-09
WO2019178508A1 (en) 2019-09-19
CN119709931A (zh) 2025-03-28
JP7816863B2 (ja) 2026-02-18
IL277352B1 (en) 2025-04-01
CA3094076A1 (en) 2019-09-19
EP3765846A1 (en) 2021-01-20
EP3765846C0 (en) 2024-10-30
KR102757905B1 (ko) 2025-01-22
MA52007A (fr) 2021-01-20
IL277352B2 (en) 2025-08-01
ZA202005741B (en) 2023-04-26
US11391725B2 (en) 2022-07-19
KR20250016476A (ko) 2025-02-03
PL3765846T3 (pl) 2025-03-31
EP4512887A2 (en) 2025-02-26
IL319295A (en) 2025-04-01
CN112136045B (zh) 2024-12-13
JP2024023489A (ja) 2024-02-21
JP2021515584A (ja) 2021-06-24
TW202513790A (zh) 2025-04-01
AU2019236246B2 (en) 2025-06-05
US20220308042A1 (en) 2022-09-29
MX2020009605A (es) 2021-01-08
ES3009097T3 (en) 2025-03-26
EP4512887A3 (en) 2025-04-30
SG11202009047TA (en) 2020-10-29
HUE069711T2 (hu) 2025-04-28
MX2024009056A (es) 2024-07-29
US20190285617A1 (en) 2019-09-19
TW201947033A (zh) 2019-12-16
CA3094076C (en) 2025-11-25
RU2020133815A (ru) 2022-04-19
ZA202208613B (en) 2025-01-29
KR20200132932A (ko) 2020-11-25

Similar Documents

Publication Publication Date Title
JP7769663B2 (ja) 灌流培養方法およびその使用
US20220308042A1 (en) Methods for Improving Cell Viability In A Production BioReactor
US20200255798A1 (en) Methods of perfusion culturing using a shake flask and microcarriers
CN104903436A (zh) 灌注培养方法及其用途
US20160177361A1 (en) Methods of culturing a mammalian cell
EP3207121B1 (en) Methods of replicating a large scale eculizumab production cell culture
CA2901940A1 (en) Microcarrier perfusion culturing methods and uses thereof
RU2786815C2 (ru) Способы прогнозирования жизнеспособности клеток
HK40120937A (en) Methods for improving cell viability in a production bioreactor
HK40036189A (en) Methods for improving cell viability in a production bioreactor
HK40036189B (en) Methods for improving cell viability in a production bioreactor
CN107974432A (zh) 高效分泌表达猪瘟e2蛋白的cho细胞株的培养方法及其应用
HK40005716A (en) Microcarrier perfusion culturing methods and uses thereof