TWI732272B - Manufacturing method for an external skin used essence and a bio cellulose membrane comprising the said essence - Google Patents

Abstract

A manufacturing method for an external skin used essence and a bio cellulose membrane comprising the said essence. The method comprising the following steps of: preparing a stock solution of the essence; placing the stock solution into a vacuum homogenizer for vacuum homogenization to obtain a first process material, mixing the first process material and a catalytic enzyme and placing the mixture into a ultra-high pressure treatment device for ultra-high pressure catalysis to obtain a second process material; adding the second process material into a high-pressure homogenizer for high-pressure homogenization to obtain the essence. The said essence is added into a bio cellulose dressing to obtain a bio cellulose membrane.

Description

Preparation method of skin essence for external use and biocellulose film containing the skin essence for external use

The invention belongs to the technical field of membrane preparation, and specifically relates to a method for preparing a skin essence for external use and a biocellulose membrane containing the skin essence for external use.

The skin is the largest organ of the human body, divided into epidermis, dermis and subcutaneous tissue. Among them, the skin epidermis can be divided into stratum corneum, transparent layer, granular layer, spinous layer and basal layer from outside to inside. The outermost layer of the skin epidermis is the stratum corneum. After 14 days, the cells derived from the basal layer of the epidermis will be pushed under the stratum corneum by the continuously differentiated cells, and then gradually keratinized and become the dead stratum corneum. Naturally, the keratin components are mainly protein derivatives. The stratum corneum itself absorbs water, but because of its tight arrangement, it can prevent water from entering and escaping the epidermis. The so-called moisturizing function is to prevent the evaporation of water and allow the stratum corneum to maintain proper humidity and softness. Due to the water absorption and barrier function of the stratum corneum, as well as the natural moisturizing factors contained in the stratum corneum (ie amino acids, lactate and sugars, etc.), the stratum corneum can maintain a certain amount of water and maintain skin moisturization. The appearance of the skin is related to the moisture content of the stratum corneum. Normal skin stratum corneum usually contains 10%-30% of water to maintain the softness and elasticity of the skin. But with age, the water retention capacity of the stratum corneum of the skin will gradually decrease, and the moisture content of the stratum corneum will gradually decrease. When the moisture content of the stratum corneum of the skin is less than 10%, the skin will appear dry, tight and rough. And desquamation and other phenomena. Therefore, moisturizing is the main requirement of maintenance cosmetics.

Membrane is a common cosmetics for beauty and maintenance. It uses a short period of time covering the face to temporarily isolate the outside air and pollution, increase the skin temperature, expand the skin pores, promote sweat gland secretion and metabolism, and increase the oxygen content of the skin. The rise helps the skin to eliminate the products of epidermal cell metabolism and accumulated oils. The moisture in the membrane penetrates into the stratum corneum of the epidermis, making the skin soft and naturally bright and elastic. In the existing film packaging, the essence is often used to soak the film paper (dressing material). When the film paper covers the face, the face can absorb the effective ingredients of the essence.

However, the existing essences are generally made by traditional vacuum emulsifiers, and their emulsified particle size mostly stays in the sub-nanometre. Without special treatment by the equipment, the active substances in the formula can only stay on the surface of the stratum corneum. In addition, the existing non-woven dressings are synthetic fibers. In the bleaching process of woven fabrics, hydrogen peroxide is inevitably added for bleaching, and fluorescent brighteners are added, which is easy to cause fluorescent agents. It reacts and causes skin allergies, long-term use can cause skin damage, and its wearability is average, and it is easy to cause the dressing liquid to dry out and fall off.

The purpose of the present invention is to provide a method for preparing a skin essence for external use and a biocellulose membrane containing the skin essence for external use, which is processed through the steps of vacuum homogenization, ultra-high pressure equipment and high pressure homogenizer, so that the overall particle size of the essence tends to Rice grade can be better absorbed by the stratum corneum of the skin to achieve the effects of anti-oxidation and moisturizing the skin. With the use of biocellulose membrane dressings, the effect of the treated composition can be maximized.

In order to achieve the above-mentioned purpose, the technical solution adopted by the present invention is: a method for preparing skin essence for external use, including the following steps: step (1) first prepare the raw material liquid of the essence; step (2) place the raw material liquid in a vacuum After vacuum homogenization in the homogenizing stirring pot, the output of the primary processing process is obtained; step (3) the catalytic enzyme is added to the output of the primary processing process, and the material is placed in the ultra-high pressure treatment device for ultra-high pressure catalysis to obtain the secondary processing process output. And step (4) adding the secondary processing process to the high-pressure homogenizer for high-pressure homogenization to obtain the skin essence for external use.

In the present invention, vacuum homogenization (first processing process), ultra-high pressure treatment device catalysis (secondary processing process), and high-pressure homogenizer treatment (third processing process) are carried out in sequence. After the present invention is processed by a special ultra-high pressure processing device, the composition After the contact angle of the oil phase component of the oil phase is reduced, it can quickly penetrate into the stratum corneum cells to maximize the effectiveness of its own components. The moisturizing and moisturizing effect is strengthened. The addition of active components in the ratio can also be more effective than the traditional ratio. Effectively reduce the ratio and cost-effective. In addition, through the further processing of the high-pressure homogenizer, the overall particle size of the essence can be reduced, and it is easier to be absorbed by the stratum corneum of the skin.

Preferably, the raw material liquid in step (1) is prepared by the following steps: (a) The oil phase component of the raw material formulation, hydrogenated lecithin and natural antioxidant moisturizing composition, is heated to 50-75°C to completely dissolve it, and it is regarded as the first A mixture (Phase A) is reserved; (b) The water phase of the raw material formula is divided into glycerin, butylene glycol, diglycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, disodium EDTA, and deionized water Heat to 65～85℃ to dissolve completely, and use it as the second mixture (phase B) for later use; (c) Add the first mixture to the second mixture to obtain the raw material liquid of the essence.

Preferably, the specific operation of step (2) is: place the raw material liquid in a vacuum homogenization stirring pot, turn on the stirring blade, the stirring speed is 400-800 rpm, the homogenization speed is 2000-3000 rpm, and the vacuum is turned on to 0.02-0.06Mpa. After homogenizing and stirring for 5-15 minutes, continue to vacuum and stir to cool to 35-55℃, after venting the vacuum pressure, add colloidal silver, turn on the stirring speed of 400-800 rpm, homogenizing speed of 2000-3000 rpm, and homogenizing stirring for 2-10 minutes After that, continue to vacuum and stir to cool down to 25-45°C, and the semi-finished product is discharged and filtered and packed into barrels to obtain a one-time processing process discharge.

Preferably, the specific operation of step (3) is: adding bromelinase into the semi-finished material, put them into the composite bag together, exhaust the air in the bag and then seal, put the composite bag into the ultra-high pressure processing device, and put the composite bag into the ultra-high pressure processing device. The treatment device is filled with water as a medium. Then open the pressure control valve and open the temperature adjustment device, control the temperature at 30-45°C, adjust the pressure to 100-300 Mpa, start the isostatic pressure, and accelerate the enzymatic reaction of the inner material. After a holding time of 0.5 to 1.5 hours, adjust the pressure to 200-600 Mpa, start isostatic pressure, and accelerate the enzymatic reaction of the inner material. After the holding time of 4 to 6 hours, open the pressure relief valve and wait for the pointer to return to normal 1 atmosphere. The sealing of the composite bag is opened, and the material is discharged from the secondary processing process.

Preferably, the specific operation of step (4) is: the secondary processing process is fed into the feed port of the high-pressure homogenizer at a rate of 1 to 3 ml/sec through the peristaltic pump, and the pressure control valve and the temperature-controlled internal circulation machine are opened. , The control range of the material body pressure is 20～180 Mpa, add into the inlet in 3～5 times, the temperature inside the material body is circulated and controlled at 1～8℃; when the appearance of the material body is translucent, and the viscosity is controlled at 50～80 cps After completion, the filtered material is transported through the external circulation rotor pump to obtain the skin essence for external use of the present invention.

Preferably, the natural antioxidant moisturizing composition comprises argan tree kernel oil, cactus oil extract and avocado oil. Among them, the weight ratio of argan tree oil, cactus oil extract, and avocado oil is 1-4:0.5-2:1. The second forrest oil is produced in the desert of Morocco. It has high moisturizing and wound healing functions. It is called "desert gold" by the locals. Cactus oil extract is produced in the desert of North Africa. Due to the special structure of the growth environment, its roots go deep into the ground to ensure the water needs of growth. The seed extract oil has the function of penetrating the skin and can play a long-term moisturizing effect. Avocado oil is rich in fatty acids and vitamins A, D and E. It also has oils that can be absorbed by the epidermis. It has natural antioxidant and repair functions, and its oil can also trigger epidermal proteins to thicken the epidermis to form a complete cuticle. Floor. After being processed by the ultra-high pressure processing device, the added amount of the composition is below a very low amount, that is, a very good effect can be achieved.

Preferably, the weight percentages of each raw material are: butanediol 3.0-5.0 wt%, diglycerol 1.0-3.0 wt%, hydrogenated lecithin 1.0-2.0 wt%, subargan tree oil 1.0-2.0 wt%, cactus oil extraction 0.5-1.0 wt%, avocado oil 0.5-1.0 wt%, sodium hyaluronate 0.3-0.5 wt%, allantoin 0.15-0.25 wt%, dipotassium glycyrrhizinate 0.15-0.25 wt%, colloidal silver 0.1- 0.2 wt%, disodium EDTA 0.1-0.2 wt%, bromelinase 0.05-0.1 wt%, and the balance is deionized water.

The composition of each raw material of the present invention can also be selected from other mature components on the market or corresponding components disclosed in other patents.

Preferably, the weight percentage of the natural antioxidant moisturizing composition is 2.6 wt%.

Preferably, the preparation method of the skin essence for external use includes the following steps: (1) The oil phase component of the raw material formula, hydrogenated lecithin and natural anti-oxidant moisturizing composition, is heated to 65°C to completely dissolve it, which is regarded as the first Mixture (Phase A) for use; (2) Heat the water phase of the raw material formula into glycerin, butylene glycol, diglycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, disodium EDTA, and deionized water Dissolve completely at 75°C and use it as the second mixture (phase B) for later use; (3) Add the first mixture to the second mixture to obtain the raw material liquid of the essence. Put the raw material liquid in a fixed vacuum homogenization stirring pot, turn on the fixed vacuum homogenization stirring blade, the stirring speed is 600 rpm, the homogenization speed is 2500 rpm, and the vacuum is turned on to 0.04 Mpa. After homogenizing and stirring for 10 minutes, continue to vacuum and stir to cool to 45°C, after releasing the vacuum pressure, add colloidal silver, turn on the stirring speed of 600 rpm, homogenizing speed of 2500 rpm, homogenizing and stirring for 5 minutes, continue to vacuum and stir to cool down to 35°C, the semi-finished product is discharged and filtered and packed into barrels to obtain a processing process Discharge the material for use; (4) Add bromelinase into the discharging material of one processing process, and put them into the composite bag together, exhaust the air in the bag and seal it, and put the composite bag in the ultra-high pressure processing device, and the ultra-high pressure processing device Fill in water as a medium. Then open the pressure control valve, and open the temperature adjustment device, control the temperature at 37°C, adjust the pressure to 200 Mpa, start the isostatic pressure, and accelerate the enzymatic reaction of the inner material. After the maintenance time is 1 hour, adjust the pressure to 400 Mpa, the isostatic pressure is started, the inner material accelerates the enzyme reaction, and the pressure relief valve is opened after the maintenance time is 5 hours. When the pointer returns to normal 1 atmosphere, the composite bag seal is opened, and the result is The secondary processing process discharges the material for standby; and (5) The secondary processing process discharges the 2 ml/sec quantitatively into the high pressure homogenizer inlet through the peristaltic pump, open the pressure control valve and the temperature control internal circulation machine, the material body pressure Control 100 Mpa and add it to the inlet in 4 times. The temperature inside the material is circulated at 3～5℃; when the appearance of the material is translucent, the viscosity is controlled at 65 cps, and then it is filtered out through the external circulation rotor pump. Material to obtain the skin essence of the present invention for external use.

Another object of the present invention is to provide a biocellulose membrane, wherein the above-mentioned external skin essence is added to a biocellulose membrane dressing to prepare a biocellulose membrane.

The beneficial effects of the present invention are: (1) The present invention is prepared by three processes of vacuum homogenization, ultra-high pressure catalysis, and high-pressure homogenization. The anti-oxidation and moisturizing properties of the essence are remarkable. In addition, through the secondary ultra-high pressure device processing process, The surface tension of the oil droplets of the gansu kernel oil, cactus oil extract, and avocado oil composition will change. After high-pressure treatment, the surface tension of the oil droplets of the composition will decrease, and the oil droplets will be converted from macromolecules to small molecules, and the viscosity of the oil droplets will decrease. The contact angle of the oil droplets is also reduced, the penetration force relative to the skin will be stronger, and the effect of moisturizing and moisturizing will be better; (2) When the content of the present invention is processed by ultra-high pressure, almost all the bacteria in the formula are Killing, therefore, the content of preservatives can be greatly reduced, reducing skin irritation, and the content of the composition can also be greatly reduced after ultra-high pressure treatment, compared with the content of the composition that has not been subjected to ultra-high pressure treatment. It effectively reduces the cost of the product and improves the cost-effectiveness of the product; (3) After three processing steps, the essence of the essence is put into the high-pressure homogenizer again, and the overall particle size of the essence is converted to nanometer level, which is more capable Effectively make the anti-oxidant and moisturizing active ingredients in the formula penetrate into the stratum corneum cells to maximize the effect of its own ingredients; (4) The material of the biocellulose membrane dressing is better than the general non-woven dressing, which has good elasticity and toughness. Strong, good adherence, close to the skin, active ingredients penetrate into the stratum corneum of the skin, no allergies and other advantages, safety can be effectively guaranteed.

The present invention will be further described below in conjunction with specific embodiments, but the scope of the present invention to be protected is not limited to this.

Example 1: The weight percentage composition of each raw material is: butylene glycol 4.0 wt%, diglycerol 2.0 wt%, hydrogenated lecithin 1.5 wt%, sub-Argan oil 1.2 wt%, cactus oil extract 0.7 wt%, avocado Oil 0.7 wt%, sodium hyaluronate 0.4 wt%, allantoin 0.2 wt%, dipotassium glycyrrhizinate 0.2 wt%, colloidal silver 0.15 wt%, disodium EDTA 0.15 wt%, bromelinase 0.07 wt%, balance It is deionized water.

The preparation method of the skin essence for external use includes the following steps: (1) The oil phase component hydrogenated lecithin and the natural anti-oxidant moisturizing composition of the raw material formula are heated to 65° C. to completely dissolve, as the first mixture (A (2) The water phase of the raw material formula consists of glycerin, butylene glycol, diglycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, disodium EDTA, deionized water and heat to 75℃ completely Dissolve it and treat it as the second mixture (phase B) for later use; (3) Add the first mixture to the second mixture to obtain the raw material liquid of the essence, and then place the raw material liquid in a fixed vacuum homogenization stirring pot, and turn on the fixed Vacuum homogenizing mixing blade, mixing speed 600 rpm, homogenizing speed 250 0rpm, turn on the vacuum to 0.04 Mpa, after homogenizing stirring for 10 minutes, continue to vacuum and stir to cool down to 45℃, after venting the vacuum pressure, add colloidal silver and turn on the stirring Speed of 600 rpm, homogenizing speed of 2500 rpm, after homogenizing and stirring for 5 minutes, continue to vacuum and stir to cool to 35°C, the semi-finished product is discharged and filtered and packed into barrels to obtain the discharge of one processing process, which is reserved; The pineapple protease is put into a composite bag together, the air in the bag is exhausted and then sealed, the composite bag is put into an ultra-high pressure treatment device, and the ultra-high pressure treatment device is filled with water as a medium. Then open the pressure control valve and open the temperature adjustment device, control the temperature at 37°C, adjust the pressure to 200Mpa, start the isostatic pressure, and accelerate the enzymatic reaction of the inner material. After the maintenance time is 1 hour, adjust the pressure to 400Mpa, the isostatic pressure is started, the inner material accelerates the enzyme reaction, and the pressure relief valve is opened after the maintenance time is 5 hours. When the pointer returns to normal 1 atmosphere, the composite bag seal is opened, and the result is two The secondary processing process discharges the material for use; (5) The secondary processing process discharges 2ml/sec quantitatively into the high pressure homogenizer inlet through the peristaltic pump, open the pressure control valve and the temperature control internal circulation machine, the material pressure is controlled at 100Mpa, Add it to the inlet in 4 times, and control the temperature inside the material body at 3～5℃; when the appearance of the material body is translucent, the viscosity is controlled at 65cps, and then the material is filtered and discharged through the external circulation rotor pump, and the skin is obtained. Essence for external use.

The particle size detection of the final product: Use the Malvern laser micrometer to test the data. The laser micrometer test results are shown in Table 1:

Table 1 Run (number of times) Angle KCps value ZAve (average z value) 1 90 254 129.4 2 90 255.1 117.6 3 90 254.8 115.3 Average 254.6 120.8 +/- 0.6 7.6

From the data in Table 1, it is known that the average fineness of the microemulsified skin essence for external use provided by the present invention is 120.8 nm; the positive and negative deviation is 7.6 nm.

In the evaluation project of penetration into skin and hair, surface tension and wetness are often used as test indicators. Generally speaking, if the surface tension becomes smaller, it will be easier to wet, which means that the penetration into the skin and hair will also increase. The present invention uses the degree of wettability as an indicator, and uses a contact angle that can be widely used in all areas. The smaller the contact angle, the easier it is to wet.

Using a contact angle measuring device to measure the contact angle of the skin essence for external use, the contact angle of the essence of the present invention reached 17.2 degrees, while the contact angle of the product without the high-pressure homogenization treatment of the ultra-high pressure catalyst was 27.3 degrees, so it can be confirmed that the present invention requested The preparation method of Essence can significantly improve the penetration of the essence to the skin, and it has better penetration.

In order to understand the effects of the present invention, extensive trial investigations were conducted on the skin essence for external use in the form of a questionnaire survey. The survey included a total of 860 male and female trial users from all over the country, including 160 males and 700 females, the oldest being 52 years old. , The youngest is 25 years old, the average age is about 38 years old, and a total of 680 valid questionnaires have been collected.

After 56 days of using the skin essence for external use prepared by the preparation method requested by the present invention, 680 testers conducted self-evaluation on the levels of relevant indicators. The results are as follows: Increased skin moisturizing: 76.2% and 86.8%, respectively Trial users have moderate improvement and obvious improvement; Improve skin brightness: 75.3% and 82.4% of trial users have moderate improvement and obvious improvement; Weaken skin fine lines: 62.4% and 77.8% of trials Those who have moderate improvement and obvious improvement. It can be seen from the self-assessment results of the testers that the external skin essence prepared by the preparation method of the present invention can increase skin moisturization, enhance skin brightness, and weaken skin fine lines. .

According to the above results, it is shown that the optimal component ratio of the external skin essence of the present invention combined with the ultra-high pressure equipment and the high-pressure homogenizer-assisted nano-preparation method can achieve good anti-oxidation and moisturizing effects.

The above-mentioned skin essence for external use is added to the biocellulose membrane dressing to prepare a biocellulose membrane. Biocellulose membrane dressings can be purchased from mature materials on the market, or they can be prepared using Chinese patents CN201710505913, CN201810618623, CN201810976359 and other public preparation methods. Biocellulose membrane dressing is a product of microbial fermentation. It can be used instead of artificial skin in the medical field. It has good skin safety, and its adherence is better than that of non-woven dressing substrate, and it is not easy to produce dressing liquid to dry out and fall off. The situation happened.

Example 2: The weight percentage composition of each raw material is: Butylene glycol 3.0 wt%, Diglycerol 1.5 wt%, Hydrogenated lecithin 1.0 wt%, Argan oil 1.0 wt%, Cactus oil extract 1.0 wt%, Avocado Oil 1.0 wt%, sodium hyaluronate 0.3 wt%, allantoin 0.15 wt%, dipotassium glycyrrhizinate 0.15 wt%, colloidal silver 0.1 wt%, disodium EDTA 0.1 wt%, bromelinase 0.05 wt%, balance It is deionized water.

The preparation method of the skin essence for external use includes the following steps: (1) The oil phase component hydrogenated lecithin and the natural anti-oxidant moisturizing composition of the raw material formula are heated to 65° C. to completely dissolve, as the first mixture (A (2) The water phase of the raw material formula consists of glycerin, butylene glycol, diglycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, disodium EDTA, deionized water and heat to 75℃ completely Dissolve it and treat it as the second mixture (phase B) for later use; (3) Add the first mixture to the second mixture to obtain the raw material liquid, place the raw material liquid in the stationary vacuum homogenizing stirring pot, and turn on the stationary vacuum Homogeneous stirring blade, stirring speed 600 rpm, homogenizing speed 2500 rpm, turn on the vacuum to 0.04 Mpa, after 10 minutes of homogeneous stirring, continue to vacuum and stir to cool to 45°C, after venting the vacuum pressure, add colloidal silver and turn on the stirring speed at 600 rpm , The homogenizing speed is 2500 rpm, after homogenizing and stirring for 5 minutes, continue to vacuum and stir to reduce the temperature to 35℃, the semi-finished product is discharged and filtered and packed into barrels for later use; (4) Add bromelinase to the semi-finished material, put them into the composite bag together, and drain the bag After air is sealed, the composite bag is put into the ultra-high pressure processing device, and the ultra-high pressure processing device is filled with water as the medium. Then open the pressure control valve, and open the temperature adjustment device, control the temperature at 37°C, adjust the pressure to 200 Mpa, start the isostatic pressure, and accelerate the enzymatic reaction of the inner material. After the maintenance time is 1 hour, adjust the pressure to 400 Mpa, the isostatic pressure is started, the inner material accelerates the enzyme reaction, and the pressure relief valve is opened after the maintenance time is 5 hours. When the pointer returns to normal 1 atmosphere, the composite bag seal is opened for standby; (5) The material in the composite bag is added to the inlet of the high-pressure homogenizer through a peristaltic pump at a rate of 2 ml/sec. The pressure control valve and the temperature control internal circulation machine are turned on. The pressure of the material is controlled to 100 Mpa, and the material is added in 4 times. The temperature inside the material is controlled at 3～5℃; when the appearance of the material is translucent, the viscosity is controlled at 65 cps, and then the material is filtered and discharged through the external circulation rotor pump to obtain the skin essence for external use.

The particle size detection of the final product: Using the Malvern laser micrometer to test the data, the laser micrometer test shows that the average fineness of the microemulsion essence provided by the invention is 145.3 nm.

The contact angle of the skin essence for external use was measured by a contact angle measuring device. The contact angle of the essence of the present invention reached 19.1 degrees, while the contact angle of the product without ultra-high pressure catalyst high pressure homogenization treatment was 28.6 degrees, so it can be confirmed that the present invention requested The preparation method of Essence can significantly improve the penetration of the essence to the skin, and it has better penetration.

In order to understand the effects of the present invention, an extensive trial survey was conducted on this product in the form of a questionnaire survey. The survey included a total of 860 male and female trial users from all over the country, including 160 males and 700 females, the oldest being 52 years old, and the youngest being 25. Years old, with an average age of about 38 years old, a total of 680 valid questionnaires were collected.

After 56 days of using the skin essence for external use prepared by the preparation method requested by the present invention, 680 testers conducted self-evaluation on the relevant index levels. The results are as follows: Increased skin moisturizing: 73.1% and 82.2%, respectively Trial users have moderate improvement and obvious improvement; Improve skin brightness: 72.1% and 80.1% of trial users have moderate improvement and obvious improvement; Weaken skin fine lines: 60.2% and 70.6% of trials respectively It can be seen from the self-assessment results of the testers that the product can increase skin moisturization, enhance skin brightness, and weaken skin fine lines.

The above-mentioned skin essence for external use is added to the biocellulose membrane dressing to prepare a biocellulose membrane. Biocellulose membrane dressings can be purchased from mature materials on the market, or they can be prepared by using Chinese patents CN201710505913, CN201810618623, CN201810976359 and other public preparation methods. Biocellulose membrane dressing is a product of microbial fermentation. It can be used instead of artificial skin in the medical field. It has good skin safety, and its adherence is better than that of non-woven dressing substrate, and it is not easy to produce dressing liquid to dry out and fall off. The situation happened.

Example 3: The weight percentage composition of each raw material is: butylene glycol 5.0 wt%, diglycerol 2.5 wt%, hydrogenated lecithin 1.8 wt%, subargan oil 1.8 wt%, cactus oil extract 0.8 wt%, avocado Oil 0.8 wt%, sodium hyaluronate 0.4 wt%, allantoin 0.2 wt%, dipotassium glycyrrhizinate 0.2 wt%, colloidal silver 0.15 wt%, disodium EDTA 0.15 wt%, pineapple protease 0.08 wt%, balance It is deionized water.

The preparation method of the skin essence for external use includes the following steps: (1) The oil phase component hydrogenated lecithin and the natural anti-oxidant moisturizing composition of the raw material formula are heated to 65° C. to completely dissolve, as the first mixture (A (2) The water phase of the raw material formula consists of glycerin, butylene glycol, diglycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, disodium EDTA, and deionized water, and heat to 75°C completely Dissolve it and treat it as the second mixture (phase B) for later use; (3) Add the first mixture to the second mixture to obtain the raw material liquid of the essence, and then place the raw material liquid in a fixed vacuum homogenization stirring pot, and turn on the fixed Homogenizing mixing blade with vacuum, stirring speed of 600 rpm, homogenizing speed of 2500 rpm, turn on the vacuum to 0.04 Mpa, after 10 minutes of homogenization, continue to vacuum and stir to cool down to 45℃, after venting the vacuum pressure, add colloidal silver and turn on the stirring Speed 600 rpm, homogenizing speed 2500 rpm, after homogenizing and stirring for 5 minutes, continue to vacuum and stir to cool down to 35℃, the semi-finished product is discharged, filtered and packed into barrels for later use; (4) Add bromelinase to the semi-finished material, put it into a composite bag together, and pump After the air in the bag is sealed, the composite bag is put into the ultra-high pressure processing device, and the ultra-high pressure processing device is filled with water as the medium. Then open the pressure control valve, and open the temperature adjustment device, control the temperature at 37°C, adjust the pressure to 200 Mpa, start the isostatic pressure, and accelerate the enzymatic reaction of the inner material. After the maintenance time is 1 hour, adjust the pressure to 400 Mpa, the isostatic pressure is started, the inner material accelerates the enzyme reaction, and the pressure relief valve is opened after the maintenance time is 5 hours. When the pointer returns to normal 1 atmosphere, the composite bag seal is opened for standby; (5) The material in the composite bag is added to the inlet of the high-pressure homogenizer through a peristaltic pump at a rate of 2 ml/sec. The pressure control valve and the temperature control internal circulation machine are turned on. The pressure of the material is controlled to 100 Mpa, and the material is added in 4 times. The temperature inside the material is controlled at 3～5℃; when the appearance of the material is translucent, the viscosity is controlled at 65 cps, and then the material is filtered and discharged through the external circulation rotor pump to obtain the skin essence for external use.

The particle size detection of the final product: Using the Malvern laser micrometer to test the data, the laser micrometer test shows that the average fineness of the microemulsion essence provided by the invention is 136.8 nm.

Using a contact angle measuring device to measure the contact angle of the skin essence for external use, the contact angle of the essence of the present invention reached 18.4 degrees, while the contact angle of the product without the high pressure homogenization treatment of the ultra-high pressure catalyst was 27.8 degrees, so it can be confirmed that the present invention requested The preparation method of Essence can significantly improve the penetration of the essence to the skin, and it has better penetration.

In order to understand the effects of the present invention, an extensive trial survey was conducted on this product in the form of a questionnaire survey. The survey included a total of 860 male and female trial users from all over the country, including 160 males and 700 females, the oldest being 52 years old, and the youngest being 25. Years old, with an average age of about 38 years old, a total of 680 valid questionnaires were collected.

After 56 days of using the skin essence for external use prepared by the preparation method requested by the present invention, 680 testers conducted self-evaluation on the degree of relevant indicators. The results are as follows: Increased skin moisturization: 74.2% and 84.2%, respectively The testers of the tester have moderate improvement and obvious improvement; enhance the skin brightness: 73.5% and 81.6% of the testers have moderate and obvious improvement; weaken the skin fine lines: 61.3% and 74.9% of the testers It can be seen from the self-assessment results of the testers that the product can increase skin moisturization, enhance skin brightness, and weaken skin fine lines.

The above-mentioned skin essence for external use is added to the biocellulose membrane dressing to prepare a biocellulose membrane. Biocellulose membrane dressings can be purchased from mature materials on the market, or they can be prepared by using Chinese patents CN201710505913, CN201810618623, CN201810976359 and other public preparation methods. Biocellulose membrane dressing is a product of microbial fermentation. It can be used instead of artificial skin in the medical field. It has good skin safety, and its adherence is better than that of non-woven dressing substrate, and it is not easy to produce dressing liquid to dry out and fall off. The situation happened.

Example 4: The weight percentage composition of each raw material is: Butylene glycol 4.0 wt%, Diglycerol 2.0%, Hydrogenated lecithin 1.5 wt%, Argan oil 1.2 wt%, Cactus oil extract 0.7 wt%, Avocado oil 0.7 wt%, sodium hyaluronate 0.4 wt%, allantoin 0.20 wt%, dipotassium glycyrrhizinate 0.2 wt%, colloidal silver 0.15 wt%, disodium EDTA 0.15 wt%, ananasase 0.07 wt%, the balance is Deionized water.

The preparation method of the skin essence for external use includes the following steps: (1) The oil phase component hydrogenated lecithin and the natural anti-oxidant moisturizing composition of the raw material formula are heated to 50° C. to completely dissolve, as the first mixture (A (2) The water phase of the raw material formula is composed of glycerin, butylene glycol, diglycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, disodium EDTA, and deionized water. Dissolve it and treat it as the second mixture (phase B) for later use; (3) Add the first mixture to the second mixture to obtain the raw material liquid of the essence, and then place the raw material liquid in a fixed vacuum homogenization stirring pot, and turn on the fixed Homogenizing mixing blade with vacuum, stirring speed of 400 rpm, homogenizing speed of 2000 rpm, turn on the vacuum to 0.02 Mpa, after homogenizing and stirring for 5 minutes, continue to vacuum and stir to cool to 35°C, after venting the vacuum pressure, add colloidal silver and turn on Stirring speed 400 rpm, homogenizing speed 2000 rpm, after homogenizing stirring for 2 minutes, continue to vacuum and stir to cool down to 25℃, the semi-finished product is discharged and filtered and packed into barrels for later use; (4) Add bromelinase to the semi-finished material and put it into a composite bag together. After evacuating the air in the bag and sealing it, the composite bag is put into the ultra-high pressure processing device, and the ultra-high pressure processing device is filled with water as the medium. Open the pressure control valve, and open the temperature adjustment device, control the temperature at 30°C, adjust the pressure to 100 Mpa, start the isostatic pressure, and accelerate the enzymatic reaction of the inner material. After the maintenance time is 1.5 hours, adjust the pressure to 200Mpa, the isostatic pressure is started, the inner material accelerates the enzyme reaction, after the maintenance time is 6 hours, open the pressure relief valve, when the pointer returns to normal 1 atmosphere, the composite bag seal is opened for standby; 5) The material in the composite bag is added to the inlet of the high-pressure homogenizer at a rate of 1 ml/sec through the peristaltic pump. The pressure control valve and the temperature-controlled internal circulation machine are turned on. The pressure of the material is controlled at 20 Mpa and added to the inlet in 5 times. , The temperature inside the material is controlled at 5～7℃; when the appearance of the material is translucent, the viscosity is controlled at 50 cps, and then the material is filtered and discharged through the external circulation rotor pump to obtain the skin essence for external use.

The particle size detection of the final product: Using the Malvern laser micrometer test data, the laser micrometer test found that the average fineness of the microemulsion essence provided by the invention is 192.3 nm.

Using a contact angle measuring device to measure the contact angle of the essence for external use on the skin, the contact angle of the essence of the present invention reached 20.1 degrees, so it can be confirmed that the present invention has good penetration to the skin.

In order to understand the effects of the present invention, an extensive trial survey was conducted on this product in the form of a questionnaire survey. The survey included a total of 860 male and female trial users from all over the country, including 160 males and 700 females, the oldest being 52 years old, and the youngest being 25. Years old, with an average age of about 38 years old, a total of 680 valid questionnaires were collected.

After 56 days of using the skin essence for external use prepared by the preparation method requested by the present invention, 680 testers conducted self-evaluation on the levels of relevant indicators. The results are as follows: Increased skin moisturization: 70.1% and 80.2%, respectively Trial users have moderate and obvious improvement; improve skin brightness: 70.2% and 80.1% of trial users have moderate and significant improvement; weaken skin fine lines: 60.2% and 70.1% of trial It can be seen from the self-assessment results of the testers that the product can increase skin moisturization, enhance skin brightness, and weaken skin fine lines.

The above-mentioned skin essence for external use is added to the biocellulose membrane dressing to prepare a biocellulose membrane. Biocellulose membrane dressings can be purchased from mature materials on the market, or they can be prepared by using Chinese patents CN201710505913, CN201810618623, CN201810976359 and other public preparation methods. Biocellulose membrane dressing is a product of microbial fermentation. It can be used instead of artificial skin in the medical field. It has good skin safety, and its adherence is better than that of non-woven dressing substrate, and it is not easy to produce dressing liquid to dry out and fall off. The situation happened.

Example 5: The weight percentage composition of each raw material is: butanediol 4.0 wt%, diglycerol 2.0 wt%, hydrogenated lecithin 1.5 wt%, subargan oil 1.2 wt%, cactus oil extract 0.7 wt%, avocado Oil 0.7 wt%, sodium hyaluronate 0.4 wt%, allantoin 0.20 wt%, dipotassium glycyrrhizinate 0.2 wt%, colloidal silver 0.15 wt%, disodium EDTA 0.15 wt%, bromelinase 0.07 wt%, balance It is deionized water.

The preparation method of the skin essence for external use includes the following steps: (1) The oil phase component hydrogenated lecithin and the natural anti-oxidant moisturizing composition of the raw material formula are heated to 75° C. to completely dissolve, as the first mixture (A (2) The water phase of the raw material formula consists of glycerin, butylene glycol, diglycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, disodium EDTA, deionized water and heat to 85°C completely Dissolve it and treat it as the second mixture (phase B) for later use; (3) Add the first mixture to the second mixture to obtain the raw material liquid of the essence, and then place the raw material liquid in a fixed vacuum homogenization stirring pot, and turn on the fixed Vacuum homogenizing mixing blade, mixing speed 800 rpm, homogenizing speed 3000 rpm, turn on the vacuum to 0.06 Mpa, after 15 minutes of homogeneous stirring, continue to vacuum and stir to cool to 55°C, after venting the vacuum pressure, add colloidal silver and turn on the stirring The speed is 800 rpm, the homogenization speed is 3000 rpm. After homogenizing and stirring for 10 minutes, continue to vacuum and stir to cool down to 45°C. The semi-finished material is discharged and filtered and packed into barrels for later use; (4) Add bromelinase to the semi-finished material and put it into a composite bag together. After the air in the bag is sealed, the composite bag is put into the ultra-high pressure processing device, and the ultra-high pressure processing device is filled with water as the medium. Then open the pressure control valve, and open the temperature adjustment device, control the temperature at 45°C, adjust the pressure to 300 Mpa, start the isostatic pressure, and accelerate the enzymatic reaction of the inner material. After the holding time is 0.5 hours, adjust the pressure to 600 Mpa, the isostatic pressure is started, the inner material accelerates the enzyme reaction, and the pressure relief valve is opened after the holding time is 4 hours. When the pointer returns to normal 1 atmosphere, the composite bag seal is opened for standby; (5) The material in the composite bag is fed into the feed port of the high-pressure homogenizer at a rate of 3 ml/sec through the peristaltic pump, and the pressure control valve and temperature control internal circulation machine are turned on. The material pressure is controlled at 180 Mpa, and the material is added in 3 times. The temperature inside the material is controlled at 1～3℃; when the appearance of the material is translucent, the viscosity is controlled at 80 cps, and then the material is filtered and discharged through the external circulation rotor pump to obtain the skin essence for external use.

The particle size detection of the final product: Using the Malvern laser micrometer to test the data, the laser micrometer test shows that the average fineness of the microemulsion essence provided by the invention is 187.6 nm.

Using a contact angle measuring device to measure the contact angle of the essence for external use on the skin, the contact angle of the essence of the present invention reached 19.6 degrees, which confirms that the present invention has good penetration to the skin.

In order to understand the effects of the present invention, an extensive trial survey was conducted on this product in the form of a questionnaire survey. The survey included a total of 860 male and female trial users from all over the country, including 160 males and 700 females, the oldest being 52 years old, and the youngest being 25. Years old, with an average age of about 38 years old, a total of 680 valid questionnaires were collected.

After 56 days of using the skin essence for external use prepared by the preparation method requested by the present invention, 680 testers conducted self-evaluation on the levels of relevant indicators. The results are as follows: Increased skin moisturizing: 71.3% and 81.1%, respectively Trial users have moderate improvement and obvious improvement; Improve skin brightness: 70.9% and 81.2% of trial users have moderate improvement and obvious improvement; Weaken skin fine lines: 61.1% and 71.2% of trial It can be seen from the self-assessment results of the testers that the product can increase skin moisturization, enhance skin brightness, and weaken skin fine lines.

The above-mentioned skin essence for external use is added to the biocellulose membrane dressing to prepare a biocellulose membrane. Biocellulose membrane dressings can be purchased from mature materials on the market, or they can be prepared by using Chinese patents CN201710505913, CN201810618623, CN201810976359 and other public preparation methods. Biocellulose membrane dressing is a product of microbial fermentation. It can be used instead of artificial skin in the medical field. It has good skin safety, and its adherence is better than that of non-woven dressing substrate, and it is not easy to produce dressing liquid to dry out and fall off. The situation happened.

Example 6: The weight percentage composition of each raw material is: butanediol 4.0 wt%, diglycerin 2.5 wt%, hydrogenated lecithin 1.5 wt%, subargan oil 1.5 wt%, cactus oil extract 0.8 wt%, avocado Oil 0.9 wt%, sodium hyaluronate 0.4 wt%, allantoin 0.20 wt%, dipotassium glycyrrhizinate 0.2 wt%, colloidal silver 0.15 wt%, disodium EDTA 0.15 wt%, bromelinase 0.06 wt%, balance It is deionized water.

The preparation method of the skin essence for external use includes the following steps: (1) The oil phase component hydrogenated lecithin and the natural anti-oxidant moisturizing composition of the raw material formula are heated to 60° C. to completely dissolve, and the mixture is regarded as the first mixture (A (2) The water phase of the raw material formula consists of glycerin, butylene glycol, diglycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, disodium EDTA, and deionized water, heated to 70℃ completely Dissolve it and treat it as the second mixture (phase B) for later use; (3) Add the first mixture to the second mixture to obtain the raw material liquid, place the raw material liquid in the stationary vacuum homogenizing stirring pot, and turn on the stationary vacuum Homogeneous stirring blade, stirring speed 500 rpm, homogenizing speed 2200 rpm, turn on the vacuum to 0.04 Mpa, after 10 minutes of homogeneous stirring, continue to vacuum and stir to cool down to 40℃, after venting the vacuum pressure, add colloidal silver and turn on the stirring speed at 500 rpm , The homogenizing speed is 2200 rpm, after homogenizing and stirring for 5 minutes, continue to vacuum and stir to reduce the temperature to 30℃, the semi-finished product is discharged, filtered and packed into barrels for later use; (4) Add bromelain to the semi-finished material, put them into a composite bag together, and then light the bag After air is sealed, the composite bag is put into the ultra-high pressure processing device, and the ultra-high pressure processing device is filled with water as the medium. Then open the pressure control valve and open the temperature adjustment device, control the temperature at 35°C, adjust the pressure to 250 Mpa, start the isostatic pressure, and accelerate the enzymatic reaction of the inner material. After the maintenance time is 1 hour, adjust the pressure to 450 Mpa, the isostatic pressure starts, and the inner material accelerates the enzyme reaction. After the maintenance time is 5 hours, open the pressure relief valve, and when the pointer returns to normal 1 atmosphere, the composite bag seal is opened for standby; (5) The material in the composite bag is added to the inlet of the high-pressure homogenizer at a rate of 2.5 ml/sec through a peristaltic pump. , Open the pressure control valve and the temperature-controlled internal circulation machine, the material pressure is controlled at 120 Mpa, and the material is added to the inlet in 4 times. The temperature of the material is circulated at 3～5℃; the appearance of the material is translucent, and the viscosity is controlled at It is completed at 60 cps, and then the filtered material is transported through the external circulation rotor pump to obtain the skin essence for external use.

The particle size detection of the final product: using the Malvern laser micronometer to test the data, the laser micronometer test shows that the average fineness of the microemulsion essence provided by the present invention is 133.6 nm.

Using a contact angle measuring device to measure the contact angle of the essence for external use on the skin, the contact angle of the essence of the present invention reached 18.1 degrees, so it can be confirmed that the present invention has good penetration to the skin.

In order to understand the effects of the present invention, an extensive trial survey was conducted on this product in the form of a questionnaire survey. The survey included a total of 860 male and female trial users from all over the country, including 160 males and 700 females, the oldest being 52 years old, and the youngest being 25. Years old, with an average age of about 38 years old, a total of 680 valid questionnaires were collected.

After 56 days of using the skin essence for external use prepared by the preparation method requested by the present invention, 680 testers conducted self-evaluation on the relevant index levels. The results are as follows: Increased skin moisturizing: 75.1% and 85.2%, respectively Trial users have moderate improvement and obvious improvement; Improve skin brightness: 74.3% and 82.1% of trial users have moderate improvement and obvious improvement; Weaken skin fine lines: 61.9% and 75.2% of trials respectively It can be seen from the self-assessment results of the testers that the product can increase skin moisturization, enhance skin brightness, and weaken skin fine lines.

The above-mentioned skin essence for external use is added to the biocellulose membrane dressing to prepare a biocellulose membrane. Biocellulose membrane dressings can be purchased from mature materials on the market, or they can be prepared by using Chinese patents CN201710505913, CN201810618623, CN201810976359 and other public preparation methods. Biocellulose membrane dressing is a product of microbial fermentation. It can be used instead of artificial skin in the medical field. It has good skin safety, and its adherence is better than that of non-woven dressing substrate, and it is not easy to produce dressing liquid to dry out and fall off. The situation happened.

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Claims (9)

A method for preparing a skin essence for external use includes the following steps: preparing a raw material liquid, comprising: heating hydrogenated lecithin and a natural anti-oxidant moisturizing composition to 50~75°C to completely dissolve to obtain a first mixture. The first mixture is the oil phase composition of the raw material liquid; heat glycerin, butylene glycol, diglycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, disodium EDTA, and deionized water to 65~85 ℃ completely dissolved to obtain a second mixture, the second mixture is the water phase component of the raw material liquid; and the first mixture is added to the second mixture to obtain the raw material liquid; the raw material liquid is placed A vacuum homogenization process is carried out in a vacuum homogenization stirring pot to obtain a processing process output; an pineapple protease is added to the processing process output and placed in an ultra-high pressure treatment device to perform an ultra-high pressure catalytic process to obtain a The secondary processing process is discharged; the secondary processing process is fed into a high-pressure homogenizer to perform a high-pressure homogenization process to obtain the skin essence for external use. The preparation method of the skin essence for external use according to claim 1, wherein the step of preparing the raw material of the one-time processing process comprises: putting the raw material liquid into the vacuum homogenizing stirring pot, turning on the stirring blade, and stirring at a speed of 400-800 rpm, homogenizing Turn on the vacuum to 0.02~0.06Mpa at the speed of 2000~3000rpm, homogenize and stir for 5~15 minutes, continue to vacuum and stir to cool to 35~55℃, after the vacuum pressure is released, add a colloidal silver, and then turn on the stirring speed 400 ~800rpm, homogenization speed 2000~3000rpm, after homogenization stirring for 2~10 minutes, continue to vacuum and stir to cool down to 25~45℃, the semi-finished product is discharged and filtered and packed into barrels to obtain the discharge of this one-time processing process. The method for preparing the skin essence for external use according to claim 2, wherein the step of preparing the secondary processing process discharge material comprises: adding an pineapple protease to the primary processing process discharge material and then putting it into a composite bag, and evacuating it. After the air in the bag is sealed, the composite bag is put into the ultra-high pressure processing device, and then water is filled as the medium, the temperature is controlled at 30~45℃, the pressure is adjusted to 100~300Mpa, the isostatic pressure is started, and the internal material is accelerated. Enzyme reaction, after the maintenance time is 0.5~1.5 hours, adjust the pressure to 200~600Mpa, the isostatic pressure is started, the inner material accelerates the enzyme reaction, after the maintenance time is 4~6 hours, open a pressure relief valve, and wait for the pointer to return to normal 1 At atmospheric pressure, the composite bag is sealed and opened to obtain the material discharged from the secondary processing process. The method for preparing the skin essence for external use according to claim 3, wherein the step of obtaining the skin essence for external use further comprises: passing the secondary processing process discharge through a peristaltic pump into the high-pressure homogenizer at a quantitative rate of 1~3ml/sec In the feed port of the machine, the control range of the material body pressure is 20~180Mpa, and it is added into the feed port in 3~5 times. The temperature in the material body is circulated and controlled at 1~8℃; the appearance of the material body is translucent, and the viscosity is controlled at 50 It is completed at ~80cps, and then the filtered material is transported through an external circulation rotor pump to obtain the skin essence for external use. The preparation method of the skin essence for external use according to claim 1, wherein the natural antioxidant moisturizing composition comprises argan oil, cactus oil extract, and avocado oil, wherein, argan oil, cactus oil extract , The weight ratio of avocado oil is 1~4:0.5~2:1. According to the preparation method of the skin essence for external use according to claim 3, the weight percentage of each raw material is: butylene glycol 3.0~5.0wt%, diglycerin 1.0~3.0wt%, hydrogenated lecithin 1.0~2.0wt%, Argan tree kernel oil 1.0~2.0wt%, cactus oil extract 0.5~1.0wt%, avocado oil 0.5~1.0wt%, sodium hyaluronate 0.3~0.5wt%, allantoin 0.15~0.25wt%, glycerin Dipotassium oxalate 0.15~0.25wt%, colloidal silver 0.1~0.2wt%, EDTA disodium 0.1~0.2wt%, bromelinase 0.05~0.1wt%, the balance is deionized water. According to the preparation method of the skin essence for external use according to claim 2, wherein the weight percentage of the natural antioxidant moisturizing composition is 2.6% by weight. The method for preparing the skin essence for external use according to claim 1, further comprising the following steps: heating the hydrogenated lecithin and the natural anti-oxidant moisturizing composition to 65° C. to completely dissolve to obtain a first mixture, the first mixture Is the oil phase composition of the raw material liquid; glycerin, butylene glycol, diglycerin, sodium hyaluronate, allantoin, dipotassium glycyrrhizinate, disodium EDTA, deionized water is heated to 75 ℃ to completely dissolve to obtain a The second mixture, the second mixture is the water phase component of the raw material liquid; the first mixture is added to the second mixture to obtain the raw material liquid of the essence, and the raw material liquid is placed In the fixed vacuum homogenizing mixing pot, turn on the fixed vacuum homogenizing mixing blade, the mixing speed is 600rpm, the homogenizing speed is 2500rpm, and the vacuum is turned on to 0.04Mpa. After homogenizing and stirring for 10 minutes, the vacuum is continued and the temperature is reduced to 45°C and the vacuum is released. After pressure, add a colloidal silver, turn on the stirring speed of 600rpm, the homogenization speed of 2500rpm, after homogenizing and stirring for 5 minutes, continue to vacuum and stir to lower the temperature to 35°C, the semi-finished product is discharged and filtered and packed into barrels to obtain the discharge of the primary processing process for use; The pineapple protease is added to the output of the primary processing process and then put into a composite bag, the air in the bag is exhausted and then sealed, the composite bag is placed in the ultra-high pressure processing device, and the ultra-high pressure processing device is filled with water As a medium, control the temperature at 37°C, adjust the pressure to 200Mpa, start the isostatic pressure, and accelerate the enzymatic reaction of the inner material. After a maintenance time of 1 hour, adjust the pressure to 400Mpa, the isostatic pressure starts, and the inner The material accelerates the enzymatic reaction, and the pressure relief valve is opened after the holding time is 5 hours. When the pointer returns to normal 1 atm, the composite bag is sealed and opened to obtain the secondary processing process to discharge the material for use; the secondary processing process is discharged The material is fed into the inlet of the high pressure homogenizer quantitatively by a peristaltic pump at a rate of 2ml/sec. The pressure of the material is controlled at 100Mpa. The material is added to the inlet in 4 times. The temperature of the material is circulated and controlled at 3~5℃; Translucent, the viscosity is controlled at 65 cps, and then the material is filtered through the external circulation rotor pump to obtain the skin essence for external use. A biocellulose membrane sheet comprising a biocellulose membrane skin material added with the skin essence for external use according to claim 1.