CN119709931A - 提高生产生物反应器中的细胞活力的方法 - Google Patents

提高生产生物反应器中的细胞活力的方法 Download PDF

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Publication number
CN119709931A
CN119709931A CN202411691320.6A CN202411691320A CN119709931A CN 119709931 A CN119709931 A CN 119709931A CN 202411691320 A CN202411691320 A CN 202411691320A CN 119709931 A CN119709931 A CN 119709931A
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China
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day
cell
per day
daily
cells
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Pending
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CN202411691320.6A
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English (en)
Chinese (zh)
Inventor
J·王
N·沙
J·沃尔特
陆久怡
T·约翰逊
任玉坤
J·麦卡蒂
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Genzyme Corp
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Genzyme Corp
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Publication of CN119709931A publication Critical patent/CN119709931A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN202411691320.6A 2018-03-16 2019-03-15 提高生产生物反应器中的细胞活力的方法 Pending CN119709931A (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201862644339P 2018-03-16 2018-03-16
US62/644,339 2018-03-16
US201862645755P 2018-03-20 2018-03-20
US62/645,755 2018-03-20
CN201980031298.3A CN112136045B (zh) 2018-03-16 2019-03-15 提高生产生物反应器中的细胞活力的方法
PCT/US2019/022530 WO2019178508A1 (en) 2018-03-16 2019-03-15 Methods for improving cell viability in a production bioreactor

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201980031298.3A Division CN112136045B (zh) 2018-03-16 2019-03-15 提高生产生物反应器中的细胞活力的方法

Publications (1)

Publication Number Publication Date
CN119709931A true CN119709931A (zh) 2025-03-28

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CN202411691320.6A Pending CN119709931A (zh) 2018-03-16 2019-03-15 提高生产生物反应器中的细胞活力的方法
CN201980031298.3A Active CN112136045B (zh) 2018-03-16 2019-03-15 提高生产生物反应器中的细胞活力的方法

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US (2) US11391725B2 (https=)
EP (2) EP3765846B1 (https=)
JP (2) JP7816863B2 (https=)
KR (2) KR102757905B1 (https=)
CN (2) CN119709931A (https=)
AU (1) AU2019236246B2 (https=)
BR (1) BR112020018823A2 (https=)
CA (1) CA3094076C (https=)
ES (1) ES3009097T3 (https=)
HU (1) HUE069711T2 (https=)
IL (2) IL319295A (https=)
MA (1) MA52007A (https=)
MX (2) MX2020009605A (https=)
PL (1) PL3765846T3 (https=)
SG (1) SG11202009047TA (https=)
TW (2) TWI835779B (https=)
WO (1) WO2019178508A1 (https=)
ZA (2) ZA202005741B (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4410957A4 (en) * 2021-09-30 2026-01-28 Fujifilm Corp CELL CULTURE PROCESS AND PROCESS FOR PRODUCING USEFUL SUBSTANCE
EP4594462A1 (en) 2022-09-28 2025-08-06 Genzyme Corporation Systems and devices for producing biological products and methods of using the same
US20240263122A1 (en) * 2023-02-08 2024-08-08 National Central University Bioreactor apparatus, ion-exchange substrate and method for regulation of ion concentration and cell culture thereof

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5563068A (en) * 1994-04-21 1996-10-08 Genetic Therapy, Inc. Bioreactor
WO2006089002A2 (en) * 2005-02-15 2006-08-24 Yale University Method for high throughput screening for antibodies and proteins inducing apoptosis
AU2007294731B2 (en) * 2006-09-13 2014-04-17 Abbvie Inc. Cell culture improvements
EP2500416A1 (en) * 2006-09-13 2012-09-19 Abbott Laboratories Cell culture improvements
AU2008246928B2 (en) * 2007-05-07 2014-04-17 Protalix Ltd. Large scale disposable bioreactor
WO2010120514A2 (en) 2009-03-31 2010-10-21 The Trustees Of The University Of Pennsylvania Antigen-binding proteins comprising recombinant protein scaffolds
CN104962539B (zh) * 2009-07-31 2019-09-17 百深公司 用于adamts蛋白表达的细胞培养基
EP2943563A1 (en) * 2013-01-10 2015-11-18 Biogen MA Inc. Medium supplements for improved process performance
SG10201806083RA (en) * 2013-02-22 2018-08-30 Genzyme Corp Microcarrier perfusion culturing methods and uses thereof
WO2014151901A1 (en) * 2013-03-14 2014-09-25 Abbvie Inc. Improvement of mammalian cell culture performance through surfactant supplementation of feed media
CA2905180C (en) * 2013-03-15 2023-03-07 Alder Biopharmaceuticals, Inc. Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
EP3003325A4 (en) * 2013-05-29 2017-01-11 Biogen MA Inc. Methods of evaluating cell culture additives
BR112016017660B1 (pt) * 2014-02-27 2022-04-19 F. Hoffmann-La Roche Ag Método para a produção de uma glicoproteína recombinante
SG10202001034PA (en) * 2014-12-22 2020-04-29 Genzyme Corp Methods of culturing a mammalian cell
TWI541353B (zh) * 2014-12-25 2016-07-11 財團法人工業技術研究院 產生嗜熱性鹼性蛋白酶生產菌屬（Ｔｅｐｉｄｉｍｏｎａｓ）之胞外蛋白的方法
CN108350440A (zh) * 2015-08-17 2018-07-31 阿雷克森制药公司 碱性磷酸酯的制造

Also Published As

Publication number Publication date
CN112136045A (zh) 2020-12-25
IL277352A (en) 2020-11-30
AU2019236246A1 (en) 2020-11-12
EP3765846B1 (en) 2024-10-30
BR112020018823A2 (pt) 2021-02-09
WO2019178508A1 (en) 2019-09-19
TWI835779B (zh) 2024-03-21
JP7816863B2 (ja) 2026-02-18
IL277352B1 (en) 2025-04-01
CA3094076A1 (en) 2019-09-19
EP3765846A1 (en) 2021-01-20
EP3765846C0 (en) 2024-10-30
KR102757905B1 (ko) 2025-01-22
MA52007A (fr) 2021-01-20
IL277352B2 (en) 2025-08-01
ZA202005741B (en) 2023-04-26
US11391725B2 (en) 2022-07-19
KR20250016476A (ko) 2025-02-03
PL3765846T3 (pl) 2025-03-31
EP4512887A2 (en) 2025-02-26
IL319295A (en) 2025-04-01
CN112136045B (zh) 2024-12-13
JP2024023489A (ja) 2024-02-21
JP2021515584A (ja) 2021-06-24
TW202513790A (zh) 2025-04-01
AU2019236246B2 (en) 2025-06-05
US20220308042A1 (en) 2022-09-29
MX2020009605A (es) 2021-01-08
ES3009097T3 (en) 2025-03-26
EP4512887A3 (en) 2025-04-30
SG11202009047TA (en) 2020-10-29
HUE069711T2 (hu) 2025-04-28
MX2024009056A (es) 2024-07-29
US20190285617A1 (en) 2019-09-19
TW201947033A (zh) 2019-12-16
CA3094076C (en) 2025-11-25
RU2020133815A (ru) 2022-04-19
ZA202208613B (en) 2025-01-29
KR20200132932A (ko) 2020-11-25

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